Belongs to the cytochrome P450 family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 968 |
| Acc36I | ACCTGC | 2 cut(s) | 127, 968 |
| AccB1I | GGYRCC | 2 cut(s) | 620, 1241 |
| AciI | CCGC | 4 cut(s) | 165, 244, 658, 1481 |
| AclI | AACGTT | 1 cut(s) | 610 |
| AclWI | GGATC | 1 cut(s) | 532 |
| AcoI | YGGCCR | 3 cut(s) | 131, 676, 1339 |
| AcsI | RAATTY | 2 cut(s) | 505, 922 |
| AcuI | CTGAAG | 2 cut(s) | 882, 884 |
| AfaI | GTAC | 3 cut(s) | 187, 212, 837 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 140, 1184, 1234 |
| AflIII | ACRYGT | 1 cut(s) | 460 |
| AgsI | TTSAA | 7 cut(s) | 226, 298, 1033, 1136, 1274, 1313, 1475 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 1509 |
| AjiI | CACGTC | 1 cut(s) | 533 |
| AjnI | CCWGG | 4 cut(s) | 963, 1405, 1513, 1570 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 1467, 1499 |
| AleI | CACNNNNGTG | 1 cut(s) | 329 |
| Alw21I | GWGCWC | 2 cut(s) | 552, 1077 |
| Alw26I | GTCTC | 3 cut(s) | 162, 900, 1417 |
| AlwI | GGATC | 1 cut(s) | 532 |
| AoxI | GGCC | 6 cut(s) | 108, 131, 142, 676, 1175, 1339 |
| ApaI | GGGCCC | 1 cut(s) | 1179 |
| ApeKI | GCWGC | 6 cut(s) | 80, 240, 264, 335, 721, 1156 |
| ApoI | RAATTY | 2 cut(s) | 505, 922 |
| AspLEI | GCGC | 1 cut(s) | 721 |
| AspS9I | GGNCC | 6 cut(s) | 109, 514, 1175, 1176, 1187, 1279 |
| AsuC2I | CCSGG | 2 cut(s) | 487, 1235 |
| AsuHPI | GGTGA | 3 cut(s) | 271, 1427, 1549 |
| AsuII | TTCGAA | 1 cut(s) | 73 |
| AvaII | GGWCC | 3 cut(s) | 514, 1187, 1279 |
| BaeGI | GKGCMC | 2 cut(s) | 1179, 1406 |
| BalI | TGGCCA | 3 cut(s) | 133, 678, 1341 |
| BanI | GGYRCC | 2 cut(s) | 620, 1241 |
| BanII | GRGCYC | 3 cut(s) | 552, 1077, 1179 |
| BbsI | GAAGAC | 3 cut(s) | 779, 863, 1212 |
| Bbv12I | GWGCWC | 2 cut(s) | 552, 1077 |
| BbvCI | CCTCAGC | 1 cut(s) | 625 |
| BbvI | GCAGC | 6 cut(s) | 67, 252, 276, 322, 708, 1143 |
| BccI | CCATC | 4 cut(s) | 589, 635, 1148, 1309 |
| BceAI | ACGGC | 5 cut(s) | 229, 231, 347, 709, 792 |
| BciT130I | CCWGG | 4 cut(s) | 965, 1407, 1515, 1572 |
| BcnI | CCSGG | 2 cut(s) | 487, 1235 |
| BcoDI | GTCTC | 3 cut(s) | 162, 900, 1417 |
| BfaI | CTAG | 5 cut(s) | 102, 158, 236, 563, 1202 |
| BfmI | CTRYAG | 1 cut(s) | 382 |
| BfuAI | ACCTGC | 2 cut(s) | 127, 968 |
| BisI | GCNGC | 7 cut(s) | 81, 241, 244, 265, 336, 722, 1157 |
| BlpI | GCTNAGC | 1 cut(s) | 217 |
| BlsI | GCNGC | 7 cut(s) | 82, 242, 245, 266, 337, 723, 1158 |
| BmcAI | AGTACT | 1 cut(s) | 837 |
| Bme1390I | CCNGG | 6 cut(s) | 487, 965, 1235, 1407, 1515, 1572 |
