Agglutinin domain
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 185 |
| AccB1I | GGYRCC | 1 cut(s) | 340 |
| AccI | GTMKAC | 1 cut(s) | 290 |
| AfaI | GTAC | 1 cut(s) | 212 |
| AgsI | TTSAA | 2 cut(s) | 121, 128 |
| AjnI | CCWGG | 1 cut(s) | 8 |
| AoxI | GGCC | 1 cut(s) | 55 |
| ApeKI | GCWGC | 1 cut(s) | 5 |
| AspS9I | GGNCC | 1 cut(s) | 47 |
| AsuHPI | GGTGA | 2 cut(s) | 54, 87 |
| AvaII | GGWCC | 1 cut(s) | 47 |
| AxyI | CCTNAGG | 1 cut(s) | 52 |
| BanI | GGYRCC | 1 cut(s) | 340 |
| BbvI | GCAGC | 1 cut(s) | 17 |
| BciT130I | CCWGG | 1 cut(s) | 10 |
| BfaI | CTAG | 2 cut(s) | 44, 189 |
| BisI | GCNGC | 1 cut(s) | 6 |
| BlsI | GCNGC | 1 cut(s) | 7 |
| BmcAI | AGTACT | 1 cut(s) | 212 |
| Bme1390I | CCNGG | 1 cut(s) | 10 |
| Bme18I | GGWCC | 1 cut(s) | 47 |
| BmgT120I | GGNCC | 1 cut(s) | 47 |
| BmiI | GGNNCC | 2 cut(s) | 49, 342 |
| BmrFI | CCNGG | 1 cut(s) | 10 |
| BmsI | GCATC | 1 cut(s) | 139 |
| Bse1I | ACTGG | 2 cut(s) | 218, 344 |
| Bse21I | CCTNAGG | 1 cut(s) | 52 |
| Bse3DI | GCAATG | 1 cut(s) | 312 |
| BseBI | CCWGG | 1 cut(s) | 10 |
| BseMI | GCAATG | 1 cut(s) | 312 |
| BseMII | CTCAG | 1 cut(s) | 66 |
| BseNI | ACTGG | 2 cut(s) | 218, 344 |
| BseRI | GAGGAG | 1 cut(s) | 118 |
| BseXI | GCAGC | 1 cut(s) | 17 |
| BshFI | GGCC | 1 cut(s) | 57 |
| BshNI | GGYRCC | 1 cut(s) | 340 |
| BsnI | GGCC | 1 cut(s) | 57 |
| BspANI | GGCC | 1 cut(s) | 57 |
| BspCNI | CTCAG | 1 cut(s) | 65 |
| BspLI | GGNNCC | 2 cut(s) | 49, 342 |
| BspT107I | GGYRCC | 1 cut(s) | 340 |
| BsrDI | GCAATG | 1 cut(s) | 312 |
| BsrI | ACTGG | 2 cut(s) | 218, 344 |
| Bst2UI | CCWGG | 1 cut(s) | 10 |
| Bst4CI | ACNGT | 1 cut(s) | 116 |
| Bst6I | CTCTTC | 2 cut(s) | 26, 267 |
| BstDEI | CTNAG | 2 cut(s) | 52, 307 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 214 |
| BstNI | CCWGG | 1 cut(s) | 10 |
| BstNSI | RCATGY | 1 cut(s) | 289 |
| BstSCI | CCNGG | 1 cut(s) | 8 |
| BstV1I | GCAGC | 1 cut(s) | 17 |
| Bsu36I | CCTNAGG | 1 cut(s) | 52 |
| BsuRI | GGCC | 1 cut(s) | 57 |
| Cfr13I | GGNCC | 1 cut(s) | 47 |
| Csp6I | GTAC | 1 cut(s) | 211 |
| CviAII | CATG | 2 cut(s) | 250, 286 |
| CviJI | RGCY | 4 cut(s) | 8, 57, 202, 311 |
| CviKI_1 | RGCY | 4 cut(s) | 8, 57, 202, 311 |
| CviQI | GTAC | 1 cut(s) | 211 |
| DdeI | CTNAG | 2 cut(s) | 52, 307 |
| Eam1104I | CTCTTC | 2 cut(s) | 26, 267 |
| EarI | CTCTTC | 2 cut(s) | 26, 267 |
| Eco47I | GGWCC | 1 cut(s) | 47 |
| Eco81I | CCTNAGG | 1 cut(s) | 52 |
| EcoO109I | RGGNCCY | 1 cut(s) | 47 |
| EcoRII | CCWGG | 1 cut(s) | 8 |
| FaeI | CATG | 2 cut(s) | 253, 289 |
| FaiI | YATR | 4 cut(s) | 185, 251, 278, 287 |
| FatI | CATG | 2 cut(s) | 249, 285 |
| FblI | GTMKAC | 1 cut(s) | 290 |
