Rroxscaffold_3G00227100

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0000137
Physical Location & Seq
Forward (+)
11125784 .. 11127927
2144 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00227100.1

Sequence Viewer

Length: 1626 bp
ATGGAGATGGAGTACTCCCATCTCTTACCTGTTGTTTTGGCCTTTTCAATCATATTTCTCTACTATCTGATACTAAAACACTCCTCAAGGGTAGCGAGAAAACTCAACCTTCCACCGAAAGCCGGCGGCGCTAGGCCCATAATCGGCCACCTCCACTTATTGAGAGGCTCATCAGAACCAGCCCACATAGTGTTGGGTAAGATGGCCGATCAGTACGGACCCATCTTCACTATGAAGATGGGGATGAAGAGAACAATAGTCGTCAGCAGCTCGGAAATGGCCAAGGAGTGTTTAAAGACAAATGACGTCATCTTGGCCGGCCGTCCGAAGAGTCTAGCCAGAGAAACCCTCGGCTACGACGGTGCCTTCTTTGTCTTCAGCCGTTACGACCACTACTTCCGTAAGATACGGAGGATAGTCATGAAGGAAGCCTTGTCCAGCCACCGCCTCGAAACCCTAAAACACATTCGGGAATCCGAAGTGAAGGCCGCGATAAAGGGAATCTTTGACAGAATCAGGAGGAGGGATGGTTGTGGTTCGAATTTGAAGAAGGATCCCGGGGTGGTGGTGGAGATGGAAGAATGGTTTGAGGGCATAAACGAGAACGTGATATTTCGGATGATAGTAGGGAAACGATTTTCAGAAGCTGGGAAGATCTTAAATTCTGAGAAAGGTGATTTGATTTTGAGGGAGAAGATTAGGGGATATGTGAAATTGCTTGCAGCTTTTGCAGTGTCCGATACGATTCCATTCTTGAGGTGGTTGGACTTGTTTAGGGTTAAGAGAGAGATGAAGAAGATGGCTAAAGAGGTGGACGTTCTGCTTGAAGGATGGTTGGAAGAGCATAAGCAGAAGAGGAAGATGAATAACTCAAAGGGTGATGGTGATGATAATGAGGAGAGAGATTTCATGGATGCGATGATTTCAATTCTAGATGGTGATGACAATGTTACTTCTACTTATGATGCTGATACTATCAATAAAGCAGTCTCCTTGACTATTATCTTAGGAGGTATAGACACTATAACCGGGACATTGACATGGATTCTGACTTTACTTCTCAACAACCGCGAAGTGCTAAAGACGGCTCAACAAGAGTTAGACCAACATGTTGGTAGAGAGAGGCAAGTCGAGGAATCAGACACTGAAAACTTGGTTTATCTCCAAGCCGTTATCAAAGAAACCCTGCGCTTATACCCTCCTGGACCAATCCCAAGGCCTGATCACGAGTCCACGGAAGACTGCGTCGTCGGCGGCTACCATATCCCAGCAAAAACTCGCCTCGTGATAAATATTTCCAAGATTCAACGTGATCCGAAGGTGTGGCCTGACCCGAATGAGTTTCGACCTGAGAGGTTTCTCACAACTCACAAGAGCGTTGATGTTAGAGGTCAGGATTTTGAGTTGATACCCTTCAGCAGTGGTAGAAGAATTTGTCCTGGAATGTATCTTGCCCTAAAGAGTATGCCTCTTCTACTCGCTTCTTTGCTTCACGGTTTTGAACTTTTGACCCTAGGGGATGAGCCGGTTGACATGAGTGAGGCAGGGGGACTGACCAACCATAAAGCTGGCAAAGTTGAAGTCTTTCTCACTCCACGCCTTCCCGCTCGACTCTATCAGCATTAG

Protein Analysis

541

Amino Acids

61.5

Weight (kDa)

8.83

Isoelectric Point (pI)

43.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 42 - 522 5.1e-90 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000137)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G31940 AT4G31950 AT4G31970
