Rh5CG100600

Agglutinin domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0000217
Physical Location & Seq
Forward (+)
8045497 .. 8047056
1560 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG100600.1

Sequence Viewer

Length: 1560 bp
ATGTCGTTCCTATTACCACCCACTATTACTCTGAAATCATCCTATTTCAACCAGTACGTGAACTACAGCAATGATTCTGATTCTGAAACGCATGGCTTTCTCAAAGTCAACGGATCAAATGTCTTCAGCTCGTTAGCAAAGTTCGAAGTGGTGACGGCCAATACTGGGATGGGACTAGTCCATATAAGGTGCTCCCAGAATAGAAAGTTCCTGCGAACGAGAATTGAGGGTGATGTGTACCTTTCTCCGGAGGCTGATGAACCAGAGGAGGACCAATCCAAATGGTCGTGCACACTATTTGAGCCTCAAGCAGCTGTAGATGGCAACCCCGACAAAGCCCGGTTGTTCCATGTCCAAGCAAAGCGATATGCAACGGTATTGGACGATGGTGGAGTAAGGTTCTTCAAATTAGATGACGGTCAGAGTGATCAGGGTATCTTCGATATCACTGACTGGCAGTCATTGGTGATATTGCCAAAGCATGTGGCCTTCAAAGCCAACAACAACCTTTACCTTCAGCTTGGGGAAAGTAATCAGCTCAGGTTCGTGTCCACCGATAAGGGGATTCCTGCTTCGTGGTTCGAAGTGTCCACAGATGGCGATGGAATGGTGAAAATCAAATGCTACAAGAACAATTCCTACCTGAGGTTCGGCGGCAGTGACTACAGCAACTGGATTTTTGCTGACGGAGGCAGCGCATCGAAACCTCCCACCTTGTTTTTGCCAATCAAGGTCGACACTAGTGCGGCCGCTCTCAAATGCTTGGGCAACGACAAATTTTGCGCCCTACACACCTATGAGTATGATTATACAGACGGTCTGAACGCGCCTGACACTAGTACAAGCATTTCAAACTTGTCACGCTGGGGGCTGGAAGAGCTTGTTCTCTCTAGGAGAATCTATAATGCCGACTTTGACCTTAAGAATGCCATAATCTACGGCGAGACCCCGGTCACCATGGCCACCGCAAACGCGAGCAACAATACCTCAGCAGAAAACACTGTGGAATTCAAGTTTGCATACAAGGATGGTAAGAGCAGCACGTGGAAGGCCAGCCACTCGTGGATGATAGGCATGAGTGTCTCTGCCAGCTTCAAAATTCCATTCATCGGCGGTACTGACGTCACTGCTCAAGCAGAGTATTCCGGATCCTATGAGTGGGGAGAGACCATTACATCTGAAAACACATTGGAGACCACCTACAAGGCTACAGTGCCGGCAAACACTTCTATAACTGTGAGCCTGATGGCGACCAAGGGAAGTTGTGACGTGCCTTACTCGTATTATCAGAAGGACGTGCTTTATAACGGTAAATCCGTTATCTATAAGAAGGATGATGGGGTGTACACGGGTGTCAATTCATACAATTTCCGCTACGAGACTACAGCACGGAAAGAGCCGGTATCTATAGCTGATTTGCTGGACCCGTCGGCCGACCCAACCACAGAGCAGATGTTGCCGGACCCATCGGTCGACCCCACCATAGAACAAATGATACCTGAGCCTGTGCCGTCTGGATCAACCACCAGAGTGCAAAACAAGTTCAAAACCATAGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aerolysin Agglutinin ETX_MTX2 Fascin

Protein Analysis

519

Amino Acids

57.48

Weight (kDa)

5.0

Isoelectric Point (pI)

30.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Agglutinin PF07468 5 - 136 6.5e-15 Agglutinin domain
Aerolysin PF01117 308 - 428 9.7e-09 Aerolysin toxin
ETX_MTX2 PF03318 315 - 429 2.2e-08 Clostridium epsilon toxin ETX/Bacillus mosquitocidal toxin MTX2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000217)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g17710 FvH4_6g45590 FvH4_6g45591 FvH4_6g45770 FvH4_6g45770 FvH4_6g46030 FvH4_6g46040 FvH4_7g07020 FvH4_7g07071 FvH4_7g07080
