Agglutinin domain
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccI | GTMKAC | 1 cut(s) | 834 |
| AccII | CGCG | 1 cut(s) | 322 |
| AciI | CCGC | 5 cut(s) | 820, 959, 1240, 1381, 1695 |
| AclWI | GGATC | 6 cut(s) | 320, 398, 500, 545, 1553, 1789 |
| AcoI | YGGCCR | 1 cut(s) | 1887 |
| AcsI | RAATTY | 3 cut(s) | 155, 1370, 1594 |
| AcvI | CACGTG | 1 cut(s) | 1656 |
| AdeI | CACNNNGTG | 1 cut(s) | 1040 |
| AfaI | GTAC | 8 cut(s) | 364, 916, 937, 979, 1021, 1172, 1942, 2018 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 1297, 1387, 1733 |
| AflIII | ACRYGT | 5 cut(s) | 1044, 1090, 1158, 1335, 1655 |
| AgeI | ACCGGT | 1 cut(s) | 345 |
| AgsI | TTSAA | 8 cut(s) | 284, 530, 593, 1487, 1764, 1873, 1999, 2014 |
| AhlI | ACTAGT | 1 cut(s) | 232 |
| AjiI | CACGTC | 2 cut(s) | 1045, 1852 |
| AjnI | CCWGG | 1 cut(s) | 225 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 1563, 1595 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 16, 48 |
| Alw21I | GWGCWC | 5 cut(s) | 359, 525, 727, 733, 1655 |
| Alw26I | GTCTC | 2 cut(s) | 197, 1805 |
| AlwI | GGATC | 6 cut(s) | 320, 398, 500, 545, 1553, 1789 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 509 |
| AoxI | GGCC | 2 cut(s) | 1587, 1887 |
| ApeKI | GCWGC | 5 cut(s) | 249, 277, 1140, 1202, 1929 |
| ApoI | RAATTY | 3 cut(s) | 155, 1370, 1594 |
| AseI | ATTAAT | 3 cut(s) | 18, 114, 1059 |
| AsiGI | ACCGGT | 1 cut(s) | 345 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 588 |
| AspLEI | GCGC | 2 cut(s) | 322, 1207 |
| AspS9I | GGNCC | 2 cut(s) | 348, 1644 |
| AsuC2I | CCSGG | 1 cut(s) | 1284 |
| AsuHPI | GGTGA | 9 cut(s) | 376, 461, 861, 953, 1087, 1298, 1444, 1466, 1520 |
| AsuII | TTCGAA | 1 cut(s) | 195 |
| AvaII | GGWCC | 2 cut(s) | 348, 1644 |
| AxyI | CCTNAGG | 1 cut(s) | 630 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 1924, 1957 |
| BalI | TGGCCA | 1 cut(s) | 1889 |
| BanII | GRGCYC | 1 cut(s) | 359 |
| BauI | CACGAG | 1 cut(s) | 726 |
| BbrPI | CACGTG | 1 cut(s) | 1656 |
| BbsI | GAAGAC | 3 cut(s) | 348, 776, 1111 |
| Bbv12I | GWGCWC | 5 cut(s) | 359, 525, 727, 733, 1655 |
| BbvCI | CCTCAGC | 1 cut(s) | 1124 |
| BbvI | GCAGC | 5 cut(s) | 236, 264, 1152, 1214, 1941 |
| BccI | CCATC | 4 cut(s) | 440, 446, 1187, 1973 |
| BceAI | ACGGC | 2 cut(s) | 222, 1574 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 416, 450 |
| BciT130I | CCWGG | 1 cut(s) | 227 |
| BclI | TGATCA | 4 cut(s) | 130, 304, 964, 1006 |
| BcnI | CCSGG | 1 cut(s) | 1284 |
| BcoDI | GTCTC | 2 cut(s) | 197, 1805 |
| BcuI | ACTAGT | 1 cut(s) | 232 |
| BfaI | CTAG | 2 cut(s) | 233, 1542 |
| BisI | GCNGC | 6 cut(s) | 250, 278, 1141, 1203, 1696, 1930 |
