Cytochrome p450
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 1203 |
| AccB1I | GGYRCC | 1 cut(s) | 198 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 247 |
| AccBSI | CCGCTC | 1 cut(s) | 22 |
| AccI | GTMKAC | 1 cut(s) | 1195 |
| AccII | CGCG | 2 cut(s) | 1048, 1080 |
| AciI | CCGC | 4 cut(s) | 20, 121, 770, 961 |
| AclI | AACGTT | 1 cut(s) | 1062 |
| AclWI | GGATC | 2 cut(s) | 1076, 1094 |
| AcsI | RAATTY | 2 cut(s) | 1172, 1327 |
| AcuI | CTGAAG | 3 cut(s) | 864, 872, 1058 |
| AcyI | GRCGYC | 1 cut(s) | 199 |
| AfaI | GTAC | 3 cut(s) | 14, 155, 640 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 247, 274, 856, 1348 |
| AflIII | ACRYGT | 1 cut(s) | 1046 |
| AgsI | TTSAA | 7 cut(s) | 358, 467, 732, 1068, 1172, 1271, 1333 |
| AjiI | CACGTC | 1 cut(s) | 265 |
| AjnI | CCWGG | 3 cut(s) | 765, 812, 1207 |
| AluBI | AGCT | 7 cut(s) | 53, 110, 131, 462, 758, 1038, 1072 |
| AluI | AGCT | 7 cut(s) | 53, 110, 131, 462, 758, 1038, 1072 |
| Alw21I | GWGCWC | 2 cut(s) | 48, 55 |
| Alw26I | GTCTC | 3 cut(s) | 95, 292, 337 |
| Alw44I | GTGCAC | 1 cut(s) | 44 |
| AlwI | GGATC | 2 cut(s) | 1076, 1094 |
| AoxI | GGCC | 3 cut(s) | 178, 564, 1159 |
| ApaLI | GTGCAC | 1 cut(s) | 44 |
| ApeKI | GCWGC | 2 cut(s) | 223, 974 |
| ApoI | RAATTY | 2 cut(s) | 1172, 1327 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 462, 1125 |
| AspLEI | GCGC | 3 cut(s) | 201, 439, 1080 |
| AspS9I | GGNCC | 5 cut(s) | 17, 179, 188, 1159, 1351 |
| AsuHPI | GGTGA | 4 cut(s) | 19, 85, 292, 1319 |
| AvaII | GGWCC | 3 cut(s) | 17, 188, 1351 |
| BaeGI | GKGCMC | 1 cut(s) | 48 |
| BanI | GGYRCC | 1 cut(s) | 198 |
| BanII | GRGCYC | 2 cut(s) | 55, 820 |
| BbsI | GAAGAC | 4 cut(s) | 581, 1014, 1043, 1340 |
| Bbv12I | GWGCWC | 2 cut(s) | 48, 55 |
| BbvI | GCAGC | 2 cut(s) | 210, 961 |
| BccI | CCATC | 4 cut(s) | 38, 394, 587, 1291 |
| BceAI | ACGGC | 2 cut(s) | 450, 594 |
| BciT130I | CCWGG | 3 cut(s) | 767, 814, 1209 |
| BcoDI | GTCTC | 3 cut(s) | 95, 292, 337 |
| BfaI | CTAG | 3 cut(s) | 779, 926, 1399 |
| BfoI | RGCGCY | 1 cut(s) | 202 |
| BisI | GCNGC | 3 cut(s) | 224, 962, 975 |
| BlsI | GCNGC | 3 cut(s) | 225, 963, 976 |
| BmcAI | AGTACT | 1 cut(s) | 640 |
| Bme1390I | CCNGG | 3 cut(s) | 767, 814, 1209 |
| Bme18I | GGWCC | 3 cut(s) | 17, 188, 1351 |
| BmgBI | CACGTC | 1 cut(s) | 265 |
| BmgT120I | GGNCC | 5 cut(s) | 17, 179, 188, 1159, 1351 |
| BmiI | GGNNCC | 2 cut(s) | 200, 1160 |
| BmrFI | CCNGG | 3 cut(s) | 767, 814, 1209 |
| BmsI | GCATC | 3 cut(s) | 195, 508, 675 |
| BpiI | GAAGAC | 4 cut(s) | 581, 1014, 1043, 1340 |
| BpmI | CTGGAG | 1 cut(s) | 1367 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1039 |