malus_domestica MD01G1104300.v1.1 MD02G1240000.v1.1 MD02G1240200.v1.1 MD03G1201400.v1.1 MD03G1201500.v1.1 MD07G1171100.v1.1
prunus_persica Prupe.2G087400_v2.0.a1 Prupe.2G097500_v2.0.a1 Prupe.2G097700_v2.0.a1 Prupe.2G097800_v2.0.a1 Prupe.3G153100_v2.0.a1 Prupe.3G153300_v2.0.a1 Prupe.3G153600_v2.0.a1 Prupe.5G237200_v2.0.a1
pyrus_communis pycom03g15270 pycom06g20700 pycom07g06030 pycom10g01810 pycom10g01820 pycom10g12090 pycom14g20090 pycom14g20100 pycom14g20110 pycom14g20120
rosa_chinensis RchiOBHm_Chr1g0336971 RchiOBHm_Chr1g0337031 RchiOBHm_Chr2g0163571 RchiOBHm_Chr2g0163591 RchiOBHm_Chr2g0163631 RchiOBHm_Chr2g0163661 RchiOBHm_Chr2g0163671 RchiOBHm_Chr2g0163691 RchiOBHm_Chr2g0163741 RchiOBHm_Chr5g0011821 RchiOBHm_Chr5g0011831 RchiOBHm_Chr5g0011841 RchiOBHm_Chr5g0053101 RchiOBHm_Chr5g0072501
rosa_laevigata RLG00000010009 RLG00000021413 RLG00000029310 RLG00000029318 RLG00000036313
rosa_multiflora Rmu_co7975848.1_g000001 Rmu_co8020886.1_g000001 Rmu_co8136580.1_g000001 Rmu_co8177372.1_g000001 Rmu_co8207014.1_g000001 Rmu_co8232187.1_g000001 Rmu_co8277595.1_g000001 Rmu_co8389219.1_g000001 Rmu_co8427877.1_g000001 Rmu_co8467587.1_g000001 Rmu_sc0001475.1_g000015 Rmu_sc0001475.1_g000016 Rmu_sc0001475.1_g000017 Rmu_sc0003462.1_g000002 Rmu_sc0003462.1_g000003 Rmu_sc0003484.1_g000002 Rmu_sc0004918.1_g000004 Rmu_sc0004918.1_g000006 Rmu_sc0004918.1_g000007 Rmu_sc0004918.1_g000009 Rmu_sc0006596.1_g000003 Rmu_sc0006596.1_g000005 Rmu_sc0006596.1_g000010 Rmu_sc0008676.1_g000021 Rmu_sc0014330.1_g000002 Rmu_sc0016133.1_g000002 Rmu_sc0016133.1_g000003 Rmu_sc0016133.1_g000004 Rmu_sc0026453.1_g000003 Rmu_sc0026453.1_g000004 Rmu_sc0030660.1_g000001
rosa_roxburghii Rroxscaffold_1G00008700 Rroxscaffold_1G00064740 Rroxscaffold_2G00086950 Rroxscaffold_2G00087100 Rroxscaffold_2G00087140 Rroxscaffold_4G00302080 Rroxscaffold_4G00315020 Rroxscaffold_4G00315170 Rroxscaffold_5G00358800
rosa_rugosa Rorug01G0129200 Rorug01G0130600 Rorug01G0130700 Rorug02G0503700 Rorug02G0503800 Rorug02G0503900 Rorug02G0504000 Rorug02G0504300 Rorug05G0268200 Rorug05G0418700 Rorug05G0418800
