RchiOBHm_Chr1g0369961
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0000248
Physical Location & Seq
Reverse (-)
59586482 .. 59589574
3093 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ59418

Sequence Viewer

Length: 2625 bp
ATGAAGCTTATGCATCTTAATTTTGAGGTAGATAATCTGATAGGAGGCATCCCACCTTCCATGGGAAATGTTTCATCAATCACTATACTTTCCCTGGCAGAGAACAATTTGGTGGGCAGCGTTCCAGAGGTGCTAGGCCGATTGAGAAGCTTATCATTCTTTGTAATTGGTCTCAATAATCTCTCTGGTTTGATCCCTCCCTCCCTTTTTAACAAATCATCTATGAACGCCTTCTCACTTTCATTTAATAAATTTAAGGGCAGTATTCCACGCCTAAACATGCCTAATCTCCAAATACTGTACCTAGCTGGAAATGAATTCTTTGGACAAATCCCAGCTTCACTTTCCAATGCTTCTCAGCTTCAAGCGCTTGATTTTGGAGAAAATAATTTTGTTGGCCAACTTCCCGTATGTTTTGGAAATTTTCCAAATCTCCAGAGGCTCAGATTAACTAAGAATAATCTAGGAAGTAATTCATCAAATGATTTGGGATTTATAAAATTCTTGACAAATTGCAGCAATCTGGAGACTATTTCTTTGAGTGTTAACAACTTTGGAGGTGTTTTACCCGATTCTGTAGCCAATCTCTCAACCCAACTGACTCAACTCTACCTTGGGGGCAATCAAATAGCGCGAACAATTCCTGAAACATTAGGAAATCTGAACAATTTAATATCCCTGGGCTTGGGAGAAAACTTGTTCACAGGTACCATTCCAGCTTCTATTGGGAAGTTACAAAAGCTGCAAATGTTACATTTATATTCCAATAGATTATCAGGCCGGATCCCATCTTCCTTAGGGAACCTCACCCAATTGTTTCAACTCGGTATGTTTGAAAATGAATTAGAAGGAAGCATTCCTCCAAATATTGGTGACTGCAAAAATCTGCAAATGATGGATATATCACACAATAAGCTTAGTGGAGATATACCATCACAGGTCATTGGTCTGTCCTCCTTCTCTCTCTTGCTCAACTTATCGCAAAACTCGCTTACTGGCATTCTGCCTGTGGAAGTGGGTAAGCTGAAGAATATCAATATACTGGACATCTTTGATAATTATTTAACCGGAGGGATTCCAAAAATTATTGGAGGCTGTCTGAGCCTTGGATTTCTTTACCTACAAGGGAACCACTTTCAAGGAATCATACCTTCTTCTTTGTTAGCTTTGAGAGGTCTTCAGTATCTAGATCTTTCACGAAACAACTTGTCAGGACATATTCCAAAAGACCTACAGAGGCTTCCATTCTTGAAATATTTGAACCTTTCGTCCAATAATCTGGAGGGTGAGGCACCAAAAGGAGGAGTTTTTCAAAATACAAGTGCAATATCATTGAATGGAAATAGCAAACTTTGTGGTGGTGTTTCGGAATTGCAGCTACCAGCATGCCCCGTCAAAGTCCCAAAGCAGAGAAAGTTGCACGACTTCAAACTAAAGTTCACAATTTCTTTAGTTGCTAGATGCTCTCTTCTGTTTGCAGTGATCATAGCTCTTTATTGGAGGAGAAAAACTCAAAAGACTAAACCGCTATCTCCAGTGTCATCAATCAAATTCCTTCCAAAGGTTTCATACCAGACACTTCATCAACCTACTAGCGGATTCTCTCTGAGTAATCAAATTGGATCAGGAGGTTTTGGTTCTGTATACAAAGGGATTATTGATCAAGAAGAAAACAATGTTGTTGCCATAAAGGTCCTCAACCTTCAAGAGAAAGGAGCTTCCAAGAGTTTCGTGGCAGAATGCAATGCACTGAGAAATATTCGGCACAGGAACCTTGTGAAGATCTTAACATGTTGCTCCAGCACAGATTACAATGGTAATGACTTCAAAGCTCTAGTTTTTGAGAATATGTCAAATGGAAGTCTGGAGGAGTGGCTGCATAAAGAAAACCAATCAAGGAGTTTGAACCTTCTTCAAAGACTGAATATTGCTGTTGATGTGGCTTCTGCATTGTGTTATCTTCATGACCATTGTGAACCACAAATCATTCACTGCGACATTAAGCCGAGCAACGTTCTTCTTGATGATGAAATGGTTGCTCGTGTTGGAGATTTTGGGTTAGCAAGACTCATCTCAACGACAATGGACTCCTCTCAAATTCAAAGTAGCACAGTTGGGTTAAAGGGAACAATTGGCTATGCTGCTCCAGAGTATGCGAGTGGTGTTGAGCCATCAAGACAAGGGGATGTATATAGTTATGGGGTGCTCCTGTTGCAAATGTTCACAGGAAGAAGACCGATCGACGAAATGTTTAAAGAGGATTTGAACCTCCATAACTTTGTCAAGATGACCATACCAAAAAGAGTGATGGAGATTGTAGATTATACTCTTCTTGCCACTTTAAAAGATAGAGCACCTGCAACATCACAAAATGTAGTGAACTACATCAGTGGTTACAATAATGAAATCGAAGCAGTTGAAGAAAACATTGACAATGAGAATTTAAGCAAGATGAACACTTATGTGTGGAAGTGCATACTTCCAACCCTTAAGATTGGACTTGCATGCTCGGAAGAATCACCAAGGAATAGAATGTCTATGGAGGAGGTCCACATGAAGCTACACCATATAAAAGATGCTTGCACTGGTGTTGACATCTGTCAAGAAAGGCCAGGAAGAAGCTGA

Protein Analysis

874

Amino Acids

95.98

Weight (kDa)

8.41

Isoelectric Point (pI)

42.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_8 PF13855 95 - 155 2.8e-06 Leucine rich repeat
LRR_14 PF23598 171 - 277 1.1e-07 Leucine-rich repeat region
LRR_8 PF13855 199 - 258 1.9e-07 Leucine rich repeat
LRR_8 PF13855 372 - 427 9.7e-10 Leucine rich repeat
Pkinase PF00069 535 - 762 8.1e-42 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 536 - 755 1.3e-41 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000248)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g31390 FvH4_5g31630 FvH4_5g31631 FvH4_7g24151 FvH4_7g24160 FvH4_7g24240 FvH4_7g24242 FvH4_7g24243 FvH4_7g24510 FvH4_7g24920 FvH4_7g24920 FvH4_7g24920 FvH4_7g29910
malus_domestica MD01G1131800.v1.1 MD01G1150000.v1.1 MD01G1151300.v1.1
prunus_persica Prupe.2G248300_v2.0.a1
pyrus_communis pycom01g16800
rosa_chinensis RchiOBHm_Chr1g0339821 RchiOBHm_Chr1g0345151 RchiOBHm_Chr1g0346851 RchiOBHm_Chr1g0351981 RchiOBHm_Chr1g0369731 RchiOBHm_Chr1g0369841 RchiOBHm_Chr1g0369861 RchiOBHm_Chr1g0369931 RchiOBHm_Chr1g0369961 RchiOBHm_Chr1g0370261 RchiOBHm_Chr1g0370281 RchiOBHm_Chr5g0076391 RchiOBHm_Chr6g0248051 RchiOBHm_Chr6g0248071
rosa_laevigata RLG00000002548 RLG00000013479 RLG00000027042 RLG00000027045 RLG00000027047 RLG00000027060 RLG00000027063 RLG00000027082 RLG00000027084 RLG00000027087 RLG00000027089 RLG00000027090 RLG00000027091 RLG00000027092 RLG00000027094 RLG00000027097 RLG00000027100 RLG00000027101 RLG00000027111 RLG00000028769 RLG00000028851 RLG00000029244 RLG00000030021
