MD05G1213900.v1.1

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0000207
Physical Location & Seq
Reverse (-)
34522675 .. 34525893
3219 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1213900.v1.1.491

Sequence Viewer

Length: 2424 bp
ATGCAAGCCTTGAGAAGCTTTTTTATGTGCTTGTTCTTCTTATTCTCCCTTCTAAGAATCTCAGCTGCAACTGCACTGGGCACAATCACTCCGAGTGGATATATAAGAGATGGCGAAACTCTGGTTTCAGTAGGTGGAAGCTATGAACTGGGATTCTTCAGCCCTGGTAAATCGAAAAGCCGGTACTTGGGAATATGGTACACTGTTTCTACCGATGCAGTTGTGTGGGTAGCCAACAGAGAAACACCACTTGGTGATTCTTCAGGACTTTTGAAGCTCACGGAGCAAGGAGTTCTAGTCATCCTGAATAGCTCAAACAGCATTGTGTGGTCATCGAATTCATCAAGAACTGCGGGGAATCTAGTATCGCAACTATTGGATTCTGGAAATCTTGTGGTGCAAGATGGGAAAGAAACTAACCTGGATAACTTCTTGTGGCAGAGTTTTGATTATCCTTGTGACACATACCTACCGGAAATGAAGCTTGGTTGGGACTTGGTTGCCGGTTTAGAGAGGTATCTCTCGTCTTGGAAGAGCACAGAAGATCCTGCTCCGGGAGAGTTTTCGGAACGAGTGGATCTGCGTGGCTTACCACAATTTTTTATTATGAAAGGAGCTAAGATACAAACCAGACGAGGGTCATGGAATGGCCTTTATTTTACAGGATCCGAATATGGTGTCAGAGAACCAAATCCAATATCTGGGACTAAATTTGTGATGAACAAGGATGAGGTCTCTTTGAAGTACAGGCTAAAGAACATGTCAGTGTTCTCGAGATATGTATTGAACTCATTAGGAGTCGCAGAGAAGTTCACATGGGTGTACCAAACACAGAGTTGGGAACTTGCCTCTACATTCCTAGCAGATCGGTGTGAAAGTTATGCCTTGTGTGGTCCATTTTCTAGTTGCAAAAGCAATGGTAACTCTGCAATATGTGCATGCTTGAAGGGATTTGTACCAAAATCTTTGAAAGATTGGAATTCGGGATATTGGTCTGATGGATGTGTTCGAAGGACTCTGTTGACCTGCAGCCATGGAGATGGCTTCACAAAGTACAGTGGGATTAAATTGCCAGACACATCAACCTCGTGGTATGATAAAAGCACGAGCCTTAAAGAATGCAAGGGATTATGTTTGGGAAACTGCTTATGTACTGCGTGTGCAAATCTAGACATCAGGGAGAGAGGAAGTGGCTGCGTGCTTTGGTTTGGTAACCTCATCGACATAAAAGAATTCACTCCTGGCGGTGGTCAAGACCTCTATATACGGGTGGCAGCTTCAGAACTAGATATTTCAGATCATGTTAAGAAAATGAGCAATTTCAACAAGAGAAAGTTACTAGCAATTCTCCTCAGCTCTGCAATTTTTCTCATGGGACTGCTAATAGTTGGAATAATATTGTACATACAGAAGAAGAAACTTAGAAATGAAGGACACAGAAGAAAGGATTGCAGTGACGAGTACGTCGGAGAAGACGGGGAAGAGATGGAGTTACCACTGTTTGACTTGACCACCATAGCTAAAGCCACTGACAACTTTTCAAGCCGCAACAAGCTGGGAGAAGGTGGCTTTGGACCTGTGTACAAGGGAATAAGAGAGTTCAAAACTGAAGTTATTCTCATAGCCAGACTTCAACATCGCAATCTTGTTAAGCTTCTCGGTTGTTGCATTCAAAAAGACGAAAAGATTCTAATATATGAATTCCTGCCCAACAAAAGCTTGGACTTCTATATTTTTGATCAAGAGGGTCAAAAATTGCTCGACTGGCCTAAGTGCTTCCACATTATCAGTGGAGTTGCTCGAGGGCTTCTTTATCTTCACCAAGACTCTAGATTAAGGATTATACATCGAGATCTGAAATGTAGTAATATTCTGCTAGATAATAATATGAACCCGAAGATTTCAGACTTTGGCCTGGCTAAAACATTTGGCGGTGACCAAAGTCAAGCCAATACAAACAGAGTGGTTGGAACCTATGGTTATATGTCTCCGGAATATGCAGCAGATGGAACTTTCTCGATGAAATCTGATGTGTTTAGCTTGGGAGTCATACTGCTCGAGATGTTGAGCAGGAGGAAAAACAGGGGATTTCGTCATCCAGATCACGACCACAACCTTCTTGGACATGCATGGATACTATGGATTCAAGATAAACCACTAGAACTGATCAATAAGACATTTTGTGATTCATGCAACATATCTGAAGTGATAAGGTGTTTTCATGTGGGGCTATTATGTGTGCAAAGAGTACCTGAGGATAGACCAAGCATGTCATCTGTGGTTTTGATGTTGAGCAGTGGTGATGTCTTGCCGCCTCCAAAGCAGCCTGGTTTTTACATTGAACGAAGCGTCCATGAATCACCAACAAGGTCGCTTCCATGTTCAGAGAATACTTTCAGCACTACGATGATAGAAGCTCGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

808

Amino Acids

90.49

Weight (kDa)

8.25

Isoelectric Point (pI)

43.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 74 - 178 3e-37 D-mannose binding lectin
S_locus_glycop PF00954 211 - 322 5.2e-24 S-locus glycoprotein domain
PAN_2 PF08276 343 - 409 9.5e-19 PAN-like domain
PK_Tyr_Ser-Thr PF07714 532 - 760 1.4e-34 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 534 - 760 7.3e-37 Protein kinase domain
DUF3403 PF11883 769 - 807 7.8e-07 Domain of unknown function (DUF3403)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000207)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G27300
fragaria_vesca FvH4_3g15930 FvH4_3g15980 FvH4_3g15980 FvH4_3g15980 FvH4_3g15980 FvH4_3g21350 FvH4_3g21400 FvH4_3g21400 FvH4_6g12332
malus_domestica MD02G1167100.v1.1 MD02G1167200.v1.1 MD03G1185600.v1.1 MD05G1213900.v1.1 MD05G1214100.v1.1 MD05G1214200.v1.1 MD05G1214700.v1.1 MD05G1216300.v1.1 MD05G1216800.v1.1 MD05G1217300.v1.1 MD05G1218000.v1.1 MD11G1231100.v1.1 MD11G1231200.v1.1 MD11G1231400.v1.1 MD11G1231500.v1.1 MD11G1232400.v1.1 MD11G1232500.v1.1
prunus_persica Prupe.4G142300_v2.0.a1 Prupe.4G142400_v2.0.a1 Prupe.4G142500_v2.0.a1 Prupe.4G142600_v2.0.a1 Prupe.4G142800_v2.0.a1 Prupe.4G195000_v2.0.a1 Prupe.4G195000_v2.0.a1 Prupe.4G195000_v2.0.a1 Prupe.4G195100_v2.0.a1 Prupe.4G195300_v2.0.a1 Prupe.4G195300_v2.0.a1 Prupe.4G195300_v2.0.a1 Prupe.4G195400_v2.0.a1 Prupe.4G195400_v2.0.a1 Prupe.4G195500_v2.0.a1 Prupe.4G195700_v2.0.a1 Prupe.4G195900_v2.0.a1
pyrus_communis pycom03g16540 pycom05g19920 pycom11g20460 pycom11g20480 pycom11g20490 pycom11g20500 pycom11g20530
rosa_chinensis RchiOBHm_Chr2g0139311 RchiOBHm_Chr2g0139351 RchiOBHm_Chr5g0026801 RchiOBHm_Chr5g0026851 RchiOBHm_Chr5g0026891 RchiOBHm_Chr5g0026911 RchiOBHm_Chr5g0036571 RchiOBHm_Chr5g0036591 RchiOBHm_Chr5g0036721 RchiOBHm_Chr5g0036741 RchiOBHm_Chr7g0222981
