FvH4_7g24151
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0000248
Physical Location & Seq
Forward (+)
18684305 .. 18687856
3552 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g24151.t1

Sequence Viewer

Length: 3093 bp
ATGACCACCCTTTTCCTTCTCACCAATCTTTTCCAATCGACCGTCATTGCAAACGCCTCGAGCAATGAAACTGATCGATTCGCGCTGCTCAACCTCAAGGAATCCATAGTCAGTGACCCACAAGGGTTGTTGAACTCATGGAACGAATCCATTCACTTCTGCAAATGGCAAGGAATTAATTGCAGCAGACGACATCAAAGAGTAACAGCCTTGAACCTACCAGCTGCTGAGTTGCATGGAACTATATCGCCATACATTGGCAACCTCTCCTTCCTCAGGTTCATCAACCTTGGAAGTAACAACTTCTCTGGCAAGATTCCGCAACAAGTTGATCATTTGTTCCGACTGCAATATCTAAATCTCAGTGCCAACATGTTGGAGGGGGGAATTCCATTAAACCTGACCTTTTGTCCGGAACTGAGAGTACTAAGTATTGGACAAAACCGCCTTACCGGCAAGATTCCTTCAGAGATTGTCTCGTTGACGAAGCTTGTGCATTTTAACGTTGACAGAAACAATTTGACAGGAGGCATCCCACCTTTCTTGGGAAATCTTTCATCAATCGGAAGACTTTCATTGGGAGGGAACCCATTAGGGGGCACAGTTCCTGAGGAAATAGGACGACTGAGAAACCTCTGGTTTTTTAGTATTAATTCCGGTAATCTCACTGGTATGATCCCTCCCTCCCTTTTTAACATGTCATCTCTGGAAGTCATTTCATATACGTTTAATGAGTTTAAGGGCAGTATTCCGCCTGATATAGGCCTAAACATGCCTAATCTCCAAGAAGTGTACCTCGCCGGAAATGATTTCTCTGGAGAAATCCCAGCTTCATTTTCCAATGCTTCTCAACTTAAAAAGTTTGATATCTGGGACAATATGTTTGTAGGGGATGTTCCCACAAGTTTTGGAAATCTTCCTAATCTCCATTGGCTCAACCTCGGTTTCAATCATCTAGGAAGTAATTCATATAATGATTTGGACTTCATAACATCCCTGACCAATTGCAGCAGTCTGAAGATGCTTTCTTTGAGTGTCAACAAATTTGGAGGTGTTTTACCCAACTCCATAGCCAATTTCTCAACCAATCTTTCTCAACTTTACCTTGAGAGCAATCAAATAGGGGGAACAATTCCTGAGACATTAGGGAATCTCAACAATTTAATAGCCTTTGCCCTGGATGTTAATCTGTTCACAGGTATCATTCCAGCTTCTTTTGGGAAGTTACAAAAGCTGCAATTATTATCTTTATCTGCAAATAGATTATCAGGACGAATCCCTTCTTCCCTAGGAAACCTCACCCAACTGTTTGAATTCAACGTATCACAAAATGAGTTAGAAGGAAGCATTCCTCCAAATATTGGTAACTGCCAACTTCTGCAGGAGATGGATTTATCAGACAATAAGCTTAGTGGAGATATACCATCACAGGTCATTGGTCTGTCCACACTTAACTATCTCAACTGGTCACATAACTCACTAACCGGCAGTCTGCCTGTGGAAGTGGGTAAGCTGAAAAGTGCCAGTACACTAGACCTCTCTTATAATAATTTGACTGGAGGAATTCCGGAAAACATTAGAAGCTGTCTGAGCCTTGAATTTCTCCACTTACAAGGGAATCTTTTACAAGGTATGATACCTTCTTCTTTGGCTTCCTTGAAGGGTCTTCAGATCCTAGATCTTTCGGGAAACAAATTGTCAAGCCAAATTCCAAAGGATATGCAGAGACTTCAATTTATGATATATTTGAATCTTTCATTCAATAATCTGGAGGGTGTGGTACCCCAAGAGGGAGTGTTTCAAAACATCAGTGCAATATCATTGGATGGAAATACAAAACTCTGTGGTGGTGTTCCGGAACTGCAACTACCACCATGCACCATCAAAGTATCAAAGCAAAGAAAGTTGCAAGGTTTCAAACTGAAGCTCACTATTTCTATTGTCGCTGGATGCTCCGTTTTGTTTGCTCTCATGTTAGCTGTTTACTGGAGGAGGAAAACTCAGAAGACGAAACCAGTATCTGAAGTGGCATCAAACAACTCTTTTTCAAAGGTTTCATACCAGACACTTCATCAAGCTACTGGCGGATTCTCTTCGAGCAATCAAATTGGATCAGGCGGGTTTAGCTCTGTCTACAAAGGGATTATTGATGAGCTAGAAAACAATGCAGTTGCCATAAAGGTCCTGAACCTTCAACAGAAAGGTGCTTCCAAGAGCTTTGTGGCAGAATGCAATGCACTAAGAAGTATCAGACACAGGAATCTTGTGAAGATTTTAACATGTTGCTCCAGCACAGATTACAATGGTAATGACTTCAAAGCTCTAGTTTTTGAGTATATGTCAAATGGAAGTTTAGAGGAGTGGATGCATAGAGAAAATCAATCAAGGAGTTTGAACCTTCTTCAAAATCTGAATATTGCTTTTGATGTGGCTTCTGCTGTGTGTTATCTTCATGACCATTGTGAACCACAAATTATCCACCGTGACTTGAAGCCAAGCAACGTTCTTCTGGACGATGAGATAGTTGCTCGTGTTAGTGATTTTGGGTTGGCAAGACTAATCCCAACGACCGCAGACTCCTCTGAAAATCAAAGTAGCACAGTTGGGATAAAGGGAACAATTGGCTATGCAGCTCCAGAGTATGCAGTTGGTGTTGAGCCATCAGCACAAGGGGATGTATATAGTTACGGGATACTTGTGCTGCAATTGTTCACGGGAAGAAGACCCACTGATGAAATGTTTGTAGACGGTTTCAATATCCATACTTTTGTTAAGACGGGCATACAAGGAAGGCTTATGGAAATTGTAGATCCTACTCTTATTACCACTTTAGAAGAAGAGACTGCAATCTCAACAACAAACAATGATATCGAAACTGATGGAGGCAACATTGACAATCAGAATTTAAACAGGATGGACAGTTACGTGGGGAAGTGCATACTTCCAACCCTTAAGATTGGACTTGCATGCTCGGAAGAATCACCAAGGAATAGGATTTCTATGGAGGAGGTCCTCAAGGAGCTACACCATGTGAAAATTGCTTACACTGCCATTGGTGCTGACATCCGTCGAGAGAGGCCAACAAGAAGCTAA

Protein Analysis

1031

Amino Acids

112.86

Weight (kDa)

6.03

Isoelectric Point (pI)

38.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 23 - 62 1.6e-09 Leucine rich repeat N-terminal domain
LRR_14 PF23598 83 - 315 8.5e-13 Leucine-rich repeat region
LRR_14 PF23598 330 - 444 1.9e-06 Leucine-rich repeat region
LRR_8 PF13855 363 - 422 1.9e-07 Leucine rich repeat
LRR_14 PF23598 452 - 590 1.7e-09 Leucine-rich repeat region
LRR_8 PF13855 460 - 518 4.1e-06 Leucine rich repeat
LRR_8 PF13855 532 - 590 2e-06 Leucine rich repeat
Pkinase PF00069 698 - 912 1.4e-39 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 700 - 929 4.3e-41 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000248)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g31390 FvH4_5g31630 FvH4_5g31631 FvH4_7g24151 FvH4_7g24160 FvH4_7g24240 FvH4_7g24242 FvH4_7g24243 FvH4_7g24510 FvH4_7g24920 FvH4_7g24920 FvH4_7g24920 FvH4_7g29910
malus_domestica MD01G1131800.v1.1 MD01G1150000.v1.1 MD01G1151300.v1.1
prunus_persica Prupe.2G248300_v2.0.a1
pyrus_communis pycom01g16800
rosa_chinensis RchiOBHm_Chr1g0339821 RchiOBHm_Chr1g0345151 RchiOBHm_Chr1g0346851 RchiOBHm_Chr1g0351981 RchiOBHm_Chr1g0369731 RchiOBHm_Chr1g0369841 RchiOBHm_Chr1g0369861 RchiOBHm_Chr1g0369931 RchiOBHm_Chr1g0369961 RchiOBHm_Chr1g0370261 RchiOBHm_Chr1g0370281 RchiOBHm_Chr5g0076391 RchiOBHm_Chr6g0248051 RchiOBHm_Chr6g0248071
rosa_laevigata RLG00000002548 RLG00000013479 RLG00000027042 RLG00000027045 RLG00000027047 RLG00000027060 RLG00000027063 RLG00000027082 RLG00000027084 RLG00000027087 RLG00000027089 RLG00000027090 RLG00000027091 RLG00000027092 RLG00000027094 RLG00000027097 RLG00000027100 RLG00000027101 RLG00000027111 RLG00000028769 RLG00000028851 RLG00000029244 RLG00000030021
