FvH4_6g45770

Agglutinin domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0000217
Physical Location & Seq
Reverse (-)
35050562 .. 35053316
2755 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g45770.t1

Sequence Viewer

Length: 1617 bp
ATGTCGATCCTGTTACCACCCACAATTGCTTTGAGATCTCACGCGTTCGACACTTACTTGAGCTACAGCAATGATTCTGATTCGGAGACGCATGGCTTTCTCAAAGTGAACGGAGAAATTGTCATCAGCAGGTTGGCAAAGTTCCAAGTAGTATCAGCCACTATTGGGACGGGTCTAGTACATATAAGAAGCAGTCACAACAGAAAGTTCCTGCGAAGGAGAATTGAGGGTACTTTATGGAGCGGTGATATATACCTTTCTCCTGACGCTGATGAACCAGAGGAGGATCAGTCCAAATGGTCGTGTACACTGTTTGAGCCTAGACTATATGCACCTGGCAACCCCGTTCAGGTCCGGTTTATCCATGTCCAAACCAAACGGTATGCAACGGCCATGGAAGAGGTACGAGGTGGAGAACGGTTCTTTGGATTAACTGAAGTTCATTCTTCCGCGAGTTGGATCTTTGATGTCATTGACTTGGAGTCAATGGTGATATTGCCAAAGCATGTGGCCTTCAAAGGCCACACCGGCAATTATCTTAAGCTTGTGGAGGATGCAAAGCACAGGTTCATTTCCACCGATGAGGGGCTTCCCGAGTCCTGGTACGAGGTGGACACTGATCGGCACGGAAGGGTGGGGATCATGTCTCGCCAAGACAAGAAGCATTGGTATGACATGAATGGCTTCATTTCCTCCCGCACAAGGGATTTGAGCACACAACCCTTCACGTTGTTTTCGGCGGTGAAGGTGAACAGTAATACGGTAGCTCTCAAAAGCATGAGCACGGACCAGTTTTGCGCTCAAACGAACCATGCATTGGGAACCGACCTCCTTACTGCACCATTCACCAGCATCGCAGCATCCACACACCTTAGGCTGGAAGAGCTTGTTCTCTCTAGGAGAATCTACGAAACGAGCTTCGACCTCGCCAATGCCGTAATCTACGGCGAGACTCCGATCATCATGGCCAGCGCAAGCGCCAGCAACAATACTTCAGCAGATAACACGGTGGAATTGAAGTTCGAGTACAAGGAGAGTCAGAGCAGCACGTGGAGTTCGAGCCACTCGTGGATGGTGGGGGTGAGTCTCACCGCCGAATTCAAAGTTCCGTTCATCGGCGGTACCGAGGTCACCGCTGAAACACAGTTTTCCGGGTCCTATGATTGGGGAACAACTATTACTTCTGAAACCACATTGGGGACTACTTACACGGCTACTGTACCGGCATACTCTTCTATCATTGTAAGCCTGTTGGCCACTAAGGGAACTTGCGACGTAAGTTTCTCCTATTATCAGCGGGACGTGCTTTATGACGGTTCAACCGAAATCTTCAGGAAAGATGATGGGTTGTTCACGGGCATCAACACATACAAGTTCCACTACGTGGTTACAACAACGAAACAGTCGTCGGAATCTACAACCAGAGTTGAGAGGTTTCCTGACCCATTGATCGACCCCACCGTAGAGGAAAACCTACCTCTACCCGAGCTGTGTGGATCAACCACCACCCCGGAACTAGAGTTATCATTGCTGCATGTAGAAGAGAAGACTATCCAAGATGCAGCAACTCTGCCATTGGAGACCAACCAAGAAGTTGGAGAATCCTTGGCTTGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aerolysin Agglutinin ETX_MTX2 Fascin

Protein Analysis

539

Amino Acids

60.08

Weight (kDa)

5.14

Isoelectric Point (pI)

44.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Agglutinin PF07468 5 - 132 8.8e-13 Agglutinin domain
Aerolysin PF01117 306 - 437 4.5e-08 Aerolysin toxin