| BpuEI | CTTGAG | 2 cut(s) | 242, 887 |
| BsaHI | GRCGYC | 1 cut(s) | 199 |
| BsaI | GGTCTC | 1 cut(s) | 292 |
| BsaJI | CCNNGG | 3 cut(s) | 37, 432, 813 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 218, 248 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 247, 274, 856, 1348 |
| Bse1I | ACTGG | 4 cut(s) | 200, 1251, 1295, 1350 |
| Bse3DI | GCAATG | 1 cut(s) | 956 |
| BseBI | CCWGG | 3 cut(s) | 767, 814, 1209 |
| BseDI | CCNNGG | 3 cut(s) | 37, 432, 813 |
| BseGI | GGATG | 4 cut(s) | 79, 228, 405, 1372 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 247, 274, 856, 1348 |
| BseMI | GCAATG | 1 cut(s) | 956 |
| BseMII | CTCAG | 4 cut(s) | 153, 528, 813, 1053 |
| BseNI | ACTGG | 4 cut(s) | 200, 1251, 1295, 1350 |
| BseRI | GAGGAG | 1 cut(s) | 787 |
| BseSI | GKGCMC | 1 cut(s) | 48 |
| BseXI | GCAGC | 2 cut(s) | 210, 961 |
| BsgI | GTGCAG | 3 cut(s) | 209, 960, 997 |
| Bsh1236I | CGCG | 2 cut(s) | 1048, 1080 |
| BshFI | GGCC | 3 cut(s) | 180, 566, 1161 |
| BshNI | GGYRCC | 1 cut(s) | 198 |
| BsiHKAI | GWGCWC | 2 cut(s) | 48, 55 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 247, 274, 856, 1348 |
| BsmAI | GTCTC | 3 cut(s) | 95, 292, 337 |
| BsnI | GGCC | 3 cut(s) | 180, 566, 1161 |
| Bso31I | GGTCTC | 1 cut(s) | 292 |
| Bsp1286I | GDGCHC | 3 cut(s) | 48, 55, 820 |
| Bsp143I | GATC | 2 cut(s) | 1081, 1099 |
| BspACI | CCGC | 4 cut(s) | 20, 121, 770, 961 |
| BspANI | GGCC | 3 cut(s) | 180, 566, 1161 |
| BspCNI | CTCAG | 4 cut(s) | 152, 527, 812, 1052 |
| BspFNI | CGCG | 2 cut(s) | 1048, 1080 |
| BspLI | GGNNCC | 2 cut(s) | 200, 1160 |
| BspPI | GGATC | 2 cut(s) | 1076, 1094 |
| BspQI | GCTCTTC | 2 cut(s) | 29, 612 |
| BspT107I | GGYRCC | 1 cut(s) | 198 |
| BspTNI | GGTCTC | 1 cut(s) | 292 |
| BsrBI | CCGCTC | 1 cut(s) | 22 |
| BsrDI | GCAATG | 1 cut(s) | 956 |
| BsrI | ACTGG | 4 cut(s) | 200, 1251, 1295, 1350 |
| BssECI | CCNNGG | 3 cut(s) | 37, 432, 813 |
| BssMI | GATC | 2 cut(s) | 1081, 1099 |
| BssNI | GRCGYC | 1 cut(s) | 199 |
| BssT1I | CCWWGG | 1 cut(s) | 37 |
| Bst2UI | CCWGG | 3 cut(s) | 767, 814, 1209 |
| Bst6I | CTCTTC | 5 cut(s) | 29, 353, 444, 612, 711 |
| BstACI | GRCGYC | 1 cut(s) | 199 |
| BstC8I | GCNNGC | 1 cut(s) | 1046 |
| BstDEI | CTNAG | 5 cut(s) | 139, 514, 533, 799, 1039 |
| BstDSI | CCRYGG | 1 cut(s) | 432 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 854 |
| BstF5I | GGATG | 4 cut(s) | 79, 228, 405, 1372 |
| BstFNI | CGCG | 2 cut(s) | 1048, 1080 |
| BstH2I | RGCGCY | 1 cut(s) | 202 |
| BstHHI | GCGC | 3 cut(s) | 201, 439, 1080 |
| BstKTI | GATC | 2 cut(s) | 1084, 1102 |
| BstMAI | GTCTC | 3 cut(s) | 95, 292, 337 |
| BstMBI | GATC | 2 cut(s) | 1081, 1099 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 205, 434 |
