Rroxscaffold_1G00052570

divergent subfamily of APPLE domains

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000207
Physical Location & Seq
Forward (+)
73370713 .. 73373594
2882 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00052570.1

Sequence Viewer

Length: 2010 bp
ATGCAAACCATGAAAACCCTTTTTGTGTGCTTGTTCTTATTTTCCTTCCTAAGAACCTCAAATACACTGGACTCTATCACTCCAACTCAATTTATTAAAGATGGTCAAACTCTGGTTTCAGCAGGTGGAAGCTATGAACTGGGATTCTTTAGTCCTGGGAAAGTGAAAGGCAGGTACTTGGGAATATGGTACACCTTTTCTACTGAGACAGTTGTATGGGTAGCGAACAGAGAAATACCACTTGATGATTCTTTAGGAGTTTTAAAGGTCACTGATCAAGGAGTTTTAGTCCTTCTCAATAGCTCAAATGGCACTGTATGGTCATCCAATTCATCAAGAATCGCAGGGAATCCAGTCTCGCAACTCTTGGATTCGGGGAATCTTGTTGTGAAAGATGGAAATGAAACTAATCCTGATAACTTCTTGTGGCAGAGTTTTGATTATCCTTGTGATACACGCCTGCCAGAAATGAAGCTTGGTTGGAATTTAGTTACTGGTTTAGATAGGTATGTCTCGTCTTGGAGGAGCACAGAAGATCCTTCTCAAGGAGAGTTTTCACTACGGATGGATACTCGTGGTTTCCCACAGATTTTTATTATGAAGGGAGCTAAGATACTGACTAGAGCAGGGACATGGAACGGCGTTGGATTAACTGGATATCAAGGAAAGCCAAATCCAGTAGCGGTTTTTGAGTTTGTGTTGACTAAGGATGAAGTCTATTATGAGTACACACTCCTCAACCGGTCAATGTTTGCAAGATATGTATTAAACCCATATGGCATTGCACAGTGGCTTACTTGGACAGACCACTCACGTAGTTGGGAACCTTTCATCTCAACTCAGGCAGATCAGTGTGAAAACTATGCCTATTGTGGTGCTAATGCTAGATGTAATGTCAGTAACACTCCTGTATGTACATGCTTGGAAGGGTTTGTACCAAAATCTCCGAAAGATTGGAACTCAACAAATTGGTCTGGGGGATGTGTTCGGGAGACTCCATTAGCTTGCAGCTTTACAGATGGCTTCTCAAAGTACAGCAACTTTAAATTGCCAGACACAACTTCTTCCTGGTATGACAAGAGCAAGACCCTCAAGGAGTGCCAGGGATTATGTTTGGGAAACTGTTCATGTTCTGCATATGCAAATTTAGATGTCAGGGAAGGTGGAAGTGGCTGCTTGCTTTGGTTTGGGAACCTCAATGACATTAGAGAATTCACCTCTGATTCTCAAGACCTCTTTATACGGCTGGCTGCATCAGACCTAGATCCTATTGTGAGAAAGAGCAAGTCCAGCAACAAAAAGCTAGCAGGAATTCTGATCGGCTCATCTGTATTTCTTGTGGGAATGCTAATAGTTGCGTTCATTCTGTATAAACGGAAGAGGAAACTCAGAAAGCAAGGAGCCAGAAGAAAGCTGGATTGCAGAAGGGAGGATTACCGTGGAGAAGACAGGGAAGACTTGGAGTTACCACTATTTGACCTGACCACTATTGCTAATGCCACTAATGACTTTTCAAGCAGCAACAAGCTGGGTGAAGGTGGTTTTGGACCTGTGTACAAGGGAACATTGATTGGAGGAGAGGAAATTGCAGTAAAGAGGCTCTCAAAAAATTCTGGACAGGGATTGAGAGAGTTCAAAAACGAAGTTCTACTGATAGCCAAACTTCAGCATCGGAATCTTGTAAAGCTTCTTGGTTGTTGCATTCAAGAAGATGAAGAAATTCTAGTATATGAATACATGCCTAACAGAAGCTTGGACTTCTTTATATTTGATCAAGAAAGACAGAAGTTGCTTGACTGGCCCGCATGCTTCCATATTATTGAGGGAATTGCTCGAGGGCTTCTTTATCTCCATCAAGACTCTAGATTAAGAATTATCCATAGAGATATGAAGGCTAGCAATATCTTGCTGGACAATGATATGAACCCAAAGATTTCAGACTTCGGTCTGGCTAAAACATTTGGGGGTGATCAAAGTGAAGACAGTACAAATAGAGTGGTGGGAACATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

