Rroxscaffold_5G00358800

Agglutinin domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0000217
Physical Location & Seq
Forward (+)
38924303 .. 38925898
1596 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00358800.1

Sequence Viewer

Length: 1596 bp
ATGTCGTTCCTATTACCACCCACTATTGCGCTCAGTTCAGCCTGGTTCTTCAGTTTCAACGACTCCTACTTGATCTATAGGAATGATTCTGATTCCGAGACGCGTGGCTTTCTCAGAGTCAACGGATCGAATGTCTTTAGCCCATTAGCAAAGTTCGAAGTGGTGACGGCCAATACTGGGACGGGACTGGTACATATCAGATGCTATCATAATAAGAAGTTCCTACGAAGGAGAACTGAAGGTAATGTGAACCTTTCTCCGGAGGCTGATGAACCAGAGGAGGACCAATCCAAATGGTCGTGTACGTTATTTGAGCCTCAAGTAGCTGAAGATGGCAACCCCGACAAAGTCCGGTTGTTCCATGTCCAATCAAATCAATATGCAACGGCATGGGACGTAGGTGGAGCAGTAAGGGACTTGAGATTATATGGAGAGCAGCATAGTCAGAATATATTTGATATCATCGACTTGGAGTCATTGGTGATATTGCCAAAGCATGTGGCCTTCAAAGGCCACACCGGCAATTACCTTCAGCTTGTGGCGGATGCTAAGCTCAGGTTCATATCCACCGATAAGGGGATTCCTGAATCTTGGTTCGAGGTGTCCACTGATGGCCATGGAAGGGTACAAATCAAATCTTACCACGACAATAGCTACTTTTTTAACAGCAGTGACAGGATTTGGTCTGACCCAAGGGTTGCTGCCACCGATGCTAGCACCTTGTTTTGGCCGGTGAAGGTCGACAGTAATAAGGTCGCTCTCAAAAGCAACAACAAGTTTTGCGCCCAAATCAGTGATGCTAATGGAACCGACGCCCTCAATGCAGCCTACATCAGCATTCCAACCAACTCACGCTTCACGCTGGAAGAGCTTGTTCTCTCTAGGAGAATTTACAACACCGACTTTGACCTTAAGAATGCCATAATCTACGGCGAGACCCCGGTCACCATGGCCACCGCAAACGCCAGCAATAACACCTCAGCAGACAACACTGTGGAATTCAAGTTTGCTTACACGGAGAGTAAGAGCAGCACGTGGAGCGCCAGCCACTCGTGGATGGTGGGAGTGAGTGTCACCGTCAGCTTCAAAGTTCCATTCATCGGCGGTACTGAGGTCACTGCTTCCGCAGAATATTCCGGATCATACGAGTGGGGAGAGACAATTACATCTGAAAACACGTTGGAGACCACCTACAAGGTTACAGTGCCGGCAAAAACTTCAATATCTGTGAGCCTGATGGCGACTAAGGGAACTTGCGACGTGCCTTACTCTTATTATCAGCGGGACGTGCTTTATAACGGTAAAACCGTTGTCTATAAGAAAGATGACGGGTTGTACACGGGTGTTAATTCATACAACTTCCGCTATGAGGTTACAACACTGAAAGAGCCAGAAACTAGAGCTGATTTGCCGACCAGACAGCAGAAGTTGCCGGACCCATCGGTCGACCCCTCCATAGAACAAAAGCTACCTCTGCCCGTGCCGTCTGAATCAACCACCAGAGTGCAAAACAAGGTGACCTCGACCACTATCCCGGAACAAGAGTTGTCATTGCTGCAATTAGAAGATGTAGCCAGCTCTGCCATGAAATCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aerolysin Agglutinin ETX_MTX2 Fascin

Protein Analysis

531

Amino Acids

59.29

Weight (kDa)

5.31

Isoelectric Point (pI)

36.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Agglutinin PF07468 18 - 138 3e-19 Agglutinin domain
Aerolysin PF01117 303 - 425 1.1e-09 Aerolysin toxin
