RchiOBHm_Chr1g0369841
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0000248
Physical Location & Seq
Reverse (-)
59518344 .. 59522022
3679 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ59407

Sequence Viewer

Length: 3168 bp
ATGGAGCTTCATATGCTCAACTTCTGCGCATTTCGATCTACCTACCTTCATGTCATGACCACCCTTTTCCTTCTCACCAACCTTTTCCAATCTACCATCTTTGCAAATGCATTGAGCAATGAAACCGATCGCTTGGCTTTGCTGAAATTCAAAGATTGCATAGCCACCGATCCAGATGGGCTATTGAACTCATGGAATGACTCCGTTCACTTCTGCAAATGGCAAGGAATTACTTGTGGTAGACGGCATCAAAGAGTAAAAGCCTTGAACCTACCAGACGCTGATTTGCATGGAACCATATCACCATACATTGGCAACCTCTCCTTTCTCAGGATATTCGGCCTTTTCAATAACAGCTTCTCTGGCAAGATTCCACAACAAGTTGATCATTTGTTCAGACTGCGAGGTATCAATCTAGGTATCAACAAGTTGGAGGGGGGAATTCCAGTCAACCTGACCTTCTACTCGAAATTAAGATTCATAAGCCTTGCAGCAAACCGCCTTACCGGCAAAATTCCTTCAGAGATTGGGTCATTGGGGAAGCTTGTGGATCTCATTCTAGAGGAAAACAATCTGACGGGACCCATCCCACCTTCCATGGGGAATCTTTCATCACTCACTCTACTTGACTTGGCACAGAACAATTTGGTGGGCACCGTTCCAGAGGTGCTAGGCCGATTGAGAAGCTTATCTGATTTTGCAATTGGTATCAATACTCTATCTGGTATGATCCCTCCCTCCCTTTTTAATATATCATCCATCAAGACCATCTATCTTACGGAGAATAAGTTTAAGGGCAGTATTCCGCCTGATATAGGCCTAAACATGCCTAATCTCCAACTACTGTTACTTGGTATAAATGAATTCTCTGGGAAAATCCCGGCCTCACTTTCCAATGCTTCTAAGCTTCAGAAGCTTGATATTGGGGAAAATAATTTTGTTGGGCAAGTTCCCGCAACTTTTGGAAATTTTCCTAATCTCTTGTTTCTCAACTTCTATAGCAATAATCTAGGAAGTAATTCATCAAATGATTTGGGATTTATAACATTCTTGACAAATTGCAGCAATCTGGAAATAGTTGATCTGAGTTCTAACAATTTTGGAGGTGTTTTACCAAACTCTGTAGCCAATTTCTCAACCCAACTGACTAAACTCTACCTTGGGAGGAATCAAATAGCGGGAACGATTCCTGAAACATTAGGAAATCTGAACAATTTAATACTCTTGACCCTGGAAGTAAACTTGTTCACAAGTATCATTCCGGCTTCTCTTGGGAAGTTACAAAAGCTGCAAATATTATATTTATATTCCAATAGATTATCAGGCTGGATCCCATCTTCCTTAGGAAACCTCACCCAATTGTCTGAACTCACTATATTTGAAAATGAATTAGAAGGAAGCATTCCTCCAAATATTGGTAACTGCAAAAATCTGCAGGCGATGGATATATCAGACAATAAGCTTAGTGGAGATATACCATCACAGATCATTGGTCTGTCCTCCTCTATCTTCCTCAACTTATCGCAAAACTCGCTAACTGGCATTCTGCCTGTGGAAGTGGGTAAGCTGAAGAATATCAATACACTGGACATCTCTGATAATAATTTGACTGGGGGAATTCCAGAAATTATTGGAGGATGTCTGAGCCTTGAATATCTTTACCTACAAGGGAATCTCTTCCAAGGTATGATACCTTCTTCTTTGGCTTCTTTGAGAGGTCTTCAGTCTTTAGATCTTTCACGAAGCAACTTGTCAGGACATATTCCAAAAGACCTACAGAGACTTCCATTCTTGATATATTTGAACCTTTCGTTCAATAACTTGGAGGGTGAGGTACCGAAAGAAGGAGTTTTTCGAAACACAAGTGCAGTATCATTGCATGGAAATACCAAACTTTGTGGTGGTGTTTCGGAATTGCAGCTACCAGCATGCCCCATCAAAGTACCAGAGCAGAGAAAGTTGCATGGTTTCAAACTAAAGCTCACAATTTCTTTAGTTGCTGGATGCGCTCTTCTGTTTGCAGTGATCATAGCTCTTTATTGGAGGAGAAAAACACAAAAGAATATGCCGTTATCTGCAGTGTCATCAATCAACTTCCTTCCAAAGGTTTCATACCAGACACTTCATCAAGCTACTGACGGATTCTCTCTGGGCAATCAAATTGGATCAGGTAGTTTTGGCTCTGTATACAAAGGGATTCTTGATCAACAAGAAAACAACGTTGTTGCCATAAAGGTCCTCAACCTTCAACAGAAAGGAGCTTTCAAGAGTTTTGTGGCAGAATGCAATGCACTAAGAAATATCCGGCACAGGAACCTTGTGAAGATCATAACATGCTGCTCTAGCACAGATTACAATGGTAATGACTTCAAAGCTCTAGTTTTTGAGTATATGTCAAATGGAAGTTTAGAGGAGTGGTTGCACAGAGAAAACCAATCAAGGAGTTTGAACCTCCTTCAAAGACTGAATATTGCTGTTGATGTGGCTTCTGCATTGTGTTATCTTCATGACCATTGTGAACCACAAATTATTCATCGTGACATGAAGCCGAGCAACGTTCTTCTTGACGATGACATGGTCGCTCGTGTCGGTGATTTTGGATTAGCAAGACTCATCCCACCAACCATGTACTTCTGTGAAAATCAAAGTAGCACAGTTGGGATAAAGGGAACCATTGGCTATGCTGCTCCAGAGTACGCAGTTGGTGTGGGGCCATCAAAGCAAGGGGATGTCTATAGTTATGGGATCCTTGTGTTGCAATTATTCACGGGAAGAAGACCCACTGATGAAATGTTTGTAGATGGTTGCAATATCCATACTTTTGTTAAGACGGCCATACATGGAAGACTAATGCAAATTGTAGATCCTACTCTTGTTGCCACTCTAGAAGAGATTGCAACTTCAACAACGAACAATGAAGTGACCAATATCTGTGGTTACAACAATGAAATCGAAGCTGATGAAGGCAACATTGACAATGAGAATTTAAGCACGATGAACACTTACGTGTGGAAGTGCATACTTCCAACCCTTAAGATTGGACTCGCATGCTCGGAAGAATCCCCAAGGAATAGGATATCTATGGAGGAAGTCCACAGGGAGCTACACCATATAAAAAATGCTTACACTAATGTCGACATCCGTCGAGAGAGGCCAAGAAGAAGCTAA

Protein Analysis

1055

Amino Acids

116.14

Weight (kDa)

6.85

Isoelectric Point (pI)

36.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 41 - 79 3.3e-10 Leucine rich repeat N-terminal domain
LRR_14 PF23598 102 - 336 7.4e-11 Leucine-rich repeat region
LRR_14 PF23598 380 - 461 1.1e-06 Leucine-rich repeat region
LRR_8 PF13855 381 - 440 5.9e-08 Leucine rich repeat
LRR_14 PF23598 504 - 609 1.6e-07 Leucine-rich repeat region
LRR_8 PF13855 549 - 608 2.2e-07 Leucine rich repeat
Pkinase PF00069 716 - 929 9.1e-41 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 717 - 934 3.9e-42 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000248)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g31390 FvH4_5g31630 FvH4_5g31631 FvH4_7g24151 FvH4_7g24160 FvH4_7g24240 FvH4_7g24242 FvH4_7g24243 FvH4_7g24510 FvH4_7g24920 FvH4_7g24920 FvH4_7g24920 FvH4_7g29910
malus_domestica MD01G1131800.v1.1 MD01G1150000.v1.1 MD01G1151300.v1.1
prunus_persica Prupe.2G248300_v2.0.a1
pyrus_communis pycom01g16800
rosa_chinensis RchiOBHm_Chr1g0339821 RchiOBHm_Chr1g0345151 RchiOBHm_Chr1g0346851 RchiOBHm_Chr1g0351981 RchiOBHm_Chr1g0369731 RchiOBHm_Chr1g0369841 RchiOBHm_Chr1g0369861 RchiOBHm_Chr1g0369931 RchiOBHm_Chr1g0369961 RchiOBHm_Chr1g0370261 RchiOBHm_Chr1g0370281 RchiOBHm_Chr5g0076391 RchiOBHm_Chr6g0248051 RchiOBHm_Chr6g0248071