rosa_samantha Rh1AG151100 Rh1AG151300 Rh1BG118400 Rh1BG120600 Rh1CG141500 Rh1CG142500 Rh1DG157300 Rh1DG158500 Rh2AG570600 Rh2AG570800 Rh2AG571200 Rh2AG571300 Rh2AG571500 Rh2AG571900 Rh2AG572000 Rh2AG572200 Rh2CG552600 Rh2CG552800 Rh2CG553000 Rh2CG553300 Rh2CG553400 Rh2CG553700 Rh5CG100400 Rh5CG100500 Rh5CG100600 Rh5CG100700 Rh5CG519100 Rh5DG086600 Rh5DG086700 Rh5DG086800 Rh5DG086900 Rh5DG087000 Rh5DG087100 Rh5DG367600 Rh5DG507300
rosa_wichuraiana Rw1G012130 Rw1G012240 Rw1G012380 Rw2G047240 Rw2G047260 Rw2G047270 Rw2G047290 Rw5G007940 Rw5G044120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1305
AasI GACNNNNNNGTC 1 cut(s) 1469
AatII GACGTC 1 cut(s) 1125
AccBSI CCGCTC 1 cut(s) 752
AccI GTMKAC 2 cut(s) 735, 1473
AccII CGCG 2 cut(s) 827, 974
AccIII TCCGGA 2 cut(s) 247, 1145
AciI CCGC 6 cut(s) 654, 746, 750, 966, 1113, 1372
AclWI GGATC 4 cut(s) 121, 1143, 1156, 1525
AcoI YGGCCR 4 cut(s) 156, 747, 960, 1431
AcsI RAATTY 3 cut(s) 776, 1007, 1098
AcuI CTGAAG 2 cut(s) 109, 500
AcvI CACGTG 1 cut(s) 1044
AcyI GRCGYC 1 cut(s) 1122
AfaI GTAC 5 cut(s) 56, 239, 841, 1117, 1346
AfiI CCNNNNNNNGG 6 cut(s) 165, 247, 561, 645, 1109, 1158
AflII CTTAAG 1 cut(s) 920
AgsI TTSAA 7 cut(s) 49, 406, 493, 852, 1012, 1096, 1546
AhdI GACNNNNNGTC 1 cut(s) 457
AhlI ACTAGT 3 cut(s) 175, 740, 836
AjiI CACGTC 2 cut(s) 1270, 1297
AleI CACNNNNGTG 1 cut(s) 1529
AluBI AGCT 8 cut(s) 129, 314, 520, 538, 880, 1092, 1412, 1556
AluI AGCT 8 cut(s) 129, 314, 520, 538, 880, 1092, 1412, 1556
Alw21I GWGCWC 2 cut(s) 194, 293
Alw26I GTCTC 5 cut(s) 938, 1087, 1160, 1187, 1373
Alw44I GTGCAC 1 cut(s) 289
AlwI GGATC 4 cut(s) 121, 1143, 1156, 1525
AlwNI CAGNNNCTG 1 cut(s) 672
Aor13HI TCCGGA 2 cut(s) 247, 1145
AoxI GGCC 6 cut(s) 156, 486, 747, 960, 1050, 1431
ApaLI GTGCAC 1 cut(s) 289
ApeKI GCWGC 3 cut(s) 311, 693, 1038
ApoI RAATTY 3 cut(s) 776, 1007, 1098
AspLEI GCGC 3 cut(s) 698, 785, 829
AspS9I GGNCC 3 cut(s) 271, 1423, 1462
AsuC2I CCSGG 2 cut(s) 340, 950
AsuHPI GGTGA 5 cut(s) 163, 242, 478, 622, 946
AsuII TTCGAA 2 cut(s) 144, 582
AvaII GGWCC 3 cut(s) 271, 1423, 1462
AxyI CCTNAGG 1 cut(s) 644
BaeGI GKGCMC 1 cut(s) 293
BaeI ACNNNNGTAYC 2 cut(s) 1107, 1140
BalI TGGCCA 1 cut(s) 962
BamHI GGATCC 1 cut(s) 1148
BauI CACGAG 1 cut(s) 1060
BbrPI CACGTG 1 cut(s) 1044
BbsI GAAGAC 1 cut(s) 115
Bbv12I GWGCWC 2 cut(s) 194, 293
BbvCI CCTCAGC 1 cut(s) 988
BbvI GCAGC 3 cut(s) 323, 705, 1050
BccI CCATC 9 cut(s) 163, 314, 380, 590, 596, 1022, 1240, 1331, 1474
