MD03G1201500.v1.1

Agglutinin domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0000217
Physical Location & Seq
Forward (+)
27573939 .. 27574859
921 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1201500.v1.1.491

Sequence Viewer

Length: 921 bp
ATGGTCATGCACCTTGTTCGAGCCAGTCTACGTGGACGAACACGATCCTGCTCAAGGCGTCCGTTTTCGTCATGTCCAGCTCCGGAATTATCTTGCTTATGGCGCAATAGCCCGCCCTACGATTCCTGCTTAATTGCAGCAAGTGGGGCGTCCAACAGCGACTTATGCGATGCGTGCTTGATCGTTAATTGGGACTCGCTGTTGGTATTGCCCAAATATATAGCCCTCAAGAGCAACAACGGGAGCTACCTCAGCGCACAGTGGATCGAGCGGCACCCTTACTTGCAGTTCAAATCCGATGACATTGGAGATCCCACGGTGGGGAATGAGGTCTTCCACGCTGGGGATGGAAGCGTTCGCATAAAATCCAATCACCATGGAAAATTCTGGAGGCGAAGCCCTAACTGGATATGGCCAGACTCAGAGCTTCATGATAATGAGAACAACAAGGACTTGTTGTTTTGGCCTGTCAAGCTTGACGGTAAGTTCTTGGCTCTCCGCCGCAACTTGGGTAACAACTTTTTTCTGCAAGAGTCTCAGAGGGGGGGGGGGGAATATAACTGTCTCAACGCTGCCGTCCCCACCATTACCCAAGCTGCACGCTTGGAACTGGAAGAAGTGGTTGTGTCGAGAAGCTTCTACAATGTCAACTTTCACGTCTTGGATGCGAGGGTGTATAACCAAAGAGTCGTCACCATGGCCACCGGAGATGCCATTAACCGAGCAAAGGAACAAAACACCGTAGACATGAAGCTCTCGTACAAGGAGATTAGGAGCAGTACTTGGAACACAAGCGTGTCATTCAAGTCAGGCATCAAGACCACAATGGAAACCGGGGTTCCTTTAATTGCACATGGACAAATCGAAGTGTCGGCCAAGCTGAGGTGGAGACTCTGTGCGTGGTCATTGTGCCACCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aerolysin Agglutinin ETX_MTX2

Protein Analysis

307

Amino Acids

34.64

Weight (kDa)

8.95

Isoelectric Point (pI)

