Protein kinase; unclassified specificity.
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 401 |
| Acc16I | TGCGCA | 1 cut(s) | 1412 |
| Acc36I | ACCTGC | 1 cut(s) | 1884 |
| AccB1I | GGYRCC | 2 cut(s) | 103, 2422 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 1055 |
| AccBSI | CCGCTC | 1 cut(s) | 1867 |
| AccI | GTMKAC | 1 cut(s) | 308 |
| AccII | CGCG | 2 cut(s) | 292, 1209 |
| AciI | CCGC | 2 cut(s) | 1865, 2223 |
| AclI | AACGTT | 1 cut(s) | 2206 |
| AclWI | GGATC | 2 cut(s) | 1668, 1860 |
| AcoI | YGGCCR | 1 cut(s) | 2011 |
| AcsI | RAATTY | 8 cut(s) | 12, 606, 805, 937, 1381, 1460, 1688, 2331 |
| AcuI | CTGAAG | 2 cut(s) | 556, 1668 |
| AfaI | GTAC | 8 cut(s) | 614, 813, 1139, 1148, 1204, 1263, 1597, 2629 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 837, 956, 1055, 1953, 2268 |
| AflIII | ACRYGT | 3 cut(s) | 290, 1143, 2609 |
| AjnI | CCWGG | 7 cut(s) | 442, 874, 955, 1431, 2148, 2261, 2761 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 584, 616, 1923, 1955 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 1538, 1570 |
| Alw21I | GWGCWC | 1 cut(s) | 1863 |
| Alw26I | GTCTC | 5 cut(s) | 1263, 1371, 1401, 2243, 2669 |
| AlwI | GGATC | 2 cut(s) | 1668, 1860 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 1652, 1663 |
| Ama87I | CYCGRG | 2 cut(s) | 2232, 2687 |
| ApeKI | GCWGC | 3 cut(s) | 1428, 1602, 2094 |
| ApoI | RAATTY | 8 cut(s) | 12, 606, 805, 937, 1381, 1460, 1688, 2331 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 132, 164 |
| AseI | ATTAAT | 2 cut(s) | 669, 2360 |
| AsiSI | GCGATCGC | 1 cut(s) | 1213 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 2027 |
| AspLEI | GCGC | 2 cut(s) | 1413, 1607 |
| AspS9I | GGNCC | 3 cut(s) | 1046, 1943, 2857 |
| AsuC2I | CCSGG | 1 cut(s) | 579 |
| AsuHPI | GGTGA | 3 cut(s) | 1068, 1513, 1826 |
| AsuII | TTCGAA | 1 cut(s) | 1672 |
| AvaI | CYCGRG | 2 cut(s) | 2232, 2687 |
| AvaII | GGWCC | 1 cut(s) | 1943 |
| AxyI | CCTNAGG | 1 cut(s) | 369 |
| BalI | TGGCCA | 1 cut(s) | 2013 |
| BanI | GGYRCC | 2 cut(s) | 103, 2422 |
| BanII | GRGCYC | 1 cut(s) | 1863 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 292, 324 |
| BbsI | GAAGAC | 2 cut(s) | 951, 1718 |
| Bbv12I | GWGCWC | 1 cut(s) | 1863 |
| BbvI | GCAGC | 3 cut(s) | 1415, 1589, 2081 |
| BceAI | ACGGC | 1 cut(s) | 2844 |
| BciT130I | CCWGG | 7 cut(s) | 444, 876, 957, 1433, 2150, 2263, 2763 |
| BcnI | CCSGG | 1 cut(s) | 579 |
| BcoDI | GTCTC | 5 cut(s) | 1263, 1371, 1401, 2243, 2669 |
