Cytochrome p450
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 462 |
| AccI | GTMKAC | 1 cut(s) | 454 |
| AccII | CGCG | 2 cut(s) | 307, 339 |
| AciI | CCGC | 2 cut(s) | 29, 220 |
| AclI | AACGTT | 1 cut(s) | 215 |
| AclWI | GGATC | 1 cut(s) | 335 |
| AcsI | RAATTY | 2 cut(s) | 424, 431 |
| AcuI | CTGAAG | 1 cut(s) | 131 |
| AfaI | GTAC | 3 cut(s) | 5, 249, 304 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 25, 115, 605 |
| AflIII | ACRYGT | 1 cut(s) | 305 |
| AgsI | TTSAA | 4 cut(s) | 327, 431, 488, 530 |
| AjnI | CCWGG | 2 cut(s) | 71, 466 |
| AlwI | GGATC | 1 cut(s) | 335 |
| AoxI | GGCC | 1 cut(s) | 418 |
| ApoI | RAATTY | 2 cut(s) | 424, 431 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 384 |
| AspS9I | GGNCC | 3 cut(s) | 236, 418, 608 |
| AsuHPI | GGTGA | 1 cut(s) | 578 |
| AvaII | GGWCC | 2 cut(s) | 236, 608 |
| AxyI | CCTNAGG | 1 cut(s) | 298 |
| BanII | GRGCYC | 1 cut(s) | 79 |
| BbsI | GAAGAC | 2 cut(s) | 302, 597 |
| BccI | CCATC | 1 cut(s) | 550 |
| BciT130I | CCWGG | 2 cut(s) | 73, 468 |
| BfaI | CTAG | 4 cut(s) | 38, 185, 479, 603 |
| BisI | GCNGC | 1 cut(s) | 221 |
| BlsI | GCNGC | 1 cut(s) | 222 |
| Bme1390I | CCNGG | 2 cut(s) | 73, 468 |
| Bme18I | GGWCC | 2 cut(s) | 236, 608 |
| BmgT120I | GGNCC | 3 cut(s) | 236, 418, 608 |
| BmiI | GGNNCC | 1 cut(s) | 419 |
| BmrFI | CCNGG | 2 cut(s) | 73, 468 |
| BoxI | GACNNNNGTC | 1 cut(s) | 363 |
| BpiI | GAAGAC | 2 cut(s) | 302, 597 |
| BpuEI | CTTGAG | 1 cut(s) | 146 |
| BsaJI | CCNNGG | 1 cut(s) | 72 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 25, 115, 605 |
| Bse1I | ACTGG | 2 cut(s) | 510, 554 |
| Bse21I | CCTNAGG | 1 cut(s) | 298 |
| BseBI | CCWGG | 2 cut(s) | 73, 468 |
| BseDI | CCNNGG | 1 cut(s) | 72 |
| BseGI | GGATG | 1 cut(s) | 629 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 25, 115, 605 |
| BseMII | CTCAG | 2 cut(s) | 258, 312 |
| BseNI | ACTGG | 2 cut(s) | 510, 554 |
| BsgI | GTGCAG | 1 cut(s) | 256 |
| Bsh1236I | CGCG | 2 cut(s) | 307, 339 |
| BshFI | GGCC | 1 cut(s) | 420 |
| BslFI | GGGAC | 1 cut(s) | 58 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 25, 115, 605 |
| BsmFI | GGGAC | 1 cut(s) | 58 |
| BsnI | GGCC | 1 cut(s) | 420 |
| Bsp1286I | GDGCHC | 1 cut(s) | 79 |
| Bsp1407I | TGTACA | 1 cut(s) | 3 |
| Bsp143I | GATC | 1 cut(s) | 340 |
| Bsp68I | TCGCGA | 1 cut(s) | 339 |
| BspACI | CCGC | 2 cut(s) | 29, 220 |
| BspANI | GGCC | 1 cut(s) | 420 |
| BspCNI | CTCAG | 2 cut(s) | 257, 311 |
| BspFNI | CGCG | 2 cut(s) | 307, 339 |
| BspLI | GGNNCC | 1 cut(s) | 419 |
| BspPI | GGATC | 1 cut(s) | 335 |
| BsrGI | TGTACA | 1 cut(s) | 3 |
| BsrI | ACTGG | 2 cut(s) | 510, 554 |
| BssECI | CCNNGG | 1 cut(s) | 72 |
| BssMI | GATC | 1 cut(s) | 340 |
| Bst2UI | CCWGG | 2 cut(s) | 73, 468 |
| Bst4CI | ACNGT | 1 cut(s) | 61 |
| BstAUI | TGTACA | 1 cut(s) | 3 |
| BstC8I | GCNNGC | 1 cut(s) | 627 |
