Prupe.4G195900_v2.0.a1

G-type lectin S-receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0000207
Physical Location & Seq
Forward (+)
11956463 .. 11959998
3536 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G195900.1

Sequence Viewer

Length: 2445 bp
ATGGAGCTCCTCTGTTTCTTGGTTTTCAGTATCTGCTTACTCTTTTCTCCGAGCAGAATTTCATCAGCTGCTGATACCATTACTTCATCTCAGTCCCTCAGTGATGGCAAGTCATTGGTTTCTTCTCCGGGTGGAATATTTGAGCTGGGTTTTTTCAGTCCAGGCATTTCCAAGAGCCGCTACTTGGGAATTTGGTACAAGAACATCCCAACTCCAACTGTTGTTTGGGTTGCAAACAGGCAAACTCCAATCAATGGTTCCACTGGCAGCTTGATGATCAACAGCACAGGCAATCTTGTCCTTGTCAACAACAACAACCAGACTGTTTGGTCTACAAATTCATCAAAACAGTCCCAGAGCCAGCGCCCTGTCCTGCAGCTCCTGGACAATGGCAATCTTGTCCTCAGAGATGAGAAAGATGCAAACTCAGAAAAGTACTTGTGGCAGAGTTTTGACTACCCATCTGATACTTTGTTGCCAGGAATGAAGCTTGGATGGGACTTGAGAACCGGACTTAATCGACGTTTAACGGCATGGAAAAGTCCAGATGACCCTTCTCCAGGGGACTTCATCTGGGAAATGGAGCTGCATGAGTATCCTGAGCCAGTTATGTGGAAAGGGTCCAAAGAGTACTTGAGAAGTGGCCCATGGAATGGTGTTTTGTTCAGTGGTAAACCAGCCAAGGCACTTCCTGCATTGAACTTCAGTTTCATTTCCAATGAGGATGAGGTTTACTTGACAATCGACATGGTGAATAAGTCTGTACTTGGAAGGATGATCATGAACCAAACCACGTCGACGCCGTATCGACAGGAGTGGATTTGGTCCGAAGCAGACCGGAATTGGACAATTTATGCAACGTTTCCAAGAGACCCTTGTGATAGTTATGGCCACTGTGGTGGTAATGGCAATTGTGATCTTAGTTCATCCCCAATTTGTCAATGTTTGGATAGGTTCAGTCCCAGATCACCGGAGAACTGGAACTTAAATGAGTTTTCACAAGGCTGTGAGCGCAGGAAGCCATTGAGCTGCAAGAATGATGGATTCGCCACGTATCCCGGGTTGAAATTGCCCGATACTACGCATAGTTGGGTAGATAAAAGCATGAATCTCAAGGAATGCAAGGCCAAATGCTTATCTAATTGTTCTTGTTCTGCTTATACAAACTTGGATGTGAGAGGAGGAGGCAGTGGTTGTGCCATCTGGTTCGATGATCTAGTCGATATCAAGCAAATGCCAGGTGGTGACCAGGATATATATATCAAGATATCTGCTTCAGAACTAGGAGGGAAAGATGAGAAGTGGAAGATAGCTGTGATTATTGCATCTGCCTCTGCCCTTATTCTTGGGATGCTCTTAGTTGGCTATTGTTGCATTCTCAAAGCCAGAGGCAGGAAATATGTCAAAGGTCAAGACATTGAAGAACAGAAAGACGAAGATCTGGAGCTGCCATTGTTCGACTTGACAACAATAGAGGGTGCCACTAATTTCTTTTCAGTCAACAATAAGCTTGGTGAAGGCGGTTTTGGACCTGTATACAAGGGTAGACTCGTAGATGGTCAAGAAATTGCAGTCAAGAGACTTTCAAGAAGTTCTGGACAAGGAATAAAGGAGTTCAAAAATGAAGTAATACTGATTGCCAAACTTCAACACCGAAATCTTGTAAAGCTTCTTGGTTGCTGCATTCAAGGAGAGGAGAAATTGCTGATCTATGAATACATGCCCAACAAAAGCTTGGACTACTTCCTTTTTGATGAAACACGACGTAAATTGTTAGATTGGCCTCAGCGCTTCCAAATAATCTGTGGGATTGCTAGGGGGCTTCTCTATCTTCATCAGGATTCCAGACTGAGGATTATACACAGAGATCTCAAAGCAAGTAATGTTTTACTTGATAGAGACTTGAACCCGAAGATCTCAGATTTTGGTCTTGCTAGAACTTTTGGTGGAGATCAAACTGAAGGAAATACAAACAGAGTGGTTGGAACATATGGTTACATGGCACCTGAATATGCTACTGAAGGGCAATTCTCAGTGAAATCTGATGTCTTTAGCTTTGGTATTTTACTGCTGGAGATAATAAGTGGAAAGAAAAGTAAAGGGTTCTATCATTTGAACCACCGCCTGAATCTTATTGGAAATGCATGGAGATTGTGGAAAGAAGGAAGGCCATTAGAGCTGATTGACGAAGGGTTTGGGAACTCGTGCACTCTATCAGACGTACTGCGATGCATCCATGTCAGTCTGTTATGTGTGCAACTACAGCCTGAGGACAGGCCAACCATGTCATCTGTAGTTCAGATGTTATGTAGTGAAAGTGCCTTGCCTGAGCCCAAAGAACCAGGATTTGTTCCAGAAAAGGATTTACTCAACGGAGAGTTTCCCCTAATTAACAACGAACCATCTTCTAATGGTTTAACCATTACACAACTCGAGCCTCGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase_Tyr S_locus_glycop

Protein Analysis

815

Amino Acids

91.15

Weight (kDa)

6.01

Isoelectric Point (pI)

43.06

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000207)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G27300
fragaria_vesca FvH4_3g15930 FvH4_3g15980 FvH4_3g15980 FvH4_3g15980 FvH4_3g15980 FvH4_3g21350 FvH4_3g21400 FvH4_3g21400 FvH4_6g12332
malus_domestica MD02G1167100.v1.1 MD02G1167200.v1.1 MD03G1185600.v1.1 MD05G1213900.v1.1 MD05G1214100.v1.1 MD05G1214200.v1.1 MD05G1214700.v1.1 MD05G1216300.v1.1 MD05G1216800.v1.1 MD05G1217300.v1.1 MD05G1218000.v1.1 MD11G1231100.v1.1 MD11G1231200.v1.1 MD11G1231400.v1.1 MD11G1231500.v1.1 MD11G1232400.v1.1 MD11G1232500.v1.1
prunus_persica Prupe.4G142300_v2.0.a1 Prupe.4G142400_v2.0.a1 Prupe.4G142500_v2.0.a1 Prupe.4G142600_v2.0.a1 Prupe.4G142800_v2.0.a1 Prupe.4G195000_v2.0.a1 Prupe.4G195000_v2.0.a1 Prupe.4G195000_v2.0.a1 Prupe.4G195100_v2.0.a1 Prupe.4G195300_v2.0.a1 Prupe.4G195300_v2.0.a1 Prupe.4G195300_v2.0.a1 Prupe.4G195400_v2.0.a1 Prupe.4G195400_v2.0.a1 Prupe.4G195500_v2.0.a1 Prupe.4G195700_v2.0.a1 Prupe.4G195900_v2.0.a1
pyrus_communis pycom03g16540 pycom05g19920 pycom11g20460 pycom11g20480 pycom11g20490 pycom11g20500 pycom11g20530
rosa_chinensis RchiOBHm_Chr2g0139311 RchiOBHm_Chr2g0139351 RchiOBHm_Chr5g0026801 RchiOBHm_Chr5g0026851 RchiOBHm_Chr5g0026891 RchiOBHm_Chr5g0026911 RchiOBHm_Chr5g0036571 RchiOBHm_Chr5g0036591 RchiOBHm_Chr5g0036721 RchiOBHm_Chr5g0036741 RchiOBHm_Chr7g0222981
rosa_laevigata RLG00000002006 RLG00000019807 RLG00000032964 RLG00000032968 RLG00000032969 RLG00000033706 RLG00000033708 RLG00000033712 RLG00000033716
rosa_multiflora Rmu_co8256651.1_g000001 Rmu_co8279377.1_g000001 Rmu_co8429919.1_g000001 Rmu_co8490103.1_g000001 Rmu_sc0000774.1_g000002 Rmu_sc0002525.1_g000012 Rmu_sc0004223.1_g000008 Rmu_sc0004390.1_g000002 Rmu_sc0006059.1_g000014 Rmu_sc0006059.1_g000024 Rmu_sc0006514.1_g000020 Rmu_sc0014333.1_g000002 Rmu_ssc0000172.1_g000018 Rmu_ssc0000172.1_g000029