ETX_MTX2 PF03318 312 - 424 3.4e-09 Clostridium epsilon toxin ETX/Bacillus mosquitocidal toxin MTX2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000217)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g17710 FvH4_6g45590 FvH4_6g45591 FvH4_6g45770 FvH4_6g45770 FvH4_6g46030 FvH4_6g46040 FvH4_7g07020 FvH4_7g07071 FvH4_7g07080
malus_domestica MD01G1104300.v1.1 MD02G1240000.v1.1 MD02G1240200.v1.1 MD03G1201400.v1.1 MD03G1201500.v1.1 MD07G1171100.v1.1
prunus_persica Prupe.2G087400_v2.0.a1 Prupe.2G097500_v2.0.a1 Prupe.2G097700_v2.0.a1 Prupe.2G097800_v2.0.a1 Prupe.3G153100_v2.0.a1 Prupe.3G153300_v2.0.a1 Prupe.3G153600_v2.0.a1 Prupe.5G237200_v2.0.a1
pyrus_communis pycom03g15270 pycom06g20700 pycom07g06030 pycom10g01810 pycom10g01820 pycom10g12090 pycom14g20090 pycom14g20100 pycom14g20110 pycom14g20120
rosa_chinensis RchiOBHm_Chr1g0336971 RchiOBHm_Chr1g0337031 RchiOBHm_Chr2g0163571 RchiOBHm_Chr2g0163591 RchiOBHm_Chr2g0163631 RchiOBHm_Chr2g0163661 RchiOBHm_Chr2g0163671 RchiOBHm_Chr2g0163691 RchiOBHm_Chr2g0163741 RchiOBHm_Chr5g0011821 RchiOBHm_Chr5g0011831 RchiOBHm_Chr5g0011841 RchiOBHm_Chr5g0053101 RchiOBHm_Chr5g0072501
rosa_laevigata RLG00000010009 RLG00000021413 RLG00000029310 RLG00000029318 RLG00000036313
rosa_multiflora Rmu_co7975848.1_g000001 Rmu_co8020886.1_g000001 Rmu_co8136580.1_g000001 Rmu_co8177372.1_g000001 Rmu_co8207014.1_g000001 Rmu_co8232187.1_g000001 Rmu_co8277595.1_g000001 Rmu_co8389219.1_g000001 Rmu_co8427877.1_g000001 Rmu_co8467587.1_g000001 Rmu_sc0001475.1_g000015 Rmu_sc0001475.1_g000016 Rmu_sc0001475.1_g000017 Rmu_sc0003462.1_g000002 Rmu_sc0003462.1_g000003 Rmu_sc0003484.1_g000002 Rmu_sc0004918.1_g000004 Rmu_sc0004918.1_g000006 Rmu_sc0004918.1_g000007 Rmu_sc0004918.1_g000009 Rmu_sc0006596.1_g000003 Rmu_sc0006596.1_g000005 Rmu_sc0006596.1_g000010 Rmu_sc0008676.1_g000021 Rmu_sc0014330.1_g000002 Rmu_sc0016133.1_g000002 Rmu_sc0016133.1_g000003 Rmu_sc0016133.1_g000004 Rmu_sc0026453.1_g000003 Rmu_sc0026453.1_g000004 Rmu_sc0030660.1_g000001
rosa_roxburghii Rroxscaffold_1G00008700 Rroxscaffold_1G00064740 Rroxscaffold_2G00086950 Rroxscaffold_2G00087100 Rroxscaffold_2G00087140 Rroxscaffold_4G00302080 Rroxscaffold_4G00315020 Rroxscaffold_4G00315170 Rroxscaffold_5G00358800
rosa_rugosa Rorug01G0129200 Rorug01G0130600 Rorug01G0130700 Rorug02G0503700 Rorug02G0503800 Rorug02G0503900 Rorug02G0504000 Rorug02G0504300 Rorug05G0268200 Rorug05G0418700 Rorug05G0418800