| BlsI | GCNGC | 6 cut(s) | 251, 279, 1142, 1204, 1697, 1931 |
| BmcAI | AGTACT | 1 cut(s) | 916 |
| Bme1390I | CCNGG | 2 cut(s) | 227, 1284 |
| Bme18I | GGWCC | 2 cut(s) | 348, 1644 |
| BmgBI | CACGTC | 2 cut(s) | 1045, 1852 |
| BmgT120I | GGNCC | 2 cut(s) | 348, 1644 |
| BmiI | GGNNCC | 2 cut(s) | 138, 179 |
| BmrFI | CCNGG | 2 cut(s) | 227, 1284 |
| BmrI | ACTGGG | 3 cut(s) | 277, 1306, 1623 |
| BmsI | GCATC | 6 cut(s) | 236, 424, 529, 610, 1245, 1657 |
| BmuI | ACTGGG | 3 cut(s) | 277, 1306, 1623 |
| BpiI | GAAGAC | 3 cut(s) | 348, 776, 1111 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 1107, 1139 |
| BpmI | CTGGAG | 1 cut(s) | 183 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1124 |
| Bpu14I | TTCGAA | 1 cut(s) | 195 |
| BpuEI | CTTGAG | 1 cut(s) | 118 |
| BpuMI | CCSGG | 1 cut(s) | 1284 |
| Bsa29I | ATCGAT | 1 cut(s) | 492 |
| BsaAI | YACGTR | 2 cut(s) | 811, 1656 |
| BsaBI | GATNNNNATC | 2 cut(s) | 498, 600 |
| BsaJI | CCNNGG | 2 cut(s) | 141, 226 |
| BsaWI | WCCGGW | 3 cut(s) | 345, 1726, 1825 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 156, 186, 1575, 1605 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 1297, 1387, 1733 |
| Bse118I | RCCGGY | 1 cut(s) | 345 |
| Bse1I | ACTGG | 3 cut(s) | 272, 1301, 1618 |
| Bse21I | CCTNAGG | 1 cut(s) | 630 |
| Bse3DI | GCAATG | 1 cut(s) | 1866 |
| Bse8I | GATNNNNATC | 2 cut(s) | 498, 600 |
| BseBI | CCWGG | 1 cut(s) | 227 |
| BseCI | ATCGAT | 1 cut(s) | 492 |
| BseDI | CCNNGG | 2 cut(s) | 141, 226 |
| BseGI | GGATG | 8 cut(s) | 499, 624, 733, 904, 967, 1236, 1623, 1981 |
| BseJI | GATNNNNATC | 2 cut(s) | 498, 600 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 1297, 1387, 1733 |
| BseMI | GCAATG | 1 cut(s) | 1866 |
| BseMII | CTCAG | 3 cut(s) | 621, 1138, 1469 |
| BseNI | ACTGG | 3 cut(s) | 272, 1301, 1618 |
| BseRI | GAGGAG | 1 cut(s) | 689 |
| BseXI | GCAGC | 5 cut(s) | 236, 264, 1152, 1214, 1941 |
| BsgI | GTGCAG | 2 cut(s) | 1850, 1861 |
| Bsh1236I | CGCG | 1 cut(s) | 322 |
| BshFI | GGCC | 2 cut(s) | 1589, 1889 |
| BshTI | ACCGGT | 1 cut(s) | 345 |
| BshVI | ATCGAT | 1 cut(s) | 492 |
| BsiHKAI | GWGCWC | 5 cut(s) | 359, 525, 727, 733, 1655 |
| BsiSI | CCGG | 4 cut(s) | 346, 1283, 1727, 1826 |
| BslFI | GGGAC | 1 cut(s) | 243 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 1297, 1387, 1733 |
| BsmAI | GTCTC | 2 cut(s) | 197, 1805 |
| BsmBI | CGTCTC | 1 cut(s) | 197 |
| BsmFI | GGGAC | 1 cut(s) | 243 |
| BsmI | GAATGC | 1 cut(s) | 1097 |
| BsnI | GGCC | 2 cut(s) | 1589, 1889 |
| Bsp119I | TTCGAA | 1 cut(s) | 195 |
| Bsp1286I | GDGCHC | 5 cut(s) | 359, 525, 727, 733, 1655 |
