Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr7

Orthogroup ID

OG0000207

Physical Location & Seq

Genomic Coordinates Forward (+)

43197952 .. 43201187

Gene Structure 3236 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw7G030420.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2430 bp

ATGGATGATCTTTGTTTTGTGGTGTTTAGTACTAGTTTGTTCTCTCTGTTGTTCACAGGTTCGTTTGCAGTTGACAACATCAATCCGTCGCAGTCTATCAGTGATAATGGCAGCGCCTTGGTTTCAAGTGATGGAACATTTGAGTTGGGTTTCTTCAGTCCAGGTAACACGAAACATCGCTACTTGGGAATTTGGTACAAAAACATCCCAGTTAAAACTGTTGTTTGGGTTGCAAATCGATGCAAACCGATTGATGACTCATCCGGTGTGTTGATGATAAACAGCACAGGATATCTTGTGCTGTTGGGTCAGAATAAGACTGTTGTTTGGTGGACAAGTTTAGCTAAGTATGCACAGAGTACAATGGTACAGCTATTAGATTCTGGAAATTTAGTAGTGAGGGATGTGACAGATGGAAAGTCATTGTGGCAAAGCTTCGATTATCCTTCTGATACATTGTTACCAGAAATGAAGATGGGATGGGACTTTAGGACAGGTCTTCAGAGGGGATTATCAGCATGGAAAAATTCAGAAGACCCATGTCCAGGGGAATTCACTTATGGGATTGAAATGGAACCTCATGCATACCCTGAGGCATATTTTCGGAAAGGTGGTCTGAAATTTTATCGTTCTGGCCCATGGAATGGCCTAAGATTCAGTGGCGCAGCAGCTCTAAGGCCTAATCCAATTTATAGTTTCGATTTTGTGCATAATGATGATGAAGTTTACTACATGTACAAACTTCAAAGCAAGTCTGTAATTTCAATCGTGGTCTTAAATGGAACGACGAGTACGCGCCATCGCCTTACATGGATTGAAGCAGAGCACACTTGGAGGGCCTATGCAATAGTACCTAGAGATTTCTGTGATCAATATGGCCTCTGTGGAGCAAATGCAGAGTGTATCGTTAGTAACAGTCCAGTATGTCAATGTCTACAAGGATTCACGCCCAAGTCACCAGAGAAATGGAATTTGACAGACTGGTCACTTGGATGTACGCGCAAGAAACCCCTAAGCTGCCAGGAAAGTGATAAAGACGGGTTTGTCAAATTCACTGACTTGAAATTGCCAGATACTACACATTCTTGGGTGAACAAAAGTACGAACATCAAGGAATGCAGAGCCAAATGCTTGAACAACTGTTCCTGTATGGCTTATACAAGCTCGGACATTAAAGCAGGAGGTTCTGGCTGCGCCATTTGGTTTGGTGATCTACTAGATATCAGAAAGATTCCGGATGCTGGCCAAGATCTATACATTCGAATGTCAGCTTCAGAGAAAGAAGATGATGGTAAAGTGAAGACAGGAATTATAGTTTCTGTTGTGATAGCTGCAGTGTTTACAGGAATAATCTTAATTGGCTACTACGTGTACCGAAGCAGGACCAAATTAAAAGAAAAACGAGAAGAAGGGGCACAAAAGGAGGACCTGAAGCTCCCGCTTTTCAACATAACCACGATAGCTACTGCTACAGATAACTTTTCAGATGATAAGAAGCTTGGAGAAGGTGGCTTTGGACCTGTTTACAAGGGGACACTAGCAGATGGACAAGAATTTGCTGTGAAGAGGCTTTCAAGCAGTTCTGGCCAAGGATTTAATGAGTTTAGGAATGAAATTATAATGATTGCCAAACTTCAGCACCGAAATCTTGTAAAGATTCTTGGTTGTTGTGTGCAAGAGGAGAAAATGCTGATGTACGAATACATGTCCAACGGAAGCCTGGACTACTTCATTTTTGATCAAACCAGGGAAGAACTACTGTTAGATTGGCCTAAACGTTTCCACATCATTTGTGGAATTGCTCGGGGTCTCCTCTATCTCCACCAAAATTCCAGGCTAAGGATTATACATAGAGATCTAAAAGCAAGTAATGTTCTACTTGATGATGAAATGAATCCTAAAATTTCAGACTTTGGCTTGGCTAGAATATTAACCGGAGGAGATCAGTCTGGAGGAAATACGAAGAGAGTGGTCGGGACATATGGCTACATGGCACCTGAATATGCTGCTGATGGGGTATTTTCTGTGAAATCCGATGTCTTTAGCTTCGGTATATTGGTGCTAGAGATTATAAGTGGAAGGAGAAACAAAGGATTTTATCATCCAGACGACAGCCATAATCTTATTGGAAATGCTTGGAGATTGTGGAATGAAGGAAGGCCTTTAGAATTGATTGATTCGTGGTTAAAGAGCTTATGTGATCTATCAGAAGTGTTACGTTGCATCCAAGTTAGTCTTTTGTGCGTTCAACATCATCCTGAGAATCGGCCGAGCATGGCGTCTGTGGTTATAATGTTGGGAAGTGATATTGCATTGGCTCAACCTAAACAACCTGGTTTCCTTCTGGAAAATGAACATGAAGCAAGCCCTGATATAGGTAATGAATTATCGACCAATGAAATATCCATTTCGCTTTTGGAAGCGCGGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005886 GO:0005911 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0008150 GO:0008152 GO:0009506 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0019899 GO:0023052 GO:0030054 GO:0031625 GO:0036211 GO:0038023 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044389 GO:0044424 GO:0044444 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0055044 GO:0060089 GO:0065007 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

