Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000004

Orthogroup ID

OG0000248

Physical Location & Seq

Genomic Coordinates Forward (+)

29614835 .. 29619871

Gene Structure 5037 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_4G00308100.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2553 bp

ATGAACCGCTTCTCACTTTCAGCTAATAAATTTAAGGGCAGTATTCCTCCTGGTATAGGCCTAAACATGCCTAATCTCCAAGAAGTGTACCTTGGTACAAATGAATTCTCTGGCCAAATCCCAGCTTCATTTTCCAATGCTTCTCAGCTGCAGGTACTTGATGTTGGGGAAAATAATTTTGTTGGGCTAGTTCCCGCAAGTTTTGGAAATTTTCCCAATCTCCAGCTACTTAACTTCGAGGTCAATAATCTAGGAACTAATTCATCAAATGATTTGGGATTTATAACATTCTTGACAAATTGCAGCAATTTGTGGCTACTTTCTCTGAGTAATAACAATTTTGGAGGTGTTTTACCCAATTCTGTAGCCAATTTCTCAACCCAACTGACTCAACTCTGCCTTGGGGGCAATCAAATAGCAGGAACGATTCCTGAAACATTAGAAAATCTCAGCAATTTAATACTCCTGACCCTGGAAGAAAACTTGTTCACAGGTACCATTCCAGCTTCTTTTGGGAAGTTACAAAAGCTGCAAGTATTAAGTTTAGATTCCAATAGATTATCAGGCCAGATCCCATCTTCCTTAGGAAACCTCACCCAATTGTATCACCTCCACTTATTAGAAAATGAATTAGAAGGAAGCATTCCTCCAAATATTGGTAACTGCAAAAATCTGCAGCAATTGGATATATCAGACAATAAGCTTAGTGGAGATATACCATCACAGGTGATTAGTCTGTCCTTTTCTGTCTTGCTCAACTTGTCGCAAAACTCGCTGACTGGCATTCTACCTGTGGAAGTGGGTAAGCTAAAGAATGTCAATACACTGGACATCTCTGATAATAATTTGACCGGAGGAATTCCAGAAATTATTGGAGGCTGTTTGAGCCTTGAATTTCTTTACCTACAAGGGAACCACTTTCAAGGAATCGTACCTTCTTCTTTGGCTGCTTTGAGAGGTCTTCAGTATCTAGATCTTTCACGAAACAACTTGTCAGGACATATTCCAAAAGACCTACAGAGGCTTCCATTCTTGATCCATTTGAACCTTTCGTTCAATAATCTTGAGGGTGAGGTACCGAAAGAAAGAGTTTTCCAAAACACAAGTACAATATCATTGGATGGAAATACCCAACTTTGTGGAGGTATTTCAGAATTGCAGCTACCAGCATGCCCCATCAAAGTACCAAAGCAGAGAAAGTTGCATGCTTTCAAACTAAAGTTCACAATTTCCTTGGTCGCTGGATGCTCTCTTTTGTTTGCAGTCGTCTCAGCTCTTTATTGGAGGAGAAAAACTCAAAAGAAGAAGCCATTAATAGTAGTGTCATCGATTAATTTCCTTCCAAAGGTTTCATACCAGACACTTCATCATGCTACTGGCGGATTCTCTCCGAACAATCAAATCGGATCAGGCGGTTTTGGCTCTGTATACAAAGGGATTTTAAATCAAGAAGAAAACAACGTTGTTGCCATCAAGGTCCTCAACCTTCAACGGAAAGGTGCTTCCAAGAGTTTTGTGGCTGAATGCAATGCACTGAGAAATATCCGGCACAGGAATCTTGTGAAGATCTTAACATGTTGCTCCAGCACAGATTATAATGGTAATAACTTCAAAGCTCTAGTTTTTGAGTATATGTCAAATGGAAATTTAGAGGAGTGGCTGCACAGAGAAAATCAATCAACGAGTTTGAACCTTCTTCAAAGATTGAATATTGCTGTTGATGTGGCTTCTGCATTGTGTTATCTTCATGACTATTGTGAACCACAAATCATTCACTGTGACATGAAGCCGAGCAACATTCTTCTTGATGATGACATGGTTGCTCGTGTTGGTGATTTTGGGTTAGCAAGACTCATCTCAACAACCACGGACTCCTCTCAAAATCAAAGTAGCACAGTTAGAATAAAGGGAACCATTGGCTATGCTGCTCCAGAGTATGCGTGTGGTGTTGAGCCATCAAGAGAAGGAGATGTATATAGTTATGGGGTGCTCGTGCTGCAAATGTTCACAGGAAGAAGACCTATCGACGATATGTTTAAAGATGGTTTGAACCTCCATAACTTAGTCAAGATGGCCATACCAGAAACAGTGATGCAGATTGTAGATCCTACTCTTCTTACCACTTTAGAAGAGACAACACCTGCAACATCACAAAATGTAGTGAACTACATCAACTGTTACAATAATGAAATTGAAGCAGTTGAAGAAAACATTGACAATGAGAATTTAAGCAAGATGAACACTGTGTGGAAGTGCATACTTCCAACCCTTAAGATTGGACTTGCATGCTCGGAAGAATCACCAAGGAATAGAATGTCTATGGAGGAGGTCCACAGGAAGCTACACCATATAAAAGATGCTTACACTGGTGTTGATCAACATCTGTCAAGAAAGGCCAAGAAGAAGCTAAACTATAGTGTCAGTGAGAACATATTGGAGGGGGAGATTCCGGTTAACTTGAGCTACTACTATGAATTGAGCATCCTGAACTTCCAAACCATCGATCTAAAGAGAAAAATTCTGACAGAGCTTGGCTCATTGTTGAAGACTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0007275 GO:0008150 GO:0009790 GO:0009791 GO:0009793 GO:0009888 GO:0010065 GO:0010067 GO:0010087 GO:0010154 GO:0022414 GO:0032501 GO:0032502 GO:0048316 GO:0048507 GO:0048508 GO:0048608 GO:0048731 GO:0048856 GO:0061458

