MD01G1104300.v1.1

Agglutinin domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0000217
Physical Location & Seq
Forward (+)
21733076 .. 21734572
1497 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1104300.v1.1.491

Sequence Viewer

Length: 1497 bp
ATGTCAACATTGCCCAGGTTTGTGGTGATCAAGTCGAACTACAATGACAAATATTTGCGATTCAATATTGATGCAGATGAGAATCTGAAGCGAGTCATGCCAGCTGCAAGGTATATCAATTTCACAGGAGAGGAAGCATGGAGCGAATACACAAAATTTGAAGTGGAGAAGGTAAAGAGTAATGGGAATGAGGGACTAGTGCACATTAGATGCTGCTACAACAACAAATACTGGGTGATGCCTGCTTGTGGTTATACTCAGATTGTTGCTGGTGCTGATGAACCAGACGAAGACAAATCCAAGTTCTCGTGTACCTTGTTTGAGCCTATCTACGCATATGATGATAGTCTCCCGGTTCATGATGCCAGAAGTACTAATTATATAGTACTCCGATTCCGCCATGTCCACAATGGAAAGTACGTAGCATTGGTACAAAACAATCCGAATGACAAATACTGGGGTTTCATATACACGTATGGGGAATCGCCTAGCCAGAAGTTTATGGATGTGTTCATGGTCATCGACTGGGAATCATTGGTGCCAGTCAAGTTACTTCCCAAGCATGTGGCGTTCAAAGGCCGTAATGGCAACTACCTCAGTGCCCGCTTGATCGACCAACAACCACATCTGCTGCAGTTCGAATCCATCGACATTGAAGATCCATCTGTGGGAAACGAGGTCTTCACCAACGCTGATGGAACTGTCCGCATAAAGTCCAATTCCTCGGGGAAATTCTGGAGACTTTCCAAGAACGACTGGATATGTGCCGACTCAGATGACTCCAGCGACAATGATTCCGACACCTTGTTTTGGCCGATCAAAGTAGGTGACAATGTCATTGCTCTCCGCAGCATACGCAACAATAATTTCTGCAAGAGATTTACCATCAGCACAATTACTAGCAGTCTCAATGCAAATGCCCCCACTATTGACAAAGAGGCGTACCTTGAGGTGGAAGAGGTTCTCCCCTTATGGAGGGATATCAGTGGTATTTCTCTTCGCCTCTATCGTGCAAGGATCTACAACCAACACGTTGTTGAAATGGCCGTGGGAGAGGTCGTTAACCGTACCAAAGAATCTTACCCTGTAGAGGTAAAGCTTTCGTATACACAAACAACGAGAAGTACTTGGAAGTACTCTAGCGTCTTGTCGATTTTGAAGTTGCCTGGTGTCAAGAGCAGTACCAATACCGCAATTCCAATAATTGCAGACGATAACAAGGTGAAGATTGGATTTACTGGAACAGCTCAATTTGGAGAAACTGAGACAGTCACAAGTAGTCAAAAGGAGGTTGTGTATAAAGATGTTCTACAAGCAATGACTAGGGTGAAGGTGAAGTATGTAGCAACCGAGGCTTCATTTGATGTTCCCTTCTCCTACACACAGCGCGACACTCTCATGAGCGGGGAAACAATTACTAGCAACATAGACGATGGTGTTTATACTGGTGTCAATACCTTCGACTTCAAGTTCGACACCACAGAAGAAAAGCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aerolysin Agglutinin ETX_MTX2

Protein Analysis

499

Amino Acids

56.75

Weight (kDa)

5.58

Isoelectric Point (pI)

33.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Agglutinin PF07468 3 - 114 9.7e-15 Agglutinin domain
Agglutinin PF07468 188 - 315 4.4e-19 Agglutinin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000217)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g17710 FvH4_6g45590 FvH4_6g45591 FvH4_6g45770 FvH4_6g45770 FvH4_6g46030 FvH4_6g46040 FvH4_7g07020 FvH4_7g07071 FvH4_7g07080
