Rh2CG552800

Agglutinin domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0000217
Physical Location & Seq
Forward (+)
73207326 .. 73218928
11603 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG552800.1

Sequence Viewer

Length: 1659 bp
ATGCCGGAAAACCCAGTTGAAGTGATCAAAGATATGCTGGTATTAGTAAGATCAACTGAAGAAGAGAAAATGTCTTTCTTATTACCACCTACTATTGTTCTGAAATCATCGTGGTTCAACACTTACTTGAACTACAACAATGATTCTGATTCCGAGACACATGGCTTTCTCAAAGTCAACGGATCGAATGTCTTCAGCCCATTAGCAAAGTTCGAAGTGGTGACGGCCAATACTGGGACGGGACTGGTACATATCAGATGTTCTCAGAACAGAAAGTTCCTGCGAAGGAAATTTGAGAGTGATGTGAACCTTACTCCGGAGGCTGATGAACCAGAAGAGGACCAATCCAAATGGTCGTGTACACTATTTGAGCCTCAGGTAGTTGATGGTAACCCTGACCAAGCCCGGTTGTTCCATGTCCAAGCAAAGCTATATGCAACGGCATGGAACGAAGGTGGAGGCGAAAACAAAAAGTTCTTCAGATTACATGTTGCTACGAGTGTTCAGGGTACCTTTGATATCATTGACTTGGAGTCATTGGTGATATTGCCAAAGCATGTGGCCTTCAAAGGTCACGCCGGCAATTACCTTCAGCTTGTGGCGGATGCTAAGCTCAAGTTCATATCCACCGATAAGGGGATTCCTGAGTCCTGGTTTGAGGTGTCCACTGATGGCGACGGAATGGTGCAAATCAAATCCTACCACGACAATAGCTACTTTTTTAACAGCAGTGACAGGATTTGGTCTGACCCAAAGGTTGCCGCCACCGATCCAAGCACCTTGTTTTGGCCGGTGAAGGTCGACAGTAATAAGGTCGCTTTCAAAAGCAACAACAAATTTTGTGCCCAAATCAGTGATGCTAATGCAACAGACGGTCTGAAGGCAGCCTACACCAGCATTCCAACAACCTCACGCTTCACGCTGGAGGAGCTTGTTCTCTCTAGGAGAATTTACAACACCGACTTCGATCTTAAGAATGCCATAATCTACGGTGAGACCCCGATCACCATGGCCACCGCAAATGCCAGCAACAACACCTCAGTAGACAACACTGTGGAATTCAAGTTCGCGTACACGGAGAGTAAGAGCAGCACGTGGAGCGCCAGCCACTCGTGGTCGGTAGGCGTGAGTGTCACCGCCAGCTTCAAAGTTCCATTCATCGGCGGTACTGACGTCACTGCTTCAGCAGAGTATTCCGGATCTTATGAGTGGGGGGAAACTGTTACGTCAGAAAACACATTGGAAACTACCTACACGGTCACAGTGCCGGCAAACACTTCTATATCTGTGAGCCTGATGGCGACTAAGGGAACTTGCGACGTTCCTTACTCTTATTATCAGCGGGACGTGCTTTATAACGGTAAAACCGTTGTCTATAAGAAAGATGACGGGCTGTACACGGGTGTTAATTCATACAACTTCCGCTATGAGGTTACAACACTGAAAGAGCCAGAAACTAGAGCTGATTTGCCAACCAGACAGCAGAAGTTGCCGGACCCATCGGTCGACCCCTCCATAAAACAAAAGCTACCTCTGCCCGTGCCGTCTGAATCAACCACCAGAGTGCAAAACAAGGTGACCTCGACCACCACCCAGGAACAAGAGTTGTCAATGCTGGGATTAGAAAATGCAGCAGCTCGATCTGCCGTGAAACCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aerolysin Agglutinin ETX_MTX2 Fascin

Protein Analysis

552

Amino Acids

61.36

Weight (kDa)

5.28

Isoelectric Point (pI)

32.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Agglutinin PF07468 28 - 157 3.3e-18 Agglutinin domain
Aerolysin PF01117 324 - 445 1.6e-09 Aerolysin toxin
