Rh5DG507300

Agglutinin domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0000217
Physical Location & Seq
Forward (+)
79257692 .. 79259747
2056 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG507300.1

Sequence Viewer

Length: 1623 bp
ATGTCGGTTCTATTACCTCCCACCATTGCGATCAAAACAGGCTGGGGTACTGCCAACGTCTACATGAACTACAACGATGATTCTGATTCTCAGACTAGGGGCTTCCTCAAATTCAATGGAGGGAATGTTTTCAGCCCGCTAGCAAAGTTCGAAGTGGTGGCCGCAAATACTGGAATAGGAATGGTGCATATAAGATGCTCTCACAATAAGAAGTTCTTGCGAGTGAGGGCCCAGGGCAGTGGGTACATTTCTCCAGACGCTGATGAACCAGAAGAAGACCAATCAAAATGGTCATGTACGTTATTCCAGCCCAGACCTGTAGATGGCAATCCAGACAGCGTTCGGTTGTTCCATGTCCAACTACAGAAATATCCGGTGTTCAGTAACTCTTATTACTTTTGGGTGGGCTATAATAATCCAGCCCAGCAGGATATCCTGGAAATCATTGACCTTGAATCGTTAGTGATACTGCCGAGGCATGTGGCCTTCAAAGGCCATAATGGAAAATATCTCGGAACAGTAGAGCTGAACAACCAACGGTATCTGGCATTCGATTACGCTGCCAAGGGTCGCCCTGAAACCTGGTTCGAGGTATCCACCGATGGTCATGGAAGGGCACAGATCATGTCAAACCACACTAAAACATTTTGGAGGAACGCAGGTACTACTAATTGGATTTGGTCTGATGTAGAAAGCGCCAACGACCTCACCACCCACAGCAACTTTTGGCCTGTGAAGGTCAACAATAATACCATCGCTCTCAACAGCTCCACCAACAACAATTTTTGCTCACTGATCGCCCTTAGTAGCACAACAGACTGTCTCAACGCTGCCTACTCGAGCATTGCCCCCGAGTCACACCTGGTGATGGAAGAACTCGTGCTCTCCAGAAGCATCTACAATGCCGATTTCGACCTCTCCAATGCCATAATCTATGGCGAGACCCCGGTGACGATGGCCACAGCAAACGCCAGCAACAACACCGATGCAGAAAACACTGTAGAATTCAAGTTCGCCTATGTTGAGAGTAAGAGCAGCACATGGAGTACTAGTCATTCATGGATGGCGGGAGTGAGTGTAACGGCCTCGTTCAAAATTCCATTCATTGGAGGCACCGAGGTCACTGCTACTGCTGAGTATTCCGGATCCTATGAGTGGGGAGAGACTATCACAACCGAAAATACATTGGAGACCACCTACTCGGTTGTCGTGCTGCCAAGAACTTCTATATCCGTGAGCCTCGTAGCCACCAAGGGTTCTTGTGACGTTCCTTATTCCTACTATCAGAGGGATGTGCTCTACAATGGAAAGACCGTCGTGTACAAGAAGGATGACGGTCTGTACACGGGGATCAACTCGTACAATTTCCGTTACGAGGCAACGACCTTAACTCAGGCCAGATCTGATACTCCTGCCACGCTGGTGCAGAAGCTTCCCGACCCATTGACTGACCCGTCCATAGAACAAAAGGTACCGCTGCCATTCCCATCGGCAGAAGCACAGATCTCGCAGCTGTTCTCATCATCAGAGGAGATCGCTCAAGAACCTAACCTGCCAGATGTGGCCCAGCAGTTAGAGGAGACCACAGAGATGCATAAGTTATCTCTGTCACCAAGAGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aerolysin Agglutinin ETX_MTX2 Fascin

Protein Analysis

540

Amino Acids

60.0

Weight (kDa)

5.03

Isoelectric Point (pI)

38.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Agglutinin PF07468 17 - 124 1.7e-16 Agglutinin domain
Aerolysin PF01117 304 - 427 5.6e-08 Aerolysin toxin
