RchiOBHm_Chr7g0199281
MYB Family

Myb/SANT-like DNA-binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0000240
Physical Location & Seq
Forward (+)
17327874 .. 17334623
6750 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ17832

Sequence Viewer

Length: 1317 bp
ATGAATTTTGACTTCATGCCAATTATGTCCTCCTCCTTTTCTGATAATTACATCGAAACAACAGAACCCTATCTTTTCTCCATCTCTTTGCCGTCATTTTCATTATCACTCCACATCTCTTCATCTCCTCCTTCCTCTTCTTCAATTCGTGGATTCTCGCCGGCCTCGCCTCCTTCAATTCGTGGGTTCCATCTCTTTGCCGTCATTATCGTTATTATTCCACATCTCCTCCTTCCTCTTCTTCAATTCGTGGATTCTCTCTACAATTTGGGTATTCTGGGTTATGTTTCTTCTCTGAAGATAATCTATCAAACAGAACCCTATTCCTCTGATTCCTCCATAGCTGCACAAAAAAGAGTAAGGTATATGGGAAAGAAGAAATCCACCAATACCGAATATGAGGGATCTGGAAAACAAAAGGCCACATGGCCTGATGAGGTTGTAGCTATATTTTGTGATATAGATGTTAAGGAAGTGGCCAAAGGAAATAGGCCCAGTACGCATTTTGATAAAAAAGGATGGACAAATGTTGTGTTAGCCTTTAAGGAGTTAACCGGAAGGGATTATGATAAAAAGCAATTGAAAAATAAATGGGATTCGCTTAAAAATGATTGGAAATTGTGGAGTTCGCTGTTGCATAAGGAAACTGGTATTGGATGGGATCCGGCTAGGAAGACTGTCGATGCACCGGCTGAATGGTGGGAAACCAAAATTCTGATCAATCCAGAGTATCGCAAATTTCGCGAAGTGGGAGTTAGTCCTGACATGATGGCTGTTTATGATAACATGTTCAAGGGTAGCACAGCCCTAGGTAACAATGTCATGATTCCCTCAGAAACTATAGATATTGAAGAGGTTGTGGAGGATTCTGAGCATAATATTATTTCTGGAGATGATGAAGAGGATTATGAGCAAGGTAATGAATGTAGAGGGAAAAAGAGAACAAGTGTGGAGCGCCAAACAGAGGCTGATAAAGATAAAAAAGGAAAAGGAGTTATGGGAGGGCCAAAGGGGAAGAAAGAAAAGGTGGGAGGTGCGGCCAAATTGTCTAAACAAATAGATCGGCTTGTTGATGTAGTCGAGAGTAGGAGTACAGCCACCTCTGTTCGTGATAATGGTACCTCACATGGAACTAGTATTCAGGAGGTGATGAAAGTTGTAGCAACATTACCAGGAGCGGAAACTGGTACCAAGTTATGGTGGTTTGCAACGGAGCTGTTCTGCTCTCAAGAGAAGCGAGAGATGTTTTCTATTATGACAGATGTTGATCTCAAGCTCCAGTTTCTGATTCTTAATCAGAAAAAAGATGAAAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Myb_DNA-bind_3

Protein Analysis

438

Amino Acids

49.25

Weight (kDa)

6.08

Isoelectric Point (pI)