fragaria_vesca FvH4_1g17460 FvH4_2g40550 FvH4_2g40551 FvH4_2g40560 FvH4_2g40570 FvH4_2g40580 FvH4_2g40590 FvH4_7g16520 FvH4_7g16601 FvH4_7g16602 FvH4_7g22620 FvH4_7g22810
malus_domestica MD00G1037400.v1.1 MD00G1037500.v1.1 MD00G1175000.v1.1 MD00G1175100.v1.1 MD00G1175200.v1.1 MD03G1091600.v1.1 MD04G1043800.v1.1 MD04G1043900.v1.1 MD04G1044100.v1.1 MD04G1044200.v1.1 MD04G1044300.v1.1 MD04G1044400.v1.1 MD08G1234700.v1.1 MD14G1047100.v1.1 MD14G1163500.v1.1 MD15G1028200.v1.1 MD15G1028300.v1.1 MD15G1028400.v1.1 MD15G1028500.v1.1 MD15G1028700.v1.1 MD15G1032900.v1.1 MD15G1033100.v1.1 MD15G1033400.v1.1 MD15G1033800.v1.1 MD15G1033900.v1.1 MD15G1378500.v1.1 MD15G1378800.v1.1
prunus_persica Prupe.1G386800_v2.0.a1 Prupe.1G386800_v2.0.a1 Prupe.1G386900_v2.0.a1 Prupe.1G387000_v2.0.a1 Prupe.1G387100_v2.0.a1 Prupe.1G387200_v2.0.a1 Prupe.1G387300_v2.0.a1 Prupe.1G387400_v2.0.a1 Prupe.1G387500_v2.0.a1 Prupe.1G387700_v2.0.a1 Prupe.1G537400_v2.0.a1 Prupe.1G537600_v2.0.a1 Prupe.1G537700_v2.0.a1 Prupe.1G538000_v2.0.a1 Prupe.1G538200_v2.0.a1 Prupe.3G064700_v2.0.a1 Prupe.3G066200_v2.0.a1 Prupe.6G212300_v2.0.a1
pyrus_communis pycom04g03800 pycom04g03820 pycom09g19430 pycom11g16760 pycom14g13610 pycom15g02430 pycom15g02460 pycom15g02470 pycom15g02480 pycom15g02520 pycom15g03050 pycom15g03060 pycom15g03070 pycom15g03090 pycom15g03100 pycom15g03110 pycom15g03120 pycom15g33920
rosa_chinensis RchiOBHm_Chr1g0359661 RchiOBHm_Chr1g0359671 RchiOBHm_Chr1g0359711 RchiOBHm_Chr1g0359751 RchiOBHm_Chr1g0359791 RchiOBHm_Chr2g0107671 RchiOBHm_Chr2g0107681 RchiOBHm_Chr2g0107691 RchiOBHm_Chr5g0058861 RchiOBHm_Chr5g0058871 RchiOBHm_Chr5g0058891 RchiOBHm_Chr5g0058901 RchiOBHm_Chr5g0058921 RchiOBHm_Chr5g0058931 RchiOBHm_Chr5g0058941 RchiOBHm_Chr5g0058971 RchiOBHm_Chr5g0058991 RchiOBHm_Chr5g0059001 RchiOBHm_Chr5g0060081 RchiOBHm_Chr6g0300111 RchiOBHm_Chr6g0300121 RchiOBHm_Chr6g0300131 RchiOBHm_Chr6g0300141 RchiOBHm_Chr6g0300151 RchiOBHm_Chr6g0300161 RchiOBHm_Chr7g0223401 RchiOBHm_Chr7g0223411 RchiOBHm_Chr7g0233631
rosa_laevigata RLG00000001247 RLG00000011362 RLG00000011363 RLG00000011364 RLG00000011365 RLG00000011367 RLG00000017599 RLG00000017600 RLG00000027854 RLG00000027856 RLG00000027857 RLG00000035257 RLG00000035258 RLG00000035263 RLG00000035267 RLG00000035268 RLG00000035270 RLG00000035272 RLG00000035273 RLG00000035275 RLG00000035276 RLG00000035343
