Rw1G032180
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0000248
Physical Location & Seq
Forward (+)
60475693 .. 60486415
10723 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G032180.1

Sequence Viewer

Length: 3159 bp
ATGGAGTTTATTCATGTACCCAGCTTCTGTGCCTTTTGCTCTATATGCCTTCGTGTGATTTCTTTCCTCCTTTACATAAACTTTTTCCAATCTACCATTTTTGTTGCGAATGCGCTTGGTGGAAATGAAACTGATCAATTCGCTTTGCTCAAATTCAAGGAAGCCATAGTCAATGACCCAAAAGGGTTGTTGAACTCGTGGAATGATTCCATTCACTTCTGCAATTGGCATGGAGTTGCTTGCAGCAGAAGGCATCAAAGAGTTGTAGCCTTGAACCTTTCAGATAGTGATTTGCATGGAAGCATATCACCTTATATTGGCAACCTCTCGTTTCTAAGGTTCATCAACCTTGATTATAACAGATTCTATGGCAAGATCCCACAGCAAGTTAATCATTTGTTCCGACTGCGCCATCTCAATCTCACTCAGAACATGTTGGAGGGGGGAATTCTGGTCAACCTGACCTTCTGTCCTGATCTGAGCATCATAAGTTTTGGAATAAACCGCCTTACAGGTGAAATTCCTTCAGAGATGGGCTCATTCACGAAGCTCATTTATCTGAATCTAGAGACAAACAATCTGACAGGAGGCATCCCACCTTCCTTGGGAAATCTTTCATCACTCACAATGTTGTCCTTAGTATATAACAATTTGGTGGGCAATGTTCCAGAGGAGTTAGGACGACTGCAAAGCTTATCTACATTTTATATTGGTGCCAATAAACTCTCTGGTATGATCCCTGCCCCCATTTTTAACATATCATCTATGACCCTCTTCTCAATCACAGCTAATGCGTTTAAGGGCACTTTACCACCCTCCATGGGTCTAAACATGCCTAATCTACAAAAAGTTTCCCTTGGTGGAAATGAATTCTCTGGGCAAATTCCTAGTTCTTTGTCTTCCAATGCTTCTCAGCTTCAGTTGTTTGATATTTCGAAGAATAATTTTGTGGGGCAAGTTCCTGCAAACTTTGGAAATCTTCCCAAGCTGCAGTTGCTAGTCATAGGGTCGAATAATCTTGGAACTAATTCATCTAATGATTTGGGATTTATAGCATCCTTGAAAAATTGCAGCAATCTGAATATTCTTGATATAGGTTATAACAGTTTTGGAGGTGTTTTACCAAACTCTATAGCCAACTTGTCAACCCAACTGACTCACCTCTACCTTGGAGGGATTCTTGTAACAGGAATCATTCCTGCAACATTAGAAAATCTCCACAATTTGATAATCTTGGGCTTGGAGAATAACCTATTCAGAGGAGTCATTCCTGCTTCTTTTGGGAAATTACAAATGCTGCAATCGTTGCTATTACATGGTAACAGATTATCAGGACAGATCCCATCTTCCATAGGAAACCTCACACAATTATTTGAGCTCTACCTATTCAAGAATAGACTAGAGGGAAACATTCCTCCAAGTCTCCAAAACTGCCAACATTTGCAGTATATGAGAATATCAGAAAATAGGCTTAGTGGAGATATACCACCACAGGTTATTGGTCTTTCCTCACTTCTCTATCTCAGGTTATCACAGAACTCGTTTACTGGCAGTTTGCCTGTGGAAGTGGGTAAGCTGAAGAATATCTATAAACTGGACGTCTCTGATAATAATTTGACTGGAGAAATTCCAGAAATTATTAGAAGCTGTCTGAGCCTTGAATTTCTTGTACTACAAGGGAATCTCTTTCAAGGAATCATACCTTCTTCTTTGGCTTCTTTGAGAGGTCTTCAGTATCTAGATCTTTCACGAAACAACTTGTCAGGATATATTCCAAAAGACCTACAGAGACTTACATTCTTGATATATTTGAACCTTTCGTTCAATAGTCTGGAGGGTGAGGTGCCAACAAAAGGAGTATTTCGAAACACAACCGCAATAACATTGATTGGAAATACAAAACTTTGCGGTGGTATTTCAGAATTGCAGCTACCAGCATGCCTCATCAAAGTCCCAAAGCAGAGAAAGTTGCATGGTTTCAAACTAAAGTTCACAATTTCTTTAGTCGTCGGATGCTCTCTTCTATTTGCAATCATCTTAGCTCTTTATTGGAGGAGAAAAACTCAAAAGAAGAAACCATTATCTGCAGTGTCATCAATCAACTTCCTTTCAAAAGTTTCGTACCAAACACTTCATCAAGCTACTGCCGGATTCTCTCCGAGCAATCAAATTGGATCAGGCGGTTTTGGCTCTGTATACAAAGGGATTCTTGCTCAAGAAGAAAACAAAGTTGTTGCCATAAAGGTCCTCAACCTTCAACATAAAGAAGCTTCTAAGAGTTTTGTGGCTGAATGCAATGCACTGAGAAATATCCGGCACAGGAATCTTGTGAAGATCTTAACATGTTGCTCCAGCACGGATTACAATGGTAATGACTTTAAAGCTCTAGTATTTGAATACATGTCAAACGGAAGCTTAGAGGAGTGGCTGCACAGTGAAGACCAATCAAGGAGCTTGAACCTTCTTCAAAGATTGAGTATTTCTATCGATGTGGCTTCTGCATTATGTTATCTTCATGACTATTGTGAACCACAAATCATTCATCGTGACATAAAGCCAAGCAACGTTCTTCTTGACGATGACAAGGTTGCTCGTGTCAGTGATTTTGGGCTAGCAAGACTCATCCCAACTACCATCGACTCCTCTGAAAATCAAAGTAGCACAATTGGAATAAAGGGAACAATCGGCTATGCTGCTCCAGAGTATGCAAGTGGTGTTGAAGCATCAAGACAAGGAGATGTATATAGTTATGGGATCCTTGTATTGGAAATGTTCACGGGAAGAAGACCCACCGATGAAATGTTCAAAGACGGTTTGAATCTCCACAACTTCGTTAAGACGGCAATACTAGGAAGAATTGTGCAGATTGTGGACCCTGCTCTTCTCGCCACTGGAGAAGTGACAGCTCCTGCAGCAGCAAAAAACAAAGTGAACTACATGCTTAGAGGTCATAACAATGAAATTGAAGCAGAAGAGGAAAATAGAGATTATGAGAACCTAAGCAAGATGGACACACATGTGTGGAAGTGTATACATTCAATCCTTGAGATAGGACTGGCGTGCTCGGAGGAATCACCAAAGAATAGAATGTGTATGGAGGATGTCGTCAGGGACCTCCACCATGTACAAAATGCTTTCAATGGTGCTGAGATCAGTTGA

Protein Analysis

1052

Amino Acids

115.99

Weight (kDa)

8.11

Isoelectric Point (pI)

41.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 43 - 82 9.4e-11 Leucine rich repeat N-terminal domain
LRR_14 PF23598 100 - 338 6e-11 Leucine-rich repeat region
LRR_8 PF13855 384 - 443 2.8e-07 Leucine rich repeat
LRR_14 PF23598 402 - 486 2.7e-07 Leucine-rich repeat region
LRR_14 PF23598 470 - 611 5.1e-10 Leucine-rich repeat region
LRR_8 PF13855 553 - 611 3.3e-08 Leucine rich repeat
Pkinase PF00069 721 - 941 6e-43 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 722 - 935 6.8e-45 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000248)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g31390 FvH4_5g31630 FvH4_5g31631 FvH4_7g24151 FvH4_7g24160 FvH4_7g24240 FvH4_7g24242 FvH4_7g24243 FvH4_7g24510 FvH4_7g24920 FvH4_7g24920 FvH4_7g24920 FvH4_7g29910
malus_domestica MD01G1131800.v1.1 MD01G1150000.v1.1 MD01G1151300.v1.1
prunus_persica Prupe.2G248300_v2.0.a1
pyrus_communis pycom01g16800