ETX_MTX2 PF03318 332 - 444 8.3e-09 Clostridium epsilon toxin ETX/Bacillus mosquitocidal toxin MTX2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000217)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g17710 FvH4_6g45590 FvH4_6g45591 FvH4_6g45770 FvH4_6g45770 FvH4_6g46030 FvH4_6g46040 FvH4_7g07020 FvH4_7g07071 FvH4_7g07080
malus_domestica MD01G1104300.v1.1 MD02G1240000.v1.1 MD02G1240200.v1.1 MD03G1201400.v1.1 MD03G1201500.v1.1 MD07G1171100.v1.1
prunus_persica Prupe.2G087400_v2.0.a1 Prupe.2G097500_v2.0.a1 Prupe.2G097700_v2.0.a1 Prupe.2G097800_v2.0.a1 Prupe.3G153100_v2.0.a1 Prupe.3G153300_v2.0.a1 Prupe.3G153600_v2.0.a1 Prupe.5G237200_v2.0.a1
pyrus_communis pycom03g15270 pycom06g20700 pycom07g06030 pycom10g01810 pycom10g01820 pycom10g12090 pycom14g20090 pycom14g20100 pycom14g20110 pycom14g20120
rosa_chinensis RchiOBHm_Chr1g0336971 RchiOBHm_Chr1g0337031 RchiOBHm_Chr2g0163571 RchiOBHm_Chr2g0163591 RchiOBHm_Chr2g0163631 RchiOBHm_Chr2g0163661 RchiOBHm_Chr2g0163671 RchiOBHm_Chr2g0163691 RchiOBHm_Chr2g0163741 RchiOBHm_Chr5g0011821 RchiOBHm_Chr5g0011831 RchiOBHm_Chr5g0011841 RchiOBHm_Chr5g0053101 RchiOBHm_Chr5g0072501
rosa_laevigata RLG00000010009 RLG00000021413 RLG00000029310 RLG00000029318 RLG00000036313
rosa_multiflora Rmu_co7975848.1_g000001 Rmu_co8020886.1_g000001 Rmu_co8136580.1_g000001 Rmu_co8177372.1_g000001 Rmu_co8207014.1_g000001 Rmu_co8232187.1_g000001 Rmu_co8277595.1_g000001 Rmu_co8389219.1_g000001 Rmu_co8427877.1_g000001 Rmu_co8467587.1_g000001 Rmu_sc0001475.1_g000015 Rmu_sc0001475.1_g000016 Rmu_sc0001475.1_g000017 Rmu_sc0003462.1_g000002 Rmu_sc0003462.1_g000003 Rmu_sc0003484.1_g000002 Rmu_sc0004918.1_g000004 Rmu_sc0004918.1_g000006 Rmu_sc0004918.1_g000007 Rmu_sc0004918.1_g000009 Rmu_sc0006596.1_g000003 Rmu_sc0006596.1_g000005 Rmu_sc0006596.1_g000010 Rmu_sc0008676.1_g000021 Rmu_sc0014330.1_g000002 Rmu_sc0016133.1_g000002 Rmu_sc0016133.1_g000003 Rmu_sc0016133.1_g000004 Rmu_sc0026453.1_g000003 Rmu_sc0026453.1_g000004 Rmu_sc0030660.1_g000001
rosa_roxburghii Rroxscaffold_1G00008700 Rroxscaffold_1G00064740 Rroxscaffold_2G00086950 Rroxscaffold_2G00087100 Rroxscaffold_2G00087140 Rroxscaffold_4G00302080 Rroxscaffold_4G00315020 Rroxscaffold_4G00315170 Rroxscaffold_5G00358800
rosa_rugosa Rorug01G0129200 Rorug01G0130600 Rorug01G0130700 Rorug02G0503700 Rorug02G0503800 Rorug02G0503900 Rorug02G0504000 Rorug02G0504300 Rorug05G0268200 Rorug05G0418700 Rorug05G0418800