rosa_laevigata RLG00000002006 RLG00000019807 RLG00000032964 RLG00000032968 RLG00000032969 RLG00000033706 RLG00000033708 RLG00000033712 RLG00000033716
rosa_multiflora Rmu_co8256651.1_g000001 Rmu_co8279377.1_g000001 Rmu_co8429919.1_g000001 Rmu_co8490103.1_g000001 Rmu_sc0000774.1_g000002 Rmu_sc0002525.1_g000012 Rmu_sc0004223.1_g000008 Rmu_sc0004390.1_g000002 Rmu_sc0006059.1_g000014 Rmu_sc0006059.1_g000024 Rmu_sc0006514.1_g000020 Rmu_sc0014333.1_g000002 Rmu_ssc0000172.1_g000018 Rmu_ssc0000172.1_g000029
rosa_roxburghii Rroxscaffold_1G00044230 Rroxscaffold_1G00044260 Rroxscaffold_1G00044320 Rroxscaffold_1G00044340 Rroxscaffold_1G00052560 Rroxscaffold_1G00052570 Rroxscaffold_1G00052660 Rroxscaffold_1G00052720 Rroxscaffold_2G00105450 Rroxscaffold_2G00105460
rosa_rugosa Rorug02G0356900 Rorug02G0357000 Rorug02G0357100 Rorug02G0357200 Rorug02G0357300 Rorug02G0357400 Rorug02G0357500 Rorug02G0357600 Rorug05G0095400 Rorug05G0095500 Rorug05G0095500 Rorug05G0095600 Rorug05G0095700 Rorug05G0095800 Rorug05G0157700 Rorug05G0157800
rosa_samantha Rh2AG406800 Rh2AG406900 Rh2BG417300 Rh2CG392900 Rh2DG426200 Rh5AG189300 Rh5AG249400 Rh5AG250100 Rh5BG186300 Rh5BG186900 Rh5BG187000 Rh5BG249500 Rh5BG250600 Rh5BG250900 Rh5BG251500 Rh5BG251800 Rh5CG205500 Rh5CG206300 Rh5CG206700 Rh5CG283000 Rh5CG283300 Rh5CG283500 Rh5DG188500 Rh5DG188700 Rh5DG258700 Rh5DG258900 Rh5DG259600 Rh5DG259900 Rh7CG375800 Rh7DG352500
rosa_wichuraiana Rw2G032960 Rw2G032970 Rw2G033220 Rw2G033230 Rw5G017130 Rw5G017230 Rw5G022940 Rw5G022960 Rw5G022980 Rw7G030420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1463
Acc36I ACCTGC 1 cut(s) 1034
AccB7I CCANNNNNTGG 1 cut(s) 701
AccIII TCCGGA 1 cut(s) 1990
AciI CCGC 5 cut(s) 353, 1245, 1546, 1932, 2312
AclWI GGATC 4 cut(s) 539, 585, 660, 673
AcsI RAATTY 5 cut(s) 337, 710, 979, 1232, 1700
AcuI CTGAAG 5 cut(s) 142, 246, 1263, 1629, 2223
AdeI CACNNNGTG 1 cut(s) 254
AfiI CCNNNNNNNGG 5 cut(s) 187, 554, 636, 701, 1247
AflIII ACRYGT 1 cut(s) 759
AjnI CCWGG 5 cut(s) 163, 420, 1240, 1914, 2326
AjuI GAANNNNNNNTTGG 6 cut(s) 234, 266, 468, 500, 1554, 1586
AleI CACNNNNGTG 1 cut(s) 818
AloI GAACNNNNNNTCC 2 cut(s) 2334, 2366
Alw21I GWGCWC 1 cut(s) 539
Alw26I GTCTC 2 cut(s) 739, 1992
AlwI GGATC 4 cut(s) 539, 585, 660, 673
Ama87I CYCGRG 3 cut(s) 772, 1800, 2057