| Bme18I | GGWCC | 3 cut(s) | 514, 1187, 1279 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 1509 |
| BmgBI | CACGTC | 1 cut(s) | 533 |
| BmgT120I | GGNCC | 6 cut(s) | 109, 514, 1175, 1176, 1187, 1279 |
| BmiI | GGNNCC | 9 cut(s) | 94, 111, 515, 622, 1177, 1178, 1243, 1280, 1281 |
| BmrFI | CCNGG | 6 cut(s) | 487, 965, 1235, 1407, 1515, 1572 |
| BmsI | GCATC | 6 cut(s) | 441, 706, 781, 829, 957, 1365 |
| BpiI | GAAGAC | 3 cut(s) | 779, 863, 1212 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 416, 448 |
| Bpu10I | CCTNAGC | 1 cut(s) | 625 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 217 |
| Bpu14I | TTCGAA | 1 cut(s) | 73 |
| BpuEI | CTTGAG | 1 cut(s) | 467 |
| BpuMI | CCSGG | 2 cut(s) | 487, 1235 |
| BsaBI | GATNNNNATC | 1 cut(s) | 819 |
| BsaI | GGTCTC | 1 cut(s) | 162 |
| BsaJI | CCNNGG | 5 cut(s) | 327, 537, 976, 1336, 1405 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 140, 1184, 1234 |
| Bse118I | RCCGGY | 1 cut(s) | 1220 |
| Bse1I | ACTGG | 1 cut(s) | 679 |
| Bse3DI | GCAATG | 2 cut(s) | 204, 771 |
| Bse8I | GATNNNNATC | 1 cut(s) | 819 |
| BseBI | CCWGG | 4 cut(s) | 965, 1407, 1515, 1572 |
| BseDI | CCNNGG | 5 cut(s) | 327, 537, 976, 1336, 1405 |
| BseGI | GGATG | 4 cut(s) | 600, 721, 796, 820 |
| BseJI | GATNNNNATC | 1 cut(s) | 819 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 140, 1184, 1234 |
| BseMI | GCAATG | 2 cut(s) | 204, 771 |
| BseMII | CTCAG | 3 cut(s) | 231, 639, 1497 |
| BseNI | ACTGG | 1 cut(s) | 679 |
| BseSI | GKGCMC | 2 cut(s) | 1179, 1406 |
| BseXI | GCAGC | 6 cut(s) | 67, 252, 276, 322, 708, 1143 |
| BseYI | CCCAGC | 4 cut(s) | 267, 602, 1168, 1172 |
| BsgI | GTGCAG | 1 cut(s) | 1195 |
| BshFI | GGCC | 6 cut(s) | 110, 133, 144, 678, 1177, 1341 |
| BshNI | GGYRCC | 2 cut(s) | 620, 1241 |
| BsiHKAI | GWGCWC | 2 cut(s) | 552, 1077 |
| BsiSI | CCGG | 3 cut(s) | 486, 1221, 1235 |
| BsiWI | CGTACG | 1 cut(s) | 185 |
| BslFI | GGGAC | 3 cut(s) | 519, 1217, 1292 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 140, 1184, 1234 |
| BsmAI | GTCTC | 3 cut(s) | 162, 900, 1417 |
| BsmFI | GGGAC | 3 cut(s) | 519, 1217, 1292 |
| BsnI | GGCC | 6 cut(s) | 110, 133, 144, 678, 1177, 1341 |
| Bso31I | GGTCTC | 1 cut(s) | 162 |
| Bsp119I | TTCGAA | 1 cut(s) | 73 |
| Bsp120I | GGGCCC | 1 cut(s) | 1175 |
| Bsp1286I | GDGCHC | 4 cut(s) | 552, 1077, 1179, 1406 |
| Bsp143I | GATC | 1 cut(s) | 524 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 217 |
| Bsp19I | CCATGG | 1 cut(s) | 1336 |
| BspACI | CCGC | 4 cut(s) | 165, 244, 658, 1481 |
| BspANI | GGCC | 6 cut(s) | 110, 133, 144, 678, 1177, 1341 |
