FvH4_3g15930

divergent subfamily of APPLE domains

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0000207
Physical Location & Seq
Reverse (-)
10035372 .. 10039065
3694 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g15930.t1

Sequence Viewer

Length: 2562 bp
ATGCATGGAGCAAGCTTTAGCTTTTCCATTTTTACTTGTTTCAACTTCTCTGCATATATTATGCATCACTTTCTTTCAGTAAGAAATATACATTCTGGTCAGAAAATGCAAGCCTTTAGAACCCTTTTTGTATGCTCTTTTGTTTTCTCCTTCCTACAAACCTCATGTACAACTGCACTAGACACAATCACTCCAAGTCGGTATGTTAGAGATGGTGAAACTCTAGTTTCAGCTGATGGAAGCTTTGAATTGGGATTCTTTGCCAAGTCAAAAGGCCAATACTTGGGAATTTGGTACACTTTTTCTACCGACATAGTTGTATGGGTAGCCAACAGAGAAACACCAATAAGTGATTCTTCAGGAGTCTTAAAGCTCACTGATCAAGGAATTCTAGTGCTTCTGAATAGCTCAAACAGCACCATATGGTCATCCAATTCATCAAGAACCACAAATAATCCAGTATCACAACTCTTGGATTCGGGAAATCTTGTTGTGAAAGACAGAAATGATACTAGTCCTGATAATTTTCTTTGGCAGAGTTTTGATTATCCATGTGATACACAACTACAAGATATGAAACTTGGCTGGAACTTAGTTAATGGTTTAGATATGTATCTCTCGTCTTGGAGGAACACAGAAGATCCTGCTCAAGGAGAGTTTTCACTACGGATGGATACTCGTGGTTTACCTCAACTTTTTGAATTTAGTGGAGCTAAGATACTGACTAGAGGAGGATCATGGAACGGTCTTCAGTTAACTGGATACCCGAGGAGGCCAAATCCAATAGCTGAGTTTATATTTACATCGAATGAGACAGAAGTCTATTATGAGTACACACTCCTCAACCGCTCGACGTTTTCTAGATATGTATTAAATCCATCTGGCACCACACAGTGGTTAATATGGATAGACTACACACATAGTTGGGATGTTTTCTTCGCAAGCCAAGTAGATCAGTGTGAAAATTATGCCTTTTGTGGTGCCAATACTATTTGTAATGTTAATGCTGCTCCCATGTGTGCATGCTTGAAAGGATTTGTACCTGCATCTCCAGAAAACTGGAACTCAACAGGTTGGTCCGATGGATGTGTTCGAAAGACTACATTAGCTTGCAACTCTACAGATGGCTTCTCAAAGTACAGTAACTTTAAACTGCCAGACACATCTTCTTCCTGGTATGACAAGAGCATCAGCCTCAAGGAATGCAAGGGACTGTGTTTGAAAAATTGCTCATGTACGGCATATGCTAATTTAGATGTCAGGGAAGGGGGGAGCGGCTGCTTACTTTGGTTTGGAAACCTCACTGACATAAGAGAGTTCACCTCTGGTTCTCAAGACCTCTATATACGAATTGCTGCTTCAGACTTAGATGCTATTGGGAAGAAGAGCAAAACCAACAAGAAAAGGCAAGCTGCAATTGTAATCAGCTCTGCTCTTCTTGTGATGGGAATGCTAATACTTGGATGCCTTTTGTATAAACGAAAAAAGAAACTGAGCAATCAAGATGTCAGAAGATTGCTGGATTGCAGAAGGGATTACTTTGAAGAAGAAAGGGAAGACATGGAGTTACCACTCTTTGACCTGACCACTATAGCTGATGCCACTGATAACTTTTCAATCGACAACAAACTGGGAGAAGGTGGTTTTGGACCTGTTTACAAGGGTACATTAAAAGGAGGGGAAGAAATAGCTGTAAAGAGGCGTTCCAAGGATTCCGGACAAGGTGTGAGGGAGTTCAAAAATGAAGTTATACTGATAGCCAAACTCCAGCACCGGAATCTTGTAAAGCTTTTGGGTTGCTGCATTCATGATCAGGAAAAAATGTTGATATACGAATACATGTCCAACAGAAGTTTGGACTTCTTTATATTTGACCAGGAAAGACAAAAATCACTCGACTGGCCTACCTGCTACAACATTATTGAAGGAACAGCTCGAGGCCTTCTTTATCTTCACCAAGACTCAAGATTAAGGATTGTCCACAGAGATCTGAAACCAAGCAATGTCTTGCTTGATAAAGACATGAACCCAAAGATTTCAGACTTTGGTCTAGCTAAAACATTTAGCTTTGATCAAAGTCAAGCCAAAACGAATAAAGTGGCTGGAACATACGGTTATATGGCGCCTGAATATGCAGTAGATGGAATTTTCTCGATGAAATCAGATGTATTTAGCTTTGGAGTTGTAGTGATTGAGTTATTGAGTAAAGAGAAAAACAGGGGATTTTGTCATCCAGATCATGATTTTAATCTTCTTGGACATGCATGGACACTATGGACTCAAAATATACCGTTGGAACTCATCGACAAGACATTTTGTGATCCACAAACCATATCCGAAGTGTTAAGATGTCTCCATGTGGGTCTGTTATGTGTGCAACAAGTACCTGAAGATAGACCAAGCATGTCCTCCGTGGTTCTAATGTTGGGCAGTGATGTTATGCTGCCTGTGCCAAAGCAGCCTGGTTTTTACACTGAACGGACTCTACCAGAATCATCATCAAGGTCATGCGACCGGTGTTCACCAAACAACTTCAGCACTACATTGCTAGAGGCGCGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

854

Amino Acids

96.24

Weight (kDa)

6.18

Isoelectric Point (pI)

45.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 106 - 210 4.3e-37 D-mannose binding lectin
S_locus_glycop PF00954 243 - 349 2.3e-24 S-locus glycoprotein domain
PAN_2 PF08276 371 - 437 7.4e-22 PAN-like domain
PK_Tyr_Ser-Thr PF07714 539 - 806 3.7e-46 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 539 - 805 3.5e-45 Protein kinase domain
DUF3403 PF11883 813 - 853 2.7e-06 Domain of unknown function (DUF3403)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000207)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G27300
fragaria_vesca FvH4_3g15930 FvH4_3g15980 FvH4_3g15980 FvH4_3g15980 FvH4_3g15980 FvH4_3g21350 FvH4_3g21400 FvH4_3g21400 FvH4_6g12332
malus_domestica MD02G1167100.v1.1 MD02G1167200.v1.1 MD03G1185600.v1.1 MD05G1213900.v1.1 MD05G1214100.v1.1 MD05G1214200.v1.1 MD05G1214700.v1.1 MD05G1216300.v1.1 MD05G1216800.v1.1 MD05G1217300.v1.1 MD05G1218000.v1.1 MD11G1231100.v1.1 MD11G1231200.v1.1 MD11G1231400.v1.1 MD11G1231500.v1.1 MD11G1232400.v1.1 MD11G1232500.v1.1
prunus_persica Prupe.4G142300_v2.0.a1 Prupe.4G142400_v2.0.a1 Prupe.4G142500_v2.0.a1 Prupe.4G142600_v2.0.a1 Prupe.4G142800_v2.0.a1 Prupe.4G195000_v2.0.a1 Prupe.4G195000_v2.0.a1 Prupe.4G195000_v2.0.a1 Prupe.4G195100_v2.0.a1 Prupe.4G195300_v2.0.a1 Prupe.4G195300_v2.0.a1 Prupe.4G195300_v2.0.a1 Prupe.4G195400_v2.0.a1 Prupe.4G195400_v2.0.a1 Prupe.4G195500_v2.0.a1 Prupe.4G195700_v2.0.a1 Prupe.4G195900_v2.0.a1
pyrus_communis pycom03g16540 pycom05g19920 pycom11g20460 pycom11g20480 pycom11g20490 pycom11g20500 pycom11g20530
rosa_chinensis RchiOBHm_Chr2g0139311 RchiOBHm_Chr2g0139351 RchiOBHm_Chr5g0026801 RchiOBHm_Chr5g0026851 RchiOBHm_Chr5g0026891 RchiOBHm_Chr5g0026911 RchiOBHm_Chr5g0036571 RchiOBHm_Chr5g0036591 RchiOBHm_Chr5g0036721 RchiOBHm_Chr5g0036741 RchiOBHm_Chr7g0222981
rosa_laevigata RLG00000002006 RLG00000019807 RLG00000032964 RLG00000032968 RLG00000032969 RLG00000033706 RLG00000033708 RLG00000033712 RLG00000033716