| BstNI | CCWGG | 3 cut(s) | 767, 814, 1209 |
| BstNSI | RCATGY | 1 cut(s) | 765 |
| BstSCI | CCNGG | 3 cut(s) | 765, 812, 1207 |
| BstSLI | GKGCMC | 1 cut(s) | 48 |
| BstUI | CGCG | 2 cut(s) | 1048, 1080 |
| BstV1I | GCAGC | 2 cut(s) | 210, 961 |
| BstV2I | GAAGAC | 4 cut(s) | 581, 1014, 1043, 1340 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 1092, 1345 |
| BsuRI | GGCC | 3 cut(s) | 180, 566, 1161 |
| BtgI | CCRYGG | 1 cut(s) | 432 |
| BtrI | CACGTC | 1 cut(s) | 265 |
| BtsCI | GGATG | 4 cut(s) | 79, 228, 405, 1372 |
| BtsIMutI | CAGTG | 3 cut(s) | 807, 1244, 1343 |
| Cac8I | GCNNGC | 1 cut(s) | 1046 |
| CfoI | GCGC | 3 cut(s) | 201, 439, 1080 |
| Cfr13I | GGNCC | 5 cut(s) | 17, 179, 188, 1159, 1351 |
| CpoI | CGGWCCG | 1 cut(s) | 17 |
| CseI | GACGC | 1 cut(s) | 1037 |
| Csp6I | GTAC | 3 cut(s) | 13, 154, 639 |
| CspI | CGGWCCG | 1 cut(s) | 17 |
| CviQI | GTAC | 3 cut(s) | 13, 154, 639 |
| DdeI | CTNAG | 5 cut(s) | 139, 514, 533, 799, 1039 |
| DinI | GGCGCC | 1 cut(s) | 200 |
| DpnI | GATC | 2 cut(s) | 1083, 1101 |
| DpnII | GATC | 2 cut(s) | 1081, 1099 |
| DraI | TTTAAA | 1 cut(s) | 384 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 1203 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 1203 |
| Eam1104I | CTCTTC | 5 cut(s) | 29, 353, 444, 612, 711 |
| EarI | CTCTTC | 5 cut(s) | 29, 353, 444, 612, 711 |
| Ecl136II | GAGCTC | 1 cut(s) | 53 |
| Eco130I | CCWWGG | 1 cut(s) | 37 |
| Eco24I | GRGCYC | 2 cut(s) | 55, 820 |
| Eco31I | GGTCTC | 1 cut(s) | 292 |
| Eco47I | GGWCC | 3 cut(s) | 17, 188, 1351 |
| Eco53kI | GAGCTC | 1 cut(s) | 53 |
| Eco57I | CTGAAG | 3 cut(s) | 864, 872, 1058 |
| EcoICRI | GAGCTC | 1 cut(s) | 53 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 854 |
| EcoO109I | RGGNCCY | 1 cut(s) | 1351 |
| EcoRII | CCWGG | 3 cut(s) | 765, 812, 1207 |
| EcoT14I | CCWWGG | 1 cut(s) | 37 |
| EcoT38I | GRGCYC | 2 cut(s) | 55, 820 |
| EgeI | GGCGCC | 1 cut(s) | 200 |
| EheI | GGCGCC | 1 cut(s) | 200 |
| ErhI | CCWWGG | 1 cut(s) | 37 |
| FblI | GTMKAC | 1 cut(s) | 1195 |
| Fnu4HI | GCNGC | 3 cut(s) | 224, 962, 975 |
| FokI | GGATG | 4 cut(s) | 86, 215, 412, 1359 |
| FriOI | GRGCYC | 2 cut(s) | 55, 820 |
| Fsp4HI | GCNGC | 3 cut(s) | 224, 962, 975 |
| FspBI | CTAG | 3 cut(s) | 779, 926, 1399 |
| GlaI | GCGC | 3 cut(s) | 200, 438, 1079 |
| GluI | GCNGC | 3 cut(s) | 224, 962, 975 |
| GsuI | CTGGAG | 1 cut(s) | 1367 |
| HaeII | RGCGCY | 1 cut(s) | 202 |
| HaeIII | GGCC | 3 cut(s) | 180, 566, 1161 |
| HgaI | GACGC | 1 cut(s) | 1037 |
| HhaI | GCGC | 3 cut(s) | 201, 439, 1080 |
| Hin1I | GRCGYC | 1 cut(s) | 199 |
| Hin6I | GCGC | 3 cut(s) | 199, 437, 1078 |