54.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Agglutinin PF07468 71 - 201 6.6e-16 Agglutinin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000217)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g17710 FvH4_6g45590 FvH4_6g45591 FvH4_6g45770 FvH4_6g45770 FvH4_6g46030 FvH4_6g46040 FvH4_7g07020 FvH4_7g07071 FvH4_7g07080
malus_domestica MD01G1104300.v1.1 MD02G1240000.v1.1 MD02G1240200.v1.1 MD03G1201400.v1.1 MD03G1201500.v1.1 MD07G1171100.v1.1
prunus_persica Prupe.2G087400_v2.0.a1 Prupe.2G097500_v2.0.a1 Prupe.2G097700_v2.0.a1 Prupe.2G097800_v2.0.a1 Prupe.3G153100_v2.0.a1 Prupe.3G153300_v2.0.a1 Prupe.3G153600_v2.0.a1 Prupe.5G237200_v2.0.a1
pyrus_communis pycom03g15270 pycom06g20700 pycom07g06030 pycom10g01810 pycom10g01820 pycom10g12090 pycom14g20090 pycom14g20100 pycom14g20110 pycom14g20120
rosa_chinensis RchiOBHm_Chr1g0336971 RchiOBHm_Chr1g0337031 RchiOBHm_Chr2g0163571 RchiOBHm_Chr2g0163591 RchiOBHm_Chr2g0163631 RchiOBHm_Chr2g0163661 RchiOBHm_Chr2g0163671 RchiOBHm_Chr2g0163691 RchiOBHm_Chr2g0163741 RchiOBHm_Chr5g0011821 RchiOBHm_Chr5g0011831 RchiOBHm_Chr5g0011841 RchiOBHm_Chr5g0053101 RchiOBHm_Chr5g0072501
rosa_laevigata RLG00000010009 RLG00000021413 RLG00000029310 RLG00000029318 RLG00000036313
rosa_multiflora Rmu_co7975848.1_g000001 Rmu_co8020886.1_g000001 Rmu_co8136580.1_g000001 Rmu_co8177372.1_g000001 Rmu_co8207014.1_g000001 Rmu_co8232187.1_g000001 Rmu_co8277595.1_g000001 Rmu_co8389219.1_g000001 Rmu_co8427877.1_g000001 Rmu_co8467587.1_g000001 Rmu_sc0001475.1_g000015 Rmu_sc0001475.1_g000016 Rmu_sc0001475.1_g000017 Rmu_sc0003462.1_g000002 Rmu_sc0003462.1_g000003 Rmu_sc0003484.1_g000002 Rmu_sc0004918.1_g000004 Rmu_sc0004918.1_g000006 Rmu_sc0004918.1_g000007 Rmu_sc0004918.1_g000009 Rmu_sc0006596.1_g000003 Rmu_sc0006596.1_g000005 Rmu_sc0006596.1_g000010 Rmu_sc0008676.1_g000021 Rmu_sc0014330.1_g000002 Rmu_sc0016133.1_g000002 Rmu_sc0016133.1_g000003 Rmu_sc0016133.1_g000004 Rmu_sc0026453.1_g000003 Rmu_sc0026453.1_g000004 Rmu_sc0030660.1_g000001
rosa_roxburghii Rroxscaffold_1G00008700 Rroxscaffold_1G00064740 Rroxscaffold_2G00086950 Rroxscaffold_2G00087100 Rroxscaffold_2G00087140 Rroxscaffold_4G00302080 Rroxscaffold_4G00315020 Rroxscaffold_4G00315170 Rroxscaffold_5G00358800
