Belongs to the protein kinase superfamily. Ser Thr protein kinase family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 2 cut(s) | 482, 1043 |
| Acc36I | ACCTGC | 1 cut(s) | 279 |
| AccI | GTMKAC | 1 cut(s) | 38 |
| AccIII | TCCGGA | 1 cut(s) | 778 |
| AciI | CCGC | 2 cut(s) | 374, 806 |
| AclI | AACGTT | 1 cut(s) | 1046 |
| AclWI | GGATC | 2 cut(s) | 164, 724 |
| AcoI | YGGCCR | 1 cut(s) | 943 |
| AcsI | RAATTY | 6 cut(s) | 146, 513, 863, 967, 1257, 1292 |
| AcuI | CTGAAG | 4 cut(s) | 504, 893, 1229, 1346 |
| AfaI | GTAC | 1 cut(s) | 848 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 311, 815 |
| AflIII | ACRYGT | 1 cut(s) | 426 |
| AgsI | TTSAA | 8 cut(s) | 151, 187, 268, 386, 424, 793, 1292, 1322 |
| AjnI | CCWGG | 1 cut(s) | 808 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 378, 410 |
| Alw26I | GTCTC | 1 cut(s) | 775 |
| AlwI | GGATC | 2 cut(s) | 164, 724 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 281 |
| Aor13HI | TCCGGA | 1 cut(s) | 778 |
| AoxI | GGCC | 3 cut(s) | 179, 817, 943 |
| ApeKI | GCWGC | 2 cut(s) | 993, 1062 |
| ApoI | RAATTY | 6 cut(s) | 146, 513, 863, 967, 1257, 1292 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 1018 |
| AspS9I | GGNCC | 3 cut(s) | 179, 581, 707 |
| AsuHPI | GGTGA | 3 cut(s) | 67, 448, 1087 |
| AvaII | GGWCC | 2 cut(s) | 581, 707 |
| AxyI | CCTNAGG | 1 cut(s) | 631 |
| BalI | TGGCCA | 1 cut(s) | 945 |
| BbsI | GAAGAC | 1 cut(s) | 1352 |
| BbvI | GCAGC | 2 cut(s) | 980, 1074 |
| BccI | CCATC | 6 cut(s) | 170, 486, 593, 598, 1072, 1123 |
| BceAI | ACGGC | 1 cut(s) | 166 |
| BciT130I | CCWGG | 1 cut(s) | 810 |
| BcoDI | GTCTC | 1 cut(s) | 775 |
| BfaI | CTAG | 3 cut(s) | 416, 1010, 1370 |
| BfuAI | ACCTGC | 1 cut(s) | 279 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 507 |
| BglII | AGATCT | 2 cut(s) | 406, 1372 |
| BisI | GCNGC | 2 cut(s) | 994, 1063 |
| BlpI | GCTNAGC | 1 cut(s) | 989 |
| BlsI | GCNGC | 2 cut(s) | 995, 1064 |
| Bme1390I | CCNGG | 1 cut(s) | 810 |
| Bme18I | GGWCC | 2 cut(s) | 581, 707 |
| BmgT120I | GGNCC | 3 cut(s) | 179, 581, 707 |
| BmiI | GGNNCC | 5 cut(s) | 295, 582, 583, 709, 1313 |
| BmrFI | CCNGG | 1 cut(s) | 810 |
| BpiI | GAAGAC | 1 cut(s) | 1352 |
| BpmI | CTGGAG | 3 cut(s) | 965, 1091, 1272 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 989 |
| Bsa29I | ATCGAT | 1 cut(s) | 130 |
| BsaBI | GATNNNNATC | 1 cut(s) | 411 |
| BsaJI | CCNNGG | 2 cut(s) | 597, 850 |
