Rroxscaffold_2G00087100

Agglutinin domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0000217
Physical Location & Seq
Reverse (-)
9211170 .. 9213254
2085 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00087100.1

Sequence Viewer

Length: 1692 bp
ATGCGGAGCAAGAGTCTAACCACACAAACAAAGATCACTGAACTAGAGGGGGATCTATCATCTCTACATTTCAGTCAGAAAATGTCTTTCCTATTACCACCCACTATTGCTCTGAAATCATCCCACCTCAACGCTTATGTGAACTATAGCGATGATTCAGCTGCTTCCGCGGCCTATGGCTTTCTCCAAGTCAACGGAGGGAATGTCTTCAGCCCGTTAGCAAAGTTCGAAGTCGTGACGGCCAATACTGGGACGGGACTAGTACACATAAGATGCTCACATAATAAAAAGTTCGTGCGATGGAGAACTCAGCATCAAGGGTACCTTTCTCCGGAGGCTGATGAACCAGAGGAGGACCAATCCATATGGTCGTGTACACTGTTTCAGCCTCAAGTAGCTGAAGATGGCGACCCCAAGAAAGTCCTGTTGTTCCATGTCGGATCAAAACAATATGCAAGAGCAATAAACGAAGGTGGAGTTACATACATGAGAACAGATGTCATTTGGAATCATCAGAGTACCTTTTATATCACTGACTTGGAGTCACTGGTGATATTGCCAAAGCATGTAGCCTTCAAAGCCGACAACGACCGCTACCTTCAGCATGCGGACAGTAATCAGCTCAGGTTCATGTCCACCGATAAGGGGATTCCTGAGTCGTGGTTCGAAGTGGACACAGATGGCGATGGAAGGGCGAGAATCAAGGTGTTCAACAGCCTGAGCAACAACCACCTGAGGTTAGGCGCATCCATGGGCAACGCTATTTTTGCTGACGGGAACTCCAACGGCTTCCCGGCTCCCACTTTGTTTTTGCCAGTCAAGGTCGACAGTAGTACGGTCGCTCTCAAATGCTTAGGCAACAACAGATTTTGCACCCGACACACCGGTTTTATACAAGACGGTTTGAGCGCGAACGAAACTAGTACCAGCATTTCAAACTCAGCACGGTTCAGGCTGGAAGAGCTTGTTCTCTCAAGGAGAATCTACAATACCAATTTTGACCTTGCCAATGCCATAATCTACGGCGAGACCCCGGTCACCATGGCCACTGCAAACGCCAGCAACAACAGCTCAGAAGACAACACTGTGGAATTCAAGTTCGCGTACACAGAGAGTAAGAGCAGCACATGGAGCTCCAGCCACTCGTGGATGGTGGGCGTGAGTGTCACCGCCAGCTTAAATATTCCATTCGGACTCGGCGGTGTTGAAACTACATATTCCGGGGCGTATAACGGATCCTATGAATGGGGAGAAACAATTACAACCGAAAACACATTGGAGACTACCTACACCGCCACTGTGCCGGCAAACACTTCTATATCTGTGAGCCTGATGGCGACCAAGGGATATAGCGACGTGCCTTACTCTTATTATCAGCGAGACGTGCTTTATAACGGTGAAACCGTTGTCTATAAGAAGGATGATGGGCTGTACACGGGAATAAATTCATACAACTTCCACTATGTGGTTACAACTCTGAGACAGCCAGAATCTAGAGCTGATTTGTCGACCAGAGAGCAGAAGTTGCCGGACCCATCCGTCGACCCGTCCAAACAAGAAATTGTACCTATGCCTGTACCATCTGCATCAACCACCAGAGTGCAAAACGTGACCTCGACCACCACCCCGGACCAAGGGTTGTCAAAGCTGGGATTAGAAGATGCAGTAGCTCGATCTGCCCTAAAACCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aerolysin Agglutinin ETX_MTX2 Fascin

Protein Analysis

563

Amino Acids

62.04

Weight (kDa)

5.67

Isoelectric Point (pI)

38.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Agglutinin PF07468 32 - 162 7.1e-18 Agglutinin domain