KEGG Pathways

Metabolic & Signaling

ko04010 ko04064 ko04620 ko04624 ko04722 ko05133 ko05140 ko05142 ko05145 ko05152 ko05162 ko05169 map04010 map04064 map04620 map04624 map04722 map05133 map05140 map05142 map05145 map05152 map05162 map05169

Pfam Domains

Protein Families

LRRNT_2 LRR_1 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

850

Amino Acids

93.74

Weight (kDa)

6.22

Isoelectric Point (pI)

39.88

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRR_8	PF13855	24 - 84	2e-07	Leucine rich repeat
LRR_14	PF23598	70 - 207	2.1e-07	Leucine-rich repeat region
LRR_8	PF13855	128 - 187	4.1e-08	Leucine rich repeat
LRR_14	PF23598	145 - 231	1.3e-08	Leucine-rich repeat region
LRR_8	PF13855	252 - 307	1.6e-06	Leucine rich repeat
LRR_14	PF23598	253 - 357	3.5e-07	Leucine-rich repeat region
LRR_8	PF13855	297 - 355	5e-09	Leucine rich repeat
Pkinase	PF00069	465 - 690	1.4e-42	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	465 - 681	1.5e-41	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0000248)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_4g31390 FvH4_5g31630 FvH4_5g31631 FvH4_7g24151 FvH4_7g24160 FvH4_7g24240 FvH4_7g24242 FvH4_7g24243 FvH4_7g24510 FvH4_7g24920 FvH4_7g24920 FvH4_7g24920 FvH4_7g29910
malus_domestica	MD01G1131800.v1.1 MD01G1150000.v1.1 MD01G1151300.v1.1
prunus_persica	Prupe.2G248300_v2.0.a1
pyrus_communis	pycom01g16800
rosa_chinensis	RchiOBHm_Chr1g0339821 RchiOBHm_Chr1g0345151 RchiOBHm_Chr1g0346851 RchiOBHm_Chr1g0351981 RchiOBHm_Chr1g0369731 RchiOBHm_Chr1g0369841 RchiOBHm_Chr1g0369861 RchiOBHm_Chr1g0369931 RchiOBHm_Chr1g0369961 RchiOBHm_Chr1g0370261 RchiOBHm_Chr1g0370281 RchiOBHm_Chr5g0076391 RchiOBHm_Chr6g0248051 RchiOBHm_Chr6g0248071
rosa_laevigata	RLG00000002548 RLG00000013479 RLG00000027042 RLG00000027045 RLG00000027047 RLG00000027060 RLG00000027063 RLG00000027082 RLG00000027084 RLG00000027087 RLG00000027089 RLG00000027090 RLG00000027091 RLG00000027092 RLG00000027094 RLG00000027097 RLG00000027100 RLG00000027101 RLG00000027111 RLG00000028769 RLG00000028851 RLG00000029244 RLG00000030021
rosa_multiflora	Rmu_co7968444.1_g000001 Rmu_co8060564.1_g000001 Rmu_co8098766.1_g000001 Rmu_co8172886.1_g000001 Rmu_co8291893.1_g000001 Rmu_co8327887.1_g000001 Rmu_co8406981.1_g000001 Rmu_co8449189.1_g000001 Rmu_co8470871.1_g000001 Rmu_sc0000756.1_g000006 Rmu_sc0001154.1_g000028 Rmu_sc0001565.1_g000055 Rmu_sc0001657.1_g000002 Rmu_sc0002095.1_g000014 Rmu_sc0002147.1_g000001 Rmu_sc0002705.1_g000004 Rmu_sc0002705.1_g000005 Rmu_sc0004087.1_g000025 Rmu_sc0005500.1_g000022 Rmu_sc0006707.1_g000005 Rmu_sc0006707.1_g000009 Rmu_sc0008140.1_g000012 Rmu_sc0008140.1_g000019 Rmu_sc0016176.1_g000001 Rmu_sc0016906.1_g000006 Rmu_sc0028980.1_g000001 Rmu_sc0041369.1_g000001
rosa_roxburghii	Rroxscaffold_3G00232610 Rroxscaffold_4G00286690 Rroxscaffold_4G00286920 Rroxscaffold_4G00286930 Rroxscaffold_4G00286960 Rroxscaffold_4G00286980 Rroxscaffold_4G00287030 Rroxscaffold_4G00287040 Rroxscaffold_4G00287070 Rroxscaffold_4G00287090 Rroxscaffold_4G00287330 Rroxscaffold_4G00287430 Rroxscaffold_4G00303380 Rroxscaffold_4G00303400 Rroxscaffold_4G00303410 Rroxscaffold_4G00308100 Rroxscaffold_4G00308480 Rroxscaffold_4G00313700 Rroxscaffold_4G00322910 Rroxscaffold_7G00190680 Rroxscaffold_7G00193490
rosa_rugosa	Rorug01G0032200 Rorug01G0139400.1 Rorug01G0139600.1 Rorug01G0139700.1 Rorug01G0184400 Rorug01G0351900 Rorug01G0351900 Rorug01G0352600 Rorug01G0352600 Rorug01G0352800 Rorug01G0355300 Rorug01G0355400 Rorug01G0356700.1 Rorug01G0356800 Rorug06G0090600 Rorug07G0276100
rosa_samantha	Rh1AG201900 Rh1AG361300 Rh1AG365100 Rh6AG204000
rosa_wichuraiana	Rw0G015480 Rw1G007000 Rw1G008190 Rw1G012880 Rw1G012990 Rw1G016950 Rw1G031720 Rw1G031800 Rw1G031820 Rw1G032020 Rw1G032180 Rw6G017720 Rw7G035600