ETX_MTX2 PF03318 314 - 426 3.3e-07 Clostridium epsilon toxin ETX/Bacillus mosquitocidal toxin MTX2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000217)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g17710 FvH4_6g45590 FvH4_6g45591 FvH4_6g45770 FvH4_6g45770 FvH4_6g46030 FvH4_6g46040 FvH4_7g07020 FvH4_7g07071 FvH4_7g07080
malus_domestica MD01G1104300.v1.1 MD02G1240000.v1.1 MD02G1240200.v1.1 MD03G1201400.v1.1 MD03G1201500.v1.1 MD07G1171100.v1.1
prunus_persica Prupe.2G087400_v2.0.a1 Prupe.2G097500_v2.0.a1 Prupe.2G097700_v2.0.a1 Prupe.2G097800_v2.0.a1 Prupe.3G153100_v2.0.a1 Prupe.3G153300_v2.0.a1 Prupe.3G153600_v2.0.a1 Prupe.5G237200_v2.0.a1
pyrus_communis pycom03g15270 pycom06g20700 pycom07g06030 pycom10g01810 pycom10g01820 pycom10g12090 pycom14g20090 pycom14g20100 pycom14g20110 pycom14g20120
rosa_chinensis RchiOBHm_Chr1g0336971 RchiOBHm_Chr1g0337031 RchiOBHm_Chr2g0163571 RchiOBHm_Chr2g0163591 RchiOBHm_Chr2g0163631 RchiOBHm_Chr2g0163661 RchiOBHm_Chr2g0163671 RchiOBHm_Chr2g0163691 RchiOBHm_Chr2g0163741 RchiOBHm_Chr5g0011821 RchiOBHm_Chr5g0011831 RchiOBHm_Chr5g0011841 RchiOBHm_Chr5g0053101 RchiOBHm_Chr5g0072501
rosa_laevigata RLG00000010009 RLG00000021413 RLG00000029310 RLG00000029318 RLG00000036313
rosa_multiflora Rmu_co7975848.1_g000001 Rmu_co8020886.1_g000001 Rmu_co8136580.1_g000001 Rmu_co8177372.1_g000001 Rmu_co8207014.1_g000001 Rmu_co8232187.1_g000001 Rmu_co8277595.1_g000001 Rmu_co8389219.1_g000001 Rmu_co8427877.1_g000001 Rmu_co8467587.1_g000001 Rmu_sc0001475.1_g000015 Rmu_sc0001475.1_g000016 Rmu_sc0001475.1_g000017 Rmu_sc0003462.1_g000002 Rmu_sc0003462.1_g000003 Rmu_sc0003484.1_g000002 Rmu_sc0004918.1_g000004 Rmu_sc0004918.1_g000006 Rmu_sc0004918.1_g000007 Rmu_sc0004918.1_g000009 Rmu_sc0006596.1_g000003 Rmu_sc0006596.1_g000005 Rmu_sc0006596.1_g000010 Rmu_sc0008676.1_g000021 Rmu_sc0014330.1_g000002 Rmu_sc0016133.1_g000002 Rmu_sc0016133.1_g000003 Rmu_sc0016133.1_g000004 Rmu_sc0026453.1_g000003 Rmu_sc0026453.1_g000004 Rmu_sc0030660.1_g000001
rosa_roxburghii Rroxscaffold_1G00008700 Rroxscaffold_1G00064740 Rroxscaffold_2G00086950 Rroxscaffold_2G00087100 Rroxscaffold_2G00087140 Rroxscaffold_4G00302080 Rroxscaffold_4G00315020 Rroxscaffold_4G00315170 Rroxscaffold_5G00358800
rosa_rugosa Rorug01G0129200 Rorug01G0130600 Rorug01G0130700 Rorug02G0503700 Rorug02G0503800 Rorug02G0503900 Rorug02G0504000 Rorug02G0504300 Rorug05G0268200 Rorug05G0418700 Rorug05G0418800