Aerolysin PF01117 332 - 457 9.8e-07 Aerolysin toxin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000217)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g17710 FvH4_6g45590 FvH4_6g45591 FvH4_6g45770 FvH4_6g45770 FvH4_6g46030 FvH4_6g46040 FvH4_7g07020 FvH4_7g07071 FvH4_7g07080
malus_domestica MD01G1104300.v1.1 MD02G1240000.v1.1 MD02G1240200.v1.1 MD03G1201400.v1.1 MD03G1201500.v1.1 MD07G1171100.v1.1
prunus_persica Prupe.2G087400_v2.0.a1 Prupe.2G097500_v2.0.a1 Prupe.2G097700_v2.0.a1 Prupe.2G097800_v2.0.a1 Prupe.3G153100_v2.0.a1 Prupe.3G153300_v2.0.a1 Prupe.3G153600_v2.0.a1 Prupe.5G237200_v2.0.a1
pyrus_communis pycom03g15270 pycom06g20700 pycom07g06030 pycom10g01810 pycom10g01820 pycom10g12090 pycom14g20090 pycom14g20100 pycom14g20110 pycom14g20120
rosa_chinensis RchiOBHm_Chr1g0336971 RchiOBHm_Chr1g0337031 RchiOBHm_Chr2g0163571 RchiOBHm_Chr2g0163591 RchiOBHm_Chr2g0163631 RchiOBHm_Chr2g0163661 RchiOBHm_Chr2g0163671 RchiOBHm_Chr2g0163691 RchiOBHm_Chr2g0163741 RchiOBHm_Chr5g0011821 RchiOBHm_Chr5g0011831 RchiOBHm_Chr5g0011841 RchiOBHm_Chr5g0053101 RchiOBHm_Chr5g0072501
rosa_laevigata RLG00000010009 RLG00000021413 RLG00000029310 RLG00000029318 RLG00000036313
rosa_multiflora Rmu_co7975848.1_g000001 Rmu_co8020886.1_g000001 Rmu_co8136580.1_g000001 Rmu_co8177372.1_g000001 Rmu_co8207014.1_g000001 Rmu_co8232187.1_g000001 Rmu_co8277595.1_g000001 Rmu_co8389219.1_g000001 Rmu_co8427877.1_g000001 Rmu_co8467587.1_g000001 Rmu_sc0001475.1_g000015 Rmu_sc0001475.1_g000016 Rmu_sc0001475.1_g000017 Rmu_sc0003462.1_g000002 Rmu_sc0003462.1_g000003 Rmu_sc0003484.1_g000002 Rmu_sc0004918.1_g000004 Rmu_sc0004918.1_g000006 Rmu_sc0004918.1_g000007 Rmu_sc0004918.1_g000009 Rmu_sc0006596.1_g000003 Rmu_sc0006596.1_g000005 Rmu_sc0006596.1_g000010 Rmu_sc0008676.1_g000021 Rmu_sc0014330.1_g000002 Rmu_sc0016133.1_g000002 Rmu_sc0016133.1_g000003 Rmu_sc0016133.1_g000004 Rmu_sc0026453.1_g000003 Rmu_sc0026453.1_g000004 Rmu_sc0030660.1_g000001
rosa_roxburghii Rroxscaffold_1G00008700 Rroxscaffold_1G00064740 Rroxscaffold_2G00086950 Rroxscaffold_2G00087100 Rroxscaffold_2G00087140 Rroxscaffold_4G00302080 Rroxscaffold_4G00315020 Rroxscaffold_4G00315170 Rroxscaffold_5G00358800
rosa_rugosa Rorug01G0129200 Rorug01G0130600 Rorug01G0130700 Rorug02G0503700 Rorug02G0503800 Rorug02G0503900 Rorug02G0504000 Rorug02G0504300 Rorug05G0268200 Rorug05G0418700 Rorug05G0418800
