RchiOBHm_Chr6g0248051
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0000248
Physical Location & Seq
Forward (+)
4069013 .. 4073434
4422 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ22237

Sequence Viewer

Length: 3171 bp
ATGGAGCTTCATATGCTCAACTTCTGTGCATTTCGGTCTACCTACCTTCATGCCATGACCACCCTTTTCCTTCTCACCAACCTTTTTCAACCTACCATCTTTGCAAATGCATTGAGCAATGAAACTGATCGCTTGGCTTTGCTGAAATTCAAAGATTGCATAGCCTCTGATCCACATGGGCTGTTGAACTCATGGAATGACTCCGTTCATCACTGCAAATGGCCTGGAATTACTTGTGGCAGACGGCATCAAAGAGTAACAGCCTTGAACACACGAAACGCTGTTTTGCACGGAACCATATCTCCTTACATTGGCAACCTCTCCTTTCTTAGGTTCATCACCCTTCGAAACAACAGCTTCTCTGGCAAGATTCCGCAACAAGTTGAACATTTATTCCGACTCCGACATCTCAATCTCAGTCACAACATGTTGGAGGGGGGAATTCCAGTCAACCTGACCTTGTGTGCGGAACTGAGCATCTTAAGTATTGGAGAAAACCGCCTTACCGGAAAAATTCCTTCAGAGATTGGCTCATTGATGAAGCTTGTGTTTCTCAATCTAGAGATAAACAATCTGACGGGATCCATCCCACCTTCCCTGGGGAATCTTTCATCACTTACTATAGTTTCCTTGGCAATGAACAATTTGGTGGGCACCGTTCCAGAGGTGCTAGGCCGATTGAGAAGCTTATCAATCTTTGCAATTGGTATCAATACTCTATCTGGTATGATCCCTCCCTCCCTTTTTAACATATCATCTATAAATATCGTCTCAATTGAGGATAATAAGTTTAAGGGCAGTATTCCACCTGGCATAGGCCTAAACATGCCTAATCTCCAAATAATGTTCCTCGCCGGAAATGAATTCTCTGGACAAATTCCAGCTTCACTTTCCAATGCTTCTCAGCTTCTTGGGCTTGATTTTGCGGGAAATAATTTTGTTGGCCAACTTCCTGCTAGTTTTGGAAATTTTCCTAATCTCCAGGTGCTCAACTTCGAGTTCAATAATCTAGGAACTAATTCATCAAATGATTTGGGATTTATAACATTCTTGACAAATTACAGCAAACTGGAGGTGTTTTCTCTGAACACTAACAACTTTGGAGGTGTTTTACCCAACTCTGTAGCCAATTTCTCAACCCAACTGACTCAACTCTATCTTGGGGACAATCAAATAGCGGGAACGATTCCTGAAACATTAGGAAATCTTAACAATTTAATACACTTGAGCCTGGACGTAAACTTGTTCACAGGTATCATACCAGCTTCTTTCGGGAAGTTACAAAAACTGCAAAGATTATATTTAAATTCCAATAGATTATCAGGCCGGATCCCATCTTCCTTAGGAAACCTCACCCAATTGTTTGAACTCCGCTTATCAGAAAATGAATTAGAAGGAAGCATTCCTCCAAAGATTGGTAACTGCAAAAATCTGCACCTGATGGATATATCACACAATAAGCTTTGTGGAGATATACCATCACAGGTCATTGGTCTGTCCTCCTTCTCTATCTTGCTCAACTTATCGCAAAACTCGCTAACTGGCATTCTGCCTGTGGAAGTGGGTAAGCTGAAGAATATCAATACACTGGACATCTCTAATAATAATTTGACCGGAGGAATTCCAGAAATTATTGGAGGCTGTCTGAGCCTTGAATTTCTTGACCTACAAGGGAACCACTTTCAAGGAATCATACCTTCTTCTTTGGCTGCTTTGAGAGGTCTTCAGTATCTAGATCTTTCACGAAACAACTTGTCAGGACATATTCCAAAAGACCTACAGAGGCTTCAATTCTTGATCCATTTGAACCTTTCGTTCAATAATCTGGAGGGTGAGGTACCGAGAGAAAGAGTTTTTCGAAACACAAGTGCAATATCATTCGATGGAAATGCCAAACTTTGTGGAGGTGTTTCAGAATTGCAGCTACTAGCATGCCCCATCAAAGTACCAAAGCATAGAAAGTTGCATGGTTTCAAACTAAAGTTCACAGTTTCCTTGGTCGCTGGATGCTCTCTTCTGTTTGCAGTGGTCTTAGCTCTTTATTGGAGGAGAAAAACTCAAAAGACGAAACCATTAATAGCAGTGTCATCAATTAACTTCCTTCCAAAGGTTTCATACCAGACACTTCATCATGCTACTGGCGGATTCTCTCCGAGCAATCAAATTGGATCAGGCGGTTTTGGCTCTGTATACAAAGGGATTCTTAATCAAGAAGAAAACAACGTTGTTGCCATCAAGGTCCTCAACCTTCAACGGAAAGGTGCTTCCAAAAGTTTTGTGGCTGAATGCAATGCACTGAGAAATATCCGGCACAGGAATCTTGTGAAGATCTTAACATGTTGCTCCAGCACGGATTACAATGGTAATGACTTCAAAGCTCTAGTTTTTGAGTATATGTCAAATGGAAGTTTAGAGGAGTGGCTGCACAGAGAAAATCAATCAAGGGGTTTGAACCTTCTTCAAAGACTGAATATTGTTATTGATGTGGCTTCTGCATTGTGTTATCTTCATGACTATTGTGAACCACAAATCATTCACTGCGACATGAAGCCGAGCAACATTCTTCTTGATGATGACATGGTTGCTCGTGTTGGTGATTTTGGGTCAGCAAGACTCTTCTCAACGACCACGGACTCCTCTCAAAATCAAAGTAGCACAGTTGGAATAAAGGGAACCATTGGCTATGCTGCTCCAGAGTATGCGAGTGGTGTTGAGCCATCAAGACAAGGGGATGTATATAGTTATGGGGTGCTCGTGTTGCAAATGTTCACAGGAAGAAGACCTATCGATGAAATGTTTAAAGAGGGTTTGAACCTCCATAACTTTGTCAAGATGGCCATACCAGGAAGAGTGATGCAGATTGTAGATCCTACTCTTCTTGCCACTTTGGAAGACACAGCACCTGCAACATCACAAAATGTAGTGAACTACATCAATGGTTACAATAATGAAATCGAAGCAGTTGAAGAAAACATTGACAATGAGAATTTAAGCAAGATGAACACTTATGTGTGGAAGTGCATACTTCCAACCCTTAAGATTGGACTTGGATGCTCGGAAGAATCACCATGGAATAGAATGGCTATGGAGGAGGTCCACAGCAAGCTACACCATATAAAAGATGCTTACACTGGTGTTGACATTTGTCAAGAAAGGCCAAGAAGAAGCTGA

Protein Analysis

1056

Amino Acids

116.24

Weight (kDa)

8.14

Isoelectric Point (pI)

35.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 41 - 79 8.6e-11 Leucine rich repeat N-terminal domain
LRR_14 PF23598 102 - 333 1.5e-11 Leucine-rich repeat region
LRR_14 PF23598 351 - 460 9.4e-07 Leucine-rich repeat region
LRR_8 PF13855 381 - 440 9.6e-10 Leucine rich repeat
LRR_14 PF23598 507 - 610 7.7e-07 Leucine-rich repeat region
LRR_8 PF13855 551 - 609 1.3e-07 Leucine rich repeat
Pkinase PF00069 719 - 936 2e-41 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 720 - 934 5.1e-39 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000248)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g31390 FvH4_5g31630 FvH4_5g31631 FvH4_7g24151 FvH4_7g24160 FvH4_7g24240 FvH4_7g24242 FvH4_7g24243 FvH4_7g24510 FvH4_7g24920 FvH4_7g24920 FvH4_7g24920 FvH4_7g29910
malus_domestica MD01G1131800.v1.1 MD01G1150000.v1.1 MD01G1151300.v1.1
prunus_persica Prupe.2G248300_v2.0.a1
pyrus_communis pycom01g16800
rosa_chinensis RchiOBHm_Chr1g0339821 RchiOBHm_Chr1g0345151 RchiOBHm_Chr1g0346851 RchiOBHm_Chr1g0351981 RchiOBHm_Chr1g0369731 RchiOBHm_Chr1g0369841 RchiOBHm_Chr1g0369861 RchiOBHm_Chr1g0369931 RchiOBHm_Chr1g0369961 RchiOBHm_Chr1g0370261 RchiOBHm_Chr1g0370281 RchiOBHm_Chr5g0076391 RchiOBHm_Chr6g0248051 RchiOBHm_Chr6g0248071