| BsaWI | WCCGGW | 1 cut(s) | 778 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 311, 815 |
| Bse118I | RCCGGY | 1 cut(s) | 506 |
| Bse1I | ACTGG | 6 cut(s) | 446, 982, 1074, 1183, 1230, 1255 |
| Bse21I | CCTNAGG | 1 cut(s) | 631 |
| Bse3DI | GCAATG | 2 cut(s) | 124, 159 |
| Bse8I | GATNNNNATC | 1 cut(s) | 411 |
| BseAI | TCCGGA | 1 cut(s) | 778 |
| BseBI | CCWGG | 1 cut(s) | 810 |
| BseCI | ATCGAT | 1 cut(s) | 130 |
| BseDI | CCNNGG | 2 cut(s) | 597, 850 |
| BseGI | GGATG | 2 cut(s) | 497, 585 |
| BseJI | GATNNNNATC | 1 cut(s) | 411 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 311, 815 |
| BseMI | GCAATG | 2 cut(s) | 124, 159 |
| BseMII | CTCAG | 2 cut(s) | 464, 1003 |
| BseNI | ACTGG | 6 cut(s) | 446, 982, 1074, 1183, 1230, 1255 |
| BseRI | GAGGAG | 2 cut(s) | 680, 1129 |
| BseXI | GCAGC | 2 cut(s) | 980, 1074 |
| BseYI | CCCAGC | 1 cut(s) | 880 |
| BsgI | GTGCAG | 1 cut(s) | 606 |
| BshFI | GGCC | 3 cut(s) | 181, 819, 945 |
| BshVI | ATCGAT | 1 cut(s) | 130 |
| BsiSI | CCGG | 2 cut(s) | 507, 779 |
| BslFI | GGGAC | 2 cut(s) | 594, 693 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 311, 815 |
| BsmAI | GTCTC | 1 cut(s) | 775 |
| BsmBI | CGTCTC | 1 cut(s) | 775 |
| BsmFI | GGGAC | 2 cut(s) | 594, 693 |
| BsmI | GAATGC | 2 cut(s) | 441, 1182 |
| BsnI | GGCC | 3 cut(s) | 181, 819, 945 |
| Bsp13I | TCCGGA | 1 cut(s) | 778 |
| Bsp143I | GATC | 5 cut(s) | 127, 169, 406, 729, 1372 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 989 |
| BspACI | CCGC | 2 cut(s) | 374, 806 |
| BspANI | GGCC | 3 cut(s) | 181, 819, 945 |
| BspCNI | CTCAG | 2 cut(s) | 465, 1002 |
| BspDI | ATCGAT | 1 cut(s) | 130 |
| BspEI | TCCGGA | 1 cut(s) | 778 |
| BspHI | TCATGA | 1 cut(s) | 54 |
| BspLI | GGNNCC | 5 cut(s) | 295, 582, 583, 709, 1313 |
| BspMI | ACCTGC | 1 cut(s) | 279 |
| BspPI | GGATC | 2 cut(s) | 164, 724 |
| BsrDI | GCAATG | 2 cut(s) | 124, 159 |
| BsrFI | RCCGGY | 1 cut(s) | 506 |
| BsrI | ACTGG | 6 cut(s) | 446, 982, 1074, 1183, 1230, 1255 |
| BssAI | RCCGGY | 1 cut(s) | 506 |
| BssECI | CCNNGG | 2 cut(s) | 597, 850 |
| BssMI | GATC | 5 cut(s) | 127, 169, 406, 729, 1372 |
| BssT1I | CCWWGG | 1 cut(s) | 597 |
| Bst2UI | CCWGG | 1 cut(s) | 810 |
| BstC8I | GCNNGC | 1 cut(s) | 546 |
| BstDEI | CTNAG | 6 cut(s) | 329, 473, 631, 903, 989, 1100 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 813 |
| BstF5I | GGATG | 2 cut(s) | 497, 585 |
| BstKTI | GATC | 5 cut(s) | 130, 172, 409, 732, 1375 |
| BstMAI | GTCTC | 1 cut(s) | 775 |
