Rroxscaffold_4G00296860

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000137
Physical Location & Seq
Reverse (-)
16874446 .. 16876724
2279 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00296860.1

Sequence Viewer

Length: 1590 bp
ATGGAGTTTCTGCTTCCATCTTCCGTTTGTGCTCTAACTTCCTTCTTAGCATTGGCGCTTTTTCTTTTCTCTTTTGTATGGATATCAAAATACTCGCAGAAAAACACAGCAAAAAGTAGAGGTCCCCCGGAAGCGGCTGGTGCATGGCCAATACTCGGCCATCTTCCCCTCTTAGGAGGGCCTCAAACGCCCCACATAACCCTAGGGAACATGGCTGACAAGTACGGACCGCTCTTCACCATCAAGCTTGGTGTGCACCGAGCTCTTGTTGTAAGCAGTTGGGATGTGGCTAAAGAATGTCTCACCACTAATGATAAAGCTTTTGCCAACCGCCCGAAAGCTTTATACTCAGAGATTATGGGGTACGACTATGCCATGTTCGGTCTTGGCCCTTACGGACCTTACTGGCGCCAGATGCGAAAGATAACCACGCTGCACATCCTCTCCAACCACAAGTTGGAGTTGCTCAAGCACGTCCGAGAATCGGAGGTGAAGGTCTCTACCAAACATATATTTGATATGTGGGAGCGAAACAAAGGTGTCTCAAATAAGGTGCTGGTGGACTTGAAGAGGTGGTTTGCAGACTTAACTTTAAATGTCATGTTCAGGATGGTTATTGGAAAGCGACTTCTTGGGGCTACCACGGCGCAAGAGAAGGAAGAGAACGAAAGCTTTCAAAAGGTGTTGAGGGAGTTTTTTATATTAGGTGGTCAATTTGTAATCTCAGATGCAGTTCCATATCTTAGGTGGTTGGATTTGGGAGGTTATGAGAAGGCCATGAAGAAGACGGCTAAAGAACTCGATGGTGCGGCTCAAAAATGGTTAGAAGAGCATAAGCACAAGAGAAGTACTAATTCTGTGCAACATGATTGTGAGCCTGACTTCATGGACATGATGCTTTCTGTCCTTGATGATGATAGCACAGAAGAGTTTGCTGGTTCAATTACTGCTGATACAATTAACAAAGCTACATGCCTGGCGGTTCTTCTAGCAGGGACAGACACCTCCTCAGTGACATTAACCTGGGCTCTTTCTTTATTACTCAACAATCCTGAAGTCCTGAAGAAGGCTCAACTTGAGGAAAGGCAAGTGAATGAATCAGATGTGAAGAACCTAGTCTATCTCCAAGCCATTATCAAGGAAACATTGAGGCTATACCCTGCTGCACCTCTCTTATTACCACATGAATCCAGAGAAGACTGCACAATAGGTAACTATCATGTAAGCTCAGGCACGCGTCTTCTTATCAACCTTTCAAAGCTTCAGCGCGATCCAAATGTCTGGCTCGATCCTTGTCTATTCCAACCAGAAAGGTTTCTTACAACTCACAAGAATGTTGATGTTAGGGGCCAGAGTTTTGAATTTATACCATTTGGAAGTGGTAGACGAATATGTCCAGGAATCTCTCTCGCACTTCACGTTACTCAACTTGCACTGGCTCATTTGCTACATGGGTTTGAAATTGCAACTCCATCTGATGAACCAGTTGATATGGGTGAGAGTTCAGGACTAACAAACATGAAGACCACCCCACTGGAGGTCCTTCTCAACCCACGCCTACATCCTAAACTATATGAAAATGTTGACTAG

Protein Analysis

529

Amino Acids

59.52

Weight (kDa)

6.8

Isoelectric Point (pI)

37.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 47 - 505 6.4e-88 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000137)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G31940 AT4G31950 AT4G31970