41.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Myb_DNA-bind_3 PF12776 143 - 235 2.1e-21 Myb/SANT-like DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000240)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G11300
fragaria_vesca FvH4_1g19130 FvH4_1g28413 FvH4_2g11122 FvH4_3g19652 FvH4_3g29921 FvH4_4g07761 FvH4_4g20923 FvH4_5g23913 FvH4_5g23913 FvH4_6g38850 FvH4_6g39920 FvH4_6g53022 FvH4_7g00070 FvH4_7g08972 FvH4_7g12221
malus_domestica MD06G1090800.v1.1 MD15G1314200.v1.1 MD16G1231800.v1.1
prunus_persica Prupe.1G173000_v2.0.a1 Prupe.4G113600_v2.0.a1 Prupe.5G020800_v2.0.a1 Prupe.5G020800_v2.0.a1 Prupe.5G020800_v2.0.a1 Prupe.5G207000_v2.0.a1 Prupe.6G155900_v2.0.a1 Prupe.8G016500_v2.0.a1
pyrus_communis pycom03g16230 pycom04g16130 pycom05g03970 pycom05g10500 pycom08g06480 pycom08g11770 pycom12g11350 pycom12g18510 pycom13g02550 pycom13g13780 pycom13g27450 pycom13g27460 pycom15g26130 pycom15g27470
rosa_chinensis RchiOBHm_Chr2g0103961 RchiOBHm_Chr2g0128681 RchiOBHm_Chr2g0153151 RchiOBHm_Chr3g0459461 RchiOBHm_Chr4g0406011 RchiOBHm_Chr4g0428601 RchiOBHm_Chr5g0029731 RchiOBHm_Chr5g0069971 RchiOBHm_Chr6g0253171 RchiOBHm_Chr6g0254701 RchiOBHm_Chr7g0199281
rosa_laevigata RLG00000002281 RLG00000007586 RLG00000008509 RLG00000008888 RLG00000009501 RLG00000012763
rosa_multiflora Rmu_sc0000008.1_g000009 Rmu_sc0000079.1_g000058 Rmu_sc0000117.1_g000008 Rmu_sc0000151.1_g000009 Rmu_sc0000166.1_g000053 Rmu_sc0000240.1_g000064 Rmu_sc0000435.1_g000013 Rmu_sc0000552.1_g000019 Rmu_sc0000776.1_g000078 Rmu_sc0001597.1_g000012 Rmu_sc0001759.1_g000017 Rmu_sc0002137.1_g000011 Rmu_sc0002933.1_g000006 Rmu_sc0002938.1_g000049 Rmu_sc0003257.1_g000014 Rmu_sc0003465.1_g000054 Rmu_sc0003505.1_g000025 Rmu_sc0003526.1_g000016 Rmu_sc0003829.1_g000021 Rmu_sc0003855.1_g000002 Rmu_sc0005514.1_g000003 Rmu_sc0005514.1_g000004 Rmu_sc0007820.1_g000001 Rmu_sc0008322.1_g000016 Rmu_sc0008636.1_g000003 Rmu_sc0008812.1_g000009 Rmu_sc0010960.1_g000003 Rmu_sc0011753.1_g000001 Rmu_sc0013017.1_g000001 Rmu_sc0013176.1_g000005 Rmu_sc0015928.1_g000001 Rmu_sc0020751.1_g000003 Rmu_sc0038865.1_g000001 Rmu_ssc0000089.1_g000008 Rmu_ssc0000164.1_g000007 Rmu_ssc0000388.1_g000013 Rmu_ssc0000400.1_g000076 Rmu_ssc0000486.1_g000012
