Rw1G012240

Agglutinin domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0000217
Physical Location & Seq
Forward (+)
27366104 .. 27367609
1506 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G012240.1

Sequence Viewer

Length: 1443 bp
ATGGCAGGGCTTCCAAGATTTGTAGTGCTAAAATCCTTGAACCACAAATGCTTGTGTTTAAAAAAAAAAGTTCCAAATGAGCCAGCAGGGTTCATGAAGTGCATTAGAGAACAAGTTGTGAGTCCACAGGCAAAGTTTGAAGTGGAGATGGCAAAGACTGGGAATGGAATGGTGCACATAAGATGCTGCTACAACAACAAGTACTGGGTGACAAACCAGGCTGCCAGGGATGATACAGTTTGGATAGTTGCGTCCGCGGACAAACCAGAGGAAGACCAATCAAAAAGATCCTGCACCTTGTTCAAGCCTAGGTTTGCAGAAGGCCAAACACAAGTCCAATTTATCCACGTCCAACTCTGGATCTCCGTATCCTTGTGGAAGAGTGCTACTACATCCGAGAATGCCTTATATCTAGGAGGACGCGGCGATGTTTTCGAAGTTGTTGACTGGGAATCGCTGGTGATACTTCCCCCACGGGTGGCCTTCAAATCCTTAGAAGATGGCAGTTACCTCTGTTCCCGCTGGATCGAACGACACCCATATCAAAGGTTCGAGTCCAACTTGGATGTTGGAGATCCATTGGTGGCCAAGGAGCGTAGCCCTAACTGGATCTGGGCTGATGCATCATCCGAAAACACGTCCGACGACACTTTGTTTTGGCCTGTCAAAATCCAAGACAATGTCATTGCTCTTCGCAACTTGGGTAACAAAAATTTTTGCGGTGGACTCAGCACCGAATACAAAACCAACTGTCTCAACGCCGCCTACCCTAATATCTGTAAACAGTCAAAGCTGGTGGTGGAAGAGCTTGTTCTTTCAAGGAACATCTACAATATCAATTTCCGACTCTCCGACTCCAGGATCTACCACGAGCAGGTCATAGAAATGGGCACAGCTCTCGCCGTTAATGACAGCCCAAACAGAGACTCCACTATCAGCCTCAAGTTTGCGGAAAAAGATTCCAGGACCCGTAGTTGGAATGGCAGCGTTTCATTGAAGTTGGGTGTCAAAACAACTCTCGAAGTCAAATCTGTTCCACTAATTCTTAACGGAAATATCGAACTTTCGGCTGAGGTTACGACAGCATGCCAGTGGGGAGAAACTACCACAGTTGAACATACCAGGGAAGCCAATTATGCTGTTGTTGTGCCACCACTATCCAAGATGAAGGTGAGTCTCATAGCTTCAAGGGCTTCATGCGATGTGCCCTTCTCGTACAGTCAGCGTGACATTCTCACTGATGGCCGAACCGTTACCAAAACCATGGATGATGGCCTTTTCACTGGGATTAATGCCTACAAATTTGACTACCAAAACCAAGCATCCTACGATGGCCAGCCTCTCTCTGTCTCTAGCCCATGGAGGCTCCCGCGCGCTGTGTACTTTGGGATTTGTGTGATTCTGGTGTTTAGTGTGTTGCTGCCTTTGCTGCATTTGGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aerolysin Agglutinin ETX_MTX2

Protein Analysis

480

Amino Acids

54.02

Weight (kDa)

8.13

Isoelectric Point (pI)

45.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Agglutinin PF07468 3 - 120 6.8e-18 Agglutinin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000217)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g17710 FvH4_6g45590 FvH4_6g45591 FvH4_6g45770 FvH4_6g45770 FvH4_6g46030 FvH4_6g46040 FvH4_7g07020 FvH4_7g07071 FvH4_7g07080
malus_domestica MD01G1104300.v1.1 MD02G1240000.v1.1 MD02G1240200.v1.1 MD03G1201400.v1.1 MD03G1201500.v1.1 MD07G1171100.v1.1
