Rroxscaffold_1G00052560

divergent subfamily of APPLE domains

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000207
Physical Location & Seq
Forward (+)
73338094 .. 73341785
3692 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00052560.1

Sequence Viewer

Length: 2493 bp
ATGAAAACCATTTTTCTGTGCTTGTTCTTATTTTCCTTCCTAAGAACCTCAAATACACTAGACTCTATCACTCCAACTCAATTTATTAAAGATGGTCAAACTCTGGTTTCAGCTGGTGGAAGCTATGAACTGGGATTCTTTAGTCCCGGTGAAGTGAAGGGCAGATACTTGGGAATATGGTACACCTTTTCTACTGAGACAGTGGTATGGGTAGCCAACAGAGAAGTACCACTTGATGATTCTTCAGGAGTTTTAAAGGTCACTGATCAGGGAGTTTTAGTCCTTCTCAATAGCTCAAATGGCACTGCATGGTCCTCCAACTTATTGAGAACTGCAGGGAATCCAGTCTCGCAACTCTTGGATTCGGGAAATCTTGTTGTGAAAGATGGAAAAGAAACTAATCCTGATAACTTCTTGTGGCAGAGTTTTGATTATCCTTGTGATACACACCTGCCAGAAATGAAGCTTGGTTGGAATTTAGTTACTGGTTTAGATAGGTATGTCTCGTCTTGGAGGAGCACAGAAGATCCTGCTCAAGGAGACTTTTCACTACTGATGGATACTCATGGTTTCCCACAGATTTTTATTATGAATGGAGCTAAGATACTGACTAGAGCAGGGCCATGGAACGGCGTTGGATTAACTGGATATCAAGGAAGGCCAAATCCAGTAGCAAATTTTGAATTTGTGTTGACTAAGAATGAAGTCTATTTTGAGTACACACTCATCAACAGGTCAATGTTTGCAAGATTTGCGTTGAACCCGTACGGCCTTGCACAGTGGTTTACCTGGACAGATTACTCACATAGTTGGGAACCTTTCGTGTCATCCCAAGCAGATCAGTGTGATAATTATGCCTTTTGTGGTGCTAATGCTAGATGTAATGTCAGTAATACTCCTGTATGTGCATGCTTGAATGGGTTTGTACCAAAATCTCCGAAAGATTGGAACTCAACAAATTGGTCTGAGGGATGTGTTCGGAAGACTCCATTAGCTTGCAGCTCTGCAGATGGCTTCTCAAAGTACAGTAACTTTAAACTGCCGGACACATCTTCTTCCTGGTATGACAAGAGCATGAGCCTTAAGGAATGCCAGGGATTATGTTTGCGAAACTGTTCATGTACTGCATGTGCAAATTTAGATGTCAGGGAAGGTGGAAGTGGCTGCTTGCTTTGGTTTGGGAACCTCAATGACATCAGAGAATTCACCTCTGATTATCAAGGCCTCTATATACGGCTGGCTGCTTCAGACCTAGGCCAGCGATATAGGAGATTGTCTGCTCTAGTGCCTTATCCTATTGTGAAAAAGAGCAAGTCCAGCAAGACAAAGCTAGCAGGAATTCTGATCGGCTCACCTGTACTTCTTGTGGGAATGCTAATAGTTGGGTTTGTTTTGTATAAACGGAAGAGGAAACTCAGAAAGCAAGAAGCCAGAAGAAAGCTGGATTCCAGAAAGGGGGATTACCGTGGAGAAGACCGGGAAGACTTGGAGTTACCACTATTTGACTTGATCACTATTGCTACTGCCACTAATGACTTTTCAAGCAGCAACAAGCTGGGTGAAGGTGGTTTTGGACCTGTGTACAAGGGAACTTTGGTTGGAGGAGAGGAAATTGCAGTAAAGAGGCTCTCAAAGAACTCTGGACAGGGATTGAGAGAGTTTAAAAACGAAGTTCTACTGATAGCCAAACTTCAGCATAGGAATCTTGTAAAGCTTCTTGGTTGTTGCATTCAAGAAGATGAAGAAATTTTAGTATATGAATTCATGCCTAACAGAAGCTTGGACTTCTTTATCTTTGATCAAGAAAGACAGAAGTTTCTCGACTGGCCCACGTGCTTCCACATTATTGACGGAATTGCTCGAGGGCTTCTTTATCTCCATCAAGACTCTAGATTAAGAATTATCCATAGAGATATGAAGGCTAGCAATATCTTGCTAGACAATGATATGAACCCAAAGATTTCGGACTTCGGCCTGGCTAAAACATTTGGGGGTGATCAAAGTGAAGACAGTACCAATAGGGTAGTTGGAACACATGGTTATATGGCTCCTGAATATGCAGTAGATGGAACTTTCTCAATGAAATCTGATGTATTTAGCTTTGGAGTTATTATGCTAGAGATATTGAGCAGGAAGAAGAACAGGGGATTTTGTCATCCAGATCACCGTCTTAACCTTCTTGGACATGCATGGACACTATGGAATCAAAATAATCCTCTGGAAATGGTTGATGTGACGTTATGTGATTCTTGCAACATAACCGAAGTGTTAAGGTGTCTTCATGTGGGCCTGTTATGTGTGCAACAACTACCTGAAGATAGACCAAGCATGTCATCTGTGGTTCTAATGTTGGGCAGTGAAGTTAAGTTGCCTTCACCAAAGCAGCCTGGTTTTTACACTGGACGGACTGTGACAGTCCCCAAATCAACATCAACAACGACCGACTTGTTTTCAACAAATAATTGCAGCACAATGTTTCTAGAGGCTCGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

830

Amino Acids

92.98

Weight (kDa)

7.41

Isoelectric Point (pI)

39.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 67 - 172 1.6e-36 D-mannose binding lectin
S_locus_glycop PF00954 205 - 312 2.6e-24 S-locus glycoprotein domain
PAN_2 PF08276 333 - 399 8.9e-19 PAN-like domain
Pkinase PF00069 514 - 713 1e-43 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 516 - 781 8.2e-46 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000207)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G27300
fragaria_vesca FvH4_3g15930 FvH4_3g15980 FvH4_3g15980 FvH4_3g15980 FvH4_3g15980 FvH4_3g21350 FvH4_3g21400 FvH4_3g21400 FvH4_6g12332
malus_domestica MD02G1167100.v1.1 MD02G1167200.v1.1 MD03G1185600.v1.1 MD05G1213900.v1.1 MD05G1214100.v1.1 MD05G1214200.v1.1 MD05G1214700.v1.1 MD05G1216300.v1.1 MD05G1216800.v1.1 MD05G1217300.v1.1 MD05G1218000.v1.1 MD11G1231100.v1.1 MD11G1231200.v1.1 MD11G1231400.v1.1 MD11G1231500.v1.1 MD11G1232400.v1.1 MD11G1232500.v1.1
prunus_persica Prupe.4G142300_v2.0.a1 Prupe.4G142400_v2.0.a1 Prupe.4G142500_v2.0.a1 Prupe.4G142600_v2.0.a1 Prupe.4G142800_v2.0.a1 Prupe.4G195000_v2.0.a1 Prupe.4G195000_v2.0.a1 Prupe.4G195000_v2.0.a1 Prupe.4G195100_v2.0.a1 Prupe.4G195300_v2.0.a1 Prupe.4G195300_v2.0.a1 Prupe.4G195300_v2.0.a1 Prupe.4G195400_v2.0.a1 Prupe.4G195400_v2.0.a1 Prupe.4G195500_v2.0.a1 Prupe.4G195700_v2.0.a1 Prupe.4G195900_v2.0.a1
pyrus_communis pycom03g16540 pycom05g19920 pycom11g20460 pycom11g20480 pycom11g20490 pycom11g20500 pycom11g20530
