Rw5G044120

Agglutinin domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000217
Physical Location & Seq
Forward (+)
77510357 .. 77512384
2028 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G044120.1

Sequence Viewer

Length: 1620 bp
ATGTCGGTTCTATTACCTCCCACCGTTGCCATTAAATCAGTCGGGTCCGTCCAGGTATACATGAACTACAACGACGATTCTAATTCTCAGACTAATGGCTTCCTCAAATTCAATGGAGGGAATGTTTTCAGCCCGCTAGCAAAGTTCGAAGTGGTGACCGCAAATACTGGAATAGGAATGGTGCATATAAGATGCTCTCACAATAAGAAGTTCTTGAGAGTGAGGGCCCAGGGCAGTGCGTACATTTCTCCGGACGCTGATGAACCAGAAGAAGACCAATCAAAATGGTCATGTACGTTATTCCAGCCCAGACCTGTAGATGGCAATCCAGACCGCGTTCGGTTGTTCCATGTCCAACTCCAGAAGTATCCGGTGGTCGCTGTTTCTTATTACTTTTGGGTGGGCTTTGATAATCCAGGCCTGCAGGATATCTTGGAAATCATTGACCTTGAATCGTTAGTGATACTGCCGAGGCATGTGGCCTTCAAAGGCCATAATGGAAAATATCTCGGAACAGTAGAGCTGAACAACCAACGGTATCTGGCATTCGATTACGCTGCTAAGGGTCGCCCTGAAACCTGGTTCGAGGTGTCCACCGATGGTCATGGAAGGGCACAGATCATGTCAAACCACACAAAGACATTTTGGAGGAACGCGGGTGCTACTAATTGGATTTGGTCTGATGTAGAAAGCGCCAACGACCTCACAACCCACAGCAACTTTTGGCCTGTGAAGGTCAACAATAATACCATCGCTCTCAACAGCTCCACCAACAACAATTTTTGCTCACTGATCGCCCTTAATAGCACAACAGACTGTCTCAACGCTGCCTACTCTAGCATTGCCCCCGAGTCACACCTGGTGATGGAAGAACTCGTGCTCTCCAGAAGCATCTACAATGCCGATTTCGACCTCGCCAATGCCATAATCTATGGCGAGACCCCCGTGACGATGGCCACAGCAAACGCCAGCAACAACACCGATGCAGAAAACACTGTAGAATTCAAGTTCGCCTATGTTGAGAGTAAGAGCAGCACATGGAGTACTAGTCATTCATGGATGGCGGGAGTGAGTGTAACGGCCTCGTTCAAAATTCCATTCATTGGAGGCACCGAGGTCACTGCTACTGCTGAGTATTCCGGATCCTATGAGTGGGGAGAAACTATCACAACCGAAAATACATTGGAGACCACCTACTCGGTTGTCGTGCTGCCAAGAACTTCTATATCCGTGAGCCTCGTAGCCACCAAGGGTTCTTGTGACGTTCCTTATTCCTACTATCAGAGGGATGTGCTCTACAATGGAAAGACCGTCGTGTACAAGAAGGATGACGGTCTGTACACGGGGATCAACTCGTACAATTTCCGTTACGAGGCAACGACTTTAACTCAGGCCAGATCTGATGCTCCTGCCACGCTGGTGCAGAAGCTTCCCGACCCATTGACTGACCCGTCCATAGAACAAAAGGTACCGCTGCCATTCCCATCGGCAGAAGCACAGATCTCGCAGCTGTTCTCATCATCAGAGGAGATCGCTCAAGAACCTAACCCGCCAGATGTGGCCCAGCAGTTAGAGGAGACCACAGAGATGCATAAGTTATCTCTGTCACCAAGAGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aerolysin Agglutinin ETX_MTX2 Fascin

Protein Analysis

539

Amino Acids

59.74

Weight (kDa)

5.03

Isoelectric Point (pI)

37.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Agglutinin PF07468 8 - 123 1.1e-17 Agglutinin domain
Aerolysin PF01117 303 - 426 3.3e-08 Aerolysin toxin