| HinP1I | GCGC | 3 cut(s) | 199, 437, 1078 |
| HincII | GTYRAC | 1 cut(s) | 1396 |
| HindII | GTYRAC | 1 cut(s) | 1396 |
| HindIII | AAGCTT | 4 cut(s) | 108, 129, 460, 1070 |
| HinfI | GANTC | 4 cut(s) | 272, 908, 998, 1212 |
| HphI | GGTGA | 4 cut(s) | 19, 85, 292, 1319 |
| Hpy166II | GTNNAC | 4 cut(s) | 46, 352, 1196, 1396 |
| Hpy188I | TCNGA | 7 cut(s) | 142, 269, 277, 517, 790, 913, 1288 |
| Hpy188III | TCNNGA | 5 cut(s) | 397, 842, 850, 1002, 1317 |
| Hpy8I | GTNNAC | 4 cut(s) | 46, 352, 1196, 1396 |
| HpyAV | CCTTC | 5 cut(s) | 277, 439, 556, 850, 1364 |
| HpyCH4IV | ACGT | 3 cut(s) | 264, 1062, 1230 |
| HpyCH4V | TGCA | 8 cut(s) | 46, 226, 371, 521, 977, 1014, 1244, 1277 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 205, 434 |
| HpyF3I | CTNAG | 5 cut(s) | 139, 514, 533, 799, 1039 |
| HpySE526I | ACGT | 3 cut(s) | 264, 1062, 1230 |
| Hsp92I | GRCGYC | 1 cut(s) | 199 |
| HspAI | GCGC | 3 cut(s) | 199, 437, 1078 |
| KasI | GGCGCC | 1 cut(s) | 198 |
| Kzo9I | GATC | 2 cut(s) | 1081, 1099 |
| LguI | GCTCTTC | 2 cut(s) | 29, 612 |
| LmnI | GCTCC | 1 cut(s) | 316 |
| Lsp1109I | GCAGC | 2 cut(s) | 210, 961 |
| LweI | GCATC | 3 cut(s) | 195, 508, 675 |
| MaeI | CTAG | 3 cut(s) | 779, 926, 1399 |
| MaeII | ACGT | 3 cut(s) | 264, 1062, 1230 |
| MaeIII | GTNAC | 3 cut(s) | 802, 1022, 1231 |
| MalI | GATC | 2 cut(s) | 1083, 1101 |
| MbiI | CCGCTC | 1 cut(s) | 22 |
| MboI | GATC | 2 cut(s) | 1081, 1099 |
| MhlI | GDGCHC | 3 cut(s) | 48, 55, 820 |
| MluCI | AATT | 7 cut(s) | 503, 643, 732, 747, 1172, 1272, 1327 |
| MluI | ACGCGT | 1 cut(s) | 1046 |
| Mly113I | GGCGCC | 1 cut(s) | 199 |
| MmeI | TCCRAC | 4 cut(s) | 228, 261, 522, 852 |
| MroXI | GAANNNNTTC | 2 cut(s) | 462, 1125 |
| MseI | TTAA | 4 cut(s) | 377, 383, 750, 809 |
| MslI | CAYNNNNRTG | 3 cut(s) | 660, 680, 1143 |
| MspR9I | CCNGG | 3 cut(s) | 767, 814, 1209 |
| MvaI | CCWGG | 3 cut(s) | 767, 814, 1209 |
| MvnI | CGCG | 2 cut(s) | 1048, 1080 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 205, 434 |
| NarI | GGCGCC | 1 cut(s) | 199 |
| NdeII | GATC | 2 cut(s) | 1081, 1099 |
| NlaIV | GGNNCC | 2 cut(s) | 200, 1160 |
| NmuCI | GTSAC | 1 cut(s) | 802 |
| NspI | RCATGY | 1 cut(s) | 765 |
| PciSI | GCTCTTC | 2 cut(s) | 29, 612 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 1227 |
| PdmI | GAANNNNTTC | 2 cut(s) | 462, 1125 |
| PfeI | GAWTC | 4 cut(s) | 272, 908, 998, 1212 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 247 |
| PfoI | TCCNGGA | 1 cut(s) | 1207 |
| PkrI | GCNGC | 3 cut(s) | 225, 963, 976 |
| PluTI | GGCGCC | 1 cut(s) | 202 |
| PpuMI | RGGWCCY | 1 cut(s) | 1351 |
| Psp124BI | GAGCTC | 1 cut(s) | 55 |
| Psp1406I | AACGTT | 1 cut(s) | 1062 |