Aor13HI TCCGGA 1 cut(s) 1990
AoxI GGCC 3 cut(s) 649, 1766, 1912
ApeKI GCWGC 6 cut(s) 65, 1029, 1194, 1274, 2000, 2323
ApoI RAATTY 5 cut(s) 337, 710, 979, 1232, 1700
ArsI GACNNNNNNTTYG 2 cut(s) 663, 695
Asp700I GAANNNNTTC 3 cut(s) 1614, 1686, 2393
AspS9I GGNCC 2 cut(s) 893, 1574
AsuC2I CCSGG 1 cut(s) 555
AsuHPI GGTGA 5 cut(s) 266, 1811, 1946, 2312, 2352
AsuII TTCGAA 1 cut(s) 1009
AvaI CYCGRG 3 cut(s) 772, 1800, 2057
AvaII GGWCC 2 cut(s) 893, 1574
AxyI CCTNAGG 1 cut(s) 2253
BaeGI GKGCMC 1 cut(s) 83
BamHI GGATCC 1 cut(s) 665
BauI CACGAG 2 cut(s) 1087, 1105
BbsI GAAGAC 1 cut(s) 1479
Bbv12I GWGCWC 1 cut(s) 539
BbvCI CCTCAGC 1 cut(s) 1352
BbvI GCAGC 6 cut(s) 52, 1041, 1181, 1286, 2012, 2335
BccI CCATC 6 cut(s) 104, 398, 992, 1034, 1480, 2000
BciT130I CCWGG 5 cut(s) 165, 422, 1242, 1916, 2328
BciVI GTATCC 1 cut(s) 2127
BclI TGATCA 2 cut(s) 1738, 2166
BcnI CCSGG 1 cut(s) 555
BcoDI GTCTC 2 cut(s) 739, 1992
BfmI CTRYAG 1 cut(s) 1027
BfuAI ACCTGC 1 cut(s) 1034
BfuI GTATCC 1 cut(s) 2127
BglII AGATCT 1 cut(s) 1852
BisI GCNGC 8 cut(s) 66, 1030, 1195, 1275, 1546, 2001, 2312, 2324
BlsI GCNGC 8 cut(s) 67, 1031, 1196, 1276, 1547, 2002, 2313, 2325
Bme1390I CCNGG 6 cut(s) 165, 422, 555, 1242, 1916, 2328
Bme18I GGWCC 2 cut(s) 893, 1574
BmeT110I CYCGRG 3 cut(s) 772, 1800, 2057
BmgT120I GGNCC 2 cut(s) 893, 1574
BmiI GGNNCC 2 cut(s) 667, 1972
BmrFI CCNGG 6 cut(s) 165, 422, 555, 1242, 1916, 2328
BmrI ACTGGG 2 cut(s) 86, 158
BmsI GCATC 1 cut(s) 205
BmuI ACTGGG 2 cut(s) 86, 158
BoxI GACNNNNGTC 2 cut(s) 637, 1941
BpiI GAAGAC 1 cut(s) 1479
Bpu10I CCTNAGC 1 cut(s) 1352
Bpu14I TTCGAA 1 cut(s) 1009
BpuEI CTTGAG 1 cut(s) 31
BpuMI CCSGG 1 cut(s) 555
BsaI GGTCTC 1 cut(s) 739
BsaJI CCNNGG 2 cut(s) 163, 1033
BsaWI WCCGGW 2 cut(s) 472, 1990
BsaXI ACNNNNNCTCC 4 cut(s) 73, 103, 282, 312
Bsc4I CCNNNNNNNGG 5 cut(s) 187, 554, 636, 701, 1247
Bse118I RCCGGY 2 cut(s) 180, 503
Bse1I ACTGG 3 cut(s) 81, 153, 1769
Bse21I CCTNAGG 1 cut(s) 2253
Bse3DI GCAATG 1 cut(s) 922
BseAI TCCGGA 1 cut(s) 1990
BseBI CCWGG 5 cut(s) 165, 422, 1242, 1916, 2328
BseDI CCNNGG 2 cut(s) 163, 1033
BseGI GGATG 4 cut(s) 300, 733, 1007, 2095
BseLI CCNNNNNNNGG 5 cut(s) 187, 554, 636, 701, 1247
BseMI GCAATG 1 cut(s) 922
BseMII CTCAG 3 cut(s) 75, 1366, 2244
BseNI ACTGG 3 cut(s) 81, 153, 1769
BseRI GAGGAG 1 cut(s) 1340