B_lectin DUF3403 PAN_2 Pkinase_Tyr S_locus_glycop

Protein Analysis

809

Amino Acids

90.62

Weight (kDa)

6.08

Isoelectric Point (pI)

41.17

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
B_lectin	PF01453	73 - 175	9.9e-36	D-mannose binding lectin
S_locus_glycop	PF00954	209 - 319	6.7e-28	S-locus glycoprotein domain
PAN_2	PF08276	340 - 408	6.1e-22	PAN-like domain
Pkinase	PF00069	495 - 761	5.8e-44	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	497 - 763	6.1e-47	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0000207)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G27300
fragaria_vesca	FvH4_3g15930 FvH4_3g15980 FvH4_3g15980 FvH4_3g15980 FvH4_3g15980 FvH4_3g21350 FvH4_3g21400 FvH4_3g21400 FvH4_6g12332
malus_domestica	MD02G1167100.v1.1 MD02G1167200.v1.1 MD03G1185600.v1.1 MD05G1213900.v1.1 MD05G1214100.v1.1 MD05G1214200.v1.1 MD05G1214700.v1.1 MD05G1216300.v1.1 MD05G1216800.v1.1 MD05G1217300.v1.1 MD05G1218000.v1.1 MD11G1231100.v1.1 MD11G1231200.v1.1 MD11G1231400.v1.1 MD11G1231500.v1.1 MD11G1232400.v1.1 MD11G1232500.v1.1
prunus_persica	Prupe.4G142300_v2.0.a1 Prupe.4G142400_v2.0.a1 Prupe.4G142500_v2.0.a1 Prupe.4G142600_v2.0.a1 Prupe.4G142800_v2.0.a1 Prupe.4G195000_v2.0.a1 Prupe.4G195000_v2.0.a1 Prupe.4G195000_v2.0.a1 Prupe.4G195100_v2.0.a1 Prupe.4G195300_v2.0.a1 Prupe.4G195300_v2.0.a1 Prupe.4G195300_v2.0.a1 Prupe.4G195400_v2.0.a1 Prupe.4G195400_v2.0.a1 Prupe.4G195500_v2.0.a1 Prupe.4G195700_v2.0.a1 Prupe.4G195900_v2.0.a1
pyrus_communis	pycom03g16540 pycom05g19920 pycom11g20460 pycom11g20480 pycom11g20490 pycom11g20500 pycom11g20530
rosa_chinensis	RchiOBHm_Chr2g0139311 RchiOBHm_Chr2g0139351 RchiOBHm_Chr5g0026801 RchiOBHm_Chr5g0026851 RchiOBHm_Chr5g0026891 RchiOBHm_Chr5g0026911 RchiOBHm_Chr5g0036571 RchiOBHm_Chr5g0036591 RchiOBHm_Chr5g0036721 RchiOBHm_Chr5g0036741 RchiOBHm_Chr7g0222981
rosa_laevigata	RLG00000002006 RLG00000019807 RLG00000032964 RLG00000032968 RLG00000032969 RLG00000033706 RLG00000033708 RLG00000033712 RLG00000033716
rosa_multiflora	Rmu_co8256651.1_g000001 Rmu_co8279377.1_g000001 Rmu_co8429919.1_g000001 Rmu_co8490103.1_g000001 Rmu_sc0000774.1_g000002 Rmu_sc0002525.1_g000012 Rmu_sc0004223.1_g000008 Rmu_sc0004390.1_g000002 Rmu_sc0006059.1_g000014 Rmu_sc0006059.1_g000024 Rmu_sc0006514.1_g000020 Rmu_sc0014333.1_g000002 Rmu_ssc0000172.1_g000018 Rmu_ssc0000172.1_g000029
rosa_roxburghii	Rroxscaffold_1G00044230 Rroxscaffold_1G00044260 Rroxscaffold_1G00044320 Rroxscaffold_1G00044340 Rroxscaffold_1G00052560 Rroxscaffold_1G00052570 Rroxscaffold_1G00052660 Rroxscaffold_1G00052720 Rroxscaffold_2G00105450 Rroxscaffold_2G00105460
rosa_rugosa	Rorug02G0356900 Rorug02G0357000 Rorug02G0357100 Rorug02G0357200 Rorug02G0357300 Rorug02G0357400 Rorug02G0357500 Rorug02G0357600 Rorug05G0095400 Rorug05G0095500 Rorug05G0095500 Rorug05G0095600 Rorug05G0095700 Rorug05G0095800 Rorug05G0157700 Rorug05G0157800
rosa_samantha	Rh2AG406800 Rh2AG406900 Rh2BG417300 Rh2CG392900 Rh2DG426200 Rh5AG189300 Rh5AG249400 Rh5AG250100 Rh5BG186300 Rh5BG186900 Rh5BG187000 Rh5BG249500 Rh5BG250600 Rh5BG250900 Rh5BG251500 Rh5BG251800 Rh5CG205500 Rh5CG206300 Rh5CG206700 Rh5CG283000 Rh5CG283300 Rh5CG283500 Rh5DG188500 Rh5DG188700 Rh5DG258700 Rh5DG258900 Rh5DG259600 Rh5DG259900 Rh7CG375800 Rh7DG352500
rosa_wichuraiana	Rw2G032960 Rw2G032970 Rw2G033220 Rw2G033230 Rw5G017130 Rw5G017230 Rw5G022940 Rw5G022960 Rw5G022980 Rw7G030420

