Belongs to the protein kinase superfamily. Ser Thr protein kinase family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc65I | GGTACC | 1 cut(s) | 772 |
| AccB1I | GGYRCC | 1 cut(s) | 772 |
| AciI | CCGC | 4 cut(s) | 41, 73, 1007, 1077 |
| AclI | AACGTT | 1 cut(s) | 1494 |
| AclWI | GGATC | 1 cut(s) | 1796 |
| AcoI | YGGCCR | 2 cut(s) | 384, 1770 |
| AcsI | RAATTY | 7 cut(s) | 15, 304, 555, 590, 699, 1921, 2013 |
| AcuI | CTGAAG | 4 cut(s) | 78, 527, 582, 644 |
| AfaI | GTAC | 4 cut(s) | 198, 603, 774, 1127 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 173, 400, 496, 782, 1042 |
| AflIII | ACRYGT | 1 cut(s) | 1271 |
| AjnI | CCWGG | 1 cut(s) | 1777 |
| AleI | CACNNNNGTG | 1 cut(s) | 1943 |
| Alw26I | GTCTC | 1 cut(s) | 712 |
| AlwI | GGATC | 1 cut(s) | 1796 |
| AoxI | GGCC | 4 cut(s) | 247, 384, 1770, 2090 |
| ApeKI | GCWGC | 3 cut(s) | 856, 1357, 1622 |
| ApoI | RAATTY | 7 cut(s) | 15, 304, 555, 590, 699, 1921, 2013 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 686 |
| Asp718I | GGTACC | 1 cut(s) | 772 |
| AspS9I | GGNCC | 3 cut(s) | 281, 1174, 2029 |
| AsuHPI | GGTGA | 2 cut(s) | 1541, 1992 |
| AsuII | TTCGAA | 1 cut(s) | 793 |
| AvaII | GGWCC | 3 cut(s) | 281, 1174, 2029 |
| BalI | TGGCCA | 2 cut(s) | 386, 1772 |
| BanI | GGYRCC | 1 cut(s) | 772 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 616, 648 |
| BauI | CACGAG | 1 cut(s) | 1521 |
| BbsI | GAAGAC | 1 cut(s) | 650 |
| BbvI | GCAGC | 3 cut(s) | 868, 1344, 1609 |
| BccI | CCATC | 5 cut(s) | 812, 881, 1660, 1704, 1762 |
| BciT130I | CCWGG | 1 cut(s) | 1779 |
| BclI | TGATCA | 1 cut(s) | 1141 |
| BcoDI | GTCTC | 1 cut(s) | 712 |
| BfaI | CTAG | 4 cut(s) | 245, 524, 668, 1316 |
| BfmI | CTRYAG | 4 cut(s) | 453, 713, 854, 1014 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 81 |
| BglII | AGATCT | 1 cut(s) | 670 |
| BisI | GCNGC | 3 cut(s) | 857, 1358, 1623 |
| BlpI | GCTNAGC | 2 cut(s) | 1647, 1691 |
| BlsI | GCNGC | 3 cut(s) | 858, 1359, 1624 |
| Bme1390I | CCNGG | 1 cut(s) | 1779 |
| Bme18I | GGWCC | 3 cut(s) | 281, 1174, 2029 |
| BmgT120I | GGNCC | 3 cut(s) | 281, 1174, 2029 |
| BmiI | GGNNCC | 3 cut(s) | 283, 774, 1120 |
| BmrFI | CCNGG | 1 cut(s) | 1779 |
| BmsI | GCATC | 6 cut(s) | 60, 133, 168, 218, 1779, 2047 |
| BoxI | GACNNNNGTC | 1 cut(s) | 2079 |
| BpiI | GAAGAC | 1 cut(s) | 650 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 89, 121 |
| BpmI | CTGGAG | 5 cut(s) | 332, 422, 1000, 1264, 1611 |
| Bpu1102I | GCTNAGC | 2 cut(s) | 1647, 1691 |
| Bpu14I | TTCGAA | 1 cut(s) | 793 |
| BpuEI | CTTGAG | 3 cut(s) | 676, 782, 1991 |
| BsaAI | YACGTR | 1 cut(s) | 601 |
| BsaJI | CCNNGG | 5 cut(s) | 171, 204, 490, 1563, 2003 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 173, 400, 496, 782, 1042 |