rosa_multiflora Rmu_co8256651.1_g000001 Rmu_co8279377.1_g000001 Rmu_co8429919.1_g000001 Rmu_co8490103.1_g000001 Rmu_sc0000774.1_g000002 Rmu_sc0002525.1_g000012 Rmu_sc0004223.1_g000008 Rmu_sc0004390.1_g000002 Rmu_sc0006059.1_g000014 Rmu_sc0006059.1_g000024 Rmu_sc0006514.1_g000020 Rmu_sc0014333.1_g000002 Rmu_ssc0000172.1_g000018 Rmu_ssc0000172.1_g000029
rosa_roxburghii Rroxscaffold_1G00044230 Rroxscaffold_1G00044260 Rroxscaffold_1G00044320 Rroxscaffold_1G00044340 Rroxscaffold_1G00052560 Rroxscaffold_1G00052570 Rroxscaffold_1G00052660 Rroxscaffold_1G00052720 Rroxscaffold_2G00105450 Rroxscaffold_2G00105460
rosa_rugosa Rorug02G0356900 Rorug02G0357000 Rorug02G0357100 Rorug02G0357200 Rorug02G0357300 Rorug02G0357400 Rorug02G0357500 Rorug02G0357600 Rorug05G0095400 Rorug05G0095500 Rorug05G0095500 Rorug05G0095600 Rorug05G0095700 Rorug05G0095800 Rorug05G0157700 Rorug05G0157800
rosa_samantha Rh2AG406800 Rh2AG406900 Rh2BG417300 Rh2CG392900 Rh2DG426200 Rh5AG189300 Rh5AG249400 Rh5AG250100 Rh5BG186300 Rh5BG186900 Rh5BG187000 Rh5BG249500 Rh5BG250600 Rh5BG250900 Rh5BG251500 Rh5BG251800 Rh5CG205500 Rh5CG206300 Rh5CG206700 Rh5CG283000 Rh5CG283300 Rh5CG283500 Rh5DG188500 Rh5DG188700 Rh5DG258700 Rh5DG258900 Rh5DG259600 Rh5DG259900 Rh7CG375800 Rh7DG352500
rosa_wichuraiana Rw2G032960 Rw2G032970 Rw2G033220 Rw2G033230 Rw5G017130 Rw5G017230 Rw5G022940 Rw5G022960 Rw5G022980 Rw7G030420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 1051, 1916
AccB1I GGYRCC 3 cut(s) 884, 980, 2122
AccB7I CCANNNNNTGG 2 cut(s) 283, 894
AccBSI CCGCTC 2 cut(s) 849, 1275
AccII CGCG 1 cut(s) 2557
AccIII TCCGGA 1 cut(s) 1715
AciI CCGC 3 cut(s) 847, 1275, 2557
AclWI GGATC 3 cut(s) 635, 742, 2315
AcsI RAATTY 4 cut(s) 288, 387, 701, 2145
AcuI CTGAAG 5 cut(s) 342, 734, 1344, 2409, 2518
AcyI GRCGYC 1 cut(s) 2123
AdeI CACNNNGTG 1 cut(s) 894
AfaI GTAC 8 cut(s) 169, 296, 833, 1041, 1139, 1237, 1666, 2385
AfiI CCNNNNNNNGG 3 cut(s) 283, 650, 894
AflIII ACRYGT 1 cut(s) 1839
AgeI ACCGGT 1 cut(s) 2514
AgsI TTSAA 9 cut(s) 43, 248, 701, 1030, 1222, 1544, 1617, 1738, 1925
AhlI ACTAGT 1 cut(s) 512
AjnI CCWGG 3 cut(s) 1172, 1875, 2461
AjuI GAANNNNNNNTTGG 4 cut(s) 337, 369, 1629, 1661
Alw26I GTCTC 2 cut(s) 806, 2357
AlwI GGATC 3 cut(s) 635, 742, 2315
Ama87I CYCGRG 2 cut(s) 766, 1935
Aor13HI TCCGGA 1 cut(s) 1715
AoxI GGCC 4 cut(s) 274, 773, 1901, 1939
ApeKI GCWGC 7 cut(s) 1007, 1278, 1355, 1412, 1800, 2443, 2458