rosa_samantha Rh1AG151100 Rh1AG151300 Rh1BG118400 Rh1BG120600 Rh1CG141500 Rh1CG142500 Rh1DG157300 Rh1DG158500 Rh2AG570600 Rh2AG570800 Rh2AG571200 Rh2AG571300 Rh2AG571500 Rh2AG571900 Rh2AG572000 Rh2AG572200 Rh2CG552600 Rh2CG552800 Rh2CG553000 Rh2CG553300 Rh2CG553400 Rh2CG553700 Rh5CG100400 Rh5CG100500 Rh5CG100600 Rh5CG100700 Rh5CG519100 Rh5DG086600 Rh5DG086700 Rh5DG086800 Rh5DG086900 Rh5DG087000 Rh5DG087100 Rh5DG367600 Rh5DG507300
rosa_wichuraiana Rw1G012130 Rw1G012240 Rw1G012380 Rw2G047240 Rw2G047260 Rw2G047270 Rw2G047290 Rw5G007940 Rw5G044120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1296
AasI GACNNNNNNGTC 1 cut(s) 1442
AccI GTMKAC 2 cut(s) 741, 1446
AccII CGCG 1 cut(s) 103
AccIII TCCGGA 2 cut(s) 259, 1136
AciI CCGC 6 cut(s) 542, 957, 1104, 1125, 1282, 1363
AclWI GGATC 2 cut(s) 133, 1147
AcoI YGGCCR 4 cut(s) 168, 613, 728, 951
AcsI RAATTY 2 cut(s) 888, 998
AcuI CTGAAG 4 cut(s) 34, 258, 348, 515
AcvI CACGTG 1 cut(s) 1035
AcyI GRCGYC 1 cut(s) 813
AfaI GTAC 5 cut(s) 192, 304, 627, 1108, 1337
AfiI CCNNNNNNNGG 7 cut(s) 177, 259, 576, 622, 726, 1100, 1369
AflII CTTAAG 1 cut(s) 911
AflIII ACRYGT 2 cut(s) 101, 1176
AgsI TTSAA 5 cut(s) 58, 508, 1003, 1087, 1221
AhdI GACNNNNNGTC 1 cut(s) 472
AjiI CACGTC 2 cut(s) 1261, 1288
AjnI CCWGG 1 cut(s) 41
AjuI GAANNNNNNNTTGG 2 cut(s) 839, 871
AleI CACNNNNGTG 1 cut(s) 1502
AluBI AGCT 9 cut(s) 326, 535, 553, 654, 871, 1083, 1403, 1468, 1578
AluI AGCT 9 cut(s) 326, 535, 553, 654, 871, 1083, 1403, 1468, 1578
Alw26I GTCTC 4 cut(s) 92, 929, 1151, 1178
AlwI GGATC 2 cut(s) 133, 1147
Aor13HI TCCGGA 2 cut(s) 259, 1136
AoxI GGCC 6 cut(s) 168, 501, 511, 613, 728, 951
ApeKI GCWGC 5 cut(s) 436, 701, 824, 1029, 1555
ApoI RAATTY 2 cut(s) 888, 998
AspLEI GCGC 3 cut(s) 31, 785, 1043
AspS9I GGNCC 2 cut(s) 283, 1435
AsuC2I CCSGG 2 cut(s) 941, 1535
AsuHPI GGTGA 6 cut(s) 175, 493, 745, 937, 1066, 1528
AsuII TTCGAA 1 cut(s) 156
AsuNHI GCTAGC 1 cut(s) 713
AvaII GGWCC 2 cut(s) 283, 1435
BaeI ACNNNNGTAYC 2 cut(s) 1098, 1131
BalI TGGCCA 2 cut(s) 615, 953
BauI CACGAG 1 cut(s) 1051
BbrPI CACGTG 1 cut(s) 1035
BbvCI CCTCAGC 1 cut(s) 979
BbvI GCAGC 5 cut(s) 448, 688, 836, 1041, 1542
BccI CCATC 5 cut(s) 326, 605, 1051, 1231, 1447
BceAI ACGGC 4 cut(s) 183, 402, 946, 1468
BciT130I CCWGG 1 cut(s) 43
BcnI CCSGG 2 cut(s) 941, 1535
BcoDI GTCTC 4 cut(s) 92, 929, 1151, 1178