rosa_samantha Rh1AG151100 Rh1AG151300 Rh1BG118400 Rh1BG120600 Rh1CG141500 Rh1CG142500 Rh1DG157300 Rh1DG158500 Rh2AG570600 Rh2AG570800 Rh2AG571200 Rh2AG571300 Rh2AG571500 Rh2AG571900 Rh2AG572000 Rh2AG572200 Rh2CG552600 Rh2CG552800 Rh2CG553000 Rh2CG553300 Rh2CG553400 Rh2CG553700 Rh5CG100400 Rh5CG100500 Rh5CG100600 Rh5CG100700 Rh5CG519100 Rh5DG086600 Rh5DG086700 Rh5DG086800 Rh5DG086900 Rh5DG087000 Rh5DG087100 Rh5DG367600 Rh5DG507300
rosa_wichuraiana Rw1G012130 Rw1G012240 Rw1G012380 Rw2G047240 Rw2G047260 Rw2G047270 Rw2G047290 Rw5G007940 Rw5G044120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1356
AasI GACNNNNNNGTC 1 cut(s) 1502
AatII GACGTC 1 cut(s) 1176
Acc65I GGTACC 1 cut(s) 509
AccB1I GGYRCC 1 cut(s) 509
AccI GTMKAC 3 cut(s) 801, 1044, 1506
AccII CGCG 1 cut(s) 1070
AccIII TCCGGA 2 cut(s) 316, 1196
AciI CCGC 7 cut(s) 602, 762, 1017, 1137, 1164, 1342, 1423
AclWI GGATC 3 cut(s) 190, 764, 1207
AcoI YGGCCR 3 cut(s) 225, 788, 1011
AcsI RAATTY 4 cut(s) 290, 836, 948, 1058
AcuI CTGAAG 6 cut(s) 78, 178, 463, 575, 899, 1167
AcvI CACGTG 1 cut(s) 1095
AcyI GRCGYC 1 cut(s) 1173
AfaI GTAC 6 cut(s) 249, 361, 511, 1073, 1168, 1397
AfiI CCNNNNNNNGG 6 cut(s) 234, 316, 636, 786, 1160, 1429
AflII CTTAAG 1 cut(s) 971
AflIII ACRYGT 1 cut(s) 487
AgsI TTSAA 7 cut(s) 20, 118, 130, 568, 823, 1063, 1147
AhdI GACNNNNNGTC 1 cut(s) 532
AjiI CACGTC 1 cut(s) 1348
AjnI CCWGG 2 cut(s) 650, 1593
AleI CACNNNNGTG 1 cut(s) 1562
AloI GAACNNNNNNTCC 2 cut(s) 1049, 1081
AluBI AGCT 9 cut(s) 430, 595, 613, 714, 931, 1143, 1463, 1528, 1637
AluI AGCT 9 cut(s) 430, 595, 613, 714, 931, 1143, 1463, 1528, 1637
Alw26I GTCTC 2 cut(s) 149, 989
AlwI GGATC 3 cut(s) 190, 764, 1207
Aor13HI TCCGGA 2 cut(s) 316, 1196
AoxI GGCC 4 cut(s) 225, 561, 788, 1011
ApeKI GCWGC 4 cut(s) 884, 1089, 1631, 1634
ApoI RAATTY 4 cut(s) 290, 836, 948, 1058
Asp700I GAANNNNTTC 1 cut(s) 191
Asp718I GGTACC 1 cut(s) 509
AspLEI GCGC 1 cut(s) 1103
AspS9I GGNCC 2 cut(s) 340, 1495
AsuC2I CCSGG 1 cut(s) 406
AsuHPI GGTGA 7 cut(s) 232, 553, 805, 997, 1004, 1126, 1588
AsuII TTCGAA 1 cut(s) 213
AvaII GGWCC 2 cut(s) 340, 1495
AxyI CCTNAGG 1 cut(s) 375
BaeGI GKGCMC 1 cut(s) 847
BaeI ACNNNNGTAYC 2 cut(s) 1158, 1191
BalI TGGCCA 1 cut(s) 1013
BanI GGYRCC 1 cut(s) 509
BarI GAAGNNNNNNTAC 2 cut(s) 872, 904