rosa_chinensis RchiOBHm_Chr1g0339821 RchiOBHm_Chr1g0345151 RchiOBHm_Chr1g0346851 RchiOBHm_Chr1g0351981 RchiOBHm_Chr1g0369731 RchiOBHm_Chr1g0369841 RchiOBHm_Chr1g0369861 RchiOBHm_Chr1g0369931 RchiOBHm_Chr1g0369961 RchiOBHm_Chr1g0370261 RchiOBHm_Chr1g0370281 RchiOBHm_Chr5g0076391 RchiOBHm_Chr6g0248051 RchiOBHm_Chr6g0248071
rosa_laevigata RLG00000002548 RLG00000013479 RLG00000027042 RLG00000027045 RLG00000027047 RLG00000027060 RLG00000027063 RLG00000027082 RLG00000027084 RLG00000027087 RLG00000027089 RLG00000027090 RLG00000027091 RLG00000027092 RLG00000027094 RLG00000027097 RLG00000027100 RLG00000027101 RLG00000027111 RLG00000028769 RLG00000028851 RLG00000029244 RLG00000030021
rosa_multiflora Rmu_co7968444.1_g000001 Rmu_co8060564.1_g000001 Rmu_co8098766.1_g000001 Rmu_co8172886.1_g000001 Rmu_co8291893.1_g000001 Rmu_co8327887.1_g000001 Rmu_co8406981.1_g000001 Rmu_co8449189.1_g000001 Rmu_co8470871.1_g000001 Rmu_sc0000756.1_g000006 Rmu_sc0001154.1_g000028 Rmu_sc0001565.1_g000055 Rmu_sc0001657.1_g000002 Rmu_sc0002095.1_g000014 Rmu_sc0002147.1_g000001 Rmu_sc0002705.1_g000004 Rmu_sc0002705.1_g000005 Rmu_sc0004087.1_g000025 Rmu_sc0005500.1_g000022 Rmu_sc0006707.1_g000005 Rmu_sc0006707.1_g000009 Rmu_sc0008140.1_g000012 Rmu_sc0008140.1_g000019 Rmu_sc0016176.1_g000001 Rmu_sc0016906.1_g000006 Rmu_sc0028980.1_g000001 Rmu_sc0041369.1_g000001
rosa_roxburghii Rroxscaffold_3G00232610 Rroxscaffold_4G00286690 Rroxscaffold_4G00286920 Rroxscaffold_4G00286930 Rroxscaffold_4G00286960 Rroxscaffold_4G00286980 Rroxscaffold_4G00287030 Rroxscaffold_4G00287040 Rroxscaffold_4G00287070 Rroxscaffold_4G00287090 Rroxscaffold_4G00287330 Rroxscaffold_4G00287430 Rroxscaffold_4G00303380 Rroxscaffold_4G00303400 Rroxscaffold_4G00303410 Rroxscaffold_4G00308100 Rroxscaffold_4G00308480 Rroxscaffold_4G00313700 Rroxscaffold_4G00322910 Rroxscaffold_7G00190680 Rroxscaffold_7G00193490
rosa_rugosa Rorug01G0032200 Rorug01G0139400.1 Rorug01G0139600.1 Rorug01G0139700.1 Rorug01G0184400 Rorug01G0351900 Rorug01G0351900 Rorug01G0352600 Rorug01G0352600 Rorug01G0352800 Rorug01G0355300 Rorug01G0355400 Rorug01G0356700.1 Rorug01G0356800 Rorug06G0090600 Rorug07G0276100
rosa_samantha Rh1AG201900 Rh1AG361300 Rh1AG365100 Rh6AG204000
rosa_wichuraiana Rw0G015480 Rw1G007000 Rw1G008190 Rw1G012880 Rw1G012990 Rw1G016950 Rw1G031720 Rw1G031800 Rw1G031820 Rw1G032020 Rw1G032180 Rw6G017720 Rw7G035600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 357, 1101