fragaria_vesca FvH4_1g17460 FvH4_2g40550 FvH4_2g40551 FvH4_2g40560 FvH4_2g40570 FvH4_2g40580 FvH4_2g40590 FvH4_7g16520 FvH4_7g16601 FvH4_7g16602 FvH4_7g22620 FvH4_7g22810
malus_domestica MD00G1037400.v1.1 MD00G1037500.v1.1 MD00G1175000.v1.1 MD00G1175100.v1.1 MD00G1175200.v1.1 MD03G1091600.v1.1 MD04G1043800.v1.1 MD04G1043900.v1.1 MD04G1044100.v1.1 MD04G1044200.v1.1 MD04G1044300.v1.1 MD04G1044400.v1.1 MD08G1234700.v1.1 MD14G1047100.v1.1 MD14G1163500.v1.1 MD15G1028200.v1.1 MD15G1028300.v1.1 MD15G1028400.v1.1 MD15G1028500.v1.1 MD15G1028700.v1.1 MD15G1032900.v1.1 MD15G1033100.v1.1 MD15G1033400.v1.1 MD15G1033800.v1.1 MD15G1033900.v1.1 MD15G1378500.v1.1 MD15G1378800.v1.1
prunus_persica Prupe.1G386800_v2.0.a1 Prupe.1G386800_v2.0.a1 Prupe.1G386900_v2.0.a1 Prupe.1G387000_v2.0.a1 Prupe.1G387100_v2.0.a1 Prupe.1G387200_v2.0.a1 Prupe.1G387300_v2.0.a1 Prupe.1G387400_v2.0.a1 Prupe.1G387500_v2.0.a1 Prupe.1G387700_v2.0.a1 Prupe.1G537400_v2.0.a1 Prupe.1G537600_v2.0.a1 Prupe.1G537700_v2.0.a1 Prupe.1G538000_v2.0.a1 Prupe.1G538200_v2.0.a1 Prupe.3G064700_v2.0.a1 Prupe.3G066200_v2.0.a1 Prupe.6G212300_v2.0.a1
pyrus_communis pycom04g03800 pycom04g03820 pycom09g19430 pycom11g16760 pycom14g13610 pycom15g02430 pycom15g02460 pycom15g02470 pycom15g02480 pycom15g02520 pycom15g03050 pycom15g03060 pycom15g03070 pycom15g03090 pycom15g03100 pycom15g03110 pycom15g03120 pycom15g33920
rosa_chinensis RchiOBHm_Chr1g0359661 RchiOBHm_Chr1g0359671 RchiOBHm_Chr1g0359711 RchiOBHm_Chr1g0359751 RchiOBHm_Chr1g0359791 RchiOBHm_Chr2g0107671 RchiOBHm_Chr2g0107681 RchiOBHm_Chr2g0107691 RchiOBHm_Chr5g0058861 RchiOBHm_Chr5g0058871 RchiOBHm_Chr5g0058891 RchiOBHm_Chr5g0058901 RchiOBHm_Chr5g0058921 RchiOBHm_Chr5g0058931 RchiOBHm_Chr5g0058941 RchiOBHm_Chr5g0058971 RchiOBHm_Chr5g0058991 RchiOBHm_Chr5g0059001 RchiOBHm_Chr5g0060081 RchiOBHm_Chr6g0300111 RchiOBHm_Chr6g0300121 RchiOBHm_Chr6g0300131 RchiOBHm_Chr6g0300141 RchiOBHm_Chr6g0300151 RchiOBHm_Chr6g0300161 RchiOBHm_Chr7g0223401 RchiOBHm_Chr7g0223411 RchiOBHm_Chr7g0233631
rosa_laevigata RLG00000001247 RLG00000011362 RLG00000011363 RLG00000011364 RLG00000011365 RLG00000011367 RLG00000017599 RLG00000017600 RLG00000027854 RLG00000027856 RLG00000027857 RLG00000035257 RLG00000035258 RLG00000035263 RLG00000035267 RLG00000035268 RLG00000035270 RLG00000035272 RLG00000035273 RLG00000035275 RLG00000035276 RLG00000035343