rosa_samantha Rh1AG151100 Rh1AG151300 Rh1BG118400 Rh1BG120600 Rh1CG141500 Rh1CG142500 Rh1DG157300 Rh1DG158500 Rh2AG570600 Rh2AG570800 Rh2AG571200 Rh2AG571300 Rh2AG571500 Rh2AG571900 Rh2AG572000 Rh2AG572200 Rh2CG552600 Rh2CG552800 Rh2CG553000 Rh2CG553300 Rh2CG553400 Rh2CG553700 Rh5CG100400 Rh5CG100500 Rh5CG100600 Rh5CG100700 Rh5CG519100 Rh5DG086600 Rh5DG086700 Rh5DG086800 Rh5DG086900 Rh5DG087000 Rh5DG087100 Rh5DG367600 Rh5DG507300
rosa_wichuraiana Rw1G012130 Rw1G012240 Rw1G012380 Rw2G047240 Rw2G047260 Rw2G047270 Rw2G047290 Rw5G007940 Rw5G044120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1392
AasI GACNNNNNNGTC 1 cut(s) 1538
Acc65I GGTACC 1 cut(s) 321
AccB1I GGYRCC 1 cut(s) 321
AccB7I CCANNNNNTGG 1 cut(s) 1465
AccI GTMKAC 3 cut(s) 825, 1508, 1542
AccII CGCG 3 cut(s) 170, 911, 1103
AccIII TCCGGA 1 cut(s) 331
AciI CCGC 8 cut(s) 4, 168, 170, 592, 608, 1170, 1200, 1293
AclWI GGATC 4 cut(s) 60, 448, 1230, 1243
AcoI YGGCCR 2 cut(s) 240, 1044
AcsI RAATTY 2 cut(s) 1091, 1444
AcuI CTGAAG 3 cut(s) 193, 420, 584
AdeI CACNNNGTG 1 cut(s) 1465
AfiI CCNNNNNNNGG 6 cut(s) 249, 331, 645, 1245, 1465, 1634
AgeI ACCGGT 1 cut(s) 884
AgsI TTSAA 5 cut(s) 577, 712, 936, 1096, 1208
AhdI GACNNNNNGTC 1 cut(s) 541
AhlI ACTAGT 2 cut(s) 259, 920
AjiI CACGTC 2 cut(s) 1357, 1384
AleI CACNNNNGTG 1 cut(s) 1598
AloI GAACNNNNNNTCC 2 cut(s) 1082, 1114
Alw21I GWGCWC 1 cut(s) 1136
Alw26I GTCTC 4 cut(s) 1022, 1274, 1374, 1474
AlwI GGATC 4 cut(s) 60, 448, 1230, 1243
Aor13HI TCCGGA 1 cut(s) 331
AoxI GGCC 3 cut(s) 171, 240, 1044
ApeKI GCWGC 2 cut(s) 161, 1122
ApoI RAATTY 2 cut(s) 1091, 1444
AsiGI ACCGGT 1 cut(s) 884
Asp700I GAANNNNTTC 2 cut(s) 206, 1444
Asp718I GGTACC 1 cut(s) 321
AspLEI GCGC 2 cut(s) 746, 911
AspS9I GGNCC 3 cut(s) 355, 1531, 1630
AsuC2I CCSGG 4 cut(s) 794, 1034, 1222, 1628
AsuHPI GGTGA 4 cut(s) 562, 1030, 1159, 1409
AsuII TTCGAA 2 cut(s) 228, 666
AvaII GGWCC 3 cut(s) 355, 1531, 1630
AxyI CCTNAGG 1 cut(s) 734
BalI TGGCCA 1 cut(s) 1046
BamHI GGATCC 1 cut(s) 1235
BanI GGYRCC 1 cut(s) 321
BanII GRGCYC 1 cut(s) 1136
BauI CACGAG 1 cut(s) 1144
BbsI GAAGAC 2 cut(s) 199, 1083
Bbv12I GWGCWC 1 cut(s) 1136
BbvI GCAGC 2 cut(s) 148, 1134
BccI CCATC 9 cut(s) 294, 398, 674, 680, 1144, 1327, 1418, 1543, 1588