ETX_MTX2 PF03318 313 - 425 1.6e-08 Clostridium epsilon toxin ETX/Bacillus mosquitocidal toxin MTX2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000217)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g17710 FvH4_6g45590 FvH4_6g45591 FvH4_6g45770 FvH4_6g45770 FvH4_6g46030 FvH4_6g46040 FvH4_7g07020 FvH4_7g07071 FvH4_7g07080
malus_domestica MD01G1104300.v1.1 MD02G1240000.v1.1 MD02G1240200.v1.1 MD03G1201400.v1.1 MD03G1201500.v1.1 MD07G1171100.v1.1
prunus_persica Prupe.2G087400_v2.0.a1 Prupe.2G097500_v2.0.a1 Prupe.2G097700_v2.0.a1 Prupe.2G097800_v2.0.a1 Prupe.3G153100_v2.0.a1 Prupe.3G153300_v2.0.a1 Prupe.3G153600_v2.0.a1 Prupe.5G237200_v2.0.a1
pyrus_communis pycom03g15270 pycom06g20700 pycom07g06030 pycom10g01810 pycom10g01820 pycom10g12090 pycom14g20090 pycom14g20100 pycom14g20110 pycom14g20120
rosa_chinensis RchiOBHm_Chr1g0336971 RchiOBHm_Chr1g0337031 RchiOBHm_Chr2g0163571 RchiOBHm_Chr2g0163591 RchiOBHm_Chr2g0163631 RchiOBHm_Chr2g0163661 RchiOBHm_Chr2g0163671 RchiOBHm_Chr2g0163691 RchiOBHm_Chr2g0163741 RchiOBHm_Chr5g0011821 RchiOBHm_Chr5g0011831 RchiOBHm_Chr5g0011841 RchiOBHm_Chr5g0053101 RchiOBHm_Chr5g0072501
rosa_laevigata RLG00000010009 RLG00000021413 RLG00000029310 RLG00000029318 RLG00000036313
rosa_multiflora Rmu_co7975848.1_g000001 Rmu_co8020886.1_g000001 Rmu_co8136580.1_g000001 Rmu_co8177372.1_g000001 Rmu_co8207014.1_g000001 Rmu_co8232187.1_g000001 Rmu_co8277595.1_g000001 Rmu_co8389219.1_g000001 Rmu_co8427877.1_g000001 Rmu_co8467587.1_g000001 Rmu_sc0001475.1_g000015 Rmu_sc0001475.1_g000016 Rmu_sc0001475.1_g000017 Rmu_sc0003462.1_g000002 Rmu_sc0003462.1_g000003 Rmu_sc0003484.1_g000002 Rmu_sc0004918.1_g000004 Rmu_sc0004918.1_g000006 Rmu_sc0004918.1_g000007 Rmu_sc0004918.1_g000009 Rmu_sc0006596.1_g000003 Rmu_sc0006596.1_g000005 Rmu_sc0006596.1_g000010 Rmu_sc0008676.1_g000021 Rmu_sc0014330.1_g000002 Rmu_sc0016133.1_g000002 Rmu_sc0016133.1_g000003 Rmu_sc0016133.1_g000004 Rmu_sc0026453.1_g000003 Rmu_sc0026453.1_g000004 Rmu_sc0030660.1_g000001
rosa_roxburghii Rroxscaffold_1G00008700 Rroxscaffold_1G00064740 Rroxscaffold_2G00086950 Rroxscaffold_2G00087100 Rroxscaffold_2G00087140 Rroxscaffold_4G00302080 Rroxscaffold_4G00315020 Rroxscaffold_4G00315170 Rroxscaffold_5G00358800
rosa_rugosa Rorug01G0129200 Rorug01G0130600 Rorug01G0130700 Rorug02G0503700 Rorug02G0503800 Rorug02G0503900 Rorug02G0504000 Rorug02G0504300 Rorug05G0268200 Rorug05G0418700 Rorug05G0418800