AatII GACGTC 1 cut(s) 1602
AccB1I GGYRCC 2 cut(s) 713, 1843
AccI GTMKAC 2 cut(s) 2196, 3031
AciI CCGC 4 cut(s) 505, 1875, 1908, 2181
AclI AACGTT 1 cut(s) 2565
AclWI GGATC 6 cut(s) 370, 730, 1333, 2182, 2749, 2762
AcsI RAATTY 7 cut(s) 152, 447, 519, 869, 882, 1626, 1661
AcuI CTGAAG 4 cut(s) 510, 902, 1598, 1715
AcyI GRCGYC 1 cut(s) 1599
AfaI GTAC 4 cut(s) 18, 1671, 2123, 3126
AfiI CCNNNNNNNGG 5 cut(s) 317, 605, 821, 1853, 2764
AflIII ACRYGT 4 cut(s) 432, 2342, 2400, 3016
AhdI GACNNNNNGTC 1 cut(s) 468
AleI CACNNNNGTG 2 cut(s) 3017, 3019
Alw21I GWGCWC 2 cut(s) 1380, 3065
Alw26I GTCTC 4 cut(s) 563, 1427, 1606, 1783
AlwI GGATC 6 cut(s) 370, 730, 1333, 2182, 2749, 2762
AlwNI CAGNNNCTG 2 cut(s) 27, 2909
ApeKI GCWGC 9 cut(s) 243, 988, 1071, 1297, 1927, 2428, 2693, 2912, 2915
ApoI RAATTY 7 cut(s) 152, 447, 519, 869, 882, 1626, 1661
Asp700I GAANNNNTTC 2 cut(s) 613, 1027
AspLEI GCGC 2 cut(s) 115, 411
AspS9I GGNCC 3 cut(s) 2245, 2872, 3112
AsuHPI GGTGA 5 cut(s) 300, 527, 1151, 1850, 3066
AsuII TTCGAA 2 cut(s) 935, 1864
AsuNHI GCTAGC 1 cut(s) 2611
AvaII GGWCC 3 cut(s) 2245, 2872, 3112
BaeGI GKGCMC 1 cut(s) 806
BamHI GGATCC 1 cut(s) 2754
BanI GGYRCC 2 cut(s) 713, 1843
BanII GRGCYC 2 cut(s) 539, 1380
BauI CACGAG 2 cut(s) 196, 2592
BbsI GAAGAC 4 cut(s) 891, 1721, 2445, 2791
Bbv12I GWGCWC 2 cut(s) 1380, 3065
BbvI GCAGC 9 cut(s) 255, 975, 1083, 1284, 1939, 2415, 2680, 2924, 2927
BccI CCATC 5 cut(s) 420, 526, 1351, 2642, 3001
BceAI ACGGC 1 cut(s) 2856
BclI TGATCA 1 cut(s) 133
BcoDI GTCTC 4 cut(s) 563, 1427, 1606, 1783
BfaI CTAG 8 cut(s) 566, 888, 998, 1400, 1739, 2387, 2612, 2849
BfmI CTRYAG 5 cut(s) 989, 1131, 1784, 2085, 2910
BglII AGATCT 2 cut(s) 1741, 2334
BisI GCNGC 9 cut(s) 244, 989, 1072, 1298, 1928, 2429, 2694, 2913, 2916
BlsI GCNGC 9 cut(s) 245, 990, 1073, 1299, 1929, 2430, 2695, 2914, 2917
Bme18I GGWCC 3 cut(s) 2245, 2872, 3112
BmeRI GACNNNNNGTC 1 cut(s) 468
BmgT120I GGNCC 3 cut(s) 2245, 2872, 3112
BmiI GGNNCC 5 cut(s) 715, 1845, 2756, 2874, 3113
BmsI GCATC 6 cut(s) 262, 492, 600, 1064, 2003, 2732
BmtI GCTAGC 1 cut(s) 2615
BpiI GAAGAC 4 cut(s) 891, 1721, 2445, 2791
BplI GAGNNNNNCTC 4 cut(s) 521, 553, 2047, 2079
BpmI CTGGAG 5 cut(s) 1641, 1853, 2335, 2682, 2913
Bpu10I CCTNAGC 1 cut(s) 2999
Bpu14I TTCGAA 2 cut(s) 935, 1864