BceAI ACGGC 3 cut(s) 255, 802, 1039
BcnI CCSGG 4 cut(s) 794, 1034, 1222, 1628
BcoDI GTCTC 4 cut(s) 1022, 1274, 1374, 1474
BcuI ACTAGT 2 cut(s) 259, 920
BfaI CTAG 5 cut(s) 44, 260, 921, 1494, 1690
BfmI CTRYAG 1 cut(s) 145
BisI GCNGC 3 cut(s) 162, 171, 1123
BlsI GCNGC 3 cut(s) 163, 172, 1124
Bme1390I CCNGG 4 cut(s) 794, 1034, 1222, 1628
Bme18I GGWCC 3 cut(s) 355, 1531, 1630
BmeRI GACNNNNNGTC 1 cut(s) 541
BmgBI CACGTC 2 cut(s) 1357, 1384
BmgT120I GGNCC 3 cut(s) 355, 1531, 1630
BmiI GGNNCC 4 cut(s) 323, 798, 1237, 1533
BmrFI CCNGG 4 cut(s) 794, 1034, 1222, 1628
BmrI ACTGGG 1 cut(s) 258
BmsI GCATC 5 cut(s) 263, 322, 755, 1595, 1651
BmuI ACTGGG 1 cut(s) 258
BoxI GACNNNNGTC 1 cut(s) 1034
BpiI GAAGAC 2 cut(s) 199, 1083
BpmI CTGGAG 1 cut(s) 1120
Bpu10I CCTNAGC 3 cut(s) 623, 719, 853
Bpu14I TTCGAA 2 cut(s) 228, 666
BpuEI CTTGAG 2 cut(s) 375, 958
BpuMI CCSGG 4 cut(s) 794, 1034, 1222, 1628
BsaI GGTCTC 1 cut(s) 1022
BsaJI CCNNGG 8 cut(s) 168, 750, 1032, 1041, 1221, 1341, 1626, 1633
BsaWI WCCGGW 2 cut(s) 331, 884
BsaXI ACNNNNNCTCC 5 cut(s) 28, 189, 219, 764, 794
Bsc4I CCNNNNNNNGG 6 cut(s) 249, 331, 645, 1245, 1465, 1634
Bse118I RCCGGY 2 cut(s) 884, 1303
Bse1I ACTGG 3 cut(s) 253, 552, 815
Bse21I CCTNAGG 1 cut(s) 734
BseAI TCCGGA 1 cut(s) 331
BseDI CCNNGG 8 cut(s) 168, 750, 1032, 1041, 1221, 1341, 1626, 1633
BseGI GGATG 5 cut(s) 119, 746, 1155, 1426, 1535
BseLI CCNNNNNNNGG 6 cut(s) 249, 331, 645, 1245, 1465, 1634
BseMII CTCAG 8 cut(s) 323, 637, 645, 710, 725, 954, 1086, 1469
BseNI ACTGG 3 cut(s) 253, 552, 815
BseRI GAGGAG 1 cut(s) 365
BseXI GCAGC 2 cut(s) 148, 1134
BseYI CCCAGC 1 cut(s) 1648
Bsh1236I CGCG 3 cut(s) 170, 911, 1103
Bsh1285I CGRYCG 2 cut(s) 592, 840
BshFI GGCC 3 cut(s) 173, 242, 1046
BshNI GGYRCC 1 cut(s) 321
BshTI ACCGGT 1 cut(s) 884
BsiEI CGRYCG 2 cut(s) 592, 840
BsiHKAI GWGCWC 1 cut(s) 1136
BsiSI CCGG 8 cut(s) 332, 794, 885, 1034, 1221, 1304, 1529, 1628
BslFI GGGAC 2 cut(s) 265, 270
BslI CCNNNNNNNGG 6 cut(s) 249, 331, 645, 1245, 1465, 1634
BsmAI GTCTC 4 cut(s) 1022, 1274, 1374, 1474
BsmBI CGTCTC 1 cut(s) 1374
BsmFI GGGAC 2 cut(s) 265, 270
BsnI GGCC 3 cut(s) 173, 242, 1046
Bso31I GGTCTC 1 cut(s) 1022
Bsp119I TTCGAA 2 cut(s) 228, 666
Bsp1286I GDGCHC 1 cut(s) 1136
Bsp13I TCCGGA 1 cut(s) 331
Bsp1407I TGTACA 2 cut(s) 374, 1431