rosa_samantha Rh1AG151100 Rh1AG151300 Rh1BG118400 Rh1BG120600 Rh1CG141500 Rh1CG142500 Rh1DG157300 Rh1DG158500 Rh2AG570600 Rh2AG570800 Rh2AG571200 Rh2AG571300 Rh2AG571500 Rh2AG571900 Rh2AG572000 Rh2AG572200 Rh2CG552600 Rh2CG552800 Rh2CG553000 Rh2CG553300 Rh2CG553400 Rh2CG553700 Rh5CG100400 Rh5CG100500 Rh5CG100600 Rh5CG100700 Rh5CG519100 Rh5DG086600 Rh5DG086700 Rh5DG086800 Rh5DG086900 Rh5DG087000 Rh5DG087100 Rh5DG367600 Rh5DG507300
rosa_wichuraiana Rw1G012130 Rw1G012240 Rw1G012380 Rw2G047240 Rw2G047260 Rw2G047270 Rw2G047290 Rw5G007940 Rw5G044120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1450
Acc65I GGTACC 1 cut(s) 1468
AccB1I GGYRCC 2 cut(s) 1109, 1468
AccB7I CCANNNNNTGG 1 cut(s) 1103
AccI GTMKAC 1 cut(s) 57
AccII CGCG 2 cut(s) 336, 656
AccIII TCCGGA 2 cut(s) 250, 1139
AciI CCGC 7 cut(s) 134, 159, 334, 656, 1064, 1472, 1550
AclWI GGATC 3 cut(s) 1137, 1150, 1355
AcoI YGGCCR 1 cut(s) 954
AcsI RAATTY 3 cut(s) 107, 1001, 1092
AdeI CACNNNGTG 1 cut(s) 862
AfaI GTAC 7 cut(s) 242, 295, 1045, 1319, 1340, 1358, 1470
AfiI CCNNNNNNNGG 5 cut(s) 320, 865, 1103, 1152, 1372
AgsI TTSAA 5 cut(s) 112, 452, 487, 1006, 1090
AhlI ACTAGT 1 cut(s) 1046
AjnI CCWGG 5 cut(s) 51, 228, 415, 578, 858
AleI CACNNNNGTG 1 cut(s) 1418
AluBI AGCT 4 cut(s) 523, 765, 1429, 1510
AluI AGCT 4 cut(s) 523, 765, 1429, 1510
Alw21I GWGCWC 2 cut(s) 882, 1296
Alw26I GTCTC 4 cut(s) 824, 932, 1181, 1571
AlwI GGATC 3 cut(s) 1137, 1150, 1355
Ama87I CYCGRG 1 cut(s) 848
Aor13HI TCCGGA 2 cut(s) 250, 1139
AoxI GGCC 9 cut(s) 225, 418, 480, 490, 725, 954, 1080, 1392, 1560
ApaI GGGCCC 1 cut(s) 229
ApeKI GCWGC 6 cut(s) 557, 827, 1032, 1210, 1474, 1507
ApoI RAATTY 3 cut(s) 107, 1001, 1092
Asp700I GAANNNNTTC 1 cut(s) 125
Asp718I GGTACC 1 cut(s) 1468
AspLEI GCGC 1 cut(s) 695
AspS9I GGNCC 4 cut(s) 45, 225, 226, 1561
AsuHPI GGTGA 3 cut(s) 166, 874, 1599
AsuII TTCGAA 1 cut(s) 147
AsuNHI GCTAGC 1 cut(s) 136
AvaI CYCGRG 1 cut(s) 848
AvaII GGWCC 1 cut(s) 45
BaeGI GKGCMC 2 cut(s) 229, 616
BalI TGGCCA 1 cut(s) 956
BamHI GGATCC 1 cut(s) 1142
BanI GGYRCC 2 cut(s) 1109, 1468
BanII GRGCYC 1 cut(s) 229
BauI CACGAG 1 cut(s) 875
BbsI GAAGAC 1 cut(s) 279
Bbv12I GWGCWC 2 cut(s) 882, 1296
BbvI GCAGC 6 cut(s) 544, 814, 1044, 1197, 1461, 1519
BccI CCATC 7 cut(s) 314, 593, 758, 859, 946, 1054, 1492
BceAI ACGGC 1 cut(s) 1095
BcgI CGANNNNNNTGC 6 cut(s) 539, 573, 1103, 1137, 1485, 1519