BpuEI CTTGAG 2 cut(s) 2199, 3065
Bsa29I ATCGAT 1 cut(s) 2487
BsaHI GRCGYC 1 cut(s) 1599
BsaJI CCNNGG 4 cut(s) 603, 819, 856, 1168
Bsc4I CCNNNNNNNGG 5 cut(s) 317, 605, 821, 1853, 2764
Bse1I ACTGG 5 cut(s) 1552, 1599, 1624, 2896, 3060
Bse3DI GCAATG 2 cut(s) 667, 2302
BseCI ATCGAT 1 cut(s) 2487
BseDI CCNNGG 4 cut(s) 603, 819, 856, 1168
BseGI GGATG 5 cut(s) 591, 1055, 2018, 2622, 3106
BseLI CCNNNNNNNGG 5 cut(s) 317, 605, 821, 1853, 2764
BseMI GCAATG 2 cut(s) 667, 2302
BseMII CTCAG 7 cut(s) 440, 470, 926, 1537, 1643, 2294, 3138
BseNI ACTGG 5 cut(s) 1552, 1599, 1624, 2896, 3060
BseRI GAGGAG 5 cut(s) 686, 1275, 2068, 2435, 2632
BseSI GKGCMC 1 cut(s) 806
BseXI GCAGC 9 cut(s) 255, 975, 1083, 1284, 1939, 2415, 2680, 2924, 2927
BseYI CCCAGC 1 cut(s) 20
BsgI GTGCAG 2 cut(s) 2414, 2882
BshNI GGYRCC 2 cut(s) 713, 1843
BshVI ATCGAT 1 cut(s) 2487
BsiHKAI GWGCWC 2 cut(s) 1380, 3065
BsiSI CCGG 2 cut(s) 2148, 2314
BslFI GGGAC 2 cut(s) 1937, 3125
BslI CCNNNNNNNGG 5 cut(s) 317, 605, 821, 1853, 2764
BsmAI GTCTC 4 cut(s) 563, 1427, 1606, 1783
BsmBI CGTCTC 1 cut(s) 1606
BsmFI GGGAC 2 cut(s) 1937, 3125
BsmI GAATGC 2 cut(s) 115, 2297
Bsp119I TTCGAA 2 cut(s) 935, 1864
Bsp1286I GDGCHC 4 cut(s) 539, 806, 1380, 3065
Bsp1407I TGTACA 1 cut(s) 3124
Bsp19I CCATGG 1 cut(s) 819
BspACI CCGC 4 cut(s) 505, 1875, 1908, 2181
BspCNI CTCAG 7 cut(s) 439, 471, 925, 1536, 1644, 2295, 3139
BspDI ATCGAT 1 cut(s) 2487
BspHI TCATGA 1 cut(s) 2515
BspLI GGNNCC 5 cut(s) 715, 1845, 2756, 2874, 3113
BspMAI CTGCAG 3 cut(s) 993, 2089, 2914
BspOI GCTAGC 1 cut(s) 2615
BspPI GGATC 6 cut(s) 370, 730, 1333, 2182, 2749, 2762
BspQI GCTCTTC 1 cut(s) 2886
BspT104I TTCGAA 2 cut(s) 935, 1864
BspT107I GGYRCC 2 cut(s) 713, 1843
BsrDI GCAATG 2 cut(s) 667, 2302
BsrGI TGTACA 1 cut(s) 3124
BsrI ACTGG 5 cut(s) 1552, 1599, 1624, 2896, 3060
BssECI CCNNGG 4 cut(s) 603, 819, 856, 1168
BssNAI GTATAC 2 cut(s) 2197, 3032
BssNI GRCGYC 1 cut(s) 1599
BssSI CACGAG 2 cut(s) 196, 2592
BssT1I CCWWGG 4 cut(s) 603, 819, 856, 1168
Bst1107I GTATAC 2 cut(s) 2197, 3032
Bst2BI CACGAG 2 cut(s) 196, 2592
Bst4CI ACNGT 3 cut(s) 1106, 2435, 2813
Bst6I CTCTTC 4 cut(s) 779, 2025, 2886, 2967
BstACI GRCGYC 1 cut(s) 1599
BstAPI GCANNNNNTGC 1 cut(s) 1306
BstAUI TGTACA 1 cut(s) 3124
BstBI TTCGAA 2 cut(s) 935, 1864
BstC8I GCNNGC 4 cut(s) 241, 1939, 2613, 3061