BseSI GKGCMC 1 cut(s) 83
BseXI GCAGC 6 cut(s) 52, 1041, 1181, 1286, 2012, 2335
BseYI CCCAGC 1 cut(s) 1555
BsgI GTGCAG 1 cut(s) 57
BshFI GGCC 3 cut(s) 651, 1768, 1914
BsiHKAI GWGCWC 1 cut(s) 539
BsiHKCI CYCGRG 3 cut(s) 772, 1800, 2057
BsiSI CCGG 5 cut(s) 181, 473, 504, 554, 1991
BslFI GGGAC 3 cut(s) 506, 718, 1389
BslI CCNNNNNNNGG 5 cut(s) 187, 554, 636, 701, 1247
BsmAI GTCTC 2 cut(s) 739, 1992
BsmFI GGGAC 3 cut(s) 506, 718, 1389
BsmI GAATGC 2 cut(s) 1124, 1668
BsnI GGCC 3 cut(s) 651, 1768, 1914
Bso31I GGTCTC 1 cut(s) 739
BsoBI CYCGRG 3 cut(s) 772, 1800, 2057
Bsp119I TTCGAA 1 cut(s) 1009
Bsp1286I GDGCHC 2 cut(s) 83, 539
Bsp13I TCCGGA 1 cut(s) 1990
Bsp1407I TGTACA 2 cut(s) 1401, 1581
Bsp143I GATC 9 cut(s) 544, 577, 665, 865, 1297, 1738, 1852, 2101, 2166
Bsp19I CCATGG 1 cut(s) 1033
BspACI CCGC 5 cut(s) 353, 1245, 1546, 1932, 2312
BspANI GGCC 3 cut(s) 651, 1768, 1914
BspCNI CTCAG 3 cut(s) 74, 1365, 2245
BspEI TCCGGA 1 cut(s) 1990
BspLI GGNNCC 2 cut(s) 667, 1972
BspMAI CTGCAG 1 cut(s) 1031
BspMI ACCTGC 1 cut(s) 1034
BspPI GGATC 4 cut(s) 539, 585, 660, 673
BspQI GCTCTTC 1 cut(s) 527
BspT104I TTCGAA 1 cut(s) 1009
BspTNI GGTCTC 1 cut(s) 739
BsrDI GCAATG 1 cut(s) 922
BsrFI RCCGGY 2 cut(s) 180, 503
BsrGI TGTACA 2 cut(s) 1401, 1581
BsrI ACTGG 3 cut(s) 81, 153, 1769
BssAI RCCGGY 2 cut(s) 180, 503
BssECI CCNNGG 2 cut(s) 163, 1033
BssMI GATC 9 cut(s) 544, 577, 665, 865, 1297, 1738, 1852, 2101, 2166
BssSI CACGAG 2 cut(s) 1087, 1105
BssT1I CCWWGG 1 cut(s) 1033
Bst2BI CACGAG 2 cut(s) 1087, 1105
Bst2UI CCWGG 5 cut(s) 165, 422, 1242, 1916, 2328
Bst4CI ACNGT 3 cut(s) 205, 1058, 1500
Bst6I CTCTTC 2 cut(s) 527, 1476
BstAPI GCANNNNNTGC 1 cut(s) 935
BstAUI TGTACA 2 cut(s) 1401, 1581
BstBI TTCGAA 1 cut(s) 1009
BstC8I GCNNGC 3 cut(s) 6, 940, 1199
BstDEI CTNAG 7 cut(s) 53, 61, 618, 1352, 1421, 1770, 2253
BstDSI CCRYGG 1 cut(s) 1033
BstEII GGTNACC 2 cut(s) 1211, 1934
BstF5I GGATG 4 cut(s) 300, 733, 1007, 2095
BstKTI GATC 9 cut(s) 547, 580, 668, 868, 1300, 1741, 1855, 2104, 2169
BstMAI GTCTC 2 cut(s) 739, 1992
BstMBI GATC 9 cut(s) 544, 577, 665, 865, 1297, 1738, 1852, 2101, 2166
BstMWI GCNNNNNNNGC 6 cut(s) 71, 283, 318, 935, 1765, 2320
BstNI CCWGG 5 cut(s) 165, 422, 1242, 1916, 2328
BstNSI RCATGY 4 cut(s) 763, 942, 2129, 2272
BstPAI GACNNNNGTC 2 cut(s) 637, 1941
BstPI GGTNACC 2 cut(s) 1211, 1934
