Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0000248

Physical Location & Seq

Genomic Coordinates Reverse (-)

25873660 .. 25875954

Gene Structure 2295 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1150000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 954 bp

ATGGGTTTTCATGTTCCGAGCTTGTGCTCAGTTCGGTCTGTATACCTTTTTGTGACGACCCTTCTCTTTGTTATGAATCTTTCTTCTGCAAATCCTTTTGGGAACAAAACTGATCGATTGGCTTTGCTCAGATTTAAAGAATCCATATCTGCTGATCCACTCGGCAGAAGGCATCAGAGAGTCGCAGCCTTGAACATACAAGGCTCTGACTTGCGAGGAACTATATCACCTCAAATTGGCAACCTCTCATTTCTTAGGTTTATCAACCTTCGAAACAACAGCTTCTCGGGAAAGATTCCACAGCAAATTGGTCATCTGTTCCGACTGCAGCATCTCAATCTTATTGACAACATTTTGGAGGGGGAAATTCCAGAGGGTGAGGTACCAAAAGAAGGCGCCTTTCAAAACAAGAGCATAATATCATTGGTTGGAAATACAAAGCTTTGTGGTGGTGTCTCGGAATTTCAGCTGCCAGCATGCCCCATCAAAGGATCAGAACCTAGAAAGTTCCTCGGTCCCAAACAAAAACTTATAATTGCTTTAGTGGTGGGATGTCCTATCCTGCTTTCCTTCTTGCTTGCACTTCACTGGAGGAGAAAACAGGAAAAGAAACCACCATCTGCAATCTCATCAATCAACATCCCTTCAAAGGTTTCGTATGAAATGCTTCATCGAGCTACCAGCGGATTCATTCCAAACGCTTTAATTGGATTAGGAAGTTTTGGTTCTGTATATAAAGGGATTCTTGAAAATGAAGAAAATGTAGTTGCTATAAAGGTCCTCAACCTTCAACAAAAAGGAGCTTCCAAGAGTTTCACGGCGGAATGCAATGCACTAAGAAATATCCGGCACAGGAATCTCGTTAAGATCTTAACATGTTGCTCCAGCACGGATTACAATGGCAACGAATTCAAAGCACTGGTTTTCGAATACATGTCAAATGGAAGTTTATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0007275 GO:0008150 GO:0009790 GO:0009791 GO:0009793 GO:0009888 GO:0010065 GO:0010067 GO:0010087 GO:0010154 GO:0022414 GO:0032501 GO:0032502 GO:0048316 GO:0048507 GO:0048508 GO:0048608 GO:0048731 GO:0048856 GO:0061458

KEGG Pathways

Metabolic & Signaling

ko04010 ko04064 ko04620 ko04624 ko04722 ko05133 ko05140 ko05142 ko05145 ko05152 ko05162 ko05169 map04010 map04064 map04620 map04624 map04722 map05133 map05140 map05142 map05145 map05152 map05162 map05169

Pfam Domains

Protein Families

LRRNT_2 LRR_1 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

318

Amino Acids

34.95

Weight (kDa)

9.75

Isoelectric Point (pI)

