Belongs to the protein kinase superfamily. Ser Thr protein kinase family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AatII | GACGTC | 1 cut(s) | 84 |
| AclI | AACGTT | 1 cut(s) | 123 |
| AclWI | GGATC | 2 cut(s) | 8, 45 |
| AcyI | GRCGYC | 1 cut(s) | 81 |
| AfaI | GTAC | 1 cut(s) | 283 |
| AflII | CTTAAG | 1 cut(s) | 185 |
| AflIII | ACRYGT | 1 cut(s) | 159 |
| AgsI | TTSAA | 1 cut(s) | 57 |
| AhlI | ACTAGT | 1 cut(s) | 284 |
| AluBI | AGCT | 2 cut(s) | 110, 256 |
| AluI | AGCT | 2 cut(s) | 110, 256 |
| Alw26I | GTCTC | 1 cut(s) | 38 |
| AlwI | GGATC | 2 cut(s) | 8, 45 |
| AsuHPI | GGTGA | 1 cut(s) | 207 |
| BcoDI | GTCTC | 1 cut(s) | 38 |
| BcuI | ACTAGT | 1 cut(s) | 284 |
| BfaI | CTAG | 1 cut(s) | 285 |
| BfrI | CTTAAG | 1 cut(s) | 185 |
| BmcAI | AGTACT | 1 cut(s) | 283 |
| BoxI | GACNNNNGTC | 1 cut(s) | 80 |
| BsaHI | GRCGYC | 1 cut(s) | 81 |
| BsaJI | CCNNGG | 2 cut(s) | 85, 218 |
| BseDI | CCNNGG | 2 cut(s) | 85, 218 |
| BseGI | GGATG | 1 cut(s) | 234 |
| BseMII | CTCAG | 2 cut(s) | 123, 219 |
| BseRI | GAGGAG | 1 cut(s) | 254 |
| BsmAI | GTCTC | 1 cut(s) | 38 |
| Bsp143I | GATC | 2 cut(s) | 13, 37 |
| BspCNI | CTCAG | 2 cut(s) | 124, 218 |
| BspPI | GGATC | 2 cut(s) | 8, 45 |
| BspTI | CTTAAG | 1 cut(s) | 185 |
| BssECI | CCNNGG | 2 cut(s) | 85, 218 |
| BssMI | GATC | 2 cut(s) | 13, 37 |
| BssNI | GRCGYC | 1 cut(s) | 81 |
| BssT1I | CCWWGG | 1 cut(s) | 218 |
| Bst6I | CTCTTC | 1 cut(s) | 218 |
| BstACI | GRCGYC | 1 cut(s) | 81 |
| BstAFI | CTTAAG | 1 cut(s) | 185 |
| BstC8I | GCNNGC | 2 cut(s) | 198, 202 |
| BstDEI | CTNAG | 3 cut(s) | 132, 140, 205 |
| BstDSI | CCRYGG | 1 cut(s) | 85 |
| BstF5I | GGATG | 1 cut(s) | 234 |
| BstKTI | GATC | 2 cut(s) | 16, 40 |
| BstMAI | GTCTC | 1 cut(s) | 38 |
| BstMBI | GATC | 2 cut(s) | 13, 37 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 26 |
| BstNSI | RCATGY | 1 cut(s) | 204 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 80 |
| BtgI | CCRYGG | 1 cut(s) | 85 |
| BtsCI | GGATG | 1 cut(s) | 234 |
| BtsIMutI | CAGTG | 1 cut(s) | 129 |
| Cac8I | GCNNGC | 2 cut(s) | 198, 202 |
| Csp6I | GTAC | 1 cut(s) | 282 |
| CviAII | CATG | 1 cut(s) | 201 |
| CviJI | RGCY | 3 cut(s) | 110, 196, 256 |
| CviKI_1 | RGCY | 3 cut(s) | 110, 196, 256 |
| CviQI | GTAC | 1 cut(s) | 282 |
| DdeI | CTNAG | 3 cut(s) | 132, 140, 205 |
| DpnI | GATC | 2 cut(s) | 15, 39 |
| DpnII | GATC | 2 cut(s) | 13, 37 |
| Eam1104I | CTCTTC | 1 cut(s) | 218 |
| EarI | CTCTTC | 1 cut(s) | 218 |
| Eco130I | CCWWGG | 1 cut(s) | 218 |
| EcoT14I | CCWWGG | 1 cut(s) | 218 |
| ErhI | CCWWGG | 1 cut(s) | 218 |
| FaeI | CATG | 1 cut(s) | 204 |
| FaiI | YATR | 7 cut(s) | 156, 173, 202, 236, 264, 266, 279 |
| FatI | CATG | 1 cut(s) | 200 |