rosa_roxburghii Rroxscaffold_1G00044230 Rroxscaffold_1G00044260 Rroxscaffold_1G00044320 Rroxscaffold_1G00044340 Rroxscaffold_1G00052560 Rroxscaffold_1G00052570 Rroxscaffold_1G00052660 Rroxscaffold_1G00052720 Rroxscaffold_2G00105450 Rroxscaffold_2G00105460
rosa_rugosa Rorug02G0356900 Rorug02G0357000 Rorug02G0357100 Rorug02G0357200 Rorug02G0357300 Rorug02G0357400 Rorug02G0357500 Rorug02G0357600 Rorug05G0095400 Rorug05G0095500 Rorug05G0095500 Rorug05G0095600 Rorug05G0095700 Rorug05G0095800 Rorug05G0157700 Rorug05G0157800
rosa_samantha Rh2AG406800 Rh2AG406900 Rh2BG417300 Rh2CG392900 Rh2DG426200 Rh5AG189300 Rh5AG249400 Rh5AG250100 Rh5BG186300 Rh5BG186900 Rh5BG187000 Rh5BG249500 Rh5BG250600 Rh5BG250900 Rh5BG251500 Rh5BG251800 Rh5CG205500 Rh5CG206300 Rh5CG206700 Rh5CG283000 Rh5CG283300 Rh5CG283500 Rh5DG188500 Rh5DG188700 Rh5DG258700 Rh5DG258900 Rh5DG259600 Rh5DG259900 Rh7CG375800 Rh7DG352500
rosa_wichuraiana Rw2G032960 Rw2G032970 Rw2G033220 Rw2G033230 Rw5G017130 Rw5G017230 Rw5G022940 Rw5G022960 Rw5G022980 Rw7G030420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 328
AccB1I GGYRCC 2 cut(s) 1478, 2002
AccB7I CCANNNNNTGG 2 cut(s) 254, 653
AccI GTMKAC 4 cut(s) 332, 797, 1536, 1546
AciI CCGC 3 cut(s) 178, 1521, 2122
AclI AACGTT 1 cut(s) 860
AcoI YGGCCR 1 cut(s) 889
AcsI RAATTY 3 cut(s) 57, 189, 337
AcuI CTGAAG 4 cut(s) 688, 1260, 1980, 2040
AcyI GRCGYC 1 cut(s) 800
AfaI GTAC 5 cut(s) 197, 437, 632, 765, 2223
AfeI AGCGCT 1 cut(s) 1790
AfiI CCNNNNNNNGG 6 cut(s) 184, 254, 560, 653, 1392, 1851
AgsI TTSAA 9 cut(s) 700, 1066, 1421, 1587, 1618, 1649, 1688, 1906, 2116
AjiI CACGTC 1 cut(s) 795
AjnI CCWGG 7 cut(s) 160, 381, 478, 559, 1237, 1248, 2341
AjuI GAANNNNNNNTTGG 4 cut(s) 241, 273, 1509, 1541
AleI CACNNNNGTG 1 cut(s) 897
Alw21I GWGCWC 2 cut(s) 9, 2210
Alw26I GTCTC 3 cut(s) 864, 1573, 1893
Alw44I GTGCAC 1 cut(s) 2206
AlwNI CAGNNNCTG 3 cut(s) 33, 71, 382
Ama87I CYCGRG 2 cut(s) 1058, 2432
Aor51HI AGCGCT 1 cut(s) 1790
AoxI GGCC 6 cut(s) 643, 889, 1125, 1781, 2168, 2276
ApaLI GTGCAC 1 cut(s) 2206
ApeKI GCWGC 7 cut(s) 68, 267, 376, 586, 1029, 1447, 1680
ApoI RAATTY 3 cut(s) 57, 189, 337
ArsI GACNNNNNNTTYG 2 cut(s) 2177, 2209