ApoI RAATTY 4 cut(s) 288, 387, 701, 2145
AsiGI ACCGGT 1 cut(s) 2514
AspLEI GCGC 2 cut(s) 2125, 2557
AspS9I GGNCC 2 cut(s) 1077, 1649
AsuHPI GGTGA 4 cut(s) 227, 1312, 1946, 2514
AsuII TTCGAA 1 cut(s) 1093
AvaI CYCGRG 2 cut(s) 766, 1935
AvaII GGWCC 2 cut(s) 1077, 1649
BanI GGYRCC 3 cut(s) 884, 980, 2122
BauI CACGAG 1 cut(s) 678
BbsI GAAGAC 2 cut(s) 740, 1563
BbvI GCAGC 7 cut(s) 994, 1265, 1342, 1399, 1787, 2430, 2470
BccI CCATC 8 cut(s) 206, 230, 664, 886, 1076, 1118, 1438, 2135
BceAI ACGGC 1 cut(s) 1254
BciT130I CCWGG 3 cut(s) 1174, 1877, 2463
BciVI GTATCC 2 cut(s) 667, 755
BclI TGATCA 3 cut(s) 379, 1810, 2071
BcoDI GTCTC 2 cut(s) 806, 2357
BcuI ACTAGT 1 cut(s) 512
BfaI CTAG 8 cut(s) 179, 224, 392, 513, 726, 861, 2051, 2549
BfmI CTRYAG 2 cut(s) 1119, 1590
BfoI RGCGCY 1 cut(s) 2126
BfuAI ACCTGC 2 cut(s) 1051, 1916
BfuI GTATCC 2 cut(s) 667, 755
BglII AGATCT 1 cut(s) 1987
BisI GCNGC 8 cut(s) 1008, 1276, 1279, 1356, 1413, 1801, 2444, 2459
BlsI GCNGC 8 cut(s) 1009, 1277, 1280, 1357, 1414, 1802, 2445, 2460
Bme1390I CCNGG 3 cut(s) 1174, 1877, 2463
Bme18I GGWCC 2 cut(s) 1077, 1649
BmeT110I CYCGRG 2 cut(s) 766, 1935
BmgT120I GGNCC 2 cut(s) 1077, 1649
BmiI GGNNCC 3 cut(s) 886, 982, 2124
BmrFI CCNGG 3 cut(s) 1174, 1877, 2463
BmrI ACTGGG 1 cut(s) 1640
BmsI GCATC 6 cut(s) 73, 1055, 1197, 1360, 1454, 1588
BmuI ACTGGG 1 cut(s) 1640
BoxI GACNNNNGTC 2 cut(s) 818, 2046
BpiI GAAGAC 2 cut(s) 740, 1563
BplI GAGNNNNNCTC 2 cut(s) 1307, 1339
BpmI CTGGAG 2 cut(s) 1035, 1751
Bpu14I TTCGAA 1 cut(s) 1093
BpuEI CTTGAG 4 cut(s) 633, 1181, 1317, 1948
BsaBI GATNNNNATC 2 cut(s) 612, 2247
BsaHI GRCGYC 1 cut(s) 2123
BsaJI CCNNGG 3 cut(s) 767, 1707, 2412
BsaWI WCCGGW 3 cut(s) 1715, 1773, 2514
BsaXI ACNNNNNCTCC 4 cut(s) 175, 205, 2172, 2202
Bsc4I CCNNNNNNNGG 3 cut(s) 283, 650, 894
Bse118I RCCGGY 1 cut(s) 2514
Bse1I ACTGG 5 cut(s) 458, 763, 1064, 1635, 1904
Bse3DI GCAATG 2 cut(s) 2008, 2543
Bse8I GATNNNNATC 2 cut(s) 612, 2247
BseAI TCCGGA 1 cut(s) 1715
BseBI CCWGG 3 cut(s) 1174, 1877, 2463
BseDI CCNNGG 3 cut(s) 767, 1707, 2412
BseGI GGATG 6 cut(s) 428, 675, 934, 1091, 1469, 2230
BseJI GATNNNNATC 2 cut(s) 612, 2247
BseLI CCNNNNNNNGG 3 cut(s) 283, 650, 894
BseMI GCAATG 2 cut(s) 2008, 2543
BseMII CTCAG 2 cut(s) 780, 1484
BseNI ACTGG 5 cut(s) 458, 763, 1064, 1635, 1904
BseRI GAGGAG 3 cut(s) 744, 784, 830
BseXI GCAGC 7 cut(s) 994, 1265, 1342, 1399, 1787, 2430, 2470
BsgI GTGCAG 1 cut(s) 159
Bsh1236I CGCG 1 cut(s) 2557