rosa_samantha Rh1AG151100 Rh1AG151300 Rh1BG118400 Rh1BG120600 Rh1CG141500 Rh1CG142500 Rh1DG157300 Rh1DG158500 Rh2AG570600 Rh2AG570800 Rh2AG571200 Rh2AG571300 Rh2AG571500 Rh2AG571900 Rh2AG572000 Rh2AG572200 Rh2CG552600 Rh2CG552800 Rh2CG553000 Rh2CG553300 Rh2CG553400 Rh2CG553700 Rh5CG100400 Rh5CG100500 Rh5CG100600 Rh5CG100700 Rh5CG519100 Rh5DG086600 Rh5DG086700 Rh5DG086800 Rh5DG086900 Rh5DG087000 Rh5DG087100 Rh5DG367600 Rh5DG507300
rosa_wichuraiana Rw1G012130 Rw1G012240 Rw1G012380 Rw2G047240 Rw2G047260 Rw2G047270 Rw2G047290 Rw5G007940 Rw5G044120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1453
Acc36I ACCTGC 2 cut(s) 650, 1560
Acc65I GGTACC 1 cut(s) 1471
AccB1I GGYRCC 2 cut(s) 1112, 1471
AccB7I CCANNNNNTGG 1 cut(s) 1106
AccI GTMKAC 1 cut(s) 60
AccIII TCCGGA 1 cut(s) 1142
AciI CCGC 4 cut(s) 137, 162, 1067, 1475
AclWI GGATC 3 cut(s) 1140, 1153, 1358
AcoI YGGCCR 2 cut(s) 159, 957
AcsI RAATTY 3 cut(s) 110, 1004, 1095
AdeI CACNNNGTG 1 cut(s) 865
AfaI GTAC 9 cut(s) 49, 245, 298, 664, 1048, 1322, 1343, 1361, 1473
AfiI CCNNNNNNNGG 5 cut(s) 323, 868, 1106, 1155, 1375
AgsI TTSAA 5 cut(s) 115, 455, 490, 1009, 1093
AhlI ACTAGT 1 cut(s) 1049
AjnI CCWGG 4 cut(s) 231, 435, 581, 861
AleI CACNNNNGTG 1 cut(s) 1421
AluBI AGCT 4 cut(s) 526, 768, 1432, 1513
AluI AGCT 4 cut(s) 526, 768, 1432, 1513
Alw21I GWGCWC 2 cut(s) 885, 1299
Alw26I GTCTC 5 cut(s) 827, 935, 1157, 1184, 1574
AlwI GGATC 3 cut(s) 1140, 1153, 1358
AlwNI CAGNNNCTG 1 cut(s) 260
Ama87I CYCGRG 2 cut(s) 838, 851
Aor13HI TCCGGA 1 cut(s) 1142
AoxI GGCC 9 cut(s) 159, 228, 483, 493, 728, 957, 1083, 1395, 1563
ApaI GGGCCC 1 cut(s) 232
ApeKI GCWGC 6 cut(s) 560, 830, 1035, 1213, 1477, 1510
ApoI RAATTY 3 cut(s) 110, 1004, 1095
Asp700I GAANNNNTTC 1 cut(s) 128
Asp718I GGTACC 1 cut(s) 1471
AspLEI GCGC 1 cut(s) 698
AspS9I GGNCC 3 cut(s) 228, 229, 1564
AsuC2I CCSGG 1 cut(s) 947
AsuHPI GGTGA 4 cut(s) 700, 877, 961, 1602
AsuII TTCGAA 1 cut(s) 150
AsuNHI GCTAGC 1 cut(s) 139
AvaI CYCGRG 2 cut(s) 838, 851
BaeGI GKGCMC 2 cut(s) 232, 619
BalI TGGCCA 1 cut(s) 959
BamHI GGATCC 1 cut(s) 1145
BanI GGYRCC 2 cut(s) 1112, 1471
BanII GRGCYC 1 cut(s) 232
BauI CACGAG 1 cut(s) 878
BbsI GAAGAC 1 cut(s) 282
Bbv12I GWGCWC 2 cut(s) 885, 1299
BbvI GCAGC 6 cut(s) 547, 817, 1047, 1200, 1464, 1522