45.49

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	234 - 317	2e-12	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	235 - 317	1.1e-12	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000248)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_4g31390 FvH4_5g31630 FvH4_5g31631 FvH4_7g24151 FvH4_7g24160 FvH4_7g24240 FvH4_7g24242 FvH4_7g24243 FvH4_7g24510 FvH4_7g24920 FvH4_7g24920 FvH4_7g24920 FvH4_7g29910
malus_domestica	MD01G1131800.v1.1 MD01G1150000.v1.1 MD01G1151300.v1.1
prunus_persica	Prupe.2G248300_v2.0.a1
pyrus_communis	pycom01g16800
rosa_chinensis	RchiOBHm_Chr1g0339821 RchiOBHm_Chr1g0345151 RchiOBHm_Chr1g0346851 RchiOBHm_Chr1g0351981 RchiOBHm_Chr1g0369731 RchiOBHm_Chr1g0369841 RchiOBHm_Chr1g0369861 RchiOBHm_Chr1g0369931 RchiOBHm_Chr1g0369961 RchiOBHm_Chr1g0370261 RchiOBHm_Chr1g0370281 RchiOBHm_Chr5g0076391 RchiOBHm_Chr6g0248051 RchiOBHm_Chr6g0248071
rosa_laevigata	RLG00000002548 RLG00000013479 RLG00000027042 RLG00000027045 RLG00000027047 RLG00000027060 RLG00000027063 RLG00000027082 RLG00000027084 RLG00000027087 RLG00000027089 RLG00000027090 RLG00000027091 RLG00000027092 RLG00000027094 RLG00000027097 RLG00000027100 RLG00000027101 RLG00000027111 RLG00000028769 RLG00000028851 RLG00000029244 RLG00000030021
rosa_multiflora	Rmu_co7968444.1_g000001 Rmu_co8060564.1_g000001 Rmu_co8098766.1_g000001 Rmu_co8172886.1_g000001 Rmu_co8291893.1_g000001 Rmu_co8327887.1_g000001 Rmu_co8406981.1_g000001 Rmu_co8449189.1_g000001 Rmu_co8470871.1_g000001 Rmu_sc0000756.1_g000006 Rmu_sc0001154.1_g000028 Rmu_sc0001565.1_g000055 Rmu_sc0001657.1_g000002 Rmu_sc0002095.1_g000014 Rmu_sc0002147.1_g000001 Rmu_sc0002705.1_g000004 Rmu_sc0002705.1_g000005 Rmu_sc0004087.1_g000025 Rmu_sc0005500.1_g000022 Rmu_sc0006707.1_g000005 Rmu_sc0006707.1_g000009 Rmu_sc0008140.1_g000012 Rmu_sc0008140.1_g000019 Rmu_sc0016176.1_g000001 Rmu_sc0016906.1_g000006 Rmu_sc0028980.1_g000001 Rmu_sc0041369.1_g000001
rosa_roxburghii	Rroxscaffold_3G00232610 Rroxscaffold_4G00286690 Rroxscaffold_4G00286920 Rroxscaffold_4G00286930 Rroxscaffold_4G00286960 Rroxscaffold_4G00286980 Rroxscaffold_4G00287030 Rroxscaffold_4G00287040 Rroxscaffold_4G00287070 Rroxscaffold_4G00287090 Rroxscaffold_4G00287330 Rroxscaffold_4G00287430 Rroxscaffold_4G00303380 Rroxscaffold_4G00303400 Rroxscaffold_4G00303410 Rroxscaffold_4G00308100 Rroxscaffold_4G00308480 Rroxscaffold_4G00313700 Rroxscaffold_4G00322910 Rroxscaffold_7G00190680 Rroxscaffold_7G00193490
rosa_rugosa	Rorug01G0032200 Rorug01G0139400.1 Rorug01G0139600.1 Rorug01G0139700.1 Rorug01G0184400 Rorug01G0351900 Rorug01G0351900 Rorug01G0352600 Rorug01G0352600 Rorug01G0352800 Rorug01G0355300 Rorug01G0355400 Rorug01G0356700.1 Rorug01G0356800 Rorug06G0090600 Rorug07G0276100
rosa_samantha	Rh1AG201900 Rh1AG361300 Rh1AG365100 Rh6AG204000
rosa_wichuraiana	Rw0G015480 Rw1G007000 Rw1G008190 Rw1G012880 Rw1G012990 Rw1G016950 Rw1G031720 Rw1G031800 Rw1G031820 Rw1G032020 Rw1G032180 Rw6G017720 Rw7G035600

