Serine threonine-protein kinase
| Domain Name | Pfam ID | Position | E-value | Description |
|---|---|---|---|---|
| B_lectin | PF01453 | 2 - 84 | 4.1e-23 | D-mannose binding lectin |
| S_locus_glycop | PF00954 | 118 - 228 | 1.2e-29 | S-locus glycoprotein domain |
| PAN_2 | PF08276 | 249 - 317 | 6.4e-23 | PAN-like domain |
| Pkinase | PF00069 | 405 - 607 | 1.2e-42 | Protein kinase domain |
| PK_Tyr_Ser-Thr | PF07714 | 408 - 674 | 4.9e-46 | Protein tyrosine and serine/threonine kinase |
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 3 cut(s) | 1349, 1805, 2024 |
| AasI | GACNNNNNNGTC | 2 cut(s) | 709, 770 |
| Acc16I | TGCGCA | 1 cut(s) | 728 |
| AccB1I | GGYRCC | 1 cut(s) | 1726 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 371 |
| AccBSI | CCGCTC | 1 cut(s) | 1171 |
| AccI | GTMKAC | 2 cut(s) | 661, 1095 |
| AccII | CGCG | 1 cut(s) | 525 |
| AciI | CCGC | 1 cut(s) | 1169 |
| AclI | AACGTT | 1 cut(s) | 1510 |
| AclWI | GGATC | 2 cut(s) | 984, 2004 |
| AcoI | YGGCCR | 3 cut(s) | 970, 1315, 2000 |
| AcsI | RAATTY | 6 cut(s) | 115, 253, 278, 697, 1283, 1635 |
| AcuI | CTGAAG | 2 cut(s) | 984, 1349 |
| AfaI | GTAC | 4 cut(s) | 88, 96, 455, 579 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 272, 371, 968, 1572, 2108 |
| AgsI | TTSAA | 9 cut(s) | 296, 473, 492, 790, 862, 1117, 1177, 1305, 1961 |
| AjnI | CCWGG | 8 cut(s) | 271, 747, 972, 1158, 1452, 1565, 1837, 2065 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 1227, 1259, 1996, 2028 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 854, 886 |
| Alw21I | GWGCWC | 3 cut(s) | 394, 521, 1167 |
| Alw26I | GTCTC | 2 cut(s) | 1115, 1547 |
| Alw44I | GTGCAC | 1 cut(s) | 390 |
| AlwI | GGATC | 2 cut(s) | 984, 2004 |
| Ama87I | CYCGRG | 2 cut(s) | 317, 1536 |
| ApaLI | GTGCAC | 1 cut(s) | 390 |
| ApeKI | GCWGC | 5 cut(s) | 545, 744, 918, 1055, 1739 |
| ApoI | RAATTY | 6 cut(s) | 115, 253, 278, 697, 1283, 1635 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 131, 163 |
| AsiSI | GCGATCGC | 1 cut(s) | 529 |
| AspLEI | GCGC | 2 cut(s) | 729, 923 |
| AspS9I | GGNCC | 6 cut(s) | 362, 564, 1107, 1156, 1247, 2099 |
| AsuC2I | CCSGG | 1 cut(s) | 963 |
| AsuHPI | GGTGA | 2 cut(s) | 675, 829 |
| AsuII | TTCGAA | 1 cut(s) | 988 |
| AvaI | CYCGRG | 2 cut(s) | 317, 1536 |
| AvaII | GGWCC | 3 cut(s) | 1107, 1156, 1247 |
| AxyI | CCTNAGG | 1 cut(s) | 1992 |
| BaeGI | GKGCMC | 2 cut(s) | 394, 1147 |