BccI CCATC 7 cut(s) 317, 596, 761, 862, 949, 1057, 1495
BceAI ACGGC 1 cut(s) 1098
BcgI CGANNNNNNTGC 6 cut(s) 542, 576, 1106, 1140, 1488, 1522
BciT130I CCWGG 4 cut(s) 233, 437, 583, 863
BciVI GTATCC 1 cut(s) 604
BcnI CCSGG 1 cut(s) 947
BcoDI GTCTC 5 cut(s) 827, 935, 1157, 1184, 1574
BcuI ACTAGT 1 cut(s) 1049
BfaI CTAG 3 cut(s) 96, 140, 1050
BfmI CTRYAG 3 cut(s) 318, 362, 999
BfoI RGCGCY 1 cut(s) 699
BfuAI ACCTGC 2 cut(s) 650, 1560
BfuI GTATCC 1 cut(s) 604
BglII AGATCT 2 cut(s) 1400, 1503
BisI GCNGC 7 cut(s) 162, 561, 831, 1036, 1214, 1478, 1511
BlsI GCNGC 7 cut(s) 163, 562, 832, 1037, 1215, 1479, 1512
BmcAI AGTACT 1 cut(s) 1048
Bme1390I CCNGG 5 cut(s) 233, 437, 583, 863, 947
BmeT110I CYCGRG 2 cut(s) 838, 851
BmgT120I GGNCC 3 cut(s) 228, 229, 1564
BmiI GGNNCC 4 cut(s) 230, 1114, 1147, 1473
BmrFI CCNGG 5 cut(s) 233, 437, 583, 863, 947
BmsI GCATC 4 cut(s) 185, 903, 976, 1581
BmtI GCTAGC 1 cut(s) 143
BpiI GAAGAC 1 cut(s) 282
BpmI CTGGAG 2 cut(s) 237, 871
Bpu14I TTCGAA 1 cut(s) 150
BpuEI CTTGAG 1 cut(s) 1524
BpuMI CCSGG 1 cut(s) 947
BsaBI GATNNNNATC 1 cut(s) 327
BsaI GGTCTC 3 cut(s) 935, 1184, 1574
BsaJI CCNNGG 7 cut(s) 231, 232, 473, 564, 945, 1116, 1251
BsaWI WCCGGW 2 cut(s) 373, 1142
BsaXI ACNNNNNCTCC 6 cut(s) 111, 141, 1036, 1062, 1066, 1092
Bsc4I CCNNNNNNNGG 5 cut(s) 323, 868, 1106, 1155, 1375
Bse1I ACTGG 1 cut(s) 175
Bse3DI GCAATG 2 cut(s) 24, 843
Bse8I GATNNNNATC 1 cut(s) 327
BseAI TCCGGA 1 cut(s) 1142
BseBI CCWGG 4 cut(s) 233, 437, 583, 863
BseDI CCNNGG 7 cut(s) 231, 232, 473, 564, 945, 1116, 1251
BseGI GGATG 3 cut(s) 1068, 1297, 1336
BseJI GATNNNNATC 1 cut(s) 327
BseLI CCNNNNNNNGG 5 cut(s) 323, 868, 1106, 1155, 1375
BseMI GCAATG 2 cut(s) 24, 843
BseMII CTCAG 3 cut(s) 104, 1125, 1406
BseNI ACTGG 1 cut(s) 175
BseRI GAGGAG 2 cut(s) 1544, 1592
BseSI GKGCMC 2 cut(s) 232, 619
BseXI GCAGC 6 cut(s) 547, 817, 1047, 1200, 1464, 1522
BseYI CCCAGC 3 cut(s) 42, 423, 1566
BsgI GTGCAG 1 cut(s) 1445
BshFI GGCC 9 cut(s) 161, 230, 485, 495, 730, 959, 1085, 1397, 1565
BshNI GGYRCC 2 cut(s) 1112, 1471
BsiHKAI GWGCWC 2 cut(s) 885, 1299
BsiHKCI CYCGRG 2 cut(s) 838, 851
BsiSI CCGG 3 cut(s) 374, 947, 1143
BslI CCNNNNNNNGG 5 cut(s) 323, 868, 1106, 1155, 1375
BsmAI GTCTC 5 cut(s) 827, 935, 1157, 1184, 1574
BsmI GAATGC 1 cut(s) 548