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	533
Acc65I	GGTACC	1 cut(s)	382
AccB1I	GGYRCC	2 cut(s)	382, 395
AccI	GTMKAC	1 cut(s)	42
AciI	CCGC	2 cut(s)	684, 821
AclWI	GGATC	2 cut(s)	149, 499
AcsI	RAATTY	3 cut(s)	366, 461, 908
AcyI	GRCGYC	1 cut(s)	396
AfaI	GTAC	1 cut(s)	384
AfiI	CCNNNNNNNGG	4 cut(s)	236, 392, 488, 649
AflIII	ACRYGT	2 cut(s)	875, 933
AgsI	TTSAA	6 cut(s)	193, 404, 648, 749, 791, 913
AluBI	AGCT	6 cut(s)	21, 282, 442, 469, 677, 803
AluI	AGCT	6 cut(s)	21, 282, 442, 469, 677, 803
Alw21I	GWGCWC	1 cut(s)	29
Alw26I	GTCTC	1 cut(s)	460
AlwI	GGATC	2 cut(s)	149, 499
Ama87I	CYCGRG	1 cut(s)	286
ApeKI	GCWGC	3 cut(s)	185, 328, 469
ApoI	RAATTY	3 cut(s)	366, 461, 908
Asp700I	GAANNNNTTC	1 cut(s)	666
Asp718I	GGTACC	1 cut(s)	382
AspLEI	GCGC	1 cut(s)	398
AspS9I	GGNCC	2 cut(s)	515, 778
AsuHPI	GGTGA	2 cut(s)	219, 389
AsuII	TTCGAA	2 cut(s)	271, 927
AvaI	CYCGRG	1 cut(s)	286
AvaII	GGWCC	2 cut(s)	515, 778
BanI	GGYRCC	2 cut(s)	382, 395
Bbv12I	GWGCWC	1 cut(s)	29
BbvI	GCAGC	3 cut(s)	197, 340, 456
BccI	CCATC	2 cut(s)	491, 625
BceAI	ACGGC	1 cut(s)	834
BcoDI	GTCTC	1 cut(s)	460
BfaI	CTAG	1 cut(s)	501
BfmI	CTRYAG	1 cut(s)	326
BfoI	RGCGCY	1 cut(s)	399
BglII	AGATCT	1 cut(s)	867
BisI	GCNGC	3 cut(s)	186, 329, 470
BlsI	GCNGC	3 cut(s)	187, 330, 471
Bme18I	GGWCC	2 cut(s)	515, 778
BmeT110I	CYCGRG	1 cut(s)	286
BmgT120I	GGNCC	2 cut(s)	515, 778
BmiI	GGNNCC	3 cut(s)	384, 397, 517
BmsI	GCATC	2 cut(s)	181, 340
BpmI	CTGGAG	2 cut(s)	610, 868
Bpu14I	TTCGAA	2 cut(s)	271, 927
Bsa29I	ATCGAT	1 cut(s)	115
BsaHI	GRCGYC	1 cut(s)	396
BsaJI	CCNNGG	1 cut(s)	511
Bsc4I	CCNNNNNNNGG	4 cut(s)	236, 392, 488, 649
Bse1I	ACTGG	2 cut(s)	593, 924
Bse3DI	GCAATG	1 cut(s)	835
BseCI	ATCGAT	1 cut(s)	115
BseDI	CCNNGG	1 cut(s)	511
BseGI	GGATG	2 cut(s)	557, 639
BseLI	CCNNNNNNNGG	4 cut(s)	236, 392, 488, 649
BseMI	GCAATG	1 cut(s)	835
BseMII	CTCAG	2 cut(s)	42, 142
BseNI	ACTGG	2 cut(s)	593, 924
BseRI	GAGGAG	1 cut(s)	607
BseXI	GCAGC	3 cut(s)	197, 340, 456
BshNI	GGYRCC	2 cut(s)	382, 395
BshVI	ATCGAT	1 cut(s)	115