AspLEI GCGC 3 cut(s) 366, 1014, 1791
AspS9I GGNCC 4 cut(s) 621, 644, 825, 1529
AsuC2I CCSGG 3 cut(s) 129, 1059, 1060
AsuHPI GGTGA 4 cut(s) 763, 960, 1256, 1526
AvaI CYCGRG 2 cut(s) 1058, 2432
AvaII GGWCC 3 cut(s) 621, 825, 1529
AxyI CCTNAGG 1 cut(s) 2268
BaeGI GKGCMC 1 cut(s) 2210
BalI TGGCCA 1 cut(s) 891
BanI GGYRCC 2 cut(s) 1478, 2002
BanII GRGCYC 2 cut(s) 9, 2334
BauI CACGAG 1 cut(s) 2203
Bbv12I GWGCWC 2 cut(s) 9, 2210
BbvCI CCTCAGC 1 cut(s) 1785
BbvI GCAGC 7 cut(s) 55, 279, 388, 573, 1016, 1434, 1667
BccI CCATC 7 cut(s) 98, 469, 489, 1034, 1208, 1550, 2410
BceAI ACGGC 2 cut(s) 546, 787
BciT130I CCWGG 7 cut(s) 162, 383, 480, 561, 1239, 1250, 2343
BciVI GTATCC 2 cut(s) 606, 1065
BclI TGATCA 2 cut(s) 276, 777
BcnI CCSGG 3 cut(s) 129, 1059, 1060
BcoDI GTCTC 3 cut(s) 864, 1573, 1893
BfaI CTAG 4 cut(s) 1217, 1283, 1815, 1935
BfmI CTRYAG 3 cut(s) 374, 2261, 2292
BfoI RGCGCY 2 cut(s) 367, 1792
BfuI GTATCC 2 cut(s) 606, 1065
BglII AGATCT 3 cut(s) 1438, 1867, 1914
BisI GCNGC 8 cut(s) 69, 178, 268, 377, 587, 1030, 1448, 1681
BlsI GCNGC 8 cut(s) 70, 179, 269, 378, 588, 1031, 1449, 1682
BmcAI AGTACT 2 cut(s) 437, 632
Bme18I GGWCC 3 cut(s) 621, 825, 1529
BmeT110I CYCGRG 2 cut(s) 1058, 2432
BmgBI CACGTC 1 cut(s) 795
BmgT120I GGNCC 4 cut(s) 621, 644, 825, 1529
BmiI GGNNCC 4 cut(s) 259, 622, 1480, 2004
BmsI GCATC 5 cut(s) 409, 1334, 1341, 2219, 2241
BpmI CTGGAG 3 cut(s) 543, 1463, 2093
Bpu10I CCTNAGC 3 cut(s) 600, 1785, 2328
BpuEI CTTGAG 3 cut(s) 523, 655, 1097
BpuMI CCSGG 3 cut(s) 129, 1059, 1060
BsaAI YACGTR 1 cut(s) 1053
BsaHI GRCGYC 1 cut(s) 800
BsaI GGTCTC 1 cut(s) 864
BsaJI CCNNGG 4 cut(s) 560, 647, 681, 1058
BsaWI WCCGGW 3 cut(s) 509, 837, 970
Bsc4I CCNNNNNNNGG 6 cut(s) 184, 254, 560, 653, 1392, 1851
Bse1I ACTGG 3 cut(s) 268, 605, 983
Bse21I CCTNAGG 1 cut(s) 2268
BseBI CCWGG 7 cut(s) 162, 383, 480, 561, 1239, 1250, 2343
BseDI CCNNGG 4 cut(s) 560, 647, 681, 1058
BseGI GGATG 8 cut(s) 204, 500, 730, 780, 926, 1177, 1356, 2232
BseLI CCNNNNNNNGG 6 cut(s) 184, 254, 560, 653, 1392, 1851
BseNI ACTGG 3 cut(s) 268, 605, 983
BseRI GAGGAG 3 cut(s) 1194, 1197, 1709
BseSI GKGCMC 1 cut(s) 2210
BseXI GCAGC 7 cut(s) 55, 279, 388, 573, 1016, 1434, 1667
BseYI CCCAGC 1 cut(s) 145
BshFI GGCC 6 cut(s) 645, 891, 1127, 1783, 2170, 2278
BshNI GGYRCC 2 cut(s) 1478, 2002
