RLG00000027087
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0000248
Physical Location & Seq
Forward (+)
8307315 .. 8310927
3613 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000027087

Sequence Viewer

Length: 3126 bp
ATGGAGAGTTTCACATACCTTCATGTCATCCTTTTCATCAACTTTTTACAACTCACCACTGTTGTAAGCTCATTTGGCAATGAAACAGATCGCATGGCTTTGCTAAAATTCAAAGATTGCATAGCCTCTGATCCACATGGGCTGTTGAACTCATGGAATAACTCCGTTCATTACTGCAAATGGCCTGGAATTACTTGTGGCAGACGGCATCAGAGAGTAACAGCCTTGTACCTACCACACGCTGTTTTGCACGGAACCATATCACCATACATTGGCAACCTCTCCTTTCTCAGGGACTTCAGCCTTTACAACAACAGCTTCTCTGGCAAGATTCCGCAACAAGTTGAACATTTATTCCGGCTTCGACATCTCAATCTCAGTATCAACATGTTAGAGGGGGAAATTCCAGTTAACCTGACCTTCTGTGCGGAACTGAGCATCATAAGTATTGGAAGAAACCGCCTTACCGGCAAAATTCCTTCAGAGATTGGCTCATTGATGAAGCTTGTGCATCTTAATTTTGAGGTAAATAATCTGATAGGAGGCCTCCCACCTTCCATGGGAAATCTTTCATCAATCACTGTACTTTCCTTGGTAGGGAACAATTTGGTGGGCACCGTTCCAGAGGCGCTAGGCCGATTGAGATGCTTATCAATCTTTGCAATTGGTCGAAATAATCTCTCTGGTTTGATCCCTCCCTCCCTTTTTAACATATCATCTATGACCGCCTTCTCAATTTCATTTAATAAATTTAAGGGCAGTATTCCACTCCTAAACATGCCTAATCTCCAAAAACTGTACCTCGCCGGAAATGAATTCTCTGGACAAATCCCAGCTTCACTTTCCAATGCTTCTCAGCTTCAAGCGCTTGATTTTGGGGTTAATAATTTTGTTGGCCAACTTCCTGTTAGTTTTGGAAATTTTCCAAATCTCCAGAGGCTCAGATTAACTAAGAATAATCTAGGAACTAATTCATCAAATGATTTAGGATTTATAACCTTCTTGACAAATTGCAGCGATCTGTGGCAGCTTGCTATGAGTTCTAACAATTTTGGAGGTGTTTTACCTAATTCTGTAGCCAATTTCTCAACCCAACTGACTCAACTCTACCTTGCAAGCAATCAAATAACGGGAACGATTCCTGAAACATTAGAAAATCTGAGCAATTTAATACTCTTGAGCTTGCAAGAAAACTTGTTCACAGGTACCATTCCAGCTTCTCTTGGGAAGTTACAAAAGCTGCAAATATTATATTTACATTCCAATAGATTATCAGGCCGGTTCCCATCTTCCTTAGGGAACCTCACCCAATTGTTTCAGCTCTATTTATTGGAAAATGAATTAGAAGGAAGCATTCCTCCAAATATTGGTAACTGCAAAAATCTGCAGGTGATGGATATATCACACAATAAGCTTAGTGGAGATATACCATCACAGGTCATTGGTCTGTCCTCCTTCTCTCTCTTGCTCAACTTATCACAAAACTCGCTAACTGGCATTTTGCCTGCGGAAGTGGGTAAGCTGAAGAACATCAATACACTGGACATCTCTGATAATAATTTGACCGGAGGAGTTCCAGAAATTATTGGAGGCTGTCTGAGCCTTGAATTTCTTTACCTACAAGGGAACCACTTTCAAGGAATCATACCTTCTTCTTTGGCTGCTTTGAGAGGTCTTCAGTTTTTAGATCTTTCACGAAACAACTTGTCAGGACATATTCCAAAAGACCTACAGAGGCTTCCATTCTTGAATTATTTGAACCTTTCGTCCAATAATCTGGAGGGTGAGGCACCGAAAGGAGGAGTTTTTCAAAACATAAGTGCAATATCATTGAATGGAAATACCAAACTTTGTGGTGGTGTTACGGAATTGCAGCTACCAGCATGCCTCATCAAAGTCCCAAAGCAGAGAAAGTTGCACGGCTTCAAAATAAAGTTCACAATTTCTTTAGTCGCTGGATGCTCTCTTCTGTTTGCAACAGTAATCACAGCTCTTTACTGGAGGGGAAAAACTCAAAAGAATAAACCGCTATCTGCAATGTCATCAATTAACTTCCTTCCAAAGGTTTCATACCAGACACTTCACCATGCCACTGGCGGATTCTCTCCAAGCAATCAAATTGGATCAGGCGGTTTTGGCTCTGTATACAAAGGGATTCTTAATCAAGAAGAAAACAATGTTGTTGCCATCAAGGTCCTCAACCTTCAACAAAAAGGTGCTTCCAAGAGTTTTGTGGCTGAATGCAATGCACTCAGAAATATCCGGCACAGGAATCTTGTGAAGATCTTAACATGTTGCTCCAGCACGGATTATAATGGTAATGACTTCAAGGCTCTAGTTTTTGAGTATATGTCAAATGGAAGTTTAGAGGAGTGGCTGTACAGAGTAAATCAATCAAGGAGTTTGAACCTTCTTCAAAGACTGAATATTGCTGTTGATGTGGCTTCTGCGTTGTGTTATCTTCATGATCATTGTGAACCAAAAGTCATTCACTGCGACATGAAGCCGAGCAATGTTCTTCTTGATAATGACATGGTTGCTCGTGTTGGTGATTTTGGGTTAGCAAGACTCATCTCAACGACCACGGACTCCTCTCAAAATCAGAGTAGCACGGTTGGAATAAAGGGAACCATTGGCTATGCTGCTCCAGAGTATGCGAGTGGTGTTGAGCCATCAAGACAAGGGGATGTATATAGTTTTGGGGTGCTTGTGTTGCAAATGTTCACAGGAAGAAGACCTATCGATGAAATATTTAAAGAGGGTTTGAACCTCCATAACTTTGTCAAGATGGCCATACCAGGAAGAGTGATGCAGATTGTAGATCCTAGTCTTCTGGCCACTTTAGAAGAGACAGCACCTGCAACATCTCAAAATATAGTGAACTACATCAGCAGTTACAATAACGAAATTGAAGCCGTTGAAGAAAACATTGACAATGAGAATTTAAGCAAGATGAACACTTATGTGTGGAAGTGCATACTTCCAACCCTTAAGATTGGACTTGCATGCTCGGAAGAATCACCAAGGAATAGAATGTCTATAGAGGAGGTCCACAGGAAGCTACACCATATAAAAGATGCTTACACTGGTGTTGACATCTGTCAAGAAAGGCCAAGAAGAAGCTGA

Protein Analysis

1042

Amino Acids

114.4

Weight (kDa)

8.65

Isoelectric Point (pI)

41.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 28 - 66 6.9e-11 Leucine rich repeat N-terminal domain
LRR_14 PF23598 91 - 320 5e-14 Leucine-rich repeat region
LRR_8 PF13855 365 - 424 4e-09 Leucine rich repeat
LRR_8 PF13855 490 - 545 1.7e-06 Leucine rich repeat
LRR_14 PF23598 491 - 592 2e-06 Leucine-rich repeat region
LRR_8 PF13855 535 - 593 2.9e-08 Leucine rich repeat
Pkinase PF00069 704 - 926 4.2e-42 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 705 - 918 3.8e-41 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000248)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g31390 FvH4_5g31630 FvH4_5g31631 FvH4_7g24151 FvH4_7g24160 FvH4_7g24240 FvH4_7g24242 FvH4_7g24243 FvH4_7g24510 FvH4_7g24920 FvH4_7g24920 FvH4_7g24920 FvH4_7g29910
malus_domestica MD01G1131800.v1.1 MD01G1150000.v1.1 MD01G1151300.v1.1
prunus_persica Prupe.2G248300_v2.0.a1
pyrus_communis pycom01g16800
rosa_chinensis RchiOBHm_Chr1g0339821 RchiOBHm_Chr1g0345151 RchiOBHm_Chr1g0346851 RchiOBHm_Chr1g0351981 RchiOBHm_Chr1g0369731 RchiOBHm_Chr1g0369841 RchiOBHm_Chr1g0369861 RchiOBHm_Chr1g0369931 RchiOBHm_Chr1g0369961 RchiOBHm_Chr1g0370261 RchiOBHm_Chr1g0370281 RchiOBHm_Chr5g0076391 RchiOBHm_Chr6g0248051 RchiOBHm_Chr6g0248071
rosa_laevigata RLG00000002548 RLG00000013479 RLG00000027042 RLG00000027045 RLG00000027047 RLG00000027060 RLG00000027063 RLG00000027082 RLG00000027084 RLG00000027087 RLG00000027089 RLG00000027090 RLG00000027091 RLG00000027092 RLG00000027094 RLG00000027097 RLG00000027100 RLG00000027101 RLG00000027111 RLG00000028769 RLG00000028851 RLG00000029244 RLG00000030021