BciT130I CCWGG 5 cut(s) 53, 230, 417, 580, 860
BciVI GTATCC 1 cut(s) 378
BcoDI GTCTC 4 cut(s) 824, 932, 1181, 1571
BcuI ACTAGT 1 cut(s) 1046
BfaI CTAG 3 cut(s) 137, 837, 1047
BfmI CTRYAG 3 cut(s) 315, 422, 996
BfoI RGCGCY 1 cut(s) 696
BfuI GTATCC 1 cut(s) 378
BglII AGATCT 2 cut(s) 1397, 1500
BisI GCNGC 6 cut(s) 558, 828, 1033, 1211, 1475, 1508
BlsI GCNGC 6 cut(s) 559, 829, 1034, 1212, 1476, 1509
BmcAI AGTACT 1 cut(s) 1045
Bme1390I CCNGG 5 cut(s) 53, 230, 417, 580, 860
Bme18I GGWCC 1 cut(s) 45
BmeT110I CYCGRG 1 cut(s) 848
BmgT120I GGNCC 4 cut(s) 45, 225, 226, 1561
BmiI GGNNCC 5 cut(s) 46, 227, 1111, 1144, 1470
BmrFI CCNGG 5 cut(s) 53, 230, 417, 580, 860
BmsI GCATC 5 cut(s) 182, 900, 973, 1393, 1578
BmtI GCTAGC 1 cut(s) 140
BpiI GAAGAC 1 cut(s) 279
BpmI CTGGAG 2 cut(s) 344, 868
Bpu10I CCTNAGC 1 cut(s) 561
Bpu14I TTCGAA 1 cut(s) 147
BpuEI CTTGAG 2 cut(s) 235, 1521
BsaBI GATNNNNATC 1 cut(s) 324
BsaI GGTCTC 3 cut(s) 932, 1181, 1571
BsaJI CCNNGG 5 cut(s) 228, 229, 470, 1113, 1248
BsaWI WCCGGW 3 cut(s) 250, 370, 1139
BsaXI ACNNNNNCTCC 6 cut(s) 108, 138, 1033, 1059, 1063, 1089
Bsc4I CCNNNNNNNGG 5 cut(s) 320, 865, 1103, 1152, 1372
Bse1I ACTGG 1 cut(s) 172
Bse3DI GCAATG 1 cut(s) 840
Bse8I GATNNNNATC 1 cut(s) 324
BseAI TCCGGA 2 cut(s) 250, 1139
BseBI CCWGG 5 cut(s) 53, 230, 417, 580, 860
BseDI CCNNGG 5 cut(s) 228, 229, 470, 1113, 1248
BseGI GGATG 3 cut(s) 1065, 1294, 1333
BseJI GATNNNNATC 1 cut(s) 324
BseLI CCNNNNNNNGG 5 cut(s) 320, 865, 1103, 1152, 1372
BseMI GCAATG 1 cut(s) 840
BseMII CTCAG 3 cut(s) 101, 1122, 1403
BseNI ACTGG 1 cut(s) 172
BseRI GAGGAG 2 cut(s) 1541, 1589
BseSI GKGCMC 2 cut(s) 229, 616
BseXI GCAGC 6 cut(s) 544, 814, 1044, 1197, 1461, 1519
BseYI CCCAGC 1 cut(s) 1563
BsgI GTGCAG 1 cut(s) 1442
Bsh1236I CGCG 2 cut(s) 336, 656
BshFI GGCC 9 cut(s) 227, 420, 482, 492, 727, 956, 1082, 1394, 1562
BshNI GGYRCC 2 cut(s) 1109, 1468
BsiHKAI GWGCWC 2 cut(s) 882, 1296
BsiHKCI CYCGRG 1 cut(s) 848
BsiSI CCGG 3 cut(s) 251, 371, 1140
BslI CCNNNNNNNGG 5 cut(s) 320, 865, 1103, 1152, 1372
BsmAI GTCTC 4 cut(s) 824, 932, 1181, 1571
BsmI GAATGC 1 cut(s) 545
BsnI GGCC 9 cut(s) 227, 420, 482, 492, 727, 956, 1082, 1394, 1562
Bso31I GGTCTC 3 cut(s) 932, 1181, 1571
BsoBI CYCGRG 1 cut(s) 848
Bsp119I TTCGAA 1 cut(s) 147
Bsp120I GGGCCC 1 cut(s) 225
Bsp1286I GDGCHC 4 cut(s) 229, 616, 882, 1296