Bsp143I GATC 5 cut(s) 33, 52, 440, 1235, 1673
Bsp19I CCATGG 2 cut(s) 750, 1041
BspACI CCGC 8 cut(s) 4, 168, 170, 592, 608, 1170, 1200, 1293
BspANI GGCC 3 cut(s) 173, 242, 1046
BspCNI CTCAG 8 cut(s) 322, 636, 646, 711, 726, 953, 1085, 1470
BspEI TCCGGA 1 cut(s) 331
BspFNI CGCG 3 cut(s) 170, 911, 1103
BspLI GGNNCC 4 cut(s) 323, 798, 1237, 1533
BspPI GGATC 4 cut(s) 60, 448, 1230, 1243
BspQI GCTCTTC 1 cut(s) 954
BspT104I TTCGAA 2 cut(s) 228, 666
BspT107I GGYRCC 1 cut(s) 321
BspTNI GGTCTC 1 cut(s) 1022
BsrFI RCCGGY 2 cut(s) 884, 1303
BsrGI TGTACA 2 cut(s) 374, 1431
BsrI ACTGG 3 cut(s) 253, 552, 815
BssAI RCCGGY 2 cut(s) 884, 1303
BssECI CCNNGG 8 cut(s) 168, 750, 1032, 1041, 1221, 1341, 1626, 1633
BssMI GATC 5 cut(s) 33, 52, 440, 1235, 1673
BssSI CACGAG 1 cut(s) 1144
BssT1I CCWWGG 4 cut(s) 750, 1041, 1341, 1633
Bst2BI CACGAG 1 cut(s) 1144
Bst6I CTCTTC 1 cut(s) 954
BstAPI GCANNNNNTGC 1 cut(s) 1525
BstAUI TGTACA 2 cut(s) 374, 1431
BstBI TTCGAA 2 cut(s) 228, 666
BstC8I GCNNGC 4 cut(s) 606, 1060, 1174, 1305
BstDEI CTNAG 9 cut(s) 309, 623, 654, 719, 734, 853, 940, 1072, 1478
BstDSI CCRYGG 3 cut(s) 168, 750, 1041
BstEII GGTNACC 1 cut(s) 1036
BstF5I GGATG 5 cut(s) 119, 746, 1155, 1426, 1535
BstFNI CGCG 3 cut(s) 170, 911, 1103
BstHHI GCGC 2 cut(s) 746, 911
BstKTI GATC 5 cut(s) 36, 55, 443, 1238, 1676
BstMAI GTCTC 4 cut(s) 1022, 1274, 1374, 1474
BstMBI GATC 5 cut(s) 33, 52, 440, 1235, 1673
BstMCI CGRYCG 2 cut(s) 592, 840
BstNSI RCATGY 2 cut(s) 569, 608
BstPAI GACNNNNGTC 1 cut(s) 1034
BstPI GGTNACC 1 cut(s) 1036
BstSCI CCNGG 4 cut(s) 792, 1032, 1220, 1626
BstSFI CTRYAG 1 cut(s) 145
BstUI CGCG 3 cut(s) 170, 911, 1103
BstV1I GCAGC 2 cut(s) 148, 1134
BstV2I GAAGAC 2 cut(s) 199, 1083
BstX2I RGATCY 2 cut(s) 52, 1235
BstYI RGATCY 2 cut(s) 52, 1235
Bsu36I CCTNAGG 1 cut(s) 734
BsuRI GGCC 3 cut(s) 173, 242, 1046
BtgI CCRYGG 3 cut(s) 168, 750, 1041
BtgZI GCGATG 3 cut(s) 165, 313, 699
BtrI CACGTC 2 cut(s) 1357, 1384
BtsCI GGATG 5 cut(s) 119, 746, 1155, 1426, 1535
BtsI GCAGTG 1 cut(s) 1047
BtsIMutI CAGTG 7 cut(s) 36, 377, 531, 545, 1047, 1083, 1296
Cac8I GCNNGC 4 cut(s) 606, 1060, 1174, 1305
CfoI GCGC 2 cut(s) 746, 911
Cfr10I RCCGGY 2 cut(s) 884, 1303
Cfr13I GGNCC 3 cut(s) 355, 1531, 1630
Cfr42I CCGCGG 1 cut(s) 171
CspAI ACCGGT 1 cut(s) 884
