Belongs to the protein kinase superfamily. Ser Thr protein kinase family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 1062 |
| AarI | CACCTGC | 1 cut(s) | 2803 |
| AasI | GACNNNNNNGTC | 2 cut(s) | 375, 381 |
| AatII | GACGTC | 1 cut(s) | 379 |
| Acc16I | TGCGCA | 1 cut(s) | 436 |
| Acc36I | ACCTGC | 1 cut(s) | 2803 |
| Acc65I | GGTACC | 1 cut(s) | 1807 |
| AccB1I | GGYRCC | 2 cut(s) | 623, 1807 |
| AccB7I | CCANNNNNTGG | 3 cut(s) | 281, 1385, 1402 |
| AccI | GTMKAC | 1 cut(s) | 2160 |
| AciI | CCGC | 5 cut(s) | 344, 469, 776, 924, 2042 |
| AclWI | GGATC | 6 cut(s) | 134, 694, 1294, 1307, 1762, 2753 |
| AcsI | RAATTY | 8 cut(s) | 116, 411, 483, 937, 1276, 1590, 1625, 2878 |
| AcuI | CTGAAG | 4 cut(s) | 292, 474, 1562, 1679 |
| AcyI | GRCGYC | 1 cut(s) | 376 |
| AfaI | GTAC | 5 cut(s) | 239, 391, 1516, 1809, 1917 |
| AfiI | CCNNNNNNNGG | 7 cut(s) | 281, 300, 569, 785, 1385, 1402, 2077 |
| AflII | CTTAAG | 1 cut(s) | 2927 |
| AflIII | ACRYGT | 2 cut(s) | 396, 2306 |
| AjnI | CCWGG | 4 cut(s) | 778, 1200, 2662, 2734 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 1113, 1145, 1937, 1969 |
| AleI | CACNNNNGTG | 1 cut(s) | 2900 |
| Alw21I | GWGCWC | 1 cut(s) | 2897 |
| Alw26I | GTCTC | 5 cut(s) | 383, 389, 683, 1747, 2780 |
| AlwI | GGATC | 6 cut(s) | 134, 694, 1294, 1307, 1762, 2753 |
| AoxI | GGCC | 3 cut(s) | 643, 787, 1462 |
| ApeKI | GCWGC | 6 cut(s) | 955, 1032, 1679, 1891, 2392, 2657 |
| ApoI | RAATTY | 8 cut(s) | 116, 411, 483, 937, 1276, 1590, 1625, 2878 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 2337, 2369 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 988, 2070 |
| Asp718I | GGTACC | 1 cut(s) | 1807 |
| AspLEI | GCGC | 1 cut(s) | 437 |
| AspS9I | GGNCC | 2 cut(s) | 2209, 2986 |
| AsuHPI | GGTGA | 6 cut(s) | 264, 1315, 1420, 1814, 2576, 2949 |
| AsuII | TTCGAA | 1 cut(s) | 1828 |
| AvaII | GGWCC | 2 cut(s) | 2209, 2986 |
| BaeGI | GKGCMC | 2 cut(s) | 626, 900 |
| BamHI | GGATCC | 1 cut(s) | 1299 |
| BanI | GGYRCC | 2 cut(s) | 623, 1807 |
| BbsI | GAAGAC | 3 cut(s) | 1685, 2677, 2868 |
| Bbv12I | GWGCWC | 1 cut(s) | 2897 |
| BbvI | GCAGC | 6 cut(s) | 967, 1044, 1666, 1903, 2379, 2644 |
| BccI | CCATC | 6 cut(s) | 563, 600, 1312, 1405, 1456, 1847 |
| BceAI | ACGGC | 1 cut(s) | 751 |