rosa_chinensis RchiOBHm_Chr2g0139311 RchiOBHm_Chr2g0139351 RchiOBHm_Chr5g0026801 RchiOBHm_Chr5g0026851 RchiOBHm_Chr5g0026891 RchiOBHm_Chr5g0026911 RchiOBHm_Chr5g0036571 RchiOBHm_Chr5g0036591 RchiOBHm_Chr5g0036721 RchiOBHm_Chr5g0036741 RchiOBHm_Chr7g0222981
rosa_laevigata RLG00000002006 RLG00000019807 RLG00000032964 RLG00000032968 RLG00000032969 RLG00000033706 RLG00000033708 RLG00000033712 RLG00000033716
rosa_multiflora Rmu_co8256651.1_g000001 Rmu_co8279377.1_g000001 Rmu_co8429919.1_g000001 Rmu_co8490103.1_g000001 Rmu_sc0000774.1_g000002 Rmu_sc0002525.1_g000012 Rmu_sc0004223.1_g000008 Rmu_sc0004390.1_g000002 Rmu_sc0006059.1_g000014 Rmu_sc0006059.1_g000024 Rmu_sc0006514.1_g000020 Rmu_sc0014333.1_g000002 Rmu_ssc0000172.1_g000018 Rmu_ssc0000172.1_g000029
rosa_roxburghii Rroxscaffold_1G00044230 Rroxscaffold_1G00044260 Rroxscaffold_1G00044320 Rroxscaffold_1G00044340 Rroxscaffold_1G00052560 Rroxscaffold_1G00052570 Rroxscaffold_1G00052660 Rroxscaffold_1G00052720 Rroxscaffold_2G00105450 Rroxscaffold_2G00105460
rosa_rugosa Rorug02G0356900 Rorug02G0357000 Rorug02G0357100 Rorug02G0357200 Rorug02G0357300 Rorug02G0357400 Rorug02G0357500 Rorug02G0357600 Rorug05G0095400 Rorug05G0095500 Rorug05G0095500 Rorug05G0095600 Rorug05G0095700 Rorug05G0095800 Rorug05G0157700 Rorug05G0157800
rosa_samantha Rh2AG406800 Rh2AG406900 Rh2BG417300 Rh2CG392900 Rh2DG426200 Rh5AG189300 Rh5AG249400 Rh5AG250100 Rh5BG186300 Rh5BG186900 Rh5BG187000 Rh5BG249500 Rh5BG250600 Rh5BG250900 Rh5BG251500 Rh5BG251800 Rh5CG205500 Rh5CG206300 Rh5CG206700 Rh5CG283000 Rh5CG283300 Rh5CG283500 Rh5DG188500 Rh5DG188700 Rh5DG258700 Rh5DG258900 Rh5DG259600 Rh5DG259900 Rh7CG375800 Rh7DG352500
rosa_wichuraiana Rw2G032960 Rw2G032970 Rw2G033220 Rw2G033230 Rw5G017130 Rw5G017230 Rw5G022940 Rw5G022960 Rw5G022980 Rw7G030420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 459
Acc36I ACCTGC 1 cut(s) 459
AclWI GGATC 1 cut(s) 521
AcsI RAATTY 8 cut(s) 475, 676, 683, 1135, 1202, 1338, 1746, 1760
AcuI CTGAAG 4 cut(s) 228, 1230, 1676, 2334
AcvI CACGTG 1 cut(s) 1833
AfiI CCNNNNNNNGG 3 cut(s) 536, 629, 1455
AflII CTTAAG 1 cut(s) 1082