BceAI ACGGC 3 cut(s) 171, 955, 1495
BcgI CGANNNNNNTGC 2 cut(s) 725, 759
BclI TGATCA 1 cut(s) 427
BcnI CCSGG 2 cut(s) 340, 950
BcoDI GTCTC 5 cut(s) 938, 1087, 1160, 1187, 1373
BcuI ACTAGT 3 cut(s) 175, 740, 836
BfaI CTAG 4 cut(s) 176, 741, 837, 891
BfmI CTRYAG 6 cut(s) 64, 315, 664, 1209, 1383, 1407
BfrI CTTAAG 1 cut(s) 920
BisI GCNGC 6 cut(s) 312, 655, 694, 747, 750, 1039
BlsI GCNGC 6 cut(s) 313, 656, 695, 748, 751, 1040
Bme1390I CCNGG 2 cut(s) 340, 950
Bme18I GGWCC 3 cut(s) 271, 1423, 1462
BmeRI GACNNNNNGTC 1 cut(s) 457
BmgBI CACGTC 2 cut(s) 1270, 1297
BmgT120I GGNCC 3 cut(s) 271, 1423, 1462
BmiI GGNNCC 3 cut(s) 1150, 1425, 1464
BmrFI CCNGG 2 cut(s) 340, 950
BmrI ACTGGG 1 cut(s) 174
BmsI GCATC 1 cut(s) 707
BmuI ACTGGG 1 cut(s) 174
BoxI GACNNNNGTC 1 cut(s) 950
BpiI GAAGAC 1 cut(s) 115
Bpu10I CCTNAGC 3 cut(s) 539, 988, 1500
Bpu14I TTCGAA 2 cut(s) 144, 582
BpuEI CTTGAG 2 cut(s) 291, 1116
BpuMI CCSGG 2 cut(s) 340, 950
BsaAI YACGTR 2 cut(s) 58, 1044
BsaHI GRCGYC 1 cut(s) 1122
BsaI GGTCTC 3 cut(s) 938, 1160, 1187
BsaJI CCNNGG 3 cut(s) 948, 957, 1254
BsaWI WCCGGW 2 cut(s) 247, 1145
Bsc4I CCNNNNNNNGG 6 cut(s) 165, 247, 561, 645, 1109, 1158
Bse118I RCCGGY 2 cut(s) 1216, 1399
Bse1I ACTGG 4 cut(s) 52, 169, 458, 677
Bse21I CCTNAGG 1 cut(s) 644
Bse3DI GCAATG 1 cut(s) 76
BseAI TCCGGA 2 cut(s) 247, 1145
BseDI CCNNGG 3 cut(s) 948, 957, 1254
BseGI GGATG 5 cut(s) 38, 174, 1033, 1071, 1339
BseLI CCNNNNNNNGG 6 cut(s) 165, 247, 561, 645, 1109, 1158
BseMI GCAATG 1 cut(s) 76
BseMII CTCAG 4 cut(s) 553, 635, 1002, 1491
BseNI ACTGG 4 cut(s) 52, 169, 458, 677
BseRI GAGGAG 1 cut(s) 281
BseSI GKGCMC 1 cut(s) 293
BseX3I CGGCCG 2 cut(s) 747, 1431
BseXI GCAGC 3 cut(s) 323, 705, 1050
BseYI CCCAGC 1 cut(s) 864
Bsh1236I CGCG 2 cut(s) 827, 974
Bsh1285I CGRYCG 3 cut(s) 750, 1434, 1473
BshFI GGCC 6 cut(s) 158, 488, 749, 962, 1052, 1433
BsiEI CGRYCG 3 cut(s) 750, 1434, 1473
BsiHKAI GWGCWC 2 cut(s) 194, 293
BsiSI CCGG 7 cut(s) 248, 340, 950, 1146, 1217, 1400, 1460
BslFI GGGAC 1 cut(s) 186
BslI CCNNNNNNNGG 6 cut(s) 165, 247, 561, 645, 1109, 1158
BsmAI GTCTC 5 cut(s) 938, 1087, 1160, 1187, 1373
BsmFI GGGAC 1 cut(s) 186
BsmI GAATGC 1 cut(s) 931
BsnI GGCC 6 cut(s) 158, 488, 749, 962, 1052, 1433
Bso31I GGTCTC 3 cut(s) 938, 1160, 1187
Bsp119I TTCGAA 2 cut(s) 144, 582
Bsp1286I GDGCHC 2 cut(s) 194, 293
Bsp13I TCCGGA 2 cut(s) 247, 1145