rosa_multiflora Rmu_co7968444.1_g000001 Rmu_co8060564.1_g000001 Rmu_co8098766.1_g000001 Rmu_co8172886.1_g000001 Rmu_co8291893.1_g000001 Rmu_co8327887.1_g000001 Rmu_co8406981.1_g000001 Rmu_co8449189.1_g000001 Rmu_co8470871.1_g000001 Rmu_sc0000756.1_g000006 Rmu_sc0001154.1_g000028 Rmu_sc0001565.1_g000055 Rmu_sc0001657.1_g000002 Rmu_sc0002095.1_g000014 Rmu_sc0002147.1_g000001 Rmu_sc0002705.1_g000004 Rmu_sc0002705.1_g000005 Rmu_sc0004087.1_g000025 Rmu_sc0005500.1_g000022 Rmu_sc0006707.1_g000005 Rmu_sc0006707.1_g000009 Rmu_sc0008140.1_g000012 Rmu_sc0008140.1_g000019 Rmu_sc0016176.1_g000001 Rmu_sc0016906.1_g000006 Rmu_sc0028980.1_g000001 Rmu_sc0041369.1_g000001
rosa_roxburghii Rroxscaffold_3G00232610 Rroxscaffold_4G00286690 Rroxscaffold_4G00286920 Rroxscaffold_4G00286930 Rroxscaffold_4G00286960 Rroxscaffold_4G00286980 Rroxscaffold_4G00287030 Rroxscaffold_4G00287040 Rroxscaffold_4G00287070 Rroxscaffold_4G00287090 Rroxscaffold_4G00287330 Rroxscaffold_4G00287430 Rroxscaffold_4G00303380 Rroxscaffold_4G00303400 Rroxscaffold_4G00303410 Rroxscaffold_4G00308100 Rroxscaffold_4G00308480 Rroxscaffold_4G00313700 Rroxscaffold_4G00322910 Rroxscaffold_7G00190680 Rroxscaffold_7G00193490
rosa_rugosa Rorug01G0032200 Rorug01G0139400.1 Rorug01G0139600.1 Rorug01G0139700.1 Rorug01G0184400 Rorug01G0351900 Rorug01G0351900 Rorug01G0352600 Rorug01G0352600 Rorug01G0352800 Rorug01G0355300 Rorug01G0355400 Rorug01G0356700.1 Rorug01G0356800 Rorug06G0090600 Rorug07G0276100
rosa_samantha Rh1AG201900 Rh1AG361300 Rh1AG365100 Rh6AG204000
rosa_wichuraiana Rw0G015480 Rw1G007000 Rw1G008190 Rw1G012880 Rw1G012990 Rw1G016950 Rw1G031720 Rw1G031800 Rw1G031820 Rw1G032020 Rw1G032180 Rw6G017720 Rw7G035600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 995, 2313
AarI CACCTGC 2 cut(s) 1378, 2866
Acc36I ACCTGC 2 cut(s) 1378, 2866
Acc65I GGTACC 1 cut(s) 1205
AccB1I GGYRCC 3 cut(s) 614, 1205, 1789
AccB7I CCANNNNNTGG 2 cut(s) 272, 1367
AccI GTMKAC 1 cut(s) 2145
AciI CCGC 8 cut(s) 335, 428, 460, 726, 1508, 2027, 2097, 2130
AclWI GGATC 4 cut(s) 125, 685, 2131, 2816
AcoI YGGCCR 3 cut(s) 895, 2790, 2835
AcsI RAATTY 8 cut(s) 107, 402, 474, 749, 815, 919, 1607, 2941
AcuI CTGAAG 4 cut(s) 283, 465, 1544, 1661
AfaI GTAC 5 cut(s) 230, 585, 800, 1207, 2381
AfeI AGCGCT 1 cut(s) 867
AfiI CCNNNNNNNGG 7 cut(s) 272, 291, 560, 597, 1367, 1799, 2062