| Bsp1407I | TGTACA | 1 cut(s) | 1019 |
| Bsp19I | CCATGG | 1 cut(s) | 141 |
| BspACI | CCGC | 5 cut(s) | 820, 959, 1240, 1381, 1695 |
| BspANI | GGCC | 2 cut(s) | 1589, 1889 |
| BspCNI | CTCAG | 3 cut(s) | 622, 1137, 1470 |
| BspDI | ATCGAT | 1 cut(s) | 492 |
| BspFNI | CGCG | 1 cut(s) | 322 |
| BspLI | GGNNCC | 2 cut(s) | 138, 179 |
| BspPI | GGATC | 6 cut(s) | 320, 398, 500, 545, 1553, 1789 |
| BspT104I | TTCGAA | 1 cut(s) | 195 |
| BsrDI | GCAATG | 1 cut(s) | 1866 |
| BsrFI | RCCGGY | 1 cut(s) | 345 |
| BsrGI | TGTACA | 1 cut(s) | 1019 |
| BsrI | ACTGG | 3 cut(s) | 272, 1301, 1618 |
| BssAI | RCCGGY | 1 cut(s) | 345 |
| BssECI | CCNNGG | 2 cut(s) | 141, 226 |
| BssSI | CACGAG | 1 cut(s) | 726 |
| BssT1I | CCWWGG | 1 cut(s) | 141 |
| Bst2BI | CACGAG | 1 cut(s) | 726 |
| Bst2UI | CCWGG | 1 cut(s) | 227 |
| Bst4CI | ACNGT | 7 cut(s) | 935, 956, 977, 1019, 1038, 1054, 1949 |
| Bst6I | CTCTTC | 3 cut(s) | 414, 761, 1482 |
| BstAUI | TGTACA | 1 cut(s) | 1019 |
| BstBAI | YACGTR | 2 cut(s) | 811, 1656 |
| BstBI | TTCGAA | 1 cut(s) | 195 |
| BstC8I | GCNNGC | 6 cut(s) | 324, 818, 1539, 1716, 1857, 2027 |
| BstDEI | CTNAG | 4 cut(s) | 630, 1124, 1478, 1866 |
| BstDSI | CCRYGG | 1 cut(s) | 141 |
| BstEII | GGTNACC | 1 cut(s) | 1286 |
| BstF5I | GGATG | 8 cut(s) | 499, 624, 733, 904, 967, 1236, 1623, 1981 |
| BstFNI | CGCG | 1 cut(s) | 322 |
| BstHHI | GCGC | 2 cut(s) | 322, 1207 |
| BstMAI | GTCTC | 2 cut(s) | 197, 1805 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 822, 1895 |
| BstNI | CCWGG | 1 cut(s) | 227 |
| BstNSI | RCATGY | 2 cut(s) | 1094, 1162 |
| BstPI | GGTNACC | 1 cut(s) | 1286 |
| BstSCI | CCNGG | 2 cut(s) | 225, 1282 |
| BstSNI | TACGTA | 1 cut(s) | 811 |
| BstUI | CGCG | 1 cut(s) | 322 |
| BstV1I | GCAGC | 5 cut(s) | 236, 264, 1152, 1214, 1941 |
| BstV2I | GAAGAC | 3 cut(s) | 348, 776, 1111 |
| BstX2I | RGATCY | 2 cut(s) | 505, 1545 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 1093 |
| BstYI | RGATCY | 2 cut(s) | 505, 1545 |
| Bsu15I | ATCGAT | 1 cut(s) | 492 |
| Bsu36I | CCTNAGG | 1 cut(s) | 630 |
| BsuRI | GGCC | 2 cut(s) | 1589, 1889 |
| BsuTUI | ATCGAT | 1 cut(s) | 492 |
| BtgI | CCRYGG | 1 cut(s) | 141 |
| BtrI | CACGTC | 2 cut(s) | 1045, 1852 |
| BtsCI | GGATG | 8 cut(s) | 499, 624, 733, 904, 967, 1236, 1623, 1981 |
| BtsIMutI | CAGTG | 5 cut(s) | 543, 961, 1050, 1128, 1611 |
| Cac8I | GCNNGC | 6 cut(s) | 324, 818, 1539, 1716, 1857, 2027 |
| CaiI | CAGNNNCTG | 1 cut(s) | 509 |
| CfoI | GCGC | 2 cut(s) | 322, 1207 |
| Cfr10I | RCCGGY | 1 cut(s) | 345 |
| Cfr13I | GGNCC | 2 cut(s) | 348, 1644 |
| ClaI | ATCGAT | 1 cut(s) | 492 |
