Rroxscaffold_1G00052720

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000207
Physical Location & Seq
Forward (+)
73475879 .. 73479266
3388 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00052720.1

Sequence Viewer

Length: 2469 bp
ATGCAAGTGTTTAGAAGCCTTTTTGTGTGCTCATTCCTATTCTCCTTCCTAGGGACCTCAAGTTCAAACACAATAGACACTCTGACTCCAAGTCAATGTATTAGAGATGGTGAAACTCTAGTTTCAGCAGGTGGGAGCTTTGAACTGGGATTCTTTAGCCCTGGTGAAGTAAAAGGCAGATACTTGGGGATATGGTATGTTAATTCTAATGTGACTATTGTGTGGGTAGCCAACAGAGAAACACCTCTTCATGATTCTTCAGGCATTTTAAAGCTGACTGATCAAGGAGATCTTGTCCTTCTCAATAGCTCAAACAGTTCGGTATGGTCATCCAATTCCTCAAAAACTGCAGAGAATCCTGTATCACAGCTCTTGGATTCGGGAAATCTTGTTGTGAAAGATGAAAACGAAACCAACCCTGATAACTTCTTGTGGCAGAGTTTTGATTATCCTTCTGATACACTCCTACCTGAAATGAAGCTTGGTTGGAACTTAGTTACCGGTTTAGAGAGTTATGTCTCATCTTGGAAGAGCACAGAAGATCCAGCTCCAGGAGAGTTTTCAGTACGGATGGATCTTCATGGTTTCCCACAAATTTTTCTTATGAAGGGAGCTAAGATACAGAGTAGAGGAGGGTCATGGAACGGCTTTCATGTAACAGGATATGGATATCCTAGTCCACTAGAACCAAACTCACCATCTGGGTTTCAATTTGTGTTGAATGAGGATGAGGTCTATTCTGAATACAAACTCATAAACAGATCATTGTTCTCGAGATATGTGTTGAGTCCCTTAGGTATTGCCCAGCGATTTGCATGGGTCGACCAAACACATATTTGGGAACTTTCCTCTACAGTTCTAGCAGATCAGTGTGATAGTTATGCCTTGTGTGGCGCATATACGAGGTGCAATATCAATACCTCTCCCATCTGTGCATGCTTGCAGGGATTTGTACCAAAATCTCCAAGAGATTGGAATTCAGGAAATTGGTCTAATGGGTGTGTTCGCAAGACTCCTTTAACTTGCAGTGAAGATGGCTTCTTAAATTTCACTAGGTTGAAGTTGCCAGACACATCTTCTTCTTGGTTTGATACAAGCATGAGCCTTGAGGAGTGCAAGGTTTTATGTTTGGAAAACTGCTCGTGTACGGCATATGCAAATACAGATATCAGGGATGGAGGAAGTGGCTGCTTGCTTTGGTTTGAGAACCTCATTGACATAAAAGAATTCACTCCTGGTGGTGGTCAAGACCTCTATATACGGATGGCTGCTTCAGAACTAGGTCATACTGAGGAAATGAACAAGTTGAACAAGAAAAAGCTAGCTTTTATTATTCTCAGCTGCACAGTTTTCCTCATGGGACTATTAATCATTGGATTGCTCTTATATAGACAGAGGAAGAAACTCAGCGATCAAGAAGTCTGGAGAAACTACAAGAAAGGTTACCTTGGGGAAGATGGGGAAGACATGGAGTTGCCAACATTTGACTTGACAACAATCGGTAACGCCACCAATGACTTTTCAAGCAGCAACAAGCTGGGAGAAGGTGGTTTTGGACCAGTGTTCAAGGGCACATTAATAGGAGGAAAGGAAATTGCAGTAAAGAGGTTGTCTGAGAATTCTGGACAGGGAGTAAGAGAGTTCAAAAACGAAGTCATACTAATAGCTGGACTTCAGAACCGCAATCTTGTAAAGCTTCTGGGTTGTTGCACTGAGGACAATGAGAAAATGTTAATATACGAATTCTTGCCCAATAGAAGCTTGGACTTCTTTATTTTTGACCAAGAACGAAAGAAGTTGCTCGATTGGCCCAAGTGCTTCAACATTATTGATGGAATTGCTCGAGGGCTCCTATATCTTCACCAAGACTCAAGATTAAGGATTATCCATAGAGATATGAAAGCTAGCAATATTTTGCTCGATAGTAATATGAACCCAAAGATTTCAGACTTCGGCCTGGCAAAAACATTTGGCGGTGATGAAAGCCGATCCAAAACAAACATAGTGGCTGGAACATATGGTTATATGGCCCCTGAATATGCAGTAGATGGAATTTTCTCTATGAAATCTGATGTATTTAGCTTTGGAGTTGTACTATTAGAGATATTGAGTAGGGAAAGGAACAGGGGATTCTGTCATGCAGATCACCATCATAACCTTCTTGGACATGCATGGACACTGTGGATGCAAGATAAACAACTGGAATTAATTGATAAGACGTTACGTGATTCATGCACCATATCGGAAGTATTAAGGTGTCTTCATGTGGGACTTTTATGTGTGCAACGAAGACCTGAAGAGAGACCAAACATGTCAAAAGTTGTTGTCATGTTGAGCAATGAGGTAACCTTGCCTCCACCAAAGCAGCCTGGTTTTTACTCAGAACGGAGTCTACCTGAATCACCATCGATGATGCATTACCCGTCTTCAGAAAATAATTTCACCATTACATTGTTGGAGTCTAGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