rosa_laevigata RLG00000002548 RLG00000013479 RLG00000027042 RLG00000027045 RLG00000027047 RLG00000027060 RLG00000027063 RLG00000027082 RLG00000027084 RLG00000027087 RLG00000027089 RLG00000027090 RLG00000027091 RLG00000027092 RLG00000027094 RLG00000027097 RLG00000027100 RLG00000027101 RLG00000027111 RLG00000028769 RLG00000028851 RLG00000029244 RLG00000030021
rosa_multiflora Rmu_co7968444.1_g000001 Rmu_co8060564.1_g000001 Rmu_co8098766.1_g000001 Rmu_co8172886.1_g000001 Rmu_co8291893.1_g000001 Rmu_co8327887.1_g000001 Rmu_co8406981.1_g000001 Rmu_co8449189.1_g000001 Rmu_co8470871.1_g000001 Rmu_sc0000756.1_g000006 Rmu_sc0001154.1_g000028 Rmu_sc0001565.1_g000055 Rmu_sc0001657.1_g000002 Rmu_sc0002095.1_g000014 Rmu_sc0002147.1_g000001 Rmu_sc0002705.1_g000004 Rmu_sc0002705.1_g000005 Rmu_sc0004087.1_g000025 Rmu_sc0005500.1_g000022 Rmu_sc0006707.1_g000005 Rmu_sc0006707.1_g000009 Rmu_sc0008140.1_g000012 Rmu_sc0008140.1_g000019 Rmu_sc0016176.1_g000001 Rmu_sc0016906.1_g000006 Rmu_sc0028980.1_g000001 Rmu_sc0041369.1_g000001
rosa_roxburghii Rroxscaffold_3G00232610 Rroxscaffold_4G00286690 Rroxscaffold_4G00286920 Rroxscaffold_4G00286930 Rroxscaffold_4G00286960 Rroxscaffold_4G00286980 Rroxscaffold_4G00287030 Rroxscaffold_4G00287040 Rroxscaffold_4G00287070 Rroxscaffold_4G00287090 Rroxscaffold_4G00287330 Rroxscaffold_4G00287430 Rroxscaffold_4G00303380 Rroxscaffold_4G00303400 Rroxscaffold_4G00303410 Rroxscaffold_4G00308100 Rroxscaffold_4G00308480 Rroxscaffold_4G00313700 Rroxscaffold_4G00322910 Rroxscaffold_7G00190680 Rroxscaffold_7G00193490
rosa_rugosa Rorug01G0032200 Rorug01G0139400.1 Rorug01G0139600.1 Rorug01G0139700.1 Rorug01G0184400 Rorug01G0351900 Rorug01G0351900 Rorug01G0352600 Rorug01G0352600 Rorug01G0352800 Rorug01G0355300 Rorug01G0355400 Rorug01G0356700.1 Rorug01G0356800 Rorug06G0090600 Rorug07G0276100
rosa_samantha Rh1AG201900 Rh1AG361300 Rh1AG365100 Rh6AG204000
rosa_wichuraiana Rw0G015480 Rw1G007000 Rw1G008190 Rw1G012880 Rw1G012990 Rw1G016950 Rw1G031720 Rw1G031800 Rw1G031820 Rw1G032020 Rw1G032180 Rw6G017720 Rw7G035600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1043
Acc16I TGCGCA 1 cut(s) 28
Acc65I GGTACC 1 cut(s) 1834
AccB1I GGYRCC 2 cut(s) 653, 1834
AccB7I CCANNNNNTGG 2 cut(s) 311, 1415
AccI GTMKAC 3 cut(s) 241, 2187, 3135
AciI CCGC 4 cut(s) 499, 806, 954, 1178
AclI AACGTT 2 cut(s) 2220, 2556
AclWI GGATC 9 cut(s) 164, 558, 724, 1324, 1337, 2173, 2740, 2753, 2858
AcoI YGGCCR 1 cut(s) 2832