BstSCI CCNGG 6 cut(s) 163, 420, 553, 1240, 1914, 2326
BstSFI CTRYAG 1 cut(s) 1027
BstSLI GKGCMC 1 cut(s) 83
BstV1I GCAGC 6 cut(s) 52, 1041, 1181, 1286, 2012, 2335
BstV2I GAAGAC 1 cut(s) 1479
BstX2I RGATCY 4 cut(s) 544, 577, 665, 1852
BstXI CCANNNNNNTGG 1 cut(s) 1040
BstYI RGATCY 4 cut(s) 544, 577, 665, 1852
Bsu36I CCTNAGG 1 cut(s) 2253
BsuI GTATCC 1 cut(s) 2127
BsuRI GGCC 3 cut(s) 651, 1768, 1914
BtgI CCRYGG 1 cut(s) 1033
BtgZI GCGATG 1 cut(s) 1622
BtsCI GGATG 4 cut(s) 300, 733, 1007, 2095
BtsI GCAGTG 2 cut(s) 1459, 2302
BtsIMutI CAGTG 9 cut(s) 74, 201, 771, 1063, 1459, 1496, 1527, 1795, 2302
BveI ACCTGC 1 cut(s) 1034
Cac8I GCNNGC 3 cut(s) 6, 940, 1199
Cfr10I RCCGGY 2 cut(s) 180, 503
Cfr13I GGNCC 2 cut(s) 893, 1574
CseI GACGC 1 cut(s) 2338
CspCI CAANNNNNGTGG 2 cut(s) 1944, 1979
DdeI CTNAG 7 cut(s) 53, 61, 618, 1352, 1421, 1770, 2253
DpnI GATC 9 cut(s) 546, 579, 667, 867, 1299, 1740, 1854, 2103, 2168
DpnII GATC 9 cut(s) 544, 577, 665, 865, 1297, 1738, 1852, 2101, 2166
DraIII CACNNNGTG 1 cut(s) 254
DrdI GACNNNNNNGTC 1 cut(s) 1463
DseDI GACNNNNNNGTC 1 cut(s) 1463
Eam1104I CTCTTC 2 cut(s) 527, 1476
EarI CTCTTC 2 cut(s) 527, 1476
Eco130I CCWWGG 1 cut(s) 1033
Eco31I GGTCTC 1 cut(s) 739
Eco47I GGWCC 2 cut(s) 893, 1574
Eco57I CTGAAG 5 cut(s) 142, 246, 1263, 1629, 2223
Eco81I CCTNAGG 1 cut(s) 2253
Eco88I CYCGRG 3 cut(s) 772, 1800, 2057
Eco91I GGTNACC 2 cut(s) 1211, 1934
EcoO65I GGTNACC 2 cut(s) 1211, 1934
EcoRI GAATTC 4 cut(s) 337, 979, 1232, 1700
EcoRII CCWGG 5 cut(s) 163, 420, 1240, 1914, 2326
EcoT14I CCWWGG 1 cut(s) 1033
EcoT22I ATGCAT 1 cut(s) 2131
ErhI CCWWGG 1 cut(s) 1033
FaqI GGGAC 3 cut(s) 506, 718, 1389
FauI CCCGC 1 cut(s) 346
FbaI TGATCA 2 cut(s) 1738, 2166
Fnu4HI GCNGC 8 cut(s) 66, 1030, 1195, 1275, 1546, 2001, 2312, 2324
FokI GGATG 4 cut(s) 287, 740, 1014, 2082
Fsp4HI GCNGC 8 cut(s) 66, 1030, 1195, 1275, 1546, 2001, 2312, 2324
GluI GCNGC 8 cut(s) 66, 1030, 1195, 1275, 1546, 2001, 2312, 2324
GsaI CCCAGC 1 cut(s) 1559
HaeIII GGCC 3 cut(s) 651, 1768, 1914
HapII CCGG 5 cut(s) 181, 473, 504, 554, 1991
HgaI GACGC 1 cut(s) 2338
HincII GTYRAC 1 cut(s) 1023
HindII GTYRAC 1 cut(s) 1023
HindIII AAGCTT 4 cut(s) 16, 482, 1652, 1717
HpaII CCGG 5 cut(s) 181, 473, 504, 554, 1991
HphI GGTGA 5 cut(s) 266, 1811, 1946, 2312, 2352