Bsh1285I CGRYCG 1 cut(s) 2515
BshFI GGCC 4 cut(s) 276, 775, 1903, 1941
BshNI GGYRCC 3 cut(s) 884, 980, 2122
BshTI ACCGGT 1 cut(s) 2514
BsiEI CGRYCG 1 cut(s) 2515
BsiHKCI CYCGRG 2 cut(s) 766, 1935
BsiSI CCGG 3 cut(s) 1716, 1774, 2515
BslFI GGGAC 1 cut(s) 1224
BslI CCNNNNNNNGG 3 cut(s) 283, 650, 894
BsmAI GTCTC 2 cut(s) 806, 2357
BsmFI GGGAC 1 cut(s) 1224
BsmI GAATGC 3 cut(s) 1208, 1455, 1803
BsnI GGCC 4 cut(s) 276, 775, 1903, 1941
BsoBI CYCGRG 2 cut(s) 766, 1935
Bsp119I TTCGAA 1 cut(s) 1093
Bsp13I TCCGGA 1 cut(s) 1715
Bsp1407I TGTACA 1 cut(s) 167
Bsp143I GATC 9 cut(s) 379, 640, 734, 952, 1810, 1987, 2071, 2236, 2320
BspACI CCGC 3 cut(s) 847, 1275, 2557
BspANI GGCC 4 cut(s) 276, 775, 1903, 1941
BspCNI CTCAG 2 cut(s) 781, 1485
BspEI TCCGGA 1 cut(s) 1715
BspFNI CGCG 1 cut(s) 2557
BspHI TCATGA 2 cut(s) 1807, 2239
BspLI GGNNCC 3 cut(s) 886, 982, 2124
BspMI ACCTGC 2 cut(s) 1051, 1916
BspPI GGATC 3 cut(s) 635, 742, 2315
BspQI GCTCTTC 2 cut(s) 1379, 1440
BspT104I TTCGAA 1 cut(s) 1093
BspT107I GGYRCC 3 cut(s) 884, 980, 2122
BsrBI CCGCTC 2 cut(s) 849, 1275
BsrDI GCAATG 2 cut(s) 2008, 2543
BsrFI RCCGGY 1 cut(s) 2514
BsrGI TGTACA 1 cut(s) 167
BsrI ACTGG 5 cut(s) 458, 763, 1064, 1635, 1904
BssAI RCCGGY 1 cut(s) 2514
BssECI CCNNGG 3 cut(s) 767, 1707, 2412
BssMI GATC 9 cut(s) 379, 640, 734, 952, 1810, 1987, 2071, 2236, 2320
BssNI GRCGYC 1 cut(s) 2123
BssSI CACGAG 1 cut(s) 678
BssT1I CCWWGG 1 cut(s) 1707
Bst2BI CACGAG 1 cut(s) 678
Bst2UI CCWGG 3 cut(s) 1174, 1877, 2463
Bst4CI ACNGT 6 cut(s) 746, 894, 1142, 1215, 2114, 2292
Bst6I CTCTTC 2 cut(s) 1379, 1440
BstACI GRCGYC 1 cut(s) 2123
BstAUI TGTACA 1 cut(s) 167
BstBI TTCGAA 1 cut(s) 1093
BstC8I GCNNGC 6 cut(s) 13, 111, 943, 1024, 1111, 1410
BstDEI CTNAG 5 cut(s) 592, 714, 789, 1366, 1493
BstDSI CCRYGG 1 cut(s) 2412
BstENI CCTNNNNNAGG 1 cut(s) 648
BstF5I GGATG 6 cut(s) 428, 675, 934, 1091, 1469, 2230
BstFNI CGCG 1 cut(s) 2557
BstH2I RGCGCY 1 cut(s) 2126
BstHHI GCGC 2 cut(s) 2125, 2557
BstKTI GATC 9 cut(s) 382, 643, 737, 955, 1813, 1990, 2074, 2239, 2323
BstMAI GTCTC 2 cut(s) 806, 2357
BstMBI GATC 9 cut(s) 379, 640, 734, 952, 1810, 1987, 2071, 2236, 2320
BstMCI CGRYCG 1 cut(s) 2515
BstMWI GCNNNNNNNGC 4 cut(s) 414, 2449, 2458, 2554
BstNI CCWGG 3 cut(s) 1174, 1877, 2463
BstNSI RCATGY 4 cut(s) 1026, 1843, 2264, 2407
BstPAI GACNNNNGTC 2 cut(s) 818, 2046
BstSCI CCNGG 3 cut(s) 1172, 1875, 2461
BstSFI CTRYAG 2 cut(s) 1119, 1590
BstUI CGCG 1 cut(s) 2557