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	284, 1596
AarI	CACCTGC	1 cut(s)	2149
Acc36I	ACCTGC	2 cut(s)	142, 2149
Acc65I	GGTACC	2 cut(s)	494, 1075
AccB1I	GGYRCC	2 cut(s)	494, 1075
AccB7I	CCANNNNNTGG	1 cut(s)	656
AccI	GTMKAC	1 cut(s)	1428
AciI	CCGC	4 cut(s)	7, 195, 1380, 1413
AclI	AACGTT	1 cut(s)	1461
AclWI	GGATC	4 cut(s)	565, 1030, 1414, 2099
AcoI	YGGCCR	2 cut(s)	112, 2073
AcsI	RAATTY	8 cut(s)	29, 104, 208, 858, 893, 1645, 2224, 2517
AcuI	CTGAAG	1 cut(s)	947
AdeI	CACNNNGTG	1 cut(s)	2247
AfaI	GTAC	8 cut(s)	89, 97, 156, 496, 933, 1077, 1108, 1185
AfiI	CCNNNNNNNGG	4 cut(s)	56, 472, 656, 1345
AflII	CTTAAG	1 cut(s)	2270
AflIII	ACRYGT	1 cut(s)	1574
AjnI	CCWGG	2 cut(s)	49, 471
AjuI	GAANNNNNNNTTGG	2 cut(s)	75, 107
Alw21I	GWGCWC	1 cut(s)	1992
Alw26I	GTCTC	2 cut(s)	1273, 2126
AlwI	GGATC	4 cut(s)	565, 1030, 1414, 2099
AoxI	GGCC	5 cut(s)	58, 112, 565, 2073, 2394
ApeKI	GCWGC	9 cut(s)	148, 303, 529, 676, 947, 1159, 1660, 1925, 1996
ApoI	RAATTY	8 cut(s)	29, 104, 208, 858, 893, 1645, 2224, 2517
AseI	ATTAAT	2 cut(s)	1313, 1332
Asp700I	GAANNNNTTC	2 cut(s)	8, 259
Asp718I	GGTACC	2 cut(s)	494, 1075
AspS9I	GGNCC	2 cut(s)	1477, 2329
AsuHPI	GGTGA	6 cut(s)	586, 599, 739, 1082, 1844, 2292
AvaII	GGWCC	2 cut(s)	1477, 2329
AxyI	CCTNAGG	1 cut(s)	583
BalI	TGGCCA	2 cut(s)	114, 2075
BanI	GGYRCC	2 cut(s)	494, 1075
BauI	CACGAG	2 cut(s)	1824, 1991
BbsI	GAAGAC	2 cut(s)	953, 2023
Bbv12I	GWGCWC	1 cut(s)	1992
BbvI	GCAGC	9 cut(s)	135, 315, 516, 688, 934, 1171, 1647, 1912, 1983
BccI	CCATC	9 cut(s)	583, 727, 1115, 1184, 1478, 1963, 2036, 2065, 2507
BciT130I	CCWGG	2 cut(s)	51, 473
BclI	TGATCA	1 cut(s)	2374
BcoDI	GTCTC	2 cut(s)	1273, 2126
BfaI	CTAG	4 cut(s)	188, 251, 971, 1619
BfmI	CTRYAG	5 cut(s)	149, 363, 674, 1016, 2413
BfrI	CTTAAG	1 cut(s)	2270
BfuAI	ACCTGC	2 cut(s)	142, 2149
BglI	GCCNNNNNGGC	1 cut(s)	405
BglII	AGATCT	2 cut(s)	973, 1566
BisI	GCNGC	9 cut(s)	149, 304, 530, 677, 948, 1160, 1661, 1926, 1997
BlsI	GCNGC	9 cut(s)	150, 305, 531, 678, 949, 1161, 1662, 1927, 1998
Bme1390I	CCNGG	2 cut(s)	51, 473
Bme18I	GGWCC	2 cut(s)	1477, 2329
BmgT120I	GGNCC	2 cut(s)	1477, 2329
