Serine threonine-protein kinase
| Domain Name | Pfam ID | Position | E-value | Description |
|---|---|---|---|---|
| B_lectin | PF01453 | 2 - 54 | 2.8e-17 | D-mannose binding lectin |
| S_locus_glycop | PF00954 | 88 - 198 | 3.6e-28 | S-locus glycoprotein domain |
| PAN_2 | PF08276 | 219 - 287 | 1.3e-19 | PAN-like domain |
| Pkinase | PF00069 | 381 - 582 | 7.5e-42 | Protein kinase domain |
| PK_Tyr_Ser-Thr | PF07714 | 384 - 648 | 1.6e-46 | Protein tyrosine and serine/threonine kinase |
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 2 cut(s) | 1727, 1946 |
| AasI | GACNNNNNNGTC | 1 cut(s) | 619 |
| Acc16I | TGCGCA | 1 cut(s) | 638 |
| Acc36I | ACCTGC | 1 cut(s) | 666 |
| AccB1I | GGYRCC | 1 cut(s) | 1648 |
| AccB7I | CCANNNNNTGG | 2 cut(s) | 281, 878 |
| AccI | GTMKAC | 2 cut(s) | 571, 1008 |
| AccII | CGCG | 1 cut(s) | 435 |
| AccIII | TCCGGA | 1 cut(s) | 240 |
| AclI | AACGTT | 2 cut(s) | 259, 1432 |
| AclWI | GGATC | 3 cut(s) | 894, 1751, 1926 |
| AcoI | YGGCCR | 4 cut(s) | 283, 880, 1240, 1922 |
| AcsI | RAATTY | 5 cut(s) | 163, 188, 290, 607, 1557 |
| AcuI | CTGAAG | 3 cut(s) | 894, 1079, 1274 |
| AfaI | GTAC | 5 cut(s) | 6, 365, 374, 489, 1074 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 182, 281, 878, 1494, 2030 |
| AflIII | ACRYGT | 1 cut(s) | 369 |
| AgsI | TTSAA | 9 cut(s) | 206, 402, 455, 700, 772, 1030, 1105, 1230, 1883 |
| AjnI | CCWGG | 7 cut(s) | 181, 657, 882, 1086, 1374, 1487, 1987 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 1155, 1187 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 764, 796 |
| Alw21I | GWGCWC | 3 cut(s) | 1095, 1115, 1850 |
| Alw26I | GTCTC | 2 cut(s) | 1028, 1469 |
| AlwI | GGATC | 3 cut(s) | 894, 1751, 1926 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 878 |
| Ama87I | CYCGRG | 1 cut(s) | 1458 |
| Aor13HI | TCCGGA | 1 cut(s) | 240 |
| AoxI | GGCC | 8 cut(s) | 271, 283, 314, 880, 1240, 1424, 1814, 1922 |
| ApeKI | GCWGC | 3 cut(s) | 654, 828, 1661 |
| ApoI | RAATTY | 5 cut(s) | 163, 188, 290, 607, 1557 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 41, 73 |
| AsiSI | GCGATCGC | 1 cut(s) | 439 |
| AspLEI | GCGC | 2 cut(s) | 639, 833 |
| AspS9I | GGNCC | 4 cut(s) | 272, 1020, 1084, 1175 |
| AsuHPI | GGTGA | 3 cut(s) | 585, 857, 932 |
| AsuII | TTCGAA | 3 cut(s) | 382, 898, 2061 |