rosa_multiflora Rmu_co7968444.1_g000001 Rmu_co8060564.1_g000001 Rmu_co8098766.1_g000001 Rmu_co8172886.1_g000001 Rmu_co8291893.1_g000001 Rmu_co8327887.1_g000001 Rmu_co8406981.1_g000001 Rmu_co8449189.1_g000001 Rmu_co8470871.1_g000001 Rmu_sc0000756.1_g000006 Rmu_sc0001154.1_g000028 Rmu_sc0001565.1_g000055 Rmu_sc0001657.1_g000002 Rmu_sc0002095.1_g000014 Rmu_sc0002147.1_g000001 Rmu_sc0002705.1_g000004 Rmu_sc0002705.1_g000005 Rmu_sc0004087.1_g000025 Rmu_sc0005500.1_g000022 Rmu_sc0006707.1_g000005 Rmu_sc0006707.1_g000009 Rmu_sc0008140.1_g000012 Rmu_sc0008140.1_g000019 Rmu_sc0016176.1_g000001 Rmu_sc0016906.1_g000006 Rmu_sc0028980.1_g000001 Rmu_sc0041369.1_g000001
rosa_roxburghii Rroxscaffold_3G00232610 Rroxscaffold_4G00286690 Rroxscaffold_4G00286920 Rroxscaffold_4G00286930 Rroxscaffold_4G00286960 Rroxscaffold_4G00286980 Rroxscaffold_4G00287030 Rroxscaffold_4G00287040 Rroxscaffold_4G00287070 Rroxscaffold_4G00287090 Rroxscaffold_4G00287330 Rroxscaffold_4G00287430 Rroxscaffold_4G00303380 Rroxscaffold_4G00303400 Rroxscaffold_4G00303410 Rroxscaffold_4G00308100 Rroxscaffold_4G00308480 Rroxscaffold_4G00313700 Rroxscaffold_4G00322910 Rroxscaffold_7G00190680 Rroxscaffold_7G00193490
rosa_rugosa Rorug01G0032200 Rorug01G0139400.1 Rorug01G0139600.1 Rorug01G0139700.1 Rorug01G0184400 Rorug01G0351900 Rorug01G0351900 Rorug01G0352600 Rorug01G0352600 Rorug01G0352800 Rorug01G0355300 Rorug01G0355400 Rorug01G0356700.1 Rorug01G0356800 Rorug06G0090600 Rorug07G0276100
rosa_samantha Rh1AG201900 Rh1AG361300 Rh1AG365100 Rh6AG204000
rosa_wichuraiana Rw0G015480 Rw1G007000 Rw1G008190 Rw1G012880 Rw1G012990 Rw1G016950 Rw1G031720 Rw1G031800 Rw1G031820 Rw1G032020 Rw1G032180 Rw6G017720 Rw7G035600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 497
AarI CACCTGC 1 cut(s) 2365
Acc36I ACCTGC 1 cut(s) 2365
Acc65I GGTACC 1 cut(s) 707
AccB1I GGYRCC 2 cut(s) 707, 1291
AccB7I CCANNNNNTGG 1 cut(s) 869
AccI GTMKAC 1 cut(s) 1644
AccII CGCG 1 cut(s) 634
AciI CCGC 2 cut(s) 1526, 1596
AclI AACGTT 1 cut(s) 2013
AclWI GGATC 4 cut(s) 187, 778, 791, 1630
AcoI YGGCCR 1 cut(s) 397
AcsI RAATTY 7 cut(s) 251, 317, 421, 500, 1550, 2097, 2440
AcuI CTGAAG 2 cut(s) 1046, 1163
AfaI GTAC 2 cut(s) 302, 709
AfeI AGCGCT 1 cut(s) 369
AfiI CCNNNNNNNGG 6 cut(s) 62, 685, 869, 1301, 1561, 1595
AflII CTTAAG 1 cut(s) 2489
AflIII ACRYGT 1 cut(s) 1790
AjnI CCWGG 3 cut(s) 93, 678, 2611
AleI CACNNNNGTG 1 cut(s) 2462
AloI GAACNNNNNNTCC 2 cut(s) 1253, 1285
Alw21I GWGCWC 2 cut(s) 2208, 2356
Alw26I GTCTC 2 cut(s) 176, 521
AlwI GGATC 4 cut(s) 187, 778, 791, 1630
Aor51HI AGCGCT 1 cut(s) 369