BsiHKAI	GWGCWC	1 cut(s)	29
BsiHKCI	CYCGRG	1 cut(s)	286
BsiSI	CCGG	1 cut(s)	847
BslFI	GGGAC	1 cut(s)	501
BslI	CCNNNNNNNGG	4 cut(s)	236, 392, 488, 649
BsmAI	GTCTC	1 cut(s)	460
BsmFI	GGGAC	1 cut(s)	501
BsmI	GAATGC	1 cut(s)	830
BsoBI	CYCGRG	1 cut(s)	286
Bsp119I	TTCGAA	2 cut(s)	271, 927
Bsp1286I	GDGCHC	1 cut(s)	29
Bsp143I	GATC	4 cut(s)	112, 154, 491, 867
BspACI	CCGC	2 cut(s)	684, 821
BspCNI	CTCAG	2 cut(s)	41, 141
BspDI	ATCGAT	1 cut(s)	115
BspLI	GGNNCC	3 cut(s)	384, 397, 517
BspMAI	CTGCAG	1 cut(s)	330
BspPI	GGATC	2 cut(s)	149, 499
BspT104I	TTCGAA	2 cut(s)	271, 927
BspT107I	GGYRCC	2 cut(s)	382, 395
BsrDI	GCAATG	1 cut(s)	835
BsrI	ACTGG	2 cut(s)	593, 924
BssECI	CCNNGG	1 cut(s)	511
BssMI	GATC	4 cut(s)	112, 154, 491, 867
BssNAI	GTATAC	1 cut(s)	43
BssNI	GRCGYC	1 cut(s)	396
Bst1107I	GTATAC	1 cut(s)	43
BstACI	GRCGYC	1 cut(s)	396
BstBI	TTCGAA	2 cut(s)	271, 927
BstC8I	GCNNGC	3 cut(s)	474, 478, 579
BstDEI	CTNAG	4 cut(s)	28, 128, 254, 836
BstF5I	GGATG	2 cut(s)	557, 639
BstH2I	RGCGCY	1 cut(s)	399
BstHHI	GCGC	1 cut(s)	398
BstKTI	GATC	4 cut(s)	115, 157, 494, 870
BstMAI	GTCTC	1 cut(s)	460
BstMBI	GATC	4 cut(s)	112, 154, 491, 867
BstNSI	RCATGY	3 cut(s)	480, 879, 937
BstSFI	CTRYAG	1 cut(s)	326
BstV1I	GCAGC	3 cut(s)	197, 340, 456
BstX2I	RGATCY	1 cut(s)	867
BstYI	RGATCY	1 cut(s)	867
BstZ17I	GTATAC	1 cut(s)	43
Bsu15I	ATCGAT	1 cut(s)	115
BsuTUI	ATCGAT	1 cut(s)	115
BtsCI	GGATG	2 cut(s)	557, 639
BtsIMutI	CAGTG	2 cut(s)	586, 917
Cac8I	GCNNGC	3 cut(s)	474, 478, 579
CfoI	GCGC	1 cut(s)	398
Cfr13I	GGNCC	2 cut(s)	515, 778
ClaI	ATCGAT	1 cut(s)	115
Csp6I	GTAC	1 cut(s)	383
CviAII	CATG	4 cut(s)	11, 477, 876, 934
CviJI	RGCY	9 cut(s)	21, 122, 188, 204, 282, 442, 469, 677, 803
CviKI_1	RGCY	9 cut(s)	21, 122, 188, 204, 282, 442, 469, 677, 803
CviQI	GTAC	1 cut(s)	383
DdeI	CTNAG	4 cut(s)	28, 128, 254, 836
DinI	GGCGCC	1 cut(s)	397
DpnI	GATC	4 cut(s)	114, 156, 493, 869
DpnII	GATC	4 cut(s)	112, 154, 491, 867
DraI	TTTAAA	1 cut(s)	136
EciI	GGCGGA	1 cut(s)	836
Eco47I	GGWCC	2 cut(s)	515, 778