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	1619, 2072, 2291
AasI	GACNNNNNNGTC	2 cut(s)	982, 1043
AccB1I	GGYRCC	1 cut(s)	1993
AccB7I	CCANNNNNTGG	1 cut(s)	644
AccI	GTMKAC	1 cut(s)	934
AccII	CGCG	3 cut(s)	796, 1000, 2425
AccIII	TCCGGA	1 cut(s)	1234
AciI	CCGC	2 cut(s)	1439, 2425
AclI	AACGTT	1 cut(s)	1777
AcoI	YGGCCR	3 cut(s)	1243, 1585, 2267
AcuI	CTGAAG	5 cut(s)	139, 485, 1257, 1451, 1619
AcyI	GRCGYC	1 cut(s)	2279
AfiI	CCNNNNNNNGG	5 cut(s)	545, 644, 1241, 1839, 2375
AflIII	ACRYGT	3 cut(s)	732, 1368, 1704
AhlI	ACTAGT	1 cut(s)	32
AjnI	CCWGG	7 cut(s)	160, 544, 1020, 1719, 1745, 1832, 2332
AjuI	GAANNNNNNNTTGG	2 cut(s)	1497, 1529
AloI	GAACNNNNNNTCC	2 cut(s)	1127, 1159
Alw21I	GWGCWC	1 cut(s)	828
Alw26I	GTCTC	1 cut(s)	1814
Ama87I	CYCGRG	1 cut(s)	1803
Aor13HI	TCCGGA	1 cut(s)	1234
ApeKI	GCWGC	7 cut(s)	111, 665, 668, 1017, 1191, 1331, 2006
AspLEI	GCGC	6 cut(s)	116, 665, 798, 1002, 1196, 2425
AspS9I	GGNCC	5 cut(s)	635, 837, 1383, 1426, 1517
AsuHPI	GGTGA	3 cut(s)	948, 1102, 1220
AsuII	TTCGAA	1 cut(s)	1261
AvaI	CYCGRG	1 cut(s)	1803
AvaII	GGWCC	3 cut(s)	1383, 1426, 1517
AxyI	CCTNAGG	1 cut(s)	591
BaeGI	GKGCMC	1 cut(s)	1417
BalI	TGGCCA	2 cut(s)	1245, 1587
BanI	GGYRCC	1 cut(s)	1993
BbsI	GAAGAC	3 cut(s)	491, 540, 1307
Bbv12I	GWGCWC	1 cut(s)	828
BbvI	GCAGC	7 cut(s)	123, 677, 680, 1004, 1178, 1318, 1993
BccI	CCATC	8 cut(s)	125, 407, 469, 474, 807, 1283, 1538, 2006
BciT130I	CCWGG	7 cut(s)	162, 546, 1022, 1721, 1747, 1834, 2334
BclI	TGATCA	2 cut(s)	868, 1738
BcoDI	GTCTC	1 cut(s)	1814
BcuI	ACTAGT	1 cut(s)	32
BfaI	CTAG	6 cut(s)	33, 855, 1217, 1538, 1923, 2063
BfmI	CTRYAG	2 cut(s)	1332, 1470
BfoI	RGCGCY	1 cut(s)	117
BglII	AGATCT	2 cut(s)	1249, 1855
BisI	GCNGC	7 cut(s)	112, 666, 669, 1018, 1192, 1332, 2007
BlsI	GCNGC	7 cut(s)	113, 667, 670, 1019, 1193, 1333, 2008
BmcAI	AGTACT	1 cut(s)	31
Bme1390I	CCNGG	7 cut(s)	162, 546, 1022, 1721, 1747, 1834, 2334
Bme18I	GGWCC	3 cut(s)	1383, 1426, 1517
BmeT110I	CYCGRG	1 cut(s)	1803
BmgT120I	GGNCC	5 cut(s)	635, 837, 1383, 1426, 1517
BmiI	GGNNCC	2 cut(s)	576, 1995
BmrFI	CCNGG	7 cut(s)	162, 546, 1022, 1721, 1747, 1834, 2334
BmrI	ACTGGG	1 cut(s)	203
BmsI	GCATC	3 cut(s)	230, 1228, 2232