| Bse118I | RCCGGY | 1 cut(s) | 80 |
| Bse1I | ACTGG | 5 cut(s) | 20, 863, 983, 1078, 2071 |
| Bse3DI | GCAATG | 2 cut(s) | 235, 1231 |
| BseBI | CCWGG | 1 cut(s) | 1779 |
| BseDI | CCNNGG | 5 cut(s) | 171, 204, 490, 1563, 2003 |
| BseGI | GGATG | 5 cut(s) | 159, 209, 823, 947, 1672 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 173, 400, 496, 782, 1042 |
| BseMI | GCAATG | 2 cut(s) | 235, 1231 |
| BseMII | CTCAG | 6 cut(s) | 38, 358, 407, 1223, 1638, 1682 |
| BseNI | ACTGG | 5 cut(s) | 20, 863, 983, 1078, 2071 |
| BseRI | GAGGAG | 2 cut(s) | 1364, 2039 |
| BseXI | GCAGC | 3 cut(s) | 868, 1344, 1609 |
| BseYI | CCCAGC | 1 cut(s) | 321 |
| BshFI | GGCC | 4 cut(s) | 249, 386, 1772, 2092 |
| BshNI | GGYRCC | 1 cut(s) | 772 |
| BsiSI | CCGG | 2 cut(s) | 81, 1243 |
| BslFI | GGGAC | 3 cut(s) | 267, 855, 866 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 173, 400, 496, 782, 1042 |
| BsmAI | GTCTC | 1 cut(s) | 712 |
| BsmFI | GGGAC | 3 cut(s) | 267, 855, 866 |
| BsmI | GAATGC | 1 cut(s) | 1226 |
| BsnI | GGCC | 4 cut(s) | 249, 386, 1772, 2092 |
| Bsp119I | TTCGAA | 1 cut(s) | 793 |
| Bsp1407I | TGTACA | 1 cut(s) | 1125 |
| Bsp143I | GATC | 3 cut(s) | 670, 1141, 1801 |
| Bsp1720I | GCTNAGC | 2 cut(s) | 1647, 1691 |
| BspACI | CCGC | 4 cut(s) | 41, 73, 1007, 1077 |
| BspANI | GGCC | 4 cut(s) | 249, 386, 1772, 2092 |
| BspCNI | CTCAG | 6 cut(s) | 39, 357, 408, 1224, 1639, 1683 |
| BspLI | GGNNCC | 3 cut(s) | 283, 774, 1120 |
| BspMAI | CTGCAG | 1 cut(s) | 858 |
| BspPI | GGATC | 1 cut(s) | 1796 |
| BspT104I | TTCGAA | 1 cut(s) | 793 |
| BspT107I | GGYRCC | 1 cut(s) | 772 |
| BsrDI | GCAATG | 2 cut(s) | 235, 1231 |
| BsrFI | RCCGGY | 1 cut(s) | 80 |
| BsrGI | TGTACA | 1 cut(s) | 1125 |
| BsrI | ACTGG | 5 cut(s) | 20, 863, 983, 1078, 2071 |
| BssAI | RCCGGY | 1 cut(s) | 80 |
| BssECI | CCNNGG | 5 cut(s) | 171, 204, 490, 1563, 2003 |
| BssMI | GATC | 3 cut(s) | 670, 1141, 1801 |
| BssSI | CACGAG | 1 cut(s) | 1521 |
| BssT1I | CCWWGG | 4 cut(s) | 171, 204, 490, 2003 |
| Bst2BI | CACGAG | 1 cut(s) | 1521 |
| Bst2UI | CCWGG | 1 cut(s) | 1779 |
| Bst4CI | ACNGT | 4 cut(s) | 196, 1018, 1475, 1594 |
| Bst6I | CTCTTC | 2 cut(s) | 1576, 1815 |
| BstAUI | TGTACA | 1 cut(s) | 1125 |
| BstBAI | YACGTR | 1 cut(s) | 601 |
| BstBI | TTCGAA | 1 cut(s) | 793 |
| BstC8I | GCNNGC | 1 cut(s) | 1987 |
| BstDEI | CTNAG | 7 cut(s) | 47, 344, 416, 1203, 1232, 1647, 1691 |
| BstDSI | CCRYGG | 1 cut(s) | 1563 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 1040 |
| BstF5I | GGATG | 5 cut(s) | 159, 209, 823, 947, 1672 |
| BstKTI | GATC | 3 cut(s) | 673, 1144, 1804 |
| BstMAI | GTCTC | 1 cut(s) | 712 |
| BstMBI | GATC | 3 cut(s) | 670, 1141, 1801 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 81, 354, 1116, 1643, 1687 |
