Belongs to the protein kinase superfamily. Ser Thr protein kinase family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 1114 |
| Acc36I | ACCTGC | 1 cut(s) | 1114 |
| Acc65I | GGTACC | 1 cut(s) | 43 |
| AccB1I | GGYRCC | 1 cut(s) | 43 |
| AccI | GTMKAC | 1 cut(s) | 1286 |
| AciI | CCGC | 1 cut(s) | 378 |
| AclI | AACGTT | 1 cut(s) | 762 |
| AclWI | GGATC | 3 cut(s) | 379, 1064, 1077 |
| AcsI | RAATTY | 2 cut(s) | 299, 1189 |
| AfaI | GTAC | 3 cut(s) | 45, 150, 553 |
| AfeI | AGCGCT | 1 cut(s) | 1308 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 310 |
| AflII | CTTAAG | 1 cut(s) | 1238 |
| AflIII | ACRYGT | 1 cut(s) | 539 |
| AgsI | TTSAA | 9 cut(s) | 13, 25, 85, 178, 455, 577, 655, 751, 1015 |
| AjnI | CCWGG | 1 cut(s) | 1045 |
| AleI | CACNNNNGTG | 1 cut(s) | 1211 |
| AluBI | AGCT | 9 cut(s) | 127, 239, 338, 467, 581, 788, 863, 1325, 1371 |
| AluI | AGCT | 9 cut(s) | 127, 239, 338, 467, 581, 788, 863, 1325, 1371 |
| AlwI | GGATC | 3 cut(s) | 379, 1064, 1077 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 1106 |
| Aor51HI | AGCGCT | 1 cut(s) | 1308 |
| AoxI | GGCC | 1 cut(s) | 1358 |
| ApeKI | GCWGC | 3 cut(s) | 124, 1100, 1325 |
| ApoI | RAATTY | 2 cut(s) | 299, 1189 |
| Asp718I | GGTACC | 1 cut(s) | 43 |
| AspLEI | GCGC | 1 cut(s) | 1309 |
| AspS9I | GGNCC | 1 cut(s) | 1297 |
| AsuHPI | GGTGA | 2 cut(s) | 50, 809 |
| AsuII | TTCGAA | 1 cut(s) | 61 |
| AvaII | GGWCC | 1 cut(s) | 1297 |
| BamHI | GGATCC | 1 cut(s) | 1069 |
| BanI | GGYRCC | 1 cut(s) | 43 |
| BauI | CACGAG | 2 cut(s) | 789, 956 |
| BbsI | GAAGAC | 1 cut(s) | 1057 |
| BbvI | GCAGC | 3 cut(s) | 136, 1112, 1312 |
| BccI | CCATC | 1 cut(s) | 928 |
| BciT130I | CCWGG | 1 cut(s) | 1047 |
| BciVI | GTATCC | 1 cut(s) | 1218 |
| BfaI | CTAG | 1 cut(s) | 584 |
| BfmI | CTRYAG | 1 cut(s) | 282 |
| BfoI | RGCGCY | 1 cut(s) | 1310 |
| BfrI | CTTAAG | 1 cut(s) | 1238 |
| BfuAI | ACCTGC | 1 cut(s) | 1114 |
| BfuI | GTATCC | 1 cut(s) | 1218 |
| BglII | AGATCT | 1 cut(s) | 531 |
| BisI | GCNGC | 3 cut(s) | 125, 1101, 1326 |
| BlsI | GCNGC | 3 cut(s) | 126, 1102, 1327 |
| Bme1390I | CCNGG | 1 cut(s) | 1047 |
| Bme18I | GGWCC | 1 cut(s) | 1297 |
| BmgT120I | GGNCC | 1 cut(s) | 1297 |
| BmiI | GGNNCC | 2 cut(s) | 45, 1071 |
| BmrFI | CCNGG | 1 cut(s) | 1047 |
| BmsI | GCATC | 2 cut(s) | 200, 942 |
| BoxI | GACNNNNGTC | 1 cut(s) | 1347 |