BauI CACGAG 1 cut(s) 1111
BbrPI CACGTG 1 cut(s) 1095
BbsI GAAGAC 1 cut(s) 184
BbvI GCAGC 4 cut(s) 896, 1101, 1643, 1646
BccI CCATC 4 cut(s) 380, 665, 1291, 1507
BceAI ACGGC 4 cut(s) 240, 456, 1528, 1631
BciT130I CCWGG 2 cut(s) 652, 1595
BclI TGATCA 1 cut(s) 24
BcnI CCSGG 1 cut(s) 406
BcoDI GTCTC 2 cut(s) 149, 989
BfaI CTAG 3 cut(s) 942, 1458, 1657
BfoI RGCGCY 1 cut(s) 1104
BfrI CTTAAG 1 cut(s) 971
BisI GCNGC 5 cut(s) 762, 885, 1090, 1632, 1635
BlpI GCTNAGC 1 cut(s) 609
BlsI GCNGC 5 cut(s) 763, 886, 1091, 1633, 1636
Bme1390I CCNGG 3 cut(s) 406, 652, 1595
Bme18I GGWCC 2 cut(s) 340, 1495
BmeRI GACNNNNNGTC 1 cut(s) 532
BmgBI CACGTC 1 cut(s) 1348
BmgT120I GGNCC 2 cut(s) 340, 1495
BmiI GGNNCC 2 cut(s) 511, 1497
BmrFI CCNGG 3 cut(s) 406, 652, 1595
BmrI ACTGGG 2 cut(s) 8, 243
BmsI GCATC 2 cut(s) 595, 847
BmuI ACTGGG 2 cut(s) 8, 243
BpiI GAAGAC 1 cut(s) 184
BpmI CTGGAG 1 cut(s) 944
Bpu1102I GCTNAGC 1 cut(s) 609
Bpu14I TTCGAA 1 cut(s) 213
BpuEI CTTGAG 1 cut(s) 599
BpuMI CCSGG 1 cut(s) 406
BsaAI YACGTR 1 cut(s) 1095
BsaHI GRCGYC 1 cut(s) 1173
BsaI GGTCTC 1 cut(s) 989
BsaJI CCNNGG 2 cut(s) 1008, 1593
BsaWI WCCGGW 2 cut(s) 316, 1196
Bsc4I CCNNNNNNNGG 6 cut(s) 234, 316, 636, 786, 1160, 1429
Bse118I RCCGGY 3 cut(s) 578, 790, 1267
Bse1I ACTGG 3 cut(s) 14, 238, 249
Bse21I CCTNAGG 1 cut(s) 375
BseAI TCCGGA 2 cut(s) 316, 1196
BseBI CCWGG 2 cut(s) 652, 1595
BseDI CCNNGG 2 cut(s) 1008, 1593
BseGI GGATG 1 cut(s) 610
BseLI CCNNNNNNNGG 6 cut(s) 234, 316, 636, 786, 1160, 1429
BseMII CTCAG 4 cut(s) 278, 389, 636, 1053
BseNI ACTGG 3 cut(s) 14, 238, 249
BseRI GAGGAG 1 cut(s) 941
BseSI GKGCMC 1 cut(s) 847
BseXI GCAGC 4 cut(s) 896, 1101, 1643, 1646
BseYI CCCAGC 1 cut(s) 1615
Bsh1236I CGCG 1 cut(s) 1070
Bsh1285I CGRYCG 1 cut(s) 1506
BshFI GGCC 4 cut(s) 227, 563, 790, 1013
BshNI GGYRCC 1 cut(s) 509
BsiEI CGRYCG 1 cut(s) 1506
BsiSI CCGG 8 cut(s) 5, 317, 406, 579, 791, 1197, 1268, 1493
BslFI GGGAC 3 cut(s) 250, 255, 1358
BslI CCNNNNNNNGG 6 cut(s) 234, 316, 636, 786, 1160, 1429
BsmAI GTCTC 2 cut(s) 149, 989
BsmFI GGGAC 3 cut(s) 250, 255, 1358
BsmI GAATGC 2 cut(s) 897, 982
BsnI GGCC 4 cut(s) 227, 563, 790, 1013
Bso31I GGTCTC 1 cut(s) 989
Bsp119I TTCGAA 1 cut(s) 213
Bsp1286I GDGCHC 1 cut(s) 847
Bsp13I TCCGGA 2 cut(s) 316, 1196
Bsp1407I TGTACA 2 cut(s) 359, 1395
Bsp143I GATC 8 cut(s) 24, 50, 182, 769, 967, 1002, 1199, 1640
