Rw6G035520

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0000137
Physical Location & Seq
Forward (+)
60129988 .. 60132636
2649 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G035520.1

Sequence Viewer

Length: 1617 bp
ATGGAGTTCCCTCTTCAATATCTCAGTTCTTCTCCATCCACCATACTTGGCTTTTTATTGTTTATTCTCTCTTTATTGCTATGGATAACAAAAATTTCCAACAATAACGCAGCCAGAAAGAGGGGAGCACCGGAAGCAGCCGGTGCATGGCCATTAGTTGGTCACCTTCCCCTCTTAGGCGGCTCACTTCCGCCGCATATAACCTTGGGTAACATGGCTGACAAGTATGGACCGCTCTTCACAATCAAACTCGGCGTGCACCGATCTCTTATTGTGAGCAGTTGGGACATGGCCAAAGGGTGTCTAACCACCAACGACAGAGTCTTTGCTAATCGCCCGAAGCTATTAGTCTCAGAGGTTATGGGATACAACTACGCCATGATTGGGTTCAGTCCCTATGGTCCTTACTGGCGCCAGATGCGCAAGGTAGCCACCCTGGAGCTCCTCTCGAACTACCGCTTGGAGAACCTCAGGCACGTTCGTGAATCCGAGGTGAAGGCTTGTACCAAGAACATTTACGATTTGTGGAGGCAAAACAAACGCTCAGATCATGTTTTGGTGGACATGAAGAGGTGGTTCGGAGACGTAACGGTGAACACCATGTTTAGGATGGTTATAGGGAAGCGGTTCCTACTTGAGGCTAGCTCGGAGCGTGAGAAGGAAGAGAACGAAAGGTTTCGAAAGGCGTTGAGGGAGTTTTTCAGATTGGCCGGTGAGTTTGTGATGGCGGATGCAGTGCCTTTCCTTAGGTGGTTAGATTTGGGCGGACATGAGAAGGCAATGAAGAAGACGGCAAAGGAACTAGATCATGTTCTGGAACGATGGTTGGAAGAGCATAAGCTAAAACGTAGTACTAATTCTACTTCTACTTCGCTTGAGCCTGACGATTTCATGGATATGATGCTTTCTGTTCTTGATGATAGTATACTGGAGTTTCGTGGGTACACTACTGCTGACACCATCAACAAAGCTACTTGTCTGGCTCTTATATTAGGGGGAACAGACACCACAACAGTAACATTAACATGGGCTCTTTCATTACTAGTGAACAACCCTTCAATCTTAAAGAAAGCTCAAAATGAATTAGACACAAAAATTGGCCGGAATAGACAAGTGAATGAATCAGACGTGAAGAACCTCGTCTATCTCCAAGCCATTATCAAAGAAACCATGCGCTTATACCCTGCTGTACCACTCCTCGCACCCCATGAATCCACAGAAGACTGCACCGTAGGAAACTACCATGTACCCGCCAAAACACGTCTAATCGTCAATATTTGGAAGCTTCACCGGGATCCGAATGTCTGGCTAGATCCCTGCATTTTCCAGCCTGAAAGGTTCCTTACAAGTCATGAGGATTTTGATGTTAGGGGCCAAAATTTTGAGCTCATACCGTTTGGAAGTGGTAGAAGAATATGTCCAGGAATTTCTTTGGCACTTCAAGTTATAGAACTCACTCTAGCTCATCTGTTGCATGGGTTTGAAATTACAGCCCCATCAGATGAACCGGTCGATATGAGTGAGACTGTTGGGTTAACAAACATGAAGGCCACACCATTAGAGGTCCTTCTCAGACCACGCCTTCCTGCTGACATTTATGAATACGTCTCGTTATAA

Protein Analysis

538

Amino Acids

61.23

Weight (kDa)

7.68

Isoelectric Point (pI)

45.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 48 - 513 4e-94 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000137)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G31940 AT4G31950 AT4G31970
fragaria_vesca FvH4_1g17460 FvH4_2g40550 FvH4_2g40551 FvH4_2g40560 FvH4_2g40570 FvH4_2g40580 FvH4_2g40590 FvH4_7g16520 FvH4_7g16601 FvH4_7g16602 FvH4_7g22620 FvH4_7g22810