BfaI CTAG 4 cut(s) 714, 882, 1398, 1594
BfmI CTRYAG 1 cut(s) 76
BfoI RGCGCY 1 cut(s) 1044
BfrI CTTAAG 1 cut(s) 911
BglI GCCNNNNNGGC 1 cut(s) 519
BisI GCNGC 5 cut(s) 437, 702, 825, 1030, 1556
BlpI GCTNAGC 1 cut(s) 549
BlsI GCNGC 5 cut(s) 438, 703, 826, 1031, 1557
Bme1390I CCNGG 3 cut(s) 43, 941, 1535
Bme18I GGWCC 2 cut(s) 283, 1435
BmeRI GACNNNNNGTC 1 cut(s) 472
BmgBI CACGTC 2 cut(s) 1261, 1288
BmgT120I GGNCC 2 cut(s) 283, 1435
BmiI GGNNCC 2 cut(s) 808, 1437
BmrFI CCNGG 3 cut(s) 43, 941, 1535
BmrI ACTGGG 1 cut(s) 186
BmsI GCATC 4 cut(s) 191, 535, 700, 787
BmtI GCTAGC 1 cut(s) 717
BmuI ACTGGG 1 cut(s) 186
BoxI GACNNNNGTC 1 cut(s) 941
Bpu10I CCTNAGC 2 cut(s) 554, 979
Bpu1102I GCTNAGC 1 cut(s) 549
Bpu14I TTCGAA 1 cut(s) 156
BpuEI CTTGAG 2 cut(s) 303, 439
BpuMI CCSGG 2 cut(s) 941, 1535
BsaAI YACGTR 1 cut(s) 1035
BsaHI GRCGYC 1 cut(s) 813
BsaI GGTCTC 2 cut(s) 929, 1178
BsaJI CCNNGG 4 cut(s) 616, 692, 939, 948
BsaWI WCCGGW 3 cut(s) 259, 351, 1136
BsaXI ACNNNNNCTCC 2 cut(s) 1056, 1086
Bsc4I CCNNNNNNNGG 7 cut(s) 177, 259, 576, 622, 726, 1100, 1369
Bse118I RCCGGY 3 cut(s) 518, 730, 1207
Bse1I ACTGG 2 cut(s) 181, 192
Bse3DI GCAATG 1 cut(s) 1550
BseAI TCCGGA 2 cut(s) 259, 1136
BseBI CCWGG 1 cut(s) 43
BseDI CCNNGG 4 cut(s) 616, 692, 939, 948
BseGI GGATG 2 cut(s) 550, 1062
BseLI CCNNNNNNNGG 7 cut(s) 177, 259, 576, 622, 726, 1100, 1369
BseMI GCAATG 1 cut(s) 1550
BseMII CTCAG 5 cut(s) 46, 127, 568, 993, 1101
BseNI ACTGG 2 cut(s) 181, 192
BseRI GAGGAG 1 cut(s) 293
BseXI GCAGC 5 cut(s) 448, 688, 836, 1041, 1542
Bsh1236I CGCG 1 cut(s) 103
Bsh1285I CGRYCG 1 cut(s) 1446
BshFI GGCC 6 cut(s) 170, 503, 513, 615, 730, 953
BsiEI CGRYCG 1 cut(s) 1446
BsiSI CCGG 9 cut(s) 260, 352, 519, 731, 941, 1137, 1208, 1433, 1535
BslFI GGGAC 5 cut(s) 193, 198, 407, 428, 1298
BslI CCNNNNNNNGG 7 cut(s) 177, 259, 576, 622, 726, 1100, 1369
BsmAI GTCTC 4 cut(s) 92, 929, 1151, 1178
BsmBI CGTCTC 1 cut(s) 92
BsmFI GGGAC 5 cut(s) 193, 198, 407, 428, 1298
BsmI GAATGC 2 cut(s) 837, 922
BsnI GGCC 6 cut(s) 170, 503, 513, 615, 730, 953
Bso31I GGTCTC 2 cut(s) 929, 1178
Bsp119I TTCGAA 1 cut(s) 156
Bsp13I TCCGGA 2 cut(s) 259, 1136
Bsp1407I TGTACA 1 cut(s) 1335
Bsp143I GATC 3 cut(s) 72, 125, 1139
Bsp1720I GCTNAGC 1 cut(s) 549
Bsp19I CCATGG 2 cut(s) 616, 948
BspACI CCGC 6 cut(s) 542, 957, 1104, 1125, 1282, 1363