BmuI	ACTGGG	1 cut(s)	203
BoxI	GACNNNNGTC	1 cut(s)	540
BpiI	GAAGAC	3 cut(s)	491, 540, 1307
BpmI	CTGGAG	1 cut(s)	1971
Bpu10I	CCTNAGC	2 cut(s)	1013, 1838
Bpu14I	TTCGAA	1 cut(s)	1261
Bsa29I	ATCGAT	1 cut(s)	238
BsaAI	YACGTR	1 cut(s)	1369
BsaHI	GRCGYC	1 cut(s)	2279
BsaI	GGTCTC	1 cut(s)	1814
BsaJI	CCNNGG	5 cut(s)	117, 545, 638, 1588, 1746
BsaWI	WCCGGW	3 cut(s)	263, 1234, 1934
Bsc4I	CCNNNNNNNGG	5 cut(s)	545, 644, 1241, 1839, 2375
Bse1I	ACTGG	3 cut(s)	209, 920, 986
Bse21I	CCTNAGG	1 cut(s)	591
BseAI	TCCGGA	1 cut(s)	1234
BseBI	CCWGG	7 cut(s)	162, 546, 1022, 1721, 1747, 1834, 2334
BseCI	ATCGAT	1 cut(s)	238
BseDI	CCNNGG	5 cut(s)	117, 545, 638, 1588, 1746
BseLI	CCNNNNNNNGG	5 cut(s)	545, 644, 1241, 1839, 2375
BseMII	CTCAG	2 cut(s)	582, 2250
BseNI	ACTGG	3 cut(s)	209, 920, 986
BseRI	GAGGAG	3 cut(s)	1694, 1802, 1953
BseSI	GKGCMC	1 cut(s)	1417
BseX3I	CGGCCG	1 cut(s)	2267
BseXI	GCAGC	7 cut(s)	123, 677, 680, 1004, 1178, 1318, 1993
Bsh1236I	CGCG	3 cut(s)	796, 1000, 2425
Bsh1285I	CGRYCG	1 cut(s)	2270
BshNI	GGYRCC	1 cut(s)	1993
BshVI	ATCGAT	1 cut(s)	238
BsiEI	CGRYCG	1 cut(s)	2270
BsiHKAI	GWGCWC	1 cut(s)	828
BsiHKCI	CYCGRG	1 cut(s)	1803
BsiSI	CCGG	3 cut(s)	264, 1235, 1935
BslFI	GGGAC	3 cut(s)	497, 1546, 1990
BslI	CCNNNNNNNGG	5 cut(s)	545, 644, 1241, 1839, 2375
BsmAI	GTCTC	1 cut(s)	1814
BsmFI	GGGAC	3 cut(s)	497, 1546, 1990
BsmI	GAATGC	1 cut(s)	1121
Bso31I	GGTCTC	1 cut(s)	1814
BsoBI	CYCGRG	1 cut(s)	1803
Bsp119I	TTCGAA	1 cut(s)	1261
Bsp1286I	GDGCHC	2 cut(s)	828, 1417
Bsp13I	TCCGGA	1 cut(s)	1234
Bsp1407I	TGTACA	1 cut(s)	735
Bsp143I	GATC	8 cut(s)	7, 868, 1210, 1249, 1738, 1855, 1942, 2200
Bsp19I	CCATGG	1 cut(s)	638
BspACI	CCGC	2 cut(s)	1439, 2425
BspCNI	CTCAG	2 cut(s)	583, 2251
BspDI	ATCGAT	1 cut(s)	238
BspEI	TCCGGA	1 cut(s)	1234
BspFNI	CGCG	3 cut(s)	796, 1000, 2425
BspLI	GGNNCC	2 cut(s)	576, 1995
BspMAI	CTGCAG	1 cut(s)	1336
BspT104I	TTCGAA	1 cut(s)	1261
BspT107I	GGYRCC	1 cut(s)	1993
BspTNI	GGTCTC	1 cut(s)	1814
BsrGI	TGTACA	1 cut(s)	735
BsrI	ACTGG	3 cut(s)	209, 920, 986
BssECI	CCNNGG	5 cut(s)	117, 545, 638, 1588, 1746
BssMI	GATC	8 cut(s)	7, 868, 1210, 1249, 1738, 1855, 1942, 2200
BssNI	GRCGYC	1 cut(s)	2279
BssT1I	CCWWGG	3 cut(s)	117, 638, 1588