AgsI TTSAA 6 cut(s) 683, 760, 916, 1542, 1733, 2454
AjnI CCWGG 5 cut(s) 788, 1058, 1092, 1974, 2386
AjuI GAANNNNNNNTTGG 4 cut(s) 450, 482, 1554, 1586
Alw21I GWGCWC 1 cut(s) 521
Alw26I GTCTC 4 cut(s) 191, 352, 508, 534
AlwI GGATC 1 cut(s) 521
Ama87I CYCGRG 1 cut(s) 1860
AoxI GGCC 8 cut(s) 620, 659, 769, 1222, 1255, 1826, 1972, 2287
ApeKI GCWGC 6 cut(s) 999, 1164, 1241, 1545, 2383, 2466
ApoI RAATTY 8 cut(s) 475, 676, 683, 1135, 1202, 1338, 1746, 1760
Asp700I GAANNNNTTC 1 cut(s) 1114
AspA2I CCTAGG 1 cut(s) 1252
AspS9I GGNCC 5 cut(s) 312, 620, 1574, 1827, 2287
AsuC2I CCSGG 2 cut(s) 147, 1478
AsuHPI GGTGA 7 cut(s) 161, 1198, 1344, 1571, 2006, 2156, 2367
AsuNHI GCTAGC 2 cut(s) 1330, 1922
AvaI CYCGRG 1 cut(s) 1860
AvaII GGWCC 2 cut(s) 312, 1574
AvrII CCTAGG 1 cut(s) 1252
BarI GAAGNNNNNNTAC 2 cut(s) 149, 181
BbrPI CACGTG 1 cut(s) 1833
BbsI GAAGAC 5 cut(s) 989, 1479, 1488, 2013, 2270
Bbv12I GWGCWC 1 cut(s) 521
BbvI GCAGC 6 cut(s) 1011, 1151, 1228, 1557, 2395, 2478
BccI CCATC 6 cut(s) 86, 380, 550, 1004, 1887, 2060
BceAI ACGGC 3 cut(s) 646, 784, 1250
BciT130I CCWGG 5 cut(s) 790, 1060, 1094, 1976, 2388
BciVI GTATCC 1 cut(s) 553
BclI TGATCA 4 cut(s) 265, 1509, 1798, 1996
BcnI CCSGG 2 cut(s) 147, 1478
BcoDI GTCTC 4 cut(s) 191, 352, 508, 534
BfmI CTRYAG 2 cut(s) 333, 1005
BfrI CTTAAG 1 cut(s) 1082
BfuAI ACCTGC 1 cut(s) 459
BfuI GTATCC 1 cut(s) 553
BisI GCNGC 6 cut(s) 1000, 1165, 1242, 1546, 2384, 2467
BlnI CCTAGG 1 cut(s) 1252
BlsI GCNGC 6 cut(s) 1001, 1166, 1243, 1547, 2385, 2468
Bme1390I CCNGG 7 cut(s) 147, 790, 1060, 1094, 1478, 1976, 2388
Bme18I GGWCC 2 cut(s) 312, 1574
BmeT110I CYCGRG 1 cut(s) 1860
BmgT120I GGNCC 5 cut(s) 312, 620, 1574, 1827, 2287
BmiI GGNNCC 3 cut(s) 816, 1184, 2049
BmrFI CCNGG 7 cut(s) 147, 790, 1060, 1094, 1478, 1976, 2388
BmrI ACTGGG 1 cut(s) 140
BmtI GCTAGC 2 cut(s) 1334, 1926
BmuI ACTGGG 1 cut(s) 140
BpiI GAAGAC 5 cut(s) 989, 1479, 1488, 2013, 2270
BpuEI CTTGAG 1 cut(s) 519
BpuMI CCSGG 2 cut(s) 147, 1478
BsaAI YACGTR 1 cut(s) 1833
BsaJI CCNNGG 4 cut(s) 623, 1093, 1252, 1465
BsaXI ACNNNNNCTCC 2 cut(s) 264, 294
Bsc4I CCNNNNNNNGG 3 cut(s) 536, 629, 1455