BsiHKAI GWGCWC 2 cut(s) 9, 2210
BsiHKCI CYCGRG 2 cut(s) 1058, 2432
BsiSI CCGG 5 cut(s) 128, 510, 838, 971, 1059
BslFI GGGAC 5 cut(s) 79, 337, 512, 578, 945
BslI CCNNNNNNNGG 6 cut(s) 184, 254, 560, 653, 1392, 1851
BsmAI GTCTC 3 cut(s) 864, 1573, 1893
BsmFI GGGAC 5 cut(s) 79, 337, 512, 578, 945
BsmI GAATGC 3 cut(s) 1124, 1374, 1683
BsnI GGCC 6 cut(s) 645, 891, 1127, 1783, 2170, 2278
Bso31I GGTCTC 1 cut(s) 864
BsoBI CYCGRG 2 cut(s) 1058, 2432
Bsp1286I GDGCHC 3 cut(s) 9, 2210, 2334
Bsp19I CCATGG 1 cut(s) 647
BspACI CCGC 3 cut(s) 178, 1521, 2122
BspANI GGCC 6 cut(s) 645, 891, 1127, 1783, 2170, 2278
BspHI TCATGA 1 cut(s) 780
BspLI GGNNCC 4 cut(s) 259, 622, 1480, 2004
BspMAI CTGCAG 1 cut(s) 378
BspT107I GGYRCC 2 cut(s) 1478, 2002
BspTNI GGTCTC 1 cut(s) 864
BsrI ACTGG 3 cut(s) 268, 605, 983
BssECI CCNNGG 4 cut(s) 560, 647, 681, 1058
BssNAI GTATAC 1 cut(s) 1537
BssNI GRCGYC 1 cut(s) 800
BssSI CACGAG 1 cut(s) 2203
BssT1I CCWWGG 2 cut(s) 647, 681
Bst1107I GTATAC 1 cut(s) 1537
Bst2BI CACGAG 1 cut(s) 2203
Bst2UI CCWGG 7 cut(s) 162, 383, 480, 561, 1239, 1250, 2343
Bst4CI ACNGT 4 cut(s) 220, 325, 351, 896
BstACI GRCGYC 1 cut(s) 800
BstAPI GCANNNNNTGC 1 cut(s) 692
BstBAI YACGTR 1 cut(s) 1053
BstC8I GCNNGC 1 cut(s) 362
BstDSI CCRYGG 1 cut(s) 647
BstEII GGTNACC 1 cut(s) 1244
BstENI CCTNNNNNAGG 1 cut(s) 558
BstF5I GGATG 8 cut(s) 204, 500, 730, 780, 926, 1177, 1356, 2232
BstH2I RGCGCY 2 cut(s) 367, 1792
BstHHI GCGC 3 cut(s) 366, 1014, 1791
BstMAI GTCTC 3 cut(s) 864, 1573, 1893
BstMWI GCNNNNNNNGC 6 cut(s) 692, 1011, 1018, 1371, 2176, 2263
BstNI CCWGG 7 cut(s) 162, 383, 480, 561, 1239, 1250, 2343
BstNSI RCATGY 1 cut(s) 1723
BstPI GGTNACC 1 cut(s) 1244
BstSFI CTRYAG 3 cut(s) 374, 2261, 2292
BstSLI GKGCMC 1 cut(s) 2210
BstV1I GCAGC 7 cut(s) 55, 279, 388, 573, 1016, 1434, 1667
BstX2I RGATCY 3 cut(s) 1438, 1867, 1914
BstXI CCANNNNNNTGG 2 cut(s) 612, 899
BstYI RGATCY 3 cut(s) 1438, 1867, 1914
BstZ17I GTATAC 1 cut(s) 1537
Bsu36I CCTNAGG 1 cut(s) 2268
BsuI GTATCC 2 cut(s) 606, 1065
BsuRI GGCC 6 cut(s) 645, 891, 1127, 1783, 2170, 2278
BtgI CCRYGG 1 cut(s) 647
BtgZI GCGATG 1 cut(s) 2242
BtrI CACGTC 1 cut(s) 795
BtsCI GGATG 8 cut(s) 204, 500, 730, 780, 926, 1177, 1356, 2232
BtsI GCAGTG 1 cut(s) 1195
BtsIMutI CAGTG 6 cut(s) 106, 261, 673, 892, 1195, 2040
Cac8I GCNNGC 1 cut(s) 362
CaiI CAGNNNCTG 3 cut(s) 33, 71, 382