rosa_multiflora Rmu_co8382255.1_g000001 Rmu_sc0000441.1_g000120 Rmu_sc0000441.1_g000122 Rmu_sc0000493.1_g000037 Rmu_sc0000493.1_g000038 Rmu_sc0001670.1_g000042 Rmu_sc0001670.1_g000043 Rmu_sc0001670.1_g000044 Rmu_sc0002983.1_g000067 Rmu_sc0002983.1_g000074 Rmu_sc0003366.1_g000019 Rmu_sc0003494.1_g000002 Rmu_sc0003712.1_g000014 Rmu_sc0005703.1_g000003 Rmu_sc0005703.1_g000004 Rmu_sc0006429.1_g000014 Rmu_sc0006429.1_g000018 Rmu_sc0006429.1_g000019 Rmu_sc0008701.1_g000007 Rmu_sc0009050.1_g000002 Rmu_sc0009050.1_g000008 Rmu_sc0014587.1_g000001 Rmu_sc0034954.1_g000001 Rmu_ssc0000371.1_g000008 Rmu_ssc0000371.1_g000009 Rmu_ssc0000371.1_g000012 Rmu_ssc0000371.1_g000025 Rmu_ssc0000371.1_g000028
rosa_roxburghii Rroxscaffold_1G00021390 Rroxscaffold_1G00021450 Rroxscaffold_1G00021460 Rroxscaffold_1G00021470 Rroxscaffold_1G00021480 Rroxscaffold_1G00021500 Rroxscaffold_2G00136030 Rroxscaffold_2G00136040 Rroxscaffold_2G00136070 Rroxscaffold_3G00227100 Rroxscaffold_4G00296830 Rroxscaffold_4G00296860 Rroxscaffold_4G00296870 Rroxscaffold_7G00168050 Rroxscaffold_7G00168060 Rroxscaffold_7G00168080 Rroxscaffold_7G00168090
rosa_rugosa Rorug01G0272400 Rorug01G0272500 Rorug01G0272600 Rorug01G0272700 Rorug02G0146800.1 Rorug03G0204200 Rorug03G0204300 Rorug05G0319800 Rorug05G0319900 Rorug06G0293800 Rorug06G0293800 Rorug06G0293800 Rorug06G0293800 Rorug07G0281800
rosa_samantha Rh5DG412300 Rh5DG412400 Rh6CG421000 Rh7DG427300
rosa_wichuraiana Rw1G025420 Rw1G025430 Rw1G025440 Rw2G015520 Rw5G036240 Rw5G036250 Rw5G036260 Rw5G036270 Rw5G036950 Rw6G035500 Rw6G035510 Rw6G035520 Rw7G036140

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1392
AccB1I GGYRCC 1 cut(s) 408
AccB7I CCANNNNNTGG 1 cut(s) 457
AccBSI CCGCTC 1 cut(s) 232
AccI GTMKAC 1 cut(s) 1384
AccII CGCG 2 cut(s) 1237, 1269
AciI CCGC 5 cut(s) 134, 230, 331, 809, 980
AclWI GGATC 2 cut(s) 1265, 1283
AcoI YGGCCR 2 cut(s) 146, 157
AcsI RAATTY 1 cut(s) 1361
AcuI CTGAAG 3 cut(s) 1074, 1082, 1247
AcyI GRCGYC 1 cut(s) 409