669

Amino Acids

75.2

Weight (kDa)

6.93

Isoelectric Point (pI)

37.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 70 - 175 6.8e-37 D-mannose binding lectin
S_locus_glycop PF00954 208 - 315 1.9e-23 S-locus glycoprotein domain
PAN_2 PF08276 336 - 402 1.5e-20 PAN-like domain
Pkinase PF00069 505 - 669 5.4e-32 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 507 - 660 1.4e-32 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000207)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G27300
fragaria_vesca FvH4_3g15930 FvH4_3g15980 FvH4_3g15980 FvH4_3g15980 FvH4_3g15980 FvH4_3g21350 FvH4_3g21400 FvH4_3g21400 FvH4_6g12332
malus_domestica MD02G1167100.v1.1 MD02G1167200.v1.1 MD03G1185600.v1.1 MD05G1213900.v1.1 MD05G1214100.v1.1 MD05G1214200.v1.1 MD05G1214700.v1.1 MD05G1216300.v1.1 MD05G1216800.v1.1 MD05G1217300.v1.1 MD05G1218000.v1.1 MD11G1231100.v1.1 MD11G1231200.v1.1 MD11G1231400.v1.1 MD11G1231500.v1.1 MD11G1232400.v1.1 MD11G1232500.v1.1
prunus_persica Prupe.4G142300_v2.0.a1 Prupe.4G142400_v2.0.a1 Prupe.4G142500_v2.0.a1 Prupe.4G142600_v2.0.a1 Prupe.4G142800_v2.0.a1 Prupe.4G195000_v2.0.a1 Prupe.4G195000_v2.0.a1 Prupe.4G195000_v2.0.a1 Prupe.4G195100_v2.0.a1 Prupe.4G195300_v2.0.a1 Prupe.4G195300_v2.0.a1 Prupe.4G195300_v2.0.a1 Prupe.4G195400_v2.0.a1 Prupe.4G195400_v2.0.a1 Prupe.4G195500_v2.0.a1 Prupe.4G195700_v2.0.a1 Prupe.4G195900_v2.0.a1
pyrus_communis pycom03g16540 pycom05g19920 pycom11g20460 pycom11g20480 pycom11g20490 pycom11g20500 pycom11g20530
rosa_chinensis RchiOBHm_Chr2g0139311 RchiOBHm_Chr2g0139351 RchiOBHm_Chr5g0026801 RchiOBHm_Chr5g0026851 RchiOBHm_Chr5g0026891 RchiOBHm_Chr5g0026911 RchiOBHm_Chr5g0036571 RchiOBHm_Chr5g0036591 RchiOBHm_Chr5g0036721 RchiOBHm_Chr5g0036741 RchiOBHm_Chr7g0222981
rosa_laevigata RLG00000002006 RLG00000019807 RLG00000032964 RLG00000032968 RLG00000032969 RLG00000033706 RLG00000033708 RLG00000033712 RLG00000033716
rosa_multiflora Rmu_co8256651.1_g000001 Rmu_co8279377.1_g000001 Rmu_co8429919.1_g000001 Rmu_co8490103.1_g000001 Rmu_sc0000774.1_g000002 Rmu_sc0002525.1_g000012 Rmu_sc0004223.1_g000008 Rmu_sc0004390.1_g000002 Rmu_sc0006059.1_g000014 Rmu_sc0006059.1_g000024 Rmu_sc0006514.1_g000020 Rmu_sc0014333.1_g000002 Rmu_ssc0000172.1_g000018 Rmu_ssc0000172.1_g000029