| FokI | GGATG | 1 cut(s) | 241 |
| FspBI | CTAG | 1 cut(s) | 285 |
| Hin1I | GRCGYC | 1 cut(s) | 81 |
| Hin1II | CATG | 1 cut(s) | 204 |
| HincII | GTYRAC | 1 cut(s) | 127 |
| HindII | GTYRAC | 1 cut(s) | 127 |
| HinfI | GANTC | 2 cut(s) | 136, 212 |
| HphI | GGTGA | 1 cut(s) | 207 |
| Hpy166II | GTNNAC | 2 cut(s) | 127, 247 |
| Hpy188I | TCNGA | 1 cut(s) | 208 |
| Hpy188III | TCNNGA | 3 cut(s) | 35, 41, 57 |
| Hpy8I | GTNNAC | 2 cut(s) | 127, 247 |
| HpyCH4IV | ACGT | 3 cut(s) | 81, 123, 159 |
| HpyCH4V | TGCA | 3 cut(s) | 50, 171, 200 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 26 |
| HpyF3I | CTNAG | 3 cut(s) | 132, 140, 205 |
| HpySE526I | ACGT | 3 cut(s) | 81, 123, 159 |
| Hsp92I | GRCGYC | 1 cut(s) | 81 |
| Hsp92II | CATG | 1 cut(s) | 204 |
| Kzo9I | GATC | 2 cut(s) | 13, 37 |
| LmnI | GCTCC | 1 cut(s) | 253 |
| LpnPI | CCDG | 3 cut(s) | 20, 30, 235 |
| MaeI | CTAG | 1 cut(s) | 285 |
| MaeII | ACGT | 3 cut(s) | 81, 123, 159 |
| MaeIII | GTNAC | 2 cut(s) | 73, 89 |
| MalI | GATC | 2 cut(s) | 15, 39 |
| MboI | GATC | 2 cut(s) | 13, 37 |
| MboII | GAAGA | 3 cut(s) | 128, 221, 235 |
| MluCI | AATT | 1 cut(s) | 6 |
| MmeI | TCCRAC | 1 cut(s) | 203 |
| MnlI | CCTC | 3 cut(s) | 219, 232, 235 |
| MseI | TTAA | 1 cut(s) | 186 |
| MspCI | CTTAAG | 1 cut(s) | 185 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 26 |
| NdeII | GATC | 2 cut(s) | 13, 37 |
| NlaIII | CATG | 1 cut(s) | 204 |
| NmuCI | GTSAC | 1 cut(s) | 73 |
| NspI | RCATGY | 1 cut(s) | 204 |
| PaeI | GCATGC | 1 cut(s) | 204 |
| PfeI | GAWTC | 2 cut(s) | 136, 212 |
| PshAI | GACNNNNGTC | 1 cut(s) | 80 |
| Psp1406I | AACGTT | 1 cut(s) | 123 |
| RsaI | GTAC | 1 cut(s) | 283 |
| RsaNI | GTAC | 1 cut(s) | 282 |
| SaqAI | TTAA | 1 cut(s) | 186 |
| Sau3AI | GATC | 2 cut(s) | 13, 37 |
| ScaI | AGTACT | 1 cut(s) | 283 |
| SetI | ASST | 6 cut(s) | 84, 112, 126, 162, 246, 258 |
| SmlI | CTYRAG | 1 cut(s) | 185 |
| SmoI | CTYRAG | 1 cut(s) | 185 |
| SpeI | ACTAGT | 1 cut(s) | 284 |
| SphI | GCATGC | 1 cut(s) | 204 |
| Sse9I | AATT | 1 cut(s) | 6 |
| SspMI | CTAG | 1 cut(s) | 285 |
| StyI | CCWWGG | 1 cut(s) | 218 |
| TaiI | ACGT | 3 cut(s) | 84, 126, 162 |
| TasI | AATT | 1 cut(s) | 6 |
| TatI | WGTACW | 1 cut(s) | 281 |
| TfiI | GAWTC | 2 cut(s) | 136, 212 |
| Tru1I | TTAA | 1 cut(s) | 186 |
| Tru9I | TTAA | 1 cut(s) | 186 |
| TscAI | CASTG | 1 cut(s) | 136 |
| TseFI | GTSAC | 1 cut(s) | 73 |
| Tsp45I | GTSAC | 1 cut(s) | 73 |
| TspDTI | ATGAA | 3 cut(s) | 84, 129, 164 |
| TspGWI | ACGGA | 1 cut(s) | 74 |
| TspRI | CASTG | 1 cut(s) | 136 |
| Vha464I | CTTAAG | 1 cut(s) | 185 |
| XceI | RCATGY | 1 cut(s) | 204 |
| XspI | CTAG | 1 cut(s) | 285 |
| ZraI | GACGTC | 1 cut(s) | 82 |
| ZrmI | AGTACT | 1 cut(s) | 283 |