AfaI GTAC 3 cut(s) 224, 365, 850
AfiI CCNNNNNNNGG 7 cut(s) 133, 155, 173, 457, 484, 1066, 1537
AflIII ACRYGT 1 cut(s) 1235
AgsI TTSAA 6 cut(s) 568, 677, 942, 1257, 1361, 1460
AjiI CACGTC 1 cut(s) 475
AjnI CCWGG 3 cut(s) 975, 1022, 1396
AluBI AGCT 8 cut(s) 247, 263, 320, 341, 672, 968, 1227, 1261
AluI AGCT 8 cut(s) 247, 263, 320, 341, 672, 968, 1227, 1261
Alw21I GWGCWC 3 cut(s) 34, 258, 265
Alw26I GTCTC 3 cut(s) 305, 502, 547
Alw44I GTGCAC 1 cut(s) 254
AlwI GGATC 2 cut(s) 1265, 1283
AoxI GGCC 6 cut(s) 146, 157, 179, 388, 774, 1348
ApaLI GTGCAC 1 cut(s) 254
ApeKI GCWGC 2 cut(s) 433, 1163
ApoI RAATTY 1 cut(s) 1361
Asp700I GAANNNNTTC 2 cut(s) 672, 1314
AspA2I CCTAGG 1 cut(s) 202
AspLEI GCGC 4 cut(s) 58, 411, 649, 1269
AspS9I GGNCC 7 cut(s) 122, 179, 227, 389, 398, 1348, 1540
AsuC2I CCSGG 1 cut(s) 128
AsuHPI GGTGA 4 cut(s) 229, 295, 502, 1508
AvaII GGWCC 4 cut(s) 122, 227, 398, 1540
AvrII CCTAGG 1 cut(s) 202
BaeGI GKGCMC 1 cut(s) 258
BalI TGGCCA 1 cut(s) 148
BanI GGYRCC 1 cut(s) 408
BanII GRGCYC 2 cut(s) 265, 1030
BbsI GAAGAC 4 cut(s) 791, 1203, 1232, 1529
Bbv12I GWGCWC 3 cut(s) 34, 258, 265
BbvI GCAGC 2 cut(s) 420, 1150
BccI CCATC 6 cut(s) 25, 168, 248, 604, 797, 1480
BceAI ACGGC 2 cut(s) 660, 804
BciT130I CCWGG 3 cut(s) 977, 1024, 1398
BcnI CCSGG 1 cut(s) 128
BcoDI GTCTC 3 cut(s) 305, 502, 547
BfaI CTAG 4 cut(s) 203, 989, 1115, 1588
BfoI RGCGCY 2 cut(s) 59, 412
BisI GCNGC 4 cut(s) 135, 434, 810, 1164
BlnI CCTAGG 1 cut(s) 202
BlsI GCNGC 4 cut(s) 136, 435, 811, 1165
BmcAI AGTACT 1 cut(s) 850
Bme1390I CCNGG 4 cut(s) 128, 977, 1024, 1398
Bme18I GGWCC 4 cut(s) 122, 227, 398, 1540
BmgBI CACGTC 1 cut(s) 475
BmgT120I GGNCC 7 cut(s) 122, 179, 227, 389, 398, 1348, 1540
BmiI GGNNCC 3 cut(s) 124, 410, 1349
BmrFI CCNGG 4 cut(s) 128, 977, 1024, 1398
BmsI GCATC 3 cut(s) 405, 718, 885
BpiI GAAGAC 4 cut(s) 791, 1203, 1232, 1529
BpmI CTGGAG 1 cut(s) 1556
Bpu10I CCTNAGC 1 cut(s) 1228
BpuEI CTTGAG 2 cut(s) 452, 1097
BpuMI CCSGG 1 cut(s) 128
BsaHI GRCGYC 1 cut(s) 409
BsaI GGTCTC 1 cut(s) 502
BsaJI CCNNGG 4 cut(s) 126, 202, 642, 1023
BsaXI ACNNNNNCTCC 2 cut(s) 428, 458
Bsc4I CCNNNNNNNGG 7 cut(s) 133, 155, 173, 457, 484, 1066, 1537
Bse1I ACTGG 4 cut(s) 410, 1440, 1484, 1539