| BciT130I | CCWGG | 4 cut(s) | 780, 1202, 2664, 2736 |
| BclI | TGATCA | 1 cut(s) | 2176 |
| BcoDI | GTCTC | 5 cut(s) | 383, 389, 683, 1747, 2780 |
| BfaI | CTAG | 7 cut(s) | 530, 641, 980, 1703, 1796, 2316, 2351 |
| BfmI | CTRYAG | 3 cut(s) | 1092, 1748, 2049 |
| BfrI | CTTAAG | 1 cut(s) | 2927 |
| BfuAI | ACCTGC | 1 cut(s) | 2803 |
| BglII | AGATCT | 2 cut(s) | 1705, 2298 |
| BisI | GCNGC | 6 cut(s) | 956, 1033, 1680, 1892, 2393, 2658 |
| BlsI | GCNGC | 6 cut(s) | 957, 1034, 1681, 1893, 2394, 2659 |
| Bme1390I | CCNGG | 4 cut(s) | 780, 1202, 2664, 2736 |
| Bme18I | GGWCC | 2 cut(s) | 2209, 2986 |
| BmgT120I | GGNCC | 2 cut(s) | 2209, 2986 |
| BmiI | GGNNCC | 9 cut(s) | 265, 553, 625, 1301, 1646, 1809, 1942, 2288, 2644 |
| BmrFI | CCNGG | 4 cut(s) | 780, 1202, 2664, 2736 |
| BmsI | GCATC | 4 cut(s) | 529, 747, 2736, 3004 |
| BpiI | GAAGAC | 3 cut(s) | 1685, 2677, 2868 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 2011, 2043 |
| BpmI | CTGGAG | 5 cut(s) | 791, 935, 1061, 1424, 2646 |
| Bpu10I | CCTNAGC | 1 cut(s) | 2140 |
| Bpu14I | TTCGAA | 1 cut(s) | 1828 |
| BpuEI | CTTGAG | 1 cut(s) | 1216 |
| BsaBI | GATNNNNATC | 1 cut(s) | 1012 |
| BsaHI | GRCGYC | 1 cut(s) | 376 |
| BsaI | GGTCTC | 1 cut(s) | 683 |
| BsaJI | CCNNGG | 6 cut(s) | 567, 1129, 1639, 1674, 1965, 2960 |
| BsaWI | WCCGGW | 1 cut(s) | 1583 |
| Bsc4I | CCNNNNNNNGG | 7 cut(s) | 281, 300, 569, 785, 1385, 1402, 2077 |
| Bse118I | RCCGGY | 1 cut(s) | 476 |
| Bse1I | ACTGG | 1 cut(s) | 595 |
| Bse3DI | GCAATG | 3 cut(s) | 94, 2266, 2533 |
| Bse8I | GATNNNNATC | 1 cut(s) | 1012 |
| BseBI | CCWGG | 4 cut(s) | 780, 1202, 2664, 2736 |
| BseDI | CCNNGG | 6 cut(s) | 567, 1129, 1639, 1674, 1965, 2960 |
| BseGI | GGATG | 4 cut(s) | 555, 611, 1858, 1982 |
| BseJI | GATNNNNATC | 1 cut(s) | 1012 |
| BseLI | CCNNNNNNNGG | 7 cut(s) | 281, 300, 569, 785, 1385, 1402, 2077 |
| BseMI | GCAATG | 3 cut(s) | 94, 2266, 2533 |
| BseMII | CTCAG | 7 cut(s) | 313, 394, 400, 434, 887, 1046, 1607 |
| BseNI | ACTGG | 1 cut(s) | 595 |
| BseRI | GAGGAG | 2 cut(s) | 2032, 2399 |
| BseSI | GKGCMC | 2 cut(s) | 626, 900 |
| BseXI | GCAGC | 6 cut(s) | 967, 1044, 1666, 1903, 2379, 2644 |
| BseYI | CCCAGC | 1 cut(s) | 1907 |
| Bsh1285I | CGRYCG | 1 cut(s) | 100 |