| Fnu4HI | GCNGC | 1 cut(s) | 6 |
| Fsp4HI | GCNGC | 1 cut(s) | 6 |
| FspBI | CTAG | 2 cut(s) | 44, 189 |
| GluI | GCNGC | 1 cut(s) | 6 |
| HaeIII | GGCC | 1 cut(s) | 57 |
| Hin1II | CATG | 2 cut(s) | 253, 289 |
| HincII | GTYRAC | 1 cut(s) | 15 |
| HindII | GTYRAC | 1 cut(s) | 15 |
| HinfI | GANTC | 2 cut(s) | 16, 124 |
| HphI | GGTGA | 2 cut(s) | 54, 87 |
| Hpy166II | GTNNAC | 2 cut(s) | 15, 291 |
| Hpy188III | TCNNGA | 1 cut(s) | 121 |
| Hpy8I | GTNNAC | 2 cut(s) | 15, 291 |
| HpyAV | CCTTC | 1 cut(s) | 230 |
| HpyCH4III | ACNGT | 1 cut(s) | 116 |
| HpyCH4V | TGCA | 1 cut(s) | 152 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 214 |
| HpyF3I | CTNAG | 2 cut(s) | 52, 307 |
| Hsp92II | CATG | 2 cut(s) | 253, 289 |
| LpnPI | CCDG | 5 cut(s) | 22, 39, 199, 210, 216 |
| Lsp1109I | GCAGC | 1 cut(s) | 17 |
| LweI | GCATC | 1 cut(s) | 139 |
| MaeI | CTAG | 2 cut(s) | 44, 189 |
| MboII | GAAGA | 2 cut(s) | 13, 254 |
| MlyI | GAGTC | 1 cut(s) | 10 |
| MmeI | TCCRAC | 1 cut(s) | 311 |
| MnlI | CCTC | 4 cut(s) | 61, 96, 161, 270 |
| MseI | TTAA | 1 cut(s) | 336 |
| MspR9I | CCNGG | 1 cut(s) | 10 |
| MvaI | CCWGG | 1 cut(s) | 10 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 214 |
| NlaIII | CATG | 2 cut(s) | 253, 289 |
| NlaIV | GGNNCC | 2 cut(s) | 49, 342 |
| NspI | RCATGY | 1 cut(s) | 289 |
| PfeI | GAWTC | 1 cut(s) | 124 |
| PkrI | GCNGC | 1 cut(s) | 7 |
| PleI | GAGTC | 1 cut(s) | 10 |
| PpsI | GAGTC | 1 cut(s) | 10 |
| PpuMI | RGGWCCY | 1 cut(s) | 47 |
| PsiI | TTATAA | 1 cut(s) | 185 |
| Psp5II | RGGWCCY | 1 cut(s) | 47 |
| Psp6I | CCWGG | 1 cut(s) | 8 |
| PspGI | CCWGG | 1 cut(s) | 8 |
| PspN4I | GGNNCC | 2 cut(s) | 49, 342 |
| PspPI | GGNCC | 1 cut(s) | 47 |
| PspPPI | RGGWCCY | 1 cut(s) | 47 |
| RsaI | GTAC | 1 cut(s) | 212 |
| RsaNI | GTAC | 1 cut(s) | 211 |
| SaqAI | TTAA | 1 cut(s) | 336 |
| SatI | GCNGC | 1 cut(s) | 6 |
| Sau96I | GGNCC | 1 cut(s) | 47 |
| ScaI | AGTACT | 1 cut(s) | 212 |
| SchI | GAGTC | 1 cut(s) | 10 |
| ScrFI | CCNGG | 1 cut(s) | 10 |
| SetI | ASST | 3 cut(s) | 14, 298, 342 |
| SfaNI | GCATC | 1 cut(s) | 139 |
| SinI | GGWCC | 1 cut(s) | 47 |
| SspMI | CTAG | 2 cut(s) | 44, 189 |
| StyD4I | CCNGG | 1 cut(s) | 8 |
| TaaI | ACNGT | 1 cut(s) | 116 |
| TaqI | TCGA | 1 cut(s) | 349 |
| TatI | WGTACW | 1 cut(s) | 210 |
| TfiI | GAWTC | 1 cut(s) | 124 |
| Tru1I | TTAA | 1 cut(s) | 336 |
| Tru9I | TTAA | 1 cut(s) | 336 |
| TseI | GCWGC | 1 cut(s) | 5 |
| VpaK11BI | GGWCC | 1 cut(s) | 47 |
| XceI | RCATGY | 1 cut(s) | 289 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 210 |
| XmiI | GTMKAC | 1 cut(s) | 290 |
| XspI | CTAG | 2 cut(s) | 44, 189 |
| ZrmI | AGTACT | 1 cut(s) | 212 |