AcsI RAATTY 7 cut(s) 146, 441, 513, 863, 967, 1617, 2983
AcuI CTGAAG 4 cut(s) 504, 893, 1589, 1706
AfaI GTAC 4 cut(s) 1836, 1944, 2630, 2696
AfiI CCNNNNNNNGG 7 cut(s) 311, 330, 599, 815, 1415, 1844, 2104
AflII CTTAAG 1 cut(s) 3032
AflIII ACRYGT 1 cut(s) 3006
AjnI CCWGG 1 cut(s) 1230
AleI CACNNNNGTG 1 cut(s) 3005
Alw26I GTCTC 1 cut(s) 1774
AlwI GGATC 9 cut(s) 164, 558, 724, 1324, 1337, 2173, 2740, 2753, 2858
AlwNI CAGNNNCTG 1 cut(s) 281
AoxI GGCC 7 cut(s) 340, 673, 817, 882, 2711, 2832, 3152
ApeKI GCWGC 6 cut(s) 491, 1062, 1288, 1918, 2337, 2684
ApoI RAATTY 7 cut(s) 146, 441, 513, 863, 967, 1617, 2983
Asp700I GAANNNNTTC 2 cut(s) 1018, 1676
Asp718I GGTACC 1 cut(s) 1834
AspLEI GCGC 2 cut(s) 29, 2009
AspS9I GGNCC 3 cut(s) 581, 2236, 2711
AsuC2I CCSGG 1 cut(s) 881
AsuHPI GGTGA 5 cut(s) 67, 294, 1345, 1841, 2603
AsuII TTCGAA 1 cut(s) 1855
AvaII GGWCC 2 cut(s) 581, 2236
AxyI CCTNAGG 1 cut(s) 1342
BaeGI GKGCMC 1 cut(s) 656
BamHI GGATCC 2 cut(s) 1329, 2745
BanI GGYRCC 2 cut(s) 653, 1834
BarI GAAGNNNNNNTAC 2 cut(s) 1678, 1710
BauI CACGAG 1 cut(s) 2583
BbsI GAAGAC 3 cut(s) 1712, 2782, 2851
BbvI GCAGC 6 cut(s) 503, 1074, 1275, 1930, 2324, 2671
BceAI ACGGC 3 cut(s) 260, 2053, 2847
BciT130I CCWGG 1 cut(s) 1232
BclI TGATCA 3 cut(s) 385, 2025, 2203
BcnI CCSGG 1 cut(s) 881
BcoDI GTCTC 1 cut(s) 1774
BfaI CTAG 7 cut(s) 416, 560, 671, 1010, 2343, 2378, 2885
BfmI CTRYAG 6 cut(s) 997, 1122, 1433, 1775, 2076, 2734
BfrI CTTAAG 1 cut(s) 3032
BglI GCCNNNNNGGC 1 cut(s) 507
BglII AGATCT 1 cut(s) 1732
BisI GCNGC 6 cut(s) 492, 1063, 1289, 1919, 2338, 2685
BlsI GCNGC 6 cut(s) 493, 1064, 1290, 1920, 2339, 2686
Bme1390I CCNGG 2 cut(s) 881, 1232
Bme18I GGWCC 2 cut(s) 581, 2236
BmgT120I GGNCC 3 cut(s) 581, 2236, 2711
BmrFI CCNGG 2 cut(s) 881, 1232
BmrI ACTGGG 1 cut(s) 1620
BmsI GCATC 2 cut(s) 256, 1994
BmuI ACTGGG 1 cut(s) 1620
BoxI GACNNNNGTC 1 cut(s) 3141
BpiI GAAGAC 3 cut(s) 1712, 2782, 2851
BpmI CTGGAG 1 cut(s) 2673
Bpu14I TTCGAA 1 cut(s) 1855
BpuMI CCSGG 1 cut(s) 881
BsaAI YACGTR 1 cut(s) 3007
BsaJI CCNNGG 5 cut(s) 597, 1159, 1230, 1681, 3065
BsaXI ACNNNNNCTCC 2 cut(s) 773, 803
Bsc4I CCNNNNNNNGG 7 cut(s) 311, 330, 599, 815, 1415, 1844, 2104
Bse118I RCCGGY 1 cut(s) 506
Bse1I ACTGG 4 cut(s) 446, 1543, 1590, 1615
Bse21I CCTNAGG 1 cut(s) 1342
Bse3DI GCAATG 3 cut(s) 124, 1874, 2293
BseBI CCWGG 1 cut(s) 1232
BseDI CCNNGG 5 cut(s) 597, 1159, 1230, 1681, 3065
BseGI GGATG 7 cut(s) 585, 755, 1643, 2009, 2613, 2734, 3138