malus_domestica MD00G1037400.v1.1 MD00G1037500.v1.1 MD00G1175000.v1.1 MD00G1175100.v1.1 MD00G1175200.v1.1 MD03G1091600.v1.1 MD04G1043800.v1.1 MD04G1043900.v1.1 MD04G1044100.v1.1 MD04G1044200.v1.1 MD04G1044300.v1.1 MD04G1044400.v1.1 MD08G1234700.v1.1 MD14G1047100.v1.1 MD14G1163500.v1.1 MD15G1028200.v1.1 MD15G1028300.v1.1 MD15G1028400.v1.1 MD15G1028500.v1.1 MD15G1028700.v1.1 MD15G1032900.v1.1 MD15G1033100.v1.1 MD15G1033400.v1.1 MD15G1033800.v1.1 MD15G1033900.v1.1 MD15G1378500.v1.1 MD15G1378800.v1.1
prunus_persica Prupe.1G386800_v2.0.a1 Prupe.1G386800_v2.0.a1 Prupe.1G386900_v2.0.a1 Prupe.1G387000_v2.0.a1 Prupe.1G387100_v2.0.a1 Prupe.1G387200_v2.0.a1 Prupe.1G387300_v2.0.a1 Prupe.1G387400_v2.0.a1 Prupe.1G387500_v2.0.a1 Prupe.1G387700_v2.0.a1 Prupe.1G537400_v2.0.a1 Prupe.1G537600_v2.0.a1 Prupe.1G537700_v2.0.a1 Prupe.1G538000_v2.0.a1 Prupe.1G538200_v2.0.a1 Prupe.3G064700_v2.0.a1 Prupe.3G066200_v2.0.a1 Prupe.6G212300_v2.0.a1
pyrus_communis pycom04g03800 pycom04g03820 pycom09g19430 pycom11g16760 pycom14g13610 pycom15g02430 pycom15g02460 pycom15g02470 pycom15g02480 pycom15g02520 pycom15g03050 pycom15g03060 pycom15g03070 pycom15g03090 pycom15g03100 pycom15g03110 pycom15g03120 pycom15g33920
rosa_chinensis RchiOBHm_Chr1g0359661 RchiOBHm_Chr1g0359671 RchiOBHm_Chr1g0359711 RchiOBHm_Chr1g0359751 RchiOBHm_Chr1g0359791 RchiOBHm_Chr2g0107671 RchiOBHm_Chr2g0107681 RchiOBHm_Chr2g0107691 RchiOBHm_Chr5g0058861 RchiOBHm_Chr5g0058871 RchiOBHm_Chr5g0058891 RchiOBHm_Chr5g0058901 RchiOBHm_Chr5g0058921 RchiOBHm_Chr5g0058931 RchiOBHm_Chr5g0058941 RchiOBHm_Chr5g0058971 RchiOBHm_Chr5g0058991 RchiOBHm_Chr5g0059001 RchiOBHm_Chr5g0060081 RchiOBHm_Chr6g0300111 RchiOBHm_Chr6g0300121 RchiOBHm_Chr6g0300131 RchiOBHm_Chr6g0300141 RchiOBHm_Chr6g0300151 RchiOBHm_Chr6g0300161 RchiOBHm_Chr7g0223401 RchiOBHm_Chr7g0223411 RchiOBHm_Chr7g0233631
rosa_laevigata RLG00000001247 RLG00000011362 RLG00000011363 RLG00000011364 RLG00000011365 RLG00000011367 RLG00000017599 RLG00000017600 RLG00000027854 RLG00000027856 RLG00000027857 RLG00000035257 RLG00000035258 RLG00000035263 RLG00000035267 RLG00000035268 RLG00000035270 RLG00000035272 RLG00000035273 RLG00000035275 RLG00000035276 RLG00000035343
