DDE superfamily endonuclease
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AcoI | YGGCCR | 1 cut(s) | 178 |
| AcsI | RAATTY | 1 cut(s) | 419 |
| AcuI | CTGAAG | 1 cut(s) | 288 |
| AdeI | CACNNNGTG | 1 cut(s) | 321 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 23 |
| AgsI | TTSAA | 5 cut(s) | 59, 68, 121, 167, 349 |
| AluBI | AGCT | 2 cut(s) | 71, 176 |
| AluI | AGCT | 2 cut(s) | 71, 176 |
| Alw21I | GWGCWC | 2 cut(s) | 109, 221 |
| AoxI | GGCC | 1 cut(s) | 178 |
| ApoI | RAATTY | 1 cut(s) | 419 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 63 |
| AsuHPI | GGTGA | 1 cut(s) | 17 |
| BalI | TGGCCA | 1 cut(s) | 180 |
| BbsI | GAAGAC | 1 cut(s) | 389 |
| Bbv12I | GWGCWC | 2 cut(s) | 109, 221 |
| BccI | CCATC | 1 cut(s) | 268 |
| BfaI | CTAG | 2 cut(s) | 173, 312 |
| BmrI | ACTGGG | 1 cut(s) | 260 |
| BmsI | GCATC | 1 cut(s) | 348 |
| BmuI | ACTGGG | 1 cut(s) | 260 |
| BpiI | GAAGAC | 1 cut(s) | 389 |
| Bpu10I | CCTNAGC | 1 cut(s) | 111 |
| BsaBI | GATNNNNATC | 1 cut(s) | 48 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 23 |
| Bse1I | ACTGG | 1 cut(s) | 255 |
| Bse8I | GATNNNNATC | 1 cut(s) | 48 |
| BseGI | GGATG | 1 cut(s) | 330 |
| BseJI | GATNNNNATC | 1 cut(s) | 48 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 23 |
| BseMII | CTCAG | 2 cut(s) | 51, 102 |
| BseNI | ACTGG | 1 cut(s) | 255 |
| BshFI | GGCC | 1 cut(s) | 180 |
| BsiHKAI | GWGCWC | 2 cut(s) | 109, 221 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 23 |
| BsmI | GAATGC | 1 cut(s) | 85 |
| BsnI | GGCC | 1 cut(s) | 180 |
| Bsp1286I | GDGCHC | 2 cut(s) | 109, 221 |
| BspANI | GGCC | 1 cut(s) | 180 |
| BspCNI | CTCAG | 2 cut(s) | 50, 103 |
| BsrI | ACTGG | 1 cut(s) | 255 |
| BstC8I | GCNNGC | 1 cut(s) | 178 |
| BstDEI | CTNAG | 2 cut(s) | 37, 111 |
| BstF5I | GGATG | 1 cut(s) | 330 |
| BstV2I | GAAGAC | 1 cut(s) | 389 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 197 |
| BsuRI | GGCC | 1 cut(s) | 180 |
| BtsCI | GGATG | 1 cut(s) | 330 |
| Cac8I | GCNNGC | 1 cut(s) | 178 |
| CviAII | CATG | 2 cut(s) | 379, 405 |
| CviJI | RGCY | 4 cut(s) | 71, 176, 180, 409 |
| CviKI_1 | RGCY | 4 cut(s) | 71, 176, 180, 409 |
| DdeI | CTNAG | 2 cut(s) | 37, 111 |
| DraIII | CACNNNGTG | 1 cut(s) | 321 |
| EaeI | YGGCCR | 1 cut(s) | 178 |
| Eco57I | CTGAAG | 1 cut(s) | 288 |