| AvaI | CYCGRG | 1 cut(s) | 1458 |
| AvaII | GGWCC | 3 cut(s) | 1020, 1084, 1175 |
| AxyI | CCTNAGG | 2 cut(s) | 228, 1914 |
| BalI | TGGCCA | 3 cut(s) | 285, 882, 1242 |
| BanI | GGYRCC | 1 cut(s) | 1648 |
| BanII | GRGCYC | 2 cut(s) | 1095, 1850 |
| BauI | CACGAG | 1 cut(s) | 1122 |
| BbsI | GAAGAC | 2 cut(s) | 177, 944 |
| Bbv12I | GWGCWC | 3 cut(s) | 1095, 1115, 1850 |
| BbvI | GCAGC | 3 cut(s) | 641, 815, 1648 |
| BccI | CCATC | 4 cut(s) | 106, 111, 920, 1661 |
| BciT130I | CCWGG | 7 cut(s) | 183, 659, 884, 1088, 1376, 1489, 1989 |
| BclI | TGATCA | 1 cut(s) | 1393 |
| BcoDI | GTCTC | 2 cut(s) | 1028, 1469 |
| BfaI | CTAG | 4 cut(s) | 29, 492, 854, 1578 |
| BfuAI | ACCTGC | 1 cut(s) | 666 |
| BglII | AGATCT | 2 cut(s) | 496, 1510 |
| BisI | GCNGC | 3 cut(s) | 655, 829, 1662 |
| BlsI | GCNGC | 3 cut(s) | 656, 830, 1663 |
| Bme1390I | CCNGG | 7 cut(s) | 183, 659, 884, 1088, 1376, 1489, 1989 |
| Bme18I | GGWCC | 3 cut(s) | 1020, 1084, 1175 |
| BmeT110I | CYCGRG | 1 cut(s) | 1458 |
| BmgT120I | GGNCC | 4 cut(s) | 272, 1020, 1084, 1175 |
| BmiI | GGNNCC | 2 cut(s) | 421, 1650 |
| BmrFI | CCNGG | 7 cut(s) | 183, 659, 884, 1088, 1376, 1489, 1989 |
| BmsI | GCATC | 5 cut(s) | 311, 491, 700, 865, 1943 |
| BoxI | GACNNNNGTC | 1 cut(s) | 177 |
| BpiI | GAAGAC | 2 cut(s) | 177, 944 |
| BpmI | CTGGAG | 3 cut(s) | 1109, 1626, 1739 |
| Bpu10I | CCTNAGC | 2 cut(s) | 650, 1493 |
| Bpu14I | TTCGAA | 3 cut(s) | 382, 898, 2061 |
| Bsa29I | ATCGAT | 1 cut(s) | 508 |
| BsaI | GGTCTC | 1 cut(s) | 1469 |
| BsaJI | CCNNGG | 3 cut(s) | 182, 275, 1243 |
| BsaWI | WCCGGW | 3 cut(s) | 100, 240, 1589 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 182, 281, 878, 1494, 2030 |
| Bse1I | ACTGG | 3 cut(s) | 557, 623, 1609 |
| Bse21I | CCTNAGG | 2 cut(s) | 228, 1914 |
| BseAI | TCCGGA | 1 cut(s) | 240 |
| BseBI | CCWGG | 7 cut(s) | 183, 659, 884, 1088, 1376, 1489, 1989 |
| BseCI | ATCGAT | 1 cut(s) | 508 |
| BseDI | CCNNGG | 3 cut(s) | 182, 275, 1243 |
| BseGI | GGATG | 4 cut(s) | 122, 635, 715, 1909 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 182, 281, 878, 1494, 2030 |
| BseMII | CTCAG | 2 cut(s) | 219, 1905 |
| BseNI | ACTGG | 3 cut(s) | 557, 623, 1609 |
| BseRI | GAGGAG | 2 cut(s) | 1457, 1608 |
| BseXI | GCAGC | 3 cut(s) | 641, 815, 1648 |
| Bsh1236I | CGCG | 1 cut(s) | 435 |