BstDSI CCRYGG 1 cut(s) 819
BstF5I GGATG 5 cut(s) 591, 1055, 2018, 2622, 3106
BstHHI GCGC 2 cut(s) 115, 411
BstMAI GTCTC 4 cut(s) 563, 1427, 1606, 1783
BstMWI GCNNNNNNNGC 7 cut(s) 45, 994, 1306, 1653, 2187, 2885, 2912
BstNSI RCATGY 7 cut(s) 436, 835, 1941, 2346, 2404, 2941, 3020
BstSFI CTRYAG 5 cut(s) 989, 1131, 1784, 2085, 2910
BstSLI GKGCMC 1 cut(s) 806
BstV1I GCAGC 9 cut(s) 255, 975, 1083, 1284, 1939, 2415, 2680, 2924, 2927
BstV2I GAAGAC 4 cut(s) 891, 1721, 2445, 2791
BstX2I RGATCY 5 cut(s) 375, 1338, 1741, 2334, 2754
BstYI RGATCY 5 cut(s) 375, 1338, 1741, 2334, 2754
BstZ17I GTATAC 2 cut(s) 2197, 3032
Bsu15I ATCGAT 1 cut(s) 2487
BsuTUI ATCGAT 1 cut(s) 2487
BtgI CCRYGG 1 cut(s) 819
BtsCI GGATG 5 cut(s) 591, 1055, 2018, 2622, 3106
BtsI GCAGTG 1 cut(s) 2094
BtsIMutI CAGTG 5 cut(s) 2094, 2300, 2440, 2605, 2889
Cac8I GCNNGC 4 cut(s) 241, 1939, 2613, 3061
CaiI CAGNNNCTG 2 cut(s) 27, 2909
CciI TCATGA 1 cut(s) 2515
CfoI GCGC 2 cut(s) 115, 411
Cfr13I GGNCC 3 cut(s) 2245, 2872, 3112
ClaI ATCGAT 1 cut(s) 2487
Csp6I GTAC 4 cut(s) 17, 1670, 2122, 3125
CspCI CAANNNNNGTGG 2 cut(s) 585, 620
CviQI GTAC 4 cut(s) 17, 1670, 2122, 3125
DraI TTTAAA 1 cut(s) 2380
DriI GACNNNNNGTC 1 cut(s) 468
Eam1104I CTCTTC 4 cut(s) 779, 2025, 2886, 2967
Eam1105I GACNNNNNGTC 1 cut(s) 468
EarI CTCTTC 4 cut(s) 779, 2025, 2886, 2967
Ecl136II GAGCTC 1 cut(s) 1378
Eco130I CCWWGG 4 cut(s) 603, 819, 856, 1168
Eco24I GRGCYC 2 cut(s) 539, 1380
Eco47I GGWCC 3 cut(s) 2245, 2872, 3112
Eco53kI GAGCTC 1 cut(s) 1378
Eco57I CTGAAG 4 cut(s) 510, 902, 1598, 1715
EcoICRI GAGCTC 1 cut(s) 1378
EcoO109I RGGNCCY 2 cut(s) 2245, 3112
EcoRI GAATTC 2 cut(s) 447, 869
EcoT14I CCWWGG 4 cut(s) 603, 819, 856, 1168
EcoT38I GRGCYC 2 cut(s) 539, 1380
ErhI CCWWGG 4 cut(s) 603, 819, 856, 1168
Esp3I CGTCTC 1 cut(s) 1606
FalI AAGNNNNNCTT 4 cut(s) 840, 872, 2193, 2225
FaqI GGGAC 2 cut(s) 1937, 3125
FbaI TGATCA 1 cut(s) 133
FblI GTMKAC 2 cut(s) 2196, 3031
Fnu4HI GCNGC 9 cut(s) 244, 989, 1072, 1298, 1928, 2429, 2694, 2913, 2916
FokI GGATG 5 cut(s) 578, 1042, 2025, 2609, 3113
FriOI GRGCYC 2 cut(s) 539, 1380
Fsp4HI GCNGC 9 cut(s) 244, 989, 1072, 1298, 1928, 2429, 2694, 2913, 2916
FspBI CTAG 8 cut(s) 566, 888, 998, 1400, 1739, 2387, 2612, 2849
GlaI GCGC 2 cut(s) 114, 410