rosa_multiflora Rmu_co8382255.1_g000001 Rmu_sc0000441.1_g000120 Rmu_sc0000441.1_g000122 Rmu_sc0000493.1_g000037 Rmu_sc0000493.1_g000038 Rmu_sc0001670.1_g000042 Rmu_sc0001670.1_g000043 Rmu_sc0001670.1_g000044 Rmu_sc0002983.1_g000067 Rmu_sc0002983.1_g000074 Rmu_sc0003366.1_g000019 Rmu_sc0003494.1_g000002 Rmu_sc0003712.1_g000014 Rmu_sc0005703.1_g000003 Rmu_sc0005703.1_g000004 Rmu_sc0006429.1_g000014 Rmu_sc0006429.1_g000018 Rmu_sc0006429.1_g000019 Rmu_sc0008701.1_g000007 Rmu_sc0009050.1_g000002 Rmu_sc0009050.1_g000008 Rmu_sc0014587.1_g000001 Rmu_sc0034954.1_g000001 Rmu_ssc0000371.1_g000008 Rmu_ssc0000371.1_g000009 Rmu_ssc0000371.1_g000012 Rmu_ssc0000371.1_g000025 Rmu_ssc0000371.1_g000028
rosa_roxburghii Rroxscaffold_1G00021390 Rroxscaffold_1G00021450 Rroxscaffold_1G00021460 Rroxscaffold_1G00021470 Rroxscaffold_1G00021480 Rroxscaffold_1G00021500 Rroxscaffold_2G00136030 Rroxscaffold_2G00136040 Rroxscaffold_2G00136070 Rroxscaffold_3G00227100 Rroxscaffold_4G00296830 Rroxscaffold_4G00296860 Rroxscaffold_4G00296870 Rroxscaffold_7G00168050 Rroxscaffold_7G00168060 Rroxscaffold_7G00168080 Rroxscaffold_7G00168090
rosa_rugosa Rorug01G0272400 Rorug01G0272500 Rorug01G0272600 Rorug01G0272700 Rorug02G0146800.1 Rorug03G0204200 Rorug03G0204300 Rorug05G0319800 Rorug05G0319900 Rorug06G0293800 Rorug06G0293800 Rorug06G0293800 Rorug06G0293800 Rorug07G0281800
rosa_samantha Rh5DG412300 Rh5DG412400 Rh6CG421000 Rh7DG427300
rosa_wichuraiana Rw1G025420 Rw1G025430 Rw1G025440 Rw2G015520 Rw5G036240 Rw5G036250 Rw5G036260 Rw5G036270 Rw5G036950 Rw6G035500 Rw6G035510 Rw6G035520 Rw7G036140

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1615
Acc16I TGCGCA 1 cut(s) 422
AccB1I GGYRCC 1 cut(s) 411
AccB7I CCANNNNNTGG 1 cut(s) 158
AccBSI CCGCTC 1 cut(s) 235
AccI GTMKAC 1 cut(s) 925
AciI CCGC 9 cut(s) 180, 191, 194, 233, 457, 625, 728, 765, 1251
AclWI GGATC 3 cut(s) 1289, 1302, 1307
AcoI YGGCCR 4 cut(s) 149, 291, 708, 1099
AcsI RAATTY 3 cut(s) 93, 1378, 1425
AcyI GRCGYC 1 cut(s) 412
AfaI GTAC 5 cut(s) 505, 853, 944, 1191, 1248
AfiI CCNNNNNNNGG 7 cut(s) 120, 147, 158, 176, 384, 606, 637
AflIII ACRYGT 1 cut(s) 1259
AgeI ACCGGT 1 cut(s) 1507
AgsI TTSAA 4 cut(s) 17, 1059, 1442, 1484
AhlI ACTAGT 1 cut(s) 1042
AjiI CACGTC 2 cut(s) 1129, 1262
AjnI CCWGG 2 cut(s) 435, 1420
AjuI GAANNNNNNNTTGG 2 cut(s) 443, 475
AleI CACNNNNGTG 1 cut(s) 480
AluBI AGCT 9 cut(s) 343, 442, 645, 841, 971, 1073, 1285, 1387, 1463
AluI AGCT 9 cut(s) 343, 442, 645, 841, 971, 1073, 1285, 1387, 1463
Alw21I GWGCWC 4 cut(s) 130, 261, 444, 1389
Alw26I GTCTC 4 cut(s) 355, 576, 1517, 1612
Alw44I GTGCAC 1 cut(s) 257