rosa_laevigata RLG00000002548 RLG00000013479 RLG00000027042 RLG00000027045 RLG00000027047 RLG00000027060 RLG00000027063 RLG00000027082 RLG00000027084 RLG00000027087 RLG00000027089 RLG00000027090 RLG00000027091 RLG00000027092 RLG00000027094 RLG00000027097 RLG00000027100 RLG00000027101 RLG00000027111 RLG00000028769 RLG00000028851 RLG00000029244 RLG00000030021
rosa_multiflora Rmu_co7968444.1_g000001 Rmu_co8060564.1_g000001 Rmu_co8098766.1_g000001 Rmu_co8172886.1_g000001 Rmu_co8291893.1_g000001 Rmu_co8327887.1_g000001 Rmu_co8406981.1_g000001 Rmu_co8449189.1_g000001 Rmu_co8470871.1_g000001 Rmu_sc0000756.1_g000006 Rmu_sc0001154.1_g000028 Rmu_sc0001565.1_g000055 Rmu_sc0001657.1_g000002 Rmu_sc0002095.1_g000014 Rmu_sc0002147.1_g000001 Rmu_sc0002705.1_g000004 Rmu_sc0002705.1_g000005 Rmu_sc0004087.1_g000025 Rmu_sc0005500.1_g000022 Rmu_sc0006707.1_g000005 Rmu_sc0006707.1_g000009 Rmu_sc0008140.1_g000012 Rmu_sc0008140.1_g000019 Rmu_sc0016176.1_g000001 Rmu_sc0016906.1_g000006 Rmu_sc0028980.1_g000001 Rmu_sc0041369.1_g000001
rosa_roxburghii Rroxscaffold_3G00232610 Rroxscaffold_4G00286690 Rroxscaffold_4G00286920 Rroxscaffold_4G00286930 Rroxscaffold_4G00286960 Rroxscaffold_4G00286980 Rroxscaffold_4G00287030 Rroxscaffold_4G00287040 Rroxscaffold_4G00287070 Rroxscaffold_4G00287090 Rroxscaffold_4G00287330 Rroxscaffold_4G00287430 Rroxscaffold_4G00303380 Rroxscaffold_4G00303400 Rroxscaffold_4G00303410 Rroxscaffold_4G00308100 Rroxscaffold_4G00308480 Rroxscaffold_4G00313700 Rroxscaffold_4G00322910 Rroxscaffold_7G00190680 Rroxscaffold_7G00193490
rosa_rugosa Rorug01G0032200 Rorug01G0139400.1 Rorug01G0139600.1 Rorug01G0139700.1 Rorug01G0184400 Rorug01G0351900 Rorug01G0351900 Rorug01G0352600 Rorug01G0352600 Rorug01G0352800 Rorug01G0355300 Rorug01G0355400 Rorug01G0356700.1 Rorug01G0356800 Rorug06G0090600 Rorug07G0276100
rosa_samantha Rh1AG201900 Rh1AG361300 Rh1AG365100 Rh6AG204000
rosa_wichuraiana Rw0G015480 Rw1G007000 Rw1G008190 Rw1G012880 Rw1G012990 Rw1G016950 Rw1G031720 Rw1G031800 Rw1G031820 Rw1G032020 Rw1G032180 Rw6G017720 Rw7G035600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1043
AarI CACCTGC 1 cut(s) 2911
Acc36I ACCTGC 1 cut(s) 2911
Acc65I GGTACC 1 cut(s) 1837
AccB1I GGYRCC 2 cut(s) 653, 1837
AccB7I CCANNNNNTGG 1 cut(s) 1415
AccI GTMKAC 2 cut(s) 38, 2190
AciI CCGC 8 cut(s) 374, 467, 499, 926, 1178, 1372, 2142, 2175
AclI AACGTT 1 cut(s) 2223