| BstMBI | GATC | 5 cut(s) | 127, 169, 406, 729, 1372 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 13, 363, 507, 913, 1171 |
| BstNI | CCWGG | 1 cut(s) | 810 |
| BstNSI | RCATGY | 2 cut(s) | 430, 829 |
| BstSCI | CCNGG | 1 cut(s) | 808 |
| BstV1I | GCAGC | 2 cut(s) | 980, 1074 |
| BstV2I | GAAGAC | 1 cut(s) | 1352 |
| BstX2I | RGATCY | 2 cut(s) | 406, 1372 |
| BstYI | RGATCY | 2 cut(s) | 406, 1372 |
| Bsu15I | ATCGAT | 1 cut(s) | 130 |
| Bsu36I | CCTNAGG | 1 cut(s) | 631 |
| BsuRI | GGCC | 3 cut(s) | 181, 819, 945 |
| BsuTUI | ATCGAT | 1 cut(s) | 130 |
| BtsCI | GGATG | 2 cut(s) | 497, 585 |
| BtsI | GCAGTG | 1 cut(s) | 618 |
| BtsIMutI | CAGTG | 1 cut(s) | 618 |
| BveI | ACCTGC | 1 cut(s) | 279 |
| Cac8I | GCNNGC | 1 cut(s) | 546 |
| CaiI | CAGNNNCTG | 1 cut(s) | 281 |
| CciI | TCATGA | 1 cut(s) | 54 |
| Cfr10I | RCCGGY | 1 cut(s) | 506 |
| Cfr13I | GGNCC | 3 cut(s) | 179, 581, 707 |
| ClaI | ATCGAT | 1 cut(s) | 130 |
| CseI | GACGC | 1 cut(s) | 287 |
| Csp6I | GTAC | 1 cut(s) | 847 |
| CviAII | CATG | 6 cut(s) | 50, 55, 192, 290, 427, 826 |
| CviQI | GTAC | 1 cut(s) | 847 |
| DdeI | CTNAG | 6 cut(s) | 329, 473, 631, 903, 989, 1100 |
| DpnI | GATC | 5 cut(s) | 129, 171, 408, 731, 1374 |
| DpnII | GATC | 5 cut(s) | 127, 169, 406, 729, 1372 |
| EaeI | YGGCCR | 1 cut(s) | 943 |
| EciI | GGCGGA | 1 cut(s) | 795 |
| Eco130I | CCWWGG | 1 cut(s) | 597 |
| Eco147I | AGGCCT | 1 cut(s) | 819 |
| Eco47I | GGWCC | 2 cut(s) | 581, 707 |
| Eco57I | CTGAAG | 4 cut(s) | 504, 893, 1229, 1346 |
| Eco81I | CCTNAGG | 1 cut(s) | 631 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 813 |
| EcoO109I | RGGNCCY | 1 cut(s) | 581 |
| EcoRI | GAATTC | 2 cut(s) | 863, 1257 |
| EcoRII | CCWGG | 1 cut(s) | 808 |
| EcoT14I | CCWWGG | 1 cut(s) | 597 |
| EcoT22I | ATGCAT | 1 cut(s) | 112 |
| ErhI | CCWWGG | 1 cut(s) | 597 |
| Esp3I | CGTCTC | 1 cut(s) | 775 |
| FaeI | CATG | 6 cut(s) | 53, 58, 195, 293, 430, 829 |
| FaqI | GGGAC | 2 cut(s) | 594, 693 |
| FatI | CATG | 6 cut(s) | 49, 54, 191, 289, 426, 825 |
| FauNDI | CATATG | 1 cut(s) | 12 |
| FblI | GTMKAC | 1 cut(s) | 38 |
| Fnu4HI | GCNGC | 2 cut(s) | 994, 1063 |
| FokI | GGATG | 2 cut(s) | 504, 572 |
| Fsp4HI | GCNGC | 2 cut(s) | 994, 1063 |
| FspBI | CTAG | 3 cut(s) | 416, 1010, 1370 |
| GluI | GCNGC | 2 cut(s) | 994, 1063 |
| GsaI | CCCAGC | 1 cut(s) | 884 |
| GsuI | CTGGAG | 3 cut(s) | 965, 1091, 1272 |
| HaeIII | GGCC | 3 cut(s) | 181, 819, 945 |