AlwI GGATC 3 cut(s) 1289, 1302, 1307
AoxI GGCC 6 cut(s) 149, 291, 708, 1099, 1372, 1548
ApaLI GTGCAC 1 cut(s) 257
ApeKI GCWGC 2 cut(s) 110, 137
ApoI RAATTY 3 cut(s) 93, 1378, 1425
ArsI GACNNNNNNTTYG 2 cut(s) 308, 340
AsiGI ACCGGT 1 cut(s) 1507
Asp700I GAANNNNTTC 2 cut(s) 626, 675
AspLEI GCGC 3 cut(s) 414, 423, 1176
AspS9I GGNCC 4 cut(s) 230, 401, 1372, 1564
AsuC2I CCSGG 1 cut(s) 1292
AsuHPI GGTGA 5 cut(s) 155, 505, 604, 725, 1280
AsuII TTCGAA 1 cut(s) 679
AsuNHI GCTAGC 1 cut(s) 641
AvaII GGWCC 3 cut(s) 230, 401, 1564
AxyI CCTNAGG 2 cut(s) 470, 746
BaeGI GKGCMC 1 cut(s) 261
BalI TGGCCA 2 cut(s) 151, 293
BamHI GGATCC 1 cut(s) 1294
BanI GGYRCC 1 cut(s) 411
BanII GRGCYC 3 cut(s) 444, 1033, 1389
BbsI GAAGAC 2 cut(s) 794, 1227
Bbv12I GWGCWC 4 cut(s) 130, 261, 444, 1389
BbvI GCAGC 2 cut(s) 122, 149
BccI CCATC 6 cut(s) 43, 604, 718, 816, 968, 1504
BceAI ACGGC 1 cut(s) 807
BciT130I CCWGG 2 cut(s) 437, 1422
BciVI GTATCC 1 cut(s) 359
BcnI CCSGG 1 cut(s) 1292
BcoDI GTCTC 4 cut(s) 355, 576, 1517, 1612
BcuI ACTAGT 1 cut(s) 1042
BfaI CTAG 5 cut(s) 642, 803, 1043, 1310, 1460
BfoI RGCGCY 1 cut(s) 415
BfuI GTATCC 1 cut(s) 359
BisI GCNGC 4 cut(s) 111, 138, 181, 194
BlsI GCNGC 4 cut(s) 112, 139, 182, 195
BmcAI AGTACT 1 cut(s) 853
Bme1390I CCNGG 3 cut(s) 437, 1292, 1422
Bme18I GGWCC 3 cut(s) 230, 401, 1564
BmgBI CACGTC 2 cut(s) 1129, 1262
BmgT120I GGNCC 4 cut(s) 230, 401, 1372, 1564
BmiI GGNNCC 5 cut(s) 413, 629, 1296, 1340, 1373
BmrFI CCNGG 3 cut(s) 437, 1292, 1422
BmsI GCATC 3 cut(s) 408, 721, 891
BmtI GCTAGC 1 cut(s) 645
BpiI GAAGAC 2 cut(s) 794, 1227
BplI GAGNNNNNCTC 4 cut(s) 431, 463, 629, 661
BpmI CTGGAG 2 cut(s) 458, 950
Bpu14I TTCGAA 1 cut(s) 679
BpuEI CTTGAG 2 cut(s) 656, 896
BpuMI CCSGG 1 cut(s) 1292
BsaHI GRCGYC 1 cut(s) 412
BsaJI CCNNGG 3 cut(s) 204, 435, 489
BsaWI WCCGGW 2 cut(s) 130, 1507
Bsc4I CCNNNNNNNGG 7 cut(s) 120, 147, 158, 176, 384, 606, 637
Bse118I RCCGGY 3 cut(s) 140, 710, 1507
Bse1I ACTGG 2 cut(s) 413, 933
Bse21I CCTNAGG 2 cut(s) 470, 746
Bse3DI GCAATG 1 cut(s) 786
BseBI CCWGG 2 cut(s) 437, 1422
BseDI CCNNGG 3 cut(s) 204, 435, 489
BseGI GGATG 3 cut(s) 35, 615, 736
BseLI CCNNNNNNNGG 7 cut(s) 120, 147, 158, 176, 384, 606, 637
BseMI GCAATG 1 cut(s) 786
BseMII CTCAG 5 cut(s) 37, 366, 484, 558, 1585
BseNI ACTGG 2 cut(s) 413, 933
BseRI GAGGAG 2 cut(s) 434, 1187
BseSI GKGCMC 1 cut(s) 261
BseXI GCAGC 2 cut(s) 122, 149
BsgI GTGCAG 1 cut(s) 1210