rosa_roxburghii Rroxscaffold_1G00005880 Rroxscaffold_1G00051030 Rroxscaffold_2G00107440 Rroxscaffold_2G00115370 Rroxscaffold_2G00115870 Rroxscaffold_2G00146360 Rroxscaffold_3G00228870 Rroxscaffold_3G00230350 Rroxscaffold_3G00246190 Rroxscaffold_6G00423350 Rroxscaffold_7G00157820 Rroxscaffold_7G00184840 Rroxscaffold_7G00186490
rosa_rugosa Rorug02G0248700 Rorug02G0279900 Rorug02G0285600 Rorug02G0285700 Rorug03G0068700 Rorug03G0077100 Rorug03G0247700 Rorug03G0304800 Rorug04G0059600 Rorug05G0104000 Rorug05G0104000 Rorug05G0192000 Rorug05G0247800 Rorug05G0248000 Rorug06G0064100 Rorug06G0244800
rosa_samantha Rh4BG200400 Rh4CG094600 Rh4CG114400 Rh5BG465300 Rh6AG057500
rosa_wichuraiana Rw1G014470 Rw2G026930 Rw5G042730 Rw6G021090 Rw6G022960 Rw6G025470 Rw6G034450 Rw7G034300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 1118, 1187
AccB1I GGYRCC 2 cut(s) 1118, 1187
AccB7I CCANNNNNTGG 1 cut(s) 1197
AccBSI CCGCTC 1 cut(s) 1178
AccII CGCG 1 cut(s) 744
AciI CCGC 2 cut(s) 1037, 1178
AclWI GGATC 3 cut(s) 412, 656, 669
AcoI YGGCCR 2 cut(s) 477, 1038
AcsI RAATTY 3 cut(s) 4, 711, 737
AcuI CTGAAG 1 cut(s) 317
AfaI GTAC 4 cut(s) 499, 1093, 1120, 1189
AfiI CCNNNNNNNGG 2 cut(s) 964, 1197
AflIII ACRYGT 1 cut(s) 786
AgsI TTSAA 6 cut(s) 144, 177, 245, 583, 793, 851
AhlI ACTAGT 1 cut(s) 1133
AjnI CCWGG 1 cut(s) 1171
AjuI GAANNNNNNNTTGG 2 cut(s) 636, 668
AluBI AGCT 4 cut(s) 344, 446, 1216, 1276
AluI AGCT 4 cut(s) 344, 446, 1216, 1276
AlwI GGATC 3 cut(s) 412, 656, 669
AlwNI CAGNNNCTG 2 cut(s) 968, 1285
AoxI GGCC 7 cut(s) 162, 420, 428, 477, 491, 1004, 1038
ApeKI GCWGC 1 cut(s) 344
ApoI RAATTY 3 cut(s) 4, 711, 737
Asp718I GGTACC 2 cut(s) 1118, 1187
AspA2I CCTAGG 1 cut(s) 808
AspLEI GCGC 1 cut(s) 957
AspS9I GGNCC 2 cut(s) 492, 1004
AsuHPI GGTGA 1 cut(s) 1159
AvrII CCTAGG 1 cut(s) 808
BalI TGGCCA 1 cut(s) 479
BamHI GGATCC 1 cut(s) 661
BanI GGYRCC 2 cut(s) 1118, 1187
BbsI GAAGAC 1 cut(s) 680
BbvI GCAGC 1 cut(s) 331
BccI CCATC 5 cut(s) 89, 198, 513, 651, 763
BceAI ACGGC 2 cut(s) 76, 185
BciT130I CCWGG 1 cut(s) 1173
BclI TGATCA 1 cut(s) 717
BcuI ACTAGT 1 cut(s) 1133
BfaI CTAG 3 cut(s) 669, 809, 1134
BfmI CTRYAG 1 cut(s) 840
BfoI RGCGCY 1 cut(s) 958
BisI GCNGC 2 cut(s) 345, 1038
BlnI CCTAGG 1 cut(s) 808
BlsI GCNGC 2 cut(s) 346, 1039
Bme1390I CCNGG 1 cut(s) 1173
BmgT120I GGNCC 2 cut(s) 492, 1004
BmiI GGNNCC 4 cut(s) 188, 663, 1120, 1189
BmrFI CCNGG 1 cut(s) 1173