| Bsh1285I | CGRYCG | 1 cut(s) | 439 |
| BshFI | GGCC | 8 cut(s) | 273, 285, 316, 882, 1242, 1426, 1816, 1924 |
| BshNI | GGYRCC | 1 cut(s) | 1648 |
| BshVI | ATCGAT | 1 cut(s) | 508 |
| BsiEI | CGRYCG | 1 cut(s) | 439 |
| BsiHKAI | GWGCWC | 3 cut(s) | 1095, 1115, 1850 |
| BsiHKCI | CYCGRG | 1 cut(s) | 1458 |
| BsiSI | CCGG | 4 cut(s) | 101, 241, 707, 1590 |
| BslFI | GGGAC | 3 cut(s) | 134, 1204, 1645 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 182, 281, 878, 1494, 2030 |
| BsmAI | GTCTC | 2 cut(s) | 1028, 1469 |
| BsmFI | GGGAC | 3 cut(s) | 134, 1204, 1645 |
| BsmI | GAATGC | 3 cut(s) | 758, 990, 1878 |
| BsnI | GGCC | 8 cut(s) | 273, 285, 316, 882, 1242, 1426, 1816, 1924 |
| Bso31I | GGTCTC | 1 cut(s) | 1469 |
| BsoBI | CYCGRG | 1 cut(s) | 1458 |
| Bsp119I | TTCGAA | 3 cut(s) | 382, 898, 2061 |
| Bsp1286I | GDGCHC | 3 cut(s) | 1095, 1115, 1850 |
| Bsp13I | TCCGGA | 1 cut(s) | 240 |
| Bsp1407I | TGTACA | 1 cut(s) | 372 |
| Bsp19I | CCATGG | 1 cut(s) | 275 |
| BspANI | GGCC | 8 cut(s) | 273, 285, 316, 882, 1242, 1426, 1816, 1924 |
| BspCNI | CTCAG | 2 cut(s) | 220, 1906 |
| BspDI | ATCGAT | 1 cut(s) | 508 |
| BspEI | TCCGGA | 1 cut(s) | 240 |
| BspFNI | CGCG | 1 cut(s) | 435 |
| BspHI | TCATGA | 1 cut(s) | 1260 |
| BspLI | GGNNCC | 2 cut(s) | 421, 1650 |
| BspMI | ACCTGC | 1 cut(s) | 666 |
| BspPI | GGATC | 3 cut(s) | 894, 1751, 1926 |
| BspT104I | TTCGAA | 3 cut(s) | 382, 898, 2061 |
| BspT107I | GGYRCC | 1 cut(s) | 1648 |
| BspTNI | GGTCTC | 1 cut(s) | 1469 |
| BsrGI | TGTACA | 1 cut(s) | 372 |
| BsrI | ACTGG | 3 cut(s) | 557, 623, 1609 |
| BssECI | CCNNGG | 3 cut(s) | 182, 275, 1243 |
| BssSI | CACGAG | 1 cut(s) | 1122 |
| BssT1I | CCWWGG | 2 cut(s) | 275, 1243 |
| Bst2BI | CACGAG | 1 cut(s) | 1122 |
| Bst2UI | CCWGG | 7 cut(s) | 183, 659, 884, 1088, 1376, 1489, 1989 |
| Bst4CI | ACNGT | 3 cut(s) | 554, 779, 1416 |
| Bst6I | CTCTTC | 2 cut(s) | 1214, 1329 |
| BstAUI | TGTACA | 1 cut(s) | 372 |
| BstBI | TTCGAA | 3 cut(s) | 382, 898, 2061 |
| BstC8I | GCNNGC | 4 cut(s) | 880, 1328, 1901, 2020 |
| BstDEI | CTNAG | 8 cut(s) | 228, 311, 588, 650, 991, 1493, 1617, 1914 |
| BstDSI | CCRYGG | 1 cut(s) | 275 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 2028 |
| BstF5I | GGATG | 4 cut(s) | 122, 635, 715, 1909 |
| BstFNI | CGCG | 1 cut(s) | 435 |