prunus_persica Prupe.2G087400_v2.0.a1 Prupe.2G097500_v2.0.a1 Prupe.2G097700_v2.0.a1 Prupe.2G097800_v2.0.a1 Prupe.3G153100_v2.0.a1 Prupe.3G153300_v2.0.a1 Prupe.3G153600_v2.0.a1 Prupe.5G237200_v2.0.a1
pyrus_communis pycom03g15270 pycom06g20700 pycom07g06030 pycom10g01810 pycom10g01820 pycom10g12090 pycom14g20090 pycom14g20100 pycom14g20110 pycom14g20120
rosa_chinensis RchiOBHm_Chr1g0336971 RchiOBHm_Chr1g0337031 RchiOBHm_Chr2g0163571 RchiOBHm_Chr2g0163591 RchiOBHm_Chr2g0163631 RchiOBHm_Chr2g0163661 RchiOBHm_Chr2g0163671 RchiOBHm_Chr2g0163691 RchiOBHm_Chr2g0163741 RchiOBHm_Chr5g0011821 RchiOBHm_Chr5g0011831 RchiOBHm_Chr5g0011841 RchiOBHm_Chr5g0053101 RchiOBHm_Chr5g0072501
rosa_laevigata RLG00000010009 RLG00000021413 RLG00000029310 RLG00000029318 RLG00000036313
rosa_multiflora Rmu_co7975848.1_g000001 Rmu_co8020886.1_g000001 Rmu_co8136580.1_g000001 Rmu_co8177372.1_g000001 Rmu_co8207014.1_g000001 Rmu_co8232187.1_g000001 Rmu_co8277595.1_g000001 Rmu_co8389219.1_g000001 Rmu_co8427877.1_g000001 Rmu_co8467587.1_g000001 Rmu_sc0001475.1_g000015 Rmu_sc0001475.1_g000016 Rmu_sc0001475.1_g000017 Rmu_sc0003462.1_g000002 Rmu_sc0003462.1_g000003 Rmu_sc0003484.1_g000002 Rmu_sc0004918.1_g000004 Rmu_sc0004918.1_g000006 Rmu_sc0004918.1_g000007 Rmu_sc0004918.1_g000009 Rmu_sc0006596.1_g000003 Rmu_sc0006596.1_g000005 Rmu_sc0006596.1_g000010 Rmu_sc0008676.1_g000021 Rmu_sc0014330.1_g000002 Rmu_sc0016133.1_g000002 Rmu_sc0016133.1_g000003 Rmu_sc0016133.1_g000004 Rmu_sc0026453.1_g000003 Rmu_sc0026453.1_g000004 Rmu_sc0030660.1_g000001
rosa_roxburghii Rroxscaffold_1G00008700 Rroxscaffold_1G00064740 Rroxscaffold_2G00086950 Rroxscaffold_2G00087100 Rroxscaffold_2G00087140 Rroxscaffold_4G00302080 Rroxscaffold_4G00315020 Rroxscaffold_4G00315170 Rroxscaffold_5G00358800
rosa_rugosa Rorug01G0129200 Rorug01G0130600 Rorug01G0130700 Rorug02G0503700 Rorug02G0503800 Rorug02G0503900 Rorug02G0504000 Rorug02G0504300 Rorug05G0268200 Rorug05G0418700 Rorug05G0418800
rosa_samantha Rh1AG151100 Rh1AG151300 Rh1BG118400 Rh1BG120600 Rh1CG141500 Rh1CG142500 Rh1DG157300 Rh1DG158500 Rh2AG570600 Rh2AG570800 Rh2AG571200 Rh2AG571300 Rh2AG571500 Rh2AG571900 Rh2AG572000 Rh2AG572200 Rh2CG552600 Rh2CG552800 Rh2CG553000 Rh2CG553300 Rh2CG553400 Rh2CG553700 Rh5CG100400 Rh5CG100500 Rh5CG100600 Rh5CG100700 Rh5CG519100 Rh5DG086600 Rh5DG086700 Rh5DG086800 Rh5DG086900 Rh5DG087000 Rh5DG087100 Rh5DG367600 Rh5DG507300