BmiI	GGNNCC	4 cut(s)	496, 914, 1077, 1912
BmrFI	CCNGG	2 cut(s)	51, 473
BmsI	GCATC	4 cut(s)	1235, 2082, 2347, 2490
BpiI	GAAGAC	2 cut(s)	953, 2023
BplI	GAGNNNNNCTC	4 cut(s)	1279, 1311, 2519, 2551
BpmI	CTGGAG	3 cut(s)	206, 1567, 1914
BpuEI	CTTGAG	2 cut(s)	1085, 2479
Bsa29I	ATCGAT	2 cut(s)	1328, 2502
BsaBI	GATNNNNATC	1 cut(s)	2379
BsaJI	CCNNGG	6 cut(s)	91, 400, 471, 1233, 1866, 2303
BsaWI	WCCGGW	2 cut(s)	851, 2449
Bsc4I	CCNNNNNNNGG	4 cut(s)	56, 472, 656, 1345
Bse1I	ACTGG	4 cut(s)	784, 831, 1381, 2371
Bse21I	CCTNAGG	1 cut(s)	583
Bse3DI	GCAATG	1 cut(s)	1534
Bse8I	GATNNNNATC	1 cut(s)	2379
BseBI	CCWGG	2 cut(s)	51, 473
BseCI	ATCGAT	2 cut(s)	1328, 2502
BseDI	CCNNGG	6 cut(s)	91, 400, 471, 1233, 1866, 2303
BseGI	GGATG	3 cut(s)	1126, 1250, 2481
BseJI	GATNNNNATC	1 cut(s)	2379
BseLI	CCNNNNNNNGG	4 cut(s)	56, 472, 656, 1345
BseMI	GCAATG	1 cut(s)	1534
BseMII	CTCAG	5 cut(s)	158, 317, 463, 1284, 1526
BseNI	ACTGG	4 cut(s)	784, 831, 1381, 2371
BseRI	GAGGAG	4 cut(s)	1300, 1667, 1864, 2339
BseXI	GCAGC	9 cut(s)	135, 315, 516, 688, 934, 1171, 1647, 1912, 1983
BseYI	CCCAGC	1 cut(s)	121
BsgI	GTGCAG	1 cut(s)	1646
BshFI	GGCC	5 cut(s)	60, 114, 567, 2075, 2396
BshNI	GGYRCC	2 cut(s)	494, 1075
BshVI	ATCGAT	2 cut(s)	1328, 2502
BsiHKAI	GWGCWC	1 cut(s)	1992
BsiSI	CCGG	3 cut(s)	852, 1546, 2450
BslI	CCNNNNNNNGG	4 cut(s)	56, 472, 656, 1345
BsmAI	GTCTC	2 cut(s)	1273, 2126
BsmBI	CGTCTC	1 cut(s)	1273
BsmI	GAATGC	3 cut(s)	642, 783, 1529
BsnI	GGCC	5 cut(s)	60, 114, 567, 2075, 2396
Bsp1286I	GDGCHC	1 cut(s)	1992
Bsp143I	GATC	8 cut(s)	570, 973, 1035, 1406, 1566, 2104, 2374, 2503
BspACI	CCGC	4 cut(s)	7, 195, 1380, 1413
BspANI	GGCC	5 cut(s)	60, 114, 567, 2075, 2396
BspCNI	CTCAG	5 cut(s)	157, 318, 462, 1283, 1527
BspDI	ATCGAT	2 cut(s)	1328, 2502
BspHI	TCATGA	1 cut(s)	1747
BspLI	GGNNCC	4 cut(s)	496, 914, 1077, 1912
BspMAI	CTGCAG	2 cut(s)	153, 678
BspMI	ACCTGC	2 cut(s)	142, 2149
BspPI	GGATC	4 cut(s)	565, 1030, 1414, 2099
BspT107I	GGYRCC	2 cut(s)	494, 1075
BspTI	CTTAAG	1 cut(s)	2270
BsrDI	GCAATG	1 cut(s)	1534
BsrI	ACTGG	4 cut(s)	784, 831, 1381, 2371
BssECI	CCNNGG	6 cut(s)	91, 400, 471, 1233, 1866, 2303