| BstHHI | GCGC | 2 cut(s) | 639, 833 |
| BstMAI | GTCTC | 2 cut(s) | 1028, 1469 |
| BstMCI | CGRYCG | 1 cut(s) | 439 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 1239 |
| BstNI | CCWGG | 7 cut(s) | 183, 659, 884, 1088, 1376, 1489, 1989 |
| BstNSI | RCATGY | 2 cut(s) | 373, 1363 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 177 |
| BstSCI | CCNGG | 7 cut(s) | 181, 657, 882, 1086, 1374, 1487, 1987 |
| BstUI | CGCG | 1 cut(s) | 435 |
| BstV1I | GCAGC | 3 cut(s) | 641, 815, 1648 |
| BstV2I | GAAGAC | 2 cut(s) | 177, 944 |
| BstX2I | RGATCY | 3 cut(s) | 496, 886, 1510 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 603 |
| BstYI | RGATCY | 3 cut(s) | 496, 886, 1510 |
| Bsu15I | ATCGAT | 1 cut(s) | 508 |
| Bsu36I | CCTNAGG | 2 cut(s) | 228, 1914 |
| BsuRI | GGCC | 8 cut(s) | 273, 285, 316, 882, 1242, 1426, 1816, 1924 |
| BsuTUI | ATCGAT | 1 cut(s) | 508 |
| BtgI | CCRYGG | 1 cut(s) | 275 |
| BtsCI | GGATG | 4 cut(s) | 122, 635, 715, 1909 |
| BtsIMutI | CAGTG | 1 cut(s) | 301 |
| BveI | ACCTGC | 1 cut(s) | 666 |
| Cac8I | GCNNGC | 4 cut(s) | 880, 1328, 1901, 2020 |
| CaiI | CAGNNNCTG | 1 cut(s) | 878 |
| CciI | TCATGA | 1 cut(s) | 1260 |
| CfoI | GCGC | 2 cut(s) | 639, 833 |
| Cfr13I | GGNCC | 4 cut(s) | 272, 1020, 1084, 1175 |
| ClaI | ATCGAT | 1 cut(s) | 508 |
| Csp6I | GTAC | 5 cut(s) | 5, 364, 373, 488, 1073 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 1427, 1462 |
| CviQI | GTAC | 5 cut(s) | 5, 364, 373, 488, 1073 |
| DdeI | CTNAG | 8 cut(s) | 228, 311, 588, 650, 991, 1493, 1617, 1914 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 619 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 619 |
| EaeI | YGGCCR | 4 cut(s) | 283, 880, 1240, 1922 |
| Eam1104I | CTCTTC | 2 cut(s) | 1214, 1329 |
| EarI | CTCTTC | 2 cut(s) | 1214, 1329 |
| Ecl136II | GAGCTC | 2 cut(s) | 1093, 1848 |
| Eco130I | CCWWGG | 2 cut(s) | 275, 1243 |
| Eco147I | AGGCCT | 2 cut(s) | 316, 1816 |
| Eco24I | GRGCYC | 2 cut(s) | 1095, 1850 |
| Eco31I | GGTCTC | 1 cut(s) | 1469 |
| Eco32I | GATATC | 2 cut(s) | 808, 859 |
| Eco47I | GGWCC | 3 cut(s) | 1020, 1084, 1175 |
| Eco53kI | GAGCTC | 2 cut(s) | 1093, 1848 |
| Eco57I | CTGAAG | 3 cut(s) | 894, 1079, 1274 |
| Eco81I | CCTNAGG | 2 cut(s) | 228, 1914 |
| Eco88I | CYCGRG | 1 cut(s) | 1458 |