rosa_multiflora Rmu_co7968444.1_g000001 Rmu_co8060564.1_g000001 Rmu_co8098766.1_g000001 Rmu_co8172886.1_g000001 Rmu_co8291893.1_g000001 Rmu_co8327887.1_g000001 Rmu_co8406981.1_g000001 Rmu_co8449189.1_g000001 Rmu_co8470871.1_g000001 Rmu_sc0000756.1_g000006 Rmu_sc0001154.1_g000028 Rmu_sc0001565.1_g000055 Rmu_sc0001657.1_g000002 Rmu_sc0002095.1_g000014 Rmu_sc0002147.1_g000001 Rmu_sc0002705.1_g000004 Rmu_sc0002705.1_g000005 Rmu_sc0004087.1_g000025 Rmu_sc0005500.1_g000022 Rmu_sc0006707.1_g000005 Rmu_sc0006707.1_g000009 Rmu_sc0008140.1_g000012 Rmu_sc0008140.1_g000019 Rmu_sc0016176.1_g000001 Rmu_sc0016906.1_g000006 Rmu_sc0028980.1_g000001 Rmu_sc0041369.1_g000001
rosa_roxburghii Rroxscaffold_3G00232610 Rroxscaffold_4G00286690 Rroxscaffold_4G00286920 Rroxscaffold_4G00286930 Rroxscaffold_4G00286960 Rroxscaffold_4G00286980 Rroxscaffold_4G00287030 Rroxscaffold_4G00287040 Rroxscaffold_4G00287070 Rroxscaffold_4G00287090 Rroxscaffold_4G00287330 Rroxscaffold_4G00287430 Rroxscaffold_4G00303380 Rroxscaffold_4G00303400 Rroxscaffold_4G00303410 Rroxscaffold_4G00308100 Rroxscaffold_4G00308480 Rroxscaffold_4G00313700 Rroxscaffold_4G00322910 Rroxscaffold_7G00190680 Rroxscaffold_7G00193490
rosa_rugosa Rorug01G0032200 Rorug01G0139400.1 Rorug01G0139600.1 Rorug01G0139700.1 Rorug01G0184400 Rorug01G0351900 Rorug01G0351900 Rorug01G0352600 Rorug01G0352600 Rorug01G0352800 Rorug01G0355300 Rorug01G0355400 Rorug01G0356700.1 Rorug01G0356800 Rorug06G0090600 Rorug07G0276100
rosa_samantha Rh1AG201900 Rh1AG361300 Rh1AG365100 Rh6AG204000
rosa_wichuraiana Rw0G015480 Rw1G007000 Rw1G008190 Rw1G012880 Rw1G012990 Rw1G016950 Rw1G031720 Rw1G031800 Rw1G031820 Rw1G032020 Rw1G032180 Rw6G017720 Rw7G035600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1545
Acc65I GGTACC 1 cut(s) 1780
AccB1I GGYRCC 1 cut(s) 1780
AccB7I CCANNNNNTGG 2 cut(s) 257, 1361
AccI GTMKAC 2 cut(s) 2133, 2745
AccII CGCG 1 cut(s) 83
AccIII TCCGGA 3 cut(s) 412, 1567, 1855
AciI CCGC 6 cut(s) 320, 445, 752, 2085, 2118, 2571
AclI AACGTT 2 cut(s) 504, 2502
AclWI GGATC 4 cut(s) 670, 1666, 2119, 2804
AcsI RAATTY 7 cut(s) 387, 1043, 1313, 1563, 1598, 1707, 2902
AcuI CTGAAG 5 cut(s) 450, 1037, 1652, 1943, 2043
AdeI CACNNNGTG 1 cut(s) 3031
AfaI GTAC 4 cut(s) 426, 794, 1528, 1782
AfiI CCNNNNNNNGG 8 cut(s) 257, 276, 545, 595, 596, 761, 1361, 1790
AflII CTTAAG 1 cut(s) 2951
AflIII ACRYGT 3 cut(s) 372, 696, 2279