malus_domestica MD01G1104300.v1.1 MD02G1240000.v1.1 MD02G1240200.v1.1 MD03G1201400.v1.1 MD03G1201500.v1.1 MD07G1171100.v1.1
prunus_persica Prupe.2G087400_v2.0.a1 Prupe.2G097500_v2.0.a1 Prupe.2G097700_v2.0.a1 Prupe.2G097800_v2.0.a1 Prupe.3G153100_v2.0.a1 Prupe.3G153300_v2.0.a1 Prupe.3G153600_v2.0.a1 Prupe.5G237200_v2.0.a1
pyrus_communis pycom03g15270 pycom06g20700 pycom07g06030 pycom10g01810 pycom10g01820 pycom10g12090 pycom14g20090 pycom14g20100 pycom14g20110 pycom14g20120
rosa_chinensis RchiOBHm_Chr1g0336971 RchiOBHm_Chr1g0337031 RchiOBHm_Chr2g0163571 RchiOBHm_Chr2g0163591 RchiOBHm_Chr2g0163631 RchiOBHm_Chr2g0163661 RchiOBHm_Chr2g0163671 RchiOBHm_Chr2g0163691 RchiOBHm_Chr2g0163741 RchiOBHm_Chr5g0011821 RchiOBHm_Chr5g0011831 RchiOBHm_Chr5g0011841 RchiOBHm_Chr5g0053101 RchiOBHm_Chr5g0072501
rosa_laevigata RLG00000010009 RLG00000021413 RLG00000029310 RLG00000029318 RLG00000036313
rosa_multiflora Rmu_co7975848.1_g000001 Rmu_co8020886.1_g000001 Rmu_co8136580.1_g000001 Rmu_co8177372.1_g000001 Rmu_co8207014.1_g000001 Rmu_co8232187.1_g000001 Rmu_co8277595.1_g000001 Rmu_co8389219.1_g000001 Rmu_co8427877.1_g000001 Rmu_co8467587.1_g000001 Rmu_sc0001475.1_g000015 Rmu_sc0001475.1_g000016 Rmu_sc0001475.1_g000017 Rmu_sc0003462.1_g000002 Rmu_sc0003462.1_g000003 Rmu_sc0003484.1_g000002 Rmu_sc0004918.1_g000004 Rmu_sc0004918.1_g000006 Rmu_sc0004918.1_g000007 Rmu_sc0004918.1_g000009 Rmu_sc0006596.1_g000003 Rmu_sc0006596.1_g000005 Rmu_sc0006596.1_g000010 Rmu_sc0008676.1_g000021 Rmu_sc0014330.1_g000002 Rmu_sc0016133.1_g000002 Rmu_sc0016133.1_g000003 Rmu_sc0016133.1_g000004 Rmu_sc0026453.1_g000003 Rmu_sc0026453.1_g000004 Rmu_sc0030660.1_g000001
rosa_roxburghii Rroxscaffold_1G00008700 Rroxscaffold_1G00064740 Rroxscaffold_2G00086950 Rroxscaffold_2G00087100 Rroxscaffold_2G00087140 Rroxscaffold_4G00302080 Rroxscaffold_4G00315020 Rroxscaffold_4G00315170 Rroxscaffold_5G00358800
rosa_rugosa Rorug01G0129200 Rorug01G0130600 Rorug01G0130700 Rorug02G0503700 Rorug02G0503800 Rorug02G0503900 Rorug02G0504000 Rorug02G0504300 Rorug05G0268200 Rorug05G0418700 Rorug05G0418800