rosa_rugosa Rorug01G0129200 Rorug01G0130600 Rorug01G0130700 Rorug02G0503700 Rorug02G0503800 Rorug02G0503900 Rorug02G0504000 Rorug02G0504300 Rorug05G0268200 Rorug05G0418700 Rorug05G0418800
rosa_samantha Rh1AG151100 Rh1AG151300 Rh1BG118400 Rh1BG120600 Rh1CG141500 Rh1CG142500 Rh1DG157300 Rh1DG158500 Rh2AG570600 Rh2AG570800 Rh2AG571200 Rh2AG571300 Rh2AG571500 Rh2AG571900 Rh2AG572000 Rh2AG572200 Rh2CG552600 Rh2CG552800 Rh2CG553000 Rh2CG553300 Rh2CG553400 Rh2CG553700 Rh5CG100400 Rh5CG100500 Rh5CG100600 Rh5CG100700 Rh5CG519100 Rh5DG086600 Rh5DG086700 Rh5DG086800 Rh5DG086900 Rh5DG087000 Rh5DG087100 Rh5DG367600 Rh5DG507300
rosa_wichuraiana Rw1G012130 Rw1G012240 Rw1G012380 Rw2G047240 Rw2G047260 Rw2G047270 Rw2G047290 Rw5G007940 Rw5G044120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 273
AccBSI CCGCTC 1 cut(s) 271
AccI GTMKAC 2 cut(s) 28, 744
AccIII TCCGGA 1 cut(s) 82
AciI CCGC 4 cut(s) 113, 271, 499, 502
AclWI GGATC 3 cut(s) 39, 272, 305
AcoI YGGCCR 3 cut(s) 413, 699, 873
AcsI RAATTY 1 cut(s) 383
AcyI GRCGYC 2 cut(s) 58, 149
AfaI GTAC 2 cut(s) 761, 781
AfiI CCNNNNNNNGG 7 cut(s) 54, 320, 321, 343, 508, 727, 882
AgsI TTSAA 2 cut(s) 292, 805
AjiI CACGTC 1 cut(s) 658
AleI CACNNNNGTG 1 cut(s) 794
AluBI AGCT 8 cut(s) 80, 246, 427, 475, 596, 636, 754, 880
AluI AGCT 8 cut(s) 80, 246, 427, 475, 596, 636, 754, 880
Alw26I GTCTC 3 cut(s) 540, 569, 883
AlwI GGATC 3 cut(s) 39, 272, 305
Aor13HI TCCGGA 1 cut(s) 82
AoxI GGCC 4 cut(s) 413, 464, 699, 873
ApeKI GCWGC 3 cut(s) 137, 572, 596
ApoI RAATTY 1 cut(s) 383
AspLEI GCGC 2 cut(s) 105, 257
AsuC2I CCSGG 1 cut(s) 835
AsuHPI GGTGA 2 cut(s) 365, 685
BalI TGGCCA 2 cut(s) 415, 701
BanI GGYRCC 1 cut(s) 273
BarI GAAGNNNNNNTAC 2 cut(s) 743, 775
BbsI GAAGAC 1 cut(s) 325
BbvCI CCTCAGC 2 cut(s) 251, 881
BbvI GCAGC 3 cut(s) 149, 559, 583
BccI CCATC 1 cut(s) 341
BceAI ACGGC 1 cut(s) 560
BcgI CGANNNNNNTGC 1 cut(s) 33
BcnI CCSGG 1 cut(s) 835
BcoDI GTCTC 3 cut(s) 540, 569, 883
BisI GCNGC 5 cut(s) 138, 272, 502, 573, 597
BlsI GCNGC 5 cut(s) 139, 273, 503, 574, 598
BmcAI AGTACT 1 cut(s) 781
Bme1390I CCNGG 1 cut(s) 835
BmgBI CACGTC 1 cut(s) 658
BmiI GGNNCC 2 cut(s) 275, 840
BmrFI CCNGG 1 cut(s) 835
BmsI GCATC 4 cut(s) 160, 655, 700, 822
BpiI GAAGAC 1 cut(s) 325
BpmI CTGGAG 1 cut(s) 409
Bpu10I CCTNAGC 2 cut(s) 251, 881
BpuEI CTTGAG 2 cut(s) 37, 212
BpuMI CCSGG 1 cut(s) 835
BsaAI YACGTR 1 cut(s) 32