Bsp13I TCCGGA 2 cut(s) 250, 1139
Bsp1407I TGTACA 2 cut(s) 1317, 1338
Bsp143I GATC 7 cut(s) 618, 792, 1142, 1347, 1397, 1500, 1530
BspACI CCGC 7 cut(s) 134, 159, 334, 656, 1064, 1472, 1550
BspANI GGCC 9 cut(s) 227, 420, 482, 492, 727, 956, 1082, 1394, 1562
BspCNI CTCAG 3 cut(s) 100, 1123, 1402
BspEI TCCGGA 2 cut(s) 250, 1139
BspFNI CGCG 2 cut(s) 336, 656
BspLI GGNNCC 5 cut(s) 46, 227, 1111, 1144, 1470
BspMAI CTGCAG 1 cut(s) 426
BspOI GCTAGC 1 cut(s) 140
BspPI GGATC 3 cut(s) 1137, 1150, 1355
BspT104I TTCGAA 1 cut(s) 147
BspT107I GGYRCC 2 cut(s) 1109, 1468
BspTNI GGTCTC 3 cut(s) 932, 1181, 1571
BsrDI GCAATG 1 cut(s) 840
BsrGI TGTACA 2 cut(s) 1317, 1338
BsrI ACTGG 1 cut(s) 172
BssECI CCNNGG 5 cut(s) 228, 229, 470, 1113, 1248
BssMI GATC 7 cut(s) 618, 792, 1142, 1347, 1397, 1500, 1530
BssNAI GTATAC 1 cut(s) 58
BssSI CACGAG 1 cut(s) 875
BssT1I CCWWGG 1 cut(s) 1248
Bst1107I GTATAC 1 cut(s) 58
Bst2BI CACGAG 1 cut(s) 875
Bst2UI CCWGG 5 cut(s) 53, 230, 417, 580, 860
Bst4CI ACNGT 7 cut(s) 25, 517, 537, 818, 997, 1312, 1334
BstAUI TGTACA 2 cut(s) 1317, 1338
BstBI TTCGAA 1 cut(s) 147
BstC8I GCNNGC 4 cut(s) 134, 138, 422, 970
BstDEI CTNAG 4 cut(s) 87, 561, 1131, 1389
BstEII GGTNACC 1 cut(s) 154
BstF5I GGATG 3 cut(s) 1065, 1294, 1333
BstFNI CGCG 2 cut(s) 336, 656
BstH2I RGCGCY 1 cut(s) 696
BstHHI GCGC 1 cut(s) 695
BstKTI GATC 7 cut(s) 621, 795, 1145, 1350, 1400, 1503, 1533
BstMAI GTCTC 4 cut(s) 824, 932, 1181, 1571
BstMBI GATC 7 cut(s) 618, 792, 1142, 1347, 1397, 1500, 1530
BstNI CCWGG 5 cut(s) 53, 230, 417, 580, 860
BstNSI RCATGY 1 cut(s) 479
BstPI GGTNACC 1 cut(s) 154
BstSCI CCNGG 5 cut(s) 51, 228, 415, 578, 858
BstSFI CTRYAG 3 cut(s) 315, 422, 996
BstSLI GKGCMC 2 cut(s) 229, 616
BstUI CGCG 2 cut(s) 336, 656
BstV1I GCAGC 6 cut(s) 544, 814, 1044, 1197, 1461, 1519
BstV2I GAAGAC 1 cut(s) 279
BstX2I RGATCY 3 cut(s) 1142, 1397, 1500
BstYI RGATCY 3 cut(s) 1142, 1397, 1500
BstZ17I GTATAC 1 cut(s) 58
BsuI GTATCC 1 cut(s) 378
BsuRI GGCC 9 cut(s) 227, 420, 482, 492, 727, 956, 1082, 1394, 1562
BtgZI GCGATG 1 cut(s) 736
BtsCI GGATG 3 cut(s) 1065, 1294, 1333
BtsI GCAGTG 2 cut(s) 241, 1119
BtsIMutI CAGTG 4 cut(s) 241, 788, 993, 1119
Cac8I GCNNGC 4 cut(s) 134, 138, 422, 970
CfoI GCGC 1 cut(s) 695
Cfr13I GGNCC 4 cut(s) 45, 225, 226, 1561
CseI GACGC 1 cut(s) 263
CsiI ACCWGGT 2 cut(s) 578, 858