CciI TCATGA 1 cut(s) 780
CfoI GCGC 3 cut(s) 366, 1014, 1791
Cfr13I GGNCC 4 cut(s) 621, 644, 825, 1529
Cfr9I CCCGGG 1 cut(s) 1058
CseI GACGC 1 cut(s) 808
Csp6I GTAC 5 cut(s) 196, 436, 631, 764, 2222
CspCI CAANNNNNGTGG 2 cut(s) 1959, 1994
CviQI GTAC 5 cut(s) 196, 436, 631, 764, 2222
DrdI GACNNNNNNGTC 1 cut(s) 328
DseDI GACNNNNNNGTC 1 cut(s) 328
EaeI YGGCCR 1 cut(s) 889
Ecl136II GAGCTC 1 cut(s) 7
Eco130I CCWWGG 2 cut(s) 647, 681
Eco24I GRGCYC 2 cut(s) 9, 2334
Eco31I GGTCTC 1 cut(s) 864
Eco32I GATATC 2 cut(s) 1225, 1269
Eco47I GGWCC 3 cut(s) 621, 825, 1529
Eco47III AGCGCT 1 cut(s) 1790
Eco53kI GAGCTC 1 cut(s) 7
Eco57I CTGAAG 4 cut(s) 688, 1260, 1980, 2040
Eco81I CCTNAGG 1 cut(s) 2268
Eco88I CYCGRG 2 cut(s) 1058, 2432
Eco91I GGTNACC 1 cut(s) 1244
EcoICRI GAGCTC 1 cut(s) 7
EcoNI CCTNNNNNAGG 1 cut(s) 558
EcoO65I GGTNACC 1 cut(s) 1244
EcoRII CCWGG 7 cut(s) 160, 381, 478, 559, 1237, 1248, 2341
EcoRV GATATC 2 cut(s) 1225, 1269
EcoT14I CCWWGG 2 cut(s) 647, 681
EcoT22I ATGCAT 2 cut(s) 2146, 2234
EcoT38I GRGCYC 2 cut(s) 9, 2334
ErhI CCWWGG 2 cut(s) 647, 681
FalI AAGNNNNNCTT 2 cut(s) 859, 891
FaqI GGGAC 5 cut(s) 79, 337, 512, 578, 945
FauNDI CATATG 1 cut(s) 1990
FbaI TGATCA 2 cut(s) 276, 777
FblI GTMKAC 4 cut(s) 332, 797, 1536, 1546
Fnu4HI GCNGC 8 cut(s) 69, 178, 268, 377, 587, 1030, 1448, 1681
FokI GGATG 8 cut(s) 191, 507, 737, 787, 913, 1184, 1363, 2219
FriOI GRGCYC 2 cut(s) 9, 2334
Fsp4HI GCNGC 8 cut(s) 69, 178, 268, 377, 587, 1030, 1448, 1681
FspBI CTAG 4 cut(s) 1217, 1283, 1815, 1935
GlaI GCGC 3 cut(s) 365, 1013, 1790
GluI GCNGC 8 cut(s) 69, 178, 268, 377, 587, 1030, 1448, 1681
GsaI CCCAGC 1 cut(s) 149
GsuI CTGGAG 3 cut(s) 543, 1463, 2093
HaeII RGCGCY 2 cut(s) 367, 1792
HaeIII GGCC 6 cut(s) 645, 891, 1127, 1783, 2170, 2278
HapII CCGG 5 cut(s) 128, 510, 838, 971, 1059
HgaI GACGC 1 cut(s) 808
HhaI GCGC 3 cut(s) 366, 1014, 1791
Hin1I GRCGYC 1 cut(s) 800
Hin6I GCGC 3 cut(s) 364, 1012, 1789
HinP1I GCGC 3 cut(s) 364, 1012, 1789
HincII GTYRAC 3 cut(s) 307, 798, 1501
HindII GTYRAC 3 cut(s) 307, 798, 1501
HindIII AAGCTT 4 cut(s) 488, 1508, 1667, 1732
HinfI GANTC 5 cut(s) 1044, 1108, 1548, 1841, 2128
HpaII CCGG 5 cut(s) 128, 510, 838, 971, 1059
HphI GGTGA 4 cut(s) 763, 960, 1256, 1526
Hpy166II GTNNAC 9 cut(s) 307, 333, 674, 733, 798, 1501, 1537, 1547, 2208