Bse1I ACTGG 7 cut(s) 135, 344, 490, 649, 668, 1829, 2404
BseBI CCWGG 5 cut(s) 790, 1060, 1094, 1976, 2388
BseDI CCNNGG 4 cut(s) 623, 1093, 1252, 1465
BseGI GGATG 3 cut(s) 827, 977, 2155
BseLI CCNNNNNNNGG 3 cut(s) 536, 629, 1455
BseMII CTCAG 3 cut(s) 186, 957, 1429
BseNI ACTGG 7 cut(s) 135, 344, 490, 649, 668, 1829, 2404
BseRI GAGGAG 2 cut(s) 529, 1617
BseXI GCAGC 6 cut(s) 1011, 1151, 1228, 1557, 2395, 2478
BseYI CCCAGC 1 cut(s) 1555
Bsh1285I CGRYCG 1 cut(s) 2442
BshFI GGCC 8 cut(s) 622, 661, 771, 1224, 1257, 1828, 1974, 2289
BsiEI CGRYCG 1 cut(s) 2442
BsiHKAI GWGCWC 1 cut(s) 521
BsiHKCI CYCGRG 1 cut(s) 1860
BsiSI CCGG 3 cut(s) 147, 1043, 1477
BsiWI CGTACG 1 cut(s) 765
BslFI GGGAC 2 cut(s) 129, 2402
BslI CCNNNNNNNGG 3 cut(s) 536, 629, 1455
BsmAI GTCTC 4 cut(s) 191, 352, 508, 534
BsmFI GGGAC 2 cut(s) 129, 2402
BsmI GAATGC 3 cut(s) 1094, 1377, 1728
BsnI GGCC 8 cut(s) 622, 661, 771, 1224, 1257, 1828, 1974, 2289
BsoBI CYCGRG 1 cut(s) 1860
Bsp1286I GDGCHC 1 cut(s) 521
Bsp1407I TGTACA 1 cut(s) 1581
Bsp143I GATC 8 cut(s) 265, 526, 838, 1344, 1509, 1798, 1996, 2161
Bsp19I CCATGG 1 cut(s) 623
BspANI GGCC 8 cut(s) 622, 661, 771, 1224, 1257, 1828, 1974, 2289
BspCNI CTCAG 3 cut(s) 187, 958, 1428
BspLI GGNNCC 3 cut(s) 816, 1184, 2049
BspMAI CTGCAG 2 cut(s) 337, 1009
BspMI ACCTGC 1 cut(s) 459
BspOI GCTAGC 2 cut(s) 1334, 1926
BspPI GGATC 1 cut(s) 521
BspTI CTTAAG 1 cut(s) 1082
BsrGI TGTACA 1 cut(s) 1581
BsrI ACTGG 7 cut(s) 135, 344, 490, 649, 668, 1829, 2404
BssECI CCNNGG 4 cut(s) 623, 1093, 1252, 1465
BssMI GATC 8 cut(s) 265, 526, 838, 1344, 1509, 1798, 1996, 2161
BssT1I CCWWGG 2 cut(s) 623, 1252
Bst2UI CCWGG 5 cut(s) 790, 1060, 1094, 1976, 2388
Bst4CI ACNGT 9 cut(s) 202, 780, 1028, 1115, 1466, 2012, 2168, 2410, 2416
Bst6I CTCTTC 1 cut(s) 1400
BstAFI CTTAAG 1 cut(s) 1082
BstAPI GCANNNNNTGC 1 cut(s) 752
BstAUI TGTACA 1 cut(s) 1581
BstBAI YACGTR 1 cut(s) 1833
BstC8I GCNNGC 8 cut(s) 910, 997, 1169, 1239, 1259, 1332, 1924, 2488
BstDEI CTNAG 6 cut(s) 41, 195, 600, 696, 966, 1415
BstDSI CCRYGG 2 cut(s) 623, 1465
BstENI CCTNNNNNAGG 1 cut(s) 534
BstF5I GGATG 3 cut(s) 827, 977, 2155
BstKTI GATC 8 cut(s) 268, 529, 841, 1347, 1512, 1801, 1999, 2164