| BfaI | CTAG | 4 cut(s) | 33, 1400, 1890, 2352 |
| BfmI | CTRYAG | 1 cut(s) | 1755 |
| BfuAI | ACCTGC | 1 cut(s) | 1884 |
| BglII | AGATCT | 1 cut(s) | 2284 |
| BisI | GCNGC | 3 cut(s) | 1429, 1603, 2095 |
| BlpI | GCTNAGC | 3 cut(s) | 429, 757, 2748 |
| BlsI | GCNGC | 3 cut(s) | 1430, 1604, 2096 |
| BmcAI | AGTACT | 2 cut(s) | 813, 1204 |
| Bme1390I | CCNGG | 8 cut(s) | 444, 579, 876, 957, 1433, 2150, 2263, 2763 |
| Bme18I | GGWCC | 1 cut(s) | 1943 |
| BmeT110I | CYCGRG | 2 cut(s) | 2232, 2687 |
| BmgT120I | GGNCC | 3 cut(s) | 1046, 1943, 2857 |
| BmiI | GGNNCC | 6 cut(s) | 105, 829, 1300, 1842, 1945, 2424 |
| BmrFI | CCNGG | 8 cut(s) | 444, 579, 876, 957, 1433, 2150, 2263, 2763 |
| BmrI | ACTGGG | 2 cut(s) | 505, 620 |
| BmsI | GCATC | 9 cut(s) | 342, 398, 647, 693, 811, 1639, 2074, 2081, 2717 |
| BmuI | ACTGGG | 2 cut(s) | 505, 620 |
| BoxI | GACNNNNGTC | 1 cut(s) | 951 |
| BpiI | GAAGAC | 2 cut(s) | 951, 1718 |
| BpmI | CTGGAG | 4 cut(s) | 471, 1877, 2045, 2513 |
| Bpu10I | CCTNAGC | 2 cut(s) | 1738, 2267 |
| Bpu1102I | GCTNAGC | 3 cut(s) | 429, 757, 2748 |
| Bpu14I | TTCGAA | 1 cut(s) | 1672 |
| BpuEI | CTTGAG | 4 cut(s) | 919, 1159, 1353, 1505 |
| BpuMI | CCSGG | 1 cut(s) | 579 |
| Bsa29I | ATCGAT | 1 cut(s) | 2840 |
| BsaAI | YACGTR | 2 cut(s) | 1146, 2610 |
| BsaBI | GATNNNNATC | 2 cut(s) | 996, 1326 |
| BsaI | GGTCTC | 2 cut(s) | 1401, 2243 |
| BsaJI | CCNNGG | 6 cut(s) | 384, 423, 534, 1049, 2014, 2688 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 837, 956, 1055, 1953, 2268 |
| Bse1I | ACTGG | 7 cut(s) | 488, 511, 626, 1200, 1331, 1397, 1603 |
| Bse21I | CCTNAGG | 1 cut(s) | 369 |
| Bse3DI | GCAATG | 1 cut(s) | 1725 |
| Bse8I | GATNNNNATC | 2 cut(s) | 996, 1326 |
| BseBI | CCWGG | 7 cut(s) | 444, 876, 957, 1433, 2150, 2263, 2763 |
| BseCI | ATCGAT | 1 cut(s) | 2840 |
| BseDI | CCNNGG | 6 cut(s) | 384, 423, 534, 1049, 2014, 2688 |
| BseJI | GATNNNNATC | 2 cut(s) | 996, 1326 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 837, 956, 1055, 1953, 2268 |
| BseMI | GCAATG | 1 cut(s) | 1725 |
| BseMII | CTCAG | 6 cut(s) | 360, 443, 771, 993, 1052, 2762 |
| BseNI | ACTGG | 7 cut(s) | 488, 511, 626, 1200, 1331, 1397, 1603 |
| BseRI | GAGGAG | 8 cut(s) | 63, 1602, 1605, 1862, 2123, 2193, 2231, 2382 |
| BseXI | GCAGC | 3 cut(s) | 1415, 1589, 2081 |
| BseYI | CCCAGC | 2 cut(s) | 1652, 2725 |
| Bsh1236I | CGCG | 2 cut(s) | 292, 1209 |
| Bsh1285I | CGRYCG | 1 cut(s) | 1213 |
| BshNI | GGYRCC | 2 cut(s) | 103, 2422 |
| BshVI | ATCGAT | 1 cut(s) | 2840 |