Hpy166II GTNNAC 5 cut(s) 201, 813, 823, 1023, 1582
Hpy8I GTNNAC 5 cut(s) 201, 813, 823, 1023, 1582
Hpy99I CGWCG 1 cut(s) 1469
HpyAV CCTTC 6 cut(s) 59, 940, 1005, 1424, 1556, 2126
HpyCH4III ACNGT 3 cut(s) 205, 1058, 1500
HpyCH4IV ACGT 1 cut(s) 1464
HpyF10VI GCNNNNNNNGC 6 cut(s) 71, 283, 318, 935, 1765, 2320
HpyF3I CTNAG 7 cut(s) 53, 61, 618, 1352, 1421, 1770, 2253
HpySE526I ACGT 1 cut(s) 1464
Kpn2I TCCGGA 1 cut(s) 1990
Ksp22I TGATCA 2 cut(s) 1738, 2166
Kzo9I GATC 9 cut(s) 544, 577, 665, 865, 1297, 1738, 1852, 2101, 2166
LguI GCTCTTC 1 cut(s) 527
LmnI GCTCC 3 cut(s) 283, 556, 614
Lsp1109I GCAGC 6 cut(s) 52, 1041, 1181, 1286, 2012, 2335
LweI GCATC 1 cut(s) 205
MaeII ACGT 1 cut(s) 1464
MaeIII GTNAC 7 cut(s) 458, 920, 1211, 1335, 1454, 1491, 1934
MalI GATC 9 cut(s) 546, 579, 667, 867, 1299, 1740, 1854, 2103, 2168
MboI GATC 9 cut(s) 544, 577, 665, 865, 1297, 1738, 1852, 2101, 2166
MflI RGATCY 4 cut(s) 544, 577, 665, 1852
MhlI GDGCHC 2 cut(s) 83, 539
MlyI GAGTC 4 cut(s) 807, 1009, 1820, 2055
MmeI TCCRAC 3 cut(s) 1369, 1447, 1948
Mph1103I ATGCAT 1 cut(s) 2131
MroI TCCGGA 1 cut(s) 1990
MroXI GAANNNNTTC 3 cut(s) 1614, 1686, 2393
MseI TTAA 5 cut(s) 1065, 1113, 1305, 1650, 1835
MslI CAYNNNNRTG 4 cut(s) 764, 818, 1038, 2405
MspA1I CMGCKG 1 cut(s) 65
MspI CCGG 5 cut(s) 181, 473, 504, 554, 1991
MspR9I CCNGG 6 cut(s) 165, 422, 555, 1242, 1916, 2328
Mva1269I GAATGC 2 cut(s) 1124, 1668
MvaI CCWGG 5 cut(s) 165, 422, 1242, 1916, 2328
MwoI GCNNNNNNNGC 6 cut(s) 71, 283, 318, 935, 1765, 2320
NciI CCSGG 1 cut(s) 555
NcoI CCATGG 1 cut(s) 1033
NdeII GATC 9 cut(s) 544, 577, 665, 865, 1297, 1738, 1852, 2101, 2166
NlaIV GGNNCC 2 cut(s) 667, 1972
NmuCI GTSAC 3 cut(s) 458, 1454, 1934
NsiI ATGCAT 1 cut(s) 2131
NspI RCATGY 4 cut(s) 763, 942, 2129, 2272
NspV TTCGAA 1 cut(s) 1009
OliI CACNNNNGTG 1 cut(s) 818
PaeI GCATGC 1 cut(s) 942
PaeR7I CTCGAG 3 cut(s) 772, 1800, 2057
PciI ACATGT 1 cut(s) 759
PciSI GCTCTTC 1 cut(s) 527
PctI GAATGC 2 cut(s) 1124, 1668
PdmI GAANNNNTTC 3 cut(s) 1614, 1686, 2393
PfeI GAWTC 9 cut(s) 57, 153, 257, 358, 380, 1687, 2143, 2186, 2357
PflMI CCANNNNNTGG 1 cut(s) 701
PfoI TCCNGGA 1 cut(s) 553
PkrI GCNGC 8 cut(s) 67, 1031, 1196, 1276, 1547, 2002, 2313, 2325
PleI GAGTC 4 cut(s) 806, 1009, 1820, 2054
PpsI GAGTC 4 cut(s) 806, 1009, 1820, 2054
PscI ACATGT 1 cut(s) 759