BseBI CCWGG 3 cut(s) 977, 1024, 1398
BseDI CCNNGG 4 cut(s) 126, 202, 642, 1023
BseGI GGATG 4 cut(s) 289, 438, 615, 1561
BseLI CCNNNNNNNGG 7 cut(s) 133, 155, 173, 457, 484, 1066, 1537
BseMII CTCAG 4 cut(s) 363, 738, 1023, 1242
BseNI ACTGG 4 cut(s) 410, 1440, 1484, 1539
BseRI GAGGAG 1 cut(s) 997
BseSI GKGCMC 1 cut(s) 258
BseXI GCAGC 2 cut(s) 420, 1150
BsgI GTGCAG 3 cut(s) 419, 1149, 1186
Bsh1236I CGCG 2 cut(s) 1237, 1269
BshFI GGCC 6 cut(s) 148, 159, 181, 390, 776, 1350
BshNI GGYRCC 1 cut(s) 408
BsiHKAI GWGCWC 3 cut(s) 34, 258, 265
BsiSI CCGG 1 cut(s) 128
BslFI GGGAC 2 cut(s) 108, 1009
BslI CCNNNNNNNGG 7 cut(s) 133, 155, 173, 457, 484, 1066, 1537
BsmAI GTCTC 3 cut(s) 305, 502, 547
BsmFI GGGAC 2 cut(s) 108, 1009
BsnI GGCC 6 cut(s) 148, 159, 181, 390, 776, 1350
Bso31I GGTCTC 1 cut(s) 502
Bsp1286I GDGCHC 4 cut(s) 34, 258, 265, 1030
Bsp143I GATC 2 cut(s) 1270, 1288
BspACI CCGC 5 cut(s) 134, 230, 331, 809, 980
BspANI GGCC 6 cut(s) 148, 159, 181, 390, 776, 1350
BspCNI CTCAG 4 cut(s) 362, 737, 1022, 1241
BspFNI CGCG 2 cut(s) 1237, 1269
BspLI GGNNCC 3 cut(s) 124, 410, 1349
BspPI GGATC 2 cut(s) 1265, 1283
BspQI GCTCTTC 2 cut(s) 239, 822
BspT107I GGYRCC 1 cut(s) 408
BspTNI GGTCTC 1 cut(s) 502
BsrBI CCGCTC 1 cut(s) 232
BsrI ACTGG 4 cut(s) 410, 1440, 1484, 1539
BssECI CCNNGG 4 cut(s) 126, 202, 642, 1023
BssMI GATC 2 cut(s) 1270, 1288
BssNI GRCGYC 1 cut(s) 409
BssT1I CCWWGG 1 cut(s) 202
Bst2UI CCWGG 3 cut(s) 977, 1024, 1398
Bst6I CTCTTC 5 cut(s) 239, 563, 654, 822, 921
BstACI GRCGYC 1 cut(s) 409
BstC8I GCNNGC 1 cut(s) 1235
BstDEI CTNAG 7 cut(s) 46, 172, 349, 724, 743, 1009, 1228
BstDSI CCRYGG 1 cut(s) 642
BstENI CCTNNNNNAGG 1 cut(s) 1064
BstF5I GGATG 4 cut(s) 289, 438, 615, 1561
BstFNI CGCG 2 cut(s) 1237, 1269
BstH2I RGCGCY 2 cut(s) 59, 412
BstHHI GCGC 4 cut(s) 58, 411, 649, 1269
BstKTI GATC 2 cut(s) 1273, 1291
BstMAI GTCTC 3 cut(s) 305, 502, 547
BstMBI GATC 2 cut(s) 1270, 1288
BstMWI GCNNNNNNNGC 5 cut(s) 140, 187, 253, 415, 644
BstNI CCWGG 3 cut(s) 977, 1024, 1398
BstNSI RCATGY 1 cut(s) 975
BstSCI CCNGG 4 cut(s) 126, 975, 1022, 1396
BstSLI GKGCMC 1 cut(s) 258
BstUI CGCG 2 cut(s) 1237, 1269
BstV1I GCAGC 2 cut(s) 420, 1150