| BshFI | GGCC | 3 cut(s) | 645, 789, 1464 |
| BshNI | GGYRCC | 2 cut(s) | 623, 1807 |
| BsiEI | CGRYCG | 1 cut(s) | 100 |
| BsiHKAI | GWGCWC | 1 cut(s) | 2897 |
| BsiSI | CCGG | 2 cut(s) | 477, 1584 |
| BslFI | GGGAC | 1 cut(s) | 317 |
| BslI | CCNNNNNNNGG | 7 cut(s) | 281, 300, 569, 785, 1385, 1402, 2077 |
| BsmAI | GTCTC | 5 cut(s) | 383, 389, 683, 1747, 2780 |
| BsmBI | CGTCTC | 1 cut(s) | 383 |
| BsmFI | GGGAC | 1 cut(s) | 317 |
| BsmI | GAATGC | 2 cut(s) | 1371, 2261 |
| BsnI | GGCC | 3 cut(s) | 645, 789, 1464 |
| Bso31I | GGTCTC | 1 cut(s) | 683 |
| Bsp119I | TTCGAA | 1 cut(s) | 1828 |
| Bsp1286I | GDGCHC | 3 cut(s) | 626, 900, 2897 |
| BspACI | CCGC | 5 cut(s) | 344, 469, 776, 924, 2042 |
| BspANI | GGCC | 3 cut(s) | 645, 789, 1464 |
| BspCNI | CTCAG | 7 cut(s) | 312, 393, 399, 435, 886, 1047, 1608 |
| BspHI | TCATGA | 1 cut(s) | 2479 |
| BspLI | GGNNCC | 9 cut(s) | 265, 553, 625, 1301, 1646, 1809, 1942, 2288, 2644 |
| BspMAI | CTGCAG | 1 cut(s) | 2053 |
| BspMI | ACCTGC | 1 cut(s) | 2803 |
| BspPI | GGATC | 6 cut(s) | 134, 694, 1294, 1307, 1762, 2753 |
| BspT104I | TTCGAA | 1 cut(s) | 1828 |
| BspT107I | GGYRCC | 2 cut(s) | 623, 1807 |
| BspTI | CTTAAG | 1 cut(s) | 2927 |
| BspTNI | GGTCTC | 1 cut(s) | 683 |
| BsrDI | GCAATG | 3 cut(s) | 94, 2266, 2533 |
| BsrFI | RCCGGY | 1 cut(s) | 476 |
| BsrI | ACTGG | 1 cut(s) | 595 |
| BssAI | RCCGGY | 1 cut(s) | 476 |
| BssECI | CCNNGG | 6 cut(s) | 567, 1129, 1639, 1674, 1965, 2960 |
| BssNAI | GTATAC | 1 cut(s) | 2161 |
| BssNI | GRCGYC | 1 cut(s) | 376 |
| BssT1I | CCWWGG | 6 cut(s) | 567, 1129, 1639, 1674, 1965, 2960 |
| Bst1107I | GTATAC | 1 cut(s) | 2161 |
| Bst2UI | CCWGG | 4 cut(s) | 780, 1202, 2664, 2736 |
| Bst4CI | ACNGT | 3 cut(s) | 70, 1184, 2629 |
| Bst6I | CTCTTC | 5 cut(s) | 1989, 2734, 2772, 2778, 2976 |
| BstACI | GRCGYC | 1 cut(s) | 376 |
| BstAFI | CTTAAG | 1 cut(s) | 2927 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1258 |
| BstBI | TTCGAA | 1 cut(s) | 1828 |
| BstC8I | GCNNGC | 2 cut(s) | 1903, 2944 |
| BstENI | CCTNNNNNAGG | 3 cut(s) | 298, 783, 2075 |
| BstF5I | GGATG | 4 cut(s) | 555, 611, 1858, 1982 |
| BstHHI | GCGC | 1 cut(s) | 437 |
| BstMAI | GTCTC | 5 cut(s) | 383, 389, 683, 1747, 2780 |