BstMAI GTCTC 4 cut(s) 191, 352, 508, 534
BstMBI GATC 8 cut(s) 265, 526, 838, 1344, 1509, 1798, 1996, 2161
BstMCI CGRYCG 1 cut(s) 2442
BstMWI GCNNNNNNNGC 3 cut(s) 300, 752, 1317
BstNI CCWGG 5 cut(s) 790, 1060, 1094, 1976, 2388
BstNSI RCATGY 4 cut(s) 912, 1131, 2189, 2332
BstSCI CCNGG 7 cut(s) 145, 788, 1058, 1092, 1476, 1974, 2386
BstSFI CTRYAG 2 cut(s) 333, 1005
BstV1I GCAGC 6 cut(s) 1011, 1151, 1228, 1557, 2395, 2478
BstV2I GAAGAC 5 cut(s) 989, 1479, 1488, 2013, 2270
BstX2I RGATCY 1 cut(s) 526
BstYI RGATCY 1 cut(s) 526
BsuI GTATCC 1 cut(s) 553
BsuRI GGCC 8 cut(s) 622, 661, 771, 1224, 1257, 1828, 1974, 2289
BtgI CCRYGG 2 cut(s) 623, 1465
BtsCI GGATG 3 cut(s) 827, 977, 2155
BtsI GCAGTG 2 cut(s) 303, 2362
BtsIMutI CAGTG 7 cut(s) 207, 261, 303, 785, 848, 2362, 2397
BveI ACCTGC 1 cut(s) 459
Cac8I GCNNGC 8 cut(s) 910, 997, 1169, 1239, 1259, 1332, 1924, 2488
Cfr13I GGNCC 5 cut(s) 312, 620, 1574, 1827, 2287
DdeI CTNAG 6 cut(s) 41, 195, 600, 696, 966, 1415
DpnI GATC 8 cut(s) 267, 528, 840, 1346, 1511, 1800, 1998, 2163
DpnII GATC 8 cut(s) 265, 526, 838, 1344, 1509, 1798, 1996, 2161
DraI TTTAAA 3 cut(s) 255, 1036, 1663
Eam1104I CTCTTC 1 cut(s) 1400
EarI CTCTTC 1 cut(s) 1400
Eco130I CCWWGG 2 cut(s) 623, 1252
Eco147I AGGCCT 1 cut(s) 1224
Eco32I GATATC 1 cut(s) 650
Eco47I GGWCC 2 cut(s) 312, 1574
Eco57I CTGAAG 4 cut(s) 228, 1230, 1676, 2334
Eco72I CACGTG 1 cut(s) 1833
Eco88I CYCGRG 1 cut(s) 1860
EcoNI CCTNNNNNAGG 1 cut(s) 534
EcoRI GAATTC 3 cut(s) 1202, 1338, 1760
EcoRII CCWGG 5 cut(s) 788, 1058, 1092, 1974, 2386
EcoRV GATATC 1 cut(s) 650
EcoT14I CCWWGG 2 cut(s) 623, 1252
EcoT22I ATGCAT 1 cut(s) 2191
ErhI CCWWGG 2 cut(s) 623, 1252
FalI AAGNNNNNCTT 2 cut(s) 216, 248
FaqI GGGAC 2 cut(s) 129, 2402
FbaI TGATCA 4 cut(s) 265, 1509, 1798, 1996
Fnu4HI GCNGC 6 cut(s) 1000, 1165, 1242, 1546, 2384, 2467
FokI GGATG 3 cut(s) 814, 984, 2142
Fsp4HI GCNGC 6 cut(s) 1000, 1165, 1242, 1546, 2384, 2467
GluI GCNGC 6 cut(s) 1000, 1165, 1242, 1546, 2384, 2467
GsaI CCCAGC 1 cut(s) 1559
HaeIII GGCC 8 cut(s) 622, 661, 771, 1224, 1257, 1828, 1974, 2289
HapII CCGG 3 cut(s) 147, 1043, 1477
HincII GTYRAC 1 cut(s) 693