| BstDEI | CTNAG | 2 cut(s) | 244, 298 |
| BstENI | CCTNNNNNAGG | 2 cut(s) | 23, 113 |
| BstF5I | GGATG | 1 cut(s) | 629 |
| BstFNI | CGCG | 2 cut(s) | 307, 339 |
| BstKTI | GATC | 1 cut(s) | 343 |
| BstMBI | GATC | 1 cut(s) | 340 |
| BstNI | CCWGG | 2 cut(s) | 73, 468 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 363 |
| BstSCI | CCNGG | 2 cut(s) | 71, 466 |
| BstUI | CGCG | 2 cut(s) | 307, 339 |
| BstV2I | GAAGAC | 2 cut(s) | 302, 597 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 351 |
| Bsu36I | CCTNAGG | 1 cut(s) | 298 |
| BsuRI | GGCC | 1 cut(s) | 420 |
| BtgZI | GCGATG | 1 cut(s) | 320 |
| BtsCI | GGATG | 1 cut(s) | 629 |
| BtsIMutI | CAGTG | 2 cut(s) | 66, 503 |
| BtuMI | TCGCGA | 1 cut(s) | 339 |
| Cac8I | GCNNGC | 1 cut(s) | 627 |
| Cfr13I | GGNCC | 3 cut(s) | 236, 418, 608 |
| CseI | GACGC | 1 cut(s) | 296 |
| Csp6I | GTAC | 3 cut(s) | 4, 248, 303 |
| CviAII | CATG | 6 cut(s) | 140, 254, 290, 363, 521, 587 |
| CviJI | RGCY | 9 cut(s) | 77, 119, 188, 200, 223, 355, 420, 509, 539 |
| CviKI_1 | RGCY | 9 cut(s) | 77, 119, 188, 200, 223, 355, 420, 509, 539 |
| CviQI | GTAC | 3 cut(s) | 4, 248, 303 |
| DdeI | CTNAG | 2 cut(s) | 244, 298 |
| DpnI | GATC | 1 cut(s) | 342 |
| DpnII | GATC | 1 cut(s) | 340 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 462 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 462 |
| Eco24I | GRGCYC | 1 cut(s) | 79 |
| Eco47I | GGWCC | 2 cut(s) | 236, 608 |
| Eco57I | CTGAAG | 1 cut(s) | 131 |
| Eco81I | CCTNAGG | 1 cut(s) | 298 |
| EcoNI | CCTNNNNNAGG | 2 cut(s) | 23, 113 |
| EcoO109I | RGGNCCY | 1 cut(s) | 608 |
| EcoRII | CCWGG | 2 cut(s) | 71, 466 |
| EcoT38I | GRGCYC | 1 cut(s) | 79 |
| FaeI | CATG | 6 cut(s) | 143, 257, 293, 366, 524, 590 |
| FaqI | GGGAC | 1 cut(s) | 58 |
| FatI | CATG | 6 cut(s) | 139, 253, 289, 362, 520, 586 |
| FauNDI | CATATG | 1 cut(s) | 13 |
| FblI | GTMKAC | 1 cut(s) | 454 |
| Fnu4HI | GCNGC | 1 cut(s) | 221 |
| FokI | GGATG | 1 cut(s) | 616 |
| FriOI | GRGCYC | 1 cut(s) | 79 |
| Fsp4HI | GCNGC | 1 cut(s) | 221 |
| FspBI | CTAG | 4 cut(s) | 38, 185, 479, 603 |
| GluI | GCNGC | 1 cut(s) | 221 |
| HaeIII | GGCC | 1 cut(s) | 420 |
| HgaI | GACGC | 1 cut(s) | 296 |
| Hin1II | CATG | 6 cut(s) | 143, 257, 293, 366, 524, 590 |
| HinfI | GANTC | 4 cut(s) | 167, 257, 331, 471 |
| HphI | GGTGA | 1 cut(s) | 578 |
| Hpy166II | GTNNAC | 1 cut(s) | 455 |
| Hpy188I | TCNGA | 2 cut(s) | 172, 547 |
| Hpy188III | TCNNGA | 4 cut(s) | 109, 261, 338, 574 |
| Hpy8I | GTNNAC | 1 cut(s) | 455 |
| HpyAV | CCTTC | 2 cut(s) | 109, 621 |
| HpyCH4III | ACNGT | 1 cut(s) | 61 |
| HpyCH4IV | ACGT | 1 cut(s) | 215 |
| HpyCH4V | TGCA | 2 cut(s) | 273, 629 |
| HpyF3I | CTNAG | 2 cut(s) | 244, 298 |
| HpySE526I | ACGT | 1 cut(s) | 215 |
| Hsp92II | CATG | 6 cut(s) | 143, 257, 293, 366, 524, 590 |
| Kzo9I | GATC | 1 cut(s) | 340 |
| MaeI | CTAG | 4 cut(s) | 38, 185, 479, 603 |