CviAII CATG 8 cut(s) 434, 487, 566, 605, 631, 751, 1042, 1128
DdeI CTNAG 9 cut(s) 309, 623, 654, 719, 734, 853, 940, 1072, 1478
DpnI GATC 5 cut(s) 35, 54, 442, 1237, 1675
DpnII GATC 5 cut(s) 33, 52, 440, 1235, 1673
DraIII CACNNNGTG 1 cut(s) 1465
DrdI GACNNNNNNGTC 1 cut(s) 1538
DriI GACNNNNNGTC 1 cut(s) 541
DseDI GACNNNNNNGTC 1 cut(s) 1538
EaeI YGGCCR 2 cut(s) 240, 1044
Eam1104I CTCTTC 1 cut(s) 954
Eam1105I GACNNNNNGTC 1 cut(s) 541
EarI CTCTTC 1 cut(s) 954
Ecl136II GAGCTC 1 cut(s) 1134
Eco130I CCWWGG 4 cut(s) 750, 1041, 1341, 1633
Eco24I GRGCYC 1 cut(s) 1136
Eco31I GGTCTC 1 cut(s) 1022
Eco47I GGWCC 3 cut(s) 355, 1531, 1630
Eco53kI GAGCTC 1 cut(s) 1134
Eco57I CTGAAG 3 cut(s) 193, 420, 584
Eco81I CCTNAGG 1 cut(s) 734
Eco91I GGTNACC 1 cut(s) 1036
EcoICRI GAGCTC 1 cut(s) 1134
EcoO65I GGTNACC 1 cut(s) 1036
EcoRI GAATTC 1 cut(s) 1091
EcoT14I CCWWGG 4 cut(s) 750, 1041, 1341, 1633
EcoT38I GRGCYC 1 cut(s) 1136
ErhI CCWWGG 4 cut(s) 750, 1041, 1341, 1633
Esp3I CGTCTC 1 cut(s) 1374
FaeI CATG 8 cut(s) 437, 490, 569, 608, 634, 754, 1045, 1131
FalI AAGNNNNNCTT 2 cut(s) 309, 341
FaqI GGGAC 2 cut(s) 265, 270
FatI CATG 8 cut(s) 433, 486, 565, 604, 630, 750, 1041, 1127
FauNDI CATATG 1 cut(s) 365
FblI GTMKAC 3 cut(s) 825, 1508, 1542
Fnu4HI GCNGC 3 cut(s) 162, 171, 1123
FokI GGATG 5 cut(s) 106, 733, 1162, 1433, 1522
FriOI GRGCYC 1 cut(s) 1136
Fsp4HI GCNGC 3 cut(s) 162, 171, 1123
FspBI CTAG 5 cut(s) 44, 260, 921, 1494, 1690
GlaI GCGC 2 cut(s) 745, 910
GluI GCNGC 3 cut(s) 162, 171, 1123
GsaI CCCAGC 1 cut(s) 1652
GsuI CTGGAG 1 cut(s) 1120
HaeIII GGCC 3 cut(s) 173, 242, 1046
HapII CCGG 8 cut(s) 332, 794, 885, 1034, 1221, 1304, 1529, 1628
HhaI GCGC 2 cut(s) 746, 911
Hin1II CATG 8 cut(s) 437, 490, 569, 608, 634, 754, 1045, 1131
Hin6I GCGC 2 cut(s) 744, 909
HinP1I GCGC 2 cut(s) 744, 909
HincII GTYRAC 4 cut(s) 193, 826, 1509, 1543
HindII GTYRAC 4 cut(s) 193, 826, 1509, 1543
HpaII CCGG 8 cut(s) 332, 794, 885, 1034, 1221, 1304, 1529, 1628
HphI GGTGA 4 cut(s) 562, 1030, 1159, 1409
Hpy188I TCNGA 7 cut(s) 78, 114, 440, 516, 1075, 1193, 1479
Hpy188III TCNNGA 4 cut(s) 235, 332, 653, 1494
Hpy99I CGWCG 2 cut(s) 1358, 1544
HpyAV CCTTC 5 cut(s) 464, 583, 608, 684, 1411
HpyCH4IV ACGT 3 cut(s) 1356, 1383, 1608
HpyCH4V TGCA 6 cut(s) 455, 873, 1052, 1586, 1603, 1664