BseLI CCNNNNNNNGG 7 cut(s) 311, 330, 599, 815, 1415, 1844, 2104
BseMI GCAATG 3 cut(s) 124, 1874, 2293
BseMII CTCAG 3 cut(s) 343, 1076, 1634
BseNI ACTGG 4 cut(s) 446, 1543, 1590, 1615
BseRI GAGGAG 3 cut(s) 1492, 2059, 2426
BseSI GKGCMC 1 cut(s) 656
BseXI GCAGC 6 cut(s) 503, 1074, 1275, 1930, 2324, 2671
BsgI GTGCAG 1 cut(s) 1887
Bsh1285I CGRYCG 1 cut(s) 130
BshFI GGCC 7 cut(s) 342, 675, 819, 884, 2713, 2834, 3154
BshNI GGYRCC 2 cut(s) 653, 1834
BsiEI CGRYCG 1 cut(s) 130
BsiSI CCGG 4 cut(s) 507, 881, 1262, 2305
BslFI GGGAC 1 cut(s) 594
BslI CCNNNNNNNGG 7 cut(s) 311, 330, 599, 815, 1415, 1844, 2104
BsmAI GTCTC 1 cut(s) 1774
BsmFI GGGAC 1 cut(s) 594
BsmI GAATGC 3 cut(s) 1401, 1542, 2288
BsnI GGCC 7 cut(s) 342, 675, 819, 884, 2713, 2834, 3154
Bsp119I TTCGAA 1 cut(s) 1855
Bsp1286I GDGCHC 1 cut(s) 656
Bsp19I CCATGG 1 cut(s) 597
BspACI CCGC 4 cut(s) 499, 806, 954, 1178
BspANI GGCC 7 cut(s) 342, 675, 819, 884, 2713, 2834, 3154
BspCNI CTCAG 3 cut(s) 342, 1077, 1635
BspHI TCATGA 2 cut(s) 54, 2506
BspMAI CTGCAG 2 cut(s) 1437, 2080
BspPI GGATC 9 cut(s) 164, 558, 724, 1324, 1337, 2173, 2740, 2753, 2858
BspQI GCTCTTC 1 cut(s) 2016
BspT104I TTCGAA 1 cut(s) 1855
BspT107I GGYRCC 2 cut(s) 653, 1834
BspTI CTTAAG 1 cut(s) 3032
BsrDI GCAATG 3 cut(s) 124, 1874, 2293
BsrFI RCCGGY 1 cut(s) 506
BsrI ACTGG 4 cut(s) 446, 1543, 1590, 1615
BssAI RCCGGY 1 cut(s) 506
BssECI CCNNGG 5 cut(s) 597, 1159, 1230, 1681, 3065
BssNAI GTATAC 1 cut(s) 2188
BssSI CACGAG 1 cut(s) 2583
BssT1I CCWWGG 4 cut(s) 597, 1159, 1681, 3065
Bst1107I GTATAC 1 cut(s) 2188
Bst2BI CACGAG 1 cut(s) 2583
Bst2UI CCWGG 1 cut(s) 1232
Bst4CI ACNGT 3 cut(s) 658, 846, 2656
Bst6I CTCTTC 3 cut(s) 1682, 2016, 2883
BstAFI CTTAAG 1 cut(s) 3032
BstBAI YACGTR 1 cut(s) 3007
BstBI TTCGAA 1 cut(s) 1855
BstC8I GCNNGC 3 cut(s) 1437, 1930, 3049
BstDEI CTNAG 7 cut(s) 329, 903, 1085, 1342, 1463, 1643, 2294
BstDSI CCRYGG 1 cut(s) 597
BstENI CCTNNNNNAGG 3 cut(s) 328, 813, 2102
BstF5I GGATG 7 cut(s) 585, 755, 1643, 2009, 2613, 2734, 3138
BstHHI GCGC 2 cut(s) 29, 2009
BstMAI GTCTC 1 cut(s) 1774
BstMCI CGRYCG 1 cut(s) 130
BstMWI GCNNNNNNNGC 8 cut(s) 13, 363, 507, 913, 1531, 2006, 2343, 2719
BstNI CCWGG 1 cut(s) 1232
BstNSI RCATGY 4 cut(s) 829, 1932, 2337, 3051
BstPAI GACNNNNGTC 1 cut(s) 3141
BstSCI CCNGG 2 cut(s) 879, 1230
BstSFI CTRYAG 6 cut(s) 997, 1122, 1433, 1775, 2076, 2734
BstSLI GKGCMC 1 cut(s) 656
BstV1I GCAGC 6 cut(s) 503, 1074, 1275, 1930, 2324, 2671