rosa_samantha Rh1AG151100 Rh1AG151300 Rh1BG118400 Rh1BG120600 Rh1CG141500 Rh1CG142500 Rh1DG157300 Rh1DG158500 Rh2AG570600 Rh2AG570800 Rh2AG571200 Rh2AG571300 Rh2AG571500 Rh2AG571900 Rh2AG572000 Rh2AG572200 Rh2CG552600 Rh2CG552800 Rh2CG553000 Rh2CG553300 Rh2CG553400 Rh2CG553700 Rh5CG100400 Rh5CG100500 Rh5CG100600 Rh5CG100700 Rh5CG519100 Rh5DG086600 Rh5DG086700 Rh5DG086800 Rh5DG086900 Rh5DG087000 Rh5DG087100 Rh5DG367600 Rh5DG507300
rosa_wichuraiana Rw1G012130 Rw1G012240 Rw1G012380 Rw2G047240 Rw2G047260 Rw2G047270 Rw2G047290 Rw5G007940 Rw5G044120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 538
AccBSI CCGCTC 1 cut(s) 1404
AccI GTMKAC 1 cut(s) 1106
AccII CGCG 1 cut(s) 1389
AciI CCGC 6 cut(s) 397, 604, 706, 847, 1191, 1404
AclWI GGATC 2 cut(s) 653, 1025
AcoI YGGCCR 2 cut(s) 812, 1044
AcsI RAATTY 2 cut(s) 155, 731
AcuI CTGAAG 1 cut(s) 107
AfiI CCNNNNNNNGG 6 cut(s) 248, 668, 810, 952, 975, 1090
AflIII ACRYGT 2 cut(s) 471, 1030
AgsI TTSAA 7 cut(s) 64, 161, 574, 656, 1040, 1159, 1468
AhlI ACTAGT 1 cut(s) 196
AjnI CCWGG 2 cut(s) 14, 1165
AluBI AGCT 4 cut(s) 104, 1099, 1247, 1492
AluI AGCT 4 cut(s) 104, 1099, 1247, 1492
Alw21I GWGCWC 1 cut(s) 204
Alw26I GTCTC 4 cut(s) 353, 733, 911, 1259
Alw44I GTGCAC 1 cut(s) 200
AlwI GGATC 2 cut(s) 653, 1025
Ama87I CYCGRG 1 cut(s) 724
AoxI GGCC 3 cut(s) 577, 812, 1044
ApaLI GTGCAC 1 cut(s) 200
ApeKI GCWGC 4 cut(s) 104, 213, 631, 849
ApoI RAATTY 2 cut(s) 155, 731
ArsI GACNNNNNNTTYG 2 cut(s) 1454, 1486
Asp700I GAANNNNTTC 1 cut(s) 960
AspLEI GCGC 1 cut(s) 1389
AsuC2I CCSGG 1 cut(s) 353
AsuHPI GGTGA 7 cut(s) 37, 247, 676, 839, 1234, 1339, 1345
AsuII TTCGAA 1 cut(s) 639
AvaI CYCGRG 1 cut(s) 724
BaeGI GKGCMC 2 cut(s) 204, 604
BanI GGYRCC 1 cut(s) 538
BauI CACGAG 1 cut(s) 307
BbsI GAAGAC 2 cut(s) 297, 673
Bbv12I GWGCWC 1 cut(s) 204
BbvI GCAGC 4 cut(s) 91, 200, 618, 861
BccI CCATC 5 cut(s) 653, 670, 689, 893, 1427
BceAI ACGGC 2 cut(s) 564, 1031
BciT130I CCWGG 2 cut(s) 16, 1167
BclI TGATCA 1 cut(s) 27
BcnI CCSGG 1 cut(s) 353
BcoDI GTCTC 4 cut(s) 353, 733, 911, 1259
BcuI ACTAGT 1 cut(s) 196
BfaI CTAG 6 cut(s) 197, 489, 900, 1140, 1323, 1419
BfmI CTRYAG 2 cut(s) 632, 1086
BglI GCCNNNNNGGC 1 cut(s) 585
BisI GCNGC 4 cut(s) 105, 214, 632, 850