ETX_MTX2 PF03318 317 - 424 9e-07 Clostridium epsilon toxin ETX/Bacillus mosquitocidal toxin MTX2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000217)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g17710 FvH4_6g45590 FvH4_6g45591 FvH4_6g45770 FvH4_6g45770 FvH4_6g46030 FvH4_6g46040 FvH4_7g07020 FvH4_7g07071 FvH4_7g07080
malus_domestica MD01G1104300.v1.1 MD02G1240000.v1.1 MD02G1240200.v1.1 MD03G1201400.v1.1 MD03G1201500.v1.1 MD07G1171100.v1.1
prunus_persica Prupe.2G087400_v2.0.a1 Prupe.2G097500_v2.0.a1 Prupe.2G097700_v2.0.a1 Prupe.2G097800_v2.0.a1 Prupe.3G153100_v2.0.a1 Prupe.3G153300_v2.0.a1 Prupe.3G153600_v2.0.a1 Prupe.5G237200_v2.0.a1
pyrus_communis pycom03g15270 pycom06g20700 pycom07g06030 pycom10g01810 pycom10g01820 pycom10g12090 pycom14g20090 pycom14g20100 pycom14g20110 pycom14g20120
rosa_chinensis RchiOBHm_Chr1g0336971 RchiOBHm_Chr1g0337031 RchiOBHm_Chr2g0163571 RchiOBHm_Chr2g0163591 RchiOBHm_Chr2g0163631 RchiOBHm_Chr2g0163661 RchiOBHm_Chr2g0163671 RchiOBHm_Chr2g0163691 RchiOBHm_Chr2g0163741 RchiOBHm_Chr5g0011821 RchiOBHm_Chr5g0011831 RchiOBHm_Chr5g0011841 RchiOBHm_Chr5g0053101 RchiOBHm_Chr5g0072501
rosa_laevigata RLG00000010009 RLG00000021413 RLG00000029310 RLG00000029318 RLG00000036313
rosa_multiflora Rmu_co7975848.1_g000001 Rmu_co8020886.1_g000001 Rmu_co8136580.1_g000001 Rmu_co8177372.1_g000001 Rmu_co8207014.1_g000001 Rmu_co8232187.1_g000001 Rmu_co8277595.1_g000001 Rmu_co8389219.1_g000001 Rmu_co8427877.1_g000001 Rmu_co8467587.1_g000001 Rmu_sc0001475.1_g000015 Rmu_sc0001475.1_g000016 Rmu_sc0001475.1_g000017 Rmu_sc0003462.1_g000002 Rmu_sc0003462.1_g000003 Rmu_sc0003484.1_g000002 Rmu_sc0004918.1_g000004 Rmu_sc0004918.1_g000006 Rmu_sc0004918.1_g000007 Rmu_sc0004918.1_g000009 Rmu_sc0006596.1_g000003 Rmu_sc0006596.1_g000005 Rmu_sc0006596.1_g000010 Rmu_sc0008676.1_g000021 Rmu_sc0014330.1_g000002 Rmu_sc0016133.1_g000002 Rmu_sc0016133.1_g000003 Rmu_sc0016133.1_g000004 Rmu_sc0026453.1_g000003 Rmu_sc0026453.1_g000004 Rmu_sc0030660.1_g000001
rosa_roxburghii Rroxscaffold_1G00008700 Rroxscaffold_1G00064740 Rroxscaffold_2G00086950 Rroxscaffold_2G00087100 Rroxscaffold_2G00087140 Rroxscaffold_4G00302080 Rroxscaffold_4G00315020 Rroxscaffold_4G00315170 Rroxscaffold_5G00358800