AflII CTTAAG 1 cut(s) 2990
AflIII ACRYGT 2 cut(s) 387, 2291
AjnI CCWGG 2 cut(s) 184, 2797
AleI CACNNNNGTG 1 cut(s) 2963
AloI GAACNNNNNNTCC 4 cut(s) 339, 371, 1751, 1783
Alw26I GTCTC 1 cut(s) 2843
AlwI GGATC 4 cut(s) 125, 685, 2131, 2816
AlwNI CAGNNNCTG 1 cut(s) 2858
Aor51HI AGCGCT 1 cut(s) 867
AoxI GGCC 8 cut(s) 182, 544, 634, 895, 1276, 2790, 2835, 3110
ApeKI GCWGC 6 cut(s) 1014, 1027, 1240, 1661, 1873, 2642
ApoI RAATTY 8 cut(s) 107, 402, 474, 749, 815, 919, 1607, 2941
Asp700I GAANNNNTTC 2 cut(s) 568, 970
Asp718I GGTACC 1 cut(s) 1205
AspLEI GCGC 2 cut(s) 631, 868
AspS9I GGNCC 2 cut(s) 2194, 3049
AsuHPI GGTGA 8 cut(s) 46, 255, 1297, 1402, 1796, 2075, 2561, 3012
AvaII GGWCC 2 cut(s) 2194, 3049
AxyI CCTNAGG 1 cut(s) 1294
BaeGI GKGCMC 1 cut(s) 617
BalI TGGCCA 3 cut(s) 897, 2792, 2837
BanI GGYRCC 3 cut(s) 614, 1205, 1789
BauI CACGAG 1 cut(s) 2541
BbsI GAAGAC 3 cut(s) 1667, 2740, 2822
BbvI GCAGC 6 cut(s) 1026, 1039, 1227, 1648, 1885, 2629
BccI CCATC 6 cut(s) 1294, 1387, 1438, 2195, 2680, 2782
BceAI ACGGC 3 cut(s) 221, 1936, 2900
BcgI CGANNNNNNTGC 2 cut(s) 627, 661
BciT130I CCWGG 2 cut(s) 186, 2799
BclI TGATCA 1 cut(s) 2467
BcoDI GTCTC 1 cut(s) 2843
BfaI CTAG 4 cut(s) 632, 962, 2336, 2826
BfmI CTRYAG 4 cut(s) 1074, 1385, 1730, 3039
BfoI RGCGCY 2 cut(s) 632, 869
BfrI CTTAAG 1 cut(s) 2990
BfuAI ACCTGC 2 cut(s) 1378, 2866
BglI GCCNNNNNGGC 1 cut(s) 468
BglII AGATCT 2 cut(s) 1687, 2283
BisI GCNGC 6 cut(s) 1015, 1028, 1241, 1662, 1874, 2643
BlsI GCNGC 6 cut(s) 1016, 1029, 1242, 1663, 1875, 2644
Bme1390I CCNGG 2 cut(s) 186, 2799
Bme18I GGWCC 2 cut(s) 2194, 3049
BmgT120I GGNCC 2 cut(s) 2194, 3049
BmiI GGNNCC 8 cut(s) 256, 616, 1207, 1283, 1301, 1628, 1791, 2629
BmrFI CCNGG 2 cut(s) 186, 2799
BmsI GCATC 7 cut(s) 217, 447, 520, 635, 1949, 2799, 3067
BoxI GACNNNNGTC 1 cut(s) 3099
BpiI GAAGAC 3 cut(s) 1667, 2740, 2822
BplI GAGNNNNNCTC 2 cut(s) 476, 508
BpmI CTGGAG 5 cut(s) 917, 1799, 2020, 2284, 2631
BpuEI CTTGAG 1 cut(s) 1198
Bsa29I ATCGAT 1 cut(s) 2742
BsaBI GATNNNNATC 1 cut(s) 649
BsaJI CCNNGG 4 cut(s) 558, 591, 2583, 3023
BsaWI WCCGGW 1 cut(s) 1565
BsaXI ACNNNNNCTCC 2 cut(s) 2363, 2393
Bsc4I CCNNNNNNNGG 7 cut(s) 272, 291, 560, 597, 1367, 1799, 2062
Bse118I RCCGGY 2 cut(s) 467, 1278
Bse1I ACTGG 6 cut(s) 407, 1498, 1545, 2003, 2098, 3091
Bse21I CCTNAGG 1 cut(s) 1294
Bse3DI GCAATG 4 cut(s) 85, 2043, 2251, 2518
Bse8I GATNNNNATC 1 cut(s) 649
BseBI CCWGG 2 cut(s) 186, 2799
BseCI ATCGAT 1 cut(s) 2742
BseDI CCNNGG 4 cut(s) 558, 591, 2583, 3023