rosa_roxburghii Rroxscaffold_1G00044230 Rroxscaffold_1G00044260 Rroxscaffold_1G00044320 Rroxscaffold_1G00044340 Rroxscaffold_1G00052560 Rroxscaffold_1G00052570 Rroxscaffold_1G00052660 Rroxscaffold_1G00052720 Rroxscaffold_2G00105450 Rroxscaffold_2G00105460
rosa_rugosa Rorug02G0356900 Rorug02G0357000 Rorug02G0357100 Rorug02G0357200 Rorug02G0357300 Rorug02G0357400 Rorug02G0357500 Rorug02G0357600 Rorug05G0095400 Rorug05G0095500 Rorug05G0095500 Rorug05G0095600 Rorug05G0095700 Rorug05G0095800 Rorug05G0157700 Rorug05G0157800
rosa_samantha Rh2AG406800 Rh2AG406900 Rh2BG417300 Rh2CG392900 Rh2DG426200 Rh5AG189300 Rh5AG249400 Rh5AG250100 Rh5BG186300 Rh5BG186900 Rh5BG187000 Rh5BG249500 Rh5BG250600 Rh5BG250900 Rh5BG251500 Rh5BG251800 Rh5CG205500 Rh5CG206300 Rh5CG206700 Rh5CG283000 Rh5CG283300 Rh5CG283500 Rh5DG188500 Rh5DG188700 Rh5DG258700 Rh5DG258900 Rh5DG259600 Rh5DG259900 Rh7CG375800 Rh7DG352500
rosa_wichuraiana Rw2G032960 Rw2G032970 Rw2G033220 Rw2G033230 Rw5G017130 Rw5G017230 Rw5G022940 Rw5G022960 Rw5G022980 Rw7G030420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 113
Acc36I ACCTGC 2 cut(s) 113, 162
AciI CCGC 2 cut(s) 683, 1803
AclWI GGATC 2 cut(s) 530, 1259
AcsI RAATTY 6 cut(s) 484, 1144, 1211, 1311, 1609, 1719
AcuI CTGAAG 1 cut(s) 1649
AfaI GTAC 8 cut(s) 176, 191, 728, 916, 936, 1034, 1556, 1987
AfiI CCNNNNNNNGG 1 cut(s) 545
AgeI ACCGGT 1 cut(s) 741
AgsI TTSAA 3 cut(s) 1515, 1636, 1706
AjnI CCWGG 3 cut(s) 154, 1067, 1101
AjuI GAANNNNNNNTTGG 4 cut(s) 459, 491, 1527, 1559
Alw21I GWGCWC 1 cut(s) 530
Alw26I GTCTC 4 cut(s) 200, 361, 517, 986
AlwI GGATC 2 cut(s) 530, 1259
Ama87I CYCGRG 1 cut(s) 1833
AoxI GGCC 1 cut(s) 1799
ApeKI GCWGC 4 cut(s) 1008, 1173, 1250, 1518
ApoI RAATTY 6 cut(s) 484, 1144, 1211, 1311, 1609, 1719
AsiGI ACCGGT 1 cut(s) 741
Asp700I GAANNNNTTC 2 cut(s) 1123, 1719
AspS9I GGNCC 2 cut(s) 1547, 1800
AsuHPI GGTGA 3 cut(s) 1207, 1544, 1979
AsuNHI GCTAGC 2 cut(s) 1303, 1895
AvaI CYCGRG 1 cut(s) 1833
AvaII GGWCC 1 cut(s) 1547
BarI GAAGNNNNNNTAC 2 cut(s) 918, 950
BauI CACGAG 1 cut(s) 573
BbsI GAAGAC 3 cut(s) 1452, 1461, 1986
Bbv12I GWGCWC 1 cut(s) 530
BbvI GCAGC 4 cut(s) 1020, 1160, 1237, 1530
BccI CCATC 5 cut(s) 95, 389, 559, 1013, 1860
BceAI ACGGC 2 cut(s) 655, 1259
BciT130I CCWGG 3 cut(s) 156, 1069, 1103