AhdI GACNNNNNGTC 1 cut(s) 408
AjnI CCWGG 1 cut(s) 1176
Alw26I GTCTC 4 cut(s) 481, 1133, 1720, 2834
AlwI GGATC 4 cut(s) 670, 1666, 2119, 2804
AlwNI CAGNNNCTG 3 cut(s) 227, 608, 2021
Ama87I CYCGRG 1 cut(s) 58
Aor13HI TCCGGA 3 cut(s) 412, 1567, 1855
AoxI GGCC 2 cut(s) 763, 3077
ApeKI GCWGC 7 cut(s) 85, 183, 224, 1008, 1234, 2630, 2701
ApoI RAATTY 7 cut(s) 387, 1043, 1313, 1563, 1598, 1707, 2902
ArsI GACNNNNNNTTYG 2 cut(s) 866, 898
AseI ATTAAT 2 cut(s) 177, 651
Asp700I GAANNNNTTC 5 cut(s) 150, 553, 571, 964, 1279
Asp718I GGTACC 1 cut(s) 1780
AspA2I CCTAGG 1 cut(s) 1288
AspLEI GCGC 1 cut(s) 85
AspS9I GGNCC 2 cut(s) 2182, 3010
AsuHPI GGTGA 3 cut(s) 13, 1291, 2973
AvaI CYCGRG 1 cut(s) 58
AvaII GGWCC 2 cut(s) 2182, 3010
AvrII CCTAGG 1 cut(s) 1288
AxyI CCTNAGG 2 cut(s) 275, 609
BaeGI GKGCMC 1 cut(s) 602
BanI GGYRCC 1 cut(s) 1780
BarI GAAGNNNNNNTAC 2 cut(s) 1624, 1656
BauI CACGAG 1 cut(s) 2529
BbsI GAAGAC 4 cut(s) 574, 1658, 2012, 2728
BbvI GCAGC 7 cut(s) 72, 195, 211, 1020, 1221, 2642, 2688
BccI CCATC 7 cut(s) 1381, 1432, 1820, 1889, 2668, 2873, 2908
BcgI CGANNNNNNTGC 4 cut(s) 39, 73, 475, 509
BciT130I CCWGG 1 cut(s) 1178
BciVI GTATCC 1 cut(s) 2685
BclI TGATCA 1 cut(s) 331
BcoDI GTCTC 4 cut(s) 481, 1133, 1720, 2834
BfaI CTAG 6 cut(s) 956, 1289, 1532, 1676, 2156, 2324
BfmI CTRYAG 1 cut(s) 1379
BfrI CTTAAG 1 cut(s) 2951
BfuI GTATCC 1 cut(s) 2685
BglI GCCNNNNNGGC 1 cut(s) 453
BglII AGATCT 1 cut(s) 1678
BisI GCNGC 7 cut(s) 86, 184, 225, 1009, 1235, 2631, 2702
BlnI CCTAGG 1 cut(s) 1288
BlsI GCNGC 7 cut(s) 87, 185, 226, 1010, 1236, 2632, 2703
BmcAI AGTACT 1 cut(s) 426
Bme1390I CCNGG 1 cut(s) 1178
Bme18I GGWCC 2 cut(s) 2182, 3010
BmeRI GACNNNNNGTC 1 cut(s) 408
BmeT110I CYCGRG 1 cut(s) 58
BmgT120I GGNCC 2 cut(s) 2182, 3010
BmiI GGNNCC 2 cut(s) 587, 1782
BmrFI CCNGG 1 cut(s) 1178
BmsI GCATC 5 cut(s) 540, 1011, 1940, 2039, 2355
BoxI GACNNNNGTC 1 cut(s) 3066
BpiI GAAGAC 4 cut(s) 574, 1658, 2012, 2728
BplI GAGNNNNNCTC 4 cut(s) 461, 493, 1984, 2016
BpmI CTGGAG 6 cut(s) 837, 1578, 1790, 2008, 2272, 2619
BpuEI CTTGAG 3 cut(s) 80, 1127, 2999
Bsa29I ATCGAT 1 cut(s) 76
BsaAI YACGTR 1 cut(s) 2926
BsaBI GATNNNNATC 1 cut(s) 1185
BsaJI CCNNGG 5 cut(s) 289, 940, 1176, 1288, 2984
BsaWI WCCGGW 4 cut(s) 412, 656, 1567, 1855
Bsc4I CCNNNNNNNGG 8 cut(s) 257, 276, 545, 595, 596, 761, 1361, 1790
Bse118I RCCGGY 2 cut(s) 452, 1484
Bse1I ACTGG 7 cut(s) 673, 1469, 1524, 1561, 1991, 2015, 2086
Bse21I CCTNAGG 2 cut(s) 275, 609
Bse3DI GCAATG 3 cut(s) 45, 70, 2239
