Belongs to the protein kinase superfamily. Ser Thr protein kinase family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 641 |
| AciI | CCGC | 1 cut(s) | 442 |
| AclWI | GGATC | 2 cut(s) | 455, 468 |
| AcsI | RAATTY | 1 cut(s) | 131 |
| AfaI | GTAC | 2 cut(s) | 268, 514 |
| AflIII | ACRYGT | 1 cut(s) | 601 |
| AgsI | TTSAA | 3 cut(s) | 28, 178, 340 |
| AjnI | CCWGG | 2 cut(s) | 307, 416 |
| AluBI | AGCT | 4 cut(s) | 11, 394, 454, 610 |
| AluI | AGCT | 4 cut(s) | 11, 394, 454, 610 |
| Alw21I | GWGCWC | 1 cut(s) | 648 |
| Alw26I | GTCTC | 3 cut(s) | 156, 308, 434 |
| AlwI | GGATC | 2 cut(s) | 455, 468 |
| AoxI | GGCC | 2 cut(s) | 55, 479 |
| ApoI | RAATTY | 1 cut(s) | 131 |
| AspS9I | GGNCC | 3 cut(s) | 32, 56, 653 |
| AvaII | GGWCC | 2 cut(s) | 32, 653 |
| BamHI | GGATCC | 1 cut(s) | 460 |
| Bbv12I | GWGCWC | 1 cut(s) | 648 |
| BccI | CCATC | 2 cut(s) | 179, 500 |
| BciT130I | CCWGG | 2 cut(s) | 309, 418 |
| BcoDI | GTCTC | 3 cut(s) | 156, 308, 434 |
| BfmI | CTRYAG | 1 cut(s) | 447 |
| BfuAI | ACCTGC | 1 cut(s) | 641 |
| BmcAI | AGTACT | 1 cut(s) | 268 |
| Bme1390I | CCNGG | 2 cut(s) | 309, 418 |
| Bme18I | GGWCC | 2 cut(s) | 32, 653 |
| BmgT120I | GGNCC | 3 cut(s) | 32, 56, 653 |
| BmiI | GGNNCC | 1 cut(s) | 462 |
| BmrFI | CCNGG | 2 cut(s) | 309, 418 |
| BmsI | GCATC | 1 cut(s) | 358 |
| Bpu10I | CCTNAGC | 1 cut(s) | 423 |
| BsaI | GGTCTC | 1 cut(s) | 434 |
| BsaJI | CCNNGG | 3 cut(s) | 308, 417, 498 |
| Bse1I | ACTGG | 2 cut(s) | 67, 274 |
| BseBI | CCWGG | 2 cut(s) | 309, 418 |
| BseDI | CCNNGG | 3 cut(s) | 308, 417, 498 |
| BseGI | GGATG | 1 cut(s) | 638 |
| BseMII | CTCAG | 1 cut(s) | 337 |
| BseNI | ACTGG | 2 cut(s) | 67, 274 |
| BseYI | CCCAGC | 1 cut(s) | 72 |
| BshFI | GGCC | 2 cut(s) | 57, 481 |
| BsiHKAI | GWGCWC | 1 cut(s) | 648 |
| BslFI | GGGAC | 2 cut(s) | 89, 612 |
| BsmAI | GTCTC | 3 cut(s) | 156, 308, 434 |
| BsmFI | GGGAC | 2 cut(s) | 89, 612 |
| BsnI | GGCC | 2 cut(s) | 57, 481 |
| Bso31I | GGTCTC | 1 cut(s) | 434 |
| Bsp1286I | GDGCHC | 1 cut(s) | 648 |
| Bsp143I | GATC | 3 cut(s) | 103, 460, 473 |
| Bsp19I | CCATGG | 1 cut(s) | 498 |
| BspACI | CCGC | 1 cut(s) | 442 |
| BspANI | GGCC | 2 cut(s) | 57, 481 |
| BspCNI | CTCAG | 1 cut(s) | 336 |
| BspHI | TCATGA | 1 cut(s) | 432 |
| BspLI | GGNNCC | 1 cut(s) | 462 |
| BspMI | ACCTGC | 1 cut(s) | 641 |
| BspPI | GGATC | 2 cut(s) | 455, 468 |
| BspTNI | GGTCTC | 1 cut(s) | 434 |
| BsrI | ACTGG | 2 cut(s) | 67, 274 |
| BssECI | CCNNGG | 3 cut(s) | 308, 417, 498 |
| BssMI | GATC | 3 cut(s) | 103, 460, 473 |
| BssT1I | CCWWGG | 1 cut(s) | 498 |
| Bst2UI | CCWGG | 2 cut(s) | 309, 418 |
| Bst4CI | ACNGT | 3 cut(s) | 387, 490, 577 |
| Bst6I | CTCTTC | 1 cut(s) | 84 |
| BstC8I | GCNNGC | 2 cut(s) | 440, 648 |