AoxI GGCC 4 cut(s) 136, 397, 778, 2609
ApeKI GCWGC 6 cut(s) 117, 516, 742, 1375, 1876, 2141
ApoI RAATTY 7 cut(s) 251, 317, 421, 500, 1550, 2097, 2440
ArsI GACNNNNNNTTYG 2 cut(s) 520, 552
Asp700I GAANNNNTTC 3 cut(s) 70, 230, 472
Asp718I GGTACC 1 cut(s) 707
AspLEI GCGC 2 cut(s) 370, 634
AspS9I GGNCC 2 cut(s) 1693, 2548
AsuHPI GGTGA 4 cut(s) 799, 884, 1298, 2511
AvaII GGWCC 2 cut(s) 1693, 2548
AxyI CCTNAGG 1 cut(s) 796
BalI TGGCCA 1 cut(s) 399
BamHI GGATCC 1 cut(s) 783
BanI GGYRCC 2 cut(s) 707, 1291
BauI CACGAG 1 cut(s) 2040
BbsI GAAGAC 2 cut(s) 1169, 2239
Bbv12I GWGCWC 2 cut(s) 2208, 2356
BbvI GCAGC 6 cut(s) 129, 528, 729, 1387, 1863, 2128
BccI CCATC 5 cut(s) 796, 889, 940, 2179, 2302
BciT130I CCWGG 3 cut(s) 95, 680, 2613
BclI TGATCA 2 cut(s) 1482, 1660
BcoDI GTCTC 2 cut(s) 176, 521
BfaI CTAG 7 cut(s) 134, 305, 464, 1187, 1458, 1593, 1835
BfmI CTRYAG 2 cut(s) 576, 1232
BfoI RGCGCY 1 cut(s) 371
BfrI CTTAAG 1 cut(s) 2489
BfuAI ACCTGC 1 cut(s) 2365
BglII AGATCT 2 cut(s) 1189, 1782
BisI GCNGC 6 cut(s) 118, 517, 743, 1376, 1877, 2142
BlsI GCNGC 6 cut(s) 119, 518, 744, 1377, 1878, 2143
Bme1390I CCNGG 3 cut(s) 95, 680, 2613
Bme18I GGWCC 2 cut(s) 1693, 2548
BmgT120I GGNCC 2 cut(s) 1693, 2548
BmiI GGNNCC 6 cut(s) 709, 785, 803, 1130, 1293, 1772
BmrFI CCNGG 3 cut(s) 95, 680, 2613
BmsI GCATC 4 cut(s) 22, 57, 1451, 2566
BoxI GACNNNNGTC 1 cut(s) 2598
BpiI GAAGAC 2 cut(s) 1169, 2239
BplI GAGNNNNNCTC 2 cut(s) 1495, 1527
BpmI CTGGAG 7 cut(s) 419, 545, 1301, 1518, 1783, 1886, 2130
BsaI GGTCTC 1 cut(s) 176
BsaJI CCNNGG 7 cut(s) 60, 93, 613, 678, 679, 1105, 2522
BsaWI WCCGGW 1 cut(s) 1067
Bsc4I CCNNNNNNNGG 6 cut(s) 62, 685, 869, 1301, 1561, 1595
Bse1I ACTGG 4 cut(s) 1000, 1047, 1535, 2590
Bse21I CCTNAGG 1 cut(s) 796
Bse3DI GCAATG 1 cut(s) 1750
BseBI CCWGG 3 cut(s) 95, 680, 2613
BseDI CCNNGG 7 cut(s) 60, 93, 613, 678, 679, 1105, 2522
BseGI GGATG 2 cut(s) 48, 2191
BseLI CCNNNNNNNGG 6 cut(s) 62, 685, 869, 1301, 1561, 1595
BseMI GCAATG 1 cut(s) 1750
BseMII CTCAG 5 cut(s) 371, 457, 1091, 1598, 1742
BseNI ACTGG 4 cut(s) 1000, 1047, 1535, 2590
BseRI GAGGAG 5 cut(s) 1317, 1516, 1883, 2080, 2558
BseXI GCAGC 6 cut(s) 129, 528, 729, 1387, 1863, 2128
BseYI CCCAGC 1 cut(s) 334
Bsh1236I CGCG 1 cut(s) 634
Bsh1285I CGRYCG 1 cut(s) 2241
BshFI GGCC 4 cut(s) 138, 399, 780, 2611
BshNI GGYRCC 2 cut(s) 707, 1291