Bsp1720I GCTNAGC 1 cut(s) 609
Bsp19I CCATGG 1 cut(s) 1008
BspACI CCGC 7 cut(s) 602, 762, 1017, 1137, 1164, 1342, 1423
BspANI GGCC 4 cut(s) 227, 563, 790, 1013
BspCNI CTCAG 4 cut(s) 277, 388, 637, 1052
BspEI TCCGGA 2 cut(s) 316, 1196
BspFNI CGCG 1 cut(s) 1070
BspLI GGNNCC 2 cut(s) 511, 1497
BspPI GGATC 3 cut(s) 190, 764, 1207
BspT104I TTCGAA 1 cut(s) 213
BspT107I GGYRCC 1 cut(s) 509
BspTI CTTAAG 1 cut(s) 971
BspTNI GGTCTC 1 cut(s) 989
BsrFI RCCGGY 3 cut(s) 578, 790, 1267
BsrGI TGTACA 2 cut(s) 359, 1395
BsrI ACTGG 3 cut(s) 14, 238, 249
BssAI RCCGGY 3 cut(s) 578, 790, 1267
BssECI CCNNGG 2 cut(s) 1008, 1593
BssMI GATC 8 cut(s) 24, 50, 182, 769, 967, 1002, 1199, 1640
BssNI GRCGYC 1 cut(s) 1173
BssSI CACGAG 1 cut(s) 1111
BssT1I CCWWGG 1 cut(s) 1008
Bst2BI CACGAG 1 cut(s) 1111
Bst2UI CCWGG 2 cut(s) 652, 1595
Bst4CI ACNGT 9 cut(s) 806, 875, 992, 1054, 1222, 1258, 1264, 1361, 1369
Bst6I CTCTTC 2 cut(s) 57, 330
BstACI GRCGYC 1 cut(s) 1173
BstAFI CTTAAG 1 cut(s) 971
BstAPI GCANNNNNTGC 1 cut(s) 1489
BstAUI TGTACA 2 cut(s) 359, 1395
BstBAI YACGTR 1 cut(s) 1095
BstBI TTCGAA 1 cut(s) 213
BstC8I GCNNGC 5 cut(s) 580, 1027, 1105, 1141, 1269
BstDEI CTNAG 6 cut(s) 264, 375, 609, 645, 1039, 1305
BstDSI CCRYGG 1 cut(s) 1008
BstEII GGTNACC 2 cut(s) 389, 1576
BstF5I GGATG 1 cut(s) 610
BstFNI CGCG 1 cut(s) 1070
BstH2I RGCGCY 1 cut(s) 1104
BstHHI GCGC 1 cut(s) 1103
BstKTI GATC 8 cut(s) 27, 53, 185, 772, 970, 1005, 1202, 1643
BstMAI GTCTC 2 cut(s) 149, 989
BstMBI GATC 8 cut(s) 24, 50, 182, 769, 967, 1002, 1199, 1640
BstMCI CGRYCG 1 cut(s) 1506
BstMWI GCNNNNNNNGC 6 cut(s) 928, 1098, 1348, 1489, 1534, 1643
BstNI CCWGG 2 cut(s) 652, 1595
BstNSI RCATGY 2 cut(s) 491, 560
BstPI GGTNACC 2 cut(s) 389, 1576
BstSCI CCNGG 3 cut(s) 404, 650, 1593
BstSLI GKGCMC 1 cut(s) 847
BstUI CGCG 1 cut(s) 1070
BstV1I GCAGC 4 cut(s) 896, 1101, 1643, 1646
BstV2I GAAGAC 1 cut(s) 184
BstX2I RGATCY 1 cut(s) 1199
BstYI RGATCY 1 cut(s) 1199
Bsu36I CCTNAGG 1 cut(s) 375
BsuRI GGCC 4 cut(s) 227, 563, 790, 1013
BtgI CCRYGG 1 cut(s) 1008
BtrI CACGTC 1 cut(s) 1348
BtsCI GGATG 1 cut(s) 610
BtsI GCAGTG 2 cut(s) 736, 1176
BtsIMutI CAGTG 7 cut(s) 666, 736, 859, 1050, 1176, 1269, 1439
Cac8I GCNNGC 5 cut(s) 580, 1027, 1105, 1141, 1269
CfoI GCGC 1 cut(s) 1103
Cfr10I RCCGGY 3 cut(s) 578, 790, 1267
Cfr13I GGNCC 2 cut(s) 340, 1495