| BstMCI | CGRYCG | 1 cut(s) | 100 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 477, 883, 1258, 1617 |
| BstNI | CCWGG | 4 cut(s) | 780, 1202, 2664, 2736 |
| BstNSI | RCATGY | 5 cut(s) | 400, 799, 1905, 2310, 2946 |
| BstSCI | CCNGG | 4 cut(s) | 778, 1200, 2662, 2734 |
| BstSFI | CTRYAG | 3 cut(s) | 1092, 1748, 2049 |
| BstSLI | GKGCMC | 2 cut(s) | 626, 900 |
| BstV1I | GCAGC | 6 cut(s) | 967, 1044, 1666, 1903, 2379, 2644 |
| BstV2I | GAAGAC | 3 cut(s) | 1685, 2677, 2868 |
| BstX2I | RGATCY | 4 cut(s) | 1299, 1705, 2298, 2758 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 400 |
| BstYI | RGATCY | 4 cut(s) | 1299, 1705, 2298, 2758 |
| BstZ17I | GTATAC | 1 cut(s) | 2161 |
| BsuRI | GGCC | 3 cut(s) | 645, 789, 1464 |
| BtsCI | GGATG | 4 cut(s) | 555, 611, 1858, 1982 |
| BtsI | GCAGTG | 1 cut(s) | 2058 |
| BtsIMutI | CAGTG | 2 cut(s) | 588, 2058 |
| BveI | ACCTGC | 1 cut(s) | 2803 |
| Cac8I | GCNNGC | 2 cut(s) | 1903, 2944 |
| CciI | TCATGA | 1 cut(s) | 2479 |
| CfoI | GCGC | 1 cut(s) | 437 |
| Cfr10I | RCCGGY | 1 cut(s) | 476 |
| Cfr13I | GGNCC | 2 cut(s) | 2209, 2986 |
| Csp6I | GTAC | 5 cut(s) | 238, 390, 1515, 1808, 1916 |
| CspCI | CAANNNNNGTGG | 6 cut(s) | 132, 167, 549, 584, 1852, 1887 |
| CviQI | GTAC | 5 cut(s) | 238, 390, 1515, 1808, 1916 |
| DraI | TTTAAA | 2 cut(s) | 1275, 2692 |
| DrdI | GACNNNNNNGTC | 2 cut(s) | 375, 381 |
| DseDI | GACNNNNNNGTC | 2 cut(s) | 375, 381 |
| Eam1104I | CTCTTC | 5 cut(s) | 1989, 2734, 2772, 2778, 2976 |
| EarI | CTCTTC | 5 cut(s) | 1989, 2734, 2772, 2778, 2976 |
| EciI | GGCGGA | 1 cut(s) | 765 |
| Eco130I | CCWWGG | 6 cut(s) | 567, 1129, 1639, 1674, 1965, 2960 |
| Eco147I | AGGCCT | 2 cut(s) | 789, 1464 |
| Eco31I | GGTCTC | 1 cut(s) | 683 |
| Eco32I | GATATC | 1 cut(s) | 1564 |
| Eco47I | GGWCC | 2 cut(s) | 2209, 2986 |
| Eco57I | CTGAAG | 4 cut(s) | 292, 474, 1562, 1679 |
| EcoNI | CCTNNNNNAGG | 3 cut(s) | 298, 783, 2075 |
| EcoO109I | RGGNCCY | 1 cut(s) | 2209 |
| EcoRI | GAATTC | 2 cut(s) | 411, 1590 |
| EcoRII | CCWGG | 4 cut(s) | 778, 1200, 2662, 2734 |
| EcoRV | GATATC | 1 cut(s) | 1564 |
| EcoT14I | CCWWGG | 6 cut(s) | 567, 1129, 1639, 1674, 1965, 2960 |
| ErhI | CCWWGG | 6 cut(s) | 567, 1129, 1639, 1674, 1965, 2960 |
| Esp3I | CGTCTC | 1 cut(s) | 383 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 2157, 2189 |