BstV2I GAAGAC 4 cut(s) 791, 1203, 1232, 1529
BstXI CCANNNNNNTGG 2 cut(s) 1281, 1534
BsuRI GGCC 6 cut(s) 148, 159, 181, 390, 776, 1350
BtgI CCRYGG 1 cut(s) 642
BtrI CACGTC 1 cut(s) 475
BtsCI GGATG 4 cut(s) 289, 438, 615, 1561
BtsIMutI CAGTG 3 cut(s) 1017, 1433, 1532
Cac8I GCNNGC 1 cut(s) 1235
CfoI GCGC 4 cut(s) 58, 411, 649, 1269
Cfr13I GGNCC 7 cut(s) 122, 179, 227, 389, 398, 1348, 1540
CpoI CGGWCCG 1 cut(s) 227
CseI GACGC 1 cut(s) 1226
Csp6I GTAC 3 cut(s) 223, 364, 849
CspI CGGWCCG 1 cut(s) 227
CviQI GTAC 3 cut(s) 223, 364, 849
DdeI CTNAG 7 cut(s) 46, 172, 349, 724, 743, 1009, 1228
DinI GGCGCC 1 cut(s) 410
DpnI GATC 2 cut(s) 1272, 1290
DpnII GATC 2 cut(s) 1270, 1288
DraI TTTAAA 1 cut(s) 594
DrdI GACNNNNNNGTC 1 cut(s) 1392
DseDI GACNNNNNNGTC 1 cut(s) 1392
EaeI YGGCCR 2 cut(s) 146, 157
Eam1104I CTCTTC 5 cut(s) 239, 563, 654, 822, 921
EarI CTCTTC 5 cut(s) 239, 563, 654, 822, 921
Ecl136II GAGCTC 1 cut(s) 263
Eco130I CCWWGG 1 cut(s) 202
Eco24I GRGCYC 2 cut(s) 265, 1030
Eco31I GGTCTC 1 cut(s) 502
Eco32I GATATC 1 cut(s) 84
Eco47I GGWCC 4 cut(s) 122, 227, 398, 1540
Eco53kI GAGCTC 1 cut(s) 263
Eco57I CTGAAG 3 cut(s) 1074, 1082, 1247
EcoICRI GAGCTC 1 cut(s) 263
EcoNI CCTNNNNNAGG 1 cut(s) 1064
EcoO109I RGGNCCY 3 cut(s) 122, 179, 1540
EcoRII CCWGG 3 cut(s) 975, 1022, 1396
EcoRV GATATC 1 cut(s) 84
EcoT14I CCWWGG 1 cut(s) 202
EcoT38I GRGCYC 2 cut(s) 265, 1030
EgeI GGCGCC 1 cut(s) 410
EheI GGCGCC 1 cut(s) 410
ErhI CCWWGG 1 cut(s) 202
FaqI GGGAC 2 cut(s) 108, 1009
FblI GTMKAC 1 cut(s) 1384
Fnu4HI GCNGC 4 cut(s) 135, 434, 810, 1164
FokI GGATG 4 cut(s) 296, 425, 622, 1548
FriOI GRGCYC 2 cut(s) 265, 1030
Fsp4HI GCNGC 4 cut(s) 135, 434, 810, 1164
FspBI CTAG 4 cut(s) 203, 989, 1115, 1588
GlaI GCGC 4 cut(s) 57, 410, 648, 1268
GluI GCNGC 4 cut(s) 135, 434, 810, 1164
GsuI CTGGAG 1 cut(s) 1556
HaeII RGCGCY 2 cut(s) 59, 412
HaeIII GGCC 6 cut(s) 148, 159, 181, 390, 776, 1350
HapII CCGG 1 cut(s) 128
HgaI GACGC 1 cut(s) 1226
HhaI GCGC 4 cut(s) 58, 411, 649, 1269
Hin1I GRCGYC 1 cut(s) 409
Hin6I GCGC 4 cut(s) 56, 409, 647, 1267
HinP1I GCGC 4 cut(s) 56, 409, 647, 1267
HincII GTYRAC 1 cut(s) 1585
HindII GTYRAC 1 cut(s) 1585
HindIII AAGCTT 5 cut(s) 245, 318, 339, 670, 1259