rosa_wichuraiana Rw1G012130 Rw1G012240 Rw1G012380 Rw2G047240 Rw2G047260 Rw2G047270 Rw2G047290 Rw5G007940 Rw5G044120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 865
AccII CGCG 4 cut(s) 257, 423, 1372, 1374
AciI CCGC 8 cut(s) 255, 257, 423, 520, 720, 762, 950, 1370
AclWI GGATC 6 cut(s) 282, 368, 533, 569, 617, 869
AcoI YGGCCR 3 cut(s) 585, 1243, 1333
AcsI RAATTY 2 cut(s) 712, 1301
AfaI GTAC 3 cut(s) 203, 1217, 1382
AfiI CCNNNNNNNGG 4 cut(s) 478, 858, 874, 975
AflIII ACRYGT 1 cut(s) 636
AgsI TTSAA 8 cut(s) 40, 140, 304, 487, 819, 997, 1115, 1188
AjiI CACGTC 2 cut(s) 349, 639
AjnI CCWGG 5 cut(s) 216, 224, 857, 962, 1121
AluBI AGCT 4 cut(s) 793, 808, 896, 1184
AluI AGCT 4 cut(s) 793, 808, 896, 1184
Alw21I GWGCWC 1 cut(s) 177
Alw26I GTCTC 4 cut(s) 758, 918, 1181, 1354
Alw44I GTGCAC 1 cut(s) 173
AlwI GGATC 6 cut(s) 282, 368, 533, 569, 617, 869
AoxI GGCC 7 cut(s) 322, 480, 585, 659, 1243, 1273, 1333
ApaLI GTGCAC 1 cut(s) 173
ApeKI GCWGC 5 cut(s) 186, 221, 984, 1420, 1429
ApoI RAATTY 2 cut(s) 712, 1301
AseI ATTAAT 1 cut(s) 1290
AspA2I CCTAGG 1 cut(s) 308
AspLEI GCGC 2 cut(s) 1374, 1376
AspS9I GGNCC 1 cut(s) 966
AsuHPI GGTGA 3 cut(s) 220, 472, 1183
AsuII TTCGAA 1 cut(s) 435
AvaII GGWCC 1 cut(s) 966
AvrII CCTAGG 1 cut(s) 308
BaeGI GKGCMC 3 cut(s) 177, 893, 1209
BalI TGGCCA 2 cut(s) 587, 1335
BauI CACGAG 1 cut(s) 869
BbsI GAAGAC 1 cut(s) 279
Bbv12I GWGCWC 1 cut(s) 177
BbvCI CCTCAGC 1 cut(s) 1071
BbvI GCAGC 5 cut(s) 173, 208, 996, 1407, 1416
BccI CCATC 5 cut(s) 142, 494, 1235, 1265, 1325
BceAI ACGGC 1 cut(s) 887
BcgI CGANNNNNNTGC 2 cut(s) 880, 914
BciT130I CCWGG 5 cut(s) 218, 226, 859, 964, 1123
BciVI GTATCC 1 cut(s) 379
BcoDI GTCTC 4 cut(s) 758, 918, 1181, 1354
BfaI CTAG 3 cut(s) 309, 413, 1353
BfuAI ACCTGC 1 cut(s) 865
BfuI GTATCC 1 cut(s) 379
BisI GCNGC 7 cut(s) 187, 222, 424, 762, 985, 1421, 1430
BlnI CCTAGG 1 cut(s) 308
BlsI GCNGC 7 cut(s) 188, 223, 425, 763, 986, 1422, 1431
BmcAI AGTACT 1 cut(s) 203
Bme1390I CCNGG 5 cut(s) 218, 226, 859, 964, 1123
Bme18I GGWCC 1 cut(s) 966
BmgBI CACGTC 2 cut(s) 349, 639
BmgT120I GGNCC 1 cut(s) 966
BmiI GGNNCC 2 cut(s) 968, 1367
BmrFI CCNGG 5 cut(s) 218, 226, 859, 964, 1123
BmrI ACTGGG 4 cut(s) 168, 214, 457, 1293
BmsI GCATC 4 cut(s) 173, 610, 632, 1331
BmuI ACTGGG 4 cut(s) 168, 214, 457, 1293
BpiI GAAGAC 1 cut(s) 279
BpmI CTGGAG 1 cut(s) 841
Bpu10I CCTNAGC 1 cut(s) 1071
Bpu14I TTCGAA 1 cut(s) 435
BpuEI CTTGAG 1 cut(s) 926
BsaJI CCNNGG 8 cut(s) 225, 255, 308, 473, 588, 1122, 1263, 1358
BsaXI ACNNNNNCTCC 2 cut(s) 911, 941
Bsc4I CCNNNNNNNGG 4 cut(s) 478, 858, 874, 975
Bse1I ACTGG 6 cut(s) 163, 209, 452, 611, 1090, 1288
Bse3DI GCAATG 1 cut(s) 684
BseBI CCWGG 5 cut(s) 218, 226, 859, 964, 1123
BseDI CCNNGG 8 cut(s) 225, 255, 308, 473, 588, 1122, 1263, 1358
BseGI GGATG 6 cut(s) 235, 392, 571, 626, 1273, 1322
BseLI CCNNNNNNNGG 4 cut(s) 478, 858, 874, 975
BseMI GCAATG 1 cut(s) 684
BseMII CTCAG 2 cut(s) 742, 1062
BseNI ACTGG 6 cut(s) 163, 209, 452, 611, 1090, 1288
BsePI GCGCGC 1 cut(s) 1372
BseSI GKGCMC 3 cut(s) 177, 893, 1209
BseXI GCAGC 5 cut(s) 173, 208, 996, 1407, 1416
BsgI GTGCAG 1 cut(s) 277
Bsh1236I CGCG 4 cut(s) 257, 423, 1372, 1374
BshFI GGCC 7 cut(s) 324, 482, 587, 661, 1245, 1275, 1335
BsiHKAI GWGCWC 1 cut(s) 177
BslI CCNNNNNNNGG 4 cut(s) 478, 858, 874, 975
BsmAI GTCTC 4 cut(s) 758, 918, 1181, 1354
BsmI GAATGC 1 cut(s) 406
BsnI GGCC 7 cut(s) 324, 482, 587, 661, 1245, 1275, 1335
Bsp119I TTCGAA 1 cut(s) 435
Bsp1286I GDGCHC 3 cut(s) 177, 893, 1209
Bsp143I GATC 6 cut(s) 287, 360, 525, 574, 609, 861
Bsp19I CCATGG 2 cut(s) 1263, 1358
BspACI CCGC 8 cut(s) 255, 257, 423, 520, 720, 762, 950, 1370
BspANI GGCC 7 cut(s) 324, 482, 587, 661, 1245, 1275, 1335
BspCNI CTCAG 2 cut(s) 741, 1063
BspFNI CGCG 4 cut(s) 257, 423, 1372, 1374
BspHI TCATGA 1 cut(s) 93
BspLI GGNNCC 2 cut(s) 968, 1367
BspMI ACCTGC 1 cut(s) 865
BspPI GGATC 6 cut(s) 282, 368, 533, 569, 617, 869
BspQI GCTCTTC 2 cut(s) 696, 798
BspT104I TTCGAA 1 cut(s) 435
BsrDI GCAATG 1 cut(s) 684
BsrI ACTGG 6 cut(s) 163, 209, 452, 611, 1090, 1288
BssECI CCNNGG 8 cut(s) 225, 255, 308, 473, 588, 1122, 1263, 1358
BssHII GCGCGC 1 cut(s) 1372
BssMI GATC 6 cut(s) 287, 360, 525, 574, 609, 861
BssSI CACGAG 1 cut(s) 869
BssT1I CCWWGG 4 cut(s) 308, 588, 1263, 1358
Bst2BI CACGAG 1 cut(s) 869
Bst2UI CCWGG 5 cut(s) 218, 226, 859, 964, 1123
Bst4CI ACNGT 6 cut(s) 238, 752, 786, 1111, 1220, 1252
Bst6I CTCTTC 3 cut(s) 374, 696, 798
BstBI TTCGAA 1 cut(s) 435
BstC8I GCNNGC 4 cut(s) 84, 1087, 1337, 1374
BstDEI CTNAG 3 cut(s) 493, 728, 1071
BstDSI CCRYGG 4 cut(s) 255, 473, 1263, 1358
BstF5I GGATG 6 cut(s) 235, 392, 571, 626, 1273, 1322
BstFNI CGCG 4 cut(s) 257, 423, 1372, 1374
BstHHI GCGC 2 cut(s) 1374, 1376
BstKTI GATC 6 cut(s) 290, 363, 528, 577, 612, 864
BstMAI GTCTC 4 cut(s) 758, 918, 1181, 1354
BstMBI GATC 6 cut(s) 287, 360, 525, 574, 609, 861
BstMWI GCNNNNNNNGC 4 cut(s) 1136, 1190, 1426, 1429
BstNI CCWGG 5 cut(s) 218, 226, 859, 964, 1123