BstV2I GAAGAC 3 cut(s) 1712, 2782, 2851
BstX2I RGATCY 5 cut(s) 550, 1329, 1732, 2745, 2863
BstYI RGATCY 5 cut(s) 550, 1329, 1732, 2745, 2863
BstZ17I GTATAC 1 cut(s) 2188
Bsu36I CCTNAGG 1 cut(s) 1342
BsuRI GGCC 7 cut(s) 342, 675, 819, 884, 2713, 2834, 3154
BtgI CCRYGG 1 cut(s) 597
BtgZI GCGATG 1 cut(s) 1454
BtsCI GGATG 7 cut(s) 585, 755, 1643, 2009, 2613, 2734, 3138
BtsI GCAGTG 2 cut(s) 2028, 2085
BtsIMutI CAGTG 4 cut(s) 1583, 2028, 2085, 2781
Cac8I GCNNGC 3 cut(s) 1437, 1930, 3049
CaiI CAGNNNCTG 1 cut(s) 281
CciI TCATGA 2 cut(s) 54, 2506
CfoI GCGC 2 cut(s) 29, 2009
Cfr10I RCCGGY 1 cut(s) 506
Cfr13I GGNCC 3 cut(s) 581, 2236, 2711
CseI GACGC 1 cut(s) 287
Csp6I GTAC 4 cut(s) 1835, 1943, 2629, 2695
CspCI CAANNNNNGTGG 4 cut(s) 1879, 1914, 2914, 2949
CviQI GTAC 4 cut(s) 1835, 1943, 2629, 2695
DdeI CTNAG 7 cut(s) 329, 903, 1085, 1342, 1463, 1643, 2294
EaeI YGGCCR 1 cut(s) 2832
Eam1104I CTCTTC 3 cut(s) 1682, 2016, 2883
EarI CTCTTC 3 cut(s) 1682, 2016, 2883
EciI GGCGGA 1 cut(s) 795
Eco130I CCWWGG 4 cut(s) 597, 1159, 1681, 3065
Eco147I AGGCCT 1 cut(s) 819
Eco32I GATATC 1 cut(s) 3078
Eco47I GGWCC 2 cut(s) 581, 2236
Eco57I CTGAAG 4 cut(s) 504, 893, 1589, 1706
Eco81I CCTNAGG 1 cut(s) 1342
EcoNI CCTNNNNNAGG 3 cut(s) 328, 813, 2102
EcoO109I RGGNCCY 2 cut(s) 581, 2236
EcoRI GAATTC 3 cut(s) 441, 863, 1617
EcoRII CCWGG 1 cut(s) 1230
EcoRV GATATC 1 cut(s) 3078
EcoT14I CCWWGG 4 cut(s) 597, 1159, 1681, 3065
EcoT22I ATGCAT 1 cut(s) 112
ErhI CCWWGG 4 cut(s) 597, 1159, 1681, 3065
FalI AAGNNNNNCTT 2 cut(s) 2184, 2216
FaqI GGGAC 1 cut(s) 594
FauI CCCGC 2 cut(s) 961, 1171
FauNDI CATATG 1 cut(s) 12
FbaI TGATCA 3 cut(s) 385, 2025, 2203
FblI GTMKAC 3 cut(s) 241, 2187, 3135
Fnu4HI GCNGC 6 cut(s) 492, 1063, 1289, 1919, 2338, 2685
FokI GGATG 7 cut(s) 572, 742, 1650, 2016, 2600, 2741, 3125
Fsp4HI GCNGC 6 cut(s) 492, 1063, 1289, 1919, 2338, 2685
FspBI CTAG 7 cut(s) 416, 560, 671, 1010, 2343, 2378, 2885
FspI TGCGCA 1 cut(s) 28
GlaI GCGC 2 cut(s) 28, 2008
GluI GCNGC 6 cut(s) 492, 1063, 1289, 1919, 2338, 2685
GsuI CTGGAG 1 cut(s) 2673
HaeIII GGCC 7 cut(s) 342, 675, 819, 884, 2713, 2834, 3154
HapII CCGG 4 cut(s) 507, 881, 1262, 2305
HgaI GACGC 1 cut(s) 287
HhaI GCGC 2 cut(s) 29, 2009
Hin6I GCGC 2 cut(s) 27, 2007
HinP1I GCGC 2 cut(s) 27, 2007
HincII GTYRAC 2 cut(s) 451, 3136
HindII GTYRAC 2 cut(s) 451, 3136
HindIII AAGCTT 5 cut(s) 542, 685, 905, 914, 1460