| FaqI | GGGAC | 1 cut(s) | 317 |
| FauI | CCCGC | 1 cut(s) | 931 |
| FbaI | TGATCA | 1 cut(s) | 2176 |
| FblI | GTMKAC | 1 cut(s) | 2160 |
| Fnu4HI | GCNGC | 6 cut(s) | 956, 1033, 1680, 1892, 2393, 2658 |
| FokI | GGATG | 4 cut(s) | 542, 618, 1865, 1989 |
| Fsp4HI | GCNGC | 6 cut(s) | 956, 1033, 1680, 1892, 2393, 2658 |
| FspBI | CTAG | 7 cut(s) | 530, 641, 980, 1703, 1796, 2316, 2351 |
| FspI | TGCGCA | 1 cut(s) | 436 |
| GlaI | GCGC | 1 cut(s) | 436 |
| GluI | GCNGC | 6 cut(s) | 956, 1033, 1680, 1892, 2393, 2658 |
| GsaI | CCCAGC | 1 cut(s) | 1911 |
| GsuI | CTGGAG | 5 cut(s) | 791, 935, 1061, 1424, 2646 |
| HaeIII | GGCC | 3 cut(s) | 645, 789, 1464 |
| HapII | CCGG | 2 cut(s) | 477, 1584 |
| HhaI | GCGC | 1 cut(s) | 437 |
| Hin1I | GRCGYC | 1 cut(s) | 376 |
| Hin6I | GCGC | 1 cut(s) | 435 |
| HinP1I | GCGC | 1 cut(s) | 435 |
| HincII | GTYRAC | 3 cut(s) | 917, 1023, 3031 |
| HindII | GTYRAC | 3 cut(s) | 917, 1023, 3031 |
| HindIII | AAGCTT | 3 cut(s) | 512, 655, 1430 |
| HinfI | GANTC | 9 cut(s) | 170, 340, 574, 1156, 1659, 2115, 2583, 2603, 2954 |
| HpaII | CCGG | 2 cut(s) | 477, 1584 |
| HphI | GGTGA | 6 cut(s) | 264, 1315, 1420, 1814, 2576, 2949 |
| Hpy166II | GTNNAC | 9 cut(s) | 917, 1023, 1218, 1956, 2161, 2492, 2818, 2989, 3031 |
| Hpy8I | GTNNAC | 9 cut(s) | 917, 1023, 1218, 1956, 2161, 2492, 2818, 2989, 3031 |
| Hpy99I | CGWCG | 1 cut(s) | 378 |
| HpyCH4III | ACNGT | 3 cut(s) | 70, 1184, 2629 |
| HpyCH4IV | ACGT | 1 cut(s) | 376 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 477, 883, 1258, 1617 |
| HpySE526I | ACGT | 1 cut(s) | 376 |
| Hsp92I | GRCGYC | 1 cut(s) | 376 |
| HspAI | GCGC | 1 cut(s) | 435 |
| KpnI | GGTACC | 1 cut(s) | 1811 |
| Ksp22I | TGATCA | 1 cut(s) | 2176 |
| LmnI | GCTCC | 4 cut(s) | 551, 2011, 2231, 2665 |
| Lsp1109I | GCAGC | 6 cut(s) | 967, 1044, 1666, 1903, 2379, 2644 |
| LweI | GCATC | 4 cut(s) | 529, 747, 2736, 3004 |
| MaeI | CTAG | 7 cut(s) | 530, 641, 980, 1703, 1796, 2316, 2351 |
| MaeII | ACGT | 1 cut(s) | 376 |
| MaeIII | GTNAC | 3 cut(s) | 226, 1388, 2831 |
| MfeI | CAATTG | 1 cut(s) | 1328 |
| MflI | RGATCY | 4 cut(s) | 1299, 1705, 2298, 2758 |
| MhlI | GDGCHC | 3 cut(s) | 626, 900, 2897 |