BlsI GCNGC 4 cut(s) 106, 215, 633, 851
BmcAI AGTACT 4 cut(s) 373, 387, 1126, 1136
Bme1390I CCNGG 3 cut(s) 16, 353, 1167
BmeT110I CYCGRG 1 cut(s) 724
BmiI GGNNCC 1 cut(s) 540
BmrFI CCNGG 3 cut(s) 16, 353, 1167
BmrI ACTGGG 3 cut(s) 241, 466, 535
BmsI GCATC 4 cut(s) 61, 200, 228, 352
BmuI ACTGGG 3 cut(s) 241, 466, 535
BpiI GAAGAC 2 cut(s) 297, 673
BpmI CTGGAG 2 cut(s) 757, 766
Bpu14I TTCGAA 1 cut(s) 639
BpuEI CTTGAG 1 cut(s) 968
BpuMI CCSGG 1 cut(s) 353
BsaAI YACGTR 2 cut(s) 421, 474
BsaBI GATNNNNATC 1 cut(s) 81
BsaJI CCNNGG 4 cut(s) 14, 723, 1047, 1350
BsaXI ACNNNNNCTCC 4 cut(s) 1044, 1074, 1280, 1310
Bsc4I CCNNNNNNNGG 6 cut(s) 248, 668, 810, 952, 975, 1090
Bse1I ACTGG 7 cut(s) 236, 461, 530, 542, 761, 1243, 1450
Bse3DI GCAATG 3 cut(s) 8, 837, 1323
Bse8I GATNNNNATC 1 cut(s) 81
BseBI CCWGG 2 cut(s) 16, 1167
BseDI CCNNGG 4 cut(s) 14, 723, 1047, 1350
BseGI GGATG 1 cut(s) 511
BseJI GATNNNNATC 1 cut(s) 81
BseLI CCNNNNNNNGG 6 cut(s) 248, 668, 810, 952, 975, 1090
BseMI GCAATG 3 cut(s) 8, 837, 1323
BseMII CTCAG 4 cut(s) 272, 610, 786, 1254
BseNI ACTGG 7 cut(s) 236, 461, 530, 542, 761, 1243, 1450
BseSI GKGCMC 2 cut(s) 204, 604
BseXI GCAGC 4 cut(s) 91, 200, 618, 861
Bsh1236I CGCG 1 cut(s) 1389
BshFI GGCC 3 cut(s) 579, 814, 1046
BshNI GGYRCC 1 cut(s) 538
BsiHKAI GWGCWC 1 cut(s) 204
BsiHKCI CYCGRG 1 cut(s) 724
BsiSI CCGG 1 cut(s) 353
BslFI GGGAC 1 cut(s) 207
BslI CCNNNNNNNGG 6 cut(s) 248, 668, 810, 952, 975, 1090
BsmAI GTCTC 4 cut(s) 353, 733, 911, 1259
BsmFI GGGAC 1 cut(s) 207
BsnI GGCC 3 cut(s) 579, 814, 1046
BsoBI CYCGRG 1 cut(s) 724
Bsp119I TTCGAA 1 cut(s) 639
Bsp1286I GDGCHC 2 cut(s) 204, 604
Bsp143I GATC 5 cut(s) 27, 609, 658, 816, 1017
BspACI CCGC 6 cut(s) 397, 604, 706, 847, 1191, 1404
BspANI GGCC 3 cut(s) 579, 814, 1046
BspCNI CTCAG 4 cut(s) 271, 609, 785, 1255
BspFNI CGCG 1 cut(s) 1389
BspHI TCATGA 2 cut(s) 358, 1398
BspLI GGNNCC 1 cut(s) 540
BspMAI CTGCAG 1 cut(s) 636
BspPI GGATC 2 cut(s) 653, 1025
BspT104I TTCGAA 1 cut(s) 639
BspT107I GGYRCC 1 cut(s) 538
BsrBI CCGCTC 1 cut(s) 1404
BsrDI GCAATG 3 cut(s) 8, 837, 1323
BsrI ACTGG 7 cut(s) 236, 461, 530, 542, 761, 1243, 1450
BssECI CCNNGG 4 cut(s) 14, 723, 1047, 1350
BssMI GATC 5 cut(s) 27, 609, 658, 816, 1017