| BsiEI | CGRYCG | 1 cut(s) | 1213 |
| BsiHKAI | GWGCWC | 1 cut(s) | 1863 |
| BsiHKCI | CYCGRG | 2 cut(s) | 2232, 2687 |
| BsiSI | CCGG | 2 cut(s) | 578, 681 |
| BslFI | GGGAC | 3 cut(s) | 489, 908, 1972 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 837, 956, 1055, 1953, 2268 |
| BsmAI | GTCTC | 5 cut(s) | 1263, 1371, 1401, 2243, 2669 |
| BsmFI | GGGAC | 3 cut(s) | 489, 908, 1972 |
| BsmI | GAATGC | 1 cut(s) | 1532 |
| Bso31I | GGTCTC | 2 cut(s) | 1401, 2243 |
| BsoBI | CYCGRG | 2 cut(s) | 2232, 2687 |
| Bsp119I | TTCGAA | 1 cut(s) | 1672 |
| Bsp1286I | GDGCHC | 1 cut(s) | 1863 |
| Bsp143I | GATC | 8 cut(s) | 52, 1210, 1234, 1621, 1660, 1852, 2284, 2371 |
| Bsp1720I | GCTNAGC | 3 cut(s) | 429, 757, 2748 |
| Bsp19I | CCATGG | 2 cut(s) | 384, 1049 |
| Bsp68I | TCGCGA | 1 cut(s) | 1209 |
| BspACI | CCGC | 2 cut(s) | 1865, 2223 |
| BspCNI | CTCAG | 6 cut(s) | 361, 442, 770, 994, 1053, 2761 |
| BspDI | ATCGAT | 1 cut(s) | 2840 |
| BspFNI | CGCG | 2 cut(s) | 292, 1209 |
| BspLI | GGNNCC | 6 cut(s) | 105, 829, 1300, 1842, 1945, 2424 |
| BspMI | ACCTGC | 1 cut(s) | 1884 |
| BspPI | GGATC | 2 cut(s) | 1668, 1860 |
| BspQI | GCTCTTC | 1 cut(s) | 2806 |
| BspT104I | TTCGAA | 1 cut(s) | 1672 |
| BspT107I | GGYRCC | 2 cut(s) | 103, 2422 |
| BspTNI | GGTCTC | 2 cut(s) | 1401, 2243 |
| BsrBI | CCGCTC | 1 cut(s) | 1867 |
| BsrDI | GCAATG | 1 cut(s) | 1725 |
| BsrI | ACTGG | 7 cut(s) | 488, 511, 626, 1200, 1331, 1397, 1603 |
| BssECI | CCNNGG | 6 cut(s) | 384, 423, 534, 1049, 2014, 2688 |
| BssMI | GATC | 8 cut(s) | 52, 1210, 1234, 1621, 1660, 1852, 2284, 2371 |
| BssNAI | GTATAC | 1 cut(s) | 309 |
| BssT1I | CCWWGG | 5 cut(s) | 384, 423, 534, 1049, 2014 |
| Bst1107I | GTATAC | 1 cut(s) | 309 |
| Bst2UI | CCWGG | 7 cut(s) | 444, 876, 957, 1433, 2150, 2263, 2763 |
| Bst6I | CTCTTC | 2 cut(s) | 1985, 2806 |
| BstBAI | YACGTR | 2 cut(s) | 1146, 2610 |
| BstBI | TTCGAA | 1 cut(s) | 1672 |
| BstC8I | GCNNGC | 3 cut(s) | 356, 478, 2675 |
| BstDEI | CTNAG | 9 cut(s) | 369, 429, 757, 815, 1002, 1061, 1738, 2267, 2748 |
| BstDSI | CCRYGG | 2 cut(s) | 384, 1049 |
| BstFNI | CGCG | 2 cut(s) | 292, 1209 |
| BstHHI | GCGC | 2 cut(s) | 1413, 1607 |
| BstKTI | GATC | 8 cut(s) | 55, 1213, 1237, 1624, 1663, 1855, 2287, 2374 |
| BstMAI | GTCTC | 5 cut(s) | 1263, 1371, 1401, 2243, 2669 |
| BstMBI | GATC | 8 cut(s) | 52, 1210, 1234, 1621, 1660, 1852, 2284, 2371 |
| BstMCI | CGRYCG | 1 cut(s) | 1213 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 767, 776 |