| BstNI | CCWGG | 1 cut(s) | 1779 |
| BstNSI | RCATGY | 2 cut(s) | 1275, 1989 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 2079 |
| BstSCI | CCNGG | 1 cut(s) | 1777 |
| BstSFI | CTRYAG | 4 cut(s) | 453, 713, 854, 1014 |
| BstSNI | TACGTA | 1 cut(s) | 601 |
| BstV1I | GCAGC | 3 cut(s) | 868, 1344, 1609 |
| BstV2I | GAAGAC | 1 cut(s) | 650 |
| BstX2I | RGATCY | 2 cut(s) | 670, 1801 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 220 |
| BstYI | RGATCY | 2 cut(s) | 670, 1801 |
| BsuRI | GGCC | 4 cut(s) | 249, 386, 1772, 2092 |
| BtgI | CCRYGG | 1 cut(s) | 1563 |
| BtsCI | GGATG | 5 cut(s) | 159, 209, 823, 947, 1672 |
| BtsIMutI | CAGTG | 6 cut(s) | 192, 870, 1023, 1229, 1471, 2064 |
| Cac8I | GCNNGC | 1 cut(s) | 1987 |
| Cfr10I | RCCGGY | 1 cut(s) | 80 |
| Cfr13I | GGNCC | 3 cut(s) | 281, 1174, 2029 |
| Csp6I | GTAC | 4 cut(s) | 197, 602, 773, 1126 |
| CspCI | CAANNNNNGTGG | 4 cut(s) | 153, 188, 817, 852 |
| CviAII | CATG | 6 cut(s) | 902, 1213, 1272, 1480, 1513, 1986 |
| CviQI | GTAC | 4 cut(s) | 197, 602, 773, 1126 |
| DdeI | CTNAG | 7 cut(s) | 47, 344, 416, 1203, 1232, 1647, 1691 |
| DpnI | GATC | 3 cut(s) | 672, 1143, 1803 |
| DpnII | GATC | 3 cut(s) | 670, 1141, 1801 |
| DraI | TTTAAA | 1 cut(s) | 1735 |
| EaeI | YGGCCR | 2 cut(s) | 384, 1770 |
| Eam1104I | CTCTTC | 2 cut(s) | 1576, 1815 |
| EarI | CTCTTC | 2 cut(s) | 1576, 1815 |
| EciI | GGCGGA | 1 cut(s) | 1092 |
| Eco105I | TACGTA | 1 cut(s) | 601 |
| Eco130I | CCWWGG | 4 cut(s) | 171, 204, 490, 2003 |
| Eco47I | GGWCC | 3 cut(s) | 281, 1174, 2029 |
| Eco57I | CTGAAG | 4 cut(s) | 78, 527, 582, 644 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 1040 |
| EcoO109I | RGGNCCY | 1 cut(s) | 1174 |
| EcoRI | GAATTC | 3 cut(s) | 15, 304, 590 |
| EcoRII | CCWGG | 1 cut(s) | 1777 |
| EcoT14I | CCWWGG | 4 cut(s) | 171, 204, 490, 2003 |
| ErhI | CCWWGG | 4 cut(s) | 171, 204, 490, 2003 |
| FaeI | CATG | 6 cut(s) | 905, 1216, 1275, 1483, 1516, 1989 |
| FaqI | GGGAC | 3 cut(s) | 267, 855, 866 |
| FatI | CATG | 6 cut(s) | 901, 1212, 1271, 1479, 1512, 1985 |
| FbaI | TGATCA | 1 cut(s) | 1141 |
| Fnu4HI | GCNGC | 3 cut(s) | 857, 1358, 1623 |
| FokI | GGATG | 5 cut(s) | 146, 196, 830, 954, 1679 |
| Fsp4HI | GCNGC | 3 cut(s) | 857, 1358, 1623 |
| FspBI | CTAG | 4 cut(s) | 245, 524, 668, 1316 |
| GluI | GCNGC | 3 cut(s) | 857, 1358, 1623 |
| GsaI | CCCAGC | 1 cut(s) | 325 |
| GsuI | CTGGAG | 5 cut(s) | 332, 422, 1000, 1264, 1611 |
| HaeIII | GGCC | 4 cut(s) | 249, 386, 1772, 2092 |
| HapII | CCGG | 2 cut(s) | 81, 1243 |
| Hin1II | CATG | 6 cut(s) | 905, 1216, 1275, 1483, 1516, 1989 |
| HincII | GTYRAC | 2 cut(s) | 25, 2074 |
| HindII | GTYRAC | 2 cut(s) | 25, 2074 |