BspANI GGCC 6 cut(s) 170, 503, 513, 615, 730, 953
BspCNI CTCAG 5 cut(s) 45, 126, 567, 992, 1102
BspEI TCCGGA 2 cut(s) 259, 1136
BspFNI CGCG 1 cut(s) 103
BspLI GGNNCC 2 cut(s) 808, 1437
BspOI GCTAGC 1 cut(s) 717
BspPI GGATC 2 cut(s) 133, 1147
BspQI GCTCTTC 1 cut(s) 861
BspT104I TTCGAA 1 cut(s) 156
BspTI CTTAAG 1 cut(s) 911
BspTNI GGTCTC 2 cut(s) 929, 1178
BsrDI GCAATG 1 cut(s) 1550
BsrFI RCCGGY 3 cut(s) 518, 730, 1207
BsrGI TGTACA 1 cut(s) 1335
BsrI ACTGG 2 cut(s) 181, 192
BssAI RCCGGY 3 cut(s) 518, 730, 1207
BssECI CCNNGG 4 cut(s) 616, 692, 939, 948
BssMI GATC 3 cut(s) 72, 125, 1139
BssNI GRCGYC 1 cut(s) 813
BssSI CACGAG 1 cut(s) 1051
BssT1I CCWWGG 3 cut(s) 616, 692, 948
Bst2BI CACGAG 1 cut(s) 1051
Bst2UI CCWGG 1 cut(s) 43
Bst4CI ACNGT 6 cut(s) 746, 994, 1078, 1204, 1301, 1309
Bst6I CTCTTC 1 cut(s) 861
BstACI GRCGYC 1 cut(s) 813
BstAFI CTTAAG 1 cut(s) 911
BstAPI GCANNNNNTGC 1 cut(s) 1429
BstAUI TGTACA 1 cut(s) 1335
BstBAI YACGTR 1 cut(s) 1035
BstBI TTCGAA 1 cut(s) 156
BstC8I GCNNGC 5 cut(s) 715, 967, 1045, 1209, 1576
BstDEI CTNAG 7 cut(s) 32, 113, 549, 554, 979, 1110, 1245
BstDSI CCRYGG 2 cut(s) 616, 948
BstEII GGTNACC 2 cut(s) 943, 1516
BstF5I GGATG 2 cut(s) 550, 1062
BstFNI CGCG 1 cut(s) 103
BstH2I RGCGCY 1 cut(s) 1044
BstHHI GCGC 3 cut(s) 31, 785, 1043
BstKTI GATC 3 cut(s) 75, 128, 1142
BstMAI GTCTC 4 cut(s) 92, 929, 1151, 1178
BstMBI GATC 3 cut(s) 72, 125, 1139
BstMCI CGRYCG 1 cut(s) 1446
BstMWI GCNNNNNNNGC 9 cut(s) 519, 710, 821, 868, 1038, 1288, 1429, 1474, 1580
BstNI CCWGG 1 cut(s) 43
BstNSI RCATGY 1 cut(s) 500
BstPAI GACNNNNGTC 1 cut(s) 941
BstPI GGTNACC 2 cut(s) 943, 1516
BstSCI CCNGG 3 cut(s) 41, 939, 1533
BstSFI CTRYAG 1 cut(s) 76
BstUI CGCG 1 cut(s) 103
BstV1I GCAGC 5 cut(s) 448, 688, 836, 1041, 1542
BsuRI GGCC 6 cut(s) 170, 503, 513, 615, 730, 953
BtgI CCRYGG 2 cut(s) 616, 948
BtrI CACGTC 2 cut(s) 1261, 1288
BtsCI GGATG 2 cut(s) 550, 1062
BtsI GCAGTG 2 cut(s) 676, 1116
BtsIMutI CAGTG 7 cut(s) 606, 676, 799, 990, 1116, 1209, 1379
Cac8I GCNNGC 5 cut(s) 715, 967, 1045, 1209, 1576
CfoI GCGC 3 cut(s) 31, 785, 1043
Cfr10I RCCGGY 3 cut(s) 518, 730, 1207
Cfr13I GGNCC 2 cut(s) 283, 1435
CseI GACGC 2 cut(s) 109, 821
Csp6I GTAC 5 cut(s) 191, 303, 626, 1107, 1336
CspCI CAANNNNNGTGG 2 cut(s) 480, 515
CviAII CATG 6 cut(s) 362, 390, 497, 617, 949, 1585