| BspCNI | CTCAG | 3 cut(s) | 230, 638, 1498 |
| BspHI | TCATGA | 1 cut(s) | 844 |
| BspLI | GGNNCC | 9 cut(s) | 94, 111, 515, 622, 1177, 1178, 1243, 1280, 1281 |
| BspMI | ACCTGC | 2 cut(s) | 127, 968 |
| BspPI | GGATC | 1 cut(s) | 532 |
| BspT104I | TTCGAA | 1 cut(s) | 73 |
| BspT107I | GGYRCC | 2 cut(s) | 620, 1241 |
| BspTNI | GGTCTC | 1 cut(s) | 162 |
| BsrDI | GCAATG | 2 cut(s) | 204, 771 |
| BsrFI | RCCGGY | 1 cut(s) | 1220 |
| BsrI | ACTGG | 1 cut(s) | 679 |
| BssAI | RCCGGY | 1 cut(s) | 1220 |
| BssECI | CCNNGG | 5 cut(s) | 327, 537, 976, 1336, 1405 |
| BssMI | GATC | 1 cut(s) | 524 |
| BssT1I | CCWWGG | 2 cut(s) | 976, 1336 |
| Bst2UI | CCWGG | 4 cut(s) | 965, 1407, 1515, 1572 |
| Bst4CI | ACNGT | 5 cut(s) | 185, 331, 440, 1216, 1365 |
| Bst6I | CTCTTC | 2 cut(s) | 903, 1059 |
| BstBI | TTCGAA | 1 cut(s) | 73 |
| BstC8I | GCNNGC | 4 cut(s) | 1000, 1222, 1240, 1446 |
| BstDEI | CTNAG | 4 cut(s) | 217, 625, 1183, 1506 |
| BstDSI | CCRYGG | 3 cut(s) | 327, 537, 1336 |
| BstF5I | GGATG | 4 cut(s) | 600, 721, 796, 820 |
| BstHHI | GCGC | 1 cut(s) | 721 |
| BstKTI | GATC | 1 cut(s) | 527 |
| BstMAI | GTCTC | 3 cut(s) | 162, 900, 1417 |
| BstMBI | GATC | 1 cut(s) | 524 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 451, 817 |
| BstNI | CCWGG | 4 cut(s) | 965, 1407, 1515, 1572 |
| BstNSI | RCATGY | 1 cut(s) | 464 |
| BstSCI | CCNGG | 6 cut(s) | 485, 963, 1233, 1405, 1513, 1570 |
| BstSFI | CTRYAG | 1 cut(s) | 382 |
| BstSLI | GKGCMC | 2 cut(s) | 1179, 1406 |
| BstV1I | GCAGC | 6 cut(s) | 67, 252, 276, 322, 708, 1143 |
| BstV2I | GAAGAC | 3 cut(s) | 779, 863, 1212 |
| BstXI | CCANNNNNNTGG | 5 cut(s) | 331, 346, 964, 1252, 1290 |
| BsuRI | GGCC | 6 cut(s) | 110, 133, 144, 678, 1177, 1341 |
| BtgI | CCRYGG | 3 cut(s) | 327, 537, 1336 |
| BtgZI | GCGATG | 2 cut(s) | 660, 806 |
| BtrI | CACGTC | 1 cut(s) | 533 |
| BtsCI | GGATG | 4 cut(s) | 600, 721, 796, 820 |
| BtsIMutI | CAGTG | 1 cut(s) | 37 |
| BveI | ACCTGC | 2 cut(s) | 127, 968 |
| Cac8I | GCNNGC | 4 cut(s) | 1000, 1222, 1240, 1446 |
| CciI | TCATGA | 1 cut(s) | 844 |
| CfoI | GCGC | 1 cut(s) | 721 |
| Cfr10I | RCCGGY | 1 cut(s) | 1220 |
| Cfr13I | GGNCC | 6 cut(s) | 109, 514, 1175, 1176, 1187, 1279 |
| Csp6I | GTAC | 3 cut(s) | 186, 211, 836 |
| CviQI | GTAC | 3 cut(s) | 186, 211, 836 |
| DdeI | CTNAG | 4 cut(s) | 217, 625, 1183, 1506 |
| DpnI | GATC | 1 cut(s) | 526 |
| DpnII | GATC | 1 cut(s) | 524 |
| DriI | GACNNNNNGTC | 1 cut(s) | 1509 |
| EaeI | YGGCCR | 3 cut(s) | 131, 676, 1339 |
| Eam1104I | CTCTTC | 2 cut(s) | 903, 1059 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 1509 |