HinfI GANTC 4 cut(s) 482, 1097, 1187, 1401
HpaII CCGG 1 cut(s) 128
HphI GGTGA 4 cut(s) 229, 295, 502, 1508
Hpy166II GTNNAC 4 cut(s) 256, 562, 1385, 1585
Hpy188I TCNGA 6 cut(s) 352, 479, 487, 727, 1102, 1477
Hpy188III TCNNGA 5 cut(s) 607, 1052, 1060, 1191, 1506
Hpy8I GTNNAC 4 cut(s) 256, 562, 1385, 1585
HpyAV CCTTC 6 cut(s) 52, 487, 649, 766, 1060, 1553
HpyCH4IV ACGT 2 cut(s) 474, 1419
HpyF10VI GCNNNNNNNGC 5 cut(s) 140, 187, 253, 415, 644
HpyF3I CTNAG 7 cut(s) 46, 172, 349, 724, 743, 1009, 1228
HpySE526I ACGT 2 cut(s) 474, 1419
Hsp92I GRCGYC 1 cut(s) 409
HspAI GCGC 4 cut(s) 56, 409, 647, 1267
KasI GGCGCC 1 cut(s) 408
Kzo9I GATC 2 cut(s) 1270, 1288
LguI GCTCTTC 2 cut(s) 239, 822
LmnI GCTCC 1 cut(s) 526
Lsp1109I GCAGC 2 cut(s) 420, 1150
LweI GCATC 3 cut(s) 405, 718, 885
MaeI CTAG 4 cut(s) 203, 989, 1115, 1588
MaeII ACGT 2 cut(s) 474, 1419
MaeIII GTNAC 3 cut(s) 1012, 1211, 1420
MalI GATC 2 cut(s) 1272, 1290
MbiI CCGCTC 1 cut(s) 232
MboI GATC 2 cut(s) 1270, 1288
MhlI GDGCHC 4 cut(s) 34, 258, 265, 1030
MlsI TGGCCA 1 cut(s) 148
MluCI AATT 6 cut(s) 713, 853, 942, 957, 1361, 1461
MluI ACGCGT 1 cut(s) 1235
MluNI TGGCCA 1 cut(s) 148
Mly113I GGCGCC 1 cut(s) 409
MmeI TCCRAC 4 cut(s) 438, 471, 732, 1327
Mox20I TGGCCA 1 cut(s) 148
MroXI GAANNNNTTC 2 cut(s) 672, 1314
MscI TGGCCA 1 cut(s) 148
MseI TTAA 4 cut(s) 587, 593, 960, 1019
MslI CAYNNNNRTG 3 cut(s) 870, 890, 1332
Msp20I TGGCCA 1 cut(s) 148
MspI CCGG 1 cut(s) 128
MspR9I CCNGG 4 cut(s) 128, 977, 1024, 1398
MvaI CCWGG 3 cut(s) 977, 1024, 1398
MvnI CGCG 2 cut(s) 1237, 1269
MwoI GCNNNNNNNGC 5 cut(s) 140, 187, 253, 415, 644
NarI GGCGCC 1 cut(s) 409
NciI CCSGG 1 cut(s) 128
NdeII GATC 2 cut(s) 1270, 1288
NlaIV GGNNCC 3 cut(s) 124, 410, 1349
NmeAIII GCCGAG 1 cut(s) 135
NmuCI GTSAC 1 cut(s) 1012
NspI RCATGY 1 cut(s) 975
PciSI GCTCTTC 2 cut(s) 239, 822
PcsI WCGNNNNNNNCGW 1 cut(s) 1416
PdmI GAANNNNTTC 2 cut(s) 672, 1314
PfeI GAWTC 4 cut(s) 482, 1097, 1187, 1401
PflMI CCANNNNNTGG 1 cut(s) 457
PfoI TCCNGGA 1 cut(s) 1396
PkrI GCNGC 4 cut(s) 136, 435, 811, 1165
PluTI GGCGCC 1 cut(s) 412
PpuMI RGGWCCY 2 cut(s) 122, 1540
Psp124BI GAGCTC 1 cut(s) 265
Psp5II RGGWCCY 2 cut(s) 122, 1540