| BalI | TGGCCA | 2 cut(s) | 972, 1317 |
| BanI | GGYRCC | 1 cut(s) | 1726 |
| BanII | GRGCYC | 1 cut(s) | 1167 |
| BauI | CACGAG | 1 cut(s) | 514 |
| BbsI | GAAGAC | 1 cut(s) | 267 |
| Bbv12I | GWGCWC | 3 cut(s) | 394, 521, 1167 |
| BbvI | GCAGC | 5 cut(s) | 557, 731, 905, 1042, 1726 |
| BccI | CCATC | 4 cut(s) | 196, 201, 1268, 1739 |
| BciT130I | CCWGG | 8 cut(s) | 273, 749, 974, 1160, 1454, 1567, 1839, 2067 |
| BciVI | GTATCC | 1 cut(s) | 793 |
| BclI | TGATCA | 2 cut(s) | 595, 1471 |
| BcnI | CCSGG | 1 cut(s) | 963 |
| BcoDI | GTCTC | 2 cut(s) | 1115, 1547 |
| BfaI | CTAG | 5 cut(s) | 582, 944, 1194, 1656, 1664 |
| BfmI | CTRYAG | 1 cut(s) | 1056 |
| BfuI | GTATCC | 1 cut(s) | 793 |
| BglII | AGATCT | 1 cut(s) | 1588 |
| BisI | GCNGC | 5 cut(s) | 546, 745, 919, 1056, 1740 |
| BlpI | GCTNAGC | 1 cut(s) | 67 |
| BlsI | GCNGC | 5 cut(s) | 547, 746, 920, 1057, 1741 |
| Bme1390I | CCNGG | 9 cut(s) | 273, 749, 963, 974, 1160, 1454, 1567, 1839, 2067 |
| Bme18I | GGWCC | 3 cut(s) | 1107, 1156, 1247 |
| BmeT110I | CYCGRG | 2 cut(s) | 317, 1536 |
| BmgT120I | GGNCC | 6 cut(s) | 362, 564, 1107, 1156, 1247, 2099 |
| BmiI | GGNNCC | 2 cut(s) | 303, 1728 |
| BmrFI | CCNGG | 9 cut(s) | 273, 749, 963, 974, 1160, 1454, 1567, 1839, 2067 |
| BmsI | GCATC | 1 cut(s) | 581 |
| BoxI | GACNNNNGTC | 1 cut(s) | 267 |
| BpiI | GAAGAC | 1 cut(s) | 267 |
| BpmI | CTGGAG | 4 cut(s) | 1181, 1689, 1704, 1817 |
| Bpu10I | CCTNAGC | 2 cut(s) | 740, 1571 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 67 |
| Bpu14I | TTCGAA | 1 cut(s) | 988 |
| BpuMI | CCSGG | 1 cut(s) | 963 |
| BsaI | GGTCTC | 1 cut(s) | 1547 |
| BsaJI | CCNNGG | 4 cut(s) | 272, 318, 365, 1318 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 1023, 1053 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 272, 371, 968, 1572, 2108 |
| Bse1I | ACTGG | 4 cut(s) | 647, 713, 1272, 1687 |
| Bse21I | CCTNAGG | 1 cut(s) | 1992 |
| BseBI | CCWGG | 8 cut(s) | 273, 749, 974, 1160, 1454, 1567, 1839, 2067 |
| BseDI | CCNNGG | 4 cut(s) | 272, 318, 365, 1318 |
| BseGI | GGATG | 5 cut(s) | 212, 725, 1834, 1987, 2135 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 272, 371, 968, 1572, 2108 |
| BseMII | CTCAG | 1 cut(s) | 1983 |
| BseNI | ACTGG | 4 cut(s) | 647, 713, 1272, 1687 |
| BseRI | GAGGAG | 3 cut(s) | 1427, 1535, 1686 |
| BseSI | GKGCMC | 2 cut(s) | 394, 1147 |
| BseXI | GCAGC | 5 cut(s) | 557, 731, 905, 1042, 1726 |