rosa_rugosa Rorug01G0129200 Rorug01G0130600 Rorug01G0130700 Rorug02G0503700 Rorug02G0503800 Rorug02G0503900 Rorug02G0504000 Rorug02G0504300 Rorug05G0268200 Rorug05G0418700 Rorug05G0418800
rosa_samantha Rh1AG151100 Rh1AG151300 Rh1BG118400 Rh1BG120600 Rh1CG141500 Rh1CG142500 Rh1DG157300 Rh1DG158500 Rh2AG570600 Rh2AG570800 Rh2AG571200 Rh2AG571300 Rh2AG571500 Rh2AG571900 Rh2AG572000 Rh2AG572200 Rh2CG552600 Rh2CG552800 Rh2CG553000 Rh2CG553300 Rh2CG553400 Rh2CG553700 Rh5CG100400 Rh5CG100500 Rh5CG100600 Rh5CG100700 Rh5CG519100 Rh5DG086600 Rh5DG086700 Rh5DG086800 Rh5DG086900 Rh5DG087000 Rh5DG087100 Rh5DG367600 Rh5DG507300
rosa_wichuraiana Rw1G012130 Rw1G012240 Rw1G012380 Rw2G047240 Rw2G047260 Rw2G047270 Rw2G047290 Rw5G007940 Rw5G044120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 120
Acc65I GGTACC 1 cut(s) 1121
AccB1I GGYRCC 1 cut(s) 1121
AccB7I CCANNNNNTGG 2 cut(s) 817, 1384
AccBSI CCGCTC 1 cut(s) 243
AccII CGCG 2 cut(s) 44, 452
AciI CCGC 8 cut(s) 243, 450, 697, 740, 1092, 1119, 1134, 1297
AclWI GGATC 4 cut(s) 294, 467, 647, 1504
AcoI YGGCCR 3 cut(s) 390, 966, 1254
AcsI RAATTY 1 cut(s) 1097
AcuI CTGAAG 3 cut(s) 456, 978, 1315
AcvI CACGTG 1 cut(s) 1050
AdeI CACNNNGTG 1 cut(s) 1384
AfaI GTAC 8 cut(s) 180, 232, 307, 405, 605, 1028, 1123, 1221
AflII CTTAAG 1 cut(s) 539
AflIII ACRYGT 1 cut(s) 42
AgsI TTSAA 4 cut(s) 517, 1018, 1102, 1320
AhdI GACNNNNNGTC 1 cut(s) 481
AjiI CACGTC 1 cut(s) 1303
AjnI CCWGG 2 cut(s) 334, 599
AjuI GAANNNNNNNTTGG 4 cut(s) 408, 440, 800, 832
AloI GAACNNNNNNTCC 2 cut(s) 1004, 1036
AluBI AGCT 6 cut(s) 63, 544, 767, 886, 918, 1489
AluI AGCT 6 cut(s) 63, 544, 767, 886, 918, 1489
Alw21I GWGCWC 2 cut(s) 716, 785
Alw26I GTCTC 5 cut(s) 80, 651, 944, 1091, 1574
AlwI GGATC 4 cut(s) 294, 467, 647, 1504
Ama87I CYCGRG 2 cut(s) 593, 1484
AoxI GGCC 5 cut(s) 390, 510, 520, 966, 1254
ApeKI GCWGC 4 cut(s) 857, 1044, 1531, 1562
ApoI RAATTY 1 cut(s) 1097
ArsI GACNNNNNNTTYG 2 cut(s) 1391, 1423
Asp700I GAANNNNTTC 1 cut(s) 683
Asp718I GGTACC 1 cut(s) 1121
AspLEI GCGC 3 cut(s) 800, 974, 980
AspS9I GGNCC 3 cut(s) 352, 787, 1155
AsuC2I CCSGG 2 cut(s) 1153, 1511
AsuHPI GGTGA 8 cut(s) 257, 502, 754, 760, 838, 1081, 1093, 1123