BstNSI RCATGY 1 cut(s) 1089
BstSCI CCNGG 5 cut(s) 216, 224, 857, 962, 1121
BstSLI GKGCMC 3 cut(s) 177, 893, 1209
BstUI CGCG 4 cut(s) 257, 423, 1372, 1374
BstV1I GCAGC 5 cut(s) 173, 208, 996, 1407, 1416
BstV2I GAAGAC 1 cut(s) 279
BstX2I RGATCY 5 cut(s) 287, 360, 574, 609, 861
BstXI CCANNNNNNTGG 1 cut(s) 1264
BstYI RGATCY 5 cut(s) 287, 360, 574, 609, 861
BsuI GTATCC 1 cut(s) 379
BsuRI GGCC 7 cut(s) 324, 482, 587, 661, 1245, 1275, 1335
BtgI CCRYGG 4 cut(s) 255, 473, 1263, 1358
BtgZI GCGATG 2 cut(s) 441, 1215
BtrI CACGTC 2 cut(s) 349, 639
BtsCI GGATG 6 cut(s) 235, 392, 571, 626, 1273, 1322
BtsIMutI CAGTG 3 cut(s) 1097, 1236, 1281
BveI ACCTGC 1 cut(s) 865
Cac8I GCNNGC 4 cut(s) 84, 1087, 1337, 1374
CciI TCATGA 1 cut(s) 93
CfoI GCGC 2 cut(s) 1374, 1376
Cfr13I GGNCC 1 cut(s) 966
Cfr42I CCGCGG 1 cut(s) 258
CseI GACGC 2 cut(s) 240, 429
Csp6I GTAC 3 cut(s) 202, 1216, 1381
CspCI CAANNNNNGTGG 2 cut(s) 777, 812
CviAII CATG 5 cut(s) 94, 1086, 1197, 1264, 1359
CviQI GTAC 3 cut(s) 202, 1216, 1381
DdeI CTNAG 3 cut(s) 493, 728, 1071
DpnI GATC 6 cut(s) 289, 362, 527, 576, 611, 863
DpnII GATC 6 cut(s) 287, 360, 525, 574, 609, 861
DraI TTTAAA 1 cut(s) 60
EaeI YGGCCR 3 cut(s) 585, 1243, 1333
Eam1104I CTCTTC 3 cut(s) 374, 696, 798
EarI CTCTTC 3 cut(s) 374, 696, 798
Eco130I CCWWGG 4 cut(s) 308, 588, 1263, 1358
Eco47I GGWCC 1 cut(s) 966
EcoO109I RGGNCCY 1 cut(s) 966
EcoRII CCWGG 5 cut(s) 216, 224, 857, 962, 1121
EcoT14I CCWWGG 4 cut(s) 308, 588, 1263, 1358
EcoT22I ATGCAT 1 cut(s) 625
ErhI CCWWGG 4 cut(s) 308, 588, 1263, 1358
FaeI CATG 5 cut(s) 97, 1089, 1200, 1267, 1362
FatI CATG 5 cut(s) 93, 1085, 1196, 1263, 1358
FauI CCCGC 2 cut(s) 527, 1377
Fnu4HI GCNGC 7 cut(s) 187, 222, 424, 762, 985, 1421, 1430
FokI GGATG 6 cut(s) 242, 379, 578, 613, 1280, 1309
Fsp4HI GCNGC 7 cut(s) 187, 222, 424, 762, 985, 1421, 1430
FspBI CTAG 3 cut(s) 309, 413, 1353
GlaI GCGC 2 cut(s) 1373, 1375
GluI GCNGC 7 cut(s) 187, 222, 424, 762, 985, 1421, 1430
GsuI CTGGAG 1 cut(s) 841
HaeIII GGCC 7 cut(s) 324, 482, 587, 661, 1245, 1275, 1335
HgaI GACGC 2 cut(s) 240, 429
HhaI GCGC 2 cut(s) 1374, 1376
Hin1II CATG 5 cut(s) 97, 1089, 1200, 1267, 1362
Hin6I GCGC 2 cut(s) 1372, 1374
HinP1I GCGC 2 cut(s) 1372, 1374
HincII GTYRAC 1 cut(s) 445
HindII GTYRAC 1 cut(s) 445
HphI GGTGA 3 cut(s) 220, 472, 1183
Hpy166II GTNNAC 6 cut(s) 125, 175, 445, 725, 782, 1381
Hpy188I TCNGA 5 cut(s) 397, 631, 643, 845, 853