Hpy8I GTNNAC 9 cut(s) 307, 333, 674, 733, 798, 1501, 1537, 1547, 2208
Hpy99I CGWCG 4 cut(s) 525, 799, 802, 1767
HpyAV CCTTC 8 cut(s) 564, 765, 1511, 1955, 2015, 2156, 2160, 2183
HpyCH4III ACNGT 4 cut(s) 220, 325, 351, 896
HpyCH4IV ACGT 6 cut(s) 523, 794, 860, 1052, 1765, 2220
HpyF10VI GCNNNNNNNGC 6 cut(s) 692, 1011, 1018, 1371, 2176, 2263
HpySE526I ACGT 6 cut(s) 523, 794, 860, 1052, 1765, 2220
Hsp92I GRCGYC 1 cut(s) 800
HspAI GCGC 3 cut(s) 364, 1012, 1789
Ksp22I TGATCA 2 cut(s) 276, 777
LmnI GCTCC 5 cut(s) 4, 12, 384, 583, 1444
Lsp1109I GCAGC 7 cut(s) 55, 279, 388, 573, 1016, 1434, 1667
LweI GCATC 5 cut(s) 409, 1334, 1341, 2219, 2241
MaeI CTAG 4 cut(s) 1217, 1283, 1815, 1935
MaeII ACGT 6 cut(s) 523, 794, 860, 1052, 1765, 2220
MaeIII GTNAC 2 cut(s) 1244, 1994
MboII GAAGA 7 cut(s) 114, 1318, 1433, 1448, 1823, 1924, 2397
MfeI CAATTG 1 cut(s) 910
MflI RGATCY 3 cut(s) 1438, 1867, 1914
MhlI GDGCHC 3 cut(s) 9, 2210, 2334
MlsI TGGCCA 1 cut(s) 891
MluNI TGGCCA 1 cut(s) 891
MlyI GAGTC 1 cut(s) 1542
MmeI TCCRAC 2 cut(s) 239, 1963
Mox20I TGGCCA 1 cut(s) 891
Mph1103I ATGCAT 2 cut(s) 2146, 2234
MscI TGGCCA 1 cut(s) 891
MseI TTAA 6 cut(s) 516, 527, 986, 2391, 2417, 2443
MslI CAYNNNNRTG 1 cut(s) 897
Msp20I TGGCCA 1 cut(s) 891
MspA1I CMGCKG 1 cut(s) 68
MspI CCGG 5 cut(s) 128, 510, 838, 971, 1059
MunI CAATTG 1 cut(s) 910
Mva1269I GAATGC 3 cut(s) 1124, 1374, 1683
MvaI CCWGG 7 cut(s) 162, 383, 480, 561, 1239, 1250, 2343
MwoI GCNNNNNNNGC 6 cut(s) 692, 1011, 1018, 1371, 2176, 2263
NciI CCSGG 3 cut(s) 129, 1059, 1060
NcoI CCATGG 1 cut(s) 647
NdeI CATATG 1 cut(s) 1990
NlaIV GGNNCC 4 cut(s) 259, 622, 1480, 2004
NmuCI GTSAC 1 cut(s) 1244
NsiI ATGCAT 2 cut(s) 2146, 2234
NspI RCATGY 1 cut(s) 1723
OliI CACNNNNGTG 1 cut(s) 897
PaeR7I CTCGAG 1 cut(s) 2432
PagI TCATGA 1 cut(s) 780
PcsI WCGNNNNNNNCGW 1 cut(s) 800
PctI GAATGC 3 cut(s) 1124, 1374, 1683
PfeI GAWTC 4 cut(s) 1044, 1108, 1841, 2128
PflMI CCANNNNNTGG 2 cut(s) 254, 653
PfoI TCCNGGA 1 cut(s) 381
PkrI GCNGC 8 cut(s) 70, 179, 269, 378, 588, 1031, 1449, 1682
PleI GAGTC 1 cut(s) 1542
PpsI GAGTC 1 cut(s) 1542
Ppu21I YACGTR 1 cut(s) 1053
Psp124BI GAGCTC 1 cut(s) 9
Psp1406I AACGTT 1 cut(s) 860
Psp6I CCWGG 7 cut(s) 160, 381, 478, 559, 1237, 1248, 2341
PspEI GGTNACC 1 cut(s) 1244
PspFI CCCAGC 1 cut(s) 145
PspGI CCWGG 7 cut(s) 160, 381, 478, 559, 1237, 1248, 2341