AclWI GGATC 9 cut(s) 164, 576, 589, 724, 1324, 1337, 1792, 2176, 2861
AcoI YGGCCR 2 cut(s) 943, 2835
AcuI CTGAAG 3 cut(s) 504, 1592, 1709
AfaI GTAC 2 cut(s) 1839, 1947
AfiI CCNNNNNNNGG 6 cut(s) 311, 330, 599, 815, 1415, 2107
AflII CTTAAG 2 cut(s) 481, 3035
AflIII ACRYGT 2 cut(s) 426, 2336
AjnI CCWGG 6 cut(s) 223, 597, 808, 981, 1230, 2842
AleI CACNNNNGTG 1 cut(s) 3008
AloI GAACNNNNNNTCC 4 cut(s) 286, 318, 378, 410
Alw21I GWGCWC 2 cut(s) 990, 2754
Alw26I GTCTC 1 cut(s) 775
AlwI GGATC 9 cut(s) 164, 576, 589, 724, 1324, 1337, 1792, 2176, 2861
AlwNI CAGNNNCTG 1 cut(s) 2903
AoxI GGCC 7 cut(s) 221, 673, 817, 943, 1324, 2835, 3155
ApeKI GCWGC 4 cut(s) 1709, 1921, 2422, 2687
AseI ATTAAT 1 cut(s) 2075
Asp700I GAANNNNTTC 1 cut(s) 1018
Asp718I GGTACC 1 cut(s) 1837
AspS9I GGNCC 2 cut(s) 2239, 3094
AsuHPI GGTGA 6 cut(s) 67, 331, 1345, 1844, 2606, 3057
AsuII TTCGAA 2 cut(s) 346, 1858
AvaII GGWCC 2 cut(s) 2239, 3094
AxyI CCTNAGG 1 cut(s) 1342
BaeGI GKGCMC 1 cut(s) 656
BalI TGGCCA 2 cut(s) 945, 2837
BamHI GGATCC 2 cut(s) 581, 1329
BanI GGYRCC 2 cut(s) 653, 1837
BauI CACGAG 2 cut(s) 2586, 2753
BbsI GAAGAC 3 cut(s) 1715, 2785, 2898
Bbv12I GWGCWC 2 cut(s) 990, 2754
BbvI GCAGC 4 cut(s) 1696, 1933, 2409, 2674
BceAI ACGGC 1 cut(s) 260
BcgI CGANNNNNNTGC 2 cut(s) 1871, 1905
BciT130I CCWGG 6 cut(s) 225, 599, 810, 983, 1232, 2844
BcoDI GTCTC 1 cut(s) 775
BfaI CTAG 7 cut(s) 560, 671, 957, 1010, 1733, 1928, 2381
BfmI CTRYAG 3 cut(s) 621, 1122, 1778
BfrI CTTAAG 2 cut(s) 481, 3035
BfuAI ACCTGC 1 cut(s) 2911
BglII AGATCT 2 cut(s) 1735, 2328
BisI GCNGC 4 cut(s) 1710, 1922, 2423, 2688
BlsI GCNGC 4 cut(s) 1711, 1923, 2424, 2689
Bme1390I CCNGG 6 cut(s) 225, 599, 810, 983, 1232, 2844
Bme18I GGWCC 2 cut(s) 2239, 3094
BmgT120I GGNCC 2 cut(s) 2239, 3094
BmiI GGNNCC 7 cut(s) 295, 583, 655, 1331, 1676, 1839, 2674
BmrFI CCNGG 6 cut(s) 225, 599, 810, 983, 1232, 2844
BmsI GCATC 6 cut(s) 256, 486, 1997, 2844, 3041, 3112
BoxI GACNNNNGTC 1 cut(s) 3144
BpiI GAAGAC 3 cut(s) 1715, 2785, 2898
BplI GAGNNNNNCTC 4 cut(s) 515, 547, 2041, 2073
BpmI CTGGAG 5 cut(s) 965, 1091, 1847, 2329, 2676
Bpu14I TTCGAA 2 cut(s) 346, 1858
BpuEI CTTGAG 1 cut(s) 1246
Bsa29I ATCGAT 1 cut(s) 2787
BsaJI CCNNGG 6 cut(s) 597, 598, 630, 1995, 2628, 3068
BsaWI WCCGGW 2 cut(s) 506, 1613
BsaXI ACNNNNNCTCC 2 cut(s) 286, 316
Bsc4I CCNNNNNNNGG 6 cut(s) 311, 330, 599, 815, 1415, 2107