rosa_multiflora Rmu_co8382255.1_g000001 Rmu_sc0000441.1_g000120 Rmu_sc0000441.1_g000122 Rmu_sc0000493.1_g000037 Rmu_sc0000493.1_g000038 Rmu_sc0001670.1_g000042 Rmu_sc0001670.1_g000043 Rmu_sc0001670.1_g000044 Rmu_sc0002983.1_g000067 Rmu_sc0002983.1_g000074 Rmu_sc0003366.1_g000019 Rmu_sc0003494.1_g000002 Rmu_sc0003712.1_g000014 Rmu_sc0005703.1_g000003 Rmu_sc0005703.1_g000004 Rmu_sc0006429.1_g000014 Rmu_sc0006429.1_g000018 Rmu_sc0006429.1_g000019 Rmu_sc0008701.1_g000007 Rmu_sc0009050.1_g000002 Rmu_sc0009050.1_g000008 Rmu_sc0014587.1_g000001 Rmu_sc0034954.1_g000001 Rmu_ssc0000371.1_g000008 Rmu_ssc0000371.1_g000009 Rmu_ssc0000371.1_g000012 Rmu_ssc0000371.1_g000025 Rmu_ssc0000371.1_g000028
rosa_roxburghii Rroxscaffold_1G00021390 Rroxscaffold_1G00021450 Rroxscaffold_1G00021460 Rroxscaffold_1G00021470 Rroxscaffold_1G00021480 Rroxscaffold_1G00021500 Rroxscaffold_2G00136030 Rroxscaffold_2G00136040 Rroxscaffold_2G00136070 Rroxscaffold_3G00227100 Rroxscaffold_4G00296830 Rroxscaffold_4G00296860 Rroxscaffold_4G00296870 Rroxscaffold_7G00168050 Rroxscaffold_7G00168060 Rroxscaffold_7G00168080 Rroxscaffold_7G00168090
rosa_rugosa Rorug01G0272400 Rorug01G0272500 Rorug01G0272600 Rorug01G0272700 Rorug02G0146800.1 Rorug03G0204200 Rorug03G0204300 Rorug05G0319800 Rorug05G0319900 Rorug06G0293800 Rorug06G0293800 Rorug06G0293800 Rorug06G0293800 Rorug07G0281800
rosa_samantha Rh5DG412300 Rh5DG412400 Rh6CG421000 Rh7DG427300
rosa_wichuraiana Rw1G025420 Rw1G025430 Rw1G025440 Rw2G015520 Rw5G036240 Rw5G036250 Rw5G036260 Rw5G036270 Rw5G036950 Rw6G035500 Rw6G035510 Rw6G035520 Rw7G036140

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1247
AatII GACGTC 1 cut(s) 309
AccB1I GGYRCC 1 cut(s) 362
AccBSI CCGCTC 1 cut(s) 1607
AccII CGCG 2 cut(s) 491, 1071
AciI CCGC 6 cut(s) 126, 445, 489, 1069, 1254, 1605
AclWI GGATC 3 cut(s) 548, 561, 1307
AcoI YGGCCR 5 cut(s) 145, 204, 279, 315, 319
AcsI RAATTY 3 cut(s) 541, 661, 1431
AcuI CTGAAG 2 cut(s) 361, 1399
AcyI GRCGYC 1 cut(s) 306
AdeI CACNNNGTG 1 cut(s) 190
AfaI GTAC 2 cut(s) 14, 215
AfiI CCNNNNNNNGG 2 cut(s) 122, 143
AflIII ACRYGT 1 cut(s) 1108