Csp6I GTAC 6 cut(s) 248, 360, 510, 1072, 1167, 1396
CspCI CAANNNNNGTGG 2 cut(s) 540, 575
CviAII CATG 6 cut(s) 161, 416, 444, 488, 557, 1009
CviQI GTAC 6 cut(s) 248, 360, 510, 1072, 1167, 1396
DdeI CTNAG 6 cut(s) 264, 375, 609, 645, 1039, 1305
DpnI GATC 8 cut(s) 26, 52, 184, 771, 969, 1004, 1201, 1642
DpnII GATC 8 cut(s) 24, 50, 182, 769, 967, 1002, 1199, 1640
DrdI GACNNNNNNGTC 1 cut(s) 1502
DriI GACNNNNNGTC 1 cut(s) 532
DseDI GACNNNNNNGTC 1 cut(s) 1502
EaeI YGGCCR 3 cut(s) 225, 788, 1011
Eam1104I CTCTTC 2 cut(s) 57, 330
Eam1105I GACNNNNNGTC 1 cut(s) 532
EarI CTCTTC 2 cut(s) 57, 330
EciI GGCGGA 1 cut(s) 617
Eco130I CCWWGG 1 cut(s) 1008
Eco31I GGTCTC 1 cut(s) 989
Eco32I GATATC 1 cut(s) 520
Eco47I GGWCC 2 cut(s) 340, 1495
Eco57I CTGAAG 6 cut(s) 78, 178, 463, 575, 899, 1167
Eco72I CACGTG 1 cut(s) 1095
Eco81I CCTNAGG 1 cut(s) 375
Eco91I GGTNACC 2 cut(s) 389, 1576
EcoO65I GGTNACC 2 cut(s) 389, 1576
EcoRI GAATTC 1 cut(s) 1058
EcoRII CCWGG 2 cut(s) 650, 1593
EcoRV GATATC 1 cut(s) 520
EcoT14I CCWWGG 1 cut(s) 1008
ErhI CCWWGG 1 cut(s) 1008
FaeI CATG 6 cut(s) 164, 419, 447, 491, 560, 1012
FaqI GGGAC 3 cut(s) 250, 255, 1358
FatI CATG 6 cut(s) 160, 415, 443, 487, 556, 1008
FauI CCCGC 1 cut(s) 1335
FbaI TGATCA 1 cut(s) 24
FblI GTMKAC 3 cut(s) 801, 1044, 1506
Fnu4HI GCNGC 5 cut(s) 762, 885, 1090, 1632, 1635
FokI GGATG 1 cut(s) 617
Fsp4HI GCNGC 5 cut(s) 762, 885, 1090, 1632, 1635
FspBI CTAG 3 cut(s) 942, 1458, 1657
GlaI GCGC 1 cut(s) 1102
GluI GCNGC 5 cut(s) 762, 885, 1090, 1632, 1635
GsaI CCCAGC 1 cut(s) 1619
GsuI CTGGAG 1 cut(s) 944
HaeII RGCGCY 1 cut(s) 1104
HaeIII GGCC 4 cut(s) 227, 563, 790, 1013
HapII CCGG 8 cut(s) 5, 317, 406, 579, 791, 1197, 1268, 1493
HhaI GCGC 1 cut(s) 1103
Hin1I GRCGYC 1 cut(s) 1173
Hin1II CATG 6 cut(s) 164, 419, 447, 491, 560, 1012
Hin6I GCGC 1 cut(s) 1101
HinP1I GCGC 1 cut(s) 1101
HincII GTYRAC 3 cut(s) 178, 802, 1507
HindII GTYRAC 3 cut(s) 178, 802, 1507
HinfI GANTC 6 cut(s) 143, 149, 533, 640, 647, 1550
HpaII CCGG 8 cut(s) 5, 317, 406, 579, 791, 1197, 1268, 1493
HphI GGTGA 7 cut(s) 232, 553, 805, 997, 1004, 1126, 1588
Hpy188III TCNNGA 3 cut(s) 317, 644, 1197
Hpy99I CGWCG 2 cut(s) 680, 1322
HpyAV CCTTC 6 cut(s) 279, 446, 574, 599, 790, 874
HpyCH4III ACNGT 9 cut(s) 806, 875, 992, 1054, 1222, 1258, 1264, 1361, 1369
HpyCH4IV ACGT 5 cut(s) 1094, 1173, 1226, 1320, 1347