Bsp1407I TGTACA 1 cut(s) 1344
Bsp143I GATC 4 cut(s) 113, 427, 1148, 1517
Bsp19I CCATGG 1 cut(s) 957
BspACI CCGC 6 cut(s) 654, 746, 750, 966, 1113, 1372
BspANI GGCC 6 cut(s) 158, 488, 749, 962, 1052, 1433
BspCNI CTCAG 4 cut(s) 552, 636, 1001, 1492
BspEI TCCGGA 2 cut(s) 247, 1145
BspFNI CGCG 2 cut(s) 827, 974
BspLI GGNNCC 3 cut(s) 1150, 1425, 1464
BspPI GGATC 4 cut(s) 121, 1143, 1156, 1525
BspQI GCTCTTC 1 cut(s) 870
BspT104I TTCGAA 2 cut(s) 144, 582
BspTI CTTAAG 1 cut(s) 920
BspTNI GGTCTC 3 cut(s) 938, 1160, 1187
BsrBI CCGCTC 1 cut(s) 752
BsrDI GCAATG 1 cut(s) 76
BsrFI RCCGGY 2 cut(s) 1216, 1399
BsrGI TGTACA 1 cut(s) 1344
BsrI ACTGG 4 cut(s) 52, 169, 458, 677
BssAI RCCGGY 2 cut(s) 1216, 1399
BssECI CCNNGG 3 cut(s) 948, 957, 1254
BssMI GATC 4 cut(s) 113, 427, 1148, 1517
BssNI GRCGYC 1 cut(s) 1122
BssSI CACGAG 1 cut(s) 1060
BssT1I CCWWGG 2 cut(s) 957, 1254
Bst2BI CACGAG 1 cut(s) 1060
Bst4CI ACNGT 7 cut(s) 376, 419, 818, 1003, 1213, 1237, 1310
Bst6I CTCTTC 1 cut(s) 870
BstACI GRCGYC 1 cut(s) 1122
BstAFI CTTAAG 1 cut(s) 920
BstAPI GCANNNNNTGC 1 cut(s) 1456
BstAUI TGTACA 1 cut(s) 1344
BstBAI YACGTR 2 cut(s) 58, 1044
BstBI TTCGAA 2 cut(s) 144, 582
BstC8I GCNNGC 4 cut(s) 976, 1054, 1090, 1218
BstDEI CTNAG 4 cut(s) 539, 644, 988, 1500
BstDSI CCRYGG 1 cut(s) 957
BstEII GGTNACC 1 cut(s) 952
BstENI CCTNNNNNAGG 1 cut(s) 643
BstF5I GGATG 5 cut(s) 38, 174, 1033, 1071, 1339
BstFNI CGCG 2 cut(s) 827, 974
BstHHI GCGC 3 cut(s) 698, 785, 829
BstKTI GATC 4 cut(s) 116, 430, 1151, 1520
BstMAI GTCTC 5 cut(s) 938, 1087, 1160, 1187, 1373
BstMBI GATC 4 cut(s) 113, 427, 1148, 1517
BstMCI CGRYCG 3 cut(s) 750, 1434, 1473
BstMWI GCNNNNNNNGC 3 cut(s) 494, 877, 1456
BstNSI RCATGY 1 cut(s) 485
BstPAI GACNNNNGTC 1 cut(s) 950
BstPI GGTNACC 1 cut(s) 952
BstSCI CCNGG 2 cut(s) 338, 948
BstSFI CTRYAG 6 cut(s) 64, 315, 664, 1209, 1383, 1407
BstSLI GKGCMC 1 cut(s) 293
BstUI CGCG 2 cut(s) 827, 974
BstV1I GCAGC 3 cut(s) 323, 705, 1050
BstV2I GAAGAC 1 cut(s) 115
BstX2I RGATCY 1 cut(s) 1148
BstYI RGATCY 1 cut(s) 1148
BstZI CGGCCG 2 cut(s) 747, 1431
Bsu36I CCTNAGG 1 cut(s) 644
BsuRI GGCC 6 cut(s) 158, 488, 749, 962, 1052, 1433
BtgI CCRYGG 1 cut(s) 957
BtgZI GCGATG 1 cut(s) 615
BtrI CACGTC 2 cut(s) 1270, 1297
BtsCI GGATG 5 cut(s) 38, 174, 1033, 1071, 1339
BtsI GCAGTG 2 cut(s) 664, 1125
BtsIMutI CAGTG 5 cut(s) 447, 664, 999, 1125, 1218