AgsI TTSAA 7 cut(s) 48, 547, 827, 927, 1307, 1502, 1580
AjnI CCWGG 2 cut(s) 1201, 1438
AluBI AGCT 4 cut(s) 270, 647, 725, 1568
AluI AGCT 4 cut(s) 270, 647, 725, 1568
Alw26I GTCTC 1 cut(s) 994
AlwI GGATC 3 cut(s) 548, 561, 1307
AlwNI CAGNNNCTG 2 cut(s) 647, 1145
Ama87I CYCGRG 1 cut(s) 557
ApeKI GCWGC 2 cut(s) 267, 722
ApoI RAATTY 3 cut(s) 541, 661, 1431
ArsI GACNNNNNNTTYG 2 cut(s) 1566, 1598
Asp700I GAANNNNTTC 1 cut(s) 1584
AspA2I CCTAGG 1 cut(s) 1513
AspLEI GCGC 2 cut(s) 131, 1191
AspS9I GGNCC 3 cut(s) 135, 218, 1205
AsuC2I CCSGG 3 cut(s) 558, 559, 1030
AsuHPI GGTGA 4 cut(s) 686, 890, 896, 950
AsuII TTCGAA 1 cut(s) 539
AvaI CYCGRG 1 cut(s) 557
AvaII GGWCC 2 cut(s) 218, 1205
AvrII CCTAGG 1 cut(s) 1513
BalI TGGCCA 1 cut(s) 281
BamHI GGATCC 1 cut(s) 553
BanI GGYRCC 1 cut(s) 362
BauI CACGAG 2 cut(s) 1226, 1283
BbsI GAAGAC 2 cut(s) 367, 1245
BbvI GCAGC 2 cut(s) 279, 734
BceAI ACGGC 4 cut(s) 306, 366, 1101, 1154
BciT130I CCWGG 2 cut(s) 1203, 1440
BclI TGATCA 1 cut(s) 1222
BcnI CCSGG 3 cut(s) 558, 559, 1030
BcoDI GTCTC 1 cut(s) 994
BfaI CTAG 4 cut(s) 132, 335, 932, 1514
BfoI RGCGCY 1 cut(s) 132
BglII AGATCT 1 cut(s) 654
BisI GCNGC 5 cut(s) 127, 268, 489, 723, 1255
BlnI CCTAGG 1 cut(s) 1513
BlsI GCNGC 5 cut(s) 128, 269, 490, 724, 1256
BmcAI AGTACT 1 cut(s) 14
Bme1390I CCNGG 5 cut(s) 558, 559, 1030, 1203, 1440
Bme18I GGWCC 2 cut(s) 218, 1205
BmeT110I CYCGRG 1 cut(s) 557
BmgT120I GGNCC 3 cut(s) 135, 218, 1205
BmiI GGNNCC 3 cut(s) 220, 364, 555
BmrFI CCNGG 5 cut(s) 558, 559, 1030, 1203, 1440
BmsI GCATC 2 cut(s) 904, 955
BpiI GAAGAC 2 cut(s) 367, 1245
BplI GAGNNNNNCTC 4 cut(s) 333, 365, 1453, 1485
Bpu14I TTCGAA 1 cut(s) 539
BpuEI CTTGAG 2 cut(s) 70, 775
BpuMI CCSGG 3 cut(s) 558, 559, 1030
BsaHI GRCGYC 1 cut(s) 306
BsaJI CCNNGG 7 cut(s) 282, 349, 557, 558, 1214, 1233, 1513
BsaXI ACNNNNNCTCC 3 cut(s) 26, 514, 544
Bsc4I CCNNNNNNNGG 2 cut(s) 122, 143
Bse118I RCCGGY 3 cut(s) 122, 317, 1525
BseBI CCWGG 2 cut(s) 1203, 1440
BseDI CCNNGG 7 cut(s) 282, 349, 557, 558, 1214, 1233, 1513
BseGI GGATG 6 cut(s) 249, 532, 624, 836, 919, 1525
BseLI CCNNNNNNNGG 2 cut(s) 122, 143
BseMII CTCAG 2 cut(s) 657, 1341
BseRI GAGGAG 3 cut(s) 73, 535, 911
BseX3I CGGCCG 1 cut(s) 319