AvaI CYCGRG 2 cut(s) 593, 1484
AvaII GGWCC 3 cut(s) 352, 787, 1155
AxyI CCTNAGG 1 cut(s) 872
BaeI ACNNNNGTAYC 2 cut(s) 1113, 1146
BalI TGGCCA 2 cut(s) 968, 1256
BanI GGYRCC 1 cut(s) 1121
BarI GAAGNNNNNNTAC 2 cut(s) 1010, 1042
BauI CACGAG 1 cut(s) 1066
BbrPI CACGTG 1 cut(s) 1050
BbsI GAAGAC 1 cut(s) 1553
Bbv12I GWGCWC 2 cut(s) 716, 785
BbvI GCAGC 4 cut(s) 869, 1056, 1518, 1574
BccI CCATC 2 cut(s) 1066, 1337
BceAI ACGGC 4 cut(s) 405, 920, 961, 1227
BciT130I CCWGG 2 cut(s) 336, 601
BcnI CCSGG 2 cut(s) 1153, 1511
BcoDI GTCTC 5 cut(s) 80, 651, 944, 1091, 1574
BfaI CTAG 4 cut(s) 176, 321, 897, 1517
BfmI CTRYAG 1 cut(s) 64
BfoI RGCGCY 1 cut(s) 981
BfrI CTTAAG 1 cut(s) 539
BfuAI ACCTGC 1 cut(s) 120
BglI GCCNNNNNGGC 1 cut(s) 528
BglII AGATCT 1 cut(s) 35
BisI GCNGC 4 cut(s) 858, 1045, 1532, 1563
BlsI GCNGC 4 cut(s) 859, 1046, 1533, 1564
Bme1390I CCNGG 4 cut(s) 336, 601, 1153, 1511
Bme18I GGWCC 3 cut(s) 352, 787, 1155
BmeRI GACNNNNNGTC 1 cut(s) 481
BmeT110I CYCGRG 2 cut(s) 593, 1484
BmgBI CACGTC 1 cut(s) 1303
BmgT120I GGNCC 3 cut(s) 352, 787, 1155
BmiI GGNNCC 3 cut(s) 823, 1123, 1156
BmrFI CCNGG 4 cut(s) 336, 601, 1153, 1511
BmsI GCATC 5 cut(s) 544, 861, 869, 1368, 1549
BpiI GAAGAC 1 cut(s) 1553
BpuEI CTTGAG 1 cut(s) 79
BpuMI CCSGG 2 cut(s) 1153, 1511
BsaAI YACGTR 2 cut(s) 1050, 1384
BsaI GGTCTC 1 cut(s) 1574
BsaJI CCNNGG 4 cut(s) 393, 1125, 1509, 1605
BsaWI WCCGGW 1 cut(s) 354
BsaXI ACNNNNNCTCC 4 cut(s) 105, 135, 275, 305
Bse118I RCCGGY 2 cut(s) 527, 1222
Bse1I ACTGG 1 cut(s) 790
Bse21I CCTNAGG 1 cut(s) 872
Bse3DI GCAATG 2 cut(s) 76, 1526
BseBI CCWGG 2 cut(s) 336, 601
BseDI CCNNGG 4 cut(s) 393, 1125, 1509, 1605
BseGI GGATG 3 cut(s) 559, 860, 1077
BseMI GCAATG 2 cut(s) 76, 1526
BseNI ACTGG 1 cut(s) 790
BseRI GAGGAG 1 cut(s) 296
BseXI GCAGC 4 cut(s) 869, 1056, 1518, 1574
BsgI GTGCAG 1 cut(s) 822
Bsh1236I CGCG 2 cut(s) 44, 452
BshFI GGCC 5 cut(s) 392, 512, 522, 968, 1256
BshNI GGYRCC 1 cut(s) 1121
BsiHKAI GWGCWC 2 cut(s) 716, 785
BsiHKCI CYCGRG 2 cut(s) 593, 1484
BsiSI CCGG 5 cut(s) 355, 528, 1152, 1223, 1511
BslFI GGGAC 3 cut(s) 181, 1213, 1313
BsmAI GTCTC 5 cut(s) 80, 651, 944, 1091, 1574
BsmBI CGTCTC 1 cut(s) 80
BsmFI GGGAC 3 cut(s) 181, 1213, 1313