BsaHI GRCGYC 2 cut(s) 58, 149
BsaJI CCNNGG 4 cut(s) 315, 376, 696, 834
BsaWI WCCGGW 2 cut(s) 82, 704
BsaXI ACNNNNNCTCC 2 cut(s) 880, 910
Bsc4I CCNNNNNNNGG 7 cut(s) 54, 320, 321, 343, 508, 727, 882
Bse1I ACTGG 3 cut(s) 24, 410, 615
BseAI TCCGGA 1 cut(s) 82
BseDI CCNNGG 4 cut(s) 315, 376, 696, 834
BseGI GGATG 2 cut(s) 352, 670
BseLI CCNNNNNNNGG 7 cut(s) 54, 320, 321, 343, 508, 727, 882
BseMII CTCAG 4 cut(s) 265, 435, 551, 872
BseNI ACTGG 3 cut(s) 24, 410, 615
BseXI GCAGC 3 cut(s) 149, 559, 583
BseYI CCCAGC 1 cut(s) 341
BsgI GTGCAG 1 cut(s) 582
BshFI GGCC 4 cut(s) 415, 466, 701, 875
BshNI GGYRCC 1 cut(s) 273
BsiSI CCGG 3 cut(s) 83, 705, 834
BslFI GGGAC 2 cut(s) 206, 563
BslI CCNNNNNNNGG 7 cut(s) 54, 320, 321, 343, 508, 727, 882
BsmAI GTCTC 3 cut(s) 540, 569, 883
BsmFI GGGAC 2 cut(s) 206, 563
BsnI GGCC 4 cut(s) 415, 466, 701, 875
Bsp13I TCCGGA 1 cut(s) 82
Bsp143I GATC 4 cut(s) 44, 180, 264, 310
Bsp19I CCATGG 2 cut(s) 376, 696
BspACI CCGC 4 cut(s) 113, 271, 499, 502
BspANI GGCC 4 cut(s) 415, 466, 701, 875
BspCNI CTCAG 4 cut(s) 264, 434, 550, 873
BspEI TCCGGA 1 cut(s) 82
BspHI TCATGA 1 cut(s) 430
BspLI GGNNCC 2 cut(s) 275, 840
BspPI GGATC 3 cut(s) 39, 272, 305
BspT107I GGYRCC 1 cut(s) 273
BsrBI CCGCTC 1 cut(s) 271
BsrI ACTGG 3 cut(s) 24, 410, 615
BssECI CCNNGG 4 cut(s) 315, 376, 696, 834
BssMI GATC 4 cut(s) 44, 180, 264, 310
BssNI GRCGYC 2 cut(s) 58, 149
BssT1I CCWWGG 2 cut(s) 376, 696
Bst4CI ACNGT 5 cut(s) 261, 319, 482, 563, 742
BstACI GRCGYC 2 cut(s) 58, 149
BstBAI YACGTR 1 cut(s) 32
BstC8I GCNNGC 3 cut(s) 113, 175, 601
BstDEI CTNAG 4 cut(s) 251, 421, 537, 881
BstDSI CCRYGG 3 cut(s) 315, 376, 696
BstENI CCTNNNNNAGG 1 cut(s) 52
BstF5I GGATG 2 cut(s) 352, 670
BstHHI GCGC 2 cut(s) 105, 257
BstKTI GATC 4 cut(s) 47, 183, 267, 313
BstMAI GTCTC 3 cut(s) 540, 569, 883
BstMBI GATC 4 cut(s) 44, 180, 264, 310
BstMWI GCNNNNNNNGC 6 cut(s) 102, 146, 165, 174, 252, 472
BstSCI CCNGG 1 cut(s) 833
BstV1I GCAGC 3 cut(s) 149, 559, 583
BstV2I GAAGAC 1 cut(s) 325
BstX2I RGATCY 1 cut(s) 310
BstYI RGATCY 1 cut(s) 310
BsuRI GGCC 4 cut(s) 415, 466, 701, 875
BtgI CCRYGG 3 cut(s) 315, 376, 696
BtgZI GCGATG 1 cut(s) 183
BtrI CACGTC 1 cut(s) 658
BtsCI GGATG 2 cut(s) 352, 670
BtsIMutI CAGTG 1 cut(s) 266
Cac8I GCNNGC 3 cut(s) 113, 175, 601
CciI TCATGA 1 cut(s) 430
CfoI GCGC 2 cut(s) 105, 257