BmrI ACTGGG 1 cut(s) 489
BmsI GCATC 1 cut(s) 673
BmuI ACTGGG 1 cut(s) 489
BpiI GAAGAC 1 cut(s) 680
BpmI CTGGAG 2 cut(s) 909, 1262
BpuEI CTTGAG 2 cut(s) 1212, 1256
BsaBI GATNNNNATC 1 cut(s) 1266
BsaJI CCNNGG 1 cut(s) 808
BsaWI WCCGGW 1 cut(s) 554
BsaXI ACNNNNNCTCC 2 cut(s) 213, 243
Bsc4I CCNNNNNNNGG 2 cut(s) 964, 1197
Bse118I RCCGGY 2 cut(s) 160, 688
Bse1I ACTGG 4 cut(s) 495, 652, 1189, 1279
Bse8I GATNNNNATC 1 cut(s) 1266
BseBI CCWGG 1 cut(s) 1173
BseDI CCNNGG 1 cut(s) 808
BseGI GGATG 2 cut(s) 524, 662
BseJI GATNNNNATC 1 cut(s) 1266
BseLI CCNNNNNNNGG 2 cut(s) 964, 1197
BseMII CTCAG 2 cut(s) 846, 861
BseNI ACTGG 4 cut(s) 495, 652, 1189, 1279
BseRI GAGGAG 3 cut(s) 22, 117, 218
BseXI GCAGC 1 cut(s) 331
BsgI GTGCAG 1 cut(s) 330
Bsh1236I CGCG 1 cut(s) 744
BshFI GGCC 7 cut(s) 164, 422, 430, 479, 493, 1006, 1040
BshNI GGYRCC 2 cut(s) 1118, 1187
BsiSI CCGG 4 cut(s) 161, 555, 665, 689
BslI CCNNNNNNNGG 2 cut(s) 964, 1197
BsnI GGCC 7 cut(s) 164, 422, 430, 479, 493, 1006, 1040
Bsp143I GATC 5 cut(s) 404, 661, 717, 1060, 1267
Bsp68I TCGCGA 1 cut(s) 744
BspACI CCGC 2 cut(s) 1037, 1178
BspANI GGCC 7 cut(s) 164, 422, 430, 479, 493, 1006, 1040
BspCNI CTCAG 2 cut(s) 845, 862
BspFNI CGCG 1 cut(s) 744
BspHI TCATGA 1 cut(s) 822
BspLI GGNNCC 4 cut(s) 188, 663, 1120, 1189
BspPI GGATC 3 cut(s) 412, 656, 669
BspT107I GGYRCC 2 cut(s) 1118, 1187
BsrBI CCGCTC 1 cut(s) 1178
BsrFI RCCGGY 2 cut(s) 160, 688
BsrI ACTGG 4 cut(s) 495, 652, 1189, 1279
BssAI RCCGGY 2 cut(s) 160, 688
BssECI CCNNGG 1 cut(s) 808
BssMI GATC 5 cut(s) 404, 661, 717, 1060, 1267
BssT1I CCWWGG 1 cut(s) 808
Bst2UI CCWGG 1 cut(s) 1173
Bst4CI ACNGT 1 cut(s) 679
Bst6I CTCTTC 5 cut(s) 124, 142, 243, 846, 894
BstC8I GCNNGC 1 cut(s) 162
BstDEI CTNAG 2 cut(s) 832, 870
BstF5I GGATG 2 cut(s) 524, 662
BstFNI CGCG 1 cut(s) 744
BstH2I RGCGCY 1 cut(s) 958
BstHHI GCGC 1 cut(s) 957
BstKTI GATC 5 cut(s) 407, 664, 720, 1063, 1270
BstMBI GATC 5 cut(s) 404, 661, 717, 1060, 1267
BstMWI GCNNNNNNNGC 3 cut(s) 166, 499, 741
BstNI CCWGG 1 cut(s) 1173
BstNSI RCATGY 1 cut(s) 790
BstSCI CCNGG 1 cut(s) 1171
BstSFI CTRYAG 1 cut(s) 840
BstUI CGCG 1 cut(s) 744
BstV1I GCAGC 1 cut(s) 331
BstV2I GAAGAC 1 cut(s) 680
BstX2I RGATCY 2 cut(s) 404, 661
BstYI RGATCY 2 cut(s) 404, 661
BsuRI GGCC 7 cut(s) 164, 422, 430, 479, 493, 1006, 1040