| FaeI | CATG | 2 cut(s) | 382, 408 |
| FaiI | YATR | 3 cut(s) | 380, 406, 417 |
| FatI | CATG | 2 cut(s) | 378, 404 |
| FokI | GGATG | 1 cut(s) | 337 |
| FspBI | CTAG | 2 cut(s) | 173, 312 |
| HaeIII | GGCC | 1 cut(s) | 180 |
| Hin1II | CATG | 2 cut(s) | 382, 408 |
| HinfI | GANTC | 4 cut(s) | 35, 44, 334, 385 |
| HphI | GGTGA | 1 cut(s) | 17 |
| Hpy166II | GTNNAC | 1 cut(s) | 305 |
| Hpy188I | TCNGA | 1 cut(s) | 40 |
| Hpy188III | TCNNGA | 3 cut(s) | 76, 266, 353 |
| Hpy8I | GTNNAC | 1 cut(s) | 305 |
| HpyAV | CCTTC | 1 cut(s) | 17 |
| HpyCH4V | TGCA | 2 cut(s) | 247, 404 |
| HpyF3I | CTNAG | 2 cut(s) | 37, 111 |
| Hsp92II | CATG | 2 cut(s) | 382, 408 |
| LmnI | GCTCC | 1 cut(s) | 216 |
| LpnPI | CCDG | 5 cut(s) | 123, 162, 236, 279, 309 |
| LweI | GCATC | 1 cut(s) | 348 |
| MaeI | CTAG | 2 cut(s) | 173, 312 |
| MboII | GAAGA | 2 cut(s) | 159, 394 |
| MfeI | CAATTG | 2 cut(s) | 54, 116 |
| MhlI | GDGCHC | 2 cut(s) | 109, 221 |
| MlsI | TGGCCA | 1 cut(s) | 180 |
| MluCI | AATT | 6 cut(s) | 54, 63, 116, 153, 183, 419 |
| MluNI | TGGCCA | 1 cut(s) | 180 |
| MlyI | GAGTC | 3 cut(s) | 29, 328, 379 |
| MnlI | CCTC | 1 cut(s) | 152 |
| Mox20I | TGGCCA | 1 cut(s) | 180 |
| MroXI | GAANNNNTTC | 1 cut(s) | 63 |
| MscI | TGGCCA | 1 cut(s) | 180 |
| MseI | TTAA | 1 cut(s) | 423 |
| Msp20I | TGGCCA | 1 cut(s) | 180 |
| MunI | CAATTG | 2 cut(s) | 54, 116 |
| Mva1269I | GAATGC | 1 cut(s) | 85 |
| NlaIII | CATG | 2 cut(s) | 382, 408 |
| PctI | GAATGC | 1 cut(s) | 85 |
| PdmI | GAANNNNTTC | 1 cut(s) | 63 |
| PfeI | GAWTC | 1 cut(s) | 44 |
| PleI | GAGTC | 3 cut(s) | 29, 328, 379 |
| PpsI | GAGTC | 3 cut(s) | 29, 328, 379 |
| SaqAI | TTAA | 1 cut(s) | 423 |
| SchI | GAGTC | 3 cut(s) | 29, 328, 379 |
| SduI | GDGCHC | 2 cut(s) | 109, 221 |
| SetI | ASST | 6 cut(s) | 73, 112, 163, 178, 298, 310 |
| SfaNI | GCATC | 1 cut(s) | 348 |
| Sse9I | AATT | 6 cut(s) | 54, 63, 116, 153, 183, 419 |
| SspMI | CTAG | 2 cut(s) | 173, 312 |
| TaqI | TCGA | 2 cut(s) | 21, 337 |
| TasI | AATT | 6 cut(s) | 54, 63, 116, 153, 183, 419 |
| TfiI | GAWTC | 1 cut(s) | 44 |
| Tru1I | TTAA | 1 cut(s) | 423 |
| Tru9I | TTAA | 1 cut(s) | 423 |
| TspDTI | ATGAA | 4 cut(s) | 138, 254, 395, 432 |
| XapI | RAATTY | 1 cut(s) | 419 |
| XmnI | GAANNNNTTC | 1 cut(s) | 63 |
| XspI | CTAG | 2 cut(s) | 173, 312 |