Bse8I GATNNNNATC 1 cut(s) 1185
BseAI TCCGGA 3 cut(s) 412, 1567, 1855
BseBI CCWGG 1 cut(s) 1178
BseCI ATCGAT 1 cut(s) 76
BseDI CCNNGG 5 cut(s) 289, 940, 1176, 1288, 2984
BseJI GATNNNNATC 1 cut(s) 1185
BseLI CCNNNNNNNGG 8 cut(s) 257, 276, 545, 595, 596, 761, 1361, 1790
BseMI GCAATG 3 cut(s) 45, 70, 2239
BseMII CTCAG 9 cut(s) 219, 289, 376, 410, 600, 617, 1128, 1580, 2015
BseNI ACTGG 7 cut(s) 673, 1469, 1524, 1561, 1991, 2015, 2086
BseRI GAGGAG 4 cut(s) 2005, 2372, 2569, 3020
BseSI GKGCMC 1 cut(s) 602
BseXI GCAGC 7 cut(s) 72, 195, 211, 1020, 1221, 2642, 2688
BseYI CCCAGC 1 cut(s) 826
Bsh1236I CGCG 1 cut(s) 83
Bsh1285I CGRYCG 2 cut(s) 42, 2571
BshFI GGCC 2 cut(s) 765, 3079
BshNI GGYRCC 1 cut(s) 1780
BshVI ATCGAT 1 cut(s) 76
BsiEI CGRYCG 2 cut(s) 42, 2571
BsiHKCI CYCGRG 1 cut(s) 58
BsiSI CCGG 7 cut(s) 413, 453, 657, 801, 1485, 1568, 1856
BslFI GGGAC 1 cut(s) 887
BslI CCNNNNNNNGG 8 cut(s) 257, 276, 545, 595, 596, 761, 1361, 1790
BsmAI GTCTC 4 cut(s) 481, 1133, 1720, 2834
BsmFI GGGAC 1 cut(s) 887
BsmI GAATGC 2 cut(s) 1347, 2234
BsnI GGCC 2 cut(s) 765, 3079
BsoBI CYCGRG 1 cut(s) 58
Bsp1286I GDGCHC 1 cut(s) 602
Bsp13I TCCGGA 3 cut(s) 412, 1567, 1855
Bsp143I GATC 7 cut(s) 73, 331, 675, 1671, 1678, 2111, 2809
BspACI CCGC 6 cut(s) 320, 445, 752, 2085, 2118, 2571
BspANI GGCC 2 cut(s) 765, 3079
BspCNI CTCAG 9 cut(s) 220, 288, 375, 411, 601, 618, 1129, 1581, 2014
BspDI ATCGAT 1 cut(s) 76
BspEI TCCGGA 3 cut(s) 412, 1567, 1855
BspFNI CGCG 1 cut(s) 83
BspHI TCATGA 1 cut(s) 2452
BspLI GGNNCC 2 cut(s) 587, 1782
BspMAI CTGCAG 1 cut(s) 1383
BspPI GGATC 4 cut(s) 670, 1666, 2119, 2804
BspT107I GGYRCC 1 cut(s) 1780
BspTI CTTAAG 1 cut(s) 2951
BsrDI GCAATG 3 cut(s) 45, 70, 2239
BsrFI RCCGGY 2 cut(s) 452, 1484
BsrI ACTGG 7 cut(s) 673, 1469, 1524, 1561, 1991, 2015, 2086
BssAI RCCGGY 2 cut(s) 452, 1484
BssECI CCNNGG 5 cut(s) 289, 940, 1176, 1288, 2984
BssMI GATC 7 cut(s) 73, 331, 675, 1671, 1678, 2111, 2809
BssSI CACGAG 1 cut(s) 2529
BssT1I CCWWGG 3 cut(s) 289, 1288, 2984
Bst2BI CACGAG 1 cut(s) 2529
Bst2UI CCWGG 1 cut(s) 1178
Bst4CI ACNGT 7 cut(s) 43, 604, 1308, 2483, 2602, 2750, 2921
Bst6I CTCTTC 2 cut(s) 2098, 2832
BstAFI CTTAAG 1 cut(s) 2951
BstBAI YACGTR 1 cut(s) 2926
BstC8I GCNNGC 1 cut(s) 2968
BstENI CCTNNNNNAGG 2 cut(s) 274, 759
BstFNI CGCG 1 cut(s) 83
BstHHI GCGC 1 cut(s) 85
BstKTI GATC 7 cut(s) 76, 334, 678, 1674, 1681, 2114, 2812
BstMAI GTCTC 4 cut(s) 481, 1133, 1720, 2834
BstMBI GATC 7 cut(s) 73, 331, 675, 1671, 1678, 2111, 2809
BstMCI CGRYCG 2 cut(s) 42, 2571