HpaII CCGG 4 cut(s) 507, 881, 1262, 2305
HphI GGTGA 5 cut(s) 67, 294, 1345, 1841, 2603
Hpy166II GTNNAC 9 cut(s) 208, 242, 451, 1240, 1248, 2188, 2519, 3094, 3136
Hpy8I GTNNAC 9 cut(s) 208, 242, 451, 1240, 1248, 2188, 2519, 3094, 3136
Hpy99I CGWCG 1 cut(s) 3147
HpyCH4III ACNGT 3 cut(s) 658, 846, 2656
HpyCH4IV ACGT 3 cut(s) 2220, 2556, 3006
HpyF10VI GCNNNNNNNGC 8 cut(s) 13, 363, 507, 913, 1531, 2006, 2343, 2719
HpyF3I CTNAG 7 cut(s) 329, 903, 1085, 1342, 1463, 1643, 2294
HpySE526I ACGT 3 cut(s) 2220, 2556, 3006
HspAI GCGC 2 cut(s) 27, 2007
KflI GGGWCCC 1 cut(s) 581
KpnI GGTACC 1 cut(s) 1838
Ksp22I TGATCA 3 cut(s) 385, 2025, 2203
LguI GCTCTTC 1 cut(s) 2016
LmnI GCTCC 4 cut(s) 4, 2258, 2692, 3100
Lsp1109I GCAGC 6 cut(s) 503, 1074, 1275, 1930, 2324, 2671
LweI GCATC 2 cut(s) 256, 1994
MaeI CTAG 7 cut(s) 416, 560, 671, 1010, 2343, 2378, 2885
MaeII ACGT 3 cut(s) 2220, 2556, 3006
MaeIII GTNAC 6 cut(s) 846, 1278, 1418, 2537, 2920, 2936
MfeI CAATTG 2 cut(s) 702, 1358
MflI RGATCY 5 cut(s) 550, 1329, 1732, 2745, 2863
MhlI GDGCHC 1 cut(s) 656
MlyI GAGTC 3 cut(s) 194, 2604, 3036
MmeI TCCRAC 3 cut(s) 411, 862, 3050
Mph1103I ATGCAT 1 cut(s) 112
MroXI GAANNNNTTC 2 cut(s) 1018, 1676
MseI TTAA 7 cut(s) 473, 747, 792, 1217, 2826, 2987, 3033
MslI CAYNNNNRTG 1 cut(s) 3005
MspCI CTTAAG 1 cut(s) 3032
MspI CCGG 4 cut(s) 507, 881, 1262, 2305
MspR9I CCNGG 2 cut(s) 881, 1232
MunI CAATTG 2 cut(s) 702, 1358
Mva1269I GAATGC 3 cut(s) 1401, 1542, 2288
MvaI CCWGG 1 cut(s) 1232
MwoI GCNNNNNNNGC 8 cut(s) 13, 363, 507, 913, 1531, 2006, 2343, 2719
NciI CCSGG 1 cut(s) 881
NcoI CCATGG 1 cut(s) 597
NdeI CATATG 1 cut(s) 12
NmeAIII GCCGAG 1 cut(s) 2574
NmuCI GTSAC 2 cut(s) 2537, 2920
NsbI TGCGCA 1 cut(s) 28
NsiI ATGCAT 1 cut(s) 112
NspI RCATGY 4 cut(s) 829, 1932, 2337, 3051
NspV TTCGAA 1 cut(s) 1855
OliI CACNNNNGTG 1 cut(s) 3005
PaeI GCATGC 2 cut(s) 1932, 3051
PagI TCATGA 2 cut(s) 54, 2506
PceI AGGCCT 1 cut(s) 819
PciSI GCTCTTC 1 cut(s) 2016
PctI GAATGC 3 cut(s) 1401, 1542, 2288
PdmI GAANNNNTTC 2 cut(s) 1018, 1676
PfeI GAWTC 9 cut(s) 370, 477, 604, 1168, 1186, 1672, 2142, 2197, 3059
PflFI GACNNNGTC 1 cut(s) 2576
PflMI CCANNNNNTGG 2 cut(s) 311, 1415
PkrI GCNGC 6 cut(s) 493, 1064, 1290, 1920, 2339, 2686
Ple19I CGATCG 1 cut(s) 130
PleI GAGTC 3 cut(s) 194, 2604, 3036
PpsI GAGTC 3 cut(s) 194, 2604, 3036
Ppu21I YACGTR 1 cut(s) 3007
PpuMI RGGWCCY 2 cut(s) 581, 2236
PshAI GACNNNNGTC 1 cut(s) 3141
PsiI TTATAA 1 cut(s) 1043