| BstDEI | CTNAG | 2 cut(s) | 323, 423 |
| BstDSI | CCRYGG | 1 cut(s) | 498 |
| BstF5I | GGATG | 1 cut(s) | 638 |
| BstKTI | GATC | 3 cut(s) | 106, 463, 476 |
| BstMAI | GTCTC | 3 cut(s) | 156, 308, 434 |
| BstMBI | GATC | 3 cut(s) | 103, 460, 473 |
| BstNI | CCWGG | 2 cut(s) | 309, 418 |
| BstNSI | RCATGY | 2 cut(s) | 231, 605 |
| BstSCI | CCNGG | 2 cut(s) | 307, 416 |
| BstSFI | CTRYAG | 1 cut(s) | 447 |
| BstX2I | RGATCY | 1 cut(s) | 460 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 29 |
| BstYI | RGATCY | 1 cut(s) | 460 |
| BsuRI | GGCC | 2 cut(s) | 57, 481 |
| BtgI | CCRYGG | 1 cut(s) | 498 |
| BtsCI | GGATG | 1 cut(s) | 638 |
| BtsIMutI | CAGTG | 2 cut(s) | 208, 495 |
| BveI | ACCTGC | 1 cut(s) | 641 |
| Cac8I | GCNNGC | 2 cut(s) | 440, 648 |
| CciI | TCATGA | 1 cut(s) | 432 |
| Cfr13I | GGNCC | 3 cut(s) | 32, 56, 653 |
| Csp6I | GTAC | 2 cut(s) | 267, 513 |
| CviAII | CATG | 7 cut(s) | 228, 241, 304, 433, 477, 499, 602 |
| CviQI | GTAC | 2 cut(s) | 267, 513 |
| DdeI | CTNAG | 2 cut(s) | 323, 423 |
| DpnI | GATC | 3 cut(s) | 105, 462, 475 |
| DpnII | GATC | 3 cut(s) | 103, 460, 473 |
| Eam1104I | CTCTTC | 1 cut(s) | 84 |
| EarI | CTCTTC | 1 cut(s) | 84 |
| Eco130I | CCWWGG | 1 cut(s) | 498 |
| Eco31I | GGTCTC | 1 cut(s) | 434 |
| Eco47I | GGWCC | 2 cut(s) | 32, 653 |
| EcoO109I | RGGNCCY | 2 cut(s) | 56, 653 |
| EcoRII | CCWGG | 2 cut(s) | 307, 416 |
| EcoT14I | CCWWGG | 1 cut(s) | 498 |
| ErhI | CCWWGG | 1 cut(s) | 498 |
| FaeI | CATG | 7 cut(s) | 231, 244, 307, 436, 480, 502, 605 |
| FaqI | GGGAC | 2 cut(s) | 89, 612 |
| FatI | CATG | 7 cut(s) | 227, 240, 303, 432, 476, 498, 601 |
| FokI | GGATG | 1 cut(s) | 645 |
| GsaI | CCCAGC | 1 cut(s) | 76 |
| HaeIII | GGCC | 2 cut(s) | 57, 481 |
| Hin1II | CATG | 7 cut(s) | 231, 244, 307, 436, 480, 502, 605 |
| HincII | GTYRAC | 1 cut(s) | 19 |
| HindII | GTYRAC | 1 cut(s) | 19 |
| HindIII | AAGCTT | 1 cut(s) | 608 |
| HinfI | GANTC | 2 cut(s) | 321, 327 |
| Hpy166II | GTNNAC | 2 cut(s) | 19, 515 |
| Hpy188I | TCNGA | 2 cut(s) | 326, 346 |
| Hpy188III | TCNNGA | 2 cut(s) | 433, 458 |
| Hpy8I | GTNNAC | 2 cut(s) | 19, 515 |
| HpyAV | CCTTC | 3 cut(s) | 133, 152, 589 |
| HpyCH4III | ACNGT | 3 cut(s) | 387, 490, 577 |
| HpyCH4IV | ACGT | 1 cut(s) | 78 |
| HpyCH4V | TGCA | 3 cut(s) | 45, 227, 546 |
| HpyF3I | CTNAG | 2 cut(s) | 323, 423 |
| HpySE526I | ACGT | 1 cut(s) | 78 |
| Hsp92II | CATG | 7 cut(s) | 231, 244, 307, 436, 480, 502, 605 |
| Kzo9I | GATC | 3 cut(s) | 103, 460, 473 |
| LweI | GCATC | 1 cut(s) | 358 |
| MaeII | ACGT | 1 cut(s) | 78 |
| MaeIII | GTNAC | 3 cut(s) | 205, 484, 490 |
| MalI | GATC | 3 cut(s) | 105, 462, 475 |
| MboI | GATC | 3 cut(s) | 103, 460, 473 |