Bsh1285I CGRYCG 1 cut(s) 1512
BshFI GGCC 6 cut(s) 151, 293, 710, 1101, 1374, 1550
BshNI GGYRCC 1 cut(s) 411
BshTI ACCGGT 1 cut(s) 1507
BsiEI CGRYCG 1 cut(s) 1512
BsiHKAI GWGCWC 4 cut(s) 130, 261, 444, 1389
BsiSI CCGG 6 cut(s) 131, 141, 711, 1102, 1291, 1508
BslFI GGGAC 2 cut(s) 299, 378
BslI CCNNNNNNNGG 7 cut(s) 120, 147, 158, 176, 384, 606, 637
BsmAI GTCTC 4 cut(s) 355, 576, 1517, 1612
BsmBI CGTCTC 2 cut(s) 576, 1612
BsmFI GGGAC 2 cut(s) 299, 378
BsnI GGCC 6 cut(s) 151, 293, 710, 1101, 1374, 1550
Bsp119I TTCGAA 1 cut(s) 679
Bsp1286I GDGCHC 5 cut(s) 130, 261, 444, 1033, 1389
Bsp143I GATC 5 cut(s) 263, 547, 805, 1294, 1312
BspACI CCGC 9 cut(s) 180, 191, 194, 233, 457, 625, 728, 765, 1251
BspANI GGCC 6 cut(s) 151, 293, 710, 1101, 1374, 1550
BspCNI CTCAG 5 cut(s) 36, 365, 483, 557, 1584
BspHI TCATGA 1 cut(s) 1351
BspLI GGNNCC 5 cut(s) 413, 629, 1296, 1340, 1373
BspOI GCTAGC 1 cut(s) 645
BspPI GGATC 3 cut(s) 1289, 1302, 1307
BspQI GCTCTTC 2 cut(s) 242, 825
BspT104I TTCGAA 1 cut(s) 679
BspT107I GGYRCC 1 cut(s) 411
BsrBI CCGCTC 1 cut(s) 235
BsrDI GCAATG 1 cut(s) 786
BsrFI RCCGGY 3 cut(s) 140, 710, 1507
BsrI ACTGG 2 cut(s) 413, 933
BssAI RCCGGY 3 cut(s) 140, 710, 1507
BssECI CCNNGG 3 cut(s) 204, 435, 489
BssMI GATC 5 cut(s) 263, 547, 805, 1294, 1312
BssNAI GTATAC 1 cut(s) 926
BssNI GRCGYC 1 cut(s) 412
BssT1I CCWWGG 1 cut(s) 204
Bst1107I GTATAC 1 cut(s) 926
Bst2UI CCWGG 2 cut(s) 437, 1422
Bst4CI ACNGT 5 cut(s) 592, 1015, 1231, 1395, 1528
Bst6I CTCTTC 5 cut(s) 18, 242, 563, 657, 825
BstACI GRCGYC 1 cut(s) 412
BstAPI GCANNNNNTGC 1 cut(s) 143
BstBI TTCGAA 1 cut(s) 679
BstC8I GCNNGC 2 cut(s) 257, 643
BstDEI CTNAG 7 cut(s) 23, 175, 352, 470, 544, 746, 1571
BstEII GGTNACC 1 cut(s) 161
BstENI CCTNNNNNAGG 1 cut(s) 635
BstF5I GGATG 3 cut(s) 35, 615, 736
BstH2I RGCGCY 1 cut(s) 415
BstHHI GCGC 3 cut(s) 414, 423, 1176
BstKTI GATC 5 cut(s) 266, 550, 808, 1297, 1315
BstMAI GTCTC 4 cut(s) 355, 576, 1517, 1612
BstMBI GATC 5 cut(s) 263, 547, 805, 1294, 1312
BstMCI CGRYCG 1 cut(s) 1512
BstMWI GCNNNNNNNGC 4 cut(s) 134, 143, 418, 420
BstNI CCWGG 2 cut(s) 437, 1422
BstPI GGTNACC 1 cut(s) 161
BstSCI CCNGG 3 cut(s) 435, 1290, 1420
BstSLI GKGCMC 1 cut(s) 261
BstV1I GCAGC 2 cut(s) 122, 149
BstV2I GAAGAC 2 cut(s) 794, 1227
BstX2I RGATCY 2 cut(s) 1294, 1312
BstYI RGATCY 2 cut(s) 1294, 1312
BstZ17I GTATAC 1 cut(s) 926
Bsu36I CCTNAGG 2 cut(s) 470, 746