BsiEI CGRYCG 1 cut(s) 2241
BsiHKAI GWGCWC 2 cut(s) 2208, 2356
BsiSI CCGG 2 cut(s) 781, 1068
BslFI GGGAC 1 cut(s) 1385
BslI CCNNNNNNNGG 6 cut(s) 62, 685, 869, 1301, 1561, 1595
BsmAI GTCTC 2 cut(s) 176, 521
BsmFI GGGAC 1 cut(s) 1385
BsmI GAATGC 3 cut(s) 855, 999, 1745
BsnI GGCC 4 cut(s) 138, 399, 780, 2611
Bso31I GGTCTC 1 cut(s) 176
Bsp1286I GDGCHC 2 cut(s) 2208, 2356
Bsp143I GATC 8 cut(s) 192, 783, 1189, 1482, 1622, 1660, 1782, 2238
Bsp19I CCATGG 1 cut(s) 60
BspACI CCGC 2 cut(s) 1526, 1596
BspANI GGCC 4 cut(s) 138, 399, 780, 2611
BspCNI CTCAG 5 cut(s) 370, 456, 1092, 1599, 1743
BspFNI CGCG 1 cut(s) 634
BspHI TCATGA 1 cut(s) 1963
BspLI GGNNCC 6 cut(s) 709, 785, 803, 1130, 1293, 1772
BspMI ACCTGC 1 cut(s) 2365
BspPI GGATC 4 cut(s) 187, 778, 791, 1630
BspT107I GGYRCC 2 cut(s) 707, 1291
BspTI CTTAAG 1 cut(s) 2489
BspTNI GGTCTC 1 cut(s) 176
BsrDI GCAATG 1 cut(s) 1750
BsrI ACTGG 4 cut(s) 1000, 1047, 1535, 2590
BssECI CCNNGG 7 cut(s) 60, 93, 613, 678, 679, 1105, 2522
BssMI GATC 8 cut(s) 192, 783, 1189, 1482, 1622, 1660, 1782, 2238
BssNAI GTATAC 1 cut(s) 1645
BssSI CACGAG 1 cut(s) 2040
BssT1I CCWWGG 4 cut(s) 60, 613, 1105, 2522
Bst1107I GTATAC 1 cut(s) 1645
Bst2BI CACGAG 1 cut(s) 2040
Bst2UI CCWGG 3 cut(s) 95, 680, 2613
Bst4CI ACNGT 2 cut(s) 300, 2113
Bst6I CTCTTC 2 cut(s) 1473, 2334
BstAFI CTTAAG 1 cut(s) 2489
BstC8I GCNNGC 3 cut(s) 1387, 2506, 2581
BstDEI CTNAG 8 cut(s) 357, 443, 453, 796, 917, 1100, 1607, 1751
BstDSI CCRYGG 1 cut(s) 60
BstENI CCTNNNNNAGG 1 cut(s) 1559
BstF5I GGATG 2 cut(s) 48, 2191
BstFNI CGCG 1 cut(s) 634
BstH2I RGCGCY 1 cut(s) 371
BstHHI GCGC 2 cut(s) 370, 634
BstKTI GATC 8 cut(s) 195, 786, 1192, 1485, 1625, 1663, 1785, 2241
BstMAI GTCTC 2 cut(s) 176, 521
BstMBI GATC 8 cut(s) 192, 783, 1189, 1482, 1622, 1660, 1782, 2238
BstMCI CGRYCG 1 cut(s) 2241
BstMWI GCNNNNNNNGC 4 cut(s) 367, 988, 1101, 2212
BstNI CCWGG 3 cut(s) 95, 680, 2613
BstNSI RCATGY 4 cut(s) 283, 1389, 1794, 2508
BstPAI GACNNNNGTC 1 cut(s) 2598
BstSCI CCNGG 3 cut(s) 93, 678, 2611
BstSFI CTRYAG 2 cut(s) 576, 1232
BstUI CGCG 1 cut(s) 634
BstV1I GCAGC 6 cut(s) 129, 528, 729, 1387, 1863, 2128
BstV2I GAAGAC 2 cut(s) 1169, 2239
BstX2I RGATCY 3 cut(s) 783, 1189, 1782
BstXI CCANNNNNNTGG 1 cut(s) 1279
BstYI RGATCY 3 cut(s) 783, 1189, 1782
BstZ17I GTATAC 1 cut(s) 1645
Bsu36I CCTNAGG 1 cut(s) 796