BseGI GGATG 3 cut(s) 27, 1964, 2692
BseJI GATNNNNATC 1 cut(s) 649
BseLI CCNNNNNNNGG 7 cut(s) 272, 291, 560, 597, 1367, 1799, 2062
BseMI GCAATG 4 cut(s) 85, 2043, 2251, 2518
BseMII CTCAG 8 cut(s) 304, 391, 425, 869, 955, 1151, 1589, 2266
BseNI ACTGG 6 cut(s) 407, 1498, 1545, 2003, 2098, 3091
BseRI GAGGAG 5 cut(s) 1584, 1815, 2384, 2581, 3059
BseSI GKGCMC 1 cut(s) 617
BseXI GCAGC 6 cut(s) 1026, 1039, 1227, 1648, 1885, 2629
BseYI CCCAGC 1 cut(s) 832
BshFI GGCC 8 cut(s) 184, 546, 636, 897, 1278, 2792, 2837, 3112
BshNI GGYRCC 3 cut(s) 614, 1205, 1789
BshVI ATCGAT 1 cut(s) 2742
BsiSI CCGG 6 cut(s) 358, 468, 807, 1279, 1566, 2263
BslFI GGGAC 2 cut(s) 308, 1883
BslI CCNNNNNNNGG 7 cut(s) 272, 291, 560, 597, 1367, 1799, 2062
BsmAI GTCTC 1 cut(s) 2843
BsmFI GGGAC 2 cut(s) 308, 1883
BsmI GAATGC 2 cut(s) 1353, 2246
BsnI GGCC 8 cut(s) 184, 546, 636, 897, 1278, 2792, 2837, 3112
Bsp1286I GDGCHC 1 cut(s) 617
Bsp1407I TGTACA 1 cut(s) 2379
Bsp143I GATC 9 cut(s) 88, 130, 690, 1018, 1687, 2123, 2283, 2467, 2821
Bsp19I CCATGG 1 cut(s) 558
BspACI CCGC 8 cut(s) 335, 428, 460, 726, 1508, 2027, 2097, 2130
BspANI GGCC 8 cut(s) 184, 546, 636, 897, 1278, 2792, 2837, 3112
BspCNI CTCAG 8 cut(s) 303, 390, 426, 868, 954, 1152, 1590, 2265
BspDI ATCGAT 1 cut(s) 2742
BspHI TCATGA 1 cut(s) 2464
BspLI GGNNCC 8 cut(s) 256, 616, 1207, 1283, 1301, 1628, 1791, 2629
BspMAI CTGCAG 1 cut(s) 1389
BspMI ACCTGC 2 cut(s) 1378, 2866
BspPI GGATC 4 cut(s) 125, 685, 2131, 2816
BspT107I GGYRCC 3 cut(s) 614, 1205, 1789
BspTI CTTAAG 1 cut(s) 2990
BsrDI GCAATG 4 cut(s) 85, 2043, 2251, 2518
BsrFI RCCGGY 2 cut(s) 467, 1278
BsrGI TGTACA 1 cut(s) 2379
BsrI ACTGG 6 cut(s) 407, 1498, 1545, 2003, 2098, 3091
BssAI RCCGGY 2 cut(s) 467, 1278
BssECI CCNNGG 4 cut(s) 558, 591, 2583, 3023
BssMI GATC 9 cut(s) 88, 130, 690, 1018, 1687, 2123, 2283, 2467, 2821
BssNAI GTATAC 1 cut(s) 2146
BssSI CACGAG 1 cut(s) 2541
BssT1I CCWWGG 3 cut(s) 558, 591, 3023
Bst1107I GTATAC 1 cut(s) 2146
Bst2BI CACGAG 1 cut(s) 2541
Bst2UI CCWGG 2 cut(s) 186, 2799
Bst4CI ACNGT 6 cut(s) 61, 583, 619, 798, 1981, 2614
Bst6I CTCTTC 3 cut(s) 1971, 2797, 2841
BstAFI CTTAAG 1 cut(s) 2990
BstAUI TGTACA 1 cut(s) 2379
BstC8I GCNNGC 6 cut(s) 1032, 1117, 1184, 1506, 1885, 3007
BstDSI CCRYGG 2 cut(s) 558, 2583
BstENI CCTNNNNNAGG 2 cut(s) 289, 2060
BstF5I GGATG 3 cut(s) 27, 1964, 2692
BstH2I RGCGCY 2 cut(s) 632, 869
BstHHI GCGC 2 cut(s) 631, 868
BstKTI GATC 9 cut(s) 91, 133, 693, 1021, 1690, 2126, 2286, 2470, 2824