Bse1I ACTGG 6 cut(s) 446, 1074, 1546, 1593, 2143, 3136
Bse21I CCTNAGG 1 cut(s) 1342
Bse3DI GCAATG 3 cut(s) 124, 642, 2296
BseBI CCWGG 6 cut(s) 225, 599, 810, 983, 1232, 2844
BseCI ATCGAT 1 cut(s) 2787
BseDI CCNNGG 6 cut(s) 597, 598, 630, 1995, 2628, 3068
BseGI GGATG 4 cut(s) 585, 2012, 2737, 3056
BseLI CCNNNNNNNGG 6 cut(s) 311, 330, 599, 815, 1415, 2107
BseMI GCAATG 3 cut(s) 124, 642, 2296
BseMII CTCAG 5 cut(s) 430, 464, 917, 1637, 2288
BseNI ACTGG 6 cut(s) 446, 1074, 1546, 1593, 2143, 3136
BseRI GAGGAG 4 cut(s) 2062, 2429, 2626, 3104
BseSI GKGCMC 1 cut(s) 656
BseXI GCAGC 4 cut(s) 1696, 1933, 2409, 2674
BsgI GTGCAG 2 cut(s) 1418, 2408
BshFI GGCC 7 cut(s) 223, 675, 819, 945, 1326, 2837, 3157
BshNI GGYRCC 2 cut(s) 653, 1837
BshVI ATCGAT 1 cut(s) 2787
BsiHKAI GWGCWC 2 cut(s) 990, 2754
BsiSI CCGG 5 cut(s) 507, 855, 1327, 1614, 2308
BslFI GGGAC 1 cut(s) 1178
BslI CCNNNNNNNGG 6 cut(s) 311, 330, 599, 815, 1415, 2107
BsmAI GTCTC 1 cut(s) 775
BsmBI CGTCTC 1 cut(s) 775
BsmFI GGGAC 1 cut(s) 1178
BsmI GAATGC 3 cut(s) 1401, 1545, 2291
BsnI GGCC 7 cut(s) 223, 675, 819, 945, 1326, 2837, 3157
Bsp119I TTCGAA 2 cut(s) 346, 1858
Bsp1286I GDGCHC 3 cut(s) 656, 990, 2754
Bsp19I CCATGG 1 cut(s) 3068
BspACI CCGC 8 cut(s) 374, 467, 499, 926, 1178, 1372, 2142, 2175
BspANI GGCC 7 cut(s) 223, 675, 819, 945, 1326, 2837, 3157
BspCNI CTCAG 5 cut(s) 429, 465, 916, 1638, 2289
BspDI ATCGAT 1 cut(s) 2787
BspHI TCATGA 1 cut(s) 2509
BspLI GGNNCC 7 cut(s) 295, 583, 655, 1331, 1676, 1839, 2674
BspMI ACCTGC 1 cut(s) 2911
BspPI GGATC 9 cut(s) 164, 576, 589, 724, 1324, 1337, 1792, 2176, 2861
BspT104I TTCGAA 2 cut(s) 346, 1858
BspT107I GGYRCC 2 cut(s) 653, 1837
BspTI CTTAAG 2 cut(s) 481, 3035
BsrDI GCAATG 3 cut(s) 124, 642, 2296
BsrI ACTGG 6 cut(s) 446, 1074, 1546, 1593, 2143, 3136
BssECI CCNNGG 6 cut(s) 597, 598, 630, 1995, 2628, 3068
BssNAI GTATAC 1 cut(s) 2191
BssSI CACGAG 2 cut(s) 2586, 2753
BssT1I CCWWGG 3 cut(s) 630, 1995, 3068
Bst1107I GTATAC 1 cut(s) 2191
Bst2BI CACGAG 2 cut(s) 2586, 2753
Bst2UI CCWGG 6 cut(s) 225, 599, 810, 983, 1232, 2844
Bst4CI ACNGT 3 cut(s) 658, 1990, 2659
Bst6I CTCTTC 4 cut(s) 2019, 2621, 2842, 2880
BstAFI CTTAAG 2 cut(s) 481, 3035
BstBI TTCGAA 2 cut(s) 346, 1858
BstC8I GCNNGC 2 cut(s) 1933, 3104
BstDEI CTNAG 8 cut(s) 329, 416, 473, 903, 1342, 1646, 2032, 2297
BstDSI CCRYGG 2 cut(s) 2628, 3068
BstENI CCTNNNNNAGG 3 cut(s) 328, 813, 2105