GluI GCNGC 9 cut(s) 244, 989, 1072, 1298, 1928, 2429, 2694, 2913, 2916
GsaI CCCAGC 1 cut(s) 24
GsuI CTGGAG 5 cut(s) 1641, 1853, 2335, 2682, 2913
HapII CCGG 2 cut(s) 2148, 2314
HhaI GCGC 2 cut(s) 115, 411
Hin1I GRCGYC 1 cut(s) 1599
Hin6I GCGC 2 cut(s) 113, 409
HinP1I GCGC 2 cut(s) 113, 409
HincII GTYRAC 2 cut(s) 457, 1146
HindII GTYRAC 2 cut(s) 457, 1146
HindIII AAGCTT 3 cut(s) 691, 2268, 2413
HpaII CCGG 2 cut(s) 2148, 2314
HphI GGTGA 5 cut(s) 300, 527, 1151, 1850, 3066
Hpy99I CGWCG 1 cut(s) 2012
HpyAV CCTTC 8 cut(s) 59, 243, 475, 534, 609, 1713, 2264, 2471
HpyCH4III ACNGT 3 cut(s) 1106, 2435, 2813
HpyCH4IV ACGT 2 cut(s) 1599, 2565
HpyF10VI GCNNNNNNNGC 7 cut(s) 45, 994, 1306, 1653, 2187, 2885, 2912
HpySE526I ACGT 2 cut(s) 1599, 2565
Hsp92I GRCGYC 1 cut(s) 1599
HspAI GCGC 2 cut(s) 113, 409
Ksp22I TGATCA 1 cut(s) 133
LguI GCTCTTC 1 cut(s) 2886
LmnI GCTCC 4 cut(s) 2354, 2451, 2701, 2911
Lsp1109I GCAGC 9 cut(s) 255, 975, 1083, 1284, 1939, 2415, 2680, 2924, 2927
LweI GCATC 6 cut(s) 262, 492, 600, 1064, 2003, 2732
MaeI CTAG 8 cut(s) 566, 888, 998, 1400, 1739, 2387, 2612, 2849
MaeII ACGT 2 cut(s) 1599, 2565
MaeIII GTNAC 4 cut(s) 1183, 1319, 2546, 2899
MfeI CAATTG 2 cut(s) 223, 2664
MflI RGATCY 5 cut(s) 375, 1338, 1741, 2334, 2754
MhlI GDGCHC 4 cut(s) 539, 806, 1380, 3065
MlyI GAGTC 4 cut(s) 1150, 1272, 2613, 2633
MmeI TCCRAC 3 cut(s) 417, 427, 1990
MroXI GAANNNNTTC 2 cut(s) 613, 1027
MseI TTAA 6 cut(s) 390, 753, 798, 2339, 2379, 2835
MslI CAYNNNNRTG 3 cut(s) 2955, 3017, 3019
MspI CCGG 2 cut(s) 2148, 2314
MunI CAATTG 2 cut(s) 223, 2664
Mva1269I GAATGC 2 cut(s) 115, 2297
MwoI GCNNNNNNNGC 7 cut(s) 45, 994, 1306, 1653, 2187, 2885, 2912
NcoI CCATGG 1 cut(s) 819
NheI GCTAGC 1 cut(s) 2611
NlaIV GGNNCC 5 cut(s) 715, 1845, 2756, 2874, 3113
NmuCI GTSAC 2 cut(s) 2546, 2899
NspI RCATGY 7 cut(s) 436, 835, 1941, 2346, 2404, 2941, 3020
NspV TTCGAA 2 cut(s) 935, 1864
OliI CACNNNNGTG 2 cut(s) 3017, 3019
PaeI GCATGC 1 cut(s) 1941
PagI TCATGA 1 cut(s) 2515
PciI ACATGT 4 cut(s) 432, 2342, 2400, 3016
PciSI GCTCTTC 1 cut(s) 2886
PctI GAATGC 2 cut(s) 115, 2297
PdmI GAANNNNTTC 2 cut(s) 613, 1027
PkrI GCNGC 9 cut(s) 245, 990, 1073, 1299, 1929, 2430, 2695, 2914, 2917
PleI GAGTC 4 cut(s) 1150, 1271, 2613, 2633
PpsI GAGTC 4 cut(s) 1150, 1271, 2613, 2633
PpuMI RGGWCCY 2 cut(s) 2245, 3112