| BstNI | CCWGG | 7 cut(s) | 444, 876, 957, 1433, 2150, 2263, 2763 |
| BstNSI | RCATGY | 4 cut(s) | 245, 770, 2137, 2564 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 951 |
| BstSCI | CCNGG | 8 cut(s) | 442, 577, 874, 955, 1431, 2148, 2261, 2761 |
| BstSFI | CTRYAG | 1 cut(s) | 1755 |
| BstUI | CGCG | 2 cut(s) | 292, 1209 |
| BstV1I | GCAGC | 3 cut(s) | 1415, 1589, 2081 |
| BstV2I | GAAGAC | 2 cut(s) | 951, 1718 |
| BstX2I | RGATCY | 3 cut(s) | 1660, 1852, 2284 |
| BstYI | RGATCY | 3 cut(s) | 1660, 1852, 2284 |
| BstZ17I | GTATAC | 1 cut(s) | 309 |
| Bsu15I | ATCGAT | 1 cut(s) | 2840 |
| Bsu36I | CCTNAGG | 1 cut(s) | 369 |
| BsuTUI | ATCGAT | 1 cut(s) | 2840 |
| BtgI | CCRYGG | 2 cut(s) | 384, 1049 |
| BtsI | GCAGTG | 1 cut(s) | 1023 |
| BtsIMutI | CAGTG | 3 cut(s) | 1023, 1075, 1890 |
| BtuMI | TCGCGA | 1 cut(s) | 1209 |
| BveI | ACCTGC | 1 cut(s) | 1884 |
| Cac8I | GCNNGC | 3 cut(s) | 356, 478, 2675 |
| CaiI | CAGNNNCTG | 2 cut(s) | 1652, 1663 |
| CfoI | GCGC | 2 cut(s) | 1413, 1607 |
| Cfr13I | GGNCC | 3 cut(s) | 1046, 1943, 2857 |
| ClaI | ATCGAT | 1 cut(s) | 2840 |
| CseI | GACGC | 1 cut(s) | 2641 |
| CsiI | ACCWGGT | 2 cut(s) | 442, 2761 |
| Csp6I | GTAC | 8 cut(s) | 613, 812, 1138, 1147, 1203, 1262, 1596, 2628 |
| CspCI | CAANNNNNGTGG | 8 cut(s) | 60, 95, 1681, 1716, 2201, 2236, 2379, 2414 |
| CviQI | GTAC | 8 cut(s) | 613, 812, 1138, 1147, 1203, 1262, 1596, 2628 |
| DdeI | CTNAG | 9 cut(s) | 369, 429, 757, 815, 1002, 1061, 1738, 2267, 2748 |
| DpnI | GATC | 8 cut(s) | 54, 1212, 1236, 1623, 1662, 1854, 2286, 2373 |
| DpnII | GATC | 8 cut(s) | 52, 1210, 1234, 1621, 1660, 1852, 2284, 2371 |
| EaeI | YGGCCR | 1 cut(s) | 2011 |
| Eam1104I | CTCTTC | 2 cut(s) | 1985, 2806 |
| EarI | CTCTTC | 2 cut(s) | 1985, 2806 |
| Ecl136II | GAGCTC | 1 cut(s) | 1861 |
| Eco130I | CCWWGG | 5 cut(s) | 384, 423, 534, 1049, 2014 |
| Eco147I | AGGCCT | 4 cut(s) | 271, 1090, 1250, 2590 |
| Eco24I | GRGCYC | 1 cut(s) | 1863 |
| Eco31I | GGTCTC | 2 cut(s) | 1401, 2243 |
| Eco32I | GATATC | 1 cut(s) | 1582 |
| Eco47I | GGWCC | 1 cut(s) | 1943 |
| Eco53kI | GAGCTC | 1 cut(s) | 1861 |
| Eco57I | CTGAAG | 2 cut(s) | 556, 1668 |
| Eco81I | CCTNAGG | 1 cut(s) | 369 |
| Eco88I | CYCGRG | 2 cut(s) | 2232, 2687 |
| EcoICRI | GAGCTC | 1 cut(s) | 1861 |
| EcoRI | GAATTC | 1 cut(s) | 1688 |
| EcoRII | CCWGG | 7 cut(s) | 442, 874, 955, 1431, 2148, 2261, 2761 |
| EcoRV | GATATC | 1 cut(s) | 1582 |
| EcoT14I | CCWWGG | 5 cut(s) | 384, 423, 534, 1049, 2014 |
| EcoT22I | ATGCAT | 2 cut(s) | 247, 2566 |