BsuRI GGCC 4 cut(s) 138, 399, 780, 2611
BtgI CCRYGG 1 cut(s) 60
BtsCI GGATG 2 cut(s) 48, 2191
BtsI GCAGTG 2 cut(s) 1485, 1990
BtsIMutI CAGTG 6 cut(s) 1485, 1542, 1748, 1990, 2395, 2583
BveI ACCTGC 1 cut(s) 2365
Cac8I GCNNGC 3 cut(s) 1387, 2506, 2581
CciI TCATGA 1 cut(s) 1963
CfoI GCGC 2 cut(s) 370, 634
Cfr13I GGNCC 2 cut(s) 1693, 2548
Csp6I GTAC 2 cut(s) 301, 708
CspCI CAANNNNNGTGG 2 cut(s) 1336, 1371
CviAII CATG 7 cut(s) 61, 280, 1386, 1791, 1964, 2505, 2554
CviQI GTAC 2 cut(s) 301, 708
DdeI CTNAG 8 cut(s) 357, 443, 453, 796, 917, 1100, 1607, 1751
DpnI GATC 8 cut(s) 194, 785, 1191, 1484, 1624, 1662, 1784, 2240
DpnII GATC 8 cut(s) 192, 783, 1189, 1482, 1622, 1660, 1782, 2238
DraI TTTAAA 2 cut(s) 2254, 2343
EaeI YGGCCR 1 cut(s) 397
Eam1104I CTCTTC 2 cut(s) 1473, 2334
EarI CTCTTC 2 cut(s) 1473, 2334
Eco130I CCWWGG 4 cut(s) 60, 613, 1105, 2522
Eco31I GGTCTC 1 cut(s) 176
Eco47I GGWCC 2 cut(s) 1693, 2548
Eco47III AGCGCT 1 cut(s) 369
Eco57I CTGAAG 2 cut(s) 1046, 1163
Eco81I CCTNAGG 1 cut(s) 796
EcoNI CCTNNNNNAGG 1 cut(s) 1559
EcoO109I RGGNCCY 1 cut(s) 1693
EcoRI GAATTC 1 cut(s) 317
EcoRII CCWGG 3 cut(s) 93, 678, 2611
EcoT14I CCWWGG 4 cut(s) 60, 613, 1105, 2522
EcoT22I ATGCAT 1 cut(s) 15
ErhI CCWWGG 4 cut(s) 60, 613, 1105, 2522
FaeI CATG 7 cut(s) 64, 283, 1389, 1794, 1967, 2508, 2557
FaqI GGGAC 1 cut(s) 1385
FatI CATG 7 cut(s) 60, 279, 1385, 1790, 1963, 2504, 2553
FbaI TGATCA 2 cut(s) 1482, 1660
FblI GTMKAC 1 cut(s) 1644
Fnu4HI GCNGC 6 cut(s) 118, 517, 743, 1376, 1877, 2142
FokI GGATG 2 cut(s) 35, 2198
Fsp4HI GCNGC 6 cut(s) 118, 517, 743, 1376, 1877, 2142
FspBI CTAG 7 cut(s) 134, 305, 464, 1187, 1458, 1593, 1835
GlaI GCGC 2 cut(s) 369, 633
GluI GCNGC 6 cut(s) 118, 517, 743, 1376, 1877, 2142
GsaI CCCAGC 1 cut(s) 338
GsuI CTGGAG 7 cut(s) 419, 545, 1301, 1518, 1783, 1886, 2130
HaeII RGCGCY 1 cut(s) 371
HaeIII GGCC 4 cut(s) 138, 399, 780, 2611
HapII CCGG 2 cut(s) 781, 1068
HhaI GCGC 2 cut(s) 370, 634
Hin1II CATG 7 cut(s) 64, 283, 1389, 1794, 1967, 2508, 2557
Hin6I GCGC 2 cut(s) 368, 632
HinP1I GCGC 2 cut(s) 368, 632
HincII GTYRAC 2 cut(s) 547, 2593
HindII GTYRAC 2 cut(s) 547, 2593
HindIII AAGCTT 3 cut(s) 5, 148, 914
HinfI GANTC 8 cut(s) 572, 601, 1075, 1143, 1599, 2067, 2087, 2516
HpaI GTTAAC 1 cut(s) 547
HpaII CCGG 2 cut(s) 781, 1068
HphI GGTGA 4 cut(s) 799, 884, 1298, 2511