BseXI GCAGC 2 cut(s) 279, 734
BseYI CCCAGC 2 cut(s) 647, 1267
Bsh1236I CGCG 2 cut(s) 491, 1071
Bsh1285I CGRYCG 1 cut(s) 322
BshNI GGYRCC 1 cut(s) 362
BsiEI CGRYCG 1 cut(s) 322
BsiHKCI CYCGRG 1 cut(s) 557
BsiSI CCGG 5 cut(s) 123, 318, 558, 1029, 1526
BslFI GGGAC 2 cut(s) 1045, 1563
BslI CCNNNNNNNGG 2 cut(s) 122, 143
BsmAI GTCTC 1 cut(s) 994
BsmFI GGGAC 2 cut(s) 1045, 1563
BsoBI CYCGRG 1 cut(s) 557
Bsp119I TTCGAA 1 cut(s) 539
Bsp143I GATC 5 cut(s) 208, 553, 654, 1222, 1312
BspACI CCGC 6 cut(s) 126, 445, 489, 1069, 1254, 1605
BspCNI CTCAG 2 cut(s) 658, 1342
BspFNI CGCG 2 cut(s) 491, 1071
BspHI TCATGA 1 cut(s) 420
BspLI GGNNCC 3 cut(s) 220, 364, 555
BspPI GGATC 3 cut(s) 548, 561, 1307
BspQI GCTCTTC 1 cut(s) 834
BspT104I TTCGAA 1 cut(s) 539
BspT107I GGYRCC 1 cut(s) 362
BsrBI CCGCTC 1 cut(s) 1607
BsrFI RCCGGY 3 cut(s) 122, 317, 1525
BssAI RCCGGY 3 cut(s) 122, 317, 1525
BssECI CCNNGG 7 cut(s) 282, 349, 557, 558, 1214, 1233, 1513
BssMI GATC 5 cut(s) 208, 553, 654, 1222, 1312
BssNI GRCGYC 1 cut(s) 306
BssSI CACGAG 2 cut(s) 1226, 1283
BssT1I CCWWGG 3 cut(s) 282, 1214, 1513
Bst2BI CACGAG 2 cut(s) 1226, 1283
Bst2UI CCWGG 2 cut(s) 1203, 1440
Bst4CI ACNGT 2 cut(s) 362, 1496
Bst6I CTCTTC 5 cut(s) 242, 323, 834, 848, 1476
BstACI GRCGYC 1 cut(s) 306
BstAPI GCANNNNNTGC 1 cut(s) 728
BstBI TTCGAA 1 cut(s) 539
BstC8I GCNNGC 4 cut(s) 124, 319, 720, 1570
BstDEI CTNAG 3 cut(s) 666, 1006, 1350
BstDSI CCRYGG 1 cut(s) 1233
BstF5I GGATG 6 cut(s) 249, 532, 624, 836, 919, 1525
BstFNI CGCG 2 cut(s) 491, 1071
BstH2I RGCGCY 1 cut(s) 132
BstHHI GCGC 2 cut(s) 131, 1191
BstKTI GATC 5 cut(s) 211, 556, 657, 1225, 1315
BstMAI GTCTC 1 cut(s) 994
BstMBI GATC 5 cut(s) 208, 553, 654, 1222, 1312
BstMCI CGRYCG 1 cut(s) 322
BstMWI GCNNNNNNNGC 3 cut(s) 128, 728, 1251
BstNI CCWGG 2 cut(s) 1203, 1440
BstNSI RCATGY 1 cut(s) 1112
BstSCI CCNGG 5 cut(s) 556, 557, 1028, 1201, 1438
BstUI CGCG 2 cut(s) 491, 1071
BstV1I GCAGC 2 cut(s) 279, 734
BstV2I GAAGAC 2 cut(s) 367, 1245
BstX2I RGATCY 2 cut(s) 553, 654
BstXI CCANNNNNNTGG 2 cut(s) 1112, 1568
BstYI RGATCY 2 cut(s) 553, 654
BstZI CGGCCG 1 cut(s) 319
BtgI CCRYGG 1 cut(s) 1233
BtgZI GCGATG 1 cut(s) 932
BtsCI GGATG 6 cut(s) 249, 532, 624, 836, 919, 1525
BtsI GCAGTG 2 cut(s) 738, 1426