CviQI GTAC 5 cut(s) 191, 303, 626, 1107, 1336
DdeI CTNAG 7 cut(s) 32, 113, 549, 554, 979, 1110, 1245
DpnI GATC 3 cut(s) 74, 127, 1141
DpnII GATC 3 cut(s) 72, 125, 1139
DrdI GACNNNNNNGTC 1 cut(s) 1442
DriI GACNNNNNGTC 1 cut(s) 472
DseDI GACNNNNNNGTC 1 cut(s) 1442
EaeI YGGCCR 4 cut(s) 168, 613, 728, 951
Eam1104I CTCTTC 1 cut(s) 861
Eam1105I GACNNNNNGTC 1 cut(s) 472
EarI CTCTTC 1 cut(s) 861
EciI GGCGGA 1 cut(s) 557
Eco130I CCWWGG 3 cut(s) 616, 692, 948
Eco31I GGTCTC 2 cut(s) 929, 1178
Eco32I GATATC 1 cut(s) 460
Eco47I GGWCC 2 cut(s) 283, 1435
Eco57I CTGAAG 4 cut(s) 34, 258, 348, 515
Eco72I CACGTG 1 cut(s) 1035
Eco91I GGTNACC 2 cut(s) 943, 1516
EcoO65I GGTNACC 2 cut(s) 943, 1516
EcoRI GAATTC 1 cut(s) 998
EcoRII CCWGG 1 cut(s) 41
EcoRV GATATC 1 cut(s) 460
EcoT14I CCWWGG 3 cut(s) 616, 692, 948
ErhI CCWWGG 3 cut(s) 616, 692, 948
Esp3I CGTCTC 1 cut(s) 92
FaeI CATG 6 cut(s) 365, 393, 500, 620, 952, 1588
FaqI GGGAC 5 cut(s) 193, 198, 407, 428, 1298
FatI CATG 6 cut(s) 361, 389, 496, 616, 948, 1584
FauI CCCGC 1 cut(s) 1275
FblI GTMKAC 2 cut(s) 741, 1446
Fnu4HI GCNGC 5 cut(s) 437, 702, 825, 1030, 1556
FokI GGATG 2 cut(s) 557, 1069
Fsp4HI GCNGC 5 cut(s) 437, 702, 825, 1030, 1556
FspBI CTAG 4 cut(s) 714, 882, 1398, 1594
GlaI GCGC 3 cut(s) 30, 784, 1042
GluI GCNGC 5 cut(s) 437, 702, 825, 1030, 1556
HaeII RGCGCY 1 cut(s) 1044
HaeIII GGCC 6 cut(s) 170, 503, 513, 615, 730, 953
HapII CCGG 9 cut(s) 260, 352, 519, 731, 941, 1137, 1208, 1433, 1535
HgaI GACGC 2 cut(s) 109, 821
HhaI GCGC 3 cut(s) 31, 785, 1043
Hin1I GRCGYC 1 cut(s) 813
Hin1II CATG 6 cut(s) 365, 393, 500, 620, 952, 1588
Hin6I GCGC 3 cut(s) 29, 783, 1041
HinP1I GCGC 3 cut(s) 29, 783, 1041
HincII GTYRAC 3 cut(s) 121, 742, 1447
HindII GTYRAC 3 cut(s) 121, 742, 1447
HinfI GANTC 8 cut(s) 62, 86, 92, 117, 473, 580, 587, 1490
HpaII CCGG 9 cut(s) 260, 352, 519, 731, 941, 1137, 1208, 1433, 1535
HphI GGTGA 6 cut(s) 175, 493, 745, 937, 1066, 1528
Hpy166II GTNNAC 7 cut(s) 121, 250, 303, 606, 742, 1338, 1447
Hpy188I TCNGA 8 cut(s) 91, 97, 116, 200, 447, 688, 1171, 1489
Hpy188III TCNNGA 3 cut(s) 260, 584, 1137
Hpy8I GTNNAC 7 cut(s) 121, 250, 303, 606, 742, 1338, 1447
Hpy99I CGWCG 2 cut(s) 815, 1262
HpyAV CCTTC 6 cut(s) 222, 233, 514, 539, 615, 730
HpyCH4III ACNGT 6 cut(s) 746, 994, 1078, 1204, 1301, 1309