Hpy166II GTNNAC 9 cut(s) 547, 702, 1440, 1645, 1976, 2223, 2380, 2551, 2593
Hpy188I TCNGA 7 cut(s) 39, 446, 663, 1101, 1369, 1608, 2512
Hpy8I GTNNAC 9 cut(s) 547, 702, 1440, 1645, 1976, 2223, 2380, 2551, 2593
Hpy99I CGWCG 1 cut(s) 2246
HpyAV CCTTC 8 cut(s) 66, 241, 842, 967, 1161, 1565, 1712, 1919
HpyCH4III ACNGT 2 cut(s) 300, 2113
HpyCH4IV ACGT 1 cut(s) 2013
HpyF10VI GCNNNNNNNGC 4 cut(s) 367, 988, 1101, 2212
HpyF3I CTNAG 8 cut(s) 357, 443, 453, 796, 917, 1100, 1607, 1751
HpySE526I ACGT 1 cut(s) 2013
Hsp92II CATG 7 cut(s) 64, 283, 1389, 1794, 1967, 2508, 2557
HspAI GCGC 2 cut(s) 368, 632
KpnI GGTACC 1 cut(s) 711
Ksp22I TGATCA 2 cut(s) 1482, 1660
KspAI GTTAAC 1 cut(s) 547
Kzo9I GATC 8 cut(s) 192, 783, 1189, 1482, 1622, 1660, 1782, 2238
LmnI GCTCC 4 cut(s) 1715, 1802, 2149, 2211
Lsp1109I GCAGC 6 cut(s) 129, 528, 729, 1387, 1863, 2128
LweI GCATC 4 cut(s) 22, 57, 1451, 2566
MaeI CTAG 7 cut(s) 134, 305, 464, 1187, 1458, 1593, 1835
MaeII ACGT 1 cut(s) 2013
MaeIII GTNAC 4 cut(s) 732, 750, 872, 2393
MalI GATC 8 cut(s) 194, 785, 1191, 1484, 1624, 1662, 1784, 2240
MboI GATC 8 cut(s) 192, 783, 1189, 1482, 1622, 1660, 1782, 2238
MfeI CAATTG 2 cut(s) 812, 2130
MflI RGATCY 3 cut(s) 783, 1189, 1782
MhlI GDGCHC 2 cut(s) 2208, 2356
MlsI TGGCCA 1 cut(s) 399
MluNI TGGCCA 1 cut(s) 399
MlyI GAGTC 3 cut(s) 595, 2061, 2081
MmeI TCCRAC 2 cut(s) 2026, 2507
Mox20I TGGCCA 1 cut(s) 399
Mph1103I ATGCAT 1 cut(s) 15
MroXI GAANNNNTTC 3 cut(s) 70, 230, 472
MscI TGGCCA 1 cut(s) 399
MslI CAYNNNNRTG 1 cut(s) 2462
Msp20I TGGCCA 1 cut(s) 399
MspCI CTTAAG 1 cut(s) 2489
MspI CCGG 2 cut(s) 781, 1068
MspR9I CCNGG 3 cut(s) 95, 680, 2613
MunI CAATTG 2 cut(s) 812, 2130
Mva1269I GAATGC 3 cut(s) 855, 999, 1745
MvaI CCWGG 3 cut(s) 95, 680, 2613
MvnI CGCG 1 cut(s) 634
MwoI GCNNNNNNNGC 4 cut(s) 367, 988, 1101, 2212
NcoI CCATGG 1 cut(s) 60
NdeII GATC 8 cut(s) 192, 783, 1189, 1482, 1622, 1660, 1782, 2238
NlaIII CATG 7 cut(s) 64, 283, 1389, 1794, 1967, 2508, 2557
NlaIV GGNNCC 6 cut(s) 709, 785, 803, 1130, 1293, 1772
NmeAIII GCCGAG 1 cut(s) 2031
NmuCI GTSAC 1 cut(s) 872
NsiI ATGCAT 1 cut(s) 15
NspI RCATGY 4 cut(s) 283, 1389, 1794, 2508
OliI CACNNNNGTG 1 cut(s) 2462
PaeI GCATGC 2 cut(s) 1389, 2508
PagI TCATGA 1 cut(s) 1963
PaqCI CACCTGC 1 cut(s) 2365
PasI CCCWGGG 1 cut(s) 679
PciI ACATGT 1 cut(s) 1790
PctI GAATGC 3 cut(s) 855, 999, 1745
PdmI GAANNNNTTC 3 cut(s) 70, 230, 472