| Bsh1236I | CGCG | 1 cut(s) | 525 |
| Bsh1285I | CGRYCG | 1 cut(s) | 529 |
| BshNI | GGYRCC | 1 cut(s) | 1726 |
| BsiEI | CGRYCG | 1 cut(s) | 529 |
| BsiHKAI | GWGCWC | 3 cut(s) | 394, 521, 1167 |
| BsiHKCI | CYCGRG | 2 cut(s) | 317, 1536 |
| BsiSI | CCGG | 2 cut(s) | 797, 962 |
| BslFI | GGGAC | 3 cut(s) | 224, 1276, 1723 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 272, 371, 968, 1572, 2108 |
| BsmAI | GTCTC | 2 cut(s) | 1115, 1547 |
| BsmFI | GGGAC | 3 cut(s) | 224, 1276, 1723 |
| BsmI | GAATGC | 3 cut(s) | 848, 1077, 1956 |
| Bso31I | GGTCTC | 1 cut(s) | 1547 |
| BsoBI | CYCGRG | 2 cut(s) | 317, 1536 |
| Bsp119I | TTCGAA | 1 cut(s) | 988 |
| Bsp1286I | GDGCHC | 4 cut(s) | 394, 521, 1147, 1167 |
| Bsp143I | GATC | 9 cut(s) | 526, 595, 937, 976, 1353, 1471, 1588, 1675, 1996 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 67 |
| Bsp19I | CCATGG | 1 cut(s) | 365 |
| BspACI | CCGC | 1 cut(s) | 1169 |
| BspCNI | CTCAG | 1 cut(s) | 1984 |
| BspFNI | CGCG | 1 cut(s) | 525 |
| BspHI | TCATGA | 1 cut(s) | 1335 |
| BspLI | GGNNCC | 2 cut(s) | 303, 1728 |
| BspMAI | CTGCAG | 1 cut(s) | 1060 |
| BspPI | GGATC | 2 cut(s) | 984, 2004 |
| BspQI | GCTCTTC | 1 cut(s) | 1178 |
| BspT104I | TTCGAA | 1 cut(s) | 988 |
| BspT107I | GGYRCC | 1 cut(s) | 1726 |
| BspTNI | GGTCTC | 1 cut(s) | 1547 |
| BsrBI | CCGCTC | 1 cut(s) | 1171 |
| BsrI | ACTGG | 4 cut(s) | 647, 713, 1272, 1687 |
| BssECI | CCNNGG | 4 cut(s) | 272, 318, 365, 1318 |
| BssMI | GATC | 9 cut(s) | 526, 595, 937, 976, 1353, 1471, 1588, 1675, 1996 |
| BssSI | CACGAG | 1 cut(s) | 514 |
| BssT1I | CCWWGG | 2 cut(s) | 365, 1318 |
| Bst2BI | CACGAG | 1 cut(s) | 514 |
| Bst2UI | CCWGG | 8 cut(s) | 273, 749, 974, 1160, 1454, 1567, 1839, 2067 |
| Bst4CI | ACNGT | 2 cut(s) | 869, 1494 |
| Bst6I | CTCTTC | 3 cut(s) | 1178, 1289, 1404 |
| BstBI | TTCGAA | 1 cut(s) | 988 |
| BstC8I | GCNNGC | 2 cut(s) | 970, 1979 |
| BstDSI | CCRYGG | 1 cut(s) | 365 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 2106 |
| BstF5I | GGATG | 5 cut(s) | 212, 725, 1834, 1987, 2135 |
| BstFNI | CGCG | 1 cut(s) | 525 |
| BstHHI | GCGC | 2 cut(s) | 729, 923 |
| BstKTI | GATC | 9 cut(s) | 529, 598, 940, 979, 1356, 1474, 1591, 1678, 1999 |
| BstMAI | GTCTC | 2 cut(s) | 1115, 1547 |
| BstMBI | GATC | 9 cut(s) | 526, 595, 937, 976, 1353, 1471, 1588, 1675, 1996 |
| BstMCI | CGRYCG | 1 cut(s) | 529 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 77, 1314 |