BssNAI GTATAC 1 cut(s) 1107
BssSI CACGAG 1 cut(s) 307
Bst1107I GTATAC 1 cut(s) 1107
Bst2BI CACGAG 1 cut(s) 307
Bst2UI CCWGG 2 cut(s) 16, 1167
Bst4CI ACNGT 3 cut(s) 703, 1067, 1270
Bst6I CTCTTC 2 cut(s) 951, 1002
BstBAI YACGTR 2 cut(s) 421, 474
BstBI TTCGAA 1 cut(s) 639
BstC8I GCNNGC 3 cut(s) 102, 243, 604
BstDEI CTNAG 4 cut(s) 258, 596, 772, 1263
BstDSI CCRYGG 1 cut(s) 1047
BstENI CCTNNNNNAGG 1 cut(s) 973
BstF5I GGATG 1 cut(s) 511
BstFNI CGCG 1 cut(s) 1389
BstHHI GCGC 1 cut(s) 1389
BstKTI GATC 5 cut(s) 30, 612, 661, 819, 1020
BstMAI GTCTC 4 cut(s) 353, 733, 911, 1259
BstMBI GATC 5 cut(s) 27, 609, 658, 816, 1017
BstMWI GCNNNNNNNGC 4 cut(s) 97, 585, 855, 1352
BstNI CCWGG 2 cut(s) 16, 1167
BstNSI RCATGY 1 cut(s) 566
BstSCI CCNGG 3 cut(s) 14, 351, 1165
BstSFI CTRYAG 2 cut(s) 632, 1086
BstSLI GKGCMC 2 cut(s) 204, 604
BstSNI TACGTA 1 cut(s) 421
BstUI CGCG 1 cut(s) 1389
BstV1I GCAGC 4 cut(s) 91, 200, 618, 861
BstV2I GAAGAC 2 cut(s) 297, 673
BstX2I RGATCY 2 cut(s) 658, 1017
BstXI CCANNNNNNTGG 2 cut(s) 22, 565
BstYI RGATCY 2 cut(s) 658, 1017
BstZ17I GTATAC 1 cut(s) 1107
BsuRI GGCC 3 cut(s) 579, 814, 1046
BtgI CCRYGG 1 cut(s) 1047
BtsCI GGATG 1 cut(s) 511
BtsIMutI CAGTG 2 cut(s) 604, 991
Cac8I GCNNGC 3 cut(s) 102, 243, 604
CciI TCATGA 2 cut(s) 358, 1398
CfoI GCGC 1 cut(s) 1389
CseI GACGC 1 cut(s) 1132
CviAII CATG 7 cut(s) 97, 138, 359, 401, 514, 563, 1399
DdeI CTNAG 4 cut(s) 258, 596, 772, 1263
DpnI GATC 5 cut(s) 29, 611, 660, 818, 1019
DpnII GATC 5 cut(s) 27, 609, 658, 816, 1017
EaeI YGGCCR 2 cut(s) 812, 1044
Eam1104I CTCTTC 2 cut(s) 951, 1002
EarI CTCTTC 2 cut(s) 951, 1002
EciI GGCGGA 1 cut(s) 386
Eco105I TACGTA 1 cut(s) 421
Eco32I GATATC 1 cut(s) 982
Eco57I CTGAAG 1 cut(s) 107
Eco88I CYCGRG 1 cut(s) 724
EcoNI CCTNNNNNAGG 1 cut(s) 973
EcoRII CCWGG 2 cut(s) 14, 1165
EcoRV GATATC 1 cut(s) 982
FaeI CATG 7 cut(s) 100, 141, 362, 404, 517, 566, 1402
FalI AAGNNNNNCTT 1 cut(s) 1476
FaqI GGGAC 1 cut(s) 207
FatI CATG 7 cut(s) 96, 137, 358, 400, 513, 562, 1398
FauI CCCGC 2 cut(s) 611, 1397
FauNDI CATATG 1 cut(s) 337
FbaI TGATCA 1 cut(s) 27
FblI GTMKAC 1 cut(s) 1106
Fnu4HI GCNGC 4 cut(s) 105, 214, 632, 850
FokI GGATG 1 cut(s) 518
Fsp4HI GCNGC 4 cut(s) 105, 214, 632, 850