Cac8I GCNNGC 4 cut(s) 976, 1054, 1090, 1218
CaiI CAGNNNCTG 1 cut(s) 672
CciNI GCGGCCGC 1 cut(s) 747
CfoI GCGC 3 cut(s) 698, 785, 829
Cfr10I RCCGGY 2 cut(s) 1216, 1399
Cfr13I GGNCC 3 cut(s) 271, 1423, 1462
Csp6I GTAC 5 cut(s) 55, 238, 840, 1116, 1345
CspCI CAANNNNNGTGG 2 cut(s) 465, 500
CviAII CATG 5 cut(s) 92, 350, 482, 958, 1075
CviQI GTAC 5 cut(s) 55, 238, 840, 1116, 1345
DdeI CTNAG 4 cut(s) 539, 644, 988, 1500
DpnI GATC 4 cut(s) 115, 429, 1150, 1519
DpnII GATC 4 cut(s) 113, 427, 1148, 1517
DrdI GACNNNNNNGTC 1 cut(s) 1469
DriI GACNNNNNGTC 1 cut(s) 457
DseDI GACNNNNNNGTC 1 cut(s) 1469
EaeI YGGCCR 4 cut(s) 156, 747, 960, 1431
EagI CGGCCG 2 cut(s) 747, 1431
Eam1104I CTCTTC 1 cut(s) 870
Eam1105I GACNNNNNGTC 1 cut(s) 457
EarI CTCTTC 1 cut(s) 870
EclXI CGGCCG 2 cut(s) 747, 1431
Eco130I CCWWGG 2 cut(s) 957, 1254
Eco31I GGTCTC 3 cut(s) 938, 1160, 1187
Eco32I GATATC 1 cut(s) 445
Eco47I GGWCC 3 cut(s) 271, 1423, 1462
Eco52I CGGCCG 2 cut(s) 747, 1431
Eco57I CTGAAG 2 cut(s) 109, 500
Eco72I CACGTG 1 cut(s) 1044
Eco81I CCTNAGG 1 cut(s) 644
Eco91I GGTNACC 1 cut(s) 952
EcoNI CCTNNNNNAGG 1 cut(s) 643
EcoO65I GGTNACC 1 cut(s) 952
EcoRI GAATTC 1 cut(s) 1007
EcoRV GATATC 1 cut(s) 445
EcoT14I CCWWGG 2 cut(s) 957, 1254
ErhI CCWWGG 2 cut(s) 957, 1254
FaeI CATG 5 cut(s) 95, 353, 485, 961, 1078
FaqI GGGAC 1 cut(s) 186
FatI CATG 5 cut(s) 91, 349, 481, 957, 1074
FbaI TGATCA 1 cut(s) 427
FblI GTMKAC 2 cut(s) 735, 1473
Fnu4HI GCNGC 6 cut(s) 312, 655, 694, 747, 750, 1039
FokI GGATG 5 cut(s) 25, 181, 1040, 1078, 1346
Fsp4HI GCNGC 6 cut(s) 312, 655, 694, 747, 750, 1039
FspBI CTAG 4 cut(s) 176, 741, 837, 891
GlaI GCGC 3 cut(s) 697, 784, 828
GluI GCNGC 6 cut(s) 312, 655, 694, 747, 750, 1039
GsaI CCCAGC 1 cut(s) 868
HaeIII GGCC 6 cut(s) 158, 488, 749, 962, 1052, 1433
HapII CCGG 7 cut(s) 248, 340, 950, 1146, 1217, 1400, 1460
HhaI GCGC 3 cut(s) 698, 785, 829
Hin1I GRCGYC 1 cut(s) 1122
Hin1II CATG 5 cut(s) 95, 353, 485, 961, 1078
Hin6I GCGC 3 cut(s) 696, 783, 827
HinP1I GCGC 3 cut(s) 696, 783, 827
HincII GTYRAC 3 cut(s) 109, 736, 1474
HindII GTYRAC 3 cut(s) 109, 736, 1474
HinfI GANTC 4 cut(s) 74, 80, 565, 897
HpaII CCGG 7 cut(s) 248, 340, 950, 1146, 1217, 1400, 1460
HphI GGTGA 5 cut(s) 163, 242, 478, 622, 946
Hpy188I TCNGA 7 cut(s) 33, 79, 85, 423, 822, 1180, 1290
Hpy188III TCNNGA 3 cut(s) 248, 1146, 1515
Hpy99I CGWCG 1 cut(s) 1432