Psp6I CCWGG 3 cut(s) 975, 1022, 1396
PspGI CCWGG 3 cut(s) 975, 1022, 1396
PspN4I GGNNCC 3 cut(s) 124, 410, 1349
PspPI GGNCC 7 cut(s) 122, 179, 227, 389, 398, 1348, 1540
PspPPI RGGWCCY 2 cut(s) 122, 1540
RsaI GTAC 3 cut(s) 224, 365, 850
RsaNI GTAC 3 cut(s) 223, 364, 849
RseI CAYNNNNRTG 3 cut(s) 870, 890, 1332
Rsr2I CGGWCCG 1 cut(s) 227
RsrII CGGWCCG 1 cut(s) 227
SacI GAGCTC 1 cut(s) 265
SapI GCTCTTC 2 cut(s) 239, 822
SaqAI TTAA 4 cut(s) 587, 593, 960, 1019
SatI GCNGC 4 cut(s) 135, 434, 810, 1164
Sau3AI GATC 2 cut(s) 1270, 1288
Sau96I GGNCC 7 cut(s) 122, 179, 227, 389, 398, 1348, 1540
ScaI AGTACT 1 cut(s) 850
ScrFI CCNGG 4 cut(s) 128, 977, 1024, 1398
SduI GDGCHC 4 cut(s) 34, 258, 265, 1030
SfaNI GCATC 3 cut(s) 405, 718, 885
SfoI GGCGCC 1 cut(s) 410
SinI GGWCC 4 cut(s) 122, 227, 398, 1540
SmiMI CAYNNNNRTG 3 cut(s) 870, 890, 1332
SmlI CTYRAG 2 cut(s) 467, 1076
SmoI CTYRAG 2 cut(s) 467, 1076
Sse9I AATT 6 cut(s) 713, 853, 942, 957, 1361, 1461
SsiI CCGC 5 cut(s) 134, 230, 331, 809, 980
SspDI GGCGCC 1 cut(s) 408
SspMI CTAG 4 cut(s) 203, 989, 1115, 1588
SstI GAGCTC 1 cut(s) 265
StyD4I CCNGG 4 cut(s) 126, 975, 1022, 1396
StyI CCWWGG 1 cut(s) 202
TaiI ACGT 2 cut(s) 477, 1422
TaqI TCGA 2 cut(s) 801, 1287
TaqII GACCGA 1 cut(s) 371
TasI AATT 6 cut(s) 713, 853, 942, 957, 1361, 1461
TatI WGTACW 1 cut(s) 848
TauI GCSGC 2 cut(s) 137, 812
TfiI GAWTC 4 cut(s) 482, 1097, 1187, 1401
Tru1I TTAA 4 cut(s) 587, 593, 960, 1019
Tru9I TTAA 4 cut(s) 587, 593, 960, 1019
TscAI CASTG 3 cut(s) 1017, 1440, 1539
TseFI GTSAC 1 cut(s) 1012
TseI GCWGC 2 cut(s) 433, 1163
Tsp45I GTSAC 1 cut(s) 1012
TspDTI ATGAA 7 cut(s) 794, 874, 1110, 1200, 1494, 1535, 1590
TspGWI ACGGA 3 cut(s) 13, 240, 411
TspRI CASTG 3 cut(s) 1017, 1440, 1539
Van91I CCANNNNNTGG 1 cut(s) 457
VneI GTGCAC 1 cut(s) 254
VpaK11BI GGWCC 4 cut(s) 122, 227, 398, 1540
XagI CCTNNNNNAGG 1 cut(s) 1064
XapI RAATTY 1 cut(s) 1361
XceI RCATGY 1 cut(s) 975
XcmI CCANNNNNNNNNTGG 2 cut(s) 454, 744
XmaJI CCTAGG 1 cut(s) 202
XmiI GTMKAC 1 cut(s) 1384
XmnI GAANNNNTTC 2 cut(s) 672, 1314
XspI CTAG 4 cut(s) 203, 989, 1115, 1588
ZrmI AGTACT 1 cut(s) 850
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.