Agglutinin domain
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 348 |
| AasI | GACNNNNNNGTC | 1 cut(s) | 512 |
| AatII | GACGTC | 1 cut(s) | 168 |
| AccI | GTMKAC | 1 cut(s) | 516 |
| AccIII | TCCGGA | 1 cut(s) | 188 |
| AciI | CCGC | 3 cut(s) | 9, 156, 415 |
| AclWI | GGATC | 3 cut(s) | 186, 199, 568 |
| AcoI | YGGCCR | 2 cut(s) | 3, 474 |
| AcsI | RAATTY | 2 cut(s) | 50, 141 |
| AcvI | CACGTG | 1 cut(s) | 87 |
| AcyI | GRCGYC | 1 cut(s) | 165 |
| AfaI | GTAC | 3 cut(s) | 160, 389, 552 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 152, 201 |
| AgsI | TTSAA | 2 cut(s) | 55, 139 |
| AjiI | CACGTC | 1 cut(s) | 340 |
| AjnI | CCWGG | 1 cut(s) | 569 |
| AleI | CACNNNNGTG | 1 cut(s) | 572 |
| AluBI | AGCT | 3 cut(s) | 135, 455, 599 |
| AluI | AGCT | 3 cut(s) | 135, 455, 599 |
| Alw26I | GTCTC | 4 cut(s) | 130, 203, 230, 416 |
| AlwI | GGATC | 3 cut(s) | 186, 199, 568 |
| Aor13HI | TCCGGA | 1 cut(s) | 188 |
| AoxI | GGCC | 3 cut(s) | 3, 93, 474 |
| ApeKI | GCWGC | 1 cut(s) | 81 |
| ApoI | RAATTY | 2 cut(s) | 50, 141 |
| AspS9I | GGNCC | 2 cut(s) | 466, 505 |
| AvaII | GGWCC | 2 cut(s) | 466, 505 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 150, 183 |
| BalI | TGGCCA | 1 cut(s) | 5 |
| BamHI | GGATCC | 1 cut(s) | 191 |
| BanII | GRGCYC | 1 cut(s) | 549 |
| BauI | CACGAG | 1 cut(s) | 103 |
| BbrPI | CACGTG | 1 cut(s) | 87 |
| BbvCI | CCTCAGC | 1 cut(s) | 31 |
| BbvI | GCAGC | 1 cut(s) | 93 |
| BccI | CCATC | 5 cut(s) | 65, 103, 283, 374, 517 |
| BciT130I | CCWGG | 1 cut(s) | 571 |
| BcoDI | GTCTC | 4 cut(s) | 130, 203, 230, 416 |
| BfmI | CTRYAG | 3 cut(s) | 252, 426, 450 |
| BisI | GCNGC | 1 cut(s) | 82 |
| BlsI | GCNGC | 1 cut(s) | 83 |
| Bme1390I | CCNGG | 1 cut(s) | 571 |
| Bme18I | GGWCC | 2 cut(s) | 466, 505 |
| BmgBI | CACGTC | 1 cut(s) | 340 |
| BmgT120I | GGNCC | 2 cut(s) | 466, 505 |
| BmiI | GGNNCC | 3 cut(s) | 193, 468, 507 |
| BmrFI | CCNGG | 1 cut(s) | 571 |
| Bpu10I | CCTNAGC | 2 cut(s) | 31, 543 |
| BpuEI | CTTGAG | 1 cut(s) | 159 |
| BsaAI | YACGTR | 1 cut(s) | 87 |
| BsaHI | GRCGYC | 1 cut(s) | 165 |
| BsaI | GGTCTC | 2 cut(s) | 203, 230 |
| BsaJI | CCNNGG | 2 cut(s) | 297, 570 |
| BsaWI | WCCGGW | 1 cut(s) | 188 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 152, 201 |
| Bse118I | RCCGGY | 1 cut(s) | 442 |
| BseAI | TCCGGA | 1 cut(s) | 188 |
| BseBI | CCWGG | 1 cut(s) | 571 |
| BseDI | CCNNGG | 2 cut(s) | 297, 570 |
| BseGI | GGATG | 4 cut(s) | 76, 114, 218, 382 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 152, 201 |