| HapII | CCGG | 2 cut(s) | 507, 779 |
| HgaI | GACGC | 1 cut(s) | 287 |
| Hin1II | CATG | 6 cut(s) | 53, 58, 195, 293, 430, 829 |
| HindIII | AAGCTT | 4 cut(s) | 542, 685, 905, 1097 |
| HinfI | GANTC | 8 cut(s) | 132, 155, 200, 333, 370, 604, 998, 1326 |
| HpaII | CCGG | 2 cut(s) | 507, 779 |
| HphI | GGTGA | 3 cut(s) | 67, 448, 1087 |
| Hpy166II | GTNNAC | 2 cut(s) | 39, 208 |
| Hpy188I | TCNGA | 7 cut(s) | 523, 576, 839, 854, 1237, 1274, 1284 |
| Hpy8I | GTNNAC | 2 cut(s) | 39, 208 |
| Hpy99I | CGWCG | 1 cut(s) | 770 |
| HpyAV | CCTTC | 6 cut(s) | 56, 80, 528, 744, 787, 1344 |
| HpyCH4IV | ACGT | 2 cut(s) | 765, 1046 |
| HpyCH4V | TGCA | 9 cut(s) | 29, 104, 110, 216, 289, 467, 623, 701, 1062 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 13, 363, 507, 913, 1171 |
| HpyF3I | CTNAG | 6 cut(s) | 329, 473, 631, 903, 989, 1100 |
| HpySE526I | ACGT | 2 cut(s) | 765, 1046 |
| Hsp92II | CATG | 6 cut(s) | 53, 58, 195, 293, 430, 829 |
| KflI | GGGWCCC | 1 cut(s) | 581 |
| Kpn2I | TCCGGA | 1 cut(s) | 778 |
| Kzo9I | GATC | 5 cut(s) | 127, 169, 406, 729, 1372 |
| LmnI | GCTCC | 2 cut(s) | 4, 913 |
| Lsp1109I | GCAGC | 2 cut(s) | 980, 1074 |
| MaeI | CTAG | 3 cut(s) | 416, 1010, 1370 |
| MaeII | ACGT | 2 cut(s) | 765, 1046 |
| MaeIII | GTNAC | 2 cut(s) | 256, 1345 |
| MalI | GATC | 5 cut(s) | 129, 171, 408, 731, 1374 |
| MboI | GATC | 5 cut(s) | 127, 169, 406, 729, 1372 |
| MboII | GAAGA | 5 cut(s) | 585, 852, 1222, 1329, 1352 |
| MfeI | CAATTG | 1 cut(s) | 702 |
| MflI | RGATCY | 2 cut(s) | 406, 1372 |
| MlsI | TGGCCA | 1 cut(s) | 945 |
| MluNI | TGGCCA | 1 cut(s) | 945 |
| MlyI | GAGTC | 2 cut(s) | 194, 1007 |
| MmeI | TCCRAC | 1 cut(s) | 112 |
| Mox20I | TGGCCA | 1 cut(s) | 945 |
| Mph1103I | ATGCAT | 1 cut(s) | 112 |
| MroI | TCCGGA | 1 cut(s) | 778 |
| MroXI | GAANNNNTTC | 1 cut(s) | 1018 |
| MscI | TGGCCA | 1 cut(s) | 945 |
| MseI | TTAA | 2 cut(s) | 431, 747 |
| Msp20I | TGGCCA | 1 cut(s) | 945 |
| MspA1I | CMGCKG | 1 cut(s) | 993 |
| MspI | CCGG | 2 cut(s) | 507, 779 |
| MspR9I | CCNGG | 1 cut(s) | 810 |
| MunI | CAATTG | 1 cut(s) | 702 |
| Mva1269I | GAATGC | 2 cut(s) | 441, 1182 |
| MvaI | CCWGG | 1 cut(s) | 810 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 13, 363, 507, 913, 1171 |
| NdeI | CATATG | 1 cut(s) | 12 |
| NdeII | GATC | 5 cut(s) | 127, 169, 406, 729, 1372 |
| NlaIII | CATG | 6 cut(s) | 53, 58, 195, 293, 430, 829 |
| NlaIV | GGNNCC | 5 cut(s) | 295, 582, 583, 709, 1313 |