BtsCI GGATG 2 cut(s) 524, 662
BtuMI TCGCGA 1 cut(s) 744
Cac8I GCNNGC 1 cut(s) 162
CaiI CAGNNNCTG 2 cut(s) 968, 1285
CciI TCATGA 1 cut(s) 822
CfoI GCGC 1 cut(s) 957
Cfr10I RCCGGY 2 cut(s) 160, 688
Cfr13I GGNCC 2 cut(s) 492, 1004
Csp6I GTAC 4 cut(s) 498, 1092, 1119, 1188
CviAII CATG 6 cut(s) 16, 426, 766, 787, 823, 1127
CviQI GTAC 4 cut(s) 498, 1092, 1119, 1188
DdeI CTNAG 2 cut(s) 832, 870
DpnI GATC 5 cut(s) 406, 663, 719, 1062, 1269
DpnII GATC 5 cut(s) 404, 661, 717, 1060, 1267
EaeI YGGCCR 2 cut(s) 477, 1038
Eam1104I CTCTTC 5 cut(s) 124, 142, 243, 846, 894
EarI CTCTTC 5 cut(s) 124, 142, 243, 846, 894
Eco130I CCWWGG 1 cut(s) 808
Eco57I CTGAAG 1 cut(s) 317
EcoRII CCWGG 1 cut(s) 1171
EcoT14I CCWWGG 1 cut(s) 808
ErhI CCWWGG 1 cut(s) 808
FaeI CATG 6 cut(s) 19, 429, 769, 790, 826, 1130
FatI CATG 6 cut(s) 15, 425, 765, 786, 822, 1126
FbaI TGATCA 1 cut(s) 717
Fnu4HI GCNGC 2 cut(s) 345, 1038
FokI GGATG 2 cut(s) 531, 669
Fsp4HI GCNGC 2 cut(s) 345, 1038
FspBI CTAG 3 cut(s) 669, 809, 1134
GlaI GCGC 1 cut(s) 956
GluI GCNGC 2 cut(s) 345, 1038
GsuI CTGGAG 2 cut(s) 909, 1262
HaeII RGCGCY 1 cut(s) 958
HaeIII GGCC 7 cut(s) 164, 422, 430, 479, 493, 1006, 1040
HapII CCGG 4 cut(s) 161, 555, 665, 689
HhaI GCGC 1 cut(s) 957
Hin1II CATG 6 cut(s) 19, 429, 769, 790, 826, 1130
Hin6I GCGC 1 cut(s) 955
HinP1I GCGC 1 cut(s) 955
HincII GTYRAC 1 cut(s) 552
HindII GTYRAC 1 cut(s) 552
HinfI GANTC 7 cut(s) 153, 254, 332, 596, 826, 866, 1288
HpaI GTTAAC 1 cut(s) 552
HpaII CCGG 4 cut(s) 161, 555, 665, 689
HphI GGTGA 1 cut(s) 1159
Hpy166II GTNNAC 1 cut(s) 552
Hpy188I TCNGA 8 cut(s) 43, 297, 331, 717, 835, 871, 1287, 1299
Hpy8I GTNNAC 1 cut(s) 552
HpyAV CCTTC 4 cut(s) 141, 183, 242, 552
HpyCH4III ACNGT 1 cut(s) 679
HpyCH4V TGCA 4 cut(s) 347, 637, 686, 1208
HpyF10VI GCNNNNNNNGC 3 cut(s) 166, 499, 741
HpyF3I CTNAG 2 cut(s) 832, 870
Hsp92II CATG 6 cut(s) 19, 429, 769, 790, 826, 1130
HspAI GCGC 1 cut(s) 955
KpnI GGTACC 2 cut(s) 1122, 1191
KroI GCCGGC 1 cut(s) 160
KroNI GCCGGC 1 cut(s) 162
Ksp22I TGATCA 1 cut(s) 717
KspAI GTTAAC 1 cut(s) 552
Kzo9I GATC 5 cut(s) 404, 661, 717, 1060, 1267
LmnI GCTCC 4 cut(s) 952, 1175, 1213, 1281
Lsp1109I GCAGC 1 cut(s) 331
LweI GCATC 1 cut(s) 673
MaeI CTAG 3 cut(s) 669, 809, 1134
MaeIII GTNAC 1 cut(s) 812
MalI GATC 5 cut(s) 406, 663, 719, 1062, 1269
MbiI CCGCTC 1 cut(s) 1178