HpyCH4V TGCA 5 cut(s) 437, 688, 866, 1567, 1631
HpyF10VI GCNNNNNNNGC 6 cut(s) 928, 1098, 1348, 1489, 1534, 1643
HpyF3I CTNAG 6 cut(s) 264, 375, 609, 645, 1039, 1305
HpySE526I ACGT 5 cut(s) 1094, 1173, 1226, 1320, 1347
Hsp92I GRCGYC 1 cut(s) 1173
Hsp92II CATG 6 cut(s) 164, 419, 447, 491, 560, 1012
HspAI GCGC 1 cut(s) 1101
Kpn2I TCCGGA 2 cut(s) 316, 1196
KpnI GGTACC 1 cut(s) 513
KroI GCCGGC 2 cut(s) 578, 1267
KroNI GCCGGC 2 cut(s) 580, 1269
Ksp22I TGATCA 1 cut(s) 24
Kzo9I GATC 8 cut(s) 24, 50, 182, 769, 967, 1002, 1199, 1640
LmnI GCTCC 2 cut(s) 928, 1098
Lsp1109I GCAGC 4 cut(s) 896, 1101, 1643, 1646
LweI GCATC 2 cut(s) 595, 847
MaeI CTAG 3 cut(s) 942, 1458, 1657
MaeII ACGT 5 cut(s) 1094, 1173, 1226, 1320, 1347
MalI GATC 8 cut(s) 26, 52, 184, 771, 969, 1004, 1201, 1642
MboI GATC 8 cut(s) 24, 50, 182, 769, 967, 1002, 1199, 1640
MboII GAAGA 5 cut(s) 71, 74, 184, 347, 469
MflI RGATCY 1 cut(s) 1199
MhlI GDGCHC 1 cut(s) 847
MlsI TGGCCA 1 cut(s) 1013
MluCI AATT 6 cut(s) 290, 583, 836, 948, 1058, 1408
MluNI TGGCCA 1 cut(s) 1013
MlyI GAGTC 2 cut(s) 542, 656
MmeI TCCRAC 1 cut(s) 926
Mox20I TGGCCA 1 cut(s) 1013
MroI TCCGGA 2 cut(s) 316, 1196
MroNI GCCGGC 2 cut(s) 578, 1267
MroXI GAANNNNTTC 1 cut(s) 191
MscI TGGCCA 1 cut(s) 1013
MseI TTAA 3 cut(s) 723, 972, 1407
MslI CAYNNNNRTG 1 cut(s) 1562
Msp20I TGGCCA 1 cut(s) 1013
MspA1I CMGCKG 1 cut(s) 1342
MspCI CTTAAG 1 cut(s) 971
MspI CCGG 8 cut(s) 5, 317, 406, 579, 791, 1197, 1268, 1493
MspR9I CCNGG 3 cut(s) 406, 652, 1595
Mva1269I GAATGC 2 cut(s) 897, 982
MvaI CCWGG 2 cut(s) 652, 1595
MvnI CGCG 1 cut(s) 1070
MwoI GCNNNNNNNGC 6 cut(s) 928, 1098, 1348, 1489, 1534, 1643
NaeI GCCGGC 2 cut(s) 580, 1269
NciI CCSGG 1 cut(s) 406
NcoI CCATGG 1 cut(s) 1008
NdeII GATC 8 cut(s) 24, 50, 182, 769, 967, 1002, 1199, 1640
NgoMIV GCCGGC 2 cut(s) 578, 1267
NlaIII CATG 6 cut(s) 164, 419, 447, 491, 560, 1012
NlaIV GGNNCC 2 cut(s) 511, 1497
NmuCI GTSAC 7 cut(s) 220, 572, 731, 1132, 1174, 1258, 1576
NspI RCATGY 2 cut(s) 491, 560
NspV TTCGAA 1 cut(s) 213
OliI CACNNNNGTG 1 cut(s) 1562
PciI ACATGT 1 cut(s) 487
PcsI WCGNNNNNNNCGW 1 cut(s) 1365
PctI GAATGC 2 cut(s) 897, 982
PdiI GCCGGC 2 cut(s) 580, 1269
PdmI GAANNNNTTC 1 cut(s) 191
PfeI GAWTC 4 cut(s) 143, 149, 640, 1550
PkrI GCNGC 5 cut(s) 763, 886, 1091, 1633, 1636
PleI GAGTC 2 cut(s) 541, 655