Hpy188III TCNNGA 3 cut(s) 94, 358, 1019
Hpy8I GTNNAC 6 cut(s) 125, 175, 445, 725, 782, 1381
Hpy99I CGWCG 1 cut(s) 647
HpyAV CCTTC 4 cut(s) 314, 493, 1162, 1219
HpyCH4III ACNGT 6 cut(s) 238, 752, 786, 1111, 1220, 1252
HpyCH4IV ACGT 2 cut(s) 348, 638
HpyCH4V TGCA 6 cut(s) 102, 175, 294, 317, 623, 1432
HpyF10VI GCNNNNNNNGC 4 cut(s) 1136, 1190, 1426, 1429
HpyF3I CTNAG 3 cut(s) 493, 728, 1071
HpySE526I ACGT 2 cut(s) 348, 638
Hsp92II CATG 5 cut(s) 97, 1089, 1200, 1267, 1362
HspAI GCGC 2 cut(s) 1372, 1374
KspI CCGCGG 1 cut(s) 258
Kzo9I GATC 6 cut(s) 287, 360, 525, 574, 609, 861
LguI GCTCTTC 2 cut(s) 696, 798
LmnI GCTCC 2 cut(s) 592, 1371
Lsp1109I GCAGC 5 cut(s) 173, 208, 996, 1407, 1416
LweI GCATC 4 cut(s) 173, 610, 632, 1331
MaeI CTAG 3 cut(s) 309, 413, 1353
MaeII ACGT 2 cut(s) 348, 638
MaeIII GTNAC 6 cut(s) 208, 506, 704, 1075, 1226, 1252
MalI GATC 6 cut(s) 289, 362, 527, 576, 611, 863
MboI GATC 6 cut(s) 287, 360, 525, 574, 609, 861
MboII GAAGA 5 cut(s) 284, 391, 509, 683, 815
MflI RGATCY 5 cut(s) 287, 360, 574, 609, 861
MhlI GDGCHC 3 cut(s) 177, 893, 1209
MlsI TGGCCA 2 cut(s) 587, 1335
MluCI AATT 6 cut(s) 338, 712, 838, 1041, 1132, 1301
MluNI TGGCCA 2 cut(s) 587, 1335
MlyI GAGTC 7 cut(s) 130, 563, 720, 840, 848, 920, 1183
MmeI TCCRAC 7 cut(s) 376, 550, 582, 666, 868, 876, 956
MnlI CCTC 7 cut(s) 262, 410, 521, 950, 1066, 1350, 1356
Mox20I TGGCCA 2 cut(s) 587, 1335
Mph1103I ATGCAT 1 cut(s) 625
MscI TGGCCA 2 cut(s) 587, 1335
MseI TTAA 4 cut(s) 59, 906, 1047, 1290
MslI CAYNNNNRTG 2 cut(s) 884, 1090
Msp20I TGGCCA 2 cut(s) 587, 1335
MspA1I CMGCKG 2 cut(s) 257, 522
MspR9I CCNGG 5 cut(s) 218, 226, 859, 964, 1123
Mva1269I GAATGC 1 cut(s) 406
MvaI CCWGG 5 cut(s) 218, 226, 859, 964, 1123
MvnI CGCG 4 cut(s) 257, 423, 1372, 1374
MwoI GCNNNNNNNGC 4 cut(s) 1136, 1190, 1426, 1429
NcoI CCATGG 2 cut(s) 1263, 1358
NdeII GATC 6 cut(s) 287, 360, 525, 574, 609, 861
NlaIII CATG 5 cut(s) 97, 1089, 1200, 1267, 1362
NlaIV GGNNCC 2 cut(s) 968, 1367
NmuCI GTSAC 2 cut(s) 208, 1226
NsiI ATGCAT 1 cut(s) 625
NspI RCATGY 1 cut(s) 1089
NspV TTCGAA 1 cut(s) 435
PaeI GCATGC 1 cut(s) 1089
PagI TCATGA 1 cut(s) 93
PauI GCGCGC 1 cut(s) 1372
PciSI GCTCTTC 2 cut(s) 696, 798
PcsI WCGNNNNNNNCGW 1 cut(s) 1056
PctI GAATGC 1 cut(s) 406
PfeI GAWTC 3 cut(s) 452, 959, 1399
PflFI GACNNNGTC 1 cut(s) 680
PfoI TCCNGGA 2 cut(s) 857, 962
PkrI GCNGC 7 cut(s) 188, 223, 425, 763, 986, 1422, 1431