| BstNI | CCWGG | 8 cut(s) | 273, 749, 974, 1160, 1454, 1567, 1839, 2067 |
| BstNSI | RCATGY | 2 cut(s) | 1423, 1441 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 267 |
| BstSCI | CCNGG | 9 cut(s) | 271, 747, 961, 972, 1158, 1452, 1565, 1837, 2065 |
| BstSFI | CTRYAG | 1 cut(s) | 1056 |
| BstSLI | GKGCMC | 2 cut(s) | 394, 1147 |
| BstUI | CGCG | 1 cut(s) | 525 |
| BstV1I | GCAGC | 5 cut(s) | 557, 731, 905, 1042, 1726 |
| BstV2I | GAAGAC | 1 cut(s) | 267 |
| BstX2I | RGATCY | 2 cut(s) | 976, 1588 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 693 |
| BstYI | RGATCY | 2 cut(s) | 976, 1588 |
| Bsu36I | CCTNAGG | 1 cut(s) | 1992 |
| BsuI | GTATCC | 1 cut(s) | 793 |
| BtgI | CCRYGG | 1 cut(s) | 365 |
| BtsCI | GGATG | 5 cut(s) | 212, 725, 1834, 1987, 2135 |
| BtsI | GCAGTG | 1 cut(s) | 1065 |
| BtsIMutI | CAGTG | 3 cut(s) | 391, 1065, 1265 |
| Cac8I | GCNNGC | 2 cut(s) | 970, 1979 |
| CciI | TCATGA | 1 cut(s) | 1335 |
| CfoI | GCGC | 2 cut(s) | 729, 923 |
| Cfr13I | GGNCC | 6 cut(s) | 362, 564, 1107, 1156, 1247, 2099 |
| CsiI | ACCWGGT | 1 cut(s) | 2065 |
| Csp6I | GTAC | 4 cut(s) | 87, 95, 454, 578 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 1505, 1540 |
| CviQI | GTAC | 4 cut(s) | 87, 95, 454, 578 |
| DpnI | GATC | 9 cut(s) | 528, 597, 939, 978, 1355, 1473, 1590, 1677, 1998 |
| DpnII | GATC | 9 cut(s) | 526, 595, 937, 976, 1353, 1471, 1588, 1675, 1996 |
| DrdI | GACNNNNNNGTC | 2 cut(s) | 709, 770 |
| DseDI | GACNNNNNNGTC | 2 cut(s) | 709, 770 |
| EaeI | YGGCCR | 3 cut(s) | 970, 1315, 2000 |
| Eam1104I | CTCTTC | 3 cut(s) | 1178, 1289, 1404 |
| EarI | CTCTTC | 3 cut(s) | 1178, 1289, 1404 |
| Ecl136II | GAGCTC | 1 cut(s) | 1165 |
| Eco130I | CCWWGG | 2 cut(s) | 365, 1318 |
| Eco147I | AGGCCT | 2 cut(s) | 406, 1894 |
| Eco24I | GRGCYC | 1 cut(s) | 1167 |
| Eco31I | GGTCTC | 1 cut(s) | 1547 |
| Eco32I | GATATC | 3 cut(s) | 20, 898, 949 |
| Eco47I | GGWCC | 3 cut(s) | 1107, 1156, 1247 |
| Eco53kI | GAGCTC | 1 cut(s) | 1165 |
| Eco57I | CTGAAG | 2 cut(s) | 984, 1349 |
| Eco81I | CCTNAGG | 1 cut(s) | 1992 |
| Eco88I | CYCGRG | 2 cut(s) | 317, 1536 |
| EcoICRI | GAGCTC | 1 cut(s) | 1165 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 2106 |
| EcoO109I | RGGNCCY | 3 cut(s) | 564, 1156, 2099 |
| EcoRI | GAATTC | 1 cut(s) | 278 |
| EcoRII | CCWGG | 8 cut(s) | 271, 747, 972, 1158, 1452, 1565, 1837, 2065 |
| EcoRV | GATATC | 3 cut(s) | 20, 898, 949 |