| HindIII | AAGCTT | 1 cut(s) | 259 |
| HpaII | CCGG | 2 cut(s) | 81, 1243 |
| HphI | GGTGA | 2 cut(s) | 1541, 1992 |
| Hpy166II | GTNNAC | 7 cut(s) | 25, 921, 1126, 1457, 1861, 2032, 2074 |
| Hpy8I | GTNNAC | 7 cut(s) | 25, 921, 1126, 1457, 1861, 2032, 2074 |
| Hpy99I | CGWCG | 1 cut(s) | 1727 |
| HpyAV | CCTTC | 7 cut(s) | 43, 177, 502, 642, 776, 1193, 1400 |
| HpyCH4III | ACNGT | 4 cut(s) | 196, 1018, 1475, 1594 |
| HpyCH4IV | ACGT | 2 cut(s) | 600, 1494 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 81, 354, 1116, 1643, 1687 |
| HpyF3I | CTNAG | 7 cut(s) | 47, 344, 416, 1203, 1232, 1647, 1691 |
| HpySE526I | ACGT | 2 cut(s) | 600, 1494 |
| Hsp92II | CATG | 6 cut(s) | 905, 1216, 1275, 1483, 1516, 1989 |
| KpnI | GGTACC | 1 cut(s) | 776 |
| Ksp22I | TGATCA | 1 cut(s) | 1141 |
| Kzo9I | GATC | 3 cut(s) | 670, 1141, 1801 |
| LmnI | GCTCC | 4 cut(s) | 1124, 1196, 1283, 1630 |
| Lsp1109I | GCAGC | 3 cut(s) | 868, 1344, 1609 |
| LweI | GCATC | 6 cut(s) | 60, 133, 168, 218, 1779, 2047 |
| MaeI | CTAG | 4 cut(s) | 245, 524, 668, 1316 |
| MaeII | ACGT | 2 cut(s) | 600, 1494 |
| MaeIII | GTNAC | 3 cut(s) | 422, 1475, 1874 |
| MalI | GATC | 3 cut(s) | 672, 1143, 1803 |
| MboI | GATC | 3 cut(s) | 670, 1141, 1801 |
| MfeI | CAATTG | 3 cut(s) | 276, 473, 1611 |
| MflI | RGATCY | 2 cut(s) | 670, 1801 |
| MlsI | TGGCCA | 2 cut(s) | 386, 1772 |
| MluNI | TGGCCA | 2 cut(s) | 386, 1772 |
| MlyI | GAGTC | 5 cut(s) | 187, 472, 795, 1542, 1562 |
| MmeI | TCCRAC | 2 cut(s) | 237, 1988 |
| Mox20I | TGGCCA | 2 cut(s) | 386, 1772 |
| MroXI | GAANNNNTTC | 1 cut(s) | 686 |
| MscI | TGGCCA | 2 cut(s) | 386, 1772 |
| MseI | TTAA | 4 cut(s) | 129, 1268, 1734, 1925 |
| MslI | CAYNNNNRTG | 1 cut(s) | 1943 |
| Msp20I | TGGCCA | 2 cut(s) | 386, 1772 |
| MspI | CCGG | 2 cut(s) | 81, 1243 |
| MspR9I | CCNGG | 1 cut(s) | 1779 |
| MunI | CAATTG | 3 cut(s) | 276, 473, 1611 |
| Mva1269I | GAATGC | 1 cut(s) | 1226 |
| MvaI | CCWGG | 1 cut(s) | 1779 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 81, 354, 1116, 1643, 1687 |
| NdeII | GATC | 3 cut(s) | 670, 1141, 1801 |
| NlaIII | CATG | 6 cut(s) | 905, 1216, 1275, 1483, 1516, 1989 |
| NlaIV | GGNNCC | 3 cut(s) | 283, 774, 1120 |
| NmeAIII | GCCGAG | 1 cut(s) | 1512 |
| NmuCI | GTSAC | 1 cut(s) | 1475 |
| NspI | RCATGY | 2 cut(s) | 1275, 1989 |
| NspV | TTCGAA | 1 cut(s) | 793 |
| OliI | CACNNNNGTG | 1 cut(s) | 1943 |
| PaeI | GCATGC | 1 cut(s) | 1989 |
| PciI | ACATGT | 1 cut(s) | 1271 |
| PctI | GAATGC | 1 cut(s) | 1226 |
| PdmI | GAANNNNTTC | 1 cut(s) | 686 |
| PfeI | GAWTC | 5 cut(s) | 610, 1080, 1135, 1252, 1997 |
| PkrI | GCNGC | 3 cut(s) | 858, 1359, 1624 |
| PleI | GAGTC | 5 cut(s) | 186, 472, 794, 1542, 1562 |
| PpsI | GAGTC | 5 cut(s) | 186, 472, 794, 1542, 1562 |