BstV1I GCAGC 7 cut(s) 994, 1265, 1342, 1399, 1787, 2430, 2470
BstV2I GAAGAC 2 cut(s) 740, 1563
BstX2I RGATCY 2 cut(s) 640, 1987
BstXI CCANNNNNNTGG 1 cut(s) 1059
BstYI RGATCY 2 cut(s) 640, 1987
BsuI GTATCC 2 cut(s) 667, 755
BsuRI GGCC 4 cut(s) 276, 775, 1903, 1941
BtgI CCRYGG 1 cut(s) 2412
BtsCI GGATG 6 cut(s) 428, 675, 934, 1091, 1469, 2230
BtsI GCAGTG 1 cut(s) 2437
BtsIMutI CAGTG 7 cut(s) 375, 899, 962, 1302, 1602, 2437, 2472
BveI ACCTGC 2 cut(s) 1051, 1916
Cac8I GCNNGC 6 cut(s) 13, 111, 943, 1024, 1111, 1410
CciI TCATGA 2 cut(s) 1807, 2239
CfoI GCGC 2 cut(s) 2125, 2557
Cfr10I RCCGGY 1 cut(s) 2514
Cfr13I GGNCC 2 cut(s) 1077, 1649
Csp6I GTAC 8 cut(s) 168, 295, 832, 1040, 1138, 1236, 1665, 2384
CspAI ACCGGT 1 cut(s) 2514
CviQI GTAC 8 cut(s) 168, 295, 832, 1040, 1138, 1236, 1665, 2384
DdeI CTNAG 5 cut(s) 592, 714, 789, 1366, 1493
DinI GGCGCC 1 cut(s) 2124
DpnI GATC 9 cut(s) 381, 642, 736, 954, 1812, 1989, 2073, 2238, 2322
DpnII GATC 9 cut(s) 379, 640, 734, 952, 1810, 1987, 2071, 2236, 2320
DraI TTTAAA 1 cut(s) 1150
DraIII CACNNNGTG 1 cut(s) 894
Eam1104I CTCTTC 2 cut(s) 1379, 1440
EarI CTCTTC 2 cut(s) 1379, 1440
Eco130I CCWWGG 1 cut(s) 1707
Eco147I AGGCCT 1 cut(s) 1941
Eco47I GGWCC 2 cut(s) 1077, 1649
Eco57I CTGAAG 5 cut(s) 342, 734, 1344, 2409, 2518
Eco88I CYCGRG 2 cut(s) 766, 1935
EcoNI CCTNNNNNAGG 1 cut(s) 648
EcoRI GAATTC 1 cut(s) 387
EcoRII CCWGG 3 cut(s) 1172, 1875, 2461
EcoT14I CCWWGG 1 cut(s) 1707
EcoT22I ATGCAT 3 cut(s) 6, 66, 2266
EgeI GGCGCC 1 cut(s) 2124
EheI GGCGCC 1 cut(s) 2124
ErhI CCWWGG 1 cut(s) 1707
FalI AAGNNNNNCTT 2 cut(s) 340, 372
FaqI GGGAC 1 cut(s) 1224
FauNDI CATATG 2 cut(s) 422, 1243
FbaI TGATCA 3 cut(s) 379, 1810, 2071
Fnu4HI GCNGC 8 cut(s) 1008, 1276, 1279, 1356, 1413, 1801, 2444, 2459
FokI GGATG 6 cut(s) 415, 682, 941, 1098, 1476, 2217
Fsp4HI GCNGC 8 cut(s) 1008, 1276, 1279, 1356, 1413, 1801, 2444, 2459
FspBI CTAG 8 cut(s) 179, 224, 392, 513, 726, 861, 2051, 2549
GlaI GCGC 2 cut(s) 2124, 2556
GluI GCNGC 8 cut(s) 1008, 1276, 1279, 1356, 1413, 1801, 2444, 2459
GsuI CTGGAG 2 cut(s) 1035, 1751
HaeII RGCGCY 1 cut(s) 2126
HaeIII GGCC 4 cut(s) 276, 775, 1903, 1941
HapII CCGG 3 cut(s) 1716, 1774, 2515
HhaI GCGC 2 cut(s) 2125, 2557
Hin1I GRCGYC 1 cut(s) 2123
Hin6I GCGC 2 cut(s) 2123, 2555
HinP1I GCGC 2 cut(s) 2123, 2555
HincII GTYRAC 1 cut(s) 756
HindII GTYRAC 1 cut(s) 756
HindIII AAGCTT 3 cut(s) 13, 241, 1787
HpaI GTTAAC 1 cut(s) 756
HpaII CCGG 3 cut(s) 1716, 1774, 2515