BtsIMutI CAGTG 3 cut(s) 738, 1143, 1426
Cac8I GCNNGC 4 cut(s) 124, 319, 720, 1570
CaiI CAGNNNCTG 2 cut(s) 647, 1145
CciI TCATGA 1 cut(s) 420
CfoI GCGC 2 cut(s) 131, 1191
Cfr10I RCCGGY 3 cut(s) 122, 317, 1525
Cfr13I GGNCC 3 cut(s) 135, 218, 1205
Cfr9I CCCGGG 1 cut(s) 557
CseI GACGC 1 cut(s) 1234
Csp6I GTAC 2 cut(s) 13, 214
CviAII CATG 5 cut(s) 421, 910, 1041, 1109, 1534
CviQI GTAC 2 cut(s) 13, 214
DdeI CTNAG 3 cut(s) 666, 1006, 1350
DpnI GATC 5 cut(s) 210, 555, 656, 1224, 1314
DpnII GATC 5 cut(s) 208, 553, 654, 1222, 1312
DraI TTTAAA 1 cut(s) 294
DraIII CACNNNGTG 1 cut(s) 190
DrdI GACNNNNNNGTC 1 cut(s) 1247
DseDI GACNNNNNNGTC 1 cut(s) 1247
EaeI YGGCCR 5 cut(s) 145, 204, 279, 315, 319
EagI CGGCCG 1 cut(s) 319
Eam1104I CTCTTC 5 cut(s) 242, 323, 834, 848, 1476
EarI CTCTTC 5 cut(s) 242, 323, 834, 848, 1476
EclXI CGGCCG 1 cut(s) 319
Eco130I CCWWGG 3 cut(s) 282, 1214, 1513
Eco147I AGGCCT 1 cut(s) 1219
Eco47I GGWCC 2 cut(s) 218, 1205
Eco52I CGGCCG 1 cut(s) 319
Eco57I CTGAAG 2 cut(s) 361, 1399
Eco88I CYCGRG 1 cut(s) 557
EcoRII CCWGG 2 cut(s) 1201, 1438
EcoT14I CCWWGG 3 cut(s) 282, 1214, 1513
ErhI CCWWGG 3 cut(s) 282, 1214, 1513
FaeI CATG 5 cut(s) 424, 913, 1044, 1112, 1537
FalI AAGNNNNNCTT 4 cut(s) 416, 448, 488, 520
FaqI GGGAC 2 cut(s) 1045, 1563
FatI CATG 5 cut(s) 420, 909, 1040, 1108, 1533
FauI CCCGC 1 cut(s) 1612
FbaI TGATCA 1 cut(s) 1222
Fnu4HI GCNGC 5 cut(s) 127, 268, 489, 723, 1255
FokI GGATG 6 cut(s) 256, 539, 631, 843, 926, 1532
FseI GGCCGGCC 1 cut(s) 321
Fsp4HI GCNGC 5 cut(s) 127, 268, 489, 723, 1255
FspBI CTAG 4 cut(s) 132, 335, 932, 1514
GlaI GCGC 2 cut(s) 130, 1190
GluI GCNGC 5 cut(s) 127, 268, 489, 723, 1255
GsaI CCCAGC 2 cut(s) 651, 1271
HaeII RGCGCY 1 cut(s) 132
HapII CCGG 5 cut(s) 123, 318, 558, 1029, 1526
HgaI GACGC 1 cut(s) 1234
HhaI GCGC 2 cut(s) 131, 1191
Hin1I GRCGYC 1 cut(s) 306
Hin1II CATG 5 cut(s) 424, 913, 1044, 1112, 1537
Hin6I GCGC 2 cut(s) 129, 1189
HinP1I GCGC 2 cut(s) 129, 1189
HincII GTYRAC 1 cut(s) 1531
HindII GTYRAC 1 cut(s) 1531
HpaII CCGG 5 cut(s) 123, 318, 558, 1029, 1526
HphI GGTGA 4 cut(s) 686, 890, 896, 950
Hpy166II GTNNAC 3 cut(s) 814, 1233, 1531
Hpy188III TCNNGA 8 cut(s) 421, 470, 517, 754, 932, 1226, 1285, 1394