| Ppu21I | YACGTR | 1 cut(s) | 601 |
| PpuMI | RGGWCCY | 1 cut(s) | 1174 |
| PscI | ACATGT | 1 cut(s) | 1271 |
| PshAI | GACNNNNGTC | 1 cut(s) | 2079 |
| Psp1406I | AACGTT | 1 cut(s) | 1494 |
| Psp5II | RGGWCCY | 1 cut(s) | 1174 |
| Psp6I | CCWGG | 1 cut(s) | 1777 |
| PspFI | CCCAGC | 1 cut(s) | 321 |
| PspGI | CCWGG | 1 cut(s) | 1777 |
| PspN4I | GGNNCC | 3 cut(s) | 283, 774, 1120 |
| PspPI | GGNCC | 3 cut(s) | 281, 1174, 2029 |
| PspPPI | RGGWCCY | 1 cut(s) | 1174 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 843, 875 |
| PstI | CTGCAG | 1 cut(s) | 858 |
| PsuI | RGATCY | 2 cut(s) | 670, 1801 |
| RsaI | GTAC | 4 cut(s) | 198, 603, 774, 1127 |
| RsaNI | GTAC | 4 cut(s) | 197, 602, 773, 1126 |
| RseI | CAYNNNNRTG | 1 cut(s) | 1943 |
| SaqAI | TTAA | 4 cut(s) | 129, 1268, 1734, 1925 |
| SatI | GCNGC | 3 cut(s) | 857, 1358, 1623 |
| Sau3AI | GATC | 3 cut(s) | 670, 1141, 1801 |
| Sau96I | GGNCC | 3 cut(s) | 281, 1174, 2029 |
| SchI | GAGTC | 5 cut(s) | 187, 472, 795, 1542, 1562 |
| ScrFI | CCNGG | 1 cut(s) | 1779 |
| SfaNI | GCATC | 6 cut(s) | 60, 133, 168, 218, 1779, 2047 |
| SfcI | CTRYAG | 4 cut(s) | 453, 713, 854, 1014 |
| SfuI | TTCGAA | 1 cut(s) | 793 |
| SinI | GGWCC | 3 cut(s) | 281, 1174, 2029 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 1943 |
| SmlI | CTYRAG | 3 cut(s) | 691, 761, 1970 |
| SmoI | CTYRAG | 3 cut(s) | 691, 761, 1970 |
| SnaBI | TACGTA | 1 cut(s) | 601 |
| SphI | GCATGC | 1 cut(s) | 1989 |
| SsiI | CCGC | 4 cut(s) | 41, 73, 1007, 1077 |
| SspI | AATATT | 2 cut(s) | 810, 1408 |
| SspMI | CTAG | 4 cut(s) | 245, 524, 668, 1316 |
| StyD4I | CCNGG | 1 cut(s) | 1777 |
| StyI | CCWWGG | 4 cut(s) | 171, 204, 490, 2003 |
| TaaI | ACNGT | 4 cut(s) | 196, 1018, 1475, 1594 |
| TaiI | ACGT | 2 cut(s) | 603, 1497 |
| TaqI | TCGA | 5 cut(s) | 619, 677, 793, 1722, 1890 |
| TatI | WGTACW | 2 cut(s) | 196, 1125 |
| TfiI | GAWTC | 5 cut(s) | 610, 1080, 1135, 1252, 1997 |
| Tru1I | TTAA | 4 cut(s) | 129, 1268, 1734, 1925 |
| Tru9I | TTAA | 4 cut(s) | 129, 1268, 1734, 1925 |
| TscAI | CASTG | 6 cut(s) | 199, 870, 1023, 1236, 1478, 2071 |
| TseFI | GTSAC | 1 cut(s) | 1475 |
| TseI | GCWGC | 3 cut(s) | 856, 1357, 1622 |
| Tsp45I | GTSAC | 1 cut(s) | 1475 |
| TspGWI | ACGGA | 2 cut(s) | 1111, 1580 |
| TspRI | CASTG | 6 cut(s) | 199, 870, 1023, 1236, 1478, 2071 |
| VpaK11BI | GGWCC | 3 cut(s) | 281, 1174, 2029 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 1040 |
| XapI | RAATTY | 7 cut(s) | 15, 304, 555, 590, 699, 1921, 2013 |
| XbaI | TCTAGA | 1 cut(s) | 667 |
| XceI | RCATGY | 2 cut(s) | 1275, 1989 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 220 |
| XmnI | GAANNNNTTC | 1 cut(s) | 686 |
| XspI | CTAG | 4 cut(s) | 245, 524, 668, 1316 |