BsnI GGCC 5 cut(s) 392, 512, 522, 968, 1256
Bso31I GGTCTC 1 cut(s) 1574
BsoBI CYCGRG 2 cut(s) 593, 1484
Bsp1286I GDGCHC 2 cut(s) 716, 785
Bsp1407I TGTACA 1 cut(s) 305
Bsp143I GATC 9 cut(s) 6, 35, 286, 459, 619, 639, 957, 1449, 1496
Bsp19I CCATGG 1 cut(s) 393
BspACI CCGC 8 cut(s) 243, 450, 697, 740, 1092, 1119, 1134, 1297
BspANI GGCC 5 cut(s) 392, 512, 522, 968, 1256
BspFNI CGCG 2 cut(s) 44, 452
BspLI GGNNCC 3 cut(s) 823, 1123, 1156
BspMI ACCTGC 1 cut(s) 120
BspPI GGATC 4 cut(s) 294, 467, 647, 1504
BspQI GCTCTTC 1 cut(s) 876
BspT107I GGYRCC 1 cut(s) 1121
BspTI CTTAAG 1 cut(s) 539
BspTNI GGTCTC 1 cut(s) 1574
BsrBI CCGCTC 1 cut(s) 243
BsrDI GCAATG 2 cut(s) 76, 1526
BsrFI RCCGGY 2 cut(s) 527, 1222
BsrGI TGTACA 1 cut(s) 305
BsrI ACTGG 1 cut(s) 790
BssAI RCCGGY 2 cut(s) 527, 1222
BssECI CCNNGG 4 cut(s) 393, 1125, 1509, 1605
BssMI GATC 9 cut(s) 6, 35, 286, 459, 619, 639, 957, 1449, 1496
BssSI CACGAG 1 cut(s) 1066
BssT1I CCWWGG 2 cut(s) 393, 1605
Bst2BI CACGAG 1 cut(s) 1066
Bst2UI CCWGG 2 cut(s) 336, 601
Bst6I CTCTTC 4 cut(s) 393, 876, 1237, 1536
BstAFI CTTAAG 1 cut(s) 539
BstAUI TGTACA 1 cut(s) 305
BstBAI YACGTR 2 cut(s) 1050, 1384
BstC8I GCNNGC 3 cut(s) 970, 976, 982
BstDEI CTNAG 2 cut(s) 872, 1260
BstDSI CCRYGG 1 cut(s) 393
BstEII GGTNACC 1 cut(s) 1129
BstF5I GGATG 3 cut(s) 559, 860, 1077
BstFNI CGCG 2 cut(s) 44, 452
BstH2I RGCGCY 1 cut(s) 981
BstHHI GCGC 3 cut(s) 800, 974, 980
BstKTI GATC 9 cut(s) 9, 38, 289, 462, 622, 642, 960, 1452, 1499
BstMAI GTCTC 5 cut(s) 80, 651, 944, 1091, 1574
BstMBI GATC 9 cut(s) 6, 35, 286, 459, 619, 639, 957, 1449, 1496
BstMWI GCNNNNNNNGC 3 cut(s) 528, 883, 1303
BstNI CCWGG 2 cut(s) 336, 601
BstNSI RCATGY 2 cut(s) 509, 1538
BstPI GGTNACC 1 cut(s) 1129
BstSCI CCNGG 4 cut(s) 334, 599, 1151, 1509
BstSFI CTRYAG 1 cut(s) 64
BstUI CGCG 2 cut(s) 44, 452
BstV1I GCAGC 4 cut(s) 869, 1056, 1518, 1574
BstV2I GAAGAC 1 cut(s) 1553
BstX2I RGATCY 2 cut(s) 35, 459
BstXI CCANNNNNNTGG 1 cut(s) 1595
BstYI RGATCY 2 cut(s) 35, 459
Bsu36I CCTNAGG 1 cut(s) 872
BsuRI GGCC 5 cut(s) 392, 512, 522, 968, 1256
BtgI CCRYGG 1 cut(s) 393
BtgZI GCGATG 1 cut(s) 838
BtrI CACGTC 1 cut(s) 1303
BtsCI GGATG 3 cut(s) 559, 860, 1077
BtsIMutI CAGTG 2 cut(s) 308, 615