| CseI | GACGC | 1 cut(s) | 845 |
| Csp6I | GTAC | 8 cut(s) | 363, 915, 936, 978, 1020, 1171, 1941, 2017 |
| CspAI | ACCGGT | 1 cut(s) | 345 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 45, 80 |
| CviQI | GTAC | 8 cut(s) | 363, 915, 936, 978, 1020, 1171, 1941, 2017 |
| DdeI | CTNAG | 4 cut(s) | 630, 1124, 1478, 1866 |
| DraIII | CACNNNGTG | 1 cut(s) | 1040 |
| EaeI | YGGCCR | 1 cut(s) | 1887 |
| Eam1104I | CTCTTC | 3 cut(s) | 414, 761, 1482 |
| EarI | CTCTTC | 3 cut(s) | 414, 761, 1482 |
| Ecl136II | GAGCTC | 1 cut(s) | 357 |
| Eco105I | TACGTA | 1 cut(s) | 811 |
| Eco130I | CCWWGG | 1 cut(s) | 141 |
| Eco24I | GRGCYC | 1 cut(s) | 359 |
| Eco47I | GGWCC | 2 cut(s) | 348, 1644 |
| Eco53kI | GAGCTC | 1 cut(s) | 357 |
| Eco72I | CACGTG | 1 cut(s) | 1656 |
| Eco81I | CCTNAGG | 1 cut(s) | 630 |
| Eco91I | GGTNACC | 1 cut(s) | 1286 |
| EcoICRI | GAGCTC | 1 cut(s) | 357 |
| EcoO65I | GGTNACC | 1 cut(s) | 1286 |
| EcoRII | CCWGG | 1 cut(s) | 225 |
| EcoT14I | CCWWGG | 1 cut(s) | 141 |
| EcoT38I | GRGCYC | 1 cut(s) | 359 |
| ErhI | CCWWGG | 1 cut(s) | 141 |
| Esp3I | CGTCTC | 1 cut(s) | 197 |
| FaqI | GGGAC | 1 cut(s) | 243 |
| FauNDI | CATATG | 1 cut(s) | 1903 |
| FbaI | TGATCA | 4 cut(s) | 130, 304, 964, 1006 |
| FblI | GTMKAC | 1 cut(s) | 834 |
| Fnu4HI | GCNGC | 6 cut(s) | 250, 278, 1141, 1203, 1696, 1930 |
| FokI | GGATG | 8 cut(s) | 486, 611, 720, 911, 974, 1223, 1630, 1988 |
| FriOI | GRGCYC | 1 cut(s) | 359 |
| Fsp4HI | GCNGC | 6 cut(s) | 250, 278, 1141, 1203, 1696, 1930 |
| FspBI | CTAG | 2 cut(s) | 233, 1542 |
| GlaI | GCGC | 2 cut(s) | 321, 1206 |
| GluI | GCNGC | 6 cut(s) | 250, 278, 1141, 1203, 1696, 1930 |
| GsuI | CTGGAG | 1 cut(s) | 183 |
| HaeIII | GGCC | 2 cut(s) | 1589, 1889 |
| HapII | CCGG | 4 cut(s) | 346, 1283, 1727, 1826 |
| HgaI | GACGC | 1 cut(s) | 845 |
| HhaI | GCGC | 2 cut(s) | 322, 1207 |
| Hin6I | GCGC | 2 cut(s) | 320, 1205 |
| HinP1I | GCGC | 2 cut(s) | 320, 1205 |
| HincII | GTYRAC | 3 cut(s) | 415, 835, 1849 |
| HindII | GTYRAC | 3 cut(s) | 415, 835, 1849 |
| HindIII | AAGCTT | 2 cut(s) | 1748, 1896 |
| HinfI | GANTC | 8 cut(s) | 31, 617, 658, 1067, 1116, 1310, 1324, 1879 |
| HpaII | CCGG | 4 cut(s) | 346, 1283, 1727, 1826 |
| HphI | GGTGA | 9 cut(s) | 376, 461, 861, 953, 1087, 1298, 1444, 1466, 1520 |
| Hpy166II | GTNNAC | 6 cut(s) | 238, 415, 835, 1050, 1773, 1849 |
| Hpy188I | TCNGA | 6 cut(s) | 36, 72, 803, 969, 1252, 1315 |
| Hpy8I | GTNNAC | 6 cut(s) | 238, 415, 835, 1050, 1773, 1849 |
| Hpy99I | CGWCG | 4 cut(s) | 692, 695, 723, 839 |
| HpyAV | CCTTC | 3 cut(s) | 536, 578, 1145 |
| HpyCH4III | ACNGT | 7 cut(s) | 935, 956, 977, 1019, 1038, 1054, 1949 |