BstMWI GCNNNNNNNGC 5 cut(s) 453, 1590, 2124, 3047, 3056
BstNI CCWGG 1 cut(s) 1178
BstNSI RCATGY 5 cut(s) 376, 700, 775, 2283, 2970
BstPAI GACNNNNGTC 1 cut(s) 3066
BstSCI CCNGG 1 cut(s) 1176
BstSFI CTRYAG 1 cut(s) 1379
BstSLI GKGCMC 1 cut(s) 602
BstUI CGCG 1 cut(s) 83
BstV1I GCAGC 7 cut(s) 72, 195, 211, 1020, 1221, 2642, 2688
BstV2I GAAGAC 4 cut(s) 574, 1658, 2012, 2728
BstX2I RGATCY 3 cut(s) 1671, 1678, 2809
BstXI CCANNNNNNTGG 1 cut(s) 376
BstYI RGATCY 3 cut(s) 1671, 1678, 2809
Bsu15I ATCGAT 1 cut(s) 76
Bsu36I CCTNAGG 2 cut(s) 275, 609
BsuI GTATCC 1 cut(s) 2685
BsuRI GGCC 2 cut(s) 765, 3079
BsuTUI ATCGAT 1 cut(s) 76
BtsI GCAGTG 1 cut(s) 3045
BtsIMutI CAGTG 6 cut(s) 118, 370, 666, 1816, 2727, 3045
Cac8I GCNNGC 1 cut(s) 2968
CaiI CAGNNNCTG 3 cut(s) 227, 608, 2021
CciI TCATGA 1 cut(s) 2452
CfoI GCGC 1 cut(s) 85
Cfr10I RCCGGY 2 cut(s) 452, 1484
Cfr13I GGNCC 2 cut(s) 2182, 3010
ClaI ATCGAT 1 cut(s) 76
Csp6I GTAC 4 cut(s) 425, 793, 1527, 1781
CspCI CAANNNNNGTGG 6 cut(s) 108, 143, 525, 560, 889, 924
CviQI GTAC 4 cut(s) 425, 793, 1527, 1781
DpnI GATC 7 cut(s) 75, 333, 677, 1673, 1680, 2113, 2811
DpnII GATC 7 cut(s) 73, 331, 675, 1671, 1678, 2111, 2809
DraI TTTAAA 1 cut(s) 2907
DraIII CACNNNGTG 1 cut(s) 3031
DriI GACNNNNNGTC 1 cut(s) 408
Eam1104I CTCTTC 2 cut(s) 2098, 2832
Eam1105I GACNNNNNGTC 1 cut(s) 408
EarI CTCTTC 2 cut(s) 2098, 2832
EciI GGCGGA 2 cut(s) 741, 2100
Eco130I CCWWGG 3 cut(s) 289, 1288, 2984
Eco147I AGGCCT 1 cut(s) 765
Eco32I GATATC 2 cut(s) 868, 2869
Eco47I GGWCC 2 cut(s) 2182, 3010
Eco57I CTGAAG 5 cut(s) 450, 1037, 1652, 1943, 2043
Eco81I CCTNAGG 2 cut(s) 275, 609
Eco88I CYCGRG 1 cut(s) 58
EcoNI CCTNNNNNAGG 2 cut(s) 274, 759
EcoO109I RGGNCCY 2 cut(s) 2182, 3010
EcoRI GAATTC 3 cut(s) 387, 1313, 1563
EcoRII CCWGG 1 cut(s) 1176
EcoRV GATATC 2 cut(s) 868, 2869
EcoT14I CCWWGG 3 cut(s) 289, 1288, 2984
EcoT22I ATGCAT 1 cut(s) 2370
ErhI CCWWGG 3 cut(s) 289, 1288, 2984
FalI AAGNNNNNCTT 4 cut(s) 1605, 1637, 2778, 2810
FaqI GGGAC 1 cut(s) 887
FauI CCCGC 1 cut(s) 2111
FbaI TGATCA 1 cut(s) 331
FblI GTMKAC 2 cut(s) 2133, 2745
Fnu4HI GCNGC 7 cut(s) 86, 184, 225, 1009, 1235, 2631, 2702
Fsp4HI GCNGC 7 cut(s) 86, 184, 225, 1009, 1235, 2631, 2702
FspBI CTAG 6 cut(s) 956, 1289, 1532, 1676, 2156, 2324
GlaI GCGC 1 cut(s) 84
GluI GCNGC 7 cut(s) 86, 184, 225, 1009, 1235, 2631, 2702
GsaI CCCAGC 1 cut(s) 830
GsuI CTGGAG 6 cut(s) 837, 1578, 1790, 2008, 2272, 2619
HaeIII GGCC 2 cut(s) 765, 3079
HapII CCGG 7 cut(s) 413, 453, 657, 801, 1485, 1568, 1856