Bst2UI	CCWGG	7 cut(s)	162, 546, 1022, 1721, 1747, 1834, 2334
Bst4CI	ACNGT	5 cut(s)	220, 322, 917, 1142, 1761
Bst6I	CTCTTC	2 cut(s)	1559, 1958
BstACI	GRCGYC	1 cut(s)	2279
BstAUI	TGTACA	1 cut(s)	735
BstBAI	YACGTR	1 cut(s)	1369
BstBI	TTCGAA	1 cut(s)	1261
BstC8I	GCNNGC	2 cut(s)	1243, 2365
BstDEI	CTNAG	7 cut(s)	345, 591, 650, 674, 1013, 1838, 2259
BstDSI	CCRYGG	1 cut(s)	638
BstENI	CCTNNNNNAGG	1 cut(s)	2373
BstFNI	CGCG	3 cut(s)	796, 1000, 2425
BstH2I	RGCGCY	1 cut(s)	117
BstHHI	GCGC	6 cut(s)	116, 665, 798, 1002, 1196, 2425
BstKTI	GATC	8 cut(s)	10, 871, 1213, 1252, 1741, 1858, 1945, 2203
BstMAI	GTCTC	1 cut(s)	1814
BstMBI	GATC	8 cut(s)	7, 868, 1210, 1249, 1738, 1855, 1942, 2200
BstMCI	CGRYCG	1 cut(s)	2270
BstMWI	GCNNNNNNNGC	2 cut(s)	350, 1584
BstNI	CCWGG	7 cut(s)	162, 546, 1022, 1721, 1747, 1834, 2334
BstNSI	RCATGY	2 cut(s)	736, 1708
BstPAI	GACNNNNGTC	1 cut(s)	540
BstSCI	CCNGG	7 cut(s)	160, 544, 1020, 1719, 1745, 1832, 2332
BstSFI	CTRYAG	2 cut(s)	1332, 1470
BstSLI	GKGCMC	1 cut(s)	1417
BstUI	CGCG	3 cut(s)	796, 1000, 2425
BstV1I	GCAGC	7 cut(s)	123, 677, 680, 1004, 1178, 1318, 1993
BstV2I	GAAGAC	3 cut(s)	491, 540, 1307
BstX2I	RGATCY	2 cut(s)	1249, 1855
BstXI	CCANNNNNNTGG	1 cut(s)	966
BstYI	RGATCY	2 cut(s)	1249, 1855
BstZI	CGGCCG	1 cut(s)	2267
Bsu15I	ATCGAT	1 cut(s)	238
Bsu36I	CCTNAGG	1 cut(s)	591
BsuTUI	ATCGAT	1 cut(s)	238
BtgI	CCRYGG	1 cut(s)	638
BtgZI	GCGATG	2 cut(s)	161, 785
BtsI	GCAGTG	1 cut(s)	1341
BtsIMutI	CAGTG	4 cut(s)	106, 664, 1053, 1341
Cac8I	GCNNGC	2 cut(s)	1243, 2365
CfoI	GCGC	6 cut(s)	116, 665, 798, 1002, 1196, 2425
Cfr13I	GGNCC	5 cut(s)	635, 837, 1383, 1426, 1517
ClaI	ATCGAT	1 cut(s)	238
CseI	GACGC	1 cut(s)	2268
CsiI	ACCWGGT	1 cut(s)	2332
CspCI	CAANNNNNGTGG	2 cut(s)	1772, 1807
DdeI	CTNAG	7 cut(s)	345, 591, 650, 674, 1013, 1838, 2259
DpnI	GATC	8 cut(s)	9, 870, 1212, 1251, 1740, 1857, 1944, 2202
DpnII	GATC	8 cut(s)	7, 868, 1210, 1249, 1738, 1855, 1942, 2200
DrdI	GACNNNNNNGTC	2 cut(s)	982, 1043
DseDI	GACNNNNNNGTC	2 cut(s)	982, 1043
EaeI	YGGCCR	3 cut(s)	1243, 1585, 2267
EagI	CGGCCG	1 cut(s)	2267
Eam1104I	CTCTTC	2 cut(s)	1559, 1958
EarI	CTCTTC	2 cut(s)	1559, 1958
EclXI	CGGCCG	1 cut(s)	2267