| EcoT14I | CCWWGG | 2 cut(s) | 365, 1318 |
| EcoT22I | ATGCAT | 2 cut(s) | 313, 574 |
| EcoT38I | GRGCYC | 1 cut(s) | 1167 |
| ErhI | CCWWGG | 2 cut(s) | 365, 1318 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 1953, 1985, 1996, 2028 |
| FaqI | GGGAC | 3 cut(s) | 224, 1276, 1723 |
| FauI | CCCGC | 1 cut(s) | 1176 |
| FauNDI | CATATG | 1 cut(s) | 1714 |
| FbaI | TGATCA | 2 cut(s) | 595, 1471 |
| FblI | GTMKAC | 2 cut(s) | 661, 1095 |
| Fnu4HI | GCNGC | 5 cut(s) | 546, 745, 919, 1056, 1740 |
| FokI | GGATG | 5 cut(s) | 219, 732, 1821, 1974, 2122 |
| FriOI | GRGCYC | 1 cut(s) | 1167 |
| Fsp4HI | GCNGC | 5 cut(s) | 546, 745, 919, 1056, 1740 |
| FspBI | CTAG | 5 cut(s) | 582, 944, 1194, 1656, 1664 |
| FspI | TGCGCA | 1 cut(s) | 728 |
| GlaI | GCGC | 2 cut(s) | 728, 922 |
| GluI | GCNGC | 5 cut(s) | 546, 745, 919, 1056, 1740 |
| GsuI | CTGGAG | 4 cut(s) | 1181, 1689, 1704, 1817 |
| HapII | CCGG | 2 cut(s) | 797, 962 |
| HhaI | GCGC | 2 cut(s) | 729, 923 |
| Hin6I | GCGC | 2 cut(s) | 727, 921 |
| HinP1I | GCGC | 2 cut(s) | 727, 921 |
| HindIII | AAGCTT | 3 cut(s) | 64, 160, 1226 |
| HinfI | GANTC | 9 cut(s) | 40, 107, 381, 669, 1387, 1562, 1627, 1910, 2117 |
| HpaII | CCGG | 2 cut(s) | 797, 962 |
| HphI | GGTGA | 2 cut(s) | 675, 829 |
| Hpy166II | GTNNAC | 9 cut(s) | 60, 392, 454, 662, 780, 820, 1024, 1096, 1255 |
| Hpy188III | TCNNGA | 6 cut(s) | 111, 317, 1068, 1336, 1708, 1991 |
| Hpy8I | GTNNAC | 9 cut(s) | 60, 392, 454, 662, 780, 820, 1024, 1096, 1255 |
| HpyAV | CCTTC | 6 cut(s) | 183, 1229, 1806, 1880, 1884, 2087 |
| HpyCH4III | ACNGT | 2 cut(s) | 869, 1494 |
| HpyCH4IV | ACGT | 2 cut(s) | 1510, 1951 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 77, 1314 |
| HpySE526I | ACGT | 2 cut(s) | 1510, 1951 |
| HspAI | GCGC | 2 cut(s) | 727, 921 |
| Ksp22I | TGATCA | 2 cut(s) | 595, 1471 |
| Kzo9I | GATC | 9 cut(s) | 526, 595, 937, 976, 1353, 1471, 1588, 1675, 1996 |
| LguI | GCTCTTC | 1 cut(s) | 1178 |
| LmnI | GCTCC | 3 cut(s) | 614, 1162, 1170 |
| Lsp1109I | GCAGC | 5 cut(s) | 557, 731, 905, 1042, 1726 |
| LweI | GCATC | 1 cut(s) | 581 |
| MabI | ACCWGGT | 1 cut(s) | 2065 |
| MaeI | CTAG | 5 cut(s) | 582, 944, 1194, 1656, 1664 |
| MaeII | ACGT | 2 cut(s) | 1510, 1951 |
| MaeIII | GTNAC | 6 cut(s) | 133, 186, 638, 681, 711, 1947 |
| MalI | GATC | 9 cut(s) | 528, 597, 939, 978, 1355, 1473, 1590, 1677, 1998 |
| MbiI | CCGCTC | 1 cut(s) | 1171 |