BciVI GTATCC 1 cut(s) 562
BclI TGATCA 3 cut(s) 274, 1771, 1969
BcoDI GTCTC 4 cut(s) 200, 361, 517, 986
BfaI CTAG 7 cut(s) 621, 885, 1262, 1304, 1724, 1863, 1896
BfuAI ACCTGC 2 cut(s) 113, 162
BfuI GTATCC 1 cut(s) 562
BisI GCNGC 4 cut(s) 1009, 1174, 1251, 1519
BlsI GCNGC 4 cut(s) 1010, 1175, 1252, 1520
Bme1390I CCNGG 3 cut(s) 156, 1069, 1103
Bme18I GGWCC 1 cut(s) 1547
BmeT110I CYCGRG 1 cut(s) 1833
BmgT120I GGNCC 2 cut(s) 1547, 1800
BmiI GGNNCC 3 cut(s) 825, 1193, 1402
BmrFI CCNGG 3 cut(s) 156, 1069, 1103
BmrI ACTGGG 1 cut(s) 149
BmsI GCATC 2 cut(s) 1262, 1678
BmtI GCTAGC 2 cut(s) 1307, 1899
BmuI ACTGGG 1 cut(s) 149
BoxI GACNNNNGTC 1 cut(s) 1944
BpiI GAAGAC 3 cut(s) 1452, 1461, 1986
BpuEI CTTGAG 3 cut(s) 528, 1076, 1212
BsaAI YACGTR 1 cut(s) 815
BsaJI CCNNGG 3 cut(s) 155, 1102, 1438
BsaWI WCCGGW 1 cut(s) 741
BsaXI ACNNNNNCTCC 2 cut(s) 273, 303
Bsc4I CCNNNNNNNGG 1 cut(s) 545
Bse118I RCCGGY 1 cut(s) 741
Bse1I ACTGG 7 cut(s) 72, 144, 353, 499, 658, 677, 1802
Bse3DI GCAATG 1 cut(s) 780
BseBI CCWGG 3 cut(s) 156, 1069, 1103
BseDI CCNNGG 3 cut(s) 155, 1102, 1438
BseGI GGATG 4 cut(s) 323, 570, 715, 986
BseLI CCNNNNNNNGG 1 cut(s) 545
BseMI GCAATG 1 cut(s) 780
BseMII CTCAG 3 cut(s) 195, 854, 1402
BseNI ACTGG 7 cut(s) 72, 144, 353, 499, 658, 677, 1802
BseRI GAGGAG 3 cut(s) 538, 725, 1590
BseXI GCAGC 4 cut(s) 1020, 1160, 1237, 1530
BseYI CCCAGC 1 cut(s) 1528
BshFI GGCC 1 cut(s) 1801
BshTI ACCGGT 1 cut(s) 741
BsiHKAI GWGCWC 1 cut(s) 530
BsiHKCI CYCGRG 1 cut(s) 1833
BsiSI CCGG 1 cut(s) 742
BslFI GGGAC 1 cut(s) 643
BslI CCNNNNNNNGG 1 cut(s) 545
BsmAI GTCTC 4 cut(s) 200, 361, 517, 986
BsmFI GGGAC 1 cut(s) 643
BsmI GAATGC 2 cut(s) 1350, 1701
BsnI GGCC 1 cut(s) 1801
BsoBI CYCGRG 1 cut(s) 1833
Bsp1286I GDGCHC 1 cut(s) 530
Bsp1407I TGTACA 2 cut(s) 914, 1554
Bsp143I GATC 7 cut(s) 274, 535, 847, 1264, 1317, 1771, 1969
BspACI CCGC 2 cut(s) 683, 1803
BspANI GGCC 1 cut(s) 1801
BspCNI CTCAG 3 cut(s) 196, 853, 1401
BspLI GGNNCC 3 cut(s) 825, 1193, 1402
BspMI ACCTGC 2 cut(s) 113, 162
BspOI GCTAGC 2 cut(s) 1307, 1899
BspPI GGATC 2 cut(s) 530, 1259
BsrDI GCAATG 1 cut(s) 780
BsrFI RCCGGY 1 cut(s) 741
BsrGI TGTACA 2 cut(s) 914, 1554
BsrI ACTGG 7 cut(s) 72, 144, 353, 499, 658, 677, 1802
BssAI RCCGGY 1 cut(s) 741
BssECI CCNNGG 3 cut(s) 155, 1102, 1438
BssMI GATC 7 cut(s) 274, 535, 847, 1264, 1317, 1771, 1969