HindII GTYRAC 1 cut(s) 693
HindIII AAGCTT 3 cut(s) 464, 1712, 1777
HpaII CCGG 3 cut(s) 147, 1043, 1477
HphI GGTGA 7 cut(s) 161, 1198, 1344, 1571, 2006, 2156, 2367
Hpy166II GTNNAC 5 cut(s) 183, 693, 720, 786, 1582
Hpy8I GTNNAC 5 cut(s) 183, 693, 720, 786, 1582
HpyAV CCTTC 9 cut(s) 46, 151, 293, 651, 1145, 1556, 1912, 2186, 2382
HpyCH4III ACNGT 9 cut(s) 202, 780, 1028, 1115, 1466, 2012, 2168, 2410, 2416
HpyCH4IV ACGT 2 cut(s) 1832, 2237
HpyF10VI GCNNNNNNNGC 3 cut(s) 300, 752, 1317
HpyF3I CTNAG 6 cut(s) 41, 195, 600, 696, 966, 1415
HpySE526I ACGT 2 cut(s) 1832, 2237
Ksp22I TGATCA 4 cut(s) 265, 1509, 1798, 1996
Kzo9I GATC 8 cut(s) 265, 526, 838, 1344, 1509, 1798, 1996, 2161
LmnI GCTCC 3 cut(s) 516, 596, 2053
Lsp1109I GCAGC 6 cut(s) 1011, 1151, 1228, 1557, 2395, 2478
MaeII ACGT 2 cut(s) 1832, 2237
MaeIII GTNAC 6 cut(s) 259, 481, 1028, 1491, 2233, 2410
MalI GATC 8 cut(s) 267, 528, 840, 1346, 1511, 1800, 1998, 2163
MboI GATC 8 cut(s) 265, 526, 838, 1344, 1509, 1798, 1996, 2161
MflI RGATCY 1 cut(s) 526
MhlI GDGCHC 1 cut(s) 521
MlyI GAGTC 3 cut(s) 56, 979, 1880
MmeI TCCRAC 6 cut(s) 98, 342, 452, 616, 1579, 2008
Mph1103I ATGCAT 1 cut(s) 2191
MroXI GAANNNNTTC 1 cut(s) 1114
MspA1I CMGCKG 1 cut(s) 113
MspCI CTTAAG 1 cut(s) 1082
MspI CCGG 3 cut(s) 147, 1043, 1477
MspR9I CCNGG 7 cut(s) 147, 790, 1060, 1094, 1478, 1976, 2388
Mva1269I GAATGC 3 cut(s) 1094, 1377, 1728
MvaI CCWGG 5 cut(s) 790, 1060, 1094, 1976, 2388
MwoI GCNNNNNNNGC 3 cut(s) 300, 752, 1317
NciI CCSGG 2 cut(s) 147, 1478
NcoI CCATGG 1 cut(s) 623
NdeII GATC 8 cut(s) 265, 526, 838, 1344, 1509, 1798, 1996, 2161
NheI GCTAGC 2 cut(s) 1330, 1922
NlaIV GGNNCC 3 cut(s) 816, 1184, 2049
NmuCI GTSAC 3 cut(s) 259, 2233, 2410
NsiI ATGCAT 1 cut(s) 2191
NspI RCATGY 4 cut(s) 912, 1131, 2189, 2332
PaeI GCATGC 1 cut(s) 912
PaeR7I CTCGAG 1 cut(s) 1860
PaqCI CACCTGC 1 cut(s) 459
PceI AGGCCT 1 cut(s) 1224
PctI GAATGC 3 cut(s) 1094, 1377, 1728
PdmI GAANNNNTTC 1 cut(s) 1114
PfeI GAWTC 8 cut(s) 135, 239, 340, 362, 1445, 1702, 2203, 2246
Pfl23II CGTACG 1 cut(s) 765
PkrI GCNGC 6 cut(s) 1001, 1166, 1243, 1547, 2385, 2468
PleI GAGTC 3 cut(s) 56, 979, 1880
PmaCI CACGTG 1 cut(s) 1833