| MlyI | GAGTC | 3 cut(s) | 164, 2577, 2597 |
| MmeI | TCCRAC | 4 cut(s) | 381, 391, 2611, 2945 |
| MroXI | GAANNNNTTC | 2 cut(s) | 988, 2070 |
| MseI | TTAA | 9 cut(s) | 452, 717, 762, 1187, 1274, 2214, 2691, 2882, 2928 |
| MslI | CAYNNNNRTG | 2 cut(s) | 2511, 2900 |
| MspCI | CTTAAG | 1 cut(s) | 2927 |
| MspI | CCGG | 2 cut(s) | 477, 1584 |
| MspR9I | CCNGG | 4 cut(s) | 780, 1202, 2664, 2736 |
| MunI | CAATTG | 1 cut(s) | 1328 |
| Mva1269I | GAATGC | 2 cut(s) | 1371, 2261 |
| MvaI | CCWGG | 4 cut(s) | 780, 1202, 2664, 2736 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 477, 883, 1258, 1617 |
| NlaIV | GGNNCC | 9 cut(s) | 265, 553, 625, 1301, 1646, 1809, 1942, 2288, 2644 |
| NmeAIII | GCCGAG | 1 cut(s) | 2547 |
| NsbI | TGCGCA | 1 cut(s) | 436 |
| NspI | RCATGY | 5 cut(s) | 400, 799, 1905, 2310, 2946 |
| NspV | TTCGAA | 1 cut(s) | 1828 |
| OliI | CACNNNNGTG | 1 cut(s) | 2900 |
| PaeI | GCATGC | 2 cut(s) | 1905, 2946 |
| PagI | TCATGA | 1 cut(s) | 2479 |
| PaqCI | CACCTGC | 1 cut(s) | 2803 |
| PceI | AGGCCT | 2 cut(s) | 789, 1464 |
| PciI | ACATGT | 2 cut(s) | 396, 2306 |
| PctI | GAATGC | 2 cut(s) | 1371, 2261 |
| PdmI | GAANNNNTTC | 2 cut(s) | 988, 2070 |
| PfeI | GAWTC | 6 cut(s) | 340, 574, 1156, 1659, 2115, 2954 |
| PflMI | CCANNNNNTGG | 3 cut(s) | 281, 1385, 1402 |
| PfoI | TCCNGGA | 1 cut(s) | 2662 |
| PkrI | GCNGC | 6 cut(s) | 957, 1034, 1681, 1893, 2394, 2659 |
| Ple19I | CGATCG | 1 cut(s) | 100 |
| PleI | GAGTC | 3 cut(s) | 164, 2577, 2597 |
| PpsI | GAGTC | 3 cut(s) | 164, 2577, 2597 |
| PpuMI | RGGWCCY | 1 cut(s) | 2209 |
| PscI | ACATGT | 2 cut(s) | 396, 2306 |
| PsiI | TTATAA | 1 cut(s) | 1062 |
| Psp5II | RGGWCCY | 1 cut(s) | 2209 |
| Psp6I | CCWGG | 4 cut(s) | 778, 1200, 2662, 2734 |
| PspFI | CCCAGC | 1 cut(s) | 1907 |
| PspGI | CCWGG | 4 cut(s) | 778, 1200, 2662, 2734 |
| PspN4I | GGNNCC | 9 cut(s) | 265, 553, 625, 1301, 1646, 1809, 1942, 2288, 2644 |
| PspPI | GGNCC | 2 cut(s) | 2209, 2986 |
| PspPPI | RGGWCCY | 1 cut(s) | 2209 |
| PstI | CTGCAG | 1 cut(s) | 2053 |
| PsuI | RGATCY | 4 cut(s) | 1299, 1705, 2298, 2758 |
| PvuI | CGATCG | 1 cut(s) | 100 |
| RsaI | GTAC | 5 cut(s) | 239, 391, 1516, 1809, 1917 |
| RsaNI | GTAC | 5 cut(s) | 238, 390, 1515, 1808, 1916 |
| RseI | CAYNNNNRTG | 2 cut(s) | 2511, 2900 |