Eco88I	CYCGRG	1 cut(s)	286
EcoO109I	RGGNCCY	1 cut(s)	778
EcoRI	GAATTC	1 cut(s)	908
EgeI	GGCGCC	1 cut(s)	397
EheI	GGCGCC	1 cut(s)	397
FaeI	CATG	4 cut(s)	14, 480, 879, 937
FalI	AAGNNNNNCTT	2 cut(s)	729, 761
FaqI	GGGAC	1 cut(s)	501
FatI	CATG	4 cut(s)	10, 476, 875, 933
FblI	GTMKAC	1 cut(s)	42
Fnu4HI	GCNGC	3 cut(s)	186, 329, 470
FokI	GGATG	2 cut(s)	564, 626
Fsp4HI	GCNGC	3 cut(s)	186, 329, 470
FspBI	CTAG	1 cut(s)	501
GlaI	GCGC	1 cut(s)	397
GluI	GCNGC	3 cut(s)	186, 329, 470
GsuI	CTGGAG	2 cut(s)	610, 868
HaeII	RGCGCY	1 cut(s)	399
HapII	CCGG	1 cut(s)	847
HhaI	GCGC	1 cut(s)	398
Hin1I	GRCGYC	1 cut(s)	396
Hin1II	CATG	4 cut(s)	14, 480, 879, 937
Hin6I	GCGC	1 cut(s)	396
HinP1I	GCGC	1 cut(s)	396
HindIII	AAGCTT	1 cut(s)	440
HinfI	GANTC	7 cut(s)	76, 140, 180, 295, 687, 742, 856
HpaII	CCGG	1 cut(s)	847
HphI	GGTGA	2 cut(s)	219, 389
Hpy166II	GTNNAC	1 cut(s)	43
Hpy188I	TCNGA	7 cut(s)	18, 131, 177, 208, 323, 460, 496
Hpy188III	TCNNGA	3 cut(s)	288, 371, 746
Hpy8I	GTNNAC	1 cut(s)	43
HpyAV	CCTTC	7 cut(s)	71, 162, 278, 386, 580, 654, 797
HpyCH4V	TGCA	6 cut(s)	89, 328, 581, 623, 828, 833
HpyF3I	CTNAG	4 cut(s)	28, 128, 254, 836
Hsp92I	GRCGYC	1 cut(s)	396
Hsp92II	CATG	4 cut(s)	14, 480, 879, 937
HspAI	GCGC	1 cut(s)	396
KasI	GGCGCC	1 cut(s)	395
KpnI	GGTACC	1 cut(s)	386
Kzo9I	GATC	4 cut(s)	112, 154, 491, 867
LmnI	GCTCC	2 cut(s)	800, 887
Lsp1109I	GCAGC	3 cut(s)	197, 340, 456
LweI	GCATC	2 cut(s)	181, 340
MaeI	CTAG	1 cut(s)	501
MaeIII	GTNAC	1 cut(s)	52
MalI	GATC	4 cut(s)	114, 156, 493, 869
MboI	GATC	4 cut(s)	112, 154, 491, 867
MboII	GAAGA	2 cut(s)	75, 767
MflI	RGATCY	1 cut(s)	867
MhlI	GDGCHC	1 cut(s)	29
MluCI	AATT	7 cut(s)	234, 306, 366, 461, 534, 705, 908
Mly113I	GGCGCC	1 cut(s)	396
MlyI	GAGTC	1 cut(s)	189
MmeI	TCCRAC	2 cut(s)	346, 409
MnlI	CCTC	9 cut(s)	209, 240, 254, 352, 367, 373, 521, 585, 791
MroXI	GAANNNNTTC	1 cut(s)	666
MseI	TTAA	4 cut(s)	135, 704, 864, 872
MspA1I	CMGCKG	2 cut(s)	469, 684
MspI	CCGG	1 cut(s)	847
Mva1269I	GAATGC	1 cut(s)	830
NarI	GGCGCC	1 cut(s)	396
NdeII	GATC	4 cut(s)	112, 154, 491, 867