BsnI GGCC 9 cut(s) 161, 230, 485, 495, 730, 959, 1085, 1397, 1565
Bso31I GGTCTC 3 cut(s) 935, 1184, 1574
BsoBI CYCGRG 2 cut(s) 838, 851
Bsp119I TTCGAA 1 cut(s) 150
Bsp120I GGGCCC 1 cut(s) 228
Bsp1286I GDGCHC 4 cut(s) 232, 619, 885, 1299
Bsp13I TCCGGA 1 cut(s) 1142
Bsp1407I TGTACA 2 cut(s) 1320, 1341
Bsp143I GATC 8 cut(s) 30, 621, 795, 1145, 1350, 1400, 1503, 1533
BspACI CCGC 4 cut(s) 137, 162, 1067, 1475
BspANI GGCC 9 cut(s) 161, 230, 485, 495, 730, 959, 1085, 1397, 1565
BspCNI CTCAG 3 cut(s) 103, 1126, 1405
BspEI TCCGGA 1 cut(s) 1142
BspLI GGNNCC 4 cut(s) 230, 1114, 1147, 1473
BspMI ACCTGC 2 cut(s) 650, 1560
BspOI GCTAGC 1 cut(s) 143
BspPI GGATC 3 cut(s) 1140, 1153, 1358
BspT104I TTCGAA 1 cut(s) 150
BspT107I GGYRCC 2 cut(s) 1112, 1471
BspTNI GGTCTC 3 cut(s) 935, 1184, 1574
BsrDI GCAATG 2 cut(s) 24, 843
BsrGI TGTACA 2 cut(s) 1320, 1341
BsrI ACTGG 1 cut(s) 175
BssECI CCNNGG 7 cut(s) 231, 232, 473, 564, 945, 1116, 1251
BssMI GATC 8 cut(s) 30, 621, 795, 1145, 1350, 1400, 1503, 1533
BssSI CACGAG 1 cut(s) 878
BssT1I CCWWGG 2 cut(s) 564, 1251
Bst2BI CACGAG 1 cut(s) 878
Bst2UI CCWGG 4 cut(s) 233, 437, 583, 863
Bst4CI ACNGT 6 cut(s) 520, 540, 821, 1000, 1315, 1337
BstAUI TGTACA 2 cut(s) 1320, 1341
BstBI TTCGAA 1 cut(s) 150
BstC8I GCNNGC 3 cut(s) 137, 141, 973
BstDEI CTNAG 4 cut(s) 90, 803, 1134, 1392
BstF5I GGATG 3 cut(s) 1068, 1297, 1336
BstH2I RGCGCY 1 cut(s) 699
BstHHI GCGC 1 cut(s) 698
BstKTI GATC 8 cut(s) 33, 624, 798, 1148, 1353, 1403, 1506, 1536
BstMAI GTCTC 5 cut(s) 827, 935, 1157, 1184, 1574
BstMBI GATC 8 cut(s) 30, 621, 795, 1145, 1350, 1400, 1503, 1533
BstNI CCWGG 4 cut(s) 233, 437, 583, 863
BstNSI RCATGY 1 cut(s) 482
BstSCI CCNGG 5 cut(s) 231, 435, 581, 861, 945
BstSFI CTRYAG 3 cut(s) 318, 362, 999
BstSLI GKGCMC 2 cut(s) 232, 619
BstV1I GCAGC 6 cut(s) 547, 817, 1047, 1200, 1464, 1522
BstV2I GAAGAC 1 cut(s) 282
BstX2I RGATCY 3 cut(s) 1145, 1400, 1503
BstXI CCANNNNNNTGG 1 cut(s) 239
BstYI RGATCY 3 cut(s) 1145, 1400, 1503
BsuI GTATCC 1 cut(s) 604
BsuRI GGCC 9 cut(s) 161, 230, 485, 495, 730, 959, 1085, 1397, 1565
BtgZI GCGATG 1 cut(s) 739
BtsCI GGATG 3 cut(s) 1068, 1297, 1336
BtsI GCAGTG 2 cut(s) 244, 1122
BtsIMutI CAGTG 4 cut(s) 244, 791, 996, 1122
BveI ACCTGC 2 cut(s) 650, 1560
Cac8I GCNNGC 3 cut(s) 137, 141, 973
CaiI CAGNNNCTG 1 cut(s) 260
CfoI GCGC 1 cut(s) 698