HpyF3I CTNAG 9 cut(s) 309, 623, 654, 719, 734, 853, 940, 1072, 1478
HpySE526I ACGT 3 cut(s) 1356, 1383, 1608
Hsp92II CATG 8 cut(s) 437, 490, 569, 608, 634, 754, 1045, 1131
HspAI GCGC 2 cut(s) 744, 909
Kpn2I TCCGGA 1 cut(s) 331
KpnI GGTACC 1 cut(s) 325
KroI GCCGGC 1 cut(s) 1303
KroNI GCCGGC 1 cut(s) 1305
KspI CCGCGG 1 cut(s) 171
Kzo9I GATC 5 cut(s) 33, 52, 440, 1235, 1673
LguI GCTCTTC 1 cut(s) 954
LmnI GCTCC 4 cut(s) 6, 802, 1131, 1139
Lsp1109I GCAGC 2 cut(s) 148, 1134
LweI GCATC 5 cut(s) 263, 322, 755, 1595, 1651
MaeI CTAG 5 cut(s) 44, 260, 921, 1494, 1690
MaeII ACGT 3 cut(s) 1356, 1383, 1608
MaeIII GTNAC 7 cut(s) 235, 478, 543, 1036, 1165, 1468, 1609
MalI GATC 5 cut(s) 35, 54, 442, 1237, 1675
MboI GATC 5 cut(s) 33, 52, 440, 1235, 1673
MboII GAAGA 5 cut(s) 199, 413, 971, 1088, 1670
MflI RGATCY 2 cut(s) 52, 1235
MhlI GDGCHC 1 cut(s) 1136
MlsI TGGCCA 1 cut(s) 1046
MluCI AATT 5 cut(s) 994, 1091, 1257, 1444, 1560
MluNI TGGCCA 1 cut(s) 1046
MlyI GAGTC 4 cut(s) 22, 551, 665, 1188
MmeI TCCRAC 2 cut(s) 418, 807
MnlI CCTC 9 cut(s) 40, 137, 191, 328, 343, 346, 399, 729, 1624
Mox20I TGGCCA 1 cut(s) 1046
MroI TCCGGA 1 cut(s) 331
MroNI GCCGGC 1 cut(s) 1303
MroXI GAANNNNTTC 2 cut(s) 206, 1444
MscI TGGCCA 1 cut(s) 1046
MseI TTAA 1 cut(s) 1178
MslI CAYNNNNRTG 1 cut(s) 1598
Msp20I TGGCCA 1 cut(s) 1046
MspA1I CMGCKG 2 cut(s) 161, 170
MspI CCGG 8 cut(s) 332, 794, 885, 1034, 1221, 1304, 1529, 1628
MspR9I CCNGG 4 cut(s) 794, 1034, 1222, 1628
MvnI CGCG 3 cut(s) 170, 911, 1103
NaeI GCCGGC 1 cut(s) 1305
NciI CCSGG 4 cut(s) 794, 1034, 1222, 1628
NcoI CCATGG 2 cut(s) 750, 1041
NdeI CATATG 1 cut(s) 365
NdeII GATC 5 cut(s) 33, 52, 440, 1235, 1673
NgoMIV GCCGGC 1 cut(s) 1303
NlaIII CATG 8 cut(s) 437, 490, 569, 608, 634, 754, 1045, 1131
NlaIV GGNNCC 4 cut(s) 323, 798, 1237, 1533
NmeAIII GCCGAG 1 cut(s) 1176
NmuCI GTSAC 5 cut(s) 235, 543, 1036, 1165, 1609
NspI RCATGY 2 cut(s) 569, 608
NspV TTCGAA 2 cut(s) 228, 666
OliI CACNNNNGTG 1 cut(s) 1598
PaeI GCATGC 1 cut(s) 608
PciSI GCTCTTC 1 cut(s) 954
PcsI WCGNNNNNNNCGW 1 cut(s) 1401
PdiI GCCGGC 1 cut(s) 1305
PdmI GAANNNNTTC 2 cut(s) 206, 1444
PfeI GAWTC 6 cut(s) 155, 508, 649, 699, 981, 1490
PflMI CCANNNNNTGG 1 cut(s) 1465
PinAI ACCGGT 1 cut(s) 884
PkrI GCNGC 3 cut(s) 163, 172, 1124
PleI GAGTC 4 cut(s) 21, 550, 664, 1188
PpsI GAGTC 4 cut(s) 21, 550, 664, 1188