BssSI CACGAG 1 cut(s) 573
Bst2BI CACGAG 1 cut(s) 573
Bst2UI CCWGG 3 cut(s) 156, 1069, 1103
Bst4CI ACNGT 6 cut(s) 211, 316, 789, 1124, 1439, 1985
Bst6I CTCTTC 1 cut(s) 1373
BstAUI TGTACA 2 cut(s) 914, 1554
BstBAI YACGTR 1 cut(s) 815
BstC8I GCNNGC 8 cut(s) 461, 1006, 1178, 1248, 1305, 1803, 1807, 1897
BstDEI CTNAG 6 cut(s) 50, 204, 609, 705, 840, 1388
BstDSI CCRYGG 1 cut(s) 1438
BstENI CCTNNNNNAGG 1 cut(s) 543
BstF5I GGATG 4 cut(s) 323, 570, 715, 986
BstKTI GATC 7 cut(s) 277, 538, 850, 1267, 1320, 1774, 1972
BstMAI GTCTC 4 cut(s) 200, 361, 517, 986
BstMBI GATC 7 cut(s) 274, 535, 847, 1264, 1317, 1771, 1969
BstMWI GCNNNNNNNGC 3 cut(s) 309, 1290, 1798
BstNI CCWGG 3 cut(s) 156, 1069, 1103
BstNSI RCATGY 3 cut(s) 921, 1741, 1809
BstPAI GACNNNNGTC 1 cut(s) 1944
BstSCI CCNGG 3 cut(s) 154, 1067, 1101
BstV1I GCAGC 4 cut(s) 1020, 1160, 1237, 1530
BstV2I GAAGAC 3 cut(s) 1452, 1461, 1986
BstX2I RGATCY 2 cut(s) 535, 1264
BstYI RGATCY 2 cut(s) 535, 1264
BsuI GTATCC 1 cut(s) 562
BsuRI GGCC 1 cut(s) 1801
BtgI CCRYGG 1 cut(s) 1438
BtsCI GGATG 4 cut(s) 323, 570, 715, 986
BtsIMutI CAGTG 5 cut(s) 65, 270, 312, 794, 857
BveI ACCTGC 2 cut(s) 113, 162
Cac8I GCNNGC 8 cut(s) 461, 1006, 1178, 1248, 1305, 1803, 1807, 1897
Cfr10I RCCGGY 1 cut(s) 741
Cfr13I GGNCC 2 cut(s) 1547, 1800
Csp6I GTAC 8 cut(s) 175, 190, 727, 915, 935, 1033, 1555, 1986
CspAI ACCGGT 1 cut(s) 741
CspCI CAANNNNNGTGG 1 cut(s) 1977
CviAII CATG 6 cut(s) 10, 633, 918, 1128, 1738, 1806
CviQI GTAC 8 cut(s) 175, 190, 727, 915, 935, 1033, 1555, 1986
DdeI CTNAG 6 cut(s) 50, 204, 609, 705, 840, 1388
DpnI GATC 7 cut(s) 276, 537, 849, 1266, 1319, 1773, 1971
DpnII GATC 7 cut(s) 274, 535, 847, 1264, 1317, 1771, 1969
DraI TTTAAA 2 cut(s) 264, 1045
Eam1104I CTCTTC 1 cut(s) 1373
EarI CTCTTC 1 cut(s) 1373
Eco32I GATATC 1 cut(s) 659
Eco47I GGWCC 1 cut(s) 1547
Eco57I CTGAAG 1 cut(s) 1649
Eco88I CYCGRG 1 cut(s) 1833
EcoNI CCTNNNNNAGG 1 cut(s) 543
EcoRI GAATTC 2 cut(s) 1211, 1311
EcoRII CCWGG 3 cut(s) 154, 1067, 1101
EcoRV GATATC 1 cut(s) 659
FaeI CATG 6 cut(s) 13, 636, 921, 1131, 1741, 1809
FaqI GGGAC 1 cut(s) 643
FatI CATG 6 cut(s) 9, 632, 917, 1127, 1737, 1805
FauI CCCGC 1 cut(s) 1810
FauNDI CATATG 2 cut(s) 775, 1138
FbaI TGATCA 3 cut(s) 274, 1771, 1969
Fnu4HI GCNGC 4 cut(s) 1009, 1174, 1251, 1519