| HpyCH4IV | ACGT | 7 cut(s) | 293, 810, 1044, 1337, 1655, 1851, 1989 |
| HpyCH4V | TGCA | 6 cut(s) | 188, 1407, 1428, 1714, 1831, 1842 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 822, 1895 |
| HpyF3I | CTNAG | 4 cut(s) | 630, 1124, 1478, 1866 |
| HpySE526I | ACGT | 7 cut(s) | 293, 810, 1044, 1337, 1655, 1851, 1989 |
| HspAI | GCGC | 2 cut(s) | 320, 1205 |
| Ksp22I | TGATCA | 4 cut(s) | 130, 304, 964, 1006 |
| LmnI | GCTCC | 3 cut(s) | 177, 362, 822 |
| Lsp1109I | GCAGC | 5 cut(s) | 236, 264, 1152, 1214, 1941 |
| LweI | GCATC | 6 cut(s) | 236, 424, 529, 610, 1245, 1657 |
| MaeI | CTAG | 2 cut(s) | 233, 1542 |
| MaeII | ACGT | 7 cut(s) | 293, 810, 1044, 1337, 1655, 1851, 1989 |
| MaeIII | GTNAC | 6 cut(s) | 382, 950, 1040, 1154, 1286, 1450 |
| MfeI | CAATTG | 1 cut(s) | 408 |
| MflI | RGATCY | 2 cut(s) | 505, 1545 |
| MhlI | GDGCHC | 5 cut(s) | 359, 525, 727, 733, 1655 |
| MlsI | TGGCCA | 1 cut(s) | 1889 |
| MluNI | TGGCCA | 1 cut(s) | 1889 |
| MlyI | GAGTC | 4 cut(s) | 1125, 1304, 1318, 1888 |
| MmeI | TCCRAC | 2 cut(s) | 820, 1640 |
| Mox20I | TGGCCA | 1 cut(s) | 1889 |
| MroXI | GAANNNNTTC | 1 cut(s) | 588 |
| MscI | TGGCCA | 1 cut(s) | 1889 |
| MslI | CAYNNNNRTG | 3 cut(s) | 561, 666, 1616 |
| Msp20I | TGGCCA | 1 cut(s) | 1889 |
| MspA1I | CMGCKG | 1 cut(s) | 1143 |
| MspI | CCGG | 4 cut(s) | 346, 1283, 1727, 1826 |
| MspR9I | CCNGG | 2 cut(s) | 227, 1284 |
| MunI | CAATTG | 1 cut(s) | 408 |
| Mva1269I | GAATGC | 1 cut(s) | 1097 |
| MvaI | CCWGG | 1 cut(s) | 227 |
| MvnI | CGCG | 1 cut(s) | 322 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 822, 1895 |
| NciI | CCSGG | 1 cut(s) | 1284 |
| NcoI | CCATGG | 1 cut(s) | 141 |
| NdeI | CATATG | 1 cut(s) | 1903 |
| NlaIV | GGNNCC | 2 cut(s) | 138, 179 |
| NmuCI | GTSAC | 5 cut(s) | 382, 1040, 1154, 1286, 1450 |
| NspI | RCATGY | 2 cut(s) | 1094, 1162 |
| NspV | TTCGAA | 1 cut(s) | 195 |
| PciI | ACATGT | 2 cut(s) | 1090, 1158 |
| PcsI | WCGNNNNNNNCGW | 5 cut(s) | 423, 724, 745, 807, 1319 |
| PctI | GAATGC | 1 cut(s) | 1097 |
| PdmI | GAANNNNTTC | 1 cut(s) | 588 |
| PfeI | GAWTC | 4 cut(s) | 31, 617, 658, 1067 |
| PflFI | GACNNNGTC | 1 cut(s) | 830 |
| PinAI | ACCGGT | 1 cut(s) | 345 |
| PkrI | GCNGC | 6 cut(s) | 251, 279, 1142, 1204, 1697, 1931 |
| PleI | GAGTC | 4 cut(s) | 1124, 1304, 1318, 1887 |
| PmaCI | CACGTG | 1 cut(s) | 1656 |
| PmlI | CACGTG | 1 cut(s) | 1656 |
| PpsI | GAGTC | 4 cut(s) | 1124, 1304, 1318, 1887 |
| Ppu21I | YACGTR | 2 cut(s) | 811, 1656 |
| PscI | ACATGT | 2 cut(s) | 1090, 1158 |
| PshBI | ATTAAT | 3 cut(s) | 18, 114, 1059 |
| Psp124BI | GAGCTC | 1 cut(s) | 359 |