Csp6I GTAC 7 cut(s) 241, 294, 1044, 1318, 1339, 1357, 1469
CviAII CATG 8 cut(s) 61, 291, 350, 476, 605, 622, 1038, 1056
CviQI GTAC 7 cut(s) 241, 294, 1044, 1318, 1339, 1357, 1469
DdeI CTNAG 4 cut(s) 87, 561, 1131, 1389
DpnI GATC 7 cut(s) 620, 794, 1144, 1349, 1399, 1502, 1532
DpnII GATC 7 cut(s) 618, 792, 1142, 1347, 1397, 1500, 1530
DraIII CACNNNGTG 1 cut(s) 862
DrdI GACNNNNNNGTC 1 cut(s) 1450
DseDI GACNNNNNNGTC 1 cut(s) 1450
EaeI YGGCCR 1 cut(s) 954
Eco130I CCWWGG 1 cut(s) 1248
Eco147I AGGCCT 1 cut(s) 420
Eco24I GRGCYC 1 cut(s) 229
Eco31I GGTCTC 3 cut(s) 932, 1181, 1571
Eco32I GATATC 1 cut(s) 430
Eco47I GGWCC 1 cut(s) 45
Eco88I CYCGRG 1 cut(s) 848
Eco91I GGTNACC 1 cut(s) 154
EcoO109I RGGNCCY 1 cut(s) 225
EcoO65I GGTNACC 1 cut(s) 154
EcoRI GAATTC 1 cut(s) 1001
EcoRII CCWGG 5 cut(s) 51, 228, 415, 578, 858
EcoRV GATATC 1 cut(s) 430
EcoT14I CCWWGG 1 cut(s) 1248
EcoT22I ATGCAT 1 cut(s) 1593
EcoT38I GRGCYC 1 cut(s) 229
ErhI CCWWGG 1 cut(s) 1248
FaeI CATG 8 cut(s) 64, 294, 353, 479, 608, 625, 1041, 1059
FalI AAGNNNNNCTT 2 cut(s) 197, 229
FatI CATG 8 cut(s) 60, 290, 349, 475, 604, 621, 1037, 1055
FauI CCCGC 4 cut(s) 141, 649, 1057, 1557
FblI GTMKAC 1 cut(s) 57
Fnu4HI GCNGC 6 cut(s) 558, 828, 1033, 1211, 1475, 1508
FokI GGATG 3 cut(s) 1072, 1301, 1340
FriOI GRGCYC 1 cut(s) 229
Fsp4HI GCNGC 6 cut(s) 558, 828, 1033, 1211, 1475, 1508
FspBI CTAG 3 cut(s) 137, 837, 1047
GlaI GCGC 1 cut(s) 694
GluI GCNGC 6 cut(s) 558, 828, 1033, 1211, 1475, 1508
GsaI CCCAGC 1 cut(s) 1567
GsuI CTGGAG 2 cut(s) 344, 868
HaeII RGCGCY 1 cut(s) 696
HaeIII GGCC 9 cut(s) 227, 420, 482, 492, 727, 956, 1082, 1394, 1562
HapII CCGG 3 cut(s) 251, 371, 1140
HgaI GACGC 1 cut(s) 263
HhaI GCGC 1 cut(s) 695
Hin1II CATG 8 cut(s) 64, 294, 353, 479, 608, 625, 1041, 1059
Hin6I GCGC 1 cut(s) 693
HinP1I GCGC 1 cut(s) 693
HincII GTYRAC 1 cut(s) 739
HindII GTYRAC 1 cut(s) 739
HindIII AAGCTT 1 cut(s) 1427
HinfI GANTC 3 cut(s) 77, 452, 851
HpaII CCGG 3 cut(s) 251, 371, 1140
HphI GGTGA 3 cut(s) 166, 874, 1599
Hpy166II GTNNAC 5 cut(s) 58, 594, 739, 1318, 1341
Hpy188I TCNGA 6 cut(s) 90, 512, 682, 1284, 1402, 1525
Hpy188III TCNNGA 8 cut(s) 214, 251, 329, 361, 885, 1140, 1433, 1538
Hpy8I GTNNAC 5 cut(s) 58, 594, 739, 1318, 1341
Hpy99I CGWCG 2 cut(s) 77, 1316
HpyAV CCTTC 4 cut(s) 493, 603, 727, 1318