PscI ACATGT 4 cut(s) 432, 2342, 2400, 3016
PsiI TTATAA 2 cut(s) 357, 1101
Psp124BI GAGCTC 1 cut(s) 1380
Psp1406I AACGTT 1 cut(s) 2565
Psp5II RGGWCCY 2 cut(s) 2245, 3112
PspFI CCCAGC 1 cut(s) 20
PspN4I GGNNCC 5 cut(s) 715, 1845, 2756, 2874, 3113
PspPI GGNCC 3 cut(s) 2245, 2872, 3112
PspPPI RGGWCCY 2 cut(s) 2245, 3112
PstI CTGCAG 3 cut(s) 993, 2089, 2914
PstNI CAGNNNCTG 2 cut(s) 27, 2909
PsuI RGATCY 5 cut(s) 375, 1338, 1741, 2334, 2754
RsaI GTAC 4 cut(s) 18, 1671, 2123, 3126
RsaNI GTAC 4 cut(s) 17, 1670, 2122, 3125
RseI CAYNNNNRTG 3 cut(s) 2955, 3017, 3019
SacI GAGCTC 1 cut(s) 1380
SapI GCTCTTC 1 cut(s) 2886
SaqAI TTAA 6 cut(s) 390, 753, 798, 2339, 2379, 2835
SatI GCNGC 9 cut(s) 244, 989, 1072, 1298, 1928, 2429, 2694, 2913, 2916
Sau96I GGNCC 3 cut(s) 2245, 2872, 3112
SchI GAGTC 4 cut(s) 1150, 1272, 2613, 2633
SduI GDGCHC 4 cut(s) 539, 806, 1380, 3065
SfaNI GCATC 6 cut(s) 262, 492, 600, 1064, 2003, 2732
SfcI CTRYAG 5 cut(s) 989, 1131, 1784, 2085, 2910
SfuI TTCGAA 2 cut(s) 935, 1864
SinI GGWCC 3 cut(s) 2245, 2872, 3112
SmiMI CAYNNNNRTG 3 cut(s) 2955, 3017, 3019
SmlI CTYRAG 2 cut(s) 2214, 3044
SmoI CTYRAG 2 cut(s) 2214, 3044
SphI GCATGC 1 cut(s) 1941
SsiI CCGC 4 cut(s) 505, 1875, 1908, 2181
SspI AATATT 1 cut(s) 1084
SspMI CTAG 8 cut(s) 566, 888, 998, 1400, 1739, 2387, 2612, 2849
SstI GAGCTC 1 cut(s) 1380
StyI CCWWGG 4 cut(s) 603, 819, 856, 1168
TaaI ACNGT 3 cut(s) 1106, 2435, 2813
TaiI ACGT 2 cut(s) 1602, 2568
TaqI TCGA 5 cut(s) 935, 1010, 1864, 2487, 2637
TatI WGTACW 2 cut(s) 1669, 3124
Tru1I TTAA 6 cut(s) 390, 753, 798, 2339, 2379, 2835
Tru9I TTAA 6 cut(s) 390, 753, 798, 2339, 2379, 2835
TscAI CASTG 5 cut(s) 2094, 2307, 2440, 2605, 2896
TseFI GTSAC 2 cut(s) 2546, 2899
TseI GCWGC 9 cut(s) 243, 988, 1071, 1297, 1927, 2428, 2693, 2912, 2915
Tsp45I GTSAC 2 cut(s) 2546, 2899
TspGWI ACGGA 2 cut(s) 2372, 2424
TspRI CASTG 5 cut(s) 2094, 2307, 2440, 2605, 2896
VpaK11BI GGWCC 3 cut(s) 2245, 2872, 3112
XapI RAATTY 7 cut(s) 152, 447, 519, 869, 882, 1626, 1661
XbaI TCTAGA 2 cut(s) 565, 1738
XceI RCATGY 7 cut(s) 436, 835, 1941, 2346, 2404, 2941, 3020
XmiI GTMKAC 2 cut(s) 2196, 3031
XmnI GAANNNNTTC 2 cut(s) 613, 1027
XspI CTAG 8 cut(s) 566, 888, 998, 1400, 1739, 2387, 2612, 2849
ZraI GACGTC 1 cut(s) 1600
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.