PfeI GAWTC 5 cut(s) 572, 1075, 1143, 1599, 2516
PflMI CCANNNNNTGG 1 cut(s) 869
PkrI GCNGC 6 cut(s) 119, 518, 744, 1377, 1878, 2143
Ple19I CGATCG 1 cut(s) 2241
PleI GAGTC 3 cut(s) 595, 2061, 2081
PpsI GAGTC 3 cut(s) 595, 2061, 2081
PpuMI RGGWCCY 1 cut(s) 1693
PscI ACATGT 1 cut(s) 1790
PshAI GACNNNNGTC 1 cut(s) 2598
PsiI TTATAA 1 cut(s) 497
Psp1406I AACGTT 1 cut(s) 2013
Psp5II RGGWCCY 1 cut(s) 1693
Psp6I CCWGG 3 cut(s) 93, 678, 2611
PspFI CCCAGC 1 cut(s) 334
PspGI CCWGG 3 cut(s) 93, 678, 2611
PspN4I GGNNCC 6 cut(s) 709, 785, 803, 1130, 1293, 1772
PspPI GGNCC 2 cut(s) 1693, 2548
PspPPI RGGWCCY 1 cut(s) 1693
PsuI RGATCY 3 cut(s) 783, 1189, 1782
PvuI CGATCG 1 cut(s) 2241
RsaI GTAC 2 cut(s) 302, 709
RsaNI GTAC 2 cut(s) 301, 708
RseI CAYNNNNRTG 1 cut(s) 2462
SatI GCNGC 6 cut(s) 118, 517, 743, 1376, 1877, 2142
Sau3AI GATC 8 cut(s) 192, 783, 1189, 1482, 1622, 1660, 1782, 2238
Sau96I GGNCC 2 cut(s) 1693, 2548
SchI GAGTC 3 cut(s) 595, 2061, 2081
ScrFI CCNGG 3 cut(s) 95, 680, 2613
SduI GDGCHC 2 cut(s) 2208, 2356
SfaNI GCATC 4 cut(s) 22, 57, 1451, 2566
SfcI CTRYAG 2 cut(s) 576, 1232
SinI GGWCC 2 cut(s) 1693, 2548
SmiMI CAYNNNNRTG 1 cut(s) 2462
SmlI CTYRAG 1 cut(s) 2489
SmoI CTYRAG 1 cut(s) 2489
SphI GCATGC 2 cut(s) 1389, 2508
SsiI CCGC 2 cut(s) 1526, 1596
SspI AATATT 4 cut(s) 868, 1256, 1759, 1927
SspMI CTAG 7 cut(s) 134, 305, 464, 1187, 1458, 1593, 1835
StyD4I CCNGG 3 cut(s) 93, 678, 2611
StyI CCWWGG 4 cut(s) 60, 613, 1105, 2522
TaaI ACNGT 2 cut(s) 300, 2113
TaiI ACGT 1 cut(s) 2016
TaqI TCGA 2 cut(s) 2241, 2409
TaqII GACCGA 1 cut(s) 2251
TfiI GAWTC 5 cut(s) 572, 1075, 1143, 1599, 2516
TscAI CASTG 6 cut(s) 1485, 1542, 1755, 1997, 2395, 2590
TseFI GTSAC 1 cut(s) 872
TseI GCWGC 6 cut(s) 117, 516, 742, 1375, 1876, 2141
Tsp45I GTSAC 1 cut(s) 872
TspRI CASTG 6 cut(s) 1485, 1542, 1755, 1997, 2395, 2590
Van91I CCANNNNNTGG 1 cut(s) 869
Vha464I CTTAAG 1 cut(s) 2489
VpaK11BI GGWCC 2 cut(s) 1693, 2548
XagI CCTNNNNNAGG 1 cut(s) 1559
XapI RAATTY 7 cut(s) 251, 317, 421, 500, 1550, 2097, 2440
XbaI TCTAGA 1 cut(s) 1186
XceI RCATGY 4 cut(s) 283, 1389, 1794, 2508
XcmI CCANNNNNNNNNTGG 1 cut(s) 1729
XmiI GTMKAC 1 cut(s) 1644
XmnI GAANNNNTTC 3 cut(s) 70, 230, 472
XspI CTAG 7 cut(s) 134, 305, 464, 1187, 1458, 1593, 1835
Zsp2I ATGCAT 1 cut(s) 15
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.