BsuI GTATCC 1 cut(s) 359
BsuRI GGCC 6 cut(s) 151, 293, 710, 1101, 1374, 1550
BtrI CACGTC 2 cut(s) 1129, 1262
BtsCI GGATG 3 cut(s) 35, 615, 736
BtsI GCAGTG 1 cut(s) 741
BtsIMutI CAGTG 1 cut(s) 741
Cac8I GCNNGC 2 cut(s) 257, 643
CciI TCATGA 1 cut(s) 1351
CfoI GCGC 3 cut(s) 414, 423, 1176
Cfr10I RCCGGY 3 cut(s) 140, 710, 1507
Cfr13I GGNCC 4 cut(s) 230, 401, 1372, 1564
Csp6I GTAC 5 cut(s) 504, 852, 943, 1190, 1247
CspAI ACCGGT 1 cut(s) 1507
CspCI CAANNNNNGTGG 2 cut(s) 28, 63
CviQI GTAC 5 cut(s) 504, 852, 943, 1190, 1247
DdeI CTNAG 7 cut(s) 23, 175, 352, 470, 544, 746, 1571
DinI GGCGCC 1 cut(s) 413
DpnI GATC 5 cut(s) 265, 549, 807, 1296, 1314
DpnII GATC 5 cut(s) 263, 547, 805, 1294, 1312
EaeI YGGCCR 4 cut(s) 149, 291, 708, 1099
Eam1104I CTCTTC 5 cut(s) 18, 242, 563, 657, 825
EarI CTCTTC 5 cut(s) 18, 242, 563, 657, 825
EciI GGCGGA 3 cut(s) 180, 743, 780
Ecl136II GAGCTC 2 cut(s) 442, 1387
Eco130I CCWWGG 1 cut(s) 204
Eco24I GRGCYC 3 cut(s) 444, 1033, 1389
Eco47I GGWCC 3 cut(s) 230, 401, 1564
Eco53kI GAGCTC 2 cut(s) 442, 1387
Eco81I CCTNAGG 2 cut(s) 470, 746
Eco91I GGTNACC 1 cut(s) 161
EcoICRI GAGCTC 2 cut(s) 442, 1387
EcoNI CCTNNNNNAGG 1 cut(s) 635
EcoO109I RGGNCCY 1 cut(s) 1564
EcoO65I GGTNACC 1 cut(s) 161
EcoRII CCWGG 2 cut(s) 435, 1420
EcoT14I CCWWGG 1 cut(s) 204
EcoT38I GRGCYC 3 cut(s) 444, 1033, 1389
EgeI GGCGCC 1 cut(s) 413
EheI GGCGCC 1 cut(s) 413
ErhI CCWWGG 1 cut(s) 204
Esp3I CGTCTC 2 cut(s) 576, 1612
FaqI GGGAC 2 cut(s) 299, 378
FauI CCCGC 1 cut(s) 1258
FblI GTMKAC 1 cut(s) 925
Fnu4HI GCNGC 4 cut(s) 111, 138, 181, 194
FokI GGATG 3 cut(s) 22, 622, 743
FriOI GRGCYC 3 cut(s) 444, 1033, 1389
Fsp4HI GCNGC 4 cut(s) 111, 138, 181, 194
FspBI CTAG 5 cut(s) 642, 803, 1043, 1310, 1460
FspI TGCGCA 1 cut(s) 422
GlaI GCGC 3 cut(s) 413, 422, 1175
GluI GCNGC 4 cut(s) 111, 138, 181, 194
GsuI CTGGAG 2 cut(s) 458, 950
HaeII RGCGCY 1 cut(s) 415
HaeIII GGCC 6 cut(s) 151, 293, 710, 1101, 1374, 1550
HapII CCGG 6 cut(s) 131, 141, 711, 1102, 1291, 1508
HhaI GCGC 3 cut(s) 414, 423, 1176
Hin1I GRCGYC 1 cut(s) 412
Hin6I GCGC 3 cut(s) 412, 421, 1174
HinP1I GCGC 3 cut(s) 412, 421, 1174
HincII GTYRAC 1 cut(s) 1536
HindII GTYRAC 1 cut(s) 1536
HindIII AAGCTT 1 cut(s) 1283
HinfI GANTC 4 cut(s) 321, 485, 1121, 1211
HpaI GTTAAC 1 cut(s) 1536
HpaII CCGG 6 cut(s) 131, 141, 711, 1102, 1291, 1508
HphI GGTGA 5 cut(s) 155, 505, 604, 725, 1280