PshAI GACNNNNGTC 1 cut(s) 1034
PsiI TTATAA 1 cut(s) 1392
Psp124BI GAGCTC 1 cut(s) 1136
PspEI GGTNACC 1 cut(s) 1036
PspFI CCCAGC 1 cut(s) 1648
PspN4I GGNNCC 4 cut(s) 323, 798, 1237, 1533
PspPI GGNCC 3 cut(s) 355, 1531, 1630
PsuI RGATCY 2 cut(s) 52, 1235
PvuII CAGCTG 1 cut(s) 161
RseI CAYNNNNRTG 1 cut(s) 1598
SacI GAGCTC 1 cut(s) 1136
SacII CCGCGG 1 cut(s) 171
SalI GTCGAC 3 cut(s) 824, 1507, 1541
SapI GCTCTTC 1 cut(s) 954
SaqAI TTAA 1 cut(s) 1178
SatI GCNGC 3 cut(s) 162, 171, 1123
Sau3AI GATC 5 cut(s) 33, 52, 440, 1235, 1673
Sau96I GGNCC 3 cut(s) 355, 1531, 1630
SchI GAGTC 4 cut(s) 22, 551, 665, 1188
ScrFI CCNGG 4 cut(s) 794, 1034, 1222, 1628
SduI GDGCHC 1 cut(s) 1136
SfaNI GCATC 5 cut(s) 263, 322, 755, 1595, 1651
SfcI CTRYAG 1 cut(s) 145
Sfr303I CCGCGG 1 cut(s) 171
SfuI TTCGAA 2 cut(s) 228, 666
SgrBI CCGCGG 1 cut(s) 171
SinI GGWCC 3 cut(s) 355, 1531, 1630
SmiMI CAYNNNNRTG 1 cut(s) 1598
SmlI CTYRAG 2 cut(s) 390, 973
SmoI CTYRAG 2 cut(s) 390, 973
SpeI ACTAGT 2 cut(s) 259, 920
SphI GCATGC 1 cut(s) 608
Sse9I AATT 5 cut(s) 994, 1091, 1257, 1444, 1560
SsiI CCGC 8 cut(s) 4, 168, 170, 592, 608, 1170, 1200, 1293
SspI AATATT 1 cut(s) 1183
SspMI CTAG 5 cut(s) 44, 260, 921, 1494, 1690
SstI GAGCTC 1 cut(s) 1136
StyD4I CCNGG 4 cut(s) 792, 1032, 1220, 1626
StyI CCWWGG 4 cut(s) 750, 1041, 1341, 1633
TaiI ACGT 3 cut(s) 1359, 1386, 1611
TaqI TCGA 7 cut(s) 228, 666, 825, 1508, 1542, 1616, 1672
TasI AATT 5 cut(s) 994, 1091, 1257, 1444, 1560
TatI WGTACW 3 cut(s) 262, 374, 1431
TauI GCSGC 1 cut(s) 173
TfiI GAWTC 6 cut(s) 155, 508, 649, 699, 981, 1490
Tru1I TTAA 1 cut(s) 1178
Tru9I TTAA 1 cut(s) 1178
TscAI CASTG 7 cut(s) 43, 384, 538, 552, 1054, 1090, 1303
TseFI GTSAC 5 cut(s) 235, 543, 1036, 1165, 1609
TseI GCWGC 2 cut(s) 161, 1122
Tsp45I GTSAC 5 cut(s) 235, 543, 1036, 1165, 1609
TspDTI ATGAA 4 cut(s) 357, 619, 1257, 1437
TspGWI ACGGA 3 cut(s) 210, 1248, 1528
TspRI CASTG 7 cut(s) 43, 384, 538, 552, 1054, 1090, 1303
Van91I CCANNNNNTGG 1 cut(s) 1465
VpaK11BI GGWCC 3 cut(s) 355, 1531, 1630
XapI RAATTY 2 cut(s) 1091, 1444
XbaI TCTAGA 1 cut(s) 1493
XceI RCATGY 2 cut(s) 569, 608
XmiI GTMKAC 3 cut(s) 825, 1508, 1542
XmnI GAANNNNTTC 2 cut(s) 206, 1444
XspI CTAG 5 cut(s) 44, 260, 921, 1494, 1690
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.