| SaqAI | TTAA | 9 cut(s) | 452, 717, 762, 1187, 1274, 2214, 2691, 2882, 2928 |
| SatI | GCNGC | 6 cut(s) | 956, 1033, 1680, 1892, 2393, 2658 |
| Sau96I | GGNCC | 2 cut(s) | 2209, 2986 |
| SchI | GAGTC | 3 cut(s) | 164, 2577, 2597 |
| ScrFI | CCNGG | 4 cut(s) | 780, 1202, 2664, 2736 |
| SduI | GDGCHC | 3 cut(s) | 626, 900, 2897 |
| SfaNI | GCATC | 4 cut(s) | 529, 747, 2736, 3004 |
| SfcI | CTRYAG | 3 cut(s) | 1092, 1748, 2049 |
| SfuI | TTCGAA | 1 cut(s) | 1828 |
| SinI | GGWCC | 2 cut(s) | 2209, 2986 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 2511, 2900 |
| SmlI | CTYRAG | 2 cut(s) | 1195, 2927 |
| SmoI | CTYRAG | 2 cut(s) | 1195, 2927 |
| SphI | GCATGC | 2 cut(s) | 1905, 2946 |
| SseBI | AGGCCT | 2 cut(s) | 789, 1464 |
| SsiI | CCGC | 5 cut(s) | 344, 469, 776, 924, 2042 |
| SspI | AATATT | 3 cut(s) | 457, 1384, 2443 |
| SspMI | CTAG | 7 cut(s) | 530, 641, 980, 1703, 1796, 2316, 2351 |
| StuI | AGGCCT | 2 cut(s) | 789, 1464 |
| StyD4I | CCNGG | 4 cut(s) | 778, 1200, 2662, 2734 |
| StyI | CCWWGG | 6 cut(s) | 567, 1129, 1639, 1674, 1965, 2960 |
| TaaI | ACNGT | 3 cut(s) | 70, 1184, 2629 |
| TaiI | ACGT | 1 cut(s) | 379 |
| TaqI | TCGA | 5 cut(s) | 1560, 1828, 2064, 2679, 2847 |
| TfiI | GAWTC | 6 cut(s) | 340, 574, 1156, 1659, 2115, 2954 |
| Tru1I | TTAA | 9 cut(s) | 452, 717, 762, 1187, 1274, 2214, 2691, 2882, 2928 |
| Tru9I | TTAA | 9 cut(s) | 452, 717, 762, 1187, 1274, 2214, 2691, 2882, 2928 |
| TscAI | CASTG | 2 cut(s) | 595, 2058 |
| TseI | GCWGC | 6 cut(s) | 955, 1032, 1679, 1891, 2392, 2657 |
| TspGWI | ACGGA | 4 cut(s) | 163, 276, 1312, 2127 |
| TspRI | CASTG | 2 cut(s) | 595, 2058 |
| Van91I | CCANNNNNTGG | 3 cut(s) | 281, 1385, 1402 |
| Vha464I | CTTAAG | 1 cut(s) | 2927 |
| VpaK11BI | GGWCC | 2 cut(s) | 2209, 2986 |
| XagI | CCTNNNNNAGG | 3 cut(s) | 298, 783, 2075 |
| XapI | RAATTY | 8 cut(s) | 116, 411, 483, 937, 1276, 1590, 1625, 2878 |
| XbaI | TCTAGA | 3 cut(s) | 529, 1702, 1795 |
| XceI | RCATGY | 5 cut(s) | 400, 799, 1905, 2310, 2946 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 2245 |
| XmiI | GTMKAC | 1 cut(s) | 2160 |
| XmnI | GAANNNNTTC | 2 cut(s) | 988, 2070 |
| XspI | CTAG | 7 cut(s) | 530, 641, 980, 1703, 1796, 2316, 2351 |
| ZraI | GACGTC | 1 cut(s) | 377 |