Cfr13I GGNCC 3 cut(s) 228, 229, 1564
CseI GACGC 1 cut(s) 266
CsiI ACCWGGT 2 cut(s) 581, 861
Csp6I GTAC 9 cut(s) 48, 244, 297, 663, 1047, 1321, 1342, 1360, 1472
CviAII CATG 8 cut(s) 64, 294, 353, 479, 608, 625, 1041, 1059
CviQI GTAC 9 cut(s) 48, 244, 297, 663, 1047, 1321, 1342, 1360, 1472
DdeI CTNAG 4 cut(s) 90, 803, 1134, 1392
DpnI GATC 8 cut(s) 32, 623, 797, 1147, 1352, 1402, 1505, 1535
DpnII GATC 8 cut(s) 30, 621, 795, 1145, 1350, 1400, 1503, 1533
DraIII CACNNNGTG 1 cut(s) 865
DrdI GACNNNNNNGTC 1 cut(s) 1453
DseDI GACNNNNNNGTC 1 cut(s) 1453
EaeI YGGCCR 2 cut(s) 159, 957
Eco130I CCWWGG 2 cut(s) 564, 1251
Eco24I GRGCYC 1 cut(s) 232
Eco31I GGTCTC 3 cut(s) 935, 1184, 1574
Eco32I GATATC 1 cut(s) 433
Eco88I CYCGRG 2 cut(s) 838, 851
EcoO109I RGGNCCY 1 cut(s) 228
EcoRI GAATTC 1 cut(s) 1004
EcoRII CCWGG 4 cut(s) 231, 435, 581, 861
EcoRV GATATC 1 cut(s) 433
EcoT14I CCWWGG 2 cut(s) 564, 1251
EcoT22I ATGCAT 1 cut(s) 1596
EcoT38I GRGCYC 1 cut(s) 232
ErhI CCWWGG 2 cut(s) 564, 1251
FaeI CATG 8 cut(s) 67, 297, 356, 482, 611, 628, 1044, 1062
FalI AAGNNNNNCTT 2 cut(s) 200, 232
FatI CATG 8 cut(s) 63, 293, 352, 478, 607, 624, 1040, 1058
FauI CCCGC 2 cut(s) 144, 1060
FblI GTMKAC 1 cut(s) 60
Fnu4HI GCNGC 7 cut(s) 162, 561, 831, 1036, 1214, 1478, 1511
FokI GGATG 3 cut(s) 1075, 1304, 1343
FriOI GRGCYC 1 cut(s) 232
Fsp4HI GCNGC 7 cut(s) 162, 561, 831, 1036, 1214, 1478, 1511
FspBI CTAG 3 cut(s) 96, 140, 1050
GlaI GCGC 1 cut(s) 697
GluI GCNGC 7 cut(s) 162, 561, 831, 1036, 1214, 1478, 1511
GsaI CCCAGC 3 cut(s) 46, 427, 1570
GsuI CTGGAG 2 cut(s) 237, 871
HaeII RGCGCY 1 cut(s) 699
HaeIII GGCC 9 cut(s) 161, 230, 485, 495, 730, 959, 1085, 1397, 1565
HapII CCGG 3 cut(s) 374, 947, 1143
HgaI GACGC 1 cut(s) 266
HhaI GCGC 1 cut(s) 698
Hin1II CATG 8 cut(s) 67, 297, 356, 482, 611, 628, 1044, 1062
Hin6I GCGC 1 cut(s) 696
HinP1I GCGC 1 cut(s) 696
HincII GTYRAC 1 cut(s) 742
HindII GTYRAC 1 cut(s) 742
HindIII AAGCTT 1 cut(s) 1430
HinfI GANTC 4 cut(s) 80, 86, 455, 854
HpaII CCGG 3 cut(s) 374, 947, 1143
HphI GGTGA 4 cut(s) 700, 877, 961, 1602
Hpy166II GTNNAC 4 cut(s) 61, 742, 1321, 1344
Hpy188I TCNGA 7 cut(s) 85, 93, 515, 685, 1287, 1405, 1528
Hpy188III TCNNGA 6 cut(s) 254, 332, 888, 1143, 1436, 1541
Hpy8I GTNNAC 4 cut(s) 61, 742, 1321, 1344
Hpy99I CGWCG 1 cut(s) 1319