FspBI CTAG 6 cut(s) 197, 489, 900, 1140, 1323, 1419
GlaI GCGC 1 cut(s) 1388
GluI GCNGC 4 cut(s) 105, 214, 632, 850
GsuI CTGGAG 2 cut(s) 757, 766
HaeIII GGCC 3 cut(s) 579, 814, 1046
HapII CCGG 1 cut(s) 353
HgaI GACGC 1 cut(s) 1132
HhaI GCGC 1 cut(s) 1389
Hin1II CATG 7 cut(s) 100, 141, 362, 404, 517, 566, 1402
Hin6I GCGC 1 cut(s) 1387
HinP1I GCGC 1 cut(s) 1387
HincII GTYRAC 2 cut(s) 6, 1063
HindII GTYRAC 2 cut(s) 6, 1063
HindIII AAGCTT 2 cut(s) 1097, 1490
HpaI GTTAAC 1 cut(s) 1063
HpaII CCGG 1 cut(s) 353
HphI GGTGA 7 cut(s) 37, 247, 676, 839, 1234, 1339, 1345
Hpy166II GTNNAC 6 cut(s) 6, 202, 312, 406, 1063, 1107
Hpy188I TCNGA 6 cut(s) 87, 261, 392, 444, 775, 799
Hpy188III TCNNGA 4 cut(s) 359, 736, 1174, 1399
Hpy8I GTNNAC 6 cut(s) 6, 202, 312, 406, 1063, 1107
HpyAV CCTTC 4 cut(s) 163, 1324, 1381, 1468
HpyCH4III ACNGT 3 cut(s) 703, 1067, 1270
HpyCH4IV ACGT 3 cut(s) 420, 473, 1032
HpyCH4V TGCA 8 cut(s) 74, 107, 202, 634, 873, 914, 1013, 1208
HpyF10VI GCNNNNNNNGC 4 cut(s) 97, 585, 855, 1352
HpyF3I CTNAG 4 cut(s) 258, 596, 772, 1263
HpySE526I ACGT 3 cut(s) 420, 473, 1032
Hsp92II CATG 7 cut(s) 100, 141, 362, 404, 517, 566, 1402
HspAI GCGC 1 cut(s) 1387
Ksp22I TGATCA 1 cut(s) 27
KspAI GTTAAC 1 cut(s) 1063
Kzo9I GATC 5 cut(s) 27, 609, 658, 816, 1017
LmnI GCTCC 1 cut(s) 141
Lsp1109I GCAGC 4 cut(s) 91, 200, 618, 861
LweI GCATC 4 cut(s) 61, 200, 228, 352
MaeI CTAG 6 cut(s) 197, 489, 900, 1140, 1323, 1419
MaeII ACGT 3 cut(s) 420, 473, 1032
MaeIII GTNAC 3 cut(s) 549, 827, 1270
MalI GATC 5 cut(s) 29, 611, 660, 818, 1019
MbiI CCGCTC 1 cut(s) 1404
MboI GATC 5 cut(s) 27, 609, 658, 816, 1017
MboII GAAGA 7 cut(s) 302, 668, 673, 968, 989, 1237, 1496
MflI RGATCY 2 cut(s) 658, 1017
MhlI GDGCHC 2 cut(s) 204, 604
MlyI GAGTC 3 cut(s) 102, 764, 773
MmeI TCCRAC 1 cut(s) 822
MroXI GAANNNNTTC 1 cut(s) 960
MseI TTAA 1 cut(s) 1062
MslI CAYNNNNRTG 1 cut(s) 1397
MspA1I CMGCKG 1 cut(s) 104
MspI CCGG 1 cut(s) 353
MspR9I CCNGG 3 cut(s) 16, 353, 1167
MvaI CCWGG 2 cut(s) 16, 1167
MvnI CGCG 1 cut(s) 1389
MwoI GCNNNNNNNGC 4 cut(s) 97, 585, 855, 1352
NciI CCSGG 1 cut(s) 353
NdeI CATATG 1 cut(s) 337
NdeII GATC 5 cut(s) 27, 609, 658, 816, 1017
NlaIII CATG 7 cut(s) 100, 141, 362, 404, 517, 566, 1402