Hpy166II GTNNAC 7 cut(s) 259, 562, 595, 926, 945, 1048, 1536
Hpy188III TCNNGA 5 cut(s) 448, 482, 815, 914, 1352
Hpy8I GTNNAC 7 cut(s) 259, 562, 595, 926, 945, 1048, 1536
HpyAV CCTTC 8 cut(s) 176, 490, 652, 769, 1065, 1540, 1577, 1592
HpyCH4III ACNGT 5 cut(s) 592, 1015, 1231, 1395, 1528
HpyCH4IV ACGT 6 cut(s) 477, 585, 847, 1128, 1261, 1605
HpyCH4V TGCA 6 cut(s) 146, 259, 734, 1227, 1320, 1474
HpyF10VI GCNNNNNNNGC 4 cut(s) 134, 143, 418, 420
HpyF3I CTNAG 7 cut(s) 23, 175, 352, 470, 544, 746, 1571
HpySE526I ACGT 6 cut(s) 477, 585, 847, 1128, 1261, 1605
Hsp92I GRCGYC 1 cut(s) 412
HspAI GCGC 3 cut(s) 412, 421, 1174
KasI GGCGCC 1 cut(s) 411
KspAI GTTAAC 1 cut(s) 1536
Kzo9I GATC 5 cut(s) 263, 547, 805, 1294, 1312
LguI GCTCTTC 2 cut(s) 242, 825
LmnI GCTCC 4 cut(s) 125, 439, 447, 649
Lsp1109I GCAGC 2 cut(s) 122, 149
LweI GCATC 3 cut(s) 408, 721, 891
MaeI CTAG 5 cut(s) 642, 803, 1043, 1310, 1460
MaeII ACGT 6 cut(s) 477, 585, 847, 1128, 1261, 1605
MaeIII GTNAC 4 cut(s) 161, 209, 586, 1015
MalI GATC 5 cut(s) 265, 549, 807, 1296, 1314
MbiI CCGCTC 1 cut(s) 235
MboI GATC 5 cut(s) 263, 547, 805, 1294, 1312
MflI RGATCY 2 cut(s) 1294, 1312
MhlI GDGCHC 5 cut(s) 130, 261, 444, 1033, 1389
MlsI TGGCCA 2 cut(s) 151, 293
MluCI AATT 7 cut(s) 93, 856, 1082, 1095, 1378, 1425, 1485
MluNI TGGCCA 2 cut(s) 151, 293
Mly113I GGCGCC 1 cut(s) 412
MlyI GAGTC 1 cut(s) 330
MmeI TCCRAC 2 cut(s) 123, 807
Mox20I TGGCCA 2 cut(s) 151, 293
MroXI GAANNNNTTC 2 cut(s) 626, 675
MscI TGGCCA 2 cut(s) 151, 293
MseI TTAA 3 cut(s) 1022, 1064, 1535
MslI CAYNNNNRTG 3 cut(s) 480, 896, 1024
Msp20I TGGCCA 2 cut(s) 151, 293
MspI CCGG 6 cut(s) 131, 141, 711, 1102, 1291, 1508
MspR9I CCNGG 3 cut(s) 437, 1292, 1422
MvaI CCWGG 2 cut(s) 437, 1422
MwoI GCNNNNNNNGC 4 cut(s) 134, 143, 418, 420
NarI GGCGCC 1 cut(s) 412
NciI CCSGG 1 cut(s) 1292
NdeII GATC 5 cut(s) 263, 547, 805, 1294, 1312
NheI GCTAGC 1 cut(s) 641
NlaIV GGNNCC 5 cut(s) 413, 629, 1296, 1340, 1373
NmeAIII GCCGAG 1 cut(s) 231
NmuCI GTSAC 1 cut(s) 161
NsbI TGCGCA 1 cut(s) 422
NspV TTCGAA 1 cut(s) 679
OliI CACNNNNGTG 1 cut(s) 480
PagI TCATGA 1 cut(s) 1351
PciSI GCTCTTC 2 cut(s) 242, 825
PdmI GAANNNNTTC 2 cut(s) 626, 675
PfeI GAWTC 3 cut(s) 485, 1121, 1211
PflFI GACNNNGTC 1 cut(s) 320
PflMI CCANNNNNTGG 1 cut(s) 158
PfoI TCCNGGA 1 cut(s) 1420
PinAI ACCGGT 1 cut(s) 1507
PkrI GCNGC 4 cut(s) 112, 139, 182, 195
PleI GAGTC 1 cut(s) 329
PluTI GGCGCC 1 cut(s) 415