BveI ACCTGC 1 cut(s) 120
Cac8I GCNNGC 3 cut(s) 970, 976, 982
CfoI GCGC 3 cut(s) 800, 974, 980
Cfr10I RCCGGY 2 cut(s) 527, 1222
Cfr13I GGNCC 3 cut(s) 352, 787, 1155
CseI GACGC 2 cut(s) 97, 275
Csp6I GTAC 8 cut(s) 179, 231, 306, 404, 604, 1027, 1122, 1220
CspCI CAANNNNNGTGG 2 cut(s) 489, 524
CviQI GTAC 8 cut(s) 179, 231, 306, 404, 604, 1027, 1122, 1220
DdeI CTNAG 2 cut(s) 872, 1260
DpnI GATC 9 cut(s) 8, 37, 288, 461, 621, 641, 959, 1451, 1498
DpnII GATC 9 cut(s) 6, 35, 286, 459, 619, 639, 957, 1449, 1496
DraIII CACNNNGTG 1 cut(s) 1384
DriI GACNNNNNGTC 1 cut(s) 481
EaeI YGGCCR 3 cut(s) 390, 966, 1254
Eam1104I CTCTTC 4 cut(s) 393, 876, 1237, 1536
Eam1105I GACNNNNNGTC 1 cut(s) 481
EarI CTCTTC 4 cut(s) 393, 876, 1237, 1536
Eco130I CCWWGG 2 cut(s) 393, 1605
Eco31I GGTCTC 1 cut(s) 1574
Eco47I GGWCC 3 cut(s) 352, 787, 1155
Eco57I CTGAAG 3 cut(s) 456, 978, 1315
Eco72I CACGTG 1 cut(s) 1050
Eco81I CCTNAGG 1 cut(s) 872
Eco88I CYCGRG 2 cut(s) 593, 1484
Eco91I GGTNACC 1 cut(s) 1129
EcoO109I RGGNCCY 1 cut(s) 1155
EcoO65I GGTNACC 1 cut(s) 1129
EcoRI GAATTC 1 cut(s) 1097
EcoRII CCWGG 2 cut(s) 334, 599
EcoT14I CCWWGG 2 cut(s) 393, 1605
EcoT22I ATGCAT 1 cut(s) 817
ErhI CCWWGG 2 cut(s) 393, 1605
Esp3I CGTCTC 1 cut(s) 80
FaqI GGGAC 3 cut(s) 181, 1213, 1313
FauI CCCGC 2 cut(s) 704, 1290
Fnu4HI GCNGC 4 cut(s) 858, 1045, 1532, 1563
FokI GGATG 3 cut(s) 566, 847, 1084
Fsp4HI GCNGC 4 cut(s) 858, 1045, 1532, 1563
FspBI CTAG 4 cut(s) 176, 321, 897, 1517
GlaI GCGC 3 cut(s) 799, 973, 979
GluI GCNGC 4 cut(s) 858, 1045, 1532, 1563
HaeII RGCGCY 1 cut(s) 981
HaeIII GGCC 5 cut(s) 392, 512, 522, 968, 1256
HapII CCGG 5 cut(s) 355, 528, 1152, 1223, 1511
HgaI GACGC 2 cut(s) 97, 275
HhaI GCGC 3 cut(s) 800, 974, 980
Hin6I GCGC 3 cut(s) 798, 972, 978
HinP1I GCGC 3 cut(s) 798, 972, 978
HindIII AAGCTT 1 cut(s) 542
HpaII CCGG 5 cut(s) 355, 528, 1152, 1223, 1511
HphI GGTGA 8 cut(s) 257, 502, 754, 760, 838, 1081, 1093, 1123
Hpy166II GTNNAC 6 cut(s) 109, 306, 308, 613, 751, 1353
Hpy188I TCNGA 6 cut(s) 79, 85, 957, 1041, 1186, 1411
Hpy188III TCNNGA 4 cut(s) 263, 593, 1333, 1439
Hpy8I GTNNAC 6 cut(s) 109, 306, 308, 613, 751, 1353
Hpy99I CGWCG 2 cut(s) 1277, 1411
HpyAV CCTTC 5 cut(s) 210, 523, 624, 733, 739