PleI GAGTC 7 cut(s) 129, 562, 720, 840, 848, 920, 1182
PpsI GAGTC 7 cut(s) 129, 562, 720, 840, 848, 920, 1182
PpuMI RGGWCCY 1 cut(s) 966
PshBI ATTAAT 1 cut(s) 1290
Psp5II RGGWCCY 1 cut(s) 966
Psp6I CCWGG 5 cut(s) 216, 224, 857, 962, 1121
PspGI CCWGG 5 cut(s) 216, 224, 857, 962, 1121
PspN4I GGNNCC 2 cut(s) 968, 1367
PspPI GGNCC 1 cut(s) 966
PspPPI RGGWCCY 1 cut(s) 966
PsuI RGATCY 5 cut(s) 287, 360, 574, 609, 861
PsyI GACNNNGTC 1 cut(s) 680
PteI GCGCGC 1 cut(s) 1372
RsaI GTAC 3 cut(s) 203, 1217, 1382
RsaNI GTAC 3 cut(s) 202, 1216, 1381
RseI CAYNNNNRTG 2 cut(s) 884, 1090
SacII CCGCGG 1 cut(s) 258
SapI GCTCTTC 2 cut(s) 696, 798
SaqAI TTAA 4 cut(s) 59, 906, 1047, 1290
SatI GCNGC 7 cut(s) 187, 222, 424, 762, 985, 1421, 1430
Sau3AI GATC 6 cut(s) 287, 360, 525, 574, 609, 861
Sau96I GGNCC 1 cut(s) 966
ScaI AGTACT 1 cut(s) 203
SchI GAGTC 7 cut(s) 130, 563, 720, 840, 848, 920, 1183
ScrFI CCNGG 5 cut(s) 218, 226, 859, 964, 1123
SduI GDGCHC 3 cut(s) 177, 893, 1209
SfaNI GCATC 4 cut(s) 173, 610, 632, 1331
Sfr303I CCGCGG 1 cut(s) 258
SfuI TTCGAA 1 cut(s) 435
SgrBI CCGCGG 1 cut(s) 258
SinI GGWCC 1 cut(s) 966
SmiMI CAYNNNNRTG 2 cut(s) 884, 1090
SmlI CTYRAG 1 cut(s) 941
SmoI CTYRAG 1 cut(s) 941
SphI GCATGC 1 cut(s) 1089
Sse9I AATT 6 cut(s) 338, 712, 838, 1041, 1132, 1301
SsiI CCGC 8 cut(s) 255, 257, 423, 520, 720, 762, 950, 1370
SspMI CTAG 3 cut(s) 309, 413, 1353
StyD4I CCNGG 5 cut(s) 216, 224, 857, 962, 1121
StyI CCWWGG 4 cut(s) 308, 588, 1263, 1358
TaaI ACNGT 6 cut(s) 238, 752, 786, 1111, 1220, 1252
TaiI ACGT 2 cut(s) 351, 641
TaqI TCGA 5 cut(s) 435, 528, 552, 1020, 1059
TasI AATT 6 cut(s) 338, 712, 838, 1041, 1132, 1301
TatI WGTACW 2 cut(s) 201, 1380
TauI GCSGC 2 cut(s) 426, 764
TfiI GAWTC 3 cut(s) 452, 959, 1399
Tru1I TTAA 4 cut(s) 59, 906, 1047, 1290
Tru9I TTAA 4 cut(s) 59, 906, 1047, 1290
TscAI CASTG 3 cut(s) 1097, 1243, 1288
TseFI GTSAC 2 cut(s) 208, 1226
TseI GCWGC 5 cut(s) 186, 221, 984, 1420, 1429
Tsp45I GTSAC 2 cut(s) 208, 1226
TspDTI ATGAA 5 cut(s) 82, 110, 981, 1181, 1185
TspGWI ACGGA 2 cut(s) 355, 1065
TspRI CASTG 3 cut(s) 1097, 1243, 1288
Tth111I GACNNNGTC 1 cut(s) 680
VneI GTGCAC 1 cut(s) 173
VpaK11BI GGWCC 1 cut(s) 966
VspI ATTAAT 1 cut(s) 1290
XapI RAATTY 2 cut(s) 712, 1301
XceI RCATGY 1 cut(s) 1089
XmaJI CCTAGG 1 cut(s) 308
XspI CTAG 3 cut(s) 309, 413, 1353
ZrmI AGTACT 1 cut(s) 203
Zsp2I ATGCAT 1 cut(s) 625
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.