HhaI GCGC 1 cut(s) 85
Hin6I GCGC 1 cut(s) 83
HinP1I GCGC 1 cut(s) 83
HincII GTYRAC 3 cut(s) 483, 508, 1039
HindII GTYRAC 3 cut(s) 483, 508, 1039
HindIII AAGCTT 2 cut(s) 488, 1406
HpaII CCGG 7 cut(s) 413, 453, 657, 801, 1485, 1568, 1856
HphI GGTGA 3 cut(s) 13, 1291, 2973
Hpy99I CGWCG 1 cut(s) 3072
HpyCH4III ACNGT 7 cut(s) 43, 604, 1308, 2483, 2602, 2750, 2921
HpyCH4IV ACGT 5 cut(s) 504, 725, 1320, 2502, 2925
HpyF10VI GCNNNNNNNGC 5 cut(s) 453, 1590, 2124, 3047, 3056
HpySE526I ACGT 5 cut(s) 504, 725, 1320, 2502, 2925
HspAI GCGC 1 cut(s) 83
Kpn2I TCCGGA 3 cut(s) 412, 1567, 1855
KpnI GGTACC 1 cut(s) 1784
Ksp22I TGATCA 1 cut(s) 331
Kzo9I GATC 7 cut(s) 73, 331, 675, 1671, 1678, 2111, 2809
LmnI GCTCC 4 cut(s) 1958, 2291, 2638, 3019
Lsp1109I GCAGC 7 cut(s) 72, 195, 211, 1020, 1221, 2642, 2688
LweI GCATC 5 cut(s) 540, 1011, 1940, 2039, 2355
MaeI CTAG 6 cut(s) 956, 1289, 1532, 1676, 2156, 2324
MaeII ACGT 5 cut(s) 504, 725, 1320, 2502, 2925
MaeIII GTNAC 9 cut(s) 113, 202, 296, 1224, 1364, 1467, 2483, 2684, 2921
MalI GATC 7 cut(s) 75, 333, 677, 1673, 1680, 2113, 2811
MboI GATC 7 cut(s) 73, 331, 675, 1671, 1678, 2111, 2809
MfeI CAATTG 3 cut(s) 1003, 2619, 2705
MflI RGATCY 3 cut(s) 1671, 1678, 2809
MhlI GDGCHC 1 cut(s) 602
MlyI GAGTC 1 cut(s) 2570
MmeI TCCRAC 3 cut(s) 357, 367, 2969
Mph1103I ATGCAT 1 cut(s) 2370
MroI TCCGGA 3 cut(s) 412, 1567, 1855
MroXI GAANNNNTTC 5 cut(s) 150, 553, 571, 964, 1279
MslI CAYNNNNRTG 1 cut(s) 671
MspA1I CMGCKG 1 cut(s) 224
MspCI CTTAAG 1 cut(s) 2951
MspI CCGG 7 cut(s) 413, 453, 657, 801, 1485, 1568, 1856
MspR9I CCNGG 1 cut(s) 1178
MunI CAATTG 3 cut(s) 1003, 2619, 2705
Mva1269I GAATGC 2 cut(s) 1347, 2234
MvaI CCWGG 1 cut(s) 1178
MvnI CGCG 1 cut(s) 83
MwoI GCNNNNNNNGC 5 cut(s) 453, 1590, 2124, 3047, 3056
NdeII GATC 7 cut(s) 73, 331, 675, 1671, 1678, 2111, 2809
NlaIV GGNNCC 2 cut(s) 587, 1782
NmuCI GTSAC 3 cut(s) 113, 1467, 2483
NsiI ATGCAT 1 cut(s) 2370
NspI RCATGY 5 cut(s) 376, 700, 775, 2283, 2970
PaeI GCATGC 1 cut(s) 2970
PaeR7I CTCGAG 1 cut(s) 58
PagI TCATGA 1 cut(s) 2452
PceI AGGCCT 1 cut(s) 765
PciI ACATGT 3 cut(s) 372, 696, 2279
PctI GAATGC 2 cut(s) 1347, 2234
PdmI GAANNNNTTC 5 cut(s) 150, 553, 571, 964, 1279
PflMI CCANNNNNTGG 2 cut(s) 257, 1361
PkrI GCNGC 7 cut(s) 87, 185, 226, 1010, 1236, 2632, 2703
PleI GAGTC 1 cut(s) 2570
PpsI GAGTC 1 cut(s) 2570
Ppu21I YACGTR 1 cut(s) 2926
PpuMI RGGWCCY 2 cut(s) 2182, 3010
PscI ACATGT 3 cut(s) 372, 696, 2279
PshAI GACNNNNGTC 1 cut(s) 3066
PshBI ATTAAT 2 cut(s) 177, 651