PshAI GACNNNNGTC 2 cut(s) 637, 1941
Psp6I CCWGG 5 cut(s) 163, 420, 1240, 1914, 2326
PspEI GGTNACC 2 cut(s) 1211, 1934
PspFI CCCAGC 1 cut(s) 1555
PspGI CCWGG 5 cut(s) 163, 420, 1240, 1914, 2326
PspN4I GGNNCC 2 cut(s) 667, 1972
PspPI GGNCC 2 cut(s) 893, 1574
PspXI VCTCGAGB 1 cut(s) 1800
PstI CTGCAG 1 cut(s) 1031
PsuI RGATCY 4 cut(s) 544, 577, 665, 1852
PvuII CAGCTG 1 cut(s) 65
RseI CAYNNNNRTG 4 cut(s) 764, 818, 1038, 2405
SapI GCTCTTC 1 cut(s) 527
SaqAI TTAA 5 cut(s) 1065, 1113, 1305, 1650, 1835
SatI GCNGC 8 cut(s) 66, 1030, 1195, 1275, 1546, 2001, 2312, 2324
Sau3AI GATC 9 cut(s) 544, 577, 665, 865, 1297, 1738, 1852, 2101, 2166
Sau96I GGNCC 2 cut(s) 893, 1574
SchI GAGTC 4 cut(s) 807, 1009, 1820, 2055
ScrFI CCNGG 6 cut(s) 165, 422, 555, 1242, 1916, 2328
SduI GDGCHC 2 cut(s) 83, 539
SfaNI GCATC 1 cut(s) 205
SfcI CTRYAG 1 cut(s) 1027
Sfr274I CTCGAG 3 cut(s) 772, 1800, 2057
SfuI TTCGAA 1 cut(s) 1009
SinI GGWCC 2 cut(s) 893, 1574
SlaI CTCGAG 3 cut(s) 772, 1800, 2057
SmiMI CAYNNNNRTG 4 cut(s) 764, 818, 1038, 2405
SmlI CTYRAG 4 cut(s) 10, 772, 1800, 2057
SmoI CTYRAG 4 cut(s) 10, 772, 1800, 2057
SphI GCATGC 1 cut(s) 942
SsiI CCGC 5 cut(s) 353, 1245, 1546, 1932, 2312
SspI AATATT 2 cut(s) 1398, 1870
StyD4I CCNGG 6 cut(s) 163, 420, 553, 1240, 1914, 2326
StyI CCWWGG 1 cut(s) 1033
TaaI ACNGT 3 cut(s) 205, 1058, 1500
TaiI ACGT 1 cut(s) 1467
TatI WGTACW 5 cut(s) 744, 1053, 1151, 1401, 1581
TauI GCSGC 2 cut(s) 1548, 2314
TfiI GAWTC 9 cut(s) 57, 153, 257, 358, 380, 1687, 2143, 2186, 2357
Tru1I TTAA 5 cut(s) 1065, 1113, 1305, 1650, 1835
Tru9I TTAA 5 cut(s) 1065, 1113, 1305, 1650, 1835
TscAI CASTG 9 cut(s) 81, 208, 771, 1063, 1459, 1503, 1534, 1795, 2302
TseFI GTSAC 3 cut(s) 458, 1454, 1934
TseI GCWGC 6 cut(s) 65, 1029, 1194, 1274, 2000, 2323
Tsp45I GTSAC 3 cut(s) 458, 1454, 1934
TspGWI ACGGA 1 cut(s) 296
TspRI CASTG 9 cut(s) 81, 208, 771, 1063, 1459, 1503, 1534, 1795, 2302
Van91I CCANNNNNTGG 1 cut(s) 701
VpaK11BI GGWCC 2 cut(s) 893, 1574
XapI RAATTY 5 cut(s) 337, 710, 979, 1232, 1700
XbaI TCTAGA 2 cut(s) 1168, 1829
XceI RCATGY 4 cut(s) 763, 942, 2129, 2272
XcmI CCANNNNNNNNNTGG 3 cut(s) 1717, 1787, 2117
XhoI CTCGAG 3 cut(s) 772, 1800, 2057
XmnI GAANNNNTTC 3 cut(s) 1614, 1686, 2393
Zsp2I ATGCAT 1 cut(s) 2131
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.