CseI GACGC 2 cut(s) 47, 138
Csp6I GTAC 2 cut(s) 760, 780
CviAII CATG 7 cut(s) 7, 72, 377, 431, 697, 748, 854
CviQI GTAC 2 cut(s) 760, 780
DdeI CTNAG 4 cut(s) 251, 421, 537, 881
DpnI GATC 4 cut(s) 46, 182, 266, 312
DpnII GATC 4 cut(s) 44, 180, 264, 310
EaeI YGGCCR 3 cut(s) 413, 699, 873
EciI GGCGGA 1 cut(s) 488
Eco130I CCWWGG 2 cut(s) 376, 696
EcoNI CCTNNNNNAGG 1 cut(s) 52
EcoT14I CCWWGG 2 cut(s) 376, 696
ErhI CCWWGG 2 cut(s) 376, 696
FaeI CATG 7 cut(s) 10, 75, 380, 434, 700, 751, 857
FaqI GGGAC 2 cut(s) 206, 563
FatI CATG 7 cut(s) 6, 71, 376, 430, 696, 747, 853
FauI CCCGC 1 cut(s) 120
FblI GTMKAC 2 cut(s) 28, 744
Fnu4HI GCNGC 5 cut(s) 138, 272, 502, 573, 597
FokI GGATG 2 cut(s) 359, 677
Fsp4HI GCNGC 5 cut(s) 138, 272, 502, 573, 597
GlaI GCGC 2 cut(s) 104, 256
GluI GCNGC 5 cut(s) 138, 272, 502, 573, 597
GsaI CCCAGC 1 cut(s) 345
GsuI CTGGAG 1 cut(s) 409
HaeIII GGCC 4 cut(s) 415, 466, 701, 875
HapII CCGG 3 cut(s) 83, 705, 834
HgaI GACGC 2 cut(s) 47, 138
HhaI GCGC 2 cut(s) 105, 257
Hin1I GRCGYC 2 cut(s) 58, 149
Hin1II CATG 7 cut(s) 10, 75, 380, 434, 700, 751, 857
Hin6I GCGC 2 cut(s) 103, 255
HinP1I GCGC 2 cut(s) 103, 255
HincII GTYRAC 1 cut(s) 649
HindII GTYRAC 1 cut(s) 649
HindIII AAGCTT 2 cut(s) 473, 634
HinfI GANTC 6 cut(s) 122, 194, 419, 533, 687, 891
HpaII CCGG 3 cut(s) 83, 705, 834
HphI GGTGA 2 cut(s) 365, 685
Hpy166II GTNNAC 4 cut(s) 29, 35, 649, 745
Hpy188I TCNGA 3 cut(s) 298, 424, 540
Hpy188III TCNNGA 6 cut(s) 83, 229, 388, 431, 630, 817
Hpy8I GTNNAC 4 cut(s) 29, 35, 649, 745
HpyCH4III ACNGT 5 cut(s) 261, 319, 482, 563, 742
HpyCH4IV ACGT 2 cut(s) 31, 657
HpyCH4V TGCA 6 cut(s) 10, 137, 286, 529, 599, 851
HpyF10VI GCNNNNNNNGC 6 cut(s) 102, 146, 165, 174, 252, 472
HpyF3I CTNAG 4 cut(s) 251, 421, 537, 881
HpySE526I ACGT 2 cut(s) 31, 657
Hsp92I GRCGYC 2 cut(s) 58, 149
Hsp92II CATG 7 cut(s) 10, 75, 380, 434, 700, 751, 857
HspAI GCGC 2 cut(s) 103, 255
Kpn2I TCCGGA 1 cut(s) 82
Kzo9I GATC 4 cut(s) 44, 180, 264, 310
LmnI GCTCC 3 cut(s) 85, 243, 774
Lsp1109I GCAGC 3 cut(s) 149, 559, 583
LweI GCATC 4 cut(s) 160, 655, 700, 822
MaeII ACGT 2 cut(s) 31, 657
MaeIII GTNAC 2 cut(s) 512, 691
MalI GATC 4 cut(s) 46, 182, 266, 312
MbiI CCGCTC 1 cut(s) 271
MboI GATC 4 cut(s) 44, 180, 264, 310
MboII GAAGA 2 cut(s) 325, 626
MflI RGATCY 1 cut(s) 310
MlsI TGGCCA 2 cut(s) 415, 701