PmaCI CACGTG 1 cut(s) 1095
PmlI CACGTG 1 cut(s) 1095
PpsI GAGTC 2 cut(s) 541, 655
Ppu21I YACGTR 1 cut(s) 1095
PscI ACATGT 1 cut(s) 487
PsiI TTATAA 1 cut(s) 1356
Psp6I CCWGG 2 cut(s) 650, 1593
PspCI CACGTG 1 cut(s) 1095
PspEI GGTNACC 2 cut(s) 389, 1576
PspFI CCCAGC 1 cut(s) 1615
PspGI CCWGG 2 cut(s) 650, 1593
PspN4I GGNNCC 2 cut(s) 511, 1497
PspPI GGNCC 2 cut(s) 340, 1495
PsuI RGATCY 1 cut(s) 1199
RsaI GTAC 6 cut(s) 249, 361, 511, 1073, 1168, 1397
RsaNI GTAC 6 cut(s) 248, 360, 510, 1072, 1167, 1396
RseI CAYNNNNRTG 1 cut(s) 1562
SalI GTCGAC 2 cut(s) 800, 1505
SaqAI TTAA 3 cut(s) 723, 972, 1407
SatI GCNGC 5 cut(s) 762, 885, 1090, 1632, 1635
Sau3AI GATC 8 cut(s) 24, 50, 182, 769, 967, 1002, 1199, 1640
Sau96I GGNCC 2 cut(s) 340, 1495
SchI GAGTC 2 cut(s) 542, 656
ScrFI CCNGG 3 cut(s) 406, 652, 1595
SduI GDGCHC 1 cut(s) 847
SfaNI GCATC 2 cut(s) 595, 847
SfuI TTCGAA 1 cut(s) 213
SinI GGWCC 2 cut(s) 340, 1495
SmiMI CAYNNNNRTG 1 cut(s) 1562
SmlI CTYRAG 2 cut(s) 614, 971
SmoI CTYRAG 2 cut(s) 614, 971
Sse9I AATT 6 cut(s) 290, 583, 836, 948, 1058, 1408
SsiI CCGC 7 cut(s) 602, 762, 1017, 1137, 1164, 1342, 1423
SspMI CTAG 3 cut(s) 942, 1458, 1657
StyD4I CCNGG 3 cut(s) 404, 650, 1593
StyI CCWWGG 1 cut(s) 1008
TaaI ACNGT 9 cut(s) 806, 875, 992, 1054, 1222, 1258, 1264, 1361, 1369
TaiI ACGT 5 cut(s) 1097, 1176, 1229, 1323, 1350
TaqI TCGA 7 cut(s) 185, 213, 801, 966, 1506, 1583, 1639
TaqII GACCGA 1 cut(s) 1492
TasI AATT 6 cut(s) 290, 583, 836, 948, 1058, 1408
TatI WGTACW 2 cut(s) 359, 1395
TauI GCSGC 1 cut(s) 764
TfiI GAWTC 4 cut(s) 143, 149, 640, 1550
Tru1I TTAA 3 cut(s) 723, 972, 1407
Tru9I TTAA 3 cut(s) 723, 972, 1407
TscAI CASTG 7 cut(s) 673, 736, 859, 1057, 1183, 1269, 1446
TseFI GTSAC 7 cut(s) 220, 572, 731, 1132, 1174, 1258, 1576
TseI GCWGC 4 cut(s) 884, 1089, 1631, 1634
Tsp45I GTSAC 7 cut(s) 220, 572, 731, 1132, 1174, 1258, 1576
TspDTI ATGAA 4 cut(s) 342, 610, 1147, 1401
TspGWI ACGGA 3 cut(s) 195, 693, 1091
TspRI CASTG 7 cut(s) 673, 736, 859, 1057, 1183, 1269, 1446
Vha464I CTTAAG 1 cut(s) 971
VpaK11BI GGWCC 2 cut(s) 340, 1495
XapI RAATTY 4 cut(s) 290, 836, 948, 1058
XceI RCATGY 2 cut(s) 491, 560
XmiI GTMKAC 3 cut(s) 801, 1044, 1506
XmnI GAANNNNTTC 1 cut(s) 191
XspI CTAG 3 cut(s) 942, 1458, 1657
ZraI GACGTC 1 cut(s) 1174
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.