| EarI | CTCTTC | 2 cut(s) | 903, 1059 |
| EciI | GGCGGA | 1 cut(s) | 180 |
| Ecl136II | GAGCTC | 2 cut(s) | 550, 1075 |
| Eco130I | CCWWGG | 2 cut(s) | 976, 1336 |
| Eco24I | GRGCYC | 3 cut(s) | 552, 1077, 1179 |
| Eco31I | GGTCTC | 1 cut(s) | 162 |
| Eco47I | GGWCC | 3 cut(s) | 514, 1187, 1279 |
| Eco53kI | GAGCTC | 2 cut(s) | 550, 1075 |
| Eco57I | CTGAAG | 2 cut(s) | 882, 884 |
| EcoICRI | GAGCTC | 2 cut(s) | 550, 1075 |
| EcoO109I | RGGNCCY | 3 cut(s) | 109, 1176, 1279 |
| EcoRII | CCWGG | 4 cut(s) | 963, 1405, 1513, 1570 |
| EcoT14I | CCWWGG | 2 cut(s) | 976, 1336 |
| EcoT22I | ATGCAT | 1 cut(s) | 1465 |
| EcoT38I | GRGCYC | 3 cut(s) | 552, 1077, 1179 |
| ErhI | CCWWGG | 2 cut(s) | 976, 1336 |
| FaqI | GGGAC | 3 cut(s) | 519, 1217, 1292 |
| FauI | CCCGC | 1 cut(s) | 1488 |
| Fnu4HI | GCNGC | 7 cut(s) | 81, 241, 244, 265, 336, 722, 1157 |
| FokI | GGATG | 4 cut(s) | 607, 728, 803, 827 |
| FriOI | GRGCYC | 3 cut(s) | 552, 1077, 1179 |
| Fsp4HI | GCNGC | 7 cut(s) | 81, 241, 244, 265, 336, 722, 1157 |
| FspBI | CTAG | 5 cut(s) | 102, 158, 236, 563, 1202 |
| GlaI | GCGC | 1 cut(s) | 720 |
| GluI | GCNGC | 7 cut(s) | 81, 241, 244, 265, 336, 722, 1157 |
| GsaI | CCCAGC | 4 cut(s) | 271, 606, 1172, 1176 |
| HaeIII | GGCC | 6 cut(s) | 110, 133, 144, 678, 1177, 1341 |
| HapII | CCGG | 3 cut(s) | 486, 1221, 1235 |
| HhaI | GCGC | 1 cut(s) | 721 |
| Hin6I | GCGC | 1 cut(s) | 719 |
| HinP1I | GCGC | 1 cut(s) | 719 |
| HincII | GTYRAC | 3 cut(s) | 1102, 1361, 1490 |
| HindII | GTYRAC | 3 cut(s) | 1102, 1361, 1490 |
| HinfI | GANTC | 5 cut(s) | 4, 1106, 1270, 1510, 1537 |
| HpaII | CCGG | 3 cut(s) | 486, 1221, 1235 |
| HphI | GGTGA | 3 cut(s) | 271, 1427, 1549 |
| Hpy166II | GTNNAC | 5 cut(s) | 957, 1102, 1258, 1361, 1490 |
| Hpy188I | TCNGA | 8 cut(s) | 475, 666, 693, 715, 803, 940, 1111, 1530 |
| Hpy188III | TCNNGA | 9 cut(s) | 102, 318, 466, 592, 845, 902, 1030, 1202, 1327 |
| Hpy8I | GTNNAC | 5 cut(s) | 957, 1102, 1258, 1361, 1490 |
| HpyAV | CCTTC | 9 cut(s) | 68, 164, 661, 754, 902, 1048, 1389, 1442, 1577 |
| HpyCH4III | ACNGT | 5 cut(s) | 185, 331, 440, 1216, 1365 |
| HpyCH4IV | ACGT | 3 cut(s) | 532, 610, 1260 |
| HpyCH4V | TGCA | 7 cut(s) | 65, 128, 416, 748, 1212, 1426, 1463 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 451, 817 |
| HpyF3I | CTNAG | 4 cut(s) | 217, 625, 1183, 1506 |
| HpySE526I | ACGT | 3 cut(s) | 532, 610, 1260 |
| HspAI | GCGC | 1 cut(s) | 719 |
| KflI | GGGWCCC | 1 cut(s) | 1279 |
| KroI | GCCGGC | 1 cut(s) | 1220 |