| BseMII | CTCAG | 2 cut(s) | 45, 534 |
| BseX3I | CGGCCG | 1 cut(s) | 474 |
| BseXI | GCAGC | 1 cut(s) | 93 |
| Bsh1285I | CGRYCG | 2 cut(s) | 477, 516 |
| BshFI | GGCC | 3 cut(s) | 5, 95, 476 |
| BsiEI | CGRYCG | 2 cut(s) | 477, 516 |
| BsiSI | CCGG | 3 cut(s) | 189, 443, 503 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 152, 201 |
| BsmAI | GTCTC | 4 cut(s) | 130, 203, 230, 416 |
| BsnI | GGCC | 3 cut(s) | 5, 95, 476 |
| Bso31I | GGTCTC | 2 cut(s) | 203, 230 |
| Bsp1286I | GDGCHC | 1 cut(s) | 549 |
| Bsp13I | TCCGGA | 1 cut(s) | 188 |
| Bsp1407I | TGTACA | 1 cut(s) | 387 |
| Bsp143I | GATC | 2 cut(s) | 191, 560 |
| BspACI | CCGC | 3 cut(s) | 9, 156, 415 |
| BspANI | GGCC | 3 cut(s) | 5, 95, 476 |
| BspCNI | CTCAG | 2 cut(s) | 44, 535 |
| BspEI | TCCGGA | 1 cut(s) | 188 |
| BspLI | GGNNCC | 3 cut(s) | 193, 468, 507 |
| BspPI | GGATC | 3 cut(s) | 186, 199, 568 |
| BspTNI | GGTCTC | 2 cut(s) | 203, 230 |
| BsrFI | RCCGGY | 1 cut(s) | 442 |
| BsrGI | TGTACA | 1 cut(s) | 387 |
| BssAI | RCCGGY | 1 cut(s) | 442 |
| BssECI | CCNNGG | 2 cut(s) | 297, 570 |
| BssMI | GATC | 2 cut(s) | 191, 560 |
| BssNI | GRCGYC | 1 cut(s) | 165 |
| BssSI | CACGAG | 1 cut(s) | 103 |
| BssT1I | CCWWGG | 1 cut(s) | 297 |
| Bst2BI | CACGAG | 1 cut(s) | 103 |
| Bst2UI | CCWGG | 1 cut(s) | 571 |
| Bst4CI | ACNGT | 3 cut(s) | 46, 256, 555 |
| BstACI | GRCGYC | 1 cut(s) | 165 |
| BstAUI | TGTACA | 1 cut(s) | 387 |
| BstBAI | YACGTR | 1 cut(s) | 87 |
| BstC8I | GCNNGC | 3 cut(s) | 19, 97, 133 |
| BstDEI | CTNAG | 2 cut(s) | 31, 543 |
| BstF5I | GGATG | 4 cut(s) | 76, 114, 218, 382 |
| BstKTI | GATC | 2 cut(s) | 194, 563 |
| BstMAI | GTCTC | 4 cut(s) | 130, 203, 230, 416 |
| BstMBI | GATC | 2 cut(s) | 191, 560 |
| BstMCI | CGRYCG | 2 cut(s) | 477, 516 |
| BstNI | CCWGG | 1 cut(s) | 571 |
| BstNSI | RCATGY | 1 cut(s) | 316 |
| BstSCI | CCNGG | 1 cut(s) | 569 |
| BstSFI | CTRYAG | 3 cut(s) | 252, 426, 450 |
| BstV1I | GCAGC | 1 cut(s) | 93 |
| BstX2I | RGATCY | 1 cut(s) | 191 |
| BstYI | RGATCY | 1 cut(s) | 191 |
| BstZI | CGGCCG | 1 cut(s) | 474 |
| BsuRI | GGCC | 3 cut(s) | 5, 95, 476 |
| BtrI | CACGTC | 1 cut(s) | 340 |
| BtsCI | GGATG | 4 cut(s) | 76, 114, 218, 382 |
| BtsI | GCAGTG | 1 cut(s) | 168 |
| BtsIMutI | CAGTG | 3 cut(s) | 42, 168, 261 |
| Cac8I | GCNNGC | 3 cut(s) | 19, 97, 133 |
| Cfr10I | RCCGGY | 1 cut(s) | 442 |
| Cfr13I | GGNCC | 2 cut(s) | 466, 505 |
| Csp6I | GTAC | 3 cut(s) | 159, 388, 551 |
| CviAII | CATG | 1 cut(s) | 313 |