| MboI | GATC | 9 cut(s) | 526, 595, 937, 976, 1353, 1471, 1588, 1675, 1996 |
| MboII | GAAGA | 8 cut(s) | 211, 272, 1148, 1165, 1306, 1421, 1453, 1496 |
| MflI | RGATCY | 2 cut(s) | 976, 1588 |
| MhlI | GDGCHC | 4 cut(s) | 394, 521, 1147, 1167 |
| MlsI | TGGCCA | 2 cut(s) | 972, 1317 |
| MluNI | TGGCCA | 2 cut(s) | 972, 1317 |
| Mox20I | TGGCCA | 2 cut(s) | 972, 1317 |
| Mph1103I | ATGCAT | 2 cut(s) | 313, 574 |
| MscI | TGGCCA | 2 cut(s) | 972, 1317 |
| MseI | TTAA | 3 cut(s) | 228, 503, 1326 |
| MslI | CAYNNNNRTG | 4 cut(s) | 718, 989, 1424, 1790 |
| Msp20I | TGGCCA | 2 cut(s) | 972, 1317 |
| MspI | CCGG | 2 cut(s) | 797, 962 |
| MspR9I | CCNGG | 9 cut(s) | 273, 749, 963, 974, 1160, 1454, 1567, 1839, 2067 |
| Mva1269I | GAATGC | 3 cut(s) | 848, 1077, 1956 |
| MvaI | CCWGG | 8 cut(s) | 273, 749, 974, 1160, 1454, 1567, 1839, 2067 |
| MvnI | CGCG | 1 cut(s) | 525 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 77, 1314 |
| NciI | CCSGG | 1 cut(s) | 963 |
| NcoI | CCATGG | 1 cut(s) | 365 |
| NdeI | CATATG | 1 cut(s) | 1714 |
| NdeII | GATC | 9 cut(s) | 526, 595, 937, 976, 1353, 1471, 1588, 1675, 1996 |
| NlaIV | GGNNCC | 2 cut(s) | 303, 1728 |
| NmuCI | GTSAC | 3 cut(s) | 133, 681, 711 |
| NsbI | TGCGCA | 1 cut(s) | 728 |
| NsiI | ATGCAT | 2 cut(s) | 313, 574 |
| NspI | RCATGY | 2 cut(s) | 1423, 1441 |
| NspV | TTCGAA | 1 cut(s) | 988 |
| PagI | TCATGA | 1 cut(s) | 1335 |
| PceI | AGGCCT | 2 cut(s) | 406, 1894 |
| PciSI | GCTCTTC | 1 cut(s) | 1178 |
| PctI | GAATGC | 3 cut(s) | 848, 1077, 1956 |
| PfeI | GAWTC | 9 cut(s) | 40, 107, 381, 669, 1387, 1562, 1627, 1910, 2117 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 371 |
| PkrI | GCNGC | 5 cut(s) | 547, 746, 920, 1057, 1741 |
| Ple19I | CGATCG | 1 cut(s) | 529 |
| PpuMI | RGGWCCY | 1 cut(s) | 1156 |
| PshAI | GACNNNNGTC | 1 cut(s) | 267 |
| PsiI | TTATAA | 3 cut(s) | 1349, 1805, 2024 |
| Psp124BI | GAGCTC | 1 cut(s) | 1167 |
| Psp1406I | AACGTT | 1 cut(s) | 1510 |
| Psp5II | RGGWCCY | 1 cut(s) | 1156 |
| Psp6I | CCWGG | 8 cut(s) | 271, 747, 972, 1158, 1452, 1565, 1837, 2065 |
| PspGI | CCWGG | 8 cut(s) | 271, 747, 972, 1158, 1452, 1565, 1837, 2065 |
| PspN4I | GGNNCC | 2 cut(s) | 303, 1728 |
| PspPI | GGNCC | 6 cut(s) | 362, 564, 1107, 1156, 1247, 2099 |
| PspPPI | RGGWCCY | 1 cut(s) | 1156 |
| PstI | CTGCAG | 1 cut(s) | 1060 |
| PsuI | RGATCY | 2 cut(s) | 976, 1588 |
| PvuI | CGATCG | 1 cut(s) | 529 |