BssMI	GATC	8 cut(s)	570, 973, 1035, 1406, 1566, 2104, 2374, 2503
BssNAI	GTATAC	1 cut(s)	1429
BssSI	CACGAG	2 cut(s)	1824, 1991
BssT1I	CCWWGG	4 cut(s)	91, 400, 1233, 2303
Bst1107I	GTATAC	1 cut(s)	1429
Bst2BI	CACGAG	2 cut(s)	1824, 1991
Bst2UI	CCWGG	2 cut(s)	51, 473
Bst4CI	ACNGT	5 cut(s)	1778, 1897, 2089, 2177, 2245
Bst6I	CTCTTC	2 cut(s)	2118, 2124
BstAFI	CTTAAG	1 cut(s)	2270
BstC8I	GCNNGC	3 cut(s)	1171, 1206, 2287
BstDEI	CTNAG	8 cut(s)	144, 326, 449, 583, 704, 1270, 1535, 2062
BstDSI	CCRYGG	1 cut(s)	1866
BstENI	CCTNNNNNAGG	2 cut(s)	54, 1343
BstF5I	GGATG	3 cut(s)	1126, 1250, 2481
BstKTI	GATC	8 cut(s)	573, 976, 1038, 1409, 1569, 2107, 2377, 2506
BstMAI	GTCTC	2 cut(s)	1273, 2126
BstMBI	GATC	8 cut(s)	570, 973, 1035, 1406, 1566, 2104, 2374, 2503
BstMWI	GCNNNNNNNGC	5 cut(s)	405, 772, 885, 1419, 1996
BstNI	CCWGG	2 cut(s)	51, 473
BstNSI	RCATGY	5 cut(s)	70, 1173, 1208, 1578, 2289
BstSCI	CCNGG	2 cut(s)	49, 471
BstSFI	CTRYAG	5 cut(s)	149, 363, 674, 1016, 2413
BstV1I	GCAGC	9 cut(s)	135, 315, 516, 688, 934, 1171, 1647, 1912, 1983
BstV2I	GAAGAC	2 cut(s)	953, 2023
BstX2I	RGATCY	4 cut(s)	570, 973, 1566, 2104
BstXI	CCANNNNNNTGG	1 cut(s)	1139
BstYI	RGATCY	4 cut(s)	570, 973, 1566, 2104
BstZ17I	GTATAC	1 cut(s)	1429
Bsu15I	ATCGAT	2 cut(s)	1328, 2502
Bsu36I	CCTNAGG	1 cut(s)	583
BsuRI	GGCC	5 cut(s)	60, 114, 567, 2075, 2396
BsuTUI	ATCGAT	2 cut(s)	1328, 2502
BtgI	CCRYGG	1 cut(s)	1866
BtsCI	GGATG	3 cut(s)	1126, 1250, 2481
BtsIMutI	CAGTG	7 cut(s)	824, 1532, 1774, 2094, 2241, 2364, 2428
BveI	ACCTGC	2 cut(s)	142, 2149
Cac8I	GCNNGC	3 cut(s)	1171, 1206, 2287
CciI	TCATGA	1 cut(s)	1747
Cfr13I	GGNCC	2 cut(s)	1477, 2329
ClaI	ATCGAT	2 cut(s)	1328, 2502
Csp6I	GTAC	8 cut(s)	88, 96, 155, 495, 932, 1076, 1107, 1184
CviAII	CATG	9 cut(s)	67, 1170, 1205, 1370, 1575, 1748, 1783, 1816, 2286
CviQI	GTAC	8 cut(s)	88, 96, 155, 495, 932, 1076, 1107, 1184
DdeI	CTNAG	8 cut(s)	144, 326, 449, 583, 704, 1270, 1535, 2062
DpnI	GATC	8 cut(s)	572, 975, 1037, 1408, 1568, 2106, 2376, 2505
DpnII	GATC	8 cut(s)	570, 973, 1035, 1406, 1566, 2104, 2374, 2503
DraI	TTTAAA	2 cut(s)	1443, 2038
DraIII	CACNNNGTG	1 cut(s)	2247