| KroNI | GCCGGC | 1 cut(s) | 1222 |
| Kzo9I | GATC | 1 cut(s) | 524 |
| LmnI | GCTCC | 2 cut(s) | 194, 1080 |
| Lsp1109I | GCAGC | 6 cut(s) | 67, 252, 276, 322, 708, 1143 |
| LweI | GCATC | 6 cut(s) | 441, 706, 781, 829, 957, 1365 |
| MaeI | CTAG | 5 cut(s) | 102, 158, 236, 563, 1202 |
| MaeII | ACGT | 3 cut(s) | 532, 610, 1260 |
| MaeIII | GTNAC | 4 cut(s) | 528, 611, 635, 986 |
| MalI | GATC | 1 cut(s) | 526 |
| MboI | GATC | 1 cut(s) | 524 |
| MfeI | CAATTG | 1 cut(s) | 672 |
| MhlI | GDGCHC | 4 cut(s) | 552, 1077, 1179, 1406 |
| MlsI | TGGCCA | 3 cut(s) | 133, 678, 1341 |
| MluCI | AATT | 6 cut(s) | 48, 135, 505, 569, 672, 922 |
| MluNI | TGGCCA | 3 cut(s) | 133, 678, 1341 |
| MlyI | GAGTC | 2 cut(s) | 1519, 1531 |
| MmeI | TCCRAC | 2 cut(s) | 720, 1063 |
| Mox20I | TGGCCA | 3 cut(s) | 133, 678, 1341 |
| Mph1103I | ATGCAT | 1 cut(s) | 1465 |
| MroNI | GCCGGC | 1 cut(s) | 1220 |
| MscI | TGGCCA | 3 cut(s) | 133, 678, 1341 |
| MseI | TTAA | 2 cut(s) | 20, 579 |
| MslI | CAYNNNNRTG | 4 cut(s) | 32, 329, 1341, 1347 |
| Msp20I | TGGCCA | 3 cut(s) | 133, 678, 1341 |
| MspA1I | CMGCKG | 2 cut(s) | 267, 1172 |
| MspI | CCGG | 3 cut(s) | 486, 1221, 1235 |
| MspR9I | CCNGG | 6 cut(s) | 487, 965, 1235, 1407, 1515, 1572 |
| MunI | CAATTG | 1 cut(s) | 672 |
| MvaI | CCWGG | 4 cut(s) | 965, 1407, 1515, 1572 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 451, 817 |
| NaeI | GCCGGC | 1 cut(s) | 1222 |
| NciI | CCSGG | 2 cut(s) | 487, 1235 |
| NcoI | CCATGG | 1 cut(s) | 1336 |
| NdeII | GATC | 1 cut(s) | 524 |
| NgoMIV | GCCGGC | 1 cut(s) | 1220 |
| NlaIV | GGNNCC | 9 cut(s) | 94, 111, 515, 622, 1177, 1178, 1243, 1280, 1281 |
| NmuCI | GTSAC | 3 cut(s) | 528, 635, 986 |
| NsiI | ATGCAT | 1 cut(s) | 1465 |
| NspI | RCATGY | 1 cut(s) | 464 |
| NspV | TTCGAA | 1 cut(s) | 73 |
| OliI | CACNNNNGTG | 1 cut(s) | 329 |
| PagI | TCATGA | 1 cut(s) | 844 |
| PaqCI | CACCTGC | 1 cut(s) | 968 |
| PciI | ACATGT | 1 cut(s) | 460 |
| PdiI | GCCGGC | 1 cut(s) | 1222 |
| PfeI | GAWTC | 3 cut(s) | 4, 1106, 1270 |
| Pfl23II | CGTACG | 1 cut(s) | 185 |
| PkrI | GCNGC | 7 cut(s) | 82, 242, 245, 266, 337, 723, 1158 |
| PleI | GAGTC | 2 cut(s) | 1518, 1531 |
| PpsI | GAGTC | 2 cut(s) | 1518, 1531 |
| PpuMI | RGGWCCY | 1 cut(s) | 1279 |
| PscI | ACATGT | 1 cut(s) | 460 |
| Psp124BI | GAGCTC | 2 cut(s) | 552, 1077 |
| Psp1406I | AACGTT | 1 cut(s) | 610 |
| Psp5II | RGGWCCY | 1 cut(s) | 1279 |
| Psp6I | CCWGG | 4 cut(s) | 963, 1405, 1513, 1570 |
| PspFI | CCCAGC | 4 cut(s) | 267, 602, 1168, 1172 |
| PspGI | CCWGG | 4 cut(s) | 963, 1405, 1513, 1570 |