| CviQI | GTAC | 3 cut(s) | 159, 388, 551 |
| DdeI | CTNAG | 2 cut(s) | 31, 543 |
| DpnI | GATC | 2 cut(s) | 193, 562 |
| DpnII | GATC | 2 cut(s) | 191, 560 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 512 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 512 |
| EaeI | YGGCCR | 2 cut(s) | 3, 474 |
| EagI | CGGCCG | 1 cut(s) | 474 |
| EclXI | CGGCCG | 1 cut(s) | 474 |
| Eco130I | CCWWGG | 1 cut(s) | 297 |
| Eco24I | GRGCYC | 1 cut(s) | 549 |
| Eco31I | GGTCTC | 2 cut(s) | 203, 230 |
| Eco47I | GGWCC | 2 cut(s) | 466, 505 |
| Eco52I | CGGCCG | 1 cut(s) | 474 |
| Eco72I | CACGTG | 1 cut(s) | 87 |
| EcoRI | GAATTC | 1 cut(s) | 50 |
| EcoRII | CCWGG | 1 cut(s) | 569 |
| EcoT14I | CCWWGG | 1 cut(s) | 297 |
| EcoT38I | GRGCYC | 1 cut(s) | 549 |
| ErhI | CCWWGG | 1 cut(s) | 297 |
| FaeI | CATG | 1 cut(s) | 316 |
| FaiI | YATR | 9 cut(s) | 64, 198, 275, 314, 348, 369, 406, 452, 596 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 327, 359 |
| FatI | CATG | 1 cut(s) | 312 |
| FblI | GTMKAC | 1 cut(s) | 516 |
| Fnu4HI | GCNGC | 1 cut(s) | 82 |
| FokI | GGATG | 4 cut(s) | 83, 121, 205, 389 |
| FriOI | GRGCYC | 1 cut(s) | 549 |
| Fsp4HI | GCNGC | 1 cut(s) | 82 |
| GluI | GCNGC | 1 cut(s) | 82 |
| HaeIII | GGCC | 3 cut(s) | 5, 95, 476 |
| HapII | CCGG | 3 cut(s) | 189, 443, 503 |
| Hin1I | GRCGYC | 1 cut(s) | 165 |
| Hin1II | CATG | 1 cut(s) | 316 |
| HincII | GTYRAC | 1 cut(s) | 517 |
| HindII | GTYRAC | 1 cut(s) | 517 |
| HpaII | CCGG | 3 cut(s) | 189, 443, 503 |
| Hpy166II | GTNNAC | 3 cut(s) | 388, 390, 517 |
| Hpy188I | TCNGA | 2 cut(s) | 223, 333 |
| Hpy188III | TCNNGA | 2 cut(s) | 189, 558 |
| Hpy8I | GTNNAC | 3 cut(s) | 388, 390, 517 |
| Hpy99I | CGWCG | 1 cut(s) | 475 |
| HpyAV | CCTTC | 2 cut(s) | 85, 367 |
| HpyCH4III | ACNGT | 3 cut(s) | 46, 256, 555 |
| HpyCH4IV | ACGT | 3 cut(s) | 86, 165, 339 |
| HpyCH4V | TGCA | 2 cut(s) | 62, 577 |
| HpyF3I | CTNAG | 2 cut(s) | 31, 543 |
| HpySE526I | ACGT | 3 cut(s) | 86, 165, 339 |
| Hsp92I | GRCGYC | 1 cut(s) | 165 |
| Hsp92II | CATG | 1 cut(s) | 316 |
| Kpn2I | TCCGGA | 1 cut(s) | 188 |
| Kzo9I | GATC | 2 cut(s) | 191, 560 |
| Lsp1109I | GCAGC | 1 cut(s) | 93 |
| MaeII | ACGT | 3 cut(s) | 86, 165, 339 |
| MaeIII | GTNAC | 2 cut(s) | 166, 308 |
| MalI | GATC | 2 cut(s) | 193, 562 |
| MboI | GATC | 2 cut(s) | 191, 560 |
| MflI | RGATCY | 1 cut(s) | 191 |
| MhlI | GDGCHC | 1 cut(s) | 549 |
| MlsI | TGGCCA | 1 cut(s) | 5 |
| MluCI | AATT | 5 cut(s) | 50, 141, 400, 409, 457 |