PsiI TTATAA 1 cut(s) 1545
Psp1406I AACGTT 2 cut(s) 504, 2502
Psp5II RGGWCCY 2 cut(s) 2182, 3010
Psp6I CCWGG 1 cut(s) 1176
PspFI CCCAGC 1 cut(s) 826
PspGI CCWGG 1 cut(s) 1176
PspN4I GGNNCC 2 cut(s) 587, 1782
PspPI GGNCC 2 cut(s) 2182, 3010
PspPPI RGGWCCY 2 cut(s) 2182, 3010
PspXI VCTCGAGB 1 cut(s) 58
PsrI GAACNNNNNNTAC 6 cut(s) 408, 440, 879, 911, 1851, 1883
PstI CTGCAG 1 cut(s) 1383
PstNI CAGNNNCTG 3 cut(s) 227, 608, 2021
PsuI RGATCY 3 cut(s) 1671, 1678, 2809
PvuII CAGCTG 1 cut(s) 224
RsaI GTAC 4 cut(s) 426, 794, 1528, 1782
RsaNI GTAC 4 cut(s) 425, 793, 1527, 1781
RseI CAYNNNNRTG 1 cut(s) 671
SatI GCNGC 7 cut(s) 86, 184, 225, 1009, 1235, 2631, 2702
Sau3AI GATC 7 cut(s) 73, 331, 675, 1671, 1678, 2111, 2809
Sau96I GGNCC 2 cut(s) 2182, 3010
ScaI AGTACT 1 cut(s) 426
SchI GAGTC 1 cut(s) 2570
ScrFI CCNGG 1 cut(s) 1178
SduI GDGCHC 1 cut(s) 602
SfaNI GCATC 5 cut(s) 540, 1011, 1940, 2039, 2355
SfcI CTRYAG 1 cut(s) 1379
Sfr274I CTCGAG 1 cut(s) 58
SinI GGWCC 2 cut(s) 2182, 3010
SlaI CTCGAG 1 cut(s) 58
SmiMI CAYNNNNRTG 1 cut(s) 671
SmlI CTYRAG 5 cut(s) 58, 95, 1106, 2951, 3014
SmoI CTYRAG 5 cut(s) 58, 95, 1106, 2951, 3014
SphI GCATGC 1 cut(s) 2970
SseBI AGGCCT 1 cut(s) 765
SsiI CCGC 6 cut(s) 320, 445, 752, 2085, 2118, 2571
SspI AATATT 2 cut(s) 1360, 2416
SspMI CTAG 6 cut(s) 956, 1289, 1532, 1676, 2156, 2324
StuI AGGCCT 1 cut(s) 765
StyD4I CCNGG 1 cut(s) 1176
StyI CCWWGG 3 cut(s) 289, 1288, 2984
TaaI ACNGT 7 cut(s) 43, 604, 1308, 2483, 2602, 2750, 2921
TaiI ACGT 5 cut(s) 507, 728, 1323, 2505, 2928
TaqI TCGA 6 cut(s) 38, 59, 76, 2096, 2871, 3070
TatI WGTACW 2 cut(s) 424, 1526
TscAI CASTG 6 cut(s) 118, 370, 673, 1816, 2734, 3052
TseFI GTSAC 3 cut(s) 113, 1467, 2483
TseI GCWGC 7 cut(s) 85, 183, 224, 1008, 1234, 2630, 2701
Tsp45I GTSAC 3 cut(s) 113, 1467, 2483
TspGWI ACGGA 2 cut(s) 1945, 3056
TspRI CASTG 6 cut(s) 118, 370, 673, 1816, 2734, 3052
Van91I CCANNNNNTGG 2 cut(s) 257, 1361
Vha464I CTTAAG 1 cut(s) 2951
VpaK11BI GGWCC 2 cut(s) 2182, 3010
VspI ATTAAT 2 cut(s) 177, 651
XagI CCTNNNNNAGG 2 cut(s) 274, 759
XapI RAATTY 7 cut(s) 387, 1043, 1313, 1563, 1598, 1707, 2902
XceI RCATGY 5 cut(s) 376, 700, 775, 2283, 2970
XcmI CCANNNNNNNNNTGG 1 cut(s) 2218
XhoI CTCGAG 1 cut(s) 58
XmaJI CCTAGG 1 cut(s) 1288
XmiI GTMKAC 2 cut(s) 2133, 2745
XmnI GAANNNNTTC 5 cut(s) 150, 553, 571, 964, 1279
XspI CTAG 6 cut(s) 956, 1289, 1532, 1676, 2156, 2324
ZrmI AGTACT 1 cut(s) 426
Zsp2I ATGCAT 1 cut(s) 2370
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.