HpyCH4III ACNGT 7 cut(s) 25, 517, 537, 818, 997, 1312, 1334
HpyCH4IV ACGT 2 cut(s) 296, 1263
HpyCH4V TGCA 5 cut(s) 184, 424, 986, 1423, 1591
HpyF3I CTNAG 4 cut(s) 87, 561, 1131, 1389
HpySE526I ACGT 2 cut(s) 296, 1263
Hsp92II CATG 8 cut(s) 64, 294, 353, 479, 608, 625, 1041, 1059
HspAI GCGC 1 cut(s) 693
Kpn2I TCCGGA 2 cut(s) 250, 1139
KpnI GGTACC 1 cut(s) 1472
Kzo9I GATC 7 cut(s) 618, 792, 1142, 1347, 1397, 1500, 1530
LmnI GCTCC 2 cut(s) 770, 1411
Lsp1109I GCAGC 6 cut(s) 544, 814, 1044, 1197, 1461, 1519
LweI GCATC 5 cut(s) 182, 900, 973, 1393, 1578
MabI ACCWGGT 2 cut(s) 578, 858
MaeI CTAG 3 cut(s) 137, 837, 1047
MaeII ACGT 2 cut(s) 296, 1263
MaeIII GTNAC 8 cut(s) 154, 852, 946, 1075, 1117, 1259, 1367, 1605
MalI GATC 7 cut(s) 620, 794, 1144, 1349, 1399, 1502, 1532
MboI GATC 7 cut(s) 618, 792, 1142, 1347, 1397, 1500, 1530
MboII GAAGA 3 cut(s) 281, 284, 881
MflI RGATCY 3 cut(s) 1142, 1397, 1500
MhlI GDGCHC 4 cut(s) 229, 616, 882, 1296
MlsI TGGCCA 1 cut(s) 956
MluCI AATT 7 cut(s) 82, 107, 667, 778, 1001, 1092, 1360
MluNI TGGCCA 1 cut(s) 956
MlyI GAGTC 1 cut(s) 860
MmeI TCCRAC 1 cut(s) 379
Mox20I TGGCCA 1 cut(s) 956
Mph1103I ATGCAT 1 cut(s) 1593
MroI TCCGGA 2 cut(s) 250, 1139
MroXI GAANNNNTTC 1 cut(s) 125
MscI TGGCCA 1 cut(s) 956
MseI TTAA 3 cut(s) 33, 801, 1385
MslI CAYNNNNRTG 2 cut(s) 1418, 1586
Msp20I TGGCCA 1 cut(s) 956
MspA1I CMGCKG 2 cut(s) 1474, 1510
MspI CCGG 3 cut(s) 251, 371, 1140
MspR9I CCNGG 5 cut(s) 53, 230, 417, 580, 860
Mva1269I GAATGC 1 cut(s) 545
MvaI CCWGG 5 cut(s) 53, 230, 417, 580, 860
MvnI CGCG 2 cut(s) 336, 656
NdeII GATC 7 cut(s) 618, 792, 1142, 1347, 1397, 1500, 1530
NheI GCTAGC 1 cut(s) 136
NlaIII CATG 8 cut(s) 64, 294, 353, 479, 608, 625, 1041, 1059
NlaIV GGNNCC 5 cut(s) 46, 227, 1111, 1144, 1470
NmeAIII GCCGAG 1 cut(s) 495
NmuCI GTSAC 6 cut(s) 154, 852, 946, 1117, 1259, 1605
NsiI ATGCAT 1 cut(s) 1593
NspI RCATGY 1 cut(s) 479
NspV TTCGAA 1 cut(s) 147
OliI CACNNNNGTG 1 cut(s) 1418
PasI CCCWGGG 1 cut(s) 229
PceI AGGCCT 1 cut(s) 420
PctI GAATGC 1 cut(s) 545
PdmI GAANNNNTTC 1 cut(s) 125
PfeI GAWTC 2 cut(s) 77, 452
PflMI CCANNNNNTGG 1 cut(s) 1103
PkrI GCNGC 6 cut(s) 559, 829, 1034, 1212, 1476, 1509
PleI GAGTC 1 cut(s) 859
PpsI GAGTC 1 cut(s) 859
Psp6I CCWGG 5 cut(s) 51, 228, 415, 578, 858
PspEI GGTNACC 1 cut(s) 154
PspFI CCCAGC 1 cut(s) 1563
PspGI CCWGG 5 cut(s) 51, 228, 415, 578, 858