Eco130I	CCWWGG	3 cut(s)	117, 638, 1588
Eco147I	AGGCCT	2 cut(s)	679, 2161
Eco31I	GGTCTC	1 cut(s)	1814
Eco32I	GATATC	2 cut(s)	293, 1222
Eco47I	GGWCC	3 cut(s)	1383, 1426, 1517
Eco52I	CGGCCG	1 cut(s)	2267
Eco57I	CTGAAG	5 cut(s)	139, 485, 1257, 1451, 1619
Eco81I	CCTNAGG	1 cut(s)	591
Eco88I	CYCGRG	1 cut(s)	1803
EcoNI	CCTNNNNNAGG	1 cut(s)	2373
EcoO109I	RGGNCCY	2 cut(s)	837, 1426
EcoRI	GAATTC	1 cut(s)	551
EcoRII	CCWGG	7 cut(s)	160, 544, 1020, 1719, 1745, 1832, 2332
EcoRV	GATATC	2 cut(s)	293, 1222
EcoT14I	CCWWGG	3 cut(s)	117, 638, 1588
EcoT22I	ATGCAT	1 cut(s)	586
ErhI	CCWWGG	3 cut(s)	117, 638, 1588
FalI	AAGNNNNNCTT	2 cut(s)	2220, 2252
FaqI	GGGAC	3 cut(s)	497, 1546, 1990
FauI	CCCGC	1 cut(s)	1446
FauNDI	CATATG	1 cut(s)	1981
FbaI	TGATCA	2 cut(s)	868, 1738
FblI	GTMKAC	1 cut(s)	934
Fnu4HI	GCNGC	7 cut(s)	112, 666, 669, 1018, 1192, 1332, 2007
Fsp4HI	GCNGC	7 cut(s)	112, 666, 669, 1018, 1192, 1332, 2007
FspBI	CTAG	6 cut(s)	33, 855, 1217, 1538, 1923, 2063
GlaI	GCGC	6 cut(s)	115, 664, 797, 1001, 1195, 2424
GluI	GCNGC	7 cut(s)	112, 666, 669, 1018, 1192, 1332, 2007
GsuI	CTGGAG	1 cut(s)	1971
HaeII	RGCGCY	1 cut(s)	117
HapII	CCGG	3 cut(s)	264, 1235, 1935
HgaI	GACGC	1 cut(s)	2268
HhaI	GCGC	6 cut(s)	116, 665, 798, 1002, 1196, 2425
Hin1I	GRCGYC	1 cut(s)	2279
Hin6I	GCGC	6 cut(s)	114, 663, 796, 1000, 1194, 2423
HinP1I	GCGC	6 cut(s)	114, 663, 796, 1000, 1194, 2423
HincII	GTYRAC	1 cut(s)	73
HindII	GTYRAC	1 cut(s)	73
HindIII	AAGCTT	2 cut(s)	433, 1496
HinfI	GANTC	9 cut(s)	257, 380, 654, 942, 1231, 1657, 1894, 2177, 2263
HpaII	CCGG	3 cut(s)	264, 1235, 1935
HphI	GGTGA	3 cut(s)	948, 1102, 1220
Hpy166II	GTNNAC	9 cut(s)	54, 73, 333, 727, 935, 1093, 1341, 1372, 1525
Hpy188III	TCNNGA	7 cut(s)	384, 1235, 1950, 1975, 2105, 2258, 2345
Hpy8I	GTNNAC	9 cut(s)	54, 73, 333, 727, 935, 1093, 1341, 1372, 1525
Hpy99I	CGWCG	2 cut(s)	91, 790
HpyAV	CCTTC	7 cut(s)	456, 1403, 1499, 2073, 2147, 2151, 2351
HpyCH4III	ACNGT	5 cut(s)	220, 322, 917, 1142, 1761
HpyCH4IV	ACGT	3 cut(s)	1368, 1777, 2218
HpyF10VI	GCNNNNNNNGC	2 cut(s)	350, 1584
HpyF3I	CTNAG	7 cut(s)	345, 591, 650, 674, 1013, 1838, 2259
HpySE526I	ACGT	3 cut(s)	1368, 1777, 2218
Hsp92I	GRCGYC	1 cut(s)	2279