BstMAI GTCTC 1 cut(s) 2843
BstMBI GATC 9 cut(s) 88, 130, 690, 1018, 1687, 2123, 2283, 2467, 2821
BstMWI GCNNNNNNNGC 7 cut(s) 75, 324, 468, 865, 1599, 2136, 2713
BstNI CCWGG 2 cut(s) 186, 2799
BstNSI RCATGY 5 cut(s) 391, 781, 1887, 2295, 3009
BstPAI GACNNNNGTC 1 cut(s) 3099
BstSCI CCNGG 2 cut(s) 184, 2797
BstSFI CTRYAG 4 cut(s) 1074, 1385, 1730, 3039
BstSLI GKGCMC 1 cut(s) 617
BstV1I GCAGC 6 cut(s) 1026, 1039, 1227, 1648, 1885, 2629
BstV2I GAAGAC 3 cut(s) 1667, 2740, 2822
BstX2I RGATCY 3 cut(s) 1687, 2283, 2821
BstXI CCANNNNNNTGG 2 cut(s) 1777, 2093
BstYI RGATCY 3 cut(s) 1687, 2283, 2821
BstZ17I GTATAC 1 cut(s) 2146
Bsu15I ATCGAT 1 cut(s) 2742
Bsu36I CCTNAGG 1 cut(s) 1294
BsuRI GGCC 8 cut(s) 184, 546, 636, 897, 1278, 2792, 2837, 3112
BsuTUI ATCGAT 1 cut(s) 2742
BtgI CCRYGG 2 cut(s) 558, 2583
BtsCI GGATG 3 cut(s) 27, 1964, 2692
BtsI GCAGTG 1 cut(s) 2491
BtsIMutI CAGTG 6 cut(s) 57, 579, 1538, 2091, 2491, 3084
BveI ACCTGC 2 cut(s) 1378, 2866
Cac8I GCNNGC 6 cut(s) 1032, 1117, 1184, 1506, 1885, 3007
CaiI CAGNNNCTG 1 cut(s) 2858
CciI TCATGA 1 cut(s) 2464
CfoI GCGC 2 cut(s) 631, 868
Cfr10I RCCGGY 2 cut(s) 467, 1278
Cfr13I GGNCC 2 cut(s) 2194, 3049
ClaI ATCGAT 1 cut(s) 2742
Csp6I GTAC 5 cut(s) 229, 584, 799, 1206, 2380
CspCI CAANNNNNGTGG 2 cut(s) 1834, 1869
CviQI GTAC 5 cut(s) 229, 584, 799, 1206, 2380
DpnI GATC 9 cut(s) 90, 132, 692, 1020, 1689, 2125, 2285, 2469, 2823
DpnII GATC 9 cut(s) 88, 130, 690, 1018, 1687, 2123, 2283, 2467, 2821
DraI TTTAAA 1 cut(s) 2755
EaeI YGGCCR 3 cut(s) 895, 2790, 2835
Eam1104I CTCTTC 3 cut(s) 1971, 2797, 2841
EarI CTCTTC 3 cut(s) 1971, 2797, 2841
EciI GGCGGA 1 cut(s) 2112
Eco130I CCWWGG 3 cut(s) 558, 591, 3023
Eco147I AGGCCT 1 cut(s) 546
Eco47I GGWCC 2 cut(s) 2194, 3049
Eco47III AGCGCT 1 cut(s) 867
Eco57I CTGAAG 4 cut(s) 283, 465, 1544, 1661
Eco81I CCTNAGG 1 cut(s) 1294
EcoNI CCTNNNNNAGG 2 cut(s) 289, 2060
EcoO109I RGGNCCY 1 cut(s) 2194
EcoRI GAATTC 1 cut(s) 815
EcoRII CCWGG 2 cut(s) 184, 2797
EcoT14I CCWWGG 3 cut(s) 558, 591, 3023
ErhI CCWWGG 3 cut(s) 558, 591, 3023
FalI AAGNNNNNCTT 2 cut(s) 2142, 2174
FaqI GGGAC 2 cut(s) 308, 1883
FbaI TGATCA 1 cut(s) 2467
FblI GTMKAC 1 cut(s) 2145
Fnu4HI GCNGC 6 cut(s) 1015, 1028, 1241, 1662, 1874, 2643
FokI GGATG 3 cut(s) 14, 1971, 2699
Fsp4HI GCNGC 6 cut(s) 1015, 1028, 1241, 1662, 1874, 2643
FspBI CTAG 4 cut(s) 632, 962, 2336, 2826
GlaI GCGC 2 cut(s) 630, 867
GluI GCNGC 6 cut(s) 1015, 1028, 1241, 1662, 1874, 2643
GsaI CCCAGC 1 cut(s) 836