| BpiI | GAAGAC | 1 cut(s) | 1057 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 244, 276 |
| BpmI | CTGGAG | 1 cut(s) | 53 |
| Bpu14I | TTCGAA | 1 cut(s) | 61 |
| BsaJI | CCNNGG | 2 cut(s) | 198, 1271 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 310 |
| Bse1I | ACTGG | 2 cut(s) | 549, 1339 |
| Bse3DI | GCAATG | 2 cut(s) | 499, 504 |
| BseBI | CCWGG | 1 cut(s) | 1047 |
| BseDI | CCNNGG | 2 cut(s) | 198, 1271 |
| BseGI | GGATG | 4 cut(s) | 215, 940, 957, 1287 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 310 |
| BseMI | GCAATG | 2 cut(s) | 499, 504 |
| BseNI | ACTGG | 2 cut(s) | 549, 1339 |
| BseRI | GAGGAG | 4 cut(s) | 265, 632, 829, 1307 |
| BseXI | GCAGC | 3 cut(s) | 136, 1112, 1312 |
| BseYI | CCCAGC | 1 cut(s) | 863 |
| BshFI | GGCC | 1 cut(s) | 1360 |
| BshNI | GGYRCC | 1 cut(s) | 43 |
| BsiSI | CCGG | 1 cut(s) | 511 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 310 |
| BsmI | GAATGC | 1 cut(s) | 494 |
| BsnI | GGCC | 1 cut(s) | 1360 |
| Bsp119I | TTCGAA | 1 cut(s) | 61 |
| Bsp143I | GATC | 4 cut(s) | 371, 531, 732, 1069 |
| BspACI | CCGC | 1 cut(s) | 378 |
| BspANI | GGCC | 1 cut(s) | 1360 |
| BspHI | TCATGA | 1 cut(s) | 712 |
| BspLI | GGNNCC | 2 cut(s) | 45, 1071 |
| BspMAI | CTGCAG | 1 cut(s) | 286 |
| BspMI | ACCTGC | 1 cut(s) | 1114 |
| BspPI | GGATC | 3 cut(s) | 379, 1064, 1077 |
| BspT104I | TTCGAA | 1 cut(s) | 61 |
| BspT107I | GGYRCC | 1 cut(s) | 43 |
| BspTI | CTTAAG | 1 cut(s) | 1238 |
| BsrDI | GCAATG | 2 cut(s) | 499, 504 |
| BsrI | ACTGG | 2 cut(s) | 549, 1339 |
| BssECI | CCNNGG | 2 cut(s) | 198, 1271 |
| BssMI | GATC | 4 cut(s) | 371, 531, 732, 1069 |
| BssSI | CACGAG | 2 cut(s) | 789, 956 |
| BssT1I | CCWWGG | 1 cut(s) | 198 |
| Bst2BI | CACGAG | 2 cut(s) | 789, 956 |
| Bst2UI | CCWGG | 1 cut(s) | 1047 |
| Bst6I | CTCTTC | 1 cut(s) | 1083 |
| BstAFI | CTTAAG | 1 cut(s) | 1238 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1106 |
| BstBI | TTCGAA | 1 cut(s) | 61 |
| BstC8I | GCNNGC | 2 cut(s) | 136, 1255 |
| BstDEI | CTNAG | 2 cut(s) | 235, 356 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 308 |
| BstF5I | GGATG | 4 cut(s) | 215, 940, 957, 1287 |
| BstH2I | RGCGCY | 1 cut(s) | 1310 |
| BstHHI | GCGC | 1 cut(s) | 1309 |
| BstKTI | GATC | 4 cut(s) | 374, 534, 735, 1072 |
| BstMBI | GATC | 4 cut(s) | 371, 531, 732, 1069 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 384, 961, 1106 |