HspAI	GCGC	6 cut(s)	114, 663, 796, 1000, 1194, 2423
Kpn2I	TCCGGA	1 cut(s)	1234
Ksp22I	TGATCA	2 cut(s)	868, 1738
Kzo9I	GATC	8 cut(s)	7, 868, 1210, 1249, 1738, 1855, 1942, 2200
LmnI	GCTCC	2 cut(s)	887, 1440
Lsp1109I	GCAGC	7 cut(s)	123, 677, 680, 1004, 1178, 1318, 1993
LweI	GCATC	3 cut(s)	230, 1228, 2232
MabI	ACCWGGT	1 cut(s)	2332
MaeI	CTAG	6 cut(s)	33, 855, 1217, 1538, 1923, 2063
MaeII	ACGT	3 cut(s)	1368, 1777, 2218
MaeIII	GTNAC	7 cut(s)	164, 406, 459, 911, 954, 984, 2214
MalI	GATC	8 cut(s)	9, 870, 1212, 1251, 1740, 1857, 1944, 2202
MboI	GATC	8 cut(s)	7, 868, 1210, 1249, 1738, 1855, 1942, 2200
MflI	RGATCY	2 cut(s)	1249, 1855
MhlI	GDGCHC	2 cut(s)	828, 1417
MlsI	TGGCCA	2 cut(s)	1245, 1587
MluNI	TGGCCA	2 cut(s)	1245, 1587
MlyI	GAGTC	1 cut(s)	251
MmeI	TCCRAC	1 cut(s)	1734
Mox20I	TGGCCA	2 cut(s)	1245, 1587
Mph1103I	ATGCAT	1 cut(s)	586
MroI	TCCGGA	1 cut(s)	1234
MscI	TGGCCA	2 cut(s)	1245, 1587
MseI	TTAA	8 cut(s)	213, 776, 1173, 1355, 1391, 1596, 1931, 2186
MslI	CAYNNNNRTG	3 cut(s)	714, 991, 1262
Msp20I	TGGCCA	2 cut(s)	1245, 1587
MspI	CCGG	3 cut(s)	264, 1235, 1935
MspR9I	CCNGG	7 cut(s)	162, 546, 1022, 1721, 1747, 1834, 2334
Mva1269I	GAATGC	1 cut(s)	1121
MvaI	CCWGG	7 cut(s)	162, 546, 1022, 1721, 1747, 1834, 2334
MvnI	CGCG	3 cut(s)	796, 1000, 2425
MwoI	GCNNNNNNNGC	2 cut(s)	350, 1584
NcoI	CCATGG	1 cut(s)	638
NdeI	CATATG	1 cut(s)	1981
NdeII	GATC	8 cut(s)	7, 868, 1210, 1249, 1738, 1855, 1942, 2200
NlaIV	GGNNCC	2 cut(s)	576, 1995
NmeAIII	GCCGAG	1 cut(s)	2295
NmuCI	GTSAC	3 cut(s)	406, 954, 984
NsiI	ATGCAT	1 cut(s)	586
NspI	RCATGY	2 cut(s)	736, 1708
NspV	TTCGAA	1 cut(s)	1261
PceI	AGGCCT	2 cut(s)	679, 2161
PciI	ACATGT	2 cut(s)	732, 1704
PctI	GAATGC	1 cut(s)	1121
PfeI	GAWTC	8 cut(s)	380, 654, 942, 1231, 1657, 1894, 2177, 2263
PflMI	CCANNNNNTGG	1 cut(s)	644
PkrI	GCNGC	7 cut(s)	113, 667, 670, 1019, 1193, 1333, 2008
PleI	GAGTC	1 cut(s)	251
PpsI	GAGTC	1 cut(s)	251
Ppu21I	YACGTR	1 cut(s)	1369
PpuMI	RGGWCCY	1 cut(s)	1426
PscI	ACATGT	2 cut(s)	732, 1704
PshAI	GACNNNNGTC	1 cut(s)	540
PsiI	TTATAA	3 cut(s)	1619, 2072, 2291
Psp1406I	AACGTT	1 cut(s)	1777
Psp5II	RGGWCCY	1 cut(s)	1426
Psp6I	CCWGG	7 cut(s)	160, 544, 1020, 1719, 1745, 1832, 2332