| Psp5II | RGGWCCY | 1 cut(s) | 1351 |
| Psp6I | CCWGG | 3 cut(s) | 765, 812, 1207 |
| PspGI | CCWGG | 3 cut(s) | 765, 812, 1207 |
| PspN4I | GGNNCC | 2 cut(s) | 200, 1160 |
| PspPI | GGNCC | 5 cut(s) | 17, 179, 188, 1159, 1351 |
| PspPPI | RGGWCCY | 1 cut(s) | 1351 |
| RsaI | GTAC | 3 cut(s) | 14, 155, 640 |
| RsaNI | GTAC | 3 cut(s) | 13, 154, 639 |
| RseI | CAYNNNNRTG | 3 cut(s) | 660, 680, 1143 |
| Rsr2I | CGGWCCG | 1 cut(s) | 17 |
| RsrII | CGGWCCG | 1 cut(s) | 17 |
| SacI | GAGCTC | 1 cut(s) | 55 |
| SapI | GCTCTTC | 2 cut(s) | 29, 612 |
| SaqAI | TTAA | 4 cut(s) | 377, 383, 750, 809 |
| SatI | GCNGC | 3 cut(s) | 224, 962, 975 |
| Sau3AI | GATC | 2 cut(s) | 1081, 1099 |
| Sau96I | GGNCC | 5 cut(s) | 17, 179, 188, 1159, 1351 |
| ScaI | AGTACT | 1 cut(s) | 640 |
| ScrFI | CCNGG | 3 cut(s) | 767, 814, 1209 |
| SduI | GDGCHC | 3 cut(s) | 48, 55, 820 |
| SfaNI | GCATC | 3 cut(s) | 195, 508, 675 |
| SfoI | GGCGCC | 1 cut(s) | 200 |
| SinI | GGWCC | 3 cut(s) | 17, 188, 1351 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 660, 680, 1143 |
| SmlI | CTYRAG | 2 cut(s) | 257, 866 |
| SmoI | CTYRAG | 2 cut(s) | 257, 866 |
| Sse9I | AATT | 7 cut(s) | 503, 643, 732, 747, 1172, 1272, 1327 |
| SsiI | CCGC | 4 cut(s) | 20, 121, 770, 961 |
| SspDI | GGCGCC | 1 cut(s) | 198 |
| SspMI | CTAG | 3 cut(s) | 779, 926, 1399 |
| SstI | GAGCTC | 1 cut(s) | 55 |
| StyD4I | CCNGG | 3 cut(s) | 765, 812, 1207 |
| StyI | CCWWGG | 1 cut(s) | 37 |
| TaiI | ACGT | 3 cut(s) | 267, 1065, 1233 |
| TaqI | TCGA | 2 cut(s) | 591, 1098 |
| TaqII | GACCGA | 1 cut(s) | 161 |
| TasI | AATT | 7 cut(s) | 503, 643, 732, 747, 1172, 1272, 1327 |
| TatI | WGTACW | 1 cut(s) | 638 |
| TauI | GCSGC | 1 cut(s) | 964 |
| TfiI | GAWTC | 4 cut(s) | 272, 908, 998, 1212 |
| Tru1I | TTAA | 4 cut(s) | 377, 383, 750, 809 |
| Tru9I | TTAA | 4 cut(s) | 377, 383, 750, 809 |
| TscAI | CASTG | 3 cut(s) | 807, 1251, 1350 |
| TseFI | GTSAC | 1 cut(s) | 802 |
| TseI | GCWGC | 2 cut(s) | 223, 974 |
| Tsp45I | GTSAC | 1 cut(s) | 802 |
| TspDTI | ATGAA | 7 cut(s) | 410, 584, 664, 921, 1011, 1305, 1401 |
| TspGWI | ACGGA | 2 cut(s) | 30, 201 |
| TspRI | CASTG | 3 cut(s) | 807, 1251, 1350 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 247 |
| VneI | GTGCAC | 1 cut(s) | 44 |
| VpaK11BI | GGWCC | 3 cut(s) | 17, 188, 1351 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 854 |
| XapI | RAATTY | 2 cut(s) | 1172, 1327 |
| XceI | RCATGY | 1 cut(s) | 765 |
| XcmI | CCANNNNNNNNNTGG | 2 cut(s) | 244, 534 |
| XmiI | GTMKAC | 1 cut(s) | 1195 |
| XmnI | GAANNNNTTC | 2 cut(s) | 462, 1125 |
| XspI | CTAG | 3 cut(s) | 779, 926, 1399 |
| ZrmI | AGTACT | 1 cut(s) | 640 |