| EcoICRI | GAGCTC | 2 cut(s) | 1093, 1848 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 2028 |
| EcoO109I | RGGNCCY | 1 cut(s) | 1084 |
| EcoRI | GAATTC | 3 cut(s) | 188, 290, 607 |
| EcoRII | CCWGG | 7 cut(s) | 181, 657, 882, 1086, 1374, 1487, 1987 |
| EcoRV | GATATC | 2 cut(s) | 808, 859 |
| EcoT14I | CCWWGG | 2 cut(s) | 275, 1243 |
| EcoT22I | ATGCAT | 2 cut(s) | 223, 484 |
| EcoT38I | GRGCYC | 2 cut(s) | 1095, 1850 |
| ErhI | CCWWGG | 2 cut(s) | 275, 1243 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 1875, 1907 |
| FaqI | GGGAC | 3 cut(s) | 134, 1204, 1645 |
| FauNDI | CATATG | 1 cut(s) | 1636 |
| FbaI | TGATCA | 1 cut(s) | 1393 |
| FblI | GTMKAC | 2 cut(s) | 571, 1008 |
| Fnu4HI | GCNGC | 3 cut(s) | 655, 829, 1662 |
| FokI | GGATG | 4 cut(s) | 129, 642, 722, 1896 |
| FriOI | GRGCYC | 2 cut(s) | 1095, 1850 |
| Fsp4HI | GCNGC | 3 cut(s) | 655, 829, 1662 |
| FspBI | CTAG | 4 cut(s) | 29, 492, 854, 1578 |
| FspI | TGCGCA | 1 cut(s) | 638 |
| GlaI | GCGC | 2 cut(s) | 638, 832 |
| GluI | GCNGC | 3 cut(s) | 655, 829, 1662 |
| GsuI | CTGGAG | 3 cut(s) | 1109, 1626, 1739 |
| HaeIII | GGCC | 8 cut(s) | 273, 285, 316, 882, 1242, 1426, 1816, 1924 |
| HapII | CCGG | 4 cut(s) | 101, 241, 707, 1590 |
| HhaI | GCGC | 2 cut(s) | 639, 833 |
| Hin6I | GCGC | 2 cut(s) | 637, 831 |
| HinP1I | GCGC | 2 cut(s) | 637, 831 |
| HindIII | AAGCTT | 4 cut(s) | 70, 799, 866, 1154 |
| HinfI | GANTC | 7 cut(s) | 17, 579, 1312, 1484, 1549, 1832, 2039 |
| HpaII | CCGG | 4 cut(s) | 101, 241, 707, 1590 |
| HphI | GGTGA | 3 cut(s) | 585, 857, 932 |
| Hpy166II | GTNNAC | 6 cut(s) | 364, 572, 730, 1009, 1183, 1733 |
| Hpy188III | TCNNGA | 9 cut(s) | 21, 241, 872, 981, 1096, 1261, 1630, 1760, 1913 |
| Hpy8I | GTNNAC | 6 cut(s) | 364, 572, 730, 1009, 1183, 1733 |
| HpyAV | CCTTC | 4 cut(s) | 93, 1157, 1802, 1806 |
| HpyCH4III | ACNGT | 3 cut(s) | 554, 779, 1416 |
| HpyCH4IV | ACGT | 4 cut(s) | 259, 1005, 1432, 1873 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 1239 |
| HpyF3I | CTNAG | 8 cut(s) | 228, 311, 588, 650, 991, 1493, 1617, 1914 |
| HpySE526I | ACGT | 4 cut(s) | 259, 1005, 1432, 1873 |
| HspAI | GCGC | 2 cut(s) | 637, 831 |
| Kpn2I | TCCGGA | 1 cut(s) | 240 |
| Ksp22I | TGATCA | 1 cut(s) | 1393 |
| LmnI | GCTCC | 3 cut(s) | 524, 1090, 1098 |
| Lsp1109I | GCAGC | 3 cut(s) | 641, 815, 1648 |