GsuI CTGGAG 5 cut(s) 917, 1799, 2020, 2284, 2631
HaeII RGCGCY 2 cut(s) 632, 869
HaeIII GGCC 8 cut(s) 184, 546, 636, 897, 1278, 2792, 2837, 3112
HapII CCGG 6 cut(s) 358, 468, 807, 1279, 1566, 2263
HhaI GCGC 2 cut(s) 631, 868
Hin6I GCGC 2 cut(s) 629, 866
HinP1I GCGC 2 cut(s) 629, 866
HincII GTYRAC 2 cut(s) 412, 3094
HindII GTYRAC 2 cut(s) 412, 3094
HindIII AAGCTT 2 cut(s) 503, 1412
HpaI GTTAAC 1 cut(s) 412
HpaII CCGG 6 cut(s) 358, 468, 807, 1279, 1566, 2263
HphI GGTGA 8 cut(s) 46, 255, 1297, 1402, 1796, 2075, 2561, 3012
Hpy166II GTNNAC 9 cut(s) 412, 1200, 1938, 2146, 2477, 2724, 2881, 3052, 3094
Hpy8I GTNNAC 9 cut(s) 412, 1200, 1938, 2146, 2477, 2724, 2881, 3052, 3094
HpyCH4III ACNGT 6 cut(s) 61, 583, 619, 798, 1981, 2614
HpyF10VI GCNNNNNNNGC 7 cut(s) 75, 324, 468, 865, 1599, 2136, 2713
HspAI GCGC 2 cut(s) 629, 866
KpnI GGTACC 1 cut(s) 1209
Ksp22I TGATCA 1 cut(s) 2467
KspAI GTTAAC 1 cut(s) 412
Kzo9I GATC 9 cut(s) 88, 130, 690, 1018, 1687, 2123, 2283, 2467, 2821
LmnI GCTCC 2 cut(s) 2303, 2650
Lsp1109I GCAGC 6 cut(s) 1026, 1039, 1227, 1648, 1885, 2629
LweI GCATC 7 cut(s) 217, 447, 520, 635, 1949, 2799, 3067
MaeI CTAG 4 cut(s) 632, 962, 2336, 2826
MaeIII GTNAC 5 cut(s) 217, 1230, 1370, 1861, 2894
MalI GATC 9 cut(s) 90, 132, 692, 1020, 1689, 2125, 2285, 2469, 2823
MboI GATC 9 cut(s) 88, 130, 690, 1018, 1687, 2123, 2283, 2467, 2821
MfeI CAATTG 2 cut(s) 663, 1310
MflI RGATCY 3 cut(s) 1687, 2283, 2821
MhlI GDGCHC 1 cut(s) 617
MlsI TGGCCA 3 cut(s) 897, 2792, 2837
MluNI TGGCCA 3 cut(s) 897, 2792, 2837
MlyI GAGTC 3 cut(s) 1093, 2562, 2582
MmeI TCCRAC 2 cut(s) 2596, 3008
Mox20I TGGCCA 3 cut(s) 897, 2792, 2837
MroXI GAANNNNTTC 2 cut(s) 568, 970
MscI TGGCCA 3 cut(s) 897, 2792, 2837
MslI CAYNNNNRTG 1 cut(s) 2963
Msp20I TGGCCA 3 cut(s) 897, 2792, 2837
MspCI CTTAAG 1 cut(s) 2990
MspI CCGG 6 cut(s) 358, 468, 807, 1279, 1566, 2263
MspR9I CCNGG 2 cut(s) 186, 2799
MunI CAATTG 2 cut(s) 663, 1310
Mva1269I GAATGC 2 cut(s) 1353, 2246
MvaI CCWGG 2 cut(s) 186, 2799
MwoI GCNNNNNNNGC 7 cut(s) 75, 324, 468, 865, 1599, 2136, 2713
NcoI CCATGG 1 cut(s) 558
NdeII GATC 9 cut(s) 88, 130, 690, 1018, 1687, 2123, 2283, 2467, 2821
NlaIV GGNNCC 8 cut(s) 256, 616, 1207, 1283, 1301, 1628, 1791, 2629
NmeAIII GCCGAG 1 cut(s) 2532
NspI RCATGY 5 cut(s) 391, 781, 1887, 2295, 3009
OliI CACNNNNGTG 1 cut(s) 2963
PaeI GCATGC 2 cut(s) 1887, 3009
PagI TCATGA 1 cut(s) 2464
PaqCI CACCTGC 2 cut(s) 1378, 2866
PceI AGGCCT 1 cut(s) 546
PciI ACATGT 2 cut(s) 387, 2291
PctI GAATGC 2 cut(s) 1353, 2246