HphI GGTGA 4 cut(s) 227, 1312, 1946, 2514
Hpy166II GTNNAC 8 cut(s) 297, 686, 756, 834, 1320, 1657, 1981, 2522
Hpy188I TCNGA 9 cut(s) 102, 402, 1081, 1363, 1511, 1992, 2041, 2164, 2338
Hpy8I GTNNAC 8 cut(s) 297, 686, 756, 834, 1320, 1657, 1981, 2522
Hpy99I CGWCG 1 cut(s) 856
HpyAV CCTTC 6 cut(s) 160, 1259, 1524, 1631, 1919, 1952
HpyCH4III ACNGT 6 cut(s) 746, 894, 1142, 1215, 2114, 2292
HpyCH4IV ACGT 1 cut(s) 854
HpyF10VI GCNNNNNNNGC 4 cut(s) 414, 2449, 2458, 2554
HpyF3I CTNAG 5 cut(s) 592, 714, 789, 1366, 1493
HpySE526I ACGT 1 cut(s) 854
Hsp92I GRCGYC 1 cut(s) 2123
HspAI GCGC 2 cut(s) 2123, 2555
KasI GGCGCC 1 cut(s) 2122
Kpn2I TCCGGA 1 cut(s) 1715
Ksp22I TGATCA 3 cut(s) 379, 1810, 2071
KspAI GTTAAC 1 cut(s) 756
Kzo9I GATC 9 cut(s) 379, 640, 734, 952, 1810, 1987, 2071, 2236, 2320
LguI GCTCTTC 2 cut(s) 1379, 1440
LmnI GCTCC 4 cut(s) 8, 710, 1015, 1272
Lsp1109I GCAGC 7 cut(s) 994, 1265, 1342, 1399, 1787, 2430, 2470
LweI GCATC 6 cut(s) 73, 1055, 1197, 1360, 1454, 1588
MaeI CTAG 8 cut(s) 179, 224, 392, 513, 726, 861, 2051, 2549
MaeII ACGT 1 cut(s) 854
MaeIII GTNAC 2 cut(s) 1142, 1566
MalI GATC 9 cut(s) 381, 642, 736, 954, 1812, 1989, 2073, 2238, 2322
MbiI CCGCTC 2 cut(s) 849, 1275
MboI GATC 9 cut(s) 379, 640, 734, 952, 1810, 1987, 2071, 2236, 2320
MfeI CAATTG 1 cut(s) 1416
MflI RGATCY 2 cut(s) 640, 1987
Mly113I GGCGCC 1 cut(s) 2123
MlyI GAGTC 4 cut(s) 372, 1955, 2272, 2476
MmeI TCCRAC 2 cut(s) 1869, 2274
Mph1103I ATGCAT 3 cut(s) 6, 66, 2266
MroI TCCGGA 1 cut(s) 1715
MspA1I CMGCKG 1 cut(s) 233
MspI CCGG 3 cut(s) 1716, 1774, 2515
MspR9I CCNGG 3 cut(s) 1174, 1877, 2463
MunI CAATTG 1 cut(s) 1416
Mva1269I GAATGC 3 cut(s) 1208, 1455, 1803
MvaI CCWGG 3 cut(s) 1174, 1877, 2463
MvnI CGCG 1 cut(s) 2557
MwoI GCNNNNNNNGC 4 cut(s) 414, 2449, 2458, 2554
NarI GGCGCC 1 cut(s) 2123
NdeI CATATG 2 cut(s) 422, 1243
NdeII GATC 9 cut(s) 379, 640, 734, 952, 1810, 1987, 2071, 2236, 2320
NlaIV GGNNCC 3 cut(s) 886, 982, 2124
NsiI ATGCAT 3 cut(s) 6, 66, 2266
NspI RCATGY 4 cut(s) 1026, 1843, 2264, 2407
NspV TTCGAA 1 cut(s) 1093
PaeI GCATGC 1 cut(s) 1026
PaeR7I CTCGAG 1 cut(s) 1935
PagI TCATGA 2 cut(s) 1807, 2239
PceI AGGCCT 1 cut(s) 1941
PciI ACATGT 1 cut(s) 1839
PciSI GCTCTTC 2 cut(s) 1379, 1440
PctI GAATGC 3 cut(s) 1208, 1455, 1803
PfeI GAWTC 6 cut(s) 255, 353, 476, 1712, 1777, 2492
PflMI CCANNNNNTGG 2 cut(s) 283, 894
PinAI ACCGGT 1 cut(s) 2514
PkrI GCNGC 8 cut(s) 1009, 1277, 1280, 1357, 1414, 1802, 2445, 2460