| EcoT38I | GRGCYC | 1 cut(s) | 1863 |
| ErhI | CCWWGG | 5 cut(s) | 384, 423, 534, 1049, 2014 |
| FaqI | GGGAC | 3 cut(s) | 489, 908, 1972 |
| FauI | CCCGC | 1 cut(s) | 1872 |
| FauNDI | CATATG | 1 cut(s) | 1009 |
| FblI | GTMKAC | 1 cut(s) | 308 |
| Fnu4HI | GCNGC | 3 cut(s) | 1429, 1603, 2095 |
| FriOI | GRGCYC | 1 cut(s) | 1863 |
| Fsp4HI | GCNGC | 3 cut(s) | 1429, 1603, 2095 |
| FspBI | CTAG | 4 cut(s) | 33, 1400, 1890, 2352 |
| FspI | TGCGCA | 1 cut(s) | 1412 |
| GlaI | GCGC | 2 cut(s) | 1412, 1606 |
| GluI | GCNGC | 3 cut(s) | 1429, 1603, 2095 |
| GsaI | CCCAGC | 2 cut(s) | 1656, 2729 |
| GsuI | CTGGAG | 4 cut(s) | 471, 1877, 2045, 2513 |
| HapII | CCGG | 2 cut(s) | 578, 681 |
| HgaI | GACGC | 1 cut(s) | 2641 |
| HhaI | GCGC | 2 cut(s) | 1413, 1607 |
| Hin6I | GCGC | 2 cut(s) | 1411, 1605 |
| HinP1I | GCGC | 2 cut(s) | 1411, 1605 |
| HincII | GTYRAC | 1 cut(s) | 493 |
| HindII | GTYRAC | 1 cut(s) | 493 |
| HindIII | AAGCTT | 3 cut(s) | 553, 844, 2081 |
| HpaII | CCGG | 2 cut(s) | 578, 681 |
| HphI | GGTGA | 3 cut(s) | 1068, 1513, 1826 |
| Hpy166II | GTNNAC | 6 cut(s) | 309, 493, 1076, 1138, 1504, 1951 |
| Hpy8I | GTNNAC | 6 cut(s) | 309, 493, 1076, 1138, 1504, 1951 |
| HpyAV | CCTTC | 8 cut(s) | 183, 867, 1163, 1240, 1790, 1829, 1925, 2580 |
| HpyCH4IV | ACGT | 7 cut(s) | 328, 866, 988, 1145, 2206, 2609, 2647 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 767, 776 |
| HpyF3I | CTNAG | 9 cut(s) | 369, 429, 757, 815, 1002, 1061, 1738, 2267, 2748 |
| HpySE526I | ACGT | 7 cut(s) | 328, 866, 988, 1145, 2206, 2609, 2647 |
| HspAI | GCGC | 2 cut(s) | 1411, 1605 |
| Kzo9I | GATC | 8 cut(s) | 52, 1210, 1234, 1621, 1660, 1852, 2284, 2371 |
| LguI | GCTCTTC | 1 cut(s) | 2806 |
| LmnI | GCTCC | 4 cut(s) | 1298, 1858, 1866, 2064 |
| Lsp1109I | GCAGC | 3 cut(s) | 1415, 1589, 2081 |
| LweI | GCATC | 9 cut(s) | 342, 398, 647, 693, 811, 1639, 2074, 2081, 2717 |
| MabI | ACCWGGT | 2 cut(s) | 442, 2761 |
| MaeI | CTAG | 4 cut(s) | 33, 1400, 1890, 2352 |
| MaeII | ACGT | 7 cut(s) | 328, 866, 988, 1145, 2206, 2609, 2647 |
| MaeIII | GTNAC | 4 cut(s) | 95, 324, 596, 2643 |
| MalI | GATC | 8 cut(s) | 54, 1212, 1236, 1623, 1662, 1854, 2286, 2373 |
| MbiI | CCGCTC | 1 cut(s) | 1867 |
| MboI | GATC | 8 cut(s) | 52, 1210, 1234, 1621, 1660, 1852, 2284, 2371 |
| MboII | GAAGA | 8 cut(s) | 562, 669, 895, 956, 1723, 2002, 2793, 2814 |
| MflI | RGATCY | 3 cut(s) | 1660, 1852, 2284 |
| MhlI | GDGCHC | 1 cut(s) | 1863 |
| MlsI | TGGCCA | 1 cut(s) | 2013 |