HpyCH4IV ACGT 5 cut(s) 728, 1049, 1275, 1302, 1383
HpyCH4V TGCA 7 cut(s) 332, 386, 557, 815, 839, 1534, 1562
HpyF10VI GCNNNNNNNGC 3 cut(s) 528, 883, 1303
HpyF3I CTNAG 2 cut(s) 872, 1260
HpySE526I ACGT 5 cut(s) 728, 1049, 1275, 1302, 1383
HspAI GCGC 3 cut(s) 798, 972, 978
KpnI GGTACC 1 cut(s) 1125
Kzo9I GATC 9 cut(s) 6, 35, 286, 459, 619, 639, 957, 1449, 1496
LguI GCTCTTC 1 cut(s) 876
LmnI GCTCC 1 cut(s) 240
Lsp1109I GCAGC 4 cut(s) 869, 1056, 1518, 1574
LweI GCATC 5 cut(s) 544, 861, 869, 1368, 1549
MaeI CTAG 4 cut(s) 176, 321, 897, 1517
MaeII ACGT 5 cut(s) 728, 1049, 1275, 1302, 1383
MaeIII GTNAC 4 cut(s) 12, 194, 1129, 1387
MalI GATC 9 cut(s) 8, 37, 288, 461, 621, 641, 959, 1451, 1498
MbiI CCGCTC 1 cut(s) 243
MboI GATC 9 cut(s) 6, 35, 286, 459, 619, 639, 957, 1449, 1496
MboII GAAGA 7 cut(s) 410, 438, 893, 1224, 1321, 1553, 1558
MfeI CAATTG 1 cut(s) 24
MflI RGATCY 2 cut(s) 35, 459
MhlI GDGCHC 2 cut(s) 716, 785
MlsI TGGCCA 2 cut(s) 968, 1256
MluCI AATT 6 cut(s) 24, 117, 222, 532, 1013, 1097
MluI ACGCGT 1 cut(s) 42
MluNI TGGCCA 2 cut(s) 968, 1256
MlyI GAGTC 5 cut(s) 491, 605, 946, 1045, 1093
MmeI TCCRAC 3 cut(s) 437, 1389, 1576
Mox20I TGGCCA 2 cut(s) 968, 1256
Mph1103I ATGCAT 1 cut(s) 817
MroXI GAANNNNTTC 1 cut(s) 683
MscI TGGCCA 2 cut(s) 968, 1256
MseI TTAA 2 cut(s) 431, 540
MslI CAYNNNNRTG 1 cut(s) 669
Msp20I TGGCCA 2 cut(s) 968, 1256
MspA1I CMGCKG 2 cut(s) 1136, 1297
MspCI CTTAAG 1 cut(s) 539
MspI CCGG 5 cut(s) 355, 528, 1152, 1223, 1511
MspR9I CCNGG 4 cut(s) 336, 601, 1153, 1511
MunI CAATTG 1 cut(s) 24
MvaI CCWGG 2 cut(s) 336, 601
MvnI CGCG 2 cut(s) 44, 452
MwoI GCNNNNNNNGC 3 cut(s) 528, 883, 1303
NciI CCSGG 2 cut(s) 1153, 1511
NcoI CCATGG 1 cut(s) 393
NdeII GATC 9 cut(s) 6, 35, 286, 459, 619, 639, 957, 1449, 1496
NlaIV GGNNCC 3 cut(s) 823, 1123, 1156
NmuCI GTSAC 2 cut(s) 194, 1129
NsiI ATGCAT 1 cut(s) 817
NspI RCATGY 2 cut(s) 509, 1538
PciSI GCTCTTC 1 cut(s) 876
PcsI WCGNNNNNNNCGW 7 cut(s) 933, 1055, 1064, 1122, 1320, 1403, 1458
PdmI GAANNNNTTC 1 cut(s) 683
PfeI GAWTC 5 cut(s) 74, 80, 903, 1412, 1601
PflMI CCANNNNNTGG 2 cut(s) 817, 1384
PkrI GCNGC 4 cut(s) 859, 1046, 1533, 1564
PleI GAGTC 5 cut(s) 490, 604, 946, 1044, 1092
PmaCI CACGTG 1 cut(s) 1050