EaeI	YGGCCR	2 cut(s)	112, 2073
Eam1104I	CTCTTC	2 cut(s)	2118, 2124
EarI	CTCTTC	2 cut(s)	2118, 2124
EciI	GGCGGA	1 cut(s)	1395
Eco130I	CCWWGG	4 cut(s)	91, 400, 1233, 2303
Eco147I	AGGCCT	1 cut(s)	60
Eco47I	GGWCC	2 cut(s)	1477, 2329
Eco57I	CTGAAG	1 cut(s)	947
Eco81I	CCTNAGG	1 cut(s)	583
EcoNI	CCTNNNNNAGG	2 cut(s)	54, 1343
EcoO109I	RGGNCCY	1 cut(s)	1477
EcoRI	GAATTC	2 cut(s)	104, 858
EcoRII	CCWGG	2 cut(s)	49, 471
EcoT14I	CCWWGG	4 cut(s)	91, 400, 1233, 2303
ErhI	CCWWGG	4 cut(s)	91, 400, 1233, 2303
Esp3I	CGTCTC	1 cut(s)	1273
FaeI	CATG	9 cut(s)	70, 1173, 1208, 1373, 1578, 1751, 1786, 1819, 2289
FalI	AAGNNNNNCTT	2 cut(s)	75, 107
FatI	CATG	9 cut(s)	66, 1169, 1204, 1369, 1574, 1747, 1782, 1815, 2285
FauI	CCCGC	1 cut(s)	202
FbaI	TGATCA	1 cut(s)	2374
FblI	GTMKAC	1 cut(s)	1428
Fnu4HI	GCNGC	9 cut(s)	149, 304, 530, 677, 948, 1160, 1661, 1926, 1997
FokI	GGATG	3 cut(s)	1133, 1257, 2468
Fsp4HI	GCNGC	9 cut(s)	149, 304, 530, 677, 948, 1160, 1661, 1926, 1997
FspBI	CTAG	4 cut(s)	188, 251, 971, 1619
GluI	GCNGC	9 cut(s)	149, 304, 530, 677, 948, 1160, 1661, 1926, 1997
GsaI	CCCAGC	1 cut(s)	125
GsuI	CTGGAG	3 cut(s)	206, 1567, 1914
HaeIII	GGCC	5 cut(s)	60, 114, 567, 2075, 2396
HapII	CCGG	3 cut(s)	852, 1546, 2450
Hin1II	CATG	9 cut(s)	70, 1173, 1208, 1373, 1578, 1751, 1786, 1819, 2289
HincII	GTYRAC	1 cut(s)	2455
HindII	GTYRAC	1 cut(s)	2455
HindIII	AAGCTT	1 cut(s)	701
HpaI	GTTAAC	1 cut(s)	2455
HpaII	CCGG	3 cut(s)	852, 1546, 2450
HphI	GGTGA	6 cut(s)	586, 599, 739, 1082, 1844, 2292
Hpy166II	GTNNAC	9 cut(s)	88, 489, 1224, 1429, 1760, 2007, 2164, 2332, 2455
Hpy188I	TCNGA	8 cut(s)	327, 694, 838, 1153, 1392, 1406, 2293, 2523
Hpy8I	GTNNAC	9 cut(s)	88, 489, 1224, 1429, 1760, 2007, 2164, 2332, 2455
Hpy99I	CGWCG	1 cut(s)	2030
HpyAV	CCTTC	6 cut(s)	629, 945, 1349, 1496, 1703, 1958
HpyCH4III	ACNGT	5 cut(s)	1778, 1897, 2089, 2177, 2245
HpyCH4IV	ACGT	1 cut(s)	1461
HpyF10VI	GCNNNNNNNGC	5 cut(s)	405, 772, 885, 1419, 1996
HpyF3I	CTNAG	8 cut(s)	144, 326, 449, 583, 704, 1270, 1535, 2062
HpySE526I	ACGT	1 cut(s)	1461
Hsp92II	CATG	9 cut(s)	70, 1173, 1208, 1373, 1578, 1751, 1786, 1819, 2289