PleI GAGTC 4 cut(s) 371, 1955, 2272, 2476
PluTI GGCGCC 1 cut(s) 2126
PpsI GAGTC 4 cut(s) 371, 1955, 2272, 2476
PscI ACATGT 1 cut(s) 1839
PshAI GACNNNNGTC 2 cut(s) 818, 2046
Psp6I CCWGG 3 cut(s) 1172, 1875, 2461
PspGI CCWGG 3 cut(s) 1172, 1875, 2461
PspN4I GGNNCC 3 cut(s) 886, 982, 2124
PspPI GGNCC 2 cut(s) 1077, 1649
PspXI VCTCGAGB 1 cut(s) 1935
PsrI GAACNNNNNNTAC 2 cut(s) 2469, 2501
PsuI RGATCY 2 cut(s) 640, 1987
PvuII CAGCTG 1 cut(s) 233
RsaI GTAC 8 cut(s) 169, 296, 833, 1041, 1139, 1237, 1666, 2385
RsaNI GTAC 8 cut(s) 168, 295, 832, 1040, 1138, 1236, 1665, 2384
SapI GCTCTTC 2 cut(s) 1379, 1440
SatI GCNGC 8 cut(s) 1008, 1276, 1279, 1356, 1413, 1801, 2444, 2459
Sau3AI GATC 9 cut(s) 379, 640, 734, 952, 1810, 1987, 2071, 2236, 2320
Sau96I GGNCC 2 cut(s) 1077, 1649
SchI GAGTC 4 cut(s) 372, 1955, 2272, 2476
ScrFI CCNGG 3 cut(s) 1174, 1877, 2463
SfaNI GCATC 6 cut(s) 73, 1055, 1197, 1360, 1454, 1588
SfcI CTRYAG 2 cut(s) 1119, 1590
SfoI GGCGCC 1 cut(s) 2124
Sfr274I CTCGAG 1 cut(s) 1935
SfuI TTCGAA 1 cut(s) 1093
SinI GGWCC 2 cut(s) 1077, 1649
SlaI CTCGAG 1 cut(s) 1935
SmlI CTYRAG 5 cut(s) 648, 1196, 1332, 1935, 1963
SmoI CTYRAG 5 cut(s) 648, 1196, 1332, 1935, 1963
SpeI ACTAGT 1 cut(s) 512
SphI GCATGC 1 cut(s) 1026
SseBI AGGCCT 1 cut(s) 1941
SsiI CCGC 3 cut(s) 847, 1275, 2557
SspDI GGCGCC 1 cut(s) 2122
SspMI CTAG 8 cut(s) 179, 224, 392, 513, 726, 861, 2051, 2549
StuI AGGCCT 1 cut(s) 1941
StyD4I CCNGG 3 cut(s) 1172, 1875, 2461
StyI CCWWGG 1 cut(s) 1707
TaaI ACNGT 6 cut(s) 746, 894, 1142, 1215, 2114, 2292
TaiI ACGT 1 cut(s) 857
TaqI TCGA 8 cut(s) 806, 851, 1093, 1620, 1896, 1936, 2153, 2304
TatI WGTACW 3 cut(s) 167, 831, 1137
TauI GCSGC 1 cut(s) 1278
TfiI GAWTC 6 cut(s) 255, 353, 476, 1712, 1777, 2492
TscAI CASTG 7 cut(s) 382, 899, 962, 1309, 1609, 2437, 2479
TseI GCWGC 7 cut(s) 1007, 1278, 1355, 1412, 1800, 2443, 2458
TspDTI ATGAA 6 cut(s) 426, 590, 1758, 1796, 2039, 2171
TspGWI ACGGA 3 cut(s) 682, 2401, 2494
TspRI CASTG 7 cut(s) 382, 899, 962, 1309, 1609, 2437, 2479
Van91I CCANNNNNTGG 2 cut(s) 283, 894
VpaK11BI GGWCC 2 cut(s) 1077, 1649
XagI CCTNNNNNAGG 1 cut(s) 648
XapI RAATTY 4 cut(s) 288, 387, 701, 2145
XbaI TCTAGA 1 cut(s) 860
XceI RCATGY 4 cut(s) 1026, 1843, 2264, 2407
XcmI CCANNNNNNNNNTGG 1 cut(s) 1852
XhoI CTCGAG 1 cut(s) 1935
XspI CTAG 8 cut(s) 179, 224, 392, 513, 726, 861, 2051, 2549
Zsp2I ATGCAT 3 cut(s) 6, 66, 2266
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.