FokI GGATG 4 cut(s) 310, 577, 722, 993
Fsp4HI GCNGC 4 cut(s) 1009, 1174, 1251, 1519
FspBI CTAG 7 cut(s) 621, 885, 1262, 1304, 1724, 1863, 1896
GluI GCNGC 4 cut(s) 1009, 1174, 1251, 1519
GsaI CCCAGC 1 cut(s) 1532
HaeIII GGCC 1 cut(s) 1801
HapII CCGG 1 cut(s) 742
Hin1II CATG 6 cut(s) 13, 636, 921, 1131, 1741, 1809
HincII GTYRAC 1 cut(s) 702
HindII GTYRAC 1 cut(s) 702
HindIII AAGCTT 3 cut(s) 473, 1685, 1750
HpaII CCGG 1 cut(s) 742
HphI GGTGA 3 cut(s) 1207, 1544, 1979
Hpy166II GTNNAC 4 cut(s) 192, 702, 729, 1555
Hpy188I TCNGA 7 cut(s) 948, 1222, 1258, 1317, 1391, 1674, 1939
Hpy188III TCNNGA 9 cut(s) 336, 413, 989, 1229, 1614, 1706, 1775, 1856, 1863
Hpy8I GTNNAC 4 cut(s) 192, 702, 729, 1555
HpyAV CCTTC 9 cut(s) 55, 302, 549, 595, 920, 1154, 1419, 1529, 1885
HpyCH4III ACNGT 6 cut(s) 211, 316, 789, 1124, 1439, 1985
HpyCH4IV ACGT 1 cut(s) 814
HpyF10VI GCNNNNNNNGC 3 cut(s) 309, 1290, 1798
HpyF3I CTNAG 6 cut(s) 50, 204, 609, 705, 840, 1388
HpySE526I ACGT 1 cut(s) 814
Hsp92II CATG 6 cut(s) 13, 636, 921, 1131, 1741, 1809
Ksp22I TGATCA 3 cut(s) 274, 1771, 1969
Kzo9I GATC 7 cut(s) 274, 535, 847, 1264, 1317, 1771, 1969
LmnI GCTCC 3 cut(s) 525, 605, 1400
Lsp1109I GCAGC 4 cut(s) 1020, 1160, 1237, 1530
LweI GCATC 2 cut(s) 1262, 1678
MaeI CTAG 7 cut(s) 621, 885, 1262, 1304, 1724, 1863, 1896
MaeII ACGT 1 cut(s) 814
MaeIII GTNAC 4 cut(s) 268, 490, 899, 1464
MalI GATC 7 cut(s) 276, 537, 849, 1266, 1319, 1773, 1971
MboI GATC 7 cut(s) 274, 535, 847, 1264, 1317, 1771, 1969
MboII GAAGA 9 cut(s) 545, 1056, 1390, 1419, 1457, 1466, 1721, 1727, 1991
MflI RGATCY 2 cut(s) 535, 1264
MhlI GDGCHC 1 cut(s) 530
MlyI GAGTC 3 cut(s) 65, 988, 1853
MmeI TCCRAC 3 cut(s) 107, 461, 625
MroXI GAANNNNTTC 2 cut(s) 1123, 1719
MseI TTAA 6 cut(s) 96, 263, 650, 767, 1044, 1868
MspI CCGG 1 cut(s) 742
MspR9I CCNGG 3 cut(s) 156, 1069, 1103
Mva1269I GAATGC 2 cut(s) 1350, 1701
MvaI CCWGG 3 cut(s) 156, 1069, 1103
MwoI GCNNNNNNNGC 3 cut(s) 309, 1290, 1798
NdeI CATATG 2 cut(s) 775, 1138
NdeII GATC 7 cut(s) 274, 535, 847, 1264, 1317, 1771, 1969
NheI GCTAGC 2 cut(s) 1303, 1895
NlaIII CATG 6 cut(s) 13, 636, 921, 1131, 1741, 1809
NlaIV GGNNCC 3 cut(s) 825, 1193, 1402
NmuCI GTSAC 1 cut(s) 268
NspI RCATGY 3 cut(s) 921, 1741, 1809
PaeI GCATGC 1 cut(s) 1809
PaeR7I CTCGAG 1 cut(s) 1833
PaqCI CACCTGC 1 cut(s) 113