MluCI AATT 5 cut(s) 86, 132, 187, 383, 846
MluNI TGGCCA 2 cut(s) 415, 701
MlyI GAGTC 5 cut(s) 188, 413, 542, 696, 885
MmeI TCCRAC 1 cut(s) 177
MnlI CCTC 7 cut(s) 236, 260, 322, 384, 534, 663, 876
Mox20I TGGCCA 2 cut(s) 415, 701
MroI TCCGGA 1 cut(s) 82
MscI TGGCCA 2 cut(s) 415, 701
MseI TTAA 4 cut(s) 131, 186, 717, 845
MslI CAYNNNNRTG 2 cut(s) 435, 794
Msp20I TGGCCA 2 cut(s) 415, 701
MspI CCGG 3 cut(s) 83, 705, 834
MspR9I CCNGG 1 cut(s) 835
MwoI GCNNNNNNNGC 6 cut(s) 102, 146, 165, 174, 252, 472
NciI CCSGG 1 cut(s) 835
NcoI CCATGG 2 cut(s) 376, 696
NdeII GATC 4 cut(s) 44, 180, 264, 310
NlaIII CATG 7 cut(s) 10, 75, 380, 434, 700, 751, 857
NlaIV GGNNCC 2 cut(s) 275, 840
NmuCI GTSAC 1 cut(s) 691
OliI CACNNNNGTG 1 cut(s) 794
PagI TCATGA 1 cut(s) 430
PfeI GAWTC 1 cut(s) 122
PkrI GCNGC 5 cut(s) 139, 273, 503, 574, 598
PleI GAGTC 5 cut(s) 188, 413, 541, 695, 885
PpsI GAGTC 5 cut(s) 188, 413, 541, 695, 885
Ppu21I YACGTR 1 cut(s) 32
PspFI CCCAGC 1 cut(s) 341
PspN4I GGNNCC 2 cut(s) 275, 840
PsuI RGATCY 1 cut(s) 310
RsaI GTAC 2 cut(s) 761, 781
RsaNI GTAC 2 cut(s) 760, 780
RseI CAYNNNNRTG 2 cut(s) 435, 794
SaqAI TTAA 4 cut(s) 131, 186, 717, 845
SatI GCNGC 5 cut(s) 138, 272, 502, 573, 597
Sau3AI GATC 4 cut(s) 44, 180, 264, 310
ScaI AGTACT 1 cut(s) 781
SchI GAGTC 5 cut(s) 188, 413, 542, 696, 885
ScrFI CCNGG 1 cut(s) 835
SfaNI GCATC 4 cut(s) 160, 655, 700, 822
SmiMI CAYNNNNRTG 2 cut(s) 435, 794
SmlI CTYRAG 2 cut(s) 52, 227
SmoI CTYRAG 2 cut(s) 52, 227
Sse9I AATT 5 cut(s) 86, 132, 187, 383, 846
SsiI CCGC 4 cut(s) 113, 271, 499, 502
StyD4I CCNGG 1 cut(s) 833
StyI CCWWGG 2 cut(s) 376, 696
TaaI ACNGT 5 cut(s) 261, 319, 482, 563, 742
TaiI ACGT 2 cut(s) 34, 660
TaqI TCGA 4 cut(s) 19, 267, 629, 864
TasI AATT 5 cut(s) 86, 132, 187, 383, 846
TatI WGTACW 1 cut(s) 779
TauI GCSGC 2 cut(s) 274, 504
TfiI GAWTC 1 cut(s) 122
Tru1I TTAA 4 cut(s) 131, 186, 717, 845
Tru9I TTAA 4 cut(s) 131, 186, 717, 845
TscAI CASTG 1 cut(s) 266
TseFI GTSAC 1 cut(s) 691
TseI GCWGC 3 cut(s) 137, 572, 596
Tsp45I GTSAC 1 cut(s) 691
TspDTI ATGAA 2 cut(s) 419, 764
TspGWI ACGGA 1 cut(s) 51
TspRI CASTG 1 cut(s) 266
XagI CCTNNNNNAGG 1 cut(s) 52
XapI RAATTY 1 cut(s) 383
XcmI CCANNNNNNNNNTGG 1 cut(s) 344
XmiI GTMKAC 2 cut(s) 28, 744
ZrmI AGTACT 1 cut(s) 781
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.