| MluI | ACGCGT | 1 cut(s) | 290 |
| MluNI | TGGCCA | 1 cut(s) | 2013 |
| MlyI | GAGTC | 2 cut(s) | 1013, 2147 |
| MmeI | TCCRAC | 6 cut(s) | 25, 64, 153, 1223, 1750, 2383 |
| Mox20I | TGGCCA | 1 cut(s) | 2013 |
| Mph1103I | ATGCAT | 2 cut(s) | 247, 2566 |
| MroXI | GAANNNNTTC | 1 cut(s) | 2027 |
| MscI | TGGCCA | 1 cut(s) | 2013 |
| MseI | TTAA | 7 cut(s) | 669, 1085, 1424, 1802, 2100, 2360, 2863 |
| MslI | CAYNNNNRTG | 1 cut(s) | 689 |
| Msp20I | TGGCCA | 1 cut(s) | 2013 |
| MspI | CCGG | 2 cut(s) | 578, 681 |
| MspR9I | CCNGG | 8 cut(s) | 444, 579, 876, 957, 1433, 2150, 2263, 2763 |
| Mva1269I | GAATGC | 1 cut(s) | 1532 |
| MvaI | CCWGG | 7 cut(s) | 444, 876, 957, 1433, 2150, 2263, 2763 |
| MvnI | CGCG | 2 cut(s) | 292, 1209 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 767, 776 |
| NciI | CCSGG | 1 cut(s) | 579 |
| NcoI | CCATGG | 2 cut(s) | 384, 1049 |
| NdeI | CATATG | 1 cut(s) | 1009 |
| NdeII | GATC | 8 cut(s) | 52, 1210, 1234, 1621, 1660, 1852, 2284, 2371 |
| NlaIV | GGNNCC | 6 cut(s) | 105, 829, 1300, 1842, 1945, 2424 |
| NruI | TCGCGA | 1 cut(s) | 1209 |
| NsbI | TGCGCA | 1 cut(s) | 1412 |
| NsiI | ATGCAT | 2 cut(s) | 247, 2566 |
| NspI | RCATGY | 4 cut(s) | 245, 770, 2137, 2564 |
| NspV | TTCGAA | 1 cut(s) | 1672 |
| PceI | AGGCCT | 4 cut(s) | 271, 1090, 1250, 2590 |
| PciSI | GCTCTTC | 1 cut(s) | 2806 |
| PctI | GAATGC | 1 cut(s) | 1532 |
| PdmI | GAANNNNTTC | 1 cut(s) | 2027 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 1055 |
| PfoI | TCCNGGA | 1 cut(s) | 955 |
| PkrI | GCNGC | 3 cut(s) | 1430, 1604, 2096 |
| Ple19I | CGATCG | 1 cut(s) | 1213 |
| PleI | GAGTC | 2 cut(s) | 1012, 2147 |
| PpsI | GAGTC | 2 cut(s) | 1012, 2147 |
| Ppu21I | YACGTR | 2 cut(s) | 1146, 2610 |
| PshAI | GACNNNNGTC | 1 cut(s) | 951 |
| PshBI | ATTAAT | 2 cut(s) | 669, 2360 |
| PsiI | TTATAA | 1 cut(s) | 401 |
| Psp124BI | GAGCTC | 1 cut(s) | 1863 |
| Psp1406I | AACGTT | 1 cut(s) | 2206 |
| Psp6I | CCWGG | 7 cut(s) | 442, 874, 955, 1431, 2148, 2261, 2761 |
| PspFI | CCCAGC | 2 cut(s) | 1652, 2725 |
| PspGI | CCWGG | 7 cut(s) | 442, 874, 955, 1431, 2148, 2261, 2761 |
| PspN4I | GGNNCC | 6 cut(s) | 105, 829, 1300, 1842, 1945, 2424 |
| PspPI | GGNCC | 3 cut(s) | 1046, 1943, 2857 |
| PstNI | CAGNNNCTG | 2 cut(s) | 1652, 1663 |
| PsuI | RGATCY | 3 cut(s) | 1660, 1852, 2284 |
| PvuI | CGATCG | 1 cut(s) | 1213 |
| RgaI | GCGATCGC | 1 cut(s) | 1213 |
| RruI | TCGCGA | 1 cut(s) | 1209 |
| RsaI | GTAC | 8 cut(s) | 614, 813, 1139, 1148, 1204, 1263, 1597, 2629 |