PctI GAATGC 2 cut(s) 1350, 1701
PdmI GAANNNNTTC 2 cut(s) 1123, 1719
PfeI GAWTC 8 cut(s) 144, 248, 339, 349, 371, 379, 1223, 1675
PinAI ACCGGT 1 cut(s) 741
PkrI GCNGC 4 cut(s) 1010, 1175, 1252, 1520
PleI GAGTC 3 cut(s) 65, 988, 1853
PpsI GAGTC 3 cut(s) 65, 988, 1853
Ppu21I YACGTR 1 cut(s) 815
PshAI GACNNNNGTC 1 cut(s) 1944
Psp6I CCWGG 3 cut(s) 154, 1067, 1101
PspFI CCCAGC 1 cut(s) 1528
PspGI CCWGG 3 cut(s) 154, 1067, 1101
PspN4I GGNNCC 3 cut(s) 825, 1193, 1402
PspPI GGNCC 2 cut(s) 1547, 1800
PspXI VCTCGAGB 1 cut(s) 1833
PsrI GAACNNNNNNTAC 2 cut(s) 46, 78
PsuI RGATCY 2 cut(s) 535, 1264
RsaI GTAC 8 cut(s) 176, 191, 728, 916, 936, 1034, 1556, 1987
RsaNI GTAC 8 cut(s) 175, 190, 727, 915, 935, 1033, 1555, 1986
SaqAI TTAA 6 cut(s) 96, 263, 650, 767, 1044, 1868
SatI GCNGC 4 cut(s) 1009, 1174, 1251, 1519
Sau3AI GATC 7 cut(s) 274, 535, 847, 1264, 1317, 1771, 1969
Sau96I GGNCC 2 cut(s) 1547, 1800
SchI GAGTC 3 cut(s) 65, 988, 1853
ScrFI CCNGG 3 cut(s) 156, 1069, 1103
SduI GDGCHC 1 cut(s) 530
SfaNI GCATC 2 cut(s) 1262, 1678
Sfr274I CTCGAG 1 cut(s) 1833
SinI GGWCC 1 cut(s) 1547
SlaI CTCGAG 1 cut(s) 1833
SmlI CTYRAG 4 cut(s) 543, 1091, 1227, 1833
SmoI CTYRAG 4 cut(s) 543, 1091, 1227, 1833
SphI GCATGC 1 cut(s) 1809
SsiI CCGC 2 cut(s) 683, 1803
SspMI CTAG 7 cut(s) 621, 885, 1262, 1304, 1724, 1863, 1896
StyD4I CCNGG 3 cut(s) 154, 1067, 1101
TaaI ACNGT 6 cut(s) 211, 316, 789, 1124, 1439, 1985
TaiI ACGT 1 cut(s) 817
TaqI TCGA 1 cut(s) 1834
TaqII GACCGA 1 cut(s) 1934
TatI WGTACW 5 cut(s) 726, 914, 1032, 1554, 1985
TfiI GAWTC 8 cut(s) 144, 248, 339, 349, 371, 379, 1223, 1675
Tru1I TTAA 6 cut(s) 96, 263, 650, 767, 1044, 1868
Tru9I TTAA 6 cut(s) 96, 263, 650, 767, 1044, 1868
TscAI CASTG 5 cut(s) 72, 277, 319, 794, 857
TseFI GTSAC 1 cut(s) 268
TseI GCWGC 4 cut(s) 1008, 1173, 1250, 1518
Tsp45I GTSAC 1 cut(s) 268
TspGWI ACGGA 2 cut(s) 577, 1390
TspRI CASTG 5 cut(s) 72, 277, 319, 794, 857
VpaK11BI GGWCC 1 cut(s) 1547
XagI CCTNNNNNAGG 1 cut(s) 543
XapI RAATTY 6 cut(s) 484, 1144, 1211, 1311, 1609, 1719
XbaI TCTAGA 1 cut(s) 1862
XceI RCATGY 3 cut(s) 921, 1741, 1809
XcmI CCANNNNNNNNNTGG 1 cut(s) 1411
XhoI CTCGAG 1 cut(s) 1833
XmnI GAANNNNTTC 2 cut(s) 1123, 1719
XspI CTAG 7 cut(s) 621, 885, 1262, 1304, 1724, 1863, 1896
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.