HpyAV CCTTC 5 cut(s) 499, 524, 1042, 1285, 1324
HpyCH4III ACNGT 7 cut(s) 376, 419, 818, 1003, 1213, 1237, 1310
HpyCH4IV ACGT 5 cut(s) 57, 1043, 1122, 1269, 1296
HpyCH4V TGCA 4 cut(s) 291, 371, 1019, 1534
HpyF10VI GCNNNNNNNGC 3 cut(s) 494, 877, 1456
HpyF3I CTNAG 4 cut(s) 539, 644, 988, 1500
HpySE526I ACGT 5 cut(s) 57, 1043, 1122, 1269, 1296
Hsp92I GRCGYC 1 cut(s) 1122
Hsp92II CATG 5 cut(s) 95, 353, 485, 961, 1078
HspAI GCGC 3 cut(s) 696, 783, 827
Kpn2I TCCGGA 2 cut(s) 247, 1145
KroI GCCGGC 1 cut(s) 1216
KroNI GCCGGC 1 cut(s) 1218
Ksp22I TGATCA 1 cut(s) 427
Kzo9I GATC 4 cut(s) 113, 427, 1148, 1517
LguI GCTCTTC 1 cut(s) 870
LmnI GCTCC 1 cut(s) 197
Lsp1109I GCAGC 3 cut(s) 323, 705, 1050
LweI GCATC 1 cut(s) 707
MaeI CTAG 4 cut(s) 176, 741, 837, 891
MaeII ACGT 5 cut(s) 57, 1043, 1122, 1269, 1296
MaeIII GTNAC 6 cut(s) 151, 659, 858, 952, 1123, 1265
MalI GATC 4 cut(s) 115, 429, 1150, 1519
MbiI CCGCTC 1 cut(s) 752
MboI GATC 4 cut(s) 113, 427, 1148, 1517
MboII GAAGA 4 cut(s) 115, 394, 430, 887
MflI RGATCY 1 cut(s) 1148
MhlI GDGCHC 2 cut(s) 194, 293
MlsI TGGCCA 1 cut(s) 962
MluCI AATT 8 cut(s) 222, 407, 634, 776, 1007, 1098, 1357, 1366
MluNI TGGCCA 1 cut(s) 962
MnlI CCTC 9 cut(s) 220, 244, 259, 262, 315, 639, 683, 717, 997
Mox20I TGGCCA 1 cut(s) 962
MroI TCCGGA 2 cut(s) 247, 1145
MroNI GCCGGC 1 cut(s) 1216
MscI TGGCCA 1 cut(s) 962
MseI TTAA 1 cut(s) 921
MslI CAYNNNNRTG 2 cut(s) 795, 1529
Msp20I TGGCCA 1 cut(s) 962
MspA1I CMGCKG 1 cut(s) 314
MspCI CTTAAG 1 cut(s) 920
MspI CCGG 7 cut(s) 248, 340, 950, 1146, 1217, 1400, 1460
MspR9I CCNGG 2 cut(s) 340, 950
Mva1269I GAATGC 1 cut(s) 931
MvnI CGCG 2 cut(s) 827, 974
MwoI GCNNNNNNNGC 3 cut(s) 494, 877, 1456
NaeI GCCGGC 1 cut(s) 1218
NciI CCSGG 2 cut(s) 340, 950
NcoI CCATGG 1 cut(s) 957
NdeII GATC 4 cut(s) 113, 427, 1148, 1517
NgoMIV GCCGGC 1 cut(s) 1216
NlaIII CATG 5 cut(s) 95, 353, 485, 961, 1078
NlaIV GGNNCC 3 cut(s) 1150, 1425, 1464
NmuCI GTSAC 6 cut(s) 151, 659, 858, 952, 1123, 1265
NotI GCGGCCGC 1 cut(s) 747
NspI RCATGY 1 cut(s) 485
NspV TTCGAA 2 cut(s) 144, 582
OliI CACNNNNGTG 1 cut(s) 1529
PciSI GCTCTTC 1 cut(s) 870
PcsI WCGNNNNNNNCGW 2 cut(s) 552, 1314
PctI GAATGC 1 cut(s) 931
PdiI GCCGGC 1 cut(s) 1218
PfeI GAWTC 4 cut(s) 74, 80, 565, 897
PkrI GCNGC 6 cut(s) 313, 656, 695, 748, 751, 1040
PmaCI CACGTG 1 cut(s) 1044
PmlI CACGTG 1 cut(s) 1044
Ppu21I YACGTR 2 cut(s) 58, 1044