| MboII | GAAGA | 5 cut(s) | 101, 103, 139, 257, 515 |
| MflI | RGATCY | 1 cut(s) | 460 |
| MhlI | GDGCHC | 1 cut(s) | 648 |
| MluCI | AATT | 4 cut(s) | 83, 131, 178, 190 |
| MlyI | GAGTC | 1 cut(s) | 315 |
| MnlI | CCTC | 5 cut(s) | 47, 148, 285, 367, 622 |
| MseI | TTAA | 2 cut(s) | 360, 561 |
| MslI | CAYNNNNRTG | 1 cut(s) | 27 |
| MspA1I | CMGCKG | 1 cut(s) | 394 |
| MspR9I | CCNGG | 2 cut(s) | 309, 418 |
| MvaI | CCWGG | 2 cut(s) | 309, 418 |
| NcoI | CCATGG | 1 cut(s) | 498 |
| NdeII | GATC | 3 cut(s) | 103, 460, 473 |
| NlaIII | CATG | 7 cut(s) | 231, 244, 307, 436, 480, 502, 605 |
| NlaIV | GGNNCC | 1 cut(s) | 462 |
| NmuCI | GTSAC | 2 cut(s) | 205, 490 |
| NspI | RCATGY | 2 cut(s) | 231, 605 |
| PagI | TCATGA | 1 cut(s) | 432 |
| PciI | ACATGT | 1 cut(s) | 601 |
| PfeI | GAWTC | 1 cut(s) | 327 |
| PleI | GAGTC | 1 cut(s) | 315 |
| PpsI | GAGTC | 1 cut(s) | 315 |
| PpuMI | RGGWCCY | 1 cut(s) | 653 |
| PscI | ACATGT | 1 cut(s) | 601 |
| Psp5II | RGGWCCY | 1 cut(s) | 653 |
| Psp6I | CCWGG | 2 cut(s) | 307, 416 |
| PspFI | CCCAGC | 1 cut(s) | 72 |
| PspGI | CCWGG | 2 cut(s) | 307, 416 |
| PspN4I | GGNNCC | 1 cut(s) | 462 |
| PspPI | GGNCC | 3 cut(s) | 32, 56, 653 |
| PspPPI | RGGWCCY | 1 cut(s) | 653 |
| PsuI | RGATCY | 1 cut(s) | 460 |
| PvuII | CAGCTG | 1 cut(s) | 394 |
| RsaI | GTAC | 2 cut(s) | 268, 514 |
| RsaNI | GTAC | 2 cut(s) | 267, 513 |
| RseI | CAYNNNNRTG | 1 cut(s) | 27 |
| SaqAI | TTAA | 2 cut(s) | 360, 561 |
| Sau3AI | GATC | 3 cut(s) | 103, 460, 473 |
| Sau96I | GGNCC | 3 cut(s) | 32, 56, 653 |
| ScaI | AGTACT | 1 cut(s) | 268 |
| SchI | GAGTC | 1 cut(s) | 315 |
| ScrFI | CCNGG | 2 cut(s) | 309, 418 |
| SduI | GDGCHC | 1 cut(s) | 648 |
| SetI | ASST | 6 cut(s) | 13, 81, 396, 456, 612, 655 |
| SfaNI | GCATC | 1 cut(s) | 358 |
| SfcI | CTRYAG | 1 cut(s) | 447 |
| SinI | GGWCC | 2 cut(s) | 32, 653 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 27 |
| Sse9I | AATT | 4 cut(s) | 83, 131, 178, 190 |
| SsiI | CCGC | 1 cut(s) | 442 |
| StyD4I | CCNGG | 2 cut(s) | 307, 416 |
| StyI | CCWWGG | 1 cut(s) | 498 |
| TaaI | ACNGT | 3 cut(s) | 387, 490, 577 |
| TaiI | ACGT | 1 cut(s) | 81 |
| TaqI | TCGA | 1 cut(s) | 52 |
| TasI | AATT | 4 cut(s) | 83, 131, 178, 190 |
| TatI | WGTACW | 1 cut(s) | 266 |
| TfiI | GAWTC | 1 cut(s) | 327 |
| Tru1I | TTAA | 2 cut(s) | 360, 561 |
| Tru9I | TTAA | 2 cut(s) | 360, 561 |
| TscAI | CASTG | 2 cut(s) | 208, 495 |
| TseFI | GTSAC | 2 cut(s) | 205, 490 |
| Tsp45I | GTSAC | 2 cut(s) | 205, 490 |
| TspDTI | ATGAA | 3 cut(s) | 183, 234, 536 |
| TspRI | CASTG | 2 cut(s) | 208, 495 |
| VpaK11BI | GGWCC | 2 cut(s) | 32, 653 |
| XapI | RAATTY | 1 cut(s) | 131 |
| XceI | RCATGY | 2 cut(s) | 231, 605 |
| ZrmI | AGTACT | 1 cut(s) | 268 |