| MluNI | TGGCCA | 1 cut(s) | 5 |
| MnlI | CCTC | 1 cut(s) | 40 |
| Mox20I | TGGCCA | 1 cut(s) | 5 |
| MroI | TCCGGA | 1 cut(s) | 188 |
| MscI | TGGCCA | 1 cut(s) | 5 |
| MslI | CAYNNNNRTG | 1 cut(s) | 572 |
| Msp20I | TGGCCA | 1 cut(s) | 5 |
| MspI | CCGG | 3 cut(s) | 189, 443, 503 |
| MspR9I | CCNGG | 1 cut(s) | 571 |
| MvaI | CCWGG | 1 cut(s) | 571 |
| NdeII | GATC | 2 cut(s) | 191, 560 |
| NlaIII | CATG | 1 cut(s) | 316 |
| NlaIV | GGNNCC | 3 cut(s) | 193, 468, 507 |
| NmuCI | GTSAC | 2 cut(s) | 166, 308 |
| NspI | RCATGY | 1 cut(s) | 316 |
| OliI | CACNNNNGTG | 1 cut(s) | 572 |
| PkrI | GCNGC | 1 cut(s) | 83 |
| PmaCI | CACGTG | 1 cut(s) | 87 |
| PmlI | CACGTG | 1 cut(s) | 87 |
| Ppu21I | YACGTR | 1 cut(s) | 87 |
| PsiI | TTATAA | 1 cut(s) | 348 |
| Psp6I | CCWGG | 1 cut(s) | 569 |
| PspCI | CACGTG | 1 cut(s) | 87 |
| PspGI | CCWGG | 1 cut(s) | 569 |
| PspN4I | GGNNCC | 3 cut(s) | 193, 468, 507 |
| PspPI | GGNCC | 2 cut(s) | 466, 505 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 522, 554 |
| PsuI | RGATCY | 1 cut(s) | 191 |
| RsaI | GTAC | 3 cut(s) | 160, 389, 552 |
| RsaNI | GTAC | 3 cut(s) | 159, 388, 551 |
| RseI | CAYNNNNRTG | 1 cut(s) | 572 |
| SalI | GTCGAC | 1 cut(s) | 515 |
| SatI | GCNGC | 1 cut(s) | 82 |
| Sau3AI | GATC | 2 cut(s) | 191, 560 |
| Sau96I | GGNCC | 2 cut(s) | 466, 505 |
| ScrFI | CCNGG | 1 cut(s) | 571 |
| SduI | GDGCHC | 1 cut(s) | 549 |
| SfcI | CTRYAG | 3 cut(s) | 252, 426, 450 |
| SinI | GGWCC | 2 cut(s) | 466, 505 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 572 |
| SmlI | CTYRAG | 1 cut(s) | 174 |
| SmoI | CTYRAG | 1 cut(s) | 174 |
| Sse9I | AATT | 5 cut(s) | 50, 141, 400, 409, 457 |
| SsiI | CCGC | 3 cut(s) | 9, 156, 415 |
| StyD4I | CCNGG | 1 cut(s) | 569 |
| StyI | CCWWGG | 1 cut(s) | 297 |
| TaaI | ACNGT | 3 cut(s) | 46, 256, 555 |
| TaiI | ACGT | 3 cut(s) | 89, 168, 342 |
| TaqI | TCGA | 1 cut(s) | 516 |
| TaqII | GACCGA | 1 cut(s) | 502 |
| TasI | AATT | 5 cut(s) | 50, 141, 400, 409, 457 |
| TatI | WGTACW | 1 cut(s) | 387 |
| TscAI | CASTG | 3 cut(s) | 49, 175, 261 |
| TseFI | GTSAC | 2 cut(s) | 166, 308 |
| TseI | GCWGC | 1 cut(s) | 81 |
| Tsp45I | GTSAC | 2 cut(s) | 166, 308 |
| TspDTI | ATGAA | 2 cut(s) | 139, 393 |
| TspGWI | ACGGA | 2 cut(s) | 349, 448 |
| TspRI | CASTG | 3 cut(s) | 49, 175, 261 |
| VpaK11BI | GGWCC | 2 cut(s) | 466, 505 |
| XapI | RAATTY | 2 cut(s) | 50, 141 |
| XceI | RCATGY | 1 cut(s) | 316 |
| XmiI | GTMKAC | 1 cut(s) | 516 |
| ZraI | GACGTC | 1 cut(s) | 166 |