BstF5I GGATG 4 cut(s) 585, 2012, 2737, 3056
BstMAI GTCTC 1 cut(s) 775
BstMWI GCNNNNNNNGC 7 cut(s) 13, 363, 913, 1534, 1647, 2181, 2758
BstNI CCWGG 6 cut(s) 225, 599, 810, 983, 1232, 2844
BstNSI RCATGY 4 cut(s) 430, 829, 1935, 2340
BstPAI GACNNNNGTC 1 cut(s) 3144
BstSCI CCNGG 6 cut(s) 223, 597, 808, 981, 1230, 2842
BstSFI CTRYAG 3 cut(s) 621, 1122, 1778
BstSLI GKGCMC 1 cut(s) 656
BstV1I GCAGC 4 cut(s) 1696, 1933, 2409, 2674
BstV2I GAAGAC 3 cut(s) 1715, 2785, 2898
BstX2I RGATCY 5 cut(s) 581, 1329, 1735, 2328, 2866
BstYI RGATCY 5 cut(s) 581, 1329, 1735, 2328, 2866
BstZ17I GTATAC 1 cut(s) 2191
Bsu15I ATCGAT 1 cut(s) 2787
Bsu36I CCTNAGG 1 cut(s) 1342
BsuRI GGCC 7 cut(s) 223, 675, 819, 945, 1326, 2837, 3157
BsuTUI ATCGAT 1 cut(s) 2787
BtgI CCRYGG 2 cut(s) 2628, 3068
BtsCI GGATG 4 cut(s) 585, 2012, 2737, 3056
BtsI GCAGTG 4 cut(s) 211, 2031, 2088, 2536
BtsIMutI CAGTG 7 cut(s) 211, 1586, 2031, 2088, 2294, 2536, 3129
BveI ACCTGC 1 cut(s) 2911
Cac8I GCNNGC 2 cut(s) 1933, 3104
CaiI CAGNNNCTG 1 cut(s) 2903
CciI TCATGA 1 cut(s) 2509
Cfr13I GGNCC 2 cut(s) 2239, 3094
ClaI ATCGAT 1 cut(s) 2787
Csp6I GTAC 2 cut(s) 1838, 1946
CspCI CAANNNNNGTGG 2 cut(s) 1882, 1917
CviQI GTAC 2 cut(s) 1838, 1946
DdeI CTNAG 8 cut(s) 329, 416, 473, 903, 1342, 1646, 2032, 2297
DraI TTTAAA 2 cut(s) 1305, 2800
EaeI YGGCCR 2 cut(s) 943, 2835
Eam1104I CTCTTC 4 cut(s) 2019, 2621, 2842, 2880
EarI CTCTTC 4 cut(s) 2019, 2621, 2842, 2880
EciI GGCGGA 1 cut(s) 2157
Eco130I CCWWGG 3 cut(s) 630, 1995, 3068
Eco147I AGGCCT 1 cut(s) 819
Eco47I GGWCC 2 cut(s) 2239, 3094
Eco57I CTGAAG 3 cut(s) 504, 1592, 1709
Eco81I CCTNAGG 1 cut(s) 1342
EcoNI CCTNNNNNAGG 3 cut(s) 328, 813, 2105
EcoO109I RGGNCCY 1 cut(s) 2239
EcoRI GAATTC 3 cut(s) 441, 863, 1620
EcoRII CCWGG 6 cut(s) 223, 597, 808, 981, 1230, 2842
EcoT14I CCWWGG 3 cut(s) 630, 1995, 3068
EcoT22I ATGCAT 1 cut(s) 112
ErhI CCWWGG 3 cut(s) 630, 1995, 3068
Esp3I CGTCTC 1 cut(s) 775
FalI AAGNNNNNCTT 2 cut(s) 2187, 2219
FaqI GGGAC 1 cut(s) 1178
FauI CCCGC 2 cut(s) 919, 1171
FauNDI CATATG 1 cut(s) 12
FblI GTMKAC 2 cut(s) 38, 2190
Fnu4HI GCNGC 4 cut(s) 1710, 1922, 2423, 2688
FokI GGATG 4 cut(s) 572, 2019, 2744, 3063
Fsp4HI GCNGC 4 cut(s) 1710, 1922, 2423, 2688
FspBI CTAG 7 cut(s) 560, 671, 957, 1010, 1733, 1928, 2381
GluI GCNGC 4 cut(s) 1710, 1922, 2423, 2688
GsuI CTGGAG 5 cut(s) 965, 1091, 1847, 2329, 2676
HaeIII GGCC 7 cut(s) 223, 675, 819, 945, 1326, 2837, 3157