KpnI	GGTACC	2 cut(s)	498, 1079
Ksp22I	TGATCA	1 cut(s)	2374
KspAI	GTTAAC	1 cut(s)	2455
Kzo9I	GATC	8 cut(s)	570, 973, 1035, 1406, 1566, 2104, 2374, 2503
LmnI	GCTCC	2 cut(s)	1586, 1933
Lsp1109I	GCAGC	9 cut(s)	135, 315, 516, 688, 934, 1171, 1647, 1912, 1983
LweI	GCATC	4 cut(s)	1235, 2082, 2347, 2490
MaeI	CTAG	4 cut(s)	188, 251, 971, 1619
MaeII	ACGT	1 cut(s)	1461
MaeIII	GTNAC	4 cut(s)	519, 659, 1778, 2177
MalI	GATC	8 cut(s)	572, 975, 1037, 1408, 1568, 2106, 2376, 2505
MboI	GATC	8 cut(s)	570, 973, 1035, 1406, 1566, 2104, 2374, 2503
MfeI	CAATTG	2 cut(s)	599, 680
MflI	RGATCY	4 cut(s)	570, 973, 1566, 2104
MhlI	GDGCHC	1 cut(s)	1992
MlsI	TGGCCA	2 cut(s)	114, 2075
MluNI	TGGCCA	2 cut(s)	114, 2075
MlyI	GAGTC	3 cut(s)	382, 1845, 1865
MmeI	TCCRAC	1 cut(s)	2288
Mox20I	TGGCCA	2 cut(s)	114, 2075
MroXI	GAANNNNTTC	2 cut(s)	8, 259
MscI	TGGCCA	2 cut(s)	114, 2075
Msp20I	TGGCCA	2 cut(s)	114, 2075
MspA1I	CMGCKG	1 cut(s)	148
MspCI	CTTAAG	1 cut(s)	2270
MspI	CCGG	3 cut(s)	852, 1546, 2450
MspR9I	CCNGG	2 cut(s)	51, 473
MunI	CAATTG	2 cut(s)	599, 680
Mva1269I	GAATGC	3 cut(s)	642, 783, 1529
MvaI	CCWGG	2 cut(s)	51, 473
MwoI	GCNNNNNNNGC	5 cut(s)	405, 772, 885, 1419, 1996
NdeII	GATC	8 cut(s)	570, 973, 1035, 1406, 1566, 2104, 2374, 2503
NlaIII	CATG	9 cut(s)	70, 1173, 1208, 1373, 1578, 1751, 1786, 1819, 2289
NlaIV	GGNNCC	4 cut(s)	496, 914, 1077, 1912
NmeAIII	GCCGAG	1 cut(s)	1815
NmuCI	GTSAC	1 cut(s)	1778
NspI	RCATGY	5 cut(s)	70, 1173, 1208, 1578, 2289
PaeI	GCATGC	3 cut(s)	1173, 1208, 2289
PagI	TCATGA	1 cut(s)	1747
PaqCI	CACCTGC	1 cut(s)	2149
PceI	AGGCCT	1 cut(s)	60
PciI	ACATGT	1 cut(s)	1574
PctI	GAATGC	3 cut(s)	642, 783, 1529
PdmI	GAANNNNTTC	2 cut(s)	8, 259
PfeI	GAWTC	7 cut(s)	427, 548, 927, 1383, 1555, 2297, 2446
PflMI	CCANNNNNTGG	1 cut(s)	656
PkrI	GCNGC	9 cut(s)	150, 305, 531, 678, 949, 1161, 1662, 1927, 1998
PleI	GAGTC	3 cut(s)	382, 1845, 1865
PpsI	GAGTC	3 cut(s)	382, 1845, 1865
PpuMI	RGGWCCY	1 cut(s)	1477
PscI	ACATGT	1 cut(s)	1574
PshBI	ATTAAT	2 cut(s)	1313, 1332
PsiI	TTATAA	2 cut(s)	284, 1596
Psp1406I	AACGTT	1 cut(s)	1461
Psp5II	RGGWCCY	1 cut(s)	1477
Psp6I	CCWGG	2 cut(s)	49, 471