| BstNI | CCWGG | 1 cut(s) | 1047 |
| BstNSI | RCATGY | 3 cut(s) | 138, 543, 1257 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 1347 |
| BstSCI | CCNGG | 1 cut(s) | 1045 |
| BstSFI | CTRYAG | 1 cut(s) | 282 |
| BstV1I | GCAGC | 3 cut(s) | 136, 1112, 1312 |
| BstV2I | GAAGAC | 1 cut(s) | 1057 |
| BstX2I | RGATCY | 2 cut(s) | 531, 1069 |
| BstYI | RGATCY | 2 cut(s) | 531, 1069 |
| BsuI | GTATCC | 1 cut(s) | 1218 |
| BsuRI | GGCC | 1 cut(s) | 1360 |
| BtsCI | GGATG | 4 cut(s) | 215, 940, 957, 1287 |
| BtsI | GCAGTG | 1 cut(s) | 291 |
| BtsIMutI | CAGTG | 3 cut(s) | 291, 1144, 1332 |
| BveI | ACCTGC | 1 cut(s) | 1114 |
| Cac8I | GCNNGC | 2 cut(s) | 136, 1255 |
| CaiI | CAGNNNCTG | 1 cut(s) | 1106 |
| CciI | TCATGA | 1 cut(s) | 712 |
| CfoI | GCGC | 1 cut(s) | 1309 |
| Cfr13I | GGNCC | 1 cut(s) | 1297 |
| Csp6I | GTAC | 3 cut(s) | 44, 149, 552 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 85, 120 |
| CviAII | CATG | 5 cut(s) | 135, 540, 713, 781, 1254 |
| CviQI | GTAC | 3 cut(s) | 44, 149, 552 |
| DdeI | CTNAG | 2 cut(s) | 235, 356 |
| DpnI | GATC | 4 cut(s) | 373, 533, 734, 1071 |
| DpnII | GATC | 4 cut(s) | 371, 531, 732, 1069 |
| DraI | TTTAAA | 2 cut(s) | 183, 1003 |
| Eam1104I | CTCTTC | 1 cut(s) | 1083 |
| EarI | CTCTTC | 1 cut(s) | 1083 |
| Eco130I | CCWWGG | 1 cut(s) | 198 |
| Eco47I | GGWCC | 1 cut(s) | 1297 |
| Eco47III | AGCGCT | 1 cut(s) | 1308 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 308 |
| EcoRII | CCWGG | 1 cut(s) | 1045 |
| EcoT14I | CCWWGG | 1 cut(s) | 198 |
| ErhI | CCWWGG | 1 cut(s) | 198 |
| FaeI | CATG | 5 cut(s) | 138, 543, 716, 784, 1257 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 390, 422 |
| FatI | CATG | 5 cut(s) | 134, 539, 712, 780, 1253 |
| FblI | GTMKAC | 1 cut(s) | 1286 |
| Fnu4HI | GCNGC | 3 cut(s) | 125, 1101, 1326 |
| FokI | GGATG | 4 cut(s) | 222, 947, 964, 1294 |
| Fsp4HI | GCNGC | 3 cut(s) | 125, 1101, 1326 |
| FspBI | CTAG | 1 cut(s) | 584 |
| GlaI | GCGC | 1 cut(s) | 1308 |
| GluI | GCNGC | 3 cut(s) | 125, 1101, 1326 |
| GsaI | CCCAGC | 1 cut(s) | 867 |
| GsuI | CTGGAG | 1 cut(s) | 53 |
| HaeII | RGCGCY | 1 cut(s) | 1310 |
| HaeIII | GGCC | 1 cut(s) | 1360 |
| HapII | CCGG | 1 cut(s) | 511 |
| HhaI | GCGC | 1 cut(s) | 1309 |
| Hin1II | CATG | 5 cut(s) | 138, 543, 716, 784, 1257 |
| Hin6I | GCGC | 1 cut(s) | 1307 |