PdmI GAANNNNTTC 2 cut(s) 568, 970
PfeI GAWTC 7 cut(s) 331, 1138, 1641, 2100, 2155, 2272, 3017
PflMI CCANNNNNTGG 2 cut(s) 272, 1367
PkrI GCNGC 6 cut(s) 1016, 1029, 1242, 1663, 1875, 2644
PleI GAGTC 3 cut(s) 1093, 2562, 2582
PpsI GAGTC 3 cut(s) 1093, 2562, 2582
PpuMI RGGWCCY 1 cut(s) 2194
PscI ACATGT 2 cut(s) 387, 2291
PshAI GACNNNNGTC 1 cut(s) 3099
PsiI TTATAA 2 cut(s) 995, 2313
Psp5II RGGWCCY 1 cut(s) 2194
Psp6I CCWGG 2 cut(s) 184, 2797
PspFI CCCAGC 1 cut(s) 832
PspGI CCWGG 2 cut(s) 184, 2797
PspN4I GGNNCC 8 cut(s) 256, 616, 1207, 1283, 1301, 1628, 1791, 2629
PspPI GGNCC 2 cut(s) 2194, 3049
PspPPI RGGWCCY 1 cut(s) 2194
PstI CTGCAG 1 cut(s) 1389
PstNI CAGNNNCTG 1 cut(s) 2858
PsuI RGATCY 3 cut(s) 1687, 2283, 2821
RsaI GTAC 5 cut(s) 230, 585, 800, 1207, 2381
RsaNI GTAC 5 cut(s) 229, 584, 799, 1206, 2380
RseI CAYNNNNRTG 1 cut(s) 2963
SatI GCNGC 6 cut(s) 1015, 1028, 1241, 1662, 1874, 2643
Sau3AI GATC 9 cut(s) 88, 130, 690, 1018, 1687, 2123, 2283, 2467, 2821
Sau96I GGNCC 2 cut(s) 2194, 3049
SchI GAGTC 3 cut(s) 1093, 2562, 2582
ScrFI CCNGG 2 cut(s) 186, 2799
SduI GDGCHC 1 cut(s) 617
SfaNI GCATC 7 cut(s) 217, 447, 520, 635, 1949, 2799, 3067
SfcI CTRYAG 4 cut(s) 1074, 1385, 1730, 3039
SinI GGWCC 2 cut(s) 2194, 3049
SmiMI CAYNNNNRTG 1 cut(s) 2963
SmlI CTYRAG 2 cut(s) 1177, 2990
SmoI CTYRAG 2 cut(s) 1177, 2990
SphI GCATGC 2 cut(s) 1887, 3009
SseBI AGGCCT 1 cut(s) 546
SsiI CCGC 8 cut(s) 335, 428, 460, 726, 1508, 2027, 2097, 2130
SspI AATATT 4 cut(s) 1248, 1366, 2428, 2751
SspMI CTAG 4 cut(s) 632, 962, 2336, 2826
StuI AGGCCT 1 cut(s) 546
StyD4I CCNGG 2 cut(s) 184, 2797
StyI CCWWGG 3 cut(s) 558, 591, 3023
TaaI ACNGT 6 cut(s) 61, 583, 619, 798, 1981, 2614
TaqI TCGA 3 cut(s) 364, 670, 2742
TatI WGTACW 2 cut(s) 583, 2379
TfiI GAWTC 7 cut(s) 331, 1138, 1641, 2100, 2155, 2272, 3017
TscAI CASTG 6 cut(s) 64, 586, 1545, 2098, 2498, 3091
TseI GCWGC 6 cut(s) 1014, 1027, 1240, 1661, 1873, 2642
TspGWI ACGGA 5 cut(s) 154, 267, 1880, 2321, 2600
TspRI CASTG 6 cut(s) 64, 586, 1545, 2098, 2498, 3091
Van91I CCANNNNNTGG 2 cut(s) 272, 1367
Vha464I CTTAAG 1 cut(s) 2990
VpaK11BI GGWCC 2 cut(s) 2194, 3049
XagI CCTNNNNNAGG 2 cut(s) 289, 2060
XapI RAATTY 8 cut(s) 107, 402, 474, 749, 815, 919, 1607, 2941
XceI RCATGY 5 cut(s) 391, 781, 1887, 2295, 3009
XcmI CCANNNNNNNNNTGG 1 cut(s) 2230
XmiI GTMKAC 1 cut(s) 2145
XmnI GAANNNNTTC 2 cut(s) 568, 970
XspI CTAG 4 cut(s) 632, 962, 2336, 2826
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.