| NsiI | ATGCAT | 1 cut(s) | 112 |
| NspI | RCATGY | 2 cut(s) | 430, 829 |
| PagI | TCATGA | 1 cut(s) | 54 |
| PceI | AGGCCT | 1 cut(s) | 819 |
| PciI | ACATGT | 1 cut(s) | 426 |
| PctI | GAATGC | 2 cut(s) | 441, 1182 |
| PdmI | GAANNNNTTC | 1 cut(s) | 1018 |
| PfeI | GAWTC | 6 cut(s) | 132, 155, 333, 370, 604, 1326 |
| PkrI | GCNGC | 2 cut(s) | 995, 1064 |
| PleI | GAGTC | 2 cut(s) | 194, 1006 |
| PpsI | GAGTC | 2 cut(s) | 194, 1006 |
| PpuMI | RGGWCCY | 1 cut(s) | 581 |
| PscI | ACATGT | 1 cut(s) | 426 |
| PsiI | TTATAA | 2 cut(s) | 482, 1043 |
| Psp1406I | AACGTT | 1 cut(s) | 1046 |
| Psp5II | RGGWCCY | 1 cut(s) | 581 |
| Psp6I | CCWGG | 1 cut(s) | 808 |
| PspFI | CCCAGC | 1 cut(s) | 880 |
| PspGI | CCWGG | 1 cut(s) | 808 |
| PspN4I | GGNNCC | 5 cut(s) | 295, 582, 583, 709, 1313 |
| PspPI | GGNCC | 3 cut(s) | 179, 581, 707 |
| PspPPI | RGGWCCY | 1 cut(s) | 581 |
| PstNI | CAGNNNCTG | 1 cut(s) | 281 |
| PsuI | RGATCY | 2 cut(s) | 406, 1372 |
| PvuII | CAGCTG | 1 cut(s) | 993 |
| RsaI | GTAC | 1 cut(s) | 848 |
| RsaNI | GTAC | 1 cut(s) | 847 |
| SaqAI | TTAA | 2 cut(s) | 431, 747 |
| SatI | GCNGC | 2 cut(s) | 994, 1063 |
| Sau3AI | GATC | 5 cut(s) | 127, 169, 406, 729, 1372 |
| Sau96I | GGNCC | 3 cut(s) | 179, 581, 707 |
| SchI | GAGTC | 2 cut(s) | 194, 1007 |
| ScrFI | CCNGG | 1 cut(s) | 810 |
| SinI | GGWCC | 2 cut(s) | 581, 707 |
| SseBI | AGGCCT | 1 cut(s) | 819 |
| SsiI | CCGC | 2 cut(s) | 374, 806 |
| SspMI | CTAG | 3 cut(s) | 416, 1010, 1370 |
| StuI | AGGCCT | 1 cut(s) | 819 |
| StyD4I | CCNGG | 1 cut(s) | 808 |
| StyI | CCWWGG | 1 cut(s) | 597 |
| TaiI | ACGT | 2 cut(s) | 768, 1049 |
| TaqI | TCGA | 2 cut(s) | 130, 345 |
| TaqII | GACCGA | 1 cut(s) | 24 |
| TfiI | GAWTC | 6 cut(s) | 132, 155, 333, 370, 604, 1326 |
| Tru1I | TTAA | 2 cut(s) | 431, 747 |
| Tru9I | TTAA | 2 cut(s) | 431, 747 |
| TscAI | CASTG | 1 cut(s) | 625 |
| TseI | GCWGC | 2 cut(s) | 993, 1062 |
| TspDTI | ATGAA | 8 cut(s) | 38, 135, 325, 600, 653, 776, 876, 1011 |
| TspGWI | ACGGA | 1 cut(s) | 193 |
| TspRI | CASTG | 1 cut(s) | 625 |
| VpaK11BI | GGWCC | 2 cut(s) | 581, 707 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 813 |
| XapI | RAATTY | 6 cut(s) | 146, 513, 863, 967, 1257, 1292 |
| XbaI | TCTAGA | 2 cut(s) | 415, 1369 |
| XceI | RCATGY | 2 cut(s) | 430, 829 |
| XmiI | GTMKAC | 1 cut(s) | 38 |
| XmnI | GAANNNNTTC | 1 cut(s) | 1018 |
| XspI | CTAG | 3 cut(s) | 416, 1010, 1370 |
| Zsp2I | ATGCAT | 1 cut(s) | 112 |