PspFI	CCCAGC	1 cut(s)	121
PspGI	CCWGG	2 cut(s)	49, 471
PspN4I	GGNNCC	4 cut(s)	496, 914, 1077, 1912
PspPI	GGNCC	2 cut(s)	1477, 2329
PspPPI	RGGWCCY	1 cut(s)	1477
PstI	CTGCAG	2 cut(s)	153, 678
PsuI	RGATCY	4 cut(s)	570, 973, 1566, 2104
PvuII	CAGCTG	1 cut(s)	148
RsaI	GTAC	8 cut(s)	89, 97, 156, 496, 933, 1077, 1108, 1185
RsaNI	GTAC	8 cut(s)	88, 96, 155, 495, 932, 1076, 1107, 1184
SatI	GCNGC	9 cut(s)	149, 304, 530, 677, 948, 1160, 1661, 1926, 1997
Sau3AI	GATC	8 cut(s)	570, 973, 1035, 1406, 1566, 2104, 2374, 2503
Sau96I	GGNCC	2 cut(s)	1477, 2329
SchI	GAGTC	3 cut(s)	382, 1845, 1865
ScrFI	CCNGG	2 cut(s)	51, 473
SduI	GDGCHC	1 cut(s)	1992
SfaNI	GCATC	4 cut(s)	1235, 2082, 2347, 2490
SfcI	CTRYAG	5 cut(s)	149, 363, 674, 1016, 2413
SinI	GGWCC	2 cut(s)	1477, 2329
SmlI	CTYRAG	3 cut(s)	1064, 2270, 2458
SmoI	CTYRAG	3 cut(s)	1064, 2270, 2458
SphI	GCATGC	3 cut(s)	1173, 1208, 2289
SseBI	AGGCCT	1 cut(s)	60
SsiI	CCGC	4 cut(s)	7, 195, 1380, 1413
SspI	AATATT	2 cut(s)	655, 1711
SspMI	CTAG	4 cut(s)	188, 251, 971, 1619
StuI	AGGCCT	1 cut(s)	60
StyD4I	CCNGG	2 cut(s)	49, 471
StyI	CCWWGG	4 cut(s)	91, 400, 1233, 2303
TaaI	ACNGT	5 cut(s)	1778, 1897, 2089, 2177, 2245
TaiI	ACGT	1 cut(s)	1464
TaqI	TCGA	4 cut(s)	237, 1328, 2025, 2502
TatI	WGTACW	1 cut(s)	1106
TfiI	GAWTC	7 cut(s)	427, 548, 927, 1383, 1555, 2297, 2446
TscAI	CASTG	7 cut(s)	831, 1539, 1781, 2094, 2248, 2371, 2428
TseFI	GTSAC	1 cut(s)	1778
TseI	GCWGC	9 cut(s)	148, 303, 529, 676, 947, 1159, 1660, 1925, 1996
Tsp45I	GTSAC	1 cut(s)	1778
TspGWI	ACGGA	2 cut(s)	1507, 1883
TspRI	CASTG	7 cut(s)	831, 1539, 1781, 2094, 2248, 2371, 2428
Van91I	CCANNNNNTGG	1 cut(s)	656
Vha464I	CTTAAG	1 cut(s)	2270
VpaK11BI	GGWCC	2 cut(s)	1477, 2329
VspI	ATTAAT	2 cut(s)	1313, 1332
XagI	CCTNNNNNAGG	2 cut(s)	54, 1343
XapI	RAATTY	8 cut(s)	29, 104, 208, 858, 893, 1645, 2224, 2517
XbaI	TCTAGA	1 cut(s)	970
XceI	RCATGY	5 cut(s)	70, 1173, 1208, 1578, 2289
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1513
XmiI	GTMKAC	1 cut(s)	1428
XmnI	GAANNNNTTC	2 cut(s)	8, 259
XspI	CTAG	4 cut(s)	188, 251, 971, 1619

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.