HpyCH4IV ACGT 6 cut(s) 305, 396, 1034, 1178, 1260, 1287
HpyCH4V TGCA 4 cut(s) 383, 824, 1507, 1558
HpyF10VI GCNNNNNNNGC 9 cut(s) 519, 710, 821, 868, 1038, 1288, 1429, 1474, 1580
HpyF3I CTNAG 7 cut(s) 32, 113, 549, 554, 979, 1110, 1245
HpySE526I ACGT 6 cut(s) 305, 396, 1034, 1178, 1260, 1287
Hsp92I GRCGYC 1 cut(s) 813
Hsp92II CATG 6 cut(s) 365, 393, 500, 620, 952, 1588
HspAI GCGC 3 cut(s) 29, 783, 1041
Kpn2I TCCGGA 2 cut(s) 259, 1136
KroI GCCGGC 1 cut(s) 1207
KroNI GCCGGC 1 cut(s) 1209
Kzo9I GATC 3 cut(s) 72, 125, 1139
LguI GCTCTTC 1 cut(s) 861
LmnI GCTCC 2 cut(s) 404, 1038
Lsp1109I GCAGC 5 cut(s) 448, 688, 836, 1041, 1542
LweI GCATC 4 cut(s) 191, 535, 700, 787
MaeI CTAG 4 cut(s) 714, 882, 1398, 1594
MaeII ACGT 6 cut(s) 305, 396, 1034, 1178, 1260, 1287
MaeIII GTNAC 8 cut(s) 163, 671, 943, 1072, 1114, 1198, 1372, 1516
MalI GATC 3 cut(s) 74, 127, 1141
MboI GATC 3 cut(s) 72, 125, 1139
MboII GAAGA 4 cut(s) 40, 341, 878, 1577
MlsI TGGCCA 2 cut(s) 615, 953
MluCI AATT 6 cut(s) 523, 888, 998, 1161, 1348, 1559
MluI ACGCGT 1 cut(s) 101
MluNI TGGCCA 2 cut(s) 615, 953
MlyI GAGTC 3 cut(s) 56, 126, 482
MmeI TCCRAC 2 cut(s) 866, 1161
Mox20I TGGCCA 2 cut(s) 615, 953
MroI TCCGGA 2 cut(s) 259, 1136
MroNI GCCGGC 1 cut(s) 1207
MscI TGGCCA 2 cut(s) 615, 953
MseI TTAA 3 cut(s) 663, 912, 1347
MslI CAYNNNNRTG 2 cut(s) 1147, 1502
Msp20I TGGCCA 2 cut(s) 615, 953
MspA1I CMGCKG 1 cut(s) 1282
MspCI CTTAAG 1 cut(s) 911
MspI CCGG 9 cut(s) 260, 352, 519, 731, 941, 1137, 1208, 1433, 1535
MspR9I CCNGG 3 cut(s) 43, 941, 1535
Mva1269I GAATGC 2 cut(s) 837, 922
MvaI CCWGG 1 cut(s) 43
MvnI CGCG 1 cut(s) 103
MwoI GCNNNNNNNGC 9 cut(s) 519, 710, 821, 868, 1038, 1288, 1429, 1474, 1580
NaeI GCCGGC 1 cut(s) 1209
NciI CCSGG 2 cut(s) 941, 1535
NcoI CCATGG 2 cut(s) 616, 948
NdeII GATC 3 cut(s) 72, 125, 1139
NgoMIV GCCGGC 1 cut(s) 1207
NheI GCTAGC 1 cut(s) 713
NlaIII CATG 6 cut(s) 365, 393, 500, 620, 952, 1588
NlaIV GGNNCC 2 cut(s) 808, 1437
NmuCI GTSAC 6 cut(s) 163, 671, 943, 1072, 1114, 1516
NspI RCATGY 1 cut(s) 500
NspV TTCGAA 1 cut(s) 156
OliI CACNNNNGTG 1 cut(s) 1502
PciSI GCTCTTC 1 cut(s) 861
PcsI WCGNNNNNNNCGW 1 cut(s) 1305
PctI GAATGC 2 cut(s) 837, 922
PdiI GCCGGC 1 cut(s) 1209
PfeI GAWTC 5 cut(s) 86, 92, 580, 587, 1490
PflFI GACNNNGTC 1 cut(s) 347
PfoI TCCNGGA 1 cut(s) 1533
PkrI GCNGC 5 cut(s) 438, 703, 826, 1031, 1557