| RsaNI | GTAC | 8 cut(s) | 613, 812, 1138, 1147, 1203, 1262, 1596, 2628 |
| RseI | CAYNNNNRTG | 1 cut(s) | 689 |
| SacI | GAGCTC | 1 cut(s) | 1863 |
| SapI | GCTCTTC | 1 cut(s) | 2806 |
| SaqAI | TTAA | 7 cut(s) | 669, 1085, 1424, 1802, 2100, 2360, 2863 |
| SatI | GCNGC | 3 cut(s) | 1429, 1603, 2095 |
| Sau3AI | GATC | 8 cut(s) | 52, 1210, 1234, 1621, 1660, 1852, 2284, 2371 |
| Sau96I | GGNCC | 3 cut(s) | 1046, 1943, 2857 |
| ScaI | AGTACT | 2 cut(s) | 813, 1204 |
| SchI | GAGTC | 2 cut(s) | 1013, 2147 |
| ScrFI | CCNGG | 8 cut(s) | 444, 579, 876, 957, 1433, 2150, 2263, 2763 |
| SduI | GDGCHC | 1 cut(s) | 1863 |
| SexAI | ACCWGGT | 2 cut(s) | 442, 2761 |
| SfaAI | GCGATCGC | 1 cut(s) | 1213 |
| SfaNI | GCATC | 9 cut(s) | 342, 398, 647, 693, 811, 1639, 2074, 2081, 2717 |
| SfcI | CTRYAG | 1 cut(s) | 1755 |
| SfuI | TTCGAA | 1 cut(s) | 1672 |
| SgfI | GCGATCGC | 1 cut(s) | 1213 |
| SinI | GGWCC | 1 cut(s) | 1943 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 689 |
| SmlI | CTYRAG | 4 cut(s) | 898, 1174, 1368, 1520 |
| SmoI | CTYRAG | 4 cut(s) | 898, 1174, 1368, 1520 |
| SseBI | AGGCCT | 4 cut(s) | 271, 1090, 1250, 2590 |
| SsiI | CCGC | 2 cut(s) | 1865, 2223 |
| SspMI | CTAG | 4 cut(s) | 33, 1400, 1890, 2352 |
| SstI | GAGCTC | 1 cut(s) | 1863 |
| StuI | AGGCCT | 4 cut(s) | 271, 1090, 1250, 2590 |
| StyD4I | CCNGG | 8 cut(s) | 442, 577, 874, 955, 1431, 2148, 2261, 2761 |
| StyI | CCWWGG | 5 cut(s) | 384, 423, 534, 1049, 2014 |
| TaiI | ACGT | 7 cut(s) | 331, 869, 991, 1148, 2209, 2612, 2650 |
| TaqI | TCGA | 4 cut(s) | 1672, 2068, 2281, 2840 |
| TatI | WGTACW | 4 cut(s) | 811, 1137, 1202, 2627 |
| Tru1I | TTAA | 7 cut(s) | 669, 1085, 1424, 1802, 2100, 2360, 2863 |
| Tru9I | TTAA | 7 cut(s) | 669, 1085, 1424, 1802, 2100, 2360, 2863 |
| TscAI | CASTG | 3 cut(s) | 1030, 1075, 1890 |
| TseI | GCWGC | 3 cut(s) | 1428, 1602, 2094 |
| TspGWI | ACGGA | 3 cut(s) | 476, 509, 931 |
| TspRI | CASTG | 3 cut(s) | 1030, 1075, 1890 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 1055 |
| VpaK11BI | GGWCC | 1 cut(s) | 1943 |
| VspI | ATTAAT | 2 cut(s) | 669, 2360 |
| XapI | RAATTY | 8 cut(s) | 12, 606, 805, 937, 1381, 1460, 1688, 2331 |
| XceI | RCATGY | 4 cut(s) | 245, 770, 2137, 2564 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 2146 |
| XmiI | GTMKAC | 1 cut(s) | 308 |
| XmnI | GAANNNNTTC | 1 cut(s) | 2027 |
| XspI | CTAG | 4 cut(s) | 33, 1400, 1890, 2352 |
| ZrmI | AGTACT | 2 cut(s) | 813, 1204 |
| Zsp2I | ATGCAT | 2 cut(s) | 247, 2566 |