| PspLI | CGTACG | 1 cut(s) | 185 |
| PspN4I | GGNNCC | 9 cut(s) | 94, 111, 515, 622, 1177, 1178, 1243, 1280, 1281 |
| PspOMI | GGGCCC | 1 cut(s) | 1175 |
| PspPI | GGNCC | 6 cut(s) | 109, 514, 1175, 1176, 1187, 1279 |
| PspPPI | RGGWCCY | 1 cut(s) | 1279 |
| PvuII | CAGCTG | 2 cut(s) | 267, 1172 |
| RsaI | GTAC | 3 cut(s) | 187, 212, 837 |
| RsaNI | GTAC | 3 cut(s) | 186, 211, 836 |
| RseI | CAYNNNNRTG | 4 cut(s) | 32, 329, 1341, 1347 |
| SacI | GAGCTC | 2 cut(s) | 552, 1077 |
| SaqAI | TTAA | 2 cut(s) | 20, 579 |
| SatI | GCNGC | 7 cut(s) | 81, 241, 244, 265, 336, 722, 1157 |
| Sau3AI | GATC | 1 cut(s) | 524 |
| Sau96I | GGNCC | 6 cut(s) | 109, 514, 1175, 1176, 1187, 1279 |
| ScaI | AGTACT | 1 cut(s) | 837 |
| SchI | GAGTC | 2 cut(s) | 1519, 1531 |
| ScrFI | CCNGG | 6 cut(s) | 487, 965, 1235, 1407, 1515, 1572 |
| SduI | GDGCHC | 4 cut(s) | 552, 1077, 1179, 1406 |
| SfaNI | GCATC | 6 cut(s) | 441, 706, 781, 829, 957, 1365 |
| SfcI | CTRYAG | 1 cut(s) | 382 |
| SfuI | TTCGAA | 1 cut(s) | 73 |
| SinI | GGWCC | 3 cut(s) | 514, 1187, 1279 |
| SmiMI | CAYNNNNRTG | 4 cut(s) | 32, 329, 1341, 1347 |
| SmlI | CTYRAG | 1 cut(s) | 446 |
| SmoI | CTYRAG | 1 cut(s) | 446 |
| Sse9I | AATT | 6 cut(s) | 48, 135, 505, 569, 672, 922 |
| SsiI | CCGC | 4 cut(s) | 165, 244, 658, 1481 |
| SspMI | CTAG | 5 cut(s) | 102, 158, 236, 563, 1202 |
| SstI | GAGCTC | 2 cut(s) | 552, 1077 |
| StyD4I | CCNGG | 6 cut(s) | 485, 963, 1233, 1405, 1513, 1570 |
| StyI | CCWWGG | 2 cut(s) | 976, 1336 |
| TaaI | ACNGT | 5 cut(s) | 185, 331, 440, 1216, 1365 |
| TaiI | ACGT | 3 cut(s) | 535, 613, 1263 |
| TaqI | TCGA | 2 cut(s) | 73, 319 |
| TasI | AATT | 6 cut(s) | 48, 135, 505, 569, 672, 922 |
| TatI | WGTACW | 1 cut(s) | 835 |
| TauI | GCSGC | 1 cut(s) | 246 |
| TfiI | GAWTC | 3 cut(s) | 4, 1106, 1270 |
| Tru1I | TTAA | 2 cut(s) | 20, 579 |
| Tru9I | TTAA | 2 cut(s) | 20, 579 |
| TscAI | CASTG | 1 cut(s) | 37 |
| TseFI | GTSAC | 3 cut(s) | 528, 635, 986 |
| TseI | GCWGC | 6 cut(s) | 80, 240, 264, 335, 721, 1156 |
| Tsp45I | GTSAC | 3 cut(s) | 528, 635, 986 |
| TspDTI | ATGAA | 7 cut(s) | 17, 677, 782, 861, 868, 1064, 1448 |
| TspGWI | ACGGA | 1 cut(s) | 1507 |
| TspRI | CASTG | 1 cut(s) | 37 |
| VpaK11BI | GGWCC | 3 cut(s) | 514, 1187, 1279 |
| XapI | RAATTY | 2 cut(s) | 505, 922 |
| XbaI | TCTAGA | 2 cut(s) | 101, 1201 |
| XceI | RCATGY | 1 cut(s) | 464 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 158 |
| XspI | CTAG | 5 cut(s) | 102, 158, 236, 563, 1202 |
| ZrmI | AGTACT | 1 cut(s) | 837 |
| Zsp2I | ATGCAT | 1 cut(s) | 1465 |