HapII CCGG 5 cut(s) 507, 855, 1327, 1614, 2308
HincII GTYRAC 2 cut(s) 451, 3139
HindII GTYRAC 2 cut(s) 451, 3139
HindIII AAGCTT 3 cut(s) 542, 685, 1460
HpaII CCGG 5 cut(s) 507, 855, 1327, 1614, 2308
HphI GGTGA 6 cut(s) 67, 331, 1345, 1844, 2606, 3057
HpyCH4III ACNGT 3 cut(s) 658, 1990, 2659
HpyCH4IV ACGT 2 cut(s) 1236, 2223
HpyF10VI GCNNNNNNNGC 7 cut(s) 13, 363, 913, 1534, 1647, 2181, 2758
HpyF3I CTNAG 8 cut(s) 329, 416, 473, 903, 1342, 1646, 2032, 2297
HpySE526I ACGT 2 cut(s) 1236, 2223
KpnI GGTACC 1 cut(s) 1841
LmnI GCTCC 3 cut(s) 4, 2348, 2695
Lsp1109I GCAGC 4 cut(s) 1696, 1933, 2409, 2674
LweI GCATC 6 cut(s) 256, 486, 1997, 2844, 3041, 3112
MaeI CTAG 7 cut(s) 560, 671, 957, 1010, 1733, 1928, 2381
MaeII ACGT 2 cut(s) 1236, 2223
MaeIII GTNAC 5 cut(s) 256, 419, 1278, 1418, 2939
MfeI CAATTG 3 cut(s) 702, 774, 1358
MflI RGATCY 5 cut(s) 581, 1329, 1735, 2328, 2866
MhlI GDGCHC 3 cut(s) 656, 990, 2754
MlsI TGGCCA 2 cut(s) 945, 2837
MluNI TGGCCA 2 cut(s) 945, 2837
MlyI GAGTC 5 cut(s) 194, 393, 1141, 2607, 2627
MmeI TCCRAC 5 cut(s) 411, 421, 427, 2641, 3053
Mox20I TGGCCA 2 cut(s) 945, 2837
Mph1103I ATGCAT 1 cut(s) 112
MroXI GAANNNNTTC 1 cut(s) 1018
MscI TGGCCA 2 cut(s) 945, 2837
MslI CAYNNNNRTG 1 cut(s) 3008
Msp20I TGGCCA 2 cut(s) 945, 2837
MspCI CTTAAG 2 cut(s) 481, 3035
MspI CCGG 5 cut(s) 507, 855, 1327, 1614, 2308
MspR9I CCNGG 6 cut(s) 225, 599, 810, 983, 1232, 2844
MunI CAATTG 3 cut(s) 702, 774, 1358
Mva1269I GAATGC 3 cut(s) 1401, 1545, 2291
MvaI CCWGG 6 cut(s) 225, 599, 810, 983, 1232, 2844
MwoI GCNNNNNNNGC 7 cut(s) 13, 363, 913, 1534, 1647, 2181, 2758
NcoI CCATGG 1 cut(s) 3068
NdeI CATATG 1 cut(s) 12
NlaIV GGNNCC 7 cut(s) 295, 583, 655, 1331, 1676, 1839, 2674
NmeAIII GCCGAG 1 cut(s) 2577
NmuCI GTSAC 1 cut(s) 419
NsiI ATGCAT 1 cut(s) 112
NspI RCATGY 4 cut(s) 430, 829, 1935, 2340
NspV TTCGAA 2 cut(s) 346, 1858
OliI CACNNNNGTG 1 cut(s) 3008
PaeI GCATGC 1 cut(s) 1935
PagI TCATGA 1 cut(s) 2509
PaqCI CACCTGC 1 cut(s) 2911
PasI CCCWGGG 1 cut(s) 598
PceI AGGCCT 1 cut(s) 819
PciI ACATGT 2 cut(s) 426, 2336
PctI GAATGC 3 cut(s) 1401, 1545, 2291
PdmI GAANNNNTTC 1 cut(s) 1018
PfeI GAWTC 8 cut(s) 370, 604, 1186, 1689, 2145, 2200, 2317, 3062
PflMI CCANNNNNTGG 1 cut(s) 1415
PkrI GCNGC 4 cut(s) 1711, 1923, 2424, 2689
PleI GAGTC 5 cut(s) 194, 393, 1141, 2607, 2627
PpsI GAGTC 5 cut(s) 194, 393, 1141, 2607, 2627
PpuMI RGGWCCY 1 cut(s) 2239