PshAI GACNNNNGTC 1 cut(s) 950
PsiI TTATAA 1 cut(s) 1305
PspCI CACGTG 1 cut(s) 1044
PspEI GGTNACC 1 cut(s) 952
PspFI CCCAGC 1 cut(s) 864
PspN4I GGNNCC 3 cut(s) 1150, 1425, 1464
PspPI GGNCC 3 cut(s) 271, 1423, 1462
PsrI GAACNNNNNNTAC 4 cut(s) 623, 655, 1479, 1511
PstNI CAGNNNCTG 1 cut(s) 672
PsuI RGATCY 1 cut(s) 1148
PvuII CAGCTG 1 cut(s) 314
RsaI GTAC 5 cut(s) 56, 239, 841, 1117, 1346
RsaNI GTAC 5 cut(s) 55, 238, 840, 1116, 1345
RseI CAYNNNNRTG 2 cut(s) 795, 1529
SalI GTCGAC 2 cut(s) 734, 1472
SapI GCTCTTC 1 cut(s) 870
SaqAI TTAA 1 cut(s) 921
SatI GCNGC 6 cut(s) 312, 655, 694, 747, 750, 1039
Sau3AI GATC 4 cut(s) 113, 427, 1148, 1517
Sau96I GGNCC 3 cut(s) 271, 1423, 1462
ScrFI CCNGG 2 cut(s) 340, 950
SduI GDGCHC 2 cut(s) 194, 293
SfaNI GCATC 1 cut(s) 707
SfcI CTRYAG 6 cut(s) 64, 315, 664, 1209, 1383, 1407
SfuI TTCGAA 2 cut(s) 144, 582
SinI GGWCC 3 cut(s) 271, 1423, 1462
SmiMI CAYNNNNRTG 2 cut(s) 795, 1529
SmlI CTYRAG 3 cut(s) 306, 920, 1131
SmoI CTYRAG 3 cut(s) 306, 920, 1131
SpeI ACTAGT 3 cut(s) 175, 740, 836
Sse9I AATT 8 cut(s) 222, 407, 634, 776, 1007, 1098, 1357, 1366
SsiI CCGC 6 cut(s) 654, 746, 750, 966, 1113, 1372
SspMI CTAG 4 cut(s) 176, 741, 837, 891
StyD4I CCNGG 2 cut(s) 338, 948
StyI CCWWGG 2 cut(s) 957, 1254
TaaI ACNGT 7 cut(s) 376, 419, 818, 1003, 1213, 1237, 1310
TaiI ACGT 5 cut(s) 60, 1046, 1125, 1272, 1299
TaqI TCGA 6 cut(s) 144, 441, 582, 701, 735, 1473
TaqII GACCGA 1 cut(s) 1459
TasI AATT 8 cut(s) 222, 407, 634, 776, 1007, 1098, 1357, 1366
TatI WGTACW 2 cut(s) 839, 1344
TauI GCSGC 3 cut(s) 657, 749, 752
TfiI GAWTC 4 cut(s) 74, 80, 565, 897
Tru1I TTAA 1 cut(s) 921
Tru9I TTAA 1 cut(s) 921
TscAI CASTG 5 cut(s) 454, 664, 1006, 1132, 1218
TseFI GTSAC 6 cut(s) 151, 659, 858, 952, 1123, 1265
TseI GCWGC 3 cut(s) 311, 693, 1038
Tsp45I GTSAC 6 cut(s) 151, 659, 858, 952, 1123, 1265
TspDTI ATGAA 3 cut(s) 273, 1096, 1350
TspGWI ACGGA 4 cut(s) 126, 702, 1306, 1405
TspRI CASTG 5 cut(s) 454, 664, 1006, 1132, 1218
Vha464I CTTAAG 1 cut(s) 920
VneI GTGCAC 1 cut(s) 289
VpaK11BI GGWCC 3 cut(s) 271, 1423, 1462
XagI CCTNNNNNAGG 1 cut(s) 643
XapI RAATTY 3 cut(s) 776, 1007, 1098
XceI RCATGY 1 cut(s) 485
XcmI CCANNNNNNNNNTGG 1 cut(s) 166
XmiI GTMKAC 2 cut(s) 735, 1473
XspI CTAG 4 cut(s) 176, 741, 837, 891
ZraI GACGTC 1 cut(s) 1123
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.