PleI GAGTC 3 cut(s) 56, 125, 481
PmaCI CACGTG 1 cut(s) 1035
PmlI CACGTG 1 cut(s) 1035
PpsI GAGTC 3 cut(s) 56, 125, 481
Ppu21I YACGTR 1 cut(s) 1035
PshAI GACNNNNGTC 1 cut(s) 941
PsiI TTATAA 1 cut(s) 1296
Psp6I CCWGG 1 cut(s) 41
PspCI CACGTG 1 cut(s) 1035
PspEI GGTNACC 2 cut(s) 943, 1516
PspGI CCWGG 1 cut(s) 41
PspN4I GGNNCC 2 cut(s) 808, 1437
PspPI GGNCC 2 cut(s) 283, 1435
PsrI GAACNNNNNNTAC 2 cut(s) 1452, 1484
PsyI GACNNNGTC 1 cut(s) 347
RsaI GTAC 5 cut(s) 192, 304, 627, 1108, 1337
RsaNI GTAC 5 cut(s) 191, 303, 626, 1107, 1336
RseI CAYNNNNRTG 2 cut(s) 1147, 1502
SalI GTCGAC 2 cut(s) 740, 1445
SapI GCTCTTC 1 cut(s) 861
SaqAI TTAA 3 cut(s) 663, 912, 1347
SatI GCNGC 5 cut(s) 437, 702, 825, 1030, 1556
Sau3AI GATC 3 cut(s) 72, 125, 1139
Sau96I GGNCC 2 cut(s) 283, 1435
SchI GAGTC 3 cut(s) 56, 126, 482
ScrFI CCNGG 3 cut(s) 43, 941, 1535
SfaNI GCATC 4 cut(s) 191, 535, 700, 787
SfcI CTRYAG 1 cut(s) 76
SfuI TTCGAA 1 cut(s) 156
SinI GGWCC 2 cut(s) 283, 1435
SmiMI CAYNNNNRTG 2 cut(s) 1147, 1502
SmlI CTYRAG 3 cut(s) 318, 418, 911
SmoI CTYRAG 3 cut(s) 318, 418, 911
Sse9I AATT 6 cut(s) 523, 888, 998, 1161, 1348, 1559
SsiI CCGC 6 cut(s) 542, 957, 1104, 1125, 1282, 1363
SspI AATATT 1 cut(s) 1133
SspMI CTAG 4 cut(s) 714, 882, 1398, 1594
StyD4I CCNGG 3 cut(s) 41, 939, 1533
StyI CCWWGG 3 cut(s) 616, 692, 948
TaaI ACNGT 6 cut(s) 746, 994, 1078, 1204, 1301, 1309
TaiI ACGT 6 cut(s) 308, 399, 1037, 1181, 1263, 1290
TaqI TCGA 7 cut(s) 128, 156, 465, 597, 741, 1446, 1523
TaqII GACCGA 1 cut(s) 1432
TasI AATT 6 cut(s) 523, 888, 998, 1161, 1348, 1559
TatI WGTACW 1 cut(s) 1335
TfiI GAWTC 5 cut(s) 86, 92, 580, 587, 1490
Tru1I TTAA 3 cut(s) 663, 912, 1347
Tru9I TTAA 3 cut(s) 663, 912, 1347
TscAI CASTG 7 cut(s) 613, 676, 799, 997, 1123, 1209, 1386
TseFI GTSAC 6 cut(s) 163, 671, 943, 1072, 1114, 1516
TseI GCWGC 5 cut(s) 436, 701, 824, 1029, 1555
Tsp45I GTSAC 6 cut(s) 163, 671, 943, 1072, 1114, 1516
TspDTI ATGAA 4 cut(s) 285, 550, 1087, 1341
TspGWI ACGGA 2 cut(s) 138, 1031
TspRI CASTG 7 cut(s) 613, 676, 799, 997, 1123, 1209, 1386
Tth111I GACNNNGTC 1 cut(s) 347
Vha464I CTTAAG 1 cut(s) 911
VpaK11BI GGWCC 2 cut(s) 283, 1435
XapI RAATTY 2 cut(s) 888, 998
XceI RCATGY 1 cut(s) 500
XmiI GTMKAC 2 cut(s) 741, 1446
XspI CTAG 4 cut(s) 714, 882, 1398, 1594
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.