PspN4I GGNNCC 5 cut(s) 46, 227, 1111, 1144, 1470
PspOMI GGGCCC 1 cut(s) 225
PspPI GGNCC 4 cut(s) 45, 225, 226, 1561
PsrI GAACNNNNNNTAC 2 cut(s) 1452, 1484
PstI CTGCAG 1 cut(s) 426
PsuI RGATCY 3 cut(s) 1142, 1397, 1500
PvuII CAGCTG 1 cut(s) 1510
RsaI GTAC 7 cut(s) 242, 295, 1045, 1319, 1340, 1358, 1470
RsaNI GTAC 7 cut(s) 241, 294, 1044, 1318, 1339, 1357, 1469
RseI CAYNNNNRTG 2 cut(s) 1418, 1586
SaqAI TTAA 3 cut(s) 33, 801, 1385
SatI GCNGC 6 cut(s) 558, 828, 1033, 1211, 1475, 1508
Sau3AI GATC 7 cut(s) 618, 792, 1142, 1347, 1397, 1500, 1530
Sau96I GGNCC 4 cut(s) 45, 225, 226, 1561
SbfI CCTGCAGG 1 cut(s) 426
ScaI AGTACT 1 cut(s) 1045
SchI GAGTC 1 cut(s) 860
ScrFI CCNGG 5 cut(s) 53, 230, 417, 580, 860
SdaI CCTGCAGG 1 cut(s) 426
SduI GDGCHC 4 cut(s) 229, 616, 882, 1296
SexAI ACCWGGT 2 cut(s) 578, 858
SfaNI GCATC 5 cut(s) 182, 900, 973, 1393, 1578
SfcI CTRYAG 3 cut(s) 315, 422, 996
SfuI TTCGAA 1 cut(s) 147
SinI GGWCC 1 cut(s) 45
SmiMI CAYNNNNRTG 2 cut(s) 1418, 1586
SmlI CTYRAG 2 cut(s) 214, 1536
SmoI CTYRAG 2 cut(s) 214, 1536
SpeI ACTAGT 1 cut(s) 1046
Sse8387I CCTGCAGG 1 cut(s) 426
Sse9I AATT 7 cut(s) 82, 107, 667, 778, 1001, 1092, 1360
SseBI AGGCCT 1 cut(s) 420
SsiI CCGC 7 cut(s) 134, 159, 334, 656, 1064, 1472, 1550
SspMI CTAG 3 cut(s) 137, 837, 1047
StuI AGGCCT 1 cut(s) 420
StyD4I CCNGG 5 cut(s) 51, 228, 415, 578, 858
StyI CCWWGG 1 cut(s) 1248
TaaI ACNGT 7 cut(s) 25, 517, 537, 818, 997, 1312, 1334
TaiI ACGT 2 cut(s) 299, 1266
TaqI TCGA 4 cut(s) 147, 549, 585, 909
TasI AATT 7 cut(s) 82, 107, 667, 778, 1001, 1092, 1360
TatI WGTACW 3 cut(s) 1043, 1317, 1338
TfiI GAWTC 2 cut(s) 77, 452
Tru1I TTAA 3 cut(s) 33, 801, 1385
Tru9I TTAA 3 cut(s) 33, 801, 1385
TscAI CASTG 4 cut(s) 241, 795, 1000, 1126
TseFI GTSAC 6 cut(s) 154, 852, 946, 1117, 1259, 1605
TseI GCWGC 6 cut(s) 557, 827, 1032, 1210, 1474, 1507
Tsp45I GTSAC 6 cut(s) 154, 852, 946, 1117, 1259, 1605
TspDTI ATGAA 4 cut(s) 77, 276, 1044, 1090
TspGWI ACGGA 3 cut(s) 37, 1219, 1355
TspRI CASTG 4 cut(s) 241, 795, 1000, 1126
Van91I CCANNNNNTGG 1 cut(s) 1103
VpaK11BI GGWCC 1 cut(s) 45
XapI RAATTY 3 cut(s) 107, 1001, 1092
XceI RCATGY 1 cut(s) 479
XmiI GTMKAC 1 cut(s) 57
XmnI GAANNNNTTC 1 cut(s) 125
XspI CTAG 3 cut(s) 137, 837, 1047
ZrmI AGTACT 1 cut(s) 1045
Zsp2I ATGCAT 1 cut(s) 1593
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.