| LweI | GCATC | 5 cut(s) | 311, 491, 700, 865, 1943 |
| MaeI | CTAG | 4 cut(s) | 29, 492, 854, 1578 |
| MaeII | ACGT | 4 cut(s) | 259, 1005, 1432, 1873 |
| MaeIII | GTNAC | 6 cut(s) | 43, 96, 548, 591, 621, 1869 |
| MflI | RGATCY | 3 cut(s) | 496, 886, 1510 |
| MhlI | GDGCHC | 3 cut(s) | 1095, 1115, 1850 |
| MlsI | TGGCCA | 3 cut(s) | 285, 882, 1242 |
| MluNI | TGGCCA | 3 cut(s) | 285, 882, 1242 |
| Mox20I | TGGCCA | 3 cut(s) | 285, 882, 1242 |
| Mph1103I | ATGCAT | 2 cut(s) | 223, 484 |
| MroI | TCCGGA | 1 cut(s) | 240 |
| MscI | TGGCCA | 3 cut(s) | 285, 882, 1242 |
| MseI | TTAA | 5 cut(s) | 138, 413, 1251, 1272, 1586 |
| MslI | CAYNNNNRTG | 2 cut(s) | 628, 899 |
| Msp20I | TGGCCA | 3 cut(s) | 285, 882, 1242 |
| MspI | CCGG | 4 cut(s) | 101, 241, 707, 1590 |
| MspR9I | CCNGG | 7 cut(s) | 183, 659, 884, 1088, 1376, 1489, 1989 |
| Mva1269I | GAATGC | 3 cut(s) | 758, 990, 1878 |
| MvaI | CCWGG | 7 cut(s) | 183, 659, 884, 1088, 1376, 1489, 1989 |
| MvnI | CGCG | 1 cut(s) | 435 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 1239 |
| NcoI | CCATGG | 1 cut(s) | 275 |
| NdeI | CATATG | 1 cut(s) | 1636 |
| NlaIV | GGNNCC | 2 cut(s) | 421, 1650 |
| NmuCI | GTSAC | 4 cut(s) | 43, 548, 591, 621 |
| NsbI | TGCGCA | 1 cut(s) | 638 |
| NsiI | ATGCAT | 2 cut(s) | 223, 484 |
| NspI | RCATGY | 2 cut(s) | 373, 1363 |
| NspV | TTCGAA | 3 cut(s) | 382, 898, 2061 |
| PagI | TCATGA | 1 cut(s) | 1260 |
| PceI | AGGCCT | 2 cut(s) | 316, 1816 |
| PciI | ACATGT | 1 cut(s) | 369 |
| PctI | GAATGC | 3 cut(s) | 758, 990, 1878 |
| PfeI | GAWTC | 7 cut(s) | 17, 579, 1312, 1484, 1549, 1832, 2039 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 281, 878 |
| PkrI | GCNGC | 3 cut(s) | 656, 830, 1663 |
| Ple19I | CGATCG | 1 cut(s) | 439 |
| PpuMI | RGGWCCY | 1 cut(s) | 1084 |
| PscI | ACATGT | 1 cut(s) | 369 |
| PshAI | GACNNNNGTC | 1 cut(s) | 177 |
| PsiI | TTATAA | 2 cut(s) | 1727, 1946 |
| Psp124BI | GAGCTC | 2 cut(s) | 1095, 1850 |
| Psp1406I | AACGTT | 2 cut(s) | 259, 1432 |
| Psp5II | RGGWCCY | 1 cut(s) | 1084 |
| Psp6I | CCWGG | 7 cut(s) | 181, 657, 882, 1086, 1374, 1487, 1987 |
| PspGI | CCWGG | 7 cut(s) | 181, 657, 882, 1086, 1374, 1487, 1987 |
| PspN4I | GGNNCC | 2 cut(s) | 421, 1650 |
| PspPI | GGNCC | 4 cut(s) | 272, 1020, 1084, 1175 |
| PspPPI | RGGWCCY | 1 cut(s) | 1084 |
| PstNI | CAGNNNCTG | 1 cut(s) | 878 |