| RgaI | GCGATCGC | 1 cut(s) | 529 |
| RsaI | GTAC | 4 cut(s) | 88, 96, 455, 579 |
| RsaNI | GTAC | 4 cut(s) | 87, 95, 454, 578 |
| RseI | CAYNNNNRTG | 4 cut(s) | 718, 989, 1424, 1790 |
| SacI | GAGCTC | 1 cut(s) | 1167 |
| SapI | GCTCTTC | 1 cut(s) | 1178 |
| SaqAI | TTAA | 3 cut(s) | 228, 503, 1326 |
| SatI | GCNGC | 5 cut(s) | 546, 745, 919, 1056, 1740 |
| Sau3AI | GATC | 9 cut(s) | 526, 595, 937, 976, 1353, 1471, 1588, 1675, 1996 |
| Sau96I | GGNCC | 6 cut(s) | 362, 564, 1107, 1156, 1247, 2099 |
| ScrFI | CCNGG | 9 cut(s) | 273, 749, 963, 974, 1160, 1454, 1567, 1839, 2067 |
| SduI | GDGCHC | 4 cut(s) | 394, 521, 1147, 1167 |
| SexAI | ACCWGGT | 1 cut(s) | 2065 |
| SfaAI | GCGATCGC | 1 cut(s) | 529 |
| SfaNI | GCATC | 1 cut(s) | 581 |
| SfcI | CTRYAG | 1 cut(s) | 1056 |
| SfuI | TTCGAA | 1 cut(s) | 988 |
| SgfI | GCGATCGC | 1 cut(s) | 529 |
| SinI | GGWCC | 3 cut(s) | 1107, 1156, 1247 |
| SmiMI | CAYNNNNRTG | 4 cut(s) | 718, 989, 1424, 1790 |
| SseBI | AGGCCT | 2 cut(s) | 406, 1894 |
| SsiI | CCGC | 1 cut(s) | 1169 |
| SspI | AATATT | 1 cut(s) | 350 |
| SspMI | CTAG | 5 cut(s) | 582, 944, 1194, 1656, 1664 |
| SstI | GAGCTC | 1 cut(s) | 1167 |
| StuI | AGGCCT | 2 cut(s) | 406, 1894 |
| StyD4I | CCNGG | 9 cut(s) | 271, 747, 961, 972, 1158, 1452, 1565, 1837, 2065 |
| StyI | CCWWGG | 2 cut(s) | 365, 1318 |
| TaaI | ACNGT | 2 cut(s) | 869, 1494 |
| TaiI | ACGT | 2 cut(s) | 1513, 1954 |
| TaqI | TCGA | 3 cut(s) | 165, 426, 988 |
| TatI | WGTACW | 2 cut(s) | 86, 453 |
| TfiI | GAWTC | 9 cut(s) | 40, 107, 381, 669, 1387, 1562, 1627, 1910, 2117 |
| Tru1I | TTAA | 3 cut(s) | 228, 503, 1326 |
| Tru9I | TTAA | 3 cut(s) | 228, 503, 1326 |
| TscAI | CASTG | 3 cut(s) | 391, 1065, 1272 |
| TseFI | GTSAC | 3 cut(s) | 133, 681, 711 |
| TseI | GCWGC | 5 cut(s) | 545, 744, 918, 1055, 1739 |
| Tsp45I | GTSAC | 3 cut(s) | 133, 681, 711 |
| TspRI | CASTG | 3 cut(s) | 391, 1065, 1272 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 371 |
| VneI | GTGCAC | 1 cut(s) | 390 |
| VpaK11BI | GGWCC | 3 cut(s) | 1107, 1156, 1247 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 2106 |
| XapI | RAATTY | 6 cut(s) | 115, 253, 278, 697, 1283, 1635 |
| XceI | RCATGY | 2 cut(s) | 1423, 1441 |
| XcmI | CCANNNNNNNNNTGG | 4 cut(s) | 1450, 1523, 1856, 2146 |
| XmiI | GTMKAC | 2 cut(s) | 661, 1095 |
| XspI | CTAG | 5 cut(s) | 582, 944, 1194, 1656, 1664 |
| Zsp2I | ATGCAT | 2 cut(s) | 313, 574 |