Hpy8I GTNNAC 3 cut(s) 814, 1233, 1531
Hpy99I CGWCG 3 cut(s) 362, 1250, 1253
HpyAV CCTTC 9 cut(s) 119, 376, 418, 478, 544, 821, 1312, 1423, 1610
HpyCH4III ACNGT 2 cut(s) 362, 1496
HpyCH4IV ACGT 4 cut(s) 306, 606, 816, 1309
HpyCH4V TGCA 2 cut(s) 722, 731
HpyF10VI GCNNNNNNNGC 3 cut(s) 128, 728, 1251
HpyF3I CTNAG 3 cut(s) 666, 1006, 1350
HpySE526I ACGT 4 cut(s) 306, 606, 816, 1309
Hsp92I GRCGYC 1 cut(s) 306
Hsp92II CATG 5 cut(s) 424, 913, 1044, 1112, 1537
HspAI GCGC 2 cut(s) 129, 1189
KroI GCCGGC 2 cut(s) 122, 317
KroNI GCCGGC 2 cut(s) 124, 319
Ksp22I TGATCA 1 cut(s) 1222
Kzo9I GATC 5 cut(s) 208, 553, 654, 1222, 1312
LguI GCTCTTC 1 cut(s) 834
Lsp1109I GCAGC 2 cut(s) 279, 734
LweI GCATC 2 cut(s) 904, 955
MaeI CTAG 4 cut(s) 132, 335, 932, 1514
MaeII ACGT 4 cut(s) 306, 606, 816, 1309
MaeIII GTNAC 2 cut(s) 383, 949
MalI GATC 5 cut(s) 210, 555, 656, 1224, 1314
MbiI CCGCTC 1 cut(s) 1607
MboI GATC 5 cut(s) 208, 553, 654, 1222, 1312
MflI RGATCY 2 cut(s) 553, 654
MlsI TGGCCA 1 cut(s) 281
MluCI AATT 5 cut(s) 541, 661, 713, 927, 1431
MluNI TGGCCA 1 cut(s) 281
MlyI GAGTC 3 cut(s) 340, 1238, 1605
MmeI TCCRAC 2 cut(s) 744, 816
Mox20I TGGCCA 1 cut(s) 281
MroNI GCCGGC 2 cut(s) 122, 317
MroXI GAANNNNTTC 1 cut(s) 1584
MscI TGGCCA 1 cut(s) 281
MseI TTAA 3 cut(s) 293, 659, 780
MslI CAYNNNNRTG 1 cut(s) 1039
Msp20I TGGCCA 1 cut(s) 281
MspI CCGG 5 cut(s) 123, 318, 558, 1029, 1526
MspR9I CCNGG 5 cut(s) 558, 559, 1030, 1203, 1440
MvaI CCWGG 2 cut(s) 1203, 1440
MvnI CGCG 2 cut(s) 491, 1071
MwoI GCNNNNNNNGC 3 cut(s) 128, 728, 1251
NaeI GCCGGC 2 cut(s) 124, 319
NciI CCSGG 3 cut(s) 558, 559, 1030
NdeII GATC 5 cut(s) 208, 553, 654, 1222, 1312
NgoMIV GCCGGC 2 cut(s) 122, 317
NlaIII CATG 5 cut(s) 424, 913, 1044, 1112, 1537
NlaIV GGNNCC 3 cut(s) 220, 364, 555
NmeAIII GCCGAG 1 cut(s) 330
NspI RCATGY 1 cut(s) 1112
NspV TTCGAA 1 cut(s) 539
PagI TCATGA 1 cut(s) 420
PceI AGGCCT 1 cut(s) 1219
PciI ACATGT 1 cut(s) 1108
PciSI GCTCTTC 1 cut(s) 834
PdiI GCCGGC 2 cut(s) 124, 319
PdmI GAANNNNTTC 1 cut(s) 1584
PfeI GAWTC 7 cut(s) 473, 501, 513, 745, 1045, 1136, 1303
PflFI GACNNNGTC 1 cut(s) 1244
PfoI TCCNGGA 2 cut(s) 1201, 1438
PkrI GCNGC 5 cut(s) 128, 269, 490, 724, 1256
PleI GAGTC 3 cut(s) 339, 1237, 1605