PscI ACATGT 2 cut(s) 426, 2336
PshAI GACNNNNGTC 1 cut(s) 3144
PshBI ATTAAT 1 cut(s) 2075
PsiI TTATAA 1 cut(s) 1043
Psp1406I AACGTT 1 cut(s) 2223
Psp5II RGGWCCY 1 cut(s) 2239
Psp6I CCWGG 6 cut(s) 223, 597, 808, 981, 1230, 2842
PspGI CCWGG 6 cut(s) 223, 597, 808, 981, 1230, 2842
PspN4I GGNNCC 7 cut(s) 295, 583, 655, 1331, 1676, 1839, 2674
PspPI GGNCC 2 cut(s) 2239, 3094
PspPPI RGGWCCY 1 cut(s) 2239
PstNI CAGNNNCTG 1 cut(s) 2903
PsuI RGATCY 5 cut(s) 581, 1329, 1735, 2328, 2866
RsaI GTAC 2 cut(s) 1839, 1947
RsaNI GTAC 2 cut(s) 1838, 1946
RseI CAYNNNNRTG 1 cut(s) 3008
SatI GCNGC 4 cut(s) 1710, 1922, 2423, 2688
Sau96I GGNCC 2 cut(s) 2239, 3094
SchI GAGTC 5 cut(s) 194, 393, 1141, 2607, 2627
ScrFI CCNGG 6 cut(s) 225, 599, 810, 983, 1232, 2844
SduI GDGCHC 3 cut(s) 656, 990, 2754
SfaNI GCATC 6 cut(s) 256, 486, 1997, 2844, 3041, 3112
SfcI CTRYAG 3 cut(s) 621, 1122, 1778
SfuI TTCGAA 2 cut(s) 346, 1858
SinI GGWCC 2 cut(s) 2239, 3094
SmiI ATTTAAAT 1 cut(s) 1305
SmiMI CAYNNNNRTG 1 cut(s) 3008
SmlI CTYRAG 3 cut(s) 481, 1225, 3035
SmoI CTYRAG 3 cut(s) 481, 1225, 3035
SphI GCATGC 1 cut(s) 1935
SseBI AGGCCT 1 cut(s) 819
SsiI CCGC 8 cut(s) 374, 467, 499, 926, 1178, 1372, 2142, 2175
SspI AATATT 1 cut(s) 2473
SspMI CTAG 7 cut(s) 560, 671, 957, 1010, 1733, 1928, 2381
StuI AGGCCT 1 cut(s) 819
StyD4I CCNGG 6 cut(s) 223, 597, 808, 981, 1230, 2842
StyI CCWWGG 3 cut(s) 630, 1995, 3068
SwaI ATTTAAAT 1 cut(s) 1305
TaaI ACNGT 3 cut(s) 658, 1990, 2659
TaiI ACGT 2 cut(s) 1239, 2226
TaqI TCGA 6 cut(s) 346, 996, 1858, 1881, 2787, 2955
TaqII GACCGA 1 cut(s) 24
TfiI GAWTC 8 cut(s) 370, 604, 1186, 1689, 2145, 2200, 2317, 3062
TscAI CASTG 7 cut(s) 218, 1593, 2031, 2088, 2301, 2543, 3136
TseFI GTSAC 1 cut(s) 419
TseI GCWGC 4 cut(s) 1709, 1921, 2422, 2687
Tsp45I GTSAC 1 cut(s) 419
TspGWI ACGGA 5 cut(s) 193, 306, 2269, 2366, 2645
TspRI CASTG 7 cut(s) 218, 1593, 2031, 2088, 2301, 2543, 3136
Van91I CCANNNNNTGG 1 cut(s) 1415
Vha464I CTTAAG 2 cut(s) 481, 3035
VpaK11BI GGWCC 2 cut(s) 2239, 3094
VspI ATTAAT 1 cut(s) 2075
XagI CCTNNNNNAGG 3 cut(s) 328, 813, 2105
XbaI TCTAGA 2 cut(s) 559, 1732
XceI RCATGY 4 cut(s) 430, 829, 1935, 2340
XcmI CCANNNNNNNNNTGG 1 cut(s) 2275
XmiI GTMKAC 2 cut(s) 38, 2190
XmnI GAANNNNTTC 1 cut(s) 1018
XspI CTAG 7 cut(s) 560, 671, 957, 1010, 1733, 1928, 2381
Zsp2I ATGCAT 1 cut(s) 112
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.