PspN4I GGNNCC 4 cut(s) 259, 622, 1480, 2004
PspPI GGNCC 4 cut(s) 621, 644, 825, 1529
PspXI VCTCGAGB 1 cut(s) 2432
PstI CTGCAG 1 cut(s) 378
PstNI CAGNNNCTG 3 cut(s) 33, 71, 382
PsuI RGATCY 3 cut(s) 1438, 1867, 1914
PvuII CAGCTG 1 cut(s) 68
RsaI GTAC 5 cut(s) 197, 437, 632, 765, 2223
RsaNI GTAC 5 cut(s) 196, 436, 631, 764, 2222
RseI CAYNNNNRTG 1 cut(s) 897
SacI GAGCTC 1 cut(s) 9
SalI GTCGAC 1 cut(s) 796
SaqAI TTAA 6 cut(s) 516, 527, 986, 2391, 2417, 2443
SatI GCNGC 8 cut(s) 69, 178, 268, 377, 587, 1030, 1448, 1681
Sau96I GGNCC 4 cut(s) 621, 644, 825, 1529
ScaI AGTACT 2 cut(s) 437, 632
SchI GAGTC 1 cut(s) 1542
SduI GDGCHC 3 cut(s) 9, 2210, 2334
SfaNI GCATC 5 cut(s) 409, 1334, 1341, 2219, 2241
SfcI CTRYAG 3 cut(s) 374, 2261, 2292
Sfr274I CTCGAG 1 cut(s) 2432
SgrDI CGTCGACG 1 cut(s) 796
SinI GGWCC 3 cut(s) 621, 825, 1529
SlaI CTCGAG 1 cut(s) 2432
SmaI CCCGGG 1 cut(s) 1060
SmiMI CAYNNNNRTG 1 cut(s) 897
SmlI CTYRAG 4 cut(s) 502, 634, 1112, 2432
SmoI CTYRAG 4 cut(s) 502, 634, 1112, 2432
SsiI CCGC 3 cut(s) 178, 1521, 2122
SspI AATATT 1 cut(s) 138
SspMI CTAG 4 cut(s) 1217, 1283, 1815, 1935
SstI GAGCTC 1 cut(s) 9
StyI CCWWGG 2 cut(s) 647, 681
TaaI ACNGT 4 cut(s) 220, 325, 351, 896
TaiI ACGT 6 cut(s) 526, 797, 863, 1055, 1768, 2223
TaqI TCGA 8 cut(s) 520, 744, 797, 808, 1209, 1221, 1458, 2433
TatI WGTACW 3 cut(s) 435, 630, 763
TauI GCSGC 1 cut(s) 180
TfiI GAWTC 4 cut(s) 1044, 1108, 1841, 2128
Tru1I TTAA 6 cut(s) 516, 527, 986, 2391, 2417, 2443
Tru9I TTAA 6 cut(s) 516, 527, 986, 2391, 2417, 2443
TscAI CASTG 6 cut(s) 106, 268, 673, 899, 1195, 2040
TseFI GTSAC 1 cut(s) 1244
TseI GCWGC 7 cut(s) 68, 267, 376, 586, 1029, 1447, 1680
Tsp45I GTSAC 1 cut(s) 1244
TspGWI ACGGA 1 cut(s) 2388
TspMI CCCGGG 1 cut(s) 1058
TspRI CASTG 6 cut(s) 106, 268, 673, 899, 1195, 2040
Van91I CCANNNNNTGG 2 cut(s) 254, 653
VneI GTGCAC 1 cut(s) 2206
VpaK11BI GGWCC 3 cut(s) 621, 825, 1529
XagI CCTNNNNNAGG 1 cut(s) 558
XapI RAATTY 3 cut(s) 57, 189, 337
XceI RCATGY 1 cut(s) 1723
XcmI CCANNNNNNNNNTGG 3 cut(s) 222, 1732, 1802
XhoI CTCGAG 1 cut(s) 2432
XmaI CCCGGG 1 cut(s) 1058
XmiI GTMKAC 4 cut(s) 332, 797, 1536, 1546
XspI CTAG 4 cut(s) 1217, 1283, 1815, 1935
ZrmI AGTACT 2 cut(s) 437, 632
Zsp2I ATGCAT 2 cut(s) 2146, 2234
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.