PpsI GAGTC 3 cut(s) 339, 1237, 1605
PscI ACATGT 1 cut(s) 1108
Psp6I CCWGG 2 cut(s) 1201, 1438
PspFI CCCAGC 2 cut(s) 647, 1267
PspGI CCWGG 2 cut(s) 1201, 1438
PspN4I GGNNCC 3 cut(s) 220, 364, 555
PspPI GGNCC 3 cut(s) 135, 218, 1205
PstNI CAGNNNCTG 2 cut(s) 647, 1145
PsuI RGATCY 2 cut(s) 553, 654
PsyI GACNNNGTC 1 cut(s) 1244
RigI GGCCGGCC 1 cut(s) 321
RsaI GTAC 2 cut(s) 14, 215
RsaNI GTAC 2 cut(s) 13, 214
RseI CAYNNNNRTG 1 cut(s) 1039
SapI GCTCTTC 1 cut(s) 834
SaqAI TTAA 3 cut(s) 293, 659, 780
SatI GCNGC 5 cut(s) 127, 268, 489, 723, 1255
Sau3AI GATC 5 cut(s) 208, 553, 654, 1222, 1312
Sau96I GGNCC 3 cut(s) 135, 218, 1205
ScaI AGTACT 1 cut(s) 14
SchI GAGTC 3 cut(s) 340, 1238, 1605
ScrFI CCNGG 5 cut(s) 558, 559, 1030, 1203, 1440
SfaNI GCATC 2 cut(s) 904, 955
SfuI TTCGAA 1 cut(s) 539
SinI GGWCC 2 cut(s) 218, 1205
SmaI CCCGGG 1 cut(s) 559
SmiMI CAYNNNNRTG 1 cut(s) 1039
SmlI CTYRAG 2 cut(s) 85, 754
SmoI CTYRAG 2 cut(s) 85, 754
Sse9I AATT 5 cut(s) 541, 661, 713, 927, 1431
SseBI AGGCCT 1 cut(s) 1219
SsiI CCGC 6 cut(s) 126, 445, 489, 1069, 1254, 1605
SspI AATATT 1 cut(s) 1294
SspMI CTAG 4 cut(s) 132, 335, 932, 1514
StuI AGGCCT 1 cut(s) 1219
StyD4I CCNGG 5 cut(s) 556, 557, 1028, 1201, 1438
StyI CCWWGG 3 cut(s) 282, 1214, 1513
TaaI ACNGT 2 cut(s) 362, 1496
TaiI ACGT 4 cut(s) 309, 609, 819, 1312
TaqI TCGA 5 cut(s) 450, 539, 1131, 1345, 1609
TasI AATT 5 cut(s) 541, 661, 713, 927, 1431
TatI WGTACW 1 cut(s) 12
TauI GCSGC 3 cut(s) 129, 491, 1257
TfiI GAWTC 7 cut(s) 473, 501, 513, 745, 1045, 1136, 1303
Tru1I TTAA 3 cut(s) 293, 659, 780
Tru9I TTAA 3 cut(s) 293, 659, 780
TscAI CASTG 3 cut(s) 738, 1150, 1426
TseI GCWGC 2 cut(s) 267, 722
TspDTI ATGAA 6 cut(s) 248, 260, 437, 806, 878, 898
TspGWI ACGGA 4 cut(s) 231, 389, 424, 1250
TspMI CCCGGG 1 cut(s) 557
TspRI CASTG 3 cut(s) 738, 1150, 1426
Tth111I GACNNNGTC 1 cut(s) 1244
VpaK11BI GGWCC 2 cut(s) 218, 1205
XapI RAATTY 3 cut(s) 541, 661, 1431
XbaI TCTAGA 1 cut(s) 931
XceI RCATGY 1 cut(s) 1112
XcmI CCANNNNNNNNNTGG 1 cut(s) 756
XmaI CCCGGG 1 cut(s) 557
XmaJI CCTAGG 1 cut(s) 1513
XmnI GAANNNNTTC 1 cut(s) 1584
XspI CTAG 4 cut(s) 132, 335, 932, 1514
ZraI GACGTC 1 cut(s) 307
ZrmI AGTACT 1 cut(s) 14
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.