HpyAV CCTTC 4 cut(s) 496, 606, 730, 1321
HpyCH4III ACNGT 6 cut(s) 520, 540, 821, 1000, 1315, 1337
HpyCH4IV ACGT 3 cut(s) 57, 299, 1266
HpyCH4V TGCA 4 cut(s) 187, 989, 1426, 1594
HpyF3I CTNAG 4 cut(s) 90, 803, 1134, 1392
HpySE526I ACGT 3 cut(s) 57, 299, 1266
Hsp92II CATG 8 cut(s) 67, 297, 356, 482, 611, 628, 1044, 1062
HspAI GCGC 1 cut(s) 696
Kpn2I TCCGGA 1 cut(s) 1142
KpnI GGTACC 1 cut(s) 1475
Kzo9I GATC 8 cut(s) 30, 621, 795, 1145, 1350, 1400, 1503, 1533
LmnI GCTCC 1 cut(s) 773
Lsp1109I GCAGC 6 cut(s) 547, 817, 1047, 1200, 1464, 1522
LweI GCATC 4 cut(s) 185, 903, 976, 1581
MabI ACCWGGT 2 cut(s) 581, 861
MaeI CTAG 3 cut(s) 96, 140, 1050
MaeII ACGT 3 cut(s) 57, 299, 1266
MaeIII GTNAC 8 cut(s) 383, 855, 949, 1078, 1120, 1262, 1370, 1608
MalI GATC 8 cut(s) 32, 623, 797, 1147, 1352, 1402, 1505, 1535
MboI GATC 8 cut(s) 30, 621, 795, 1145, 1350, 1400, 1503, 1533
MboII GAAGA 3 cut(s) 284, 287, 884
MflI RGATCY 3 cut(s) 1145, 1400, 1503
MhlI GDGCHC 4 cut(s) 232, 619, 885, 1299
MlsI TGGCCA 1 cut(s) 959
MluCI AATT 6 cut(s) 110, 670, 781, 1004, 1095, 1363
MluNI TGGCCA 1 cut(s) 959
MlyI GAGTC 1 cut(s) 863
MmeI TCCRAC 1 cut(s) 382
Mox20I TGGCCA 1 cut(s) 959
Mph1103I ATGCAT 1 cut(s) 1596
MroI TCCGGA 1 cut(s) 1142
MroXI GAANNNNTTC 1 cut(s) 128
MscI TGGCCA 1 cut(s) 959
MseI TTAA 1 cut(s) 1388
MslI CAYNNNNRTG 2 cut(s) 1421, 1589
Msp20I TGGCCA 1 cut(s) 959
MspA1I CMGCKG 2 cut(s) 1477, 1513
MspI CCGG 3 cut(s) 374, 947, 1143
MspR9I CCNGG 5 cut(s) 233, 437, 583, 863, 947
Mva1269I GAATGC 1 cut(s) 548
MvaI CCWGG 4 cut(s) 233, 437, 583, 863
NciI CCSGG 1 cut(s) 947
NdeII GATC 8 cut(s) 30, 621, 795, 1145, 1350, 1400, 1503, 1533
NheI GCTAGC 1 cut(s) 139
NlaIII CATG 8 cut(s) 67, 297, 356, 482, 611, 628, 1044, 1062
NlaIV GGNNCC 4 cut(s) 230, 1114, 1147, 1473
NmeAIII GCCGAG 1 cut(s) 498
NmuCI GTSAC 5 cut(s) 855, 949, 1120, 1262, 1608
NsiI ATGCAT 1 cut(s) 1596
NspI RCATGY 1 cut(s) 482
NspV TTCGAA 1 cut(s) 150
OliI CACNNNNGTG 1 cut(s) 1421
PaeR7I CTCGAG 1 cut(s) 838
PasI CCCWGGG 1 cut(s) 232
PctI GAATGC 1 cut(s) 548
PdmI GAANNNNTTC 1 cut(s) 128
PfeI GAWTC 3 cut(s) 80, 86, 455
PflMI CCANNNNNTGG 1 cut(s) 1106
PfoI TCCNGGA 1 cut(s) 435
PkrI GCNGC 7 cut(s) 163, 562, 832, 1037, 1215, 1479, 1512
PleI GAGTC 1 cut(s) 862
PpsI GAGTC 1 cut(s) 862
Psp6I CCWGG 4 cut(s) 231, 435, 581, 861