| PsuI | RGATCY | 3 cut(s) | 496, 886, 1510 |
| PvuI | CGATCG | 1 cut(s) | 439 |
| RgaI | GCGATCGC | 1 cut(s) | 439 |
| RsaI | GTAC | 5 cut(s) | 6, 365, 374, 489, 1074 |
| RsaNI | GTAC | 5 cut(s) | 5, 364, 373, 488, 1073 |
| RseI | CAYNNNNRTG | 2 cut(s) | 628, 899 |
| SacI | GAGCTC | 2 cut(s) | 1095, 1850 |
| SaqAI | TTAA | 5 cut(s) | 138, 413, 1251, 1272, 1586 |
| SatI | GCNGC | 3 cut(s) | 655, 829, 1662 |
| Sau96I | GGNCC | 4 cut(s) | 272, 1020, 1084, 1175 |
| ScrFI | CCNGG | 7 cut(s) | 183, 659, 884, 1088, 1376, 1489, 1989 |
| SduI | GDGCHC | 3 cut(s) | 1095, 1115, 1850 |
| SfaAI | GCGATCGC | 1 cut(s) | 439 |
| SfaNI | GCATC | 5 cut(s) | 311, 491, 700, 865, 1943 |
| SfuI | TTCGAA | 3 cut(s) | 382, 898, 2061 |
| SgfI | GCGATCGC | 1 cut(s) | 439 |
| SinI | GGWCC | 3 cut(s) | 1020, 1084, 1175 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 628, 899 |
| SseBI | AGGCCT | 2 cut(s) | 316, 1816 |
| SspI | AATATT | 1 cut(s) | 1584 |
| SspMI | CTAG | 4 cut(s) | 29, 492, 854, 1578 |
| SstI | GAGCTC | 2 cut(s) | 1095, 1850 |
| StuI | AGGCCT | 2 cut(s) | 316, 1816 |
| StyD4I | CCNGG | 7 cut(s) | 181, 657, 882, 1086, 1374, 1487, 1987 |
| StyI | CCWWGG | 2 cut(s) | 275, 1243 |
| TaaI | ACNGT | 3 cut(s) | 554, 779, 1416 |
| TaiI | ACGT | 4 cut(s) | 262, 1008, 1435, 1876 |
| TaqI | TCGA | 8 cut(s) | 75, 336, 382, 508, 565, 611, 898, 2061 |
| TatI | WGTACW | 2 cut(s) | 363, 372 |
| TfiI | GAWTC | 7 cut(s) | 17, 579, 1312, 1484, 1549, 1832, 2039 |
| Tru1I | TTAA | 5 cut(s) | 138, 413, 1251, 1272, 1586 |
| Tru9I | TTAA | 5 cut(s) | 138, 413, 1251, 1272, 1586 |
| TscAI | CASTG | 1 cut(s) | 301 |
| TseFI | GTSAC | 4 cut(s) | 43, 548, 591, 621 |
| TseI | GCWGC | 3 cut(s) | 654, 828, 1661 |
| Tsp45I | GTSAC | 4 cut(s) | 43, 548, 591, 621 |
| TspGWI | ACGGA | 1 cut(s) | 1750 |
| TspRI | CASTG | 1 cut(s) | 301 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 281, 878 |
| VpaK11BI | GGWCC | 3 cut(s) | 1020, 1084, 1175 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 2028 |
| XapI | RAATTY | 5 cut(s) | 163, 188, 290, 607, 1557 |
| XceI | RCATGY | 2 cut(s) | 373, 1363 |
| XcmI | CCANNNNNNNNNTGG | 4 cut(s) | 293, 1372, 1445, 1778 |
| XmiI | GTMKAC | 2 cut(s) | 571, 1008 |
| XspI | CTAG | 4 cut(s) | 29, 492, 854, 1578 |
| Zsp2I | ATGCAT | 2 cut(s) | 223, 484 |