NlaIII	CATG	4 cut(s)	14, 480, 879, 937
NlaIV	GGNNCC	3 cut(s)	384, 397, 517
NmeAIII	GCCGAG	1 cut(s)	141
NmuCI	GTSAC	1 cut(s)	52
NspI	RCATGY	3 cut(s)	480, 879, 937
NspV	TTCGAA	2 cut(s)	271, 927
PaeI	GCATGC	1 cut(s)	480
PciI	ACATGT	2 cut(s)	875, 933
PctI	GAATGC	1 cut(s)	830
PdmI	GAANNNNTTC	1 cut(s)	666
PfeI	GAWTC	6 cut(s)	76, 140, 295, 687, 742, 856
PkrI	GCNGC	3 cut(s)	187, 330, 471
PleI	GAGTC	1 cut(s)	188
PluTI	GGCGCC	1 cut(s)	399
PpsI	GAGTC	1 cut(s)	188
PpuMI	RGGWCCY	1 cut(s)	778
PscI	ACATGT	2 cut(s)	875, 933
PsiI	TTATAA	1 cut(s)	533
Psp5II	RGGWCCY	1 cut(s)	778
PspN4I	GGNNCC	3 cut(s)	384, 397, 517
PspPI	GGNCC	2 cut(s)	515, 778
PspPPI	RGGWCCY	1 cut(s)	778
PstI	CTGCAG	1 cut(s)	330
PsuI	RGATCY	1 cut(s)	867
PvuII	CAGCTG	1 cut(s)	469
RsaI	GTAC	1 cut(s)	384
RsaNI	GTAC	1 cut(s)	383
SaqAI	TTAA	4 cut(s)	135, 704, 864, 872
SatI	GCNGC	3 cut(s)	186, 329, 470
Sau3AI	GATC	4 cut(s)	112, 154, 491, 867
Sau96I	GGNCC	2 cut(s)	515, 778
SchI	GAGTC	1 cut(s)	189
SduI	GDGCHC	1 cut(s)	29
SfaNI	GCATC	2 cut(s)	181, 340
SfcI	CTRYAG	1 cut(s)	326
SfoI	GGCGCC	1 cut(s)	397
SfuI	TTCGAA	2 cut(s)	271, 927
SinI	GGWCC	2 cut(s)	515, 778
SphI	GCATGC	1 cut(s)	480
Sse9I	AATT	7 cut(s)	234, 306, 366, 461, 534, 705, 908
SsiI	CCGC	2 cut(s)	684, 821
SspDI	GGCGCC	1 cut(s)	395
SspMI	CTAG	1 cut(s)	501
TaqI	TCGA	4 cut(s)	115, 271, 673, 927
TaqII	GACCGA	2 cut(s)	24, 503
TasI	AATT	7 cut(s)	234, 306, 366, 461, 534, 705, 908
TfiI	GAWTC	6 cut(s)	76, 140, 295, 687, 742, 856
Tru1I	TTAA	4 cut(s)	135, 704, 864, 872
Tru9I	TTAA	4 cut(s)	135, 704, 864, 872
TscAI	CASTG	2 cut(s)	593, 924
TseFI	GTSAC	1 cut(s)	52
TseI	GCWGC	3 cut(s)	185, 328, 469
Tsp45I	GTSAC	1 cut(s)	52
TspDTI	ATGAA	5 cut(s)	89, 659, 675, 679, 768
TspGWI	ACGGA	1 cut(s)	905
TspRI	CASTG	2 cut(s)	593, 924
VpaK11BI	GGWCC	2 cut(s)	515, 778
XapI	RAATTY	3 cut(s)	366, 461, 908
XceI	RCATGY	3 cut(s)	480, 879, 937
XmiI	GTMKAC	1 cut(s)	42
XmnI	GAANNNNTTC	1 cut(s)	666
XspI	CTAG	1 cut(s)	501

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.