| HinP1I | GCGC | 1 cut(s) | 1307 |
| HincII | GTYRAC | 1 cut(s) | 1342 |
| HindII | GTYRAC | 1 cut(s) | 1342 |
| HinfI | GANTC | 6 cut(s) | 4, 348, 403, 816, 836, 1265 |
| HpaII | CCGG | 1 cut(s) | 511 |
| HphI | GGTGA | 2 cut(s) | 50, 809 |
| Hpy166II | GTNNAC | 7 cut(s) | 189, 725, 972, 1129, 1287, 1300, 1342 |
| Hpy188I | TCNGA | 3 cut(s) | 118, 1261, 1272 |
| Hpy188III | TCNNGA | 5 cut(s) | 32, 413, 713, 924, 1352 |
| Hpy8I | GTNNAC | 7 cut(s) | 189, 725, 972, 1129, 1287, 1300, 1342 |
| HpyAV | CCTTC | 3 cut(s) | 314, 461, 668 |
| HpyCH4IV | ACGT | 1 cut(s) | 762 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 384, 961, 1106 |
| HpyF3I | CTNAG | 2 cut(s) | 235, 356 |
| HpySE526I | ACGT | 1 cut(s) | 762 |
| Hsp92II | CATG | 5 cut(s) | 138, 543, 716, 784, 1257 |
| HspAI | GCGC | 1 cut(s) | 1307 |
| KpnI | GGTACC | 1 cut(s) | 47 |
| Kzo9I | GATC | 4 cut(s) | 371, 531, 732, 1069 |
| LmnI | GCTCC | 2 cut(s) | 464, 898 |
| Lsp1109I | GCAGC | 3 cut(s) | 136, 1112, 1312 |
| LweI | GCATC | 2 cut(s) | 200, 942 |
| MaeI | CTAG | 1 cut(s) | 584 |
| MaeII | ACGT | 1 cut(s) | 762 |
| MaeIII | GTNAC | 1 cut(s) | 1142 |
| MalI | GATC | 4 cut(s) | 373, 533, 734, 1071 |
| MboI | GATC | 4 cut(s) | 371, 531, 732, 1069 |
| MboII | GAAGA | 8 cut(s) | 428, 541, 701, 758, 1062, 1070, 1181, 1274 |
| MfeI | CAATTG | 1 cut(s) | 879 |
| MflI | RGATCY | 2 cut(s) | 531, 1069 |
| MluCI | AATT | 7 cut(s) | 119, 178, 192, 299, 366, 879, 1189 |
| MlyI | GAGTC | 2 cut(s) | 810, 830 |
| MslI | CAYNNNNRTG | 1 cut(s) | 1211 |
| MspA1I | CMGCKG | 1 cut(s) | 863 |
| MspCI | CTTAAG | 1 cut(s) | 1238 |
| MspI | CCGG | 1 cut(s) | 511 |
| MspR9I | CCNGG | 1 cut(s) | 1047 |
| MunI | CAATTG | 1 cut(s) | 879 |
| Mva1269I | GAATGC | 1 cut(s) | 494 |
| MvaI | CCWGG | 1 cut(s) | 1047 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 384, 961, 1106 |
| NdeII | GATC | 4 cut(s) | 371, 531, 732, 1069 |
| NlaIII | CATG | 5 cut(s) | 138, 543, 716, 784, 1257 |
| NlaIV | GGNNCC | 2 cut(s) | 45, 1071 |
| NmeAIII | GCCGAG | 1 cut(s) | 780 |
| NspI | RCATGY | 3 cut(s) | 138, 543, 1257 |
| NspV | TTCGAA | 1 cut(s) | 61 |
| OliI | CACNNNNGTG | 1 cut(s) | 1211 |
| PaeI | GCATGC | 2 cut(s) | 138, 1257 |
| PagI | TCATGA | 1 cut(s) | 712 |
| PaqCI | CACCTGC | 1 cut(s) | 1114 |
| PciI | ACATGT | 1 cut(s) | 539 |
| PctI | GAATGC | 1 cut(s) | 494 |