Psp1406I AACGTT 2 cut(s) 2220, 2556
Psp5II RGGWCCY 2 cut(s) 581, 2236
Psp6I CCWGG 1 cut(s) 1230
PspGI CCWGG 1 cut(s) 1230
PspPI GGNCC 3 cut(s) 581, 2236, 2711
PspPPI RGGWCCY 2 cut(s) 581, 2236
PstI CTGCAG 2 cut(s) 1437, 2080
PstNI CAGNNNCTG 1 cut(s) 281
PsuI RGATCY 5 cut(s) 550, 1329, 1732, 2745, 2863
PsyI GACNNNGTC 1 cut(s) 2576
PvuI CGATCG 1 cut(s) 130
RsaI GTAC 4 cut(s) 1836, 1944, 2630, 2696
RsaNI GTAC 4 cut(s) 1835, 1943, 2629, 2695
RseI CAYNNNNRTG 1 cut(s) 3005
SalI GTCGAC 1 cut(s) 3134
SapI GCTCTTC 1 cut(s) 2016
SaqAI TTAA 7 cut(s) 473, 747, 792, 1217, 2826, 2987, 3033
SatI GCNGC 6 cut(s) 492, 1063, 1289, 1919, 2338, 2685
Sau96I GGNCC 3 cut(s) 581, 2236, 2711
SchI GAGTC 3 cut(s) 194, 2604, 3036
ScrFI CCNGG 2 cut(s) 881, 1232
SduI GDGCHC 1 cut(s) 656
SfaNI GCATC 2 cut(s) 256, 1994
SfcI CTRYAG 6 cut(s) 997, 1122, 1433, 1775, 2076, 2734
SfuI TTCGAA 1 cut(s) 1855
SinI GGWCC 2 cut(s) 581, 2236
SmiMI CAYNNNNRTG 1 cut(s) 3005
SmlI CTYRAG 1 cut(s) 3032
SmoI CTYRAG 1 cut(s) 3032
SphI GCATGC 2 cut(s) 1932, 3051
SseBI AGGCCT 1 cut(s) 819
SsiI CCGC 4 cut(s) 499, 806, 954, 1178
SspI AATATT 3 cut(s) 1296, 1414, 2470
SspMI CTAG 7 cut(s) 416, 560, 671, 1010, 2343, 2378, 2885
StuI AGGCCT 1 cut(s) 819
StyD4I CCNGG 2 cut(s) 879, 1230
StyI CCWWGG 4 cut(s) 597, 1159, 1681, 3065
TaaI ACNGT 3 cut(s) 658, 846, 2656
TaiI ACGT 3 cut(s) 2223, 2559, 3009
TaqI TCGA 6 cut(s) 34, 467, 1855, 2952, 3135, 3145
TatI WGTACW 1 cut(s) 2628
TfiI GAWTC 9 cut(s) 370, 477, 604, 1168, 1186, 1672, 2142, 2197, 3059
Tru1I TTAA 7 cut(s) 473, 747, 792, 1217, 2826, 2987, 3033
Tru9I TTAA 7 cut(s) 473, 747, 792, 1217, 2826, 2987, 3033
TscAI CASTG 4 cut(s) 1590, 2028, 2085, 2788
TseFI GTSAC 2 cut(s) 2537, 2920
TseI GCWGC 6 cut(s) 491, 1062, 1288, 1918, 2337, 2684
Tsp45I GTSAC 2 cut(s) 2537, 2920
TspGWI ACGGA 4 cut(s) 193, 794, 2154, 3131
TspRI CASTG 4 cut(s) 1590, 2028, 2085, 2788
Tth111I GACNNNGTC 1 cut(s) 2576
Van91I CCANNNNNTGG 2 cut(s) 311, 1415
Vha464I CTTAAG 1 cut(s) 3032
VpaK11BI GGWCC 2 cut(s) 581, 2236
XagI CCTNNNNNAGG 3 cut(s) 328, 813, 2102
XapI RAATTY 7 cut(s) 146, 441, 513, 863, 967, 1617, 2983
XbaI TCTAGA 2 cut(s) 559, 2884
XceI RCATGY 4 cut(s) 829, 1932, 2337, 3051
XmiI GTMKAC 3 cut(s) 241, 2187, 3135
XmnI GAANNNNTTC 2 cut(s) 1018, 1676
XspI CTAG 7 cut(s) 416, 560, 671, 1010, 2343, 2378, 2885
Zsp2I ATGCAT 1 cut(s) 112
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.