PmlI CACGTG 1 cut(s) 1833
PpsI GAGTC 3 cut(s) 56, 979, 1880
Ppu21I YACGTR 1 cut(s) 1833
Psp6I CCWGG 5 cut(s) 788, 1058, 1092, 1974, 2386
PspCI CACGTG 1 cut(s) 1833
PspFI CCCAGC 1 cut(s) 1555
PspGI CCWGG 5 cut(s) 788, 1058, 1092, 1974, 2386
PspLI CGTACG 1 cut(s) 765
PspN4I GGNNCC 3 cut(s) 816, 1184, 2049
PspPI GGNCC 5 cut(s) 312, 620, 1574, 1827, 2287
PspXI VCTCGAGB 1 cut(s) 1860
PsrI GAACNNNNNNTAC 2 cut(s) 37, 69
PstI CTGCAG 2 cut(s) 337, 1009
PsuI RGATCY 1 cut(s) 526
PvuII CAGCTG 1 cut(s) 113
SatI GCNGC 6 cut(s) 1000, 1165, 1242, 1546, 2384, 2467
Sau3AI GATC 8 cut(s) 265, 526, 838, 1344, 1509, 1798, 1996, 2161
Sau96I GGNCC 5 cut(s) 312, 620, 1574, 1827, 2287
SchI GAGTC 3 cut(s) 56, 979, 1880
ScrFI CCNGG 7 cut(s) 147, 790, 1060, 1094, 1478, 1976, 2388
SduI GDGCHC 1 cut(s) 521
SfcI CTRYAG 2 cut(s) 333, 1005
Sfr274I CTCGAG 1 cut(s) 1860
SinI GGWCC 2 cut(s) 312, 1574
SlaI CTCGAG 1 cut(s) 1860
SmlI CTYRAG 3 cut(s) 534, 1082, 1860
SmoI CTYRAG 3 cut(s) 534, 1082, 1860
SphI GCATGC 1 cut(s) 912
SseBI AGGCCT 1 cut(s) 1224
StuI AGGCCT 1 cut(s) 1224
StyD4I CCNGG 7 cut(s) 145, 788, 1058, 1092, 1476, 1974, 2386
StyI CCWWGG 2 cut(s) 623, 1252
TaaI ACNGT 9 cut(s) 202, 780, 1028, 1115, 1466, 2012, 2168, 2410, 2416
TaiI ACGT 2 cut(s) 1835, 2240
TaqI TCGA 2 cut(s) 1821, 1861
TaqII GACCGA 1 cut(s) 2456
TatI WGTACW 5 cut(s) 717, 1023, 1121, 1357, 1581
TfiI GAWTC 8 cut(s) 135, 239, 340, 362, 1445, 1702, 2203, 2246
TscAI CASTG 7 cut(s) 207, 268, 310, 785, 848, 2362, 2404
TseFI GTSAC 3 cut(s) 259, 2233, 2410
TseI GCWGC 6 cut(s) 999, 1164, 1241, 1545, 2383, 2466
Tsp45I GTSAC 3 cut(s) 259, 2233, 2410
TspGWI ACGGA 3 cut(s) 1417, 1866, 2419
TspRI CASTG 7 cut(s) 207, 268, 310, 785, 848, 2362, 2404
Vha464I CTTAAG 1 cut(s) 1082
VpaK11BI GGWCC 2 cut(s) 312, 1574
XagI CCTNNNNNAGG 1 cut(s) 534
XapI RAATTY 8 cut(s) 475, 676, 683, 1135, 1202, 1338, 1746, 1760
XbaI TCTAGA 2 cut(s) 1889, 2479
XceI RCATGY 4 cut(s) 912, 1131, 2189, 2332
XcmI CCANNNNNNNNNTGG 1 cut(s) 1438
XhoI CTCGAG 1 cut(s) 1860
XmaJI CCTAGG 1 cut(s) 1252
XmnI GAANNNNTTC 1 cut(s) 1114
Zsp2I ATGCAT 1 cut(s) 2191
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.