PpsI GAGTC 1 cut(s) 329
PpuMI RGGWCCY 1 cut(s) 1564
PsiI TTATAA 1 cut(s) 1615
Psp124BI GAGCTC 2 cut(s) 444, 1389
Psp5II RGGWCCY 1 cut(s) 1564
Psp6I CCWGG 2 cut(s) 435, 1420
PspEI GGTNACC 1 cut(s) 161
PspGI CCWGG 2 cut(s) 435, 1420
PspN4I GGNNCC 5 cut(s) 413, 629, 1296, 1340, 1373
PspPI GGNCC 4 cut(s) 230, 401, 1372, 1564
PspPPI RGGWCCY 1 cut(s) 1564
PsuI RGATCY 2 cut(s) 1294, 1312
PsyI GACNNNGTC 1 cut(s) 320
RsaI GTAC 5 cut(s) 505, 853, 944, 1191, 1248
RsaNI GTAC 5 cut(s) 504, 852, 943, 1190, 1247
RseI CAYNNNNRTG 3 cut(s) 480, 896, 1024
SacI GAGCTC 2 cut(s) 444, 1389
SapI GCTCTTC 2 cut(s) 242, 825
SaqAI TTAA 3 cut(s) 1022, 1064, 1535
SatI GCNGC 4 cut(s) 111, 138, 181, 194
Sau3AI GATC 5 cut(s) 263, 547, 805, 1294, 1312
Sau96I GGNCC 4 cut(s) 230, 401, 1372, 1564
ScaI AGTACT 1 cut(s) 853
SchI GAGTC 1 cut(s) 330
ScrFI CCNGG 3 cut(s) 437, 1292, 1422
SduI GDGCHC 5 cut(s) 130, 261, 444, 1033, 1389
SfaNI GCATC 3 cut(s) 408, 721, 891
SfoI GGCGCC 1 cut(s) 413
SfuI TTCGAA 1 cut(s) 679
SinI GGWCC 3 cut(s) 230, 401, 1564
SmiMI CAYNNNNRTG 3 cut(s) 480, 896, 1024
SmlI CTYRAG 2 cut(s) 635, 875
SmoI CTYRAG 2 cut(s) 635, 875
SpeI ACTAGT 1 cut(s) 1042
Sse9I AATT 7 cut(s) 93, 856, 1082, 1095, 1378, 1425, 1485
SsiI CCGC 9 cut(s) 180, 191, 194, 233, 457, 625, 728, 765, 1251
SspDI GGCGCC 1 cut(s) 411
SspI AATATT 1 cut(s) 1276
SspMI CTAG 5 cut(s) 642, 803, 1043, 1310, 1460
SstI GAGCTC 2 cut(s) 444, 1389
StyD4I CCNGG 3 cut(s) 435, 1290, 1420
StyI CCWWGG 1 cut(s) 204
TaaI ACNGT 5 cut(s) 592, 1015, 1231, 1395, 1528
TaiI ACGT 6 cut(s) 480, 588, 850, 1131, 1264, 1608
TaqI TCGA 3 cut(s) 449, 679, 1512
TasI AATT 7 cut(s) 93, 856, 1082, 1095, 1378, 1425, 1485
TatI WGTACW 1 cut(s) 851
TauI GCSGC 2 cut(s) 183, 196
TfiI GAWTC 3 cut(s) 485, 1121, 1211
Tru1I TTAA 3 cut(s) 1022, 1064, 1535
Tru9I TTAA 3 cut(s) 1022, 1064, 1535
TscAI CASTG 1 cut(s) 741
TseFI GTSAC 1 cut(s) 161
TseI GCWGC 2 cut(s) 110, 137
Tsp45I GTSAC 1 cut(s) 161
TspRI CASTG 1 cut(s) 741
Tth111I GACNNNGTC 1 cut(s) 320
Van91I CCANNNNNTGG 1 cut(s) 158
VneI GTGCAC 1 cut(s) 257
VpaK11BI GGWCC 3 cut(s) 230, 401, 1564
XagI CCTNNNNNAGG 1 cut(s) 635
XapI RAATTY 3 cut(s) 93, 1378, 1425
XcmI CCANNNNNNNNNTGG 1 cut(s) 607
XmiI GTMKAC 1 cut(s) 925
XmnI GAANNNNTTC 2 cut(s) 626, 675
XspI CTAG 5 cut(s) 642, 803, 1043, 1310, 1460
ZrmI AGTACT 1 cut(s) 853
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.