NlaIV GGNNCC 1 cut(s) 540
NmuCI GTSAC 2 cut(s) 827, 1270
NspI RCATGY 1 cut(s) 566
NspV TTCGAA 1 cut(s) 639
PagI TCATGA 2 cut(s) 358, 1398
PcsI WCGNNNNNNNCGW 2 cut(s) 645, 1006
PdmI GAANNNNTTC 1 cut(s) 960
PfeI GAWTC 8 cut(s) 60, 82, 393, 482, 530, 641, 794, 1076
PflFI GACNNNGTC 1 cut(s) 833
PkrI GCNGC 4 cut(s) 106, 215, 633, 851
PleI GAGTC 3 cut(s) 101, 764, 773
PpsI GAGTC 3 cut(s) 101, 764, 773
Ppu21I YACGTR 2 cut(s) 421, 474
Psp6I CCWGG 2 cut(s) 14, 1165
PspGI CCWGG 2 cut(s) 14, 1165
PspN4I GGNNCC 1 cut(s) 540
PstI CTGCAG 1 cut(s) 636
PsuI RGATCY 2 cut(s) 658, 1017
PsyI GACNNNGTC 1 cut(s) 833
PvuII CAGCTG 1 cut(s) 104
RseI CAYNNNNRTG 1 cut(s) 1397
SaqAI TTAA 1 cut(s) 1062
SatI GCNGC 4 cut(s) 105, 214, 632, 850
Sau3AI GATC 5 cut(s) 27, 609, 658, 816, 1017
ScaI AGTACT 4 cut(s) 373, 387, 1126, 1136
SchI GAGTC 3 cut(s) 102, 764, 773
ScrFI CCNGG 3 cut(s) 16, 353, 1167
SduI GDGCHC 2 cut(s) 204, 604
SfaNI GCATC 4 cut(s) 61, 200, 228, 352
SfcI CTRYAG 2 cut(s) 632, 1086
SfuI TTCGAA 1 cut(s) 639
SmiMI CAYNNNNRTG 1 cut(s) 1397
SmlI CTYRAG 1 cut(s) 947
SmoI CTYRAG 1 cut(s) 947
SnaBI TACGTA 1 cut(s) 421
SpeI ACTAGT 1 cut(s) 196
SsiI CCGC 6 cut(s) 397, 604, 706, 847, 1191, 1404
SspI AATATT 2 cut(s) 53, 67
SspMI CTAG 6 cut(s) 197, 489, 900, 1140, 1323, 1419
StyD4I CCNGG 3 cut(s) 14, 351, 1165
TaaI ACNGT 3 cut(s) 703, 1067, 1270
TaiI ACGT 3 cut(s) 423, 476, 1035
TaqI TCGA 8 cut(s) 35, 522, 612, 639, 648, 1151, 1461, 1473
TatI WGTACW 4 cut(s) 371, 385, 1124, 1134
TfiI GAWTC 8 cut(s) 60, 82, 393, 482, 530, 641, 794, 1076
Tru1I TTAA 1 cut(s) 1062
Tru9I TTAA 1 cut(s) 1062
TscAI CASTG 2 cut(s) 604, 991
TseFI GTSAC 2 cut(s) 827, 1270
TseI GCWGC 4 cut(s) 104, 213, 631, 849
Tsp45I GTSAC 2 cut(s) 827, 1270
TspDTI ATGAA 5 cut(s) 294, 347, 454, 502, 1347
TspRI CASTG 2 cut(s) 604, 991
Tth111I GACNNNGTC 1 cut(s) 833
VneI GTGCAC 1 cut(s) 200
XagI CCTNNNNNAGG 1 cut(s) 973
XapI RAATTY 2 cut(s) 155, 731
XceI RCATGY 1 cut(s) 566
XcmI CCANNNNNNNNNTGG 1 cut(s) 407
XmiI GTMKAC 1 cut(s) 1106
XmnI GAANNNNTTC 1 cut(s) 960
XspI CTAG 6 cut(s) 197, 489, 900, 1140, 1323, 1419
ZrmI AGTACT 4 cut(s) 373, 387, 1126, 1136
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.