| Psp6I | CCWGG | 1 cut(s) | 225 |
| PspCI | CACGTG | 1 cut(s) | 1656 |
| PspEI | GGTNACC | 1 cut(s) | 1286 |
| PspGI | CCWGG | 1 cut(s) | 225 |
| PspN4I | GGNNCC | 2 cut(s) | 138, 179 |
| PspPI | GGNCC | 2 cut(s) | 348, 1644 |
| PstNI | CAGNNNCTG | 1 cut(s) | 509 |
| PsuI | RGATCY | 2 cut(s) | 505, 1545 |
| PsyI | GACNNNGTC | 1 cut(s) | 830 |
| PvuII | CAGCTG | 1 cut(s) | 1143 |
| RsaI | GTAC | 8 cut(s) | 364, 916, 937, 979, 1021, 1172, 1942, 2018 |
| RsaNI | GTAC | 8 cut(s) | 363, 915, 936, 978, 1020, 1171, 1941, 2017 |
| RseI | CAYNNNNRTG | 3 cut(s) | 561, 666, 1616 |
| SacI | GAGCTC | 1 cut(s) | 359 |
| SalI | GTCGAC | 1 cut(s) | 833 |
| SatI | GCNGC | 6 cut(s) | 250, 278, 1141, 1203, 1696, 1930 |
| Sau96I | GGNCC | 2 cut(s) | 348, 1644 |
| ScaI | AGTACT | 1 cut(s) | 916 |
| SchI | GAGTC | 4 cut(s) | 1125, 1304, 1318, 1888 |
| ScrFI | CCNGG | 2 cut(s) | 227, 1284 |
| SduI | GDGCHC | 5 cut(s) | 359, 525, 727, 733, 1655 |
| SfaNI | GCATC | 6 cut(s) | 236, 424, 529, 610, 1245, 1657 |
| SfuI | TTCGAA | 1 cut(s) | 195 |
| SinI | GGWCC | 2 cut(s) | 348, 1644 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 561, 666, 1616 |
| SmlI | CTYRAG | 1 cut(s) | 97 |
| SmoI | CTYRAG | 1 cut(s) | 97 |
| SnaBI | TACGTA | 1 cut(s) | 811 |
| SpeI | ACTAGT | 1 cut(s) | 232 |
| SsiI | CCGC | 5 cut(s) | 820, 959, 1240, 1381, 1695 |
| SspI | AATATT | 3 cut(s) | 774, 910, 1791 |
| SspMI | CTAG | 2 cut(s) | 233, 1542 |
| SstI | GAGCTC | 1 cut(s) | 359 |
| StyD4I | CCNGG | 2 cut(s) | 225, 1282 |
| StyI | CCWWGG | 1 cut(s) | 141 |
| TaaI | ACNGT | 7 cut(s) | 935, 956, 977, 1019, 1038, 1054, 1949 |
| TaiI | ACGT | 7 cut(s) | 296, 813, 1047, 1340, 1658, 1854, 1992 |
| TaqI | TCGA | 5 cut(s) | 195, 492, 519, 834, 1569 |
| TatI | WGTACW | 3 cut(s) | 914, 1019, 2016 |
| TauI | GCSGC | 1 cut(s) | 1698 |
| TfiI | GAWTC | 4 cut(s) | 31, 617, 658, 1067 |
| TscAI | CASTG | 5 cut(s) | 550, 961, 1057, 1135, 1618 |
| TseFI | GTSAC | 5 cut(s) | 382, 1040, 1154, 1286, 1450 |
| TseI | GCWGC | 5 cut(s) | 249, 277, 1140, 1202, 1929 |
| Tsp45I | GTSAC | 5 cut(s) | 382, 1040, 1154, 1286, 1450 |
| TspGWI | ACGGA | 2 cut(s) | 770, 1245 |
| TspRI | CASTG | 5 cut(s) | 550, 961, 1057, 1135, 1618 |
| Tth111I | GACNNNGTC | 1 cut(s) | 830 |
| VpaK11BI | GGWCC | 2 cut(s) | 348, 1644 |
| VspI | ATTAAT | 3 cut(s) | 18, 114, 1059 |
| XapI | RAATTY | 3 cut(s) | 155, 1370, 1594 |
| XceI | RCATGY | 2 cut(s) | 1094, 1162 |
| XcmI | CCANNNNNNNNNTGG | 2 cut(s) | 892, 1610 |
| XmiI | GTMKAC | 1 cut(s) | 834 |
| XmnI | GAANNNNTTC | 1 cut(s) | 588 |
| XspI | CTAG | 2 cut(s) | 233, 1542 |
| ZrmI | AGTACT | 1 cut(s) | 916 |