PmlI CACGTG 1 cut(s) 1050
PpsI GAGTC 5 cut(s) 490, 604, 946, 1044, 1092
Ppu21I YACGTR 2 cut(s) 1050, 1384
PpuMI RGGWCCY 1 cut(s) 1155
Psp5II RGGWCCY 1 cut(s) 1155
Psp6I CCWGG 2 cut(s) 334, 599
PspCI CACGTG 1 cut(s) 1050
PspEI GGTNACC 1 cut(s) 1129
PspGI CCWGG 2 cut(s) 334, 599
PspN4I GGNNCC 3 cut(s) 823, 1123, 1156
PspPI GGNCC 3 cut(s) 352, 787, 1155
PspPPI RGGWCCY 1 cut(s) 1155
PsrI GAACNNNNNNTAC 2 cut(s) 1162, 1194
PsuI RGATCY 2 cut(s) 35, 459
RsaI GTAC 8 cut(s) 180, 232, 307, 405, 605, 1028, 1123, 1221
RsaNI GTAC 8 cut(s) 179, 231, 306, 404, 604, 1027, 1122, 1220
RseI CAYNNNNRTG 1 cut(s) 669
SapI GCTCTTC 1 cut(s) 876
SaqAI TTAA 2 cut(s) 431, 540
SatI GCNGC 4 cut(s) 858, 1045, 1532, 1563
Sau3AI GATC 9 cut(s) 6, 35, 286, 459, 619, 639, 957, 1449, 1496
Sau96I GGNCC 3 cut(s) 352, 787, 1155
SchI GAGTC 5 cut(s) 491, 605, 946, 1045, 1093
ScrFI CCNGG 4 cut(s) 336, 601, 1153, 1511
SduI GDGCHC 2 cut(s) 716, 785
SfaNI GCATC 5 cut(s) 544, 861, 869, 1368, 1549
SfcI CTRYAG 1 cut(s) 64
SinI GGWCC 3 cut(s) 352, 787, 1155
SmiMI CAYNNNNRTG 1 cut(s) 669
SmlI CTYRAG 2 cut(s) 58, 539
SmoI CTYRAG 2 cut(s) 58, 539
Sse9I AATT 6 cut(s) 24, 117, 222, 532, 1013, 1097
SsiI CCGC 8 cut(s) 243, 450, 697, 740, 1092, 1119, 1134, 1297
SspMI CTAG 4 cut(s) 176, 321, 897, 1517
StyD4I CCNGG 4 cut(s) 334, 599, 1151, 1509
StyI CCWWGG 2 cut(s) 393, 1605
TaiI ACGT 5 cut(s) 731, 1052, 1278, 1305, 1386
TaqI TCGA 6 cut(s) 5, 48, 921, 1023, 1058, 1452
TasI AATT 6 cut(s) 24, 117, 222, 532, 1013, 1097
TatI WGTACW 3 cut(s) 178, 305, 1026
TfiI GAWTC 5 cut(s) 74, 80, 903, 1412, 1601
Tru1I TTAA 2 cut(s) 431, 540
Tru9I TTAA 2 cut(s) 431, 540
TscAI CASTG 2 cut(s) 315, 622
TseFI GTSAC 2 cut(s) 194, 1129
TseI GCWGC 4 cut(s) 857, 1044, 1531, 1562
Tsp45I GTSAC 2 cut(s) 194, 1129
TspDTI ATGAA 6 cut(s) 288, 431, 559, 676, 692, 1102
TspGWI ACGGA 4 cut(s) 126, 642, 800, 1098
TspRI CASTG 2 cut(s) 315, 622
Van91I CCANNNNNTGG 2 cut(s) 817, 1384
Vha464I CTTAAG 1 cut(s) 539
VpaK11BI GGWCC 3 cut(s) 352, 787, 1155
XapI RAATTY 1 cut(s) 1097
XceI RCATGY 2 cut(s) 509, 1538
XmnI GAANNNNTTC 1 cut(s) 683
XspI CTAG 4 cut(s) 176, 321, 897, 1517
Zsp2I ATGCAT 1 cut(s) 817
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.