MboI GATC 5 cut(s) 404, 661, 717, 1060, 1267
MfeI CAATTG 1 cut(s) 578
MflI RGATCY 2 cut(s) 404, 661
MlsI TGGCCA 1 cut(s) 479
MluNI TGGCCA 1 cut(s) 479
Mox20I TGGCCA 1 cut(s) 479
MroNI GCCGGC 1 cut(s) 160
MscI TGGCCA 1 cut(s) 479
MseI TTAA 6 cut(s) 468, 543, 551, 603, 1293, 1315
Msp20I TGGCCA 1 cut(s) 479
MspI CCGG 4 cut(s) 161, 555, 665, 689
MspR9I CCNGG 1 cut(s) 1173
MunI CAATTG 1 cut(s) 578
MvaI CCWGG 1 cut(s) 1173
MvnI CGCG 1 cut(s) 744
MwoI GCNNNNNNNGC 3 cut(s) 166, 499, 741
NaeI GCCGGC 1 cut(s) 162
NdeII GATC 5 cut(s) 404, 661, 717, 1060, 1267
NgoMIV GCCGGC 1 cut(s) 160
NlaIII CATG 6 cut(s) 19, 429, 769, 790, 826, 1130
NlaIV GGNNCC 4 cut(s) 188, 663, 1120, 1189
NruI TCGCGA 1 cut(s) 744
NspI RCATGY 1 cut(s) 790
PagI TCATGA 1 cut(s) 822
PciI ACATGT 1 cut(s) 786
PdiI GCCGGC 1 cut(s) 162
PfeI GAWTC 7 cut(s) 153, 254, 332, 596, 826, 866, 1288
PflMI CCANNNNNTGG 1 cut(s) 1197
PkrI GCNGC 2 cut(s) 346, 1039
PscI ACATGT 1 cut(s) 786
Psp6I CCWGG 1 cut(s) 1171
PspGI CCWGG 1 cut(s) 1171
PspN4I GGNNCC 4 cut(s) 188, 663, 1120, 1189
PspPI GGNCC 2 cut(s) 492, 1004
PstNI CAGNNNCTG 2 cut(s) 968, 1285
PsuI RGATCY 2 cut(s) 404, 661
RruI TCGCGA 1 cut(s) 744
RsaI GTAC 4 cut(s) 499, 1093, 1120, 1189
RsaNI GTAC 4 cut(s) 498, 1092, 1119, 1188
SaqAI TTAA 6 cut(s) 468, 543, 551, 603, 1293, 1315
SatI GCNGC 2 cut(s) 345, 1038
Sau3AI GATC 5 cut(s) 404, 661, 717, 1060, 1267
Sau96I GGNCC 2 cut(s) 492, 1004
ScrFI CCNGG 1 cut(s) 1173
SfaNI GCATC 1 cut(s) 673
SfcI CTRYAG 1 cut(s) 840
SmlI CTYRAG 2 cut(s) 1227, 1271
SmoI CTYRAG 2 cut(s) 1227, 1271
SpeI ACTAGT 1 cut(s) 1133
SsiI CCGC 2 cut(s) 1037, 1178
SspI AATATT 1 cut(s) 880
SspMI CTAG 3 cut(s) 669, 809, 1134
StyD4I CCNGG 1 cut(s) 1171
StyI CCWWGG 1 cut(s) 808
TaaI ACNGT 1 cut(s) 679
TaqI TCGA 3 cut(s) 54, 681, 1080
TatI WGTACW 1 cut(s) 1091
TauI GCSGC 1 cut(s) 1040
TfiI GAWTC 7 cut(s) 153, 254, 332, 596, 826, 866, 1288
Tru1I TTAA 6 cut(s) 468, 543, 551, 603, 1293, 1315
Tru9I TTAA 6 cut(s) 468, 543, 551, 603, 1293, 1315
TseI GCWGC 1 cut(s) 344
TspDTI ATGAA 7 cut(s) 4, 17, 90, 111, 912, 936, 1166
TspGWI ACGGA 1 cut(s) 1226
Van91I CCANNNNNTGG 1 cut(s) 1197
XapI RAATTY 3 cut(s) 4, 711, 737
XceI RCATGY 1 cut(s) 790
XmaJI CCTAGG 1 cut(s) 808
XspI CTAG 3 cut(s) 669, 809, 1134
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.