| MaeII | ACGT | 1 cut(s) | 215 |
| MaeIII | GTNAC | 5 cut(s) | 61, 87, 281, 292, 490 |
| MalI | GATC | 1 cut(s) | 342 |
| MboI | GATC | 1 cut(s) | 340 |
| MboII | GAAGA | 5 cut(s) | 26, 124, 190, 302, 602 |
| MhlI | GDGCHC | 1 cut(s) | 79 |
| MluCI | AATT | 3 cut(s) | 424, 431, 531 |
| MluI | ACGCGT | 1 cut(s) | 305 |
| MmeI | TCCRAC | 2 cut(s) | 111, 397 |
| MnlI | CCTC | 6 cut(s) | 19, 35, 64, 249, 307, 599 |
| MroXI | GAANNNNTTC | 1 cut(s) | 384 |
| MseI | TTAA | 1 cut(s) | 68 |
| MslI | CAYNNNNRTG | 1 cut(s) | 402 |
| MspR9I | CCNGG | 2 cut(s) | 73, 468 |
| MvaI | CCWGG | 2 cut(s) | 73, 468 |
| MvnI | CGCG | 2 cut(s) | 307, 339 |
| NdeI | CATATG | 1 cut(s) | 13 |
| NdeII | GATC | 1 cut(s) | 340 |
| NlaIII | CATG | 6 cut(s) | 143, 257, 293, 366, 524, 590 |
| NlaIV | GGNNCC | 1 cut(s) | 419 |
| NmuCI | GTSAC | 1 cut(s) | 61 |
| NruI | TCGCGA | 1 cut(s) | 339 |
| PdmI | GAANNNNTTC | 1 cut(s) | 384 |
| PfeI | GAWTC | 4 cut(s) | 167, 257, 331, 471 |
| PfoI | TCCNGGA | 1 cut(s) | 466 |
| PkrI | GCNGC | 1 cut(s) | 222 |
| PpuMI | RGGWCCY | 1 cut(s) | 608 |
| PshAI | GACNNNNGTC | 1 cut(s) | 363 |
| Psp1406I | AACGTT | 1 cut(s) | 215 |
| Psp5II | RGGWCCY | 1 cut(s) | 608 |
| Psp6I | CCWGG | 2 cut(s) | 71, 466 |
| PspGI | CCWGG | 2 cut(s) | 71, 466 |
| PspN4I | GGNNCC | 1 cut(s) | 419 |
| PspPI | GGNCC | 3 cut(s) | 236, 418, 608 |
| PspPPI | RGGWCCY | 1 cut(s) | 608 |
| RruI | TCGCGA | 1 cut(s) | 339 |
| RsaI | GTAC | 3 cut(s) | 5, 249, 304 |
| RsaNI | GTAC | 3 cut(s) | 4, 248, 303 |
| RseI | CAYNNNNRTG | 1 cut(s) | 402 |
| SaqAI | TTAA | 1 cut(s) | 68 |
| SatI | GCNGC | 1 cut(s) | 221 |
| Sau3AI | GATC | 1 cut(s) | 340 |
| Sau96I | GGNCC | 3 cut(s) | 236, 418, 608 |
| ScrFI | CCNGG | 2 cut(s) | 73, 468 |
| SduI | GDGCHC | 1 cut(s) | 79 |
| SinI | GGWCC | 2 cut(s) | 236, 608 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 402 |
| SmlI | CTYRAG | 1 cut(s) | 125 |
| SmoI | CTYRAG | 1 cut(s) | 125 |
| Sse9I | AATT | 3 cut(s) | 424, 431, 531 |
| SsiI | CCGC | 2 cut(s) | 29, 220 |
| SspMI | CTAG | 4 cut(s) | 38, 185, 479, 603 |
| StyD4I | CCNGG | 2 cut(s) | 71, 466 |
| TaaI | ACNGT | 1 cut(s) | 61 |
| TaiI | ACGT | 1 cut(s) | 218 |
| TaqI | TCGA | 1 cut(s) | 357 |
| TasI | AATT | 3 cut(s) | 424, 431, 531 |
| TatI | WGTACW | 1 cut(s) | 3 |
| TauI | GCSGC | 1 cut(s) | 223 |
| TfiI | GAWTC | 4 cut(s) | 167, 257, 331, 471 |
| Tru1I | TTAA | 1 cut(s) | 68 |
| Tru9I | TTAA | 1 cut(s) | 68 |
| TscAI | CASTG | 2 cut(s) | 66, 510 |
| TseFI | GTSAC | 1 cut(s) | 61 |
| Tsp45I | GTSAC | 1 cut(s) | 61 |
| TspDTI | ATGAA | 6 cut(s) | 180, 270, 323, 509, 564, 603 |
| TspRI | CASTG | 2 cut(s) | 66, 510 |
| VpaK11BI | GGWCC | 2 cut(s) | 236, 608 |
| XagI | CCTNNNNNAGG | 2 cut(s) | 23, 113 |
| XapI | RAATTY | 2 cut(s) | 424, 431 |
| XmiI | GTMKAC | 1 cut(s) | 454 |
| XmnI | GAANNNNTTC | 1 cut(s) | 384 |
| XspI | CTAG | 4 cut(s) | 38, 185, 479, 603 |