| PfeI | GAWTC | 4 cut(s) | 4, 348, 403, 1265 |
| PkrI | GCNGC | 3 cut(s) | 126, 1102, 1327 |
| PleI | GAGTC | 2 cut(s) | 810, 830 |
| PpsI | GAGTC | 2 cut(s) | 810, 830 |
| PscI | ACATGT | 1 cut(s) | 539 |
| PshAI | GACNNNNGTC | 1 cut(s) | 1347 |
| Psp1406I | AACGTT | 1 cut(s) | 762 |
| Psp6I | CCWGG | 1 cut(s) | 1045 |
| PspFI | CCCAGC | 1 cut(s) | 863 |
| PspGI | CCWGG | 1 cut(s) | 1045 |
| PspN4I | GGNNCC | 2 cut(s) | 45, 1071 |
| PspPI | GGNCC | 1 cut(s) | 1297 |
| PstI | CTGCAG | 1 cut(s) | 286 |
| PstNI | CAGNNNCTG | 1 cut(s) | 1106 |
| PsuI | RGATCY | 2 cut(s) | 531, 1069 |
| PvuII | CAGCTG | 1 cut(s) | 863 |
| RsaI | GTAC | 3 cut(s) | 45, 150, 553 |
| RsaNI | GTAC | 3 cut(s) | 44, 149, 552 |
| RseI | CAYNNNNRTG | 1 cut(s) | 1211 |
| SatI | GCNGC | 3 cut(s) | 125, 1101, 1326 |
| Sau3AI | GATC | 4 cut(s) | 371, 531, 732, 1069 |
| Sau96I | GGNCC | 1 cut(s) | 1297 |
| SchI | GAGTC | 2 cut(s) | 810, 830 |
| ScrFI | CCNGG | 1 cut(s) | 1047 |
| SfaNI | GCATC | 2 cut(s) | 200, 942 |
| SfcI | CTRYAG | 1 cut(s) | 282 |
| SfuI | TTCGAA | 1 cut(s) | 61 |
| SinI | GGWCC | 1 cut(s) | 1297 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 1211 |
| SmlI | CTYRAG | 1 cut(s) | 1238 |
| SmoI | CTYRAG | 1 cut(s) | 1238 |
| SphI | GCATGC | 2 cut(s) | 138, 1257 |
| Sse9I | AATT | 7 cut(s) | 119, 178, 192, 299, 366, 879, 1189 |
| SsiI | CCGC | 1 cut(s) | 378 |
| SspI | AATATT | 1 cut(s) | 676 |
| SspMI | CTAG | 1 cut(s) | 584 |
| StyD4I | CCNGG | 1 cut(s) | 1045 |
| StyI | CCWWGG | 1 cut(s) | 198 |
| TaiI | ACGT | 1 cut(s) | 765 |
| TaqI | TCGA | 4 cut(s) | 61, 334, 1158, 1351 |
| TasI | AATT | 7 cut(s) | 119, 178, 192, 299, 366, 879, 1189 |
| TatI | WGTACW | 1 cut(s) | 551 |
| TfiI | GAWTC | 4 cut(s) | 4, 348, 403, 1265 |
| TscAI | CASTG | 3 cut(s) | 291, 1144, 1339 |
| TseI | GCWGC | 3 cut(s) | 124, 1100, 1325 |
| TspDTI | ATGAA | 8 cut(s) | 306, 320, 650, 701, 1137, 1167, 1182, 1217 |
| TspGWI | ACGGA | 2 cut(s) | 360, 1304 |
| TspRI | CASTG | 3 cut(s) | 291, 1144, 1339 |
| Vha464I | CTTAAG | 1 cut(s) | 1238 |
| VpaK11BI | GGWCC | 1 cut(s) | 1297 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 308 |
| XapI | RAATTY | 2 cut(s) | 299, 1189 |
| XceI | RCATGY | 3 cut(s) | 138, 543, 1257 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 478 |
| XmiI | GTMKAC | 1 cut(s) | 1286 |
| XspI | CTAG | 1 cut(s) | 584 |