PspFI CCCAGC 3 cut(s) 42, 423, 1566
PspGI CCWGG 4 cut(s) 231, 435, 581, 861
PspN4I GGNNCC 4 cut(s) 230, 1114, 1147, 1473
PspOMI GGGCCC 1 cut(s) 228
PspPI GGNCC 3 cut(s) 228, 229, 1564
PspXI VCTCGAGB 1 cut(s) 838
PsrI GAACNNNNNNTAC 2 cut(s) 1455, 1487
PstNI CAGNNNCTG 1 cut(s) 260
PsuI RGATCY 3 cut(s) 1145, 1400, 1503
PvuII CAGCTG 1 cut(s) 1513
RsaI GTAC 9 cut(s) 49, 245, 298, 664, 1048, 1322, 1343, 1361, 1473
RsaNI GTAC 9 cut(s) 48, 244, 297, 663, 1047, 1321, 1342, 1360, 1472
RseI CAYNNNNRTG 2 cut(s) 1421, 1589
SaqAI TTAA 1 cut(s) 1388
SatI GCNGC 7 cut(s) 162, 561, 831, 1036, 1214, 1478, 1511
Sau3AI GATC 8 cut(s) 30, 621, 795, 1145, 1350, 1400, 1503, 1533
Sau96I GGNCC 3 cut(s) 228, 229, 1564
ScaI AGTACT 1 cut(s) 1048
SchI GAGTC 1 cut(s) 863
ScrFI CCNGG 5 cut(s) 233, 437, 583, 863, 947
SduI GDGCHC 4 cut(s) 232, 619, 885, 1299
SexAI ACCWGGT 2 cut(s) 581, 861
SfaNI GCATC 4 cut(s) 185, 903, 976, 1581
SfcI CTRYAG 3 cut(s) 318, 362, 999
Sfr274I CTCGAG 1 cut(s) 838
SfuI TTCGAA 1 cut(s) 150
SlaI CTCGAG 1 cut(s) 838
SmiMI CAYNNNNRTG 2 cut(s) 1421, 1589
SmlI CTYRAG 2 cut(s) 838, 1539
SmoI CTYRAG 2 cut(s) 838, 1539
SpeI ACTAGT 1 cut(s) 1049
Sse9I AATT 6 cut(s) 110, 670, 781, 1004, 1095, 1363
SsiI CCGC 4 cut(s) 137, 162, 1067, 1475
SspMI CTAG 3 cut(s) 96, 140, 1050
StyD4I CCNGG 5 cut(s) 231, 435, 581, 861, 945
StyI CCWWGG 2 cut(s) 564, 1251
TaaI ACNGT 6 cut(s) 520, 540, 821, 1000, 1315, 1337
TaiI ACGT 3 cut(s) 60, 302, 1269
TaqI TCGA 5 cut(s) 150, 552, 588, 839, 912
TasI AATT 6 cut(s) 110, 670, 781, 1004, 1095, 1363
TatI WGTACW 3 cut(s) 1046, 1320, 1341
TauI GCSGC 1 cut(s) 164
TfiI GAWTC 3 cut(s) 80, 86, 455
Tru1I TTAA 1 cut(s) 1388
Tru9I TTAA 1 cut(s) 1388
TscAI CASTG 4 cut(s) 244, 798, 1003, 1129
TseFI GTSAC 5 cut(s) 855, 949, 1120, 1262, 1608
TseI GCWGC 6 cut(s) 560, 830, 1035, 1213, 1477, 1510
Tsp45I GTSAC 5 cut(s) 855, 949, 1120, 1262, 1608
TspDTI ATGAA 4 cut(s) 80, 279, 1047, 1093
TspGWI ACGGA 2 cut(s) 1222, 1358
TspRI CASTG 4 cut(s) 244, 798, 1003, 1129
Van91I CCANNNNNTGG 1 cut(s) 1106
XapI RAATTY 3 cut(s) 110, 1004, 1095
XceI RCATGY 1 cut(s) 482
XhoI CTCGAG 1 cut(s) 838
XmiI GTMKAC 1 cut(s) 60
XmnI GAANNNNTTC 1 cut(s) 128
XspI CTAG 3 cut(s) 96, 140, 1050
ZrmI AGTACT 1 cut(s) 1048
Zsp2I ATGCAT 1 cut(s) 1596
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.