PspGI	CCWGG	7 cut(s)	160, 544, 1020, 1719, 1745, 1832, 2332
PspN4I	GGNNCC	2 cut(s)	576, 1995
PspPI	GGNCC	5 cut(s)	635, 837, 1383, 1426, 1517
PspPPI	RGGWCCY	1 cut(s)	1426
PsrI	GAACNNNNNNTAC	2 cut(s)	775, 807
PstI	CTGCAG	1 cut(s)	1336
PsuI	RGATCY	2 cut(s)	1249, 1855
RseI	CAYNNNNRTG	3 cut(s)	714, 991, 1262
SaqAI	TTAA	8 cut(s)	213, 776, 1173, 1355, 1391, 1596, 1931, 2186
SatI	GCNGC	7 cut(s)	112, 666, 669, 1018, 1192, 1332, 2007
Sau3AI	GATC	8 cut(s)	7, 868, 1210, 1249, 1738, 1855, 1942, 2200
Sau96I	GGNCC	5 cut(s)	635, 837, 1383, 1426, 1517
ScaI	AGTACT	1 cut(s)	31
SchI	GAGTC	1 cut(s)	251
ScrFI	CCNGG	7 cut(s)	162, 546, 1022, 1721, 1747, 1834, 2334
SduI	GDGCHC	2 cut(s)	828, 1417
SexAI	ACCWGGT	1 cut(s)	2332
SfaNI	GCATC	3 cut(s)	230, 1228, 2232
SfcI	CTRYAG	2 cut(s)	1332, 1470
SfuI	TTCGAA	1 cut(s)	1261
SinI	GGWCC	3 cut(s)	1383, 1426, 1517
SmiMI	CAYNNNNRTG	3 cut(s)	714, 991, 1262
SpeI	ACTAGT	1 cut(s)	32
SseBI	AGGCCT	2 cut(s)	679, 2161
SsiI	CCGC	2 cut(s)	1439, 2425
SspI	AATATT	1 cut(s)	1929
SspMI	CTAG	6 cut(s)	33, 855, 1217, 1538, 1923, 2063
StuI	AGGCCT	2 cut(s)	679, 2161
StyD4I	CCNGG	7 cut(s)	160, 544, 1020, 1719, 1745, 1832, 2332
StyI	CCWWGG	3 cut(s)	117, 638, 1588
TaaI	ACNGT	5 cut(s)	220, 322, 917, 1142, 1761
TaiI	ACGT	3 cut(s)	1371, 1780, 2221
TaqI	TCGA	5 cut(s)	238, 438, 699, 1261, 2390
TatI	WGTACW	3 cut(s)	29, 359, 735
TfiI	GAWTC	8 cut(s)	380, 654, 942, 1231, 1657, 1894, 2177, 2263
Tru1I	TTAA	8 cut(s)	213, 776, 1173, 1355, 1391, 1596, 1931, 2186
Tru9I	TTAA	8 cut(s)	213, 776, 1173, 1355, 1391, 1596, 1931, 2186
TscAI	CASTG	4 cut(s)	106, 664, 1060, 1341
TseFI	GTSAC	3 cut(s)	406, 954, 984
TseI	GCWGC	7 cut(s)	111, 665, 668, 1017, 1191, 1331, 2006
Tsp45I	GTSAC	3 cut(s)	406, 954, 984
TspGWI	ACGGA	2 cut(s)	75, 1728
TspRI	CASTG	4 cut(s)	106, 664, 1060, 1341
Van91I	CCANNNNNTGG	1 cut(s)	644
VpaK11BI	GGWCC	3 cut(s)	1383, 1426, 1517
XagI	CCTNNNNNAGG	1 cut(s)	2373
XceI	RCATGY	2 cut(s)	736, 1708
XcmI	CCANNNNNNNNNTGG	4 cut(s)	1717, 1790, 2123, 2413
XmiI	GTMKAC	1 cut(s)	934
XspI	CTAG	6 cut(s)	33, 855, 1217, 1538, 1923, 2063
ZrmI	AGTACT	1 cut(s)	31
Zsp2I	ATGCAT	1 cut(s)	586

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.