822

Amino Acids

92.24

Weight (kDa)

5.46

Isoelectric Point (pI)

49.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 72 - 177 1.3e-39 D-mannose binding lectin
S_locus_glycop PF00954 210 - 321 1.2e-21 S-locus glycoprotein domain
PAN_2 PF08276 342 - 407 4.9e-22 PAN-like domain
Pkinase PF00069 508 - 774 5.3e-40 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 510 - 776 2.8e-45 Protein tyrosine and serine/threonine kinase
DUF3403 PF11883 779 - 822 3.5e-07 Domain of unknown function (DUF3403)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000207)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G27300
fragaria_vesca FvH4_3g15930 FvH4_3g15980 FvH4_3g15980 FvH4_3g15980 FvH4_3g15980 FvH4_3g21350 FvH4_3g21400 FvH4_3g21400 FvH4_6g12332
malus_domestica MD02G1167100.v1.1 MD02G1167200.v1.1 MD03G1185600.v1.1 MD05G1213900.v1.1 MD05G1214100.v1.1 MD05G1214200.v1.1 MD05G1214700.v1.1 MD05G1216300.v1.1 MD05G1216800.v1.1 MD05G1217300.v1.1 MD05G1218000.v1.1 MD11G1231100.v1.1 MD11G1231200.v1.1 MD11G1231400.v1.1 MD11G1231500.v1.1 MD11G1232400.v1.1 MD11G1232500.v1.1
prunus_persica Prupe.4G142300_v2.0.a1 Prupe.4G142400_v2.0.a1 Prupe.4G142500_v2.0.a1 Prupe.4G142600_v2.0.a1 Prupe.4G142800_v2.0.a1 Prupe.4G195000_v2.0.a1 Prupe.4G195000_v2.0.a1 Prupe.4G195000_v2.0.a1 Prupe.4G195100_v2.0.a1 Prupe.4G195300_v2.0.a1 Prupe.4G195300_v2.0.a1 Prupe.4G195300_v2.0.a1 Prupe.4G195400_v2.0.a1 Prupe.4G195400_v2.0.a1 Prupe.4G195500_v2.0.a1 Prupe.4G195700_v2.0.a1 Prupe.4G195900_v2.0.a1
pyrus_communis pycom03g16540 pycom05g19920 pycom11g20460 pycom11g20480 pycom11g20490 pycom11g20500 pycom11g20530
rosa_chinensis RchiOBHm_Chr2g0139311 RchiOBHm_Chr2g0139351 RchiOBHm_Chr5g0026801 RchiOBHm_Chr5g0026851 RchiOBHm_Chr5g0026891 RchiOBHm_Chr5g0026911 RchiOBHm_Chr5g0036571 RchiOBHm_Chr5g0036591 RchiOBHm_Chr5g0036721 RchiOBHm_Chr5g0036741 RchiOBHm_Chr7g0222981
rosa_laevigata RLG00000002006 RLG00000019807 RLG00000032964 RLG00000032968 RLG00000032969 RLG00000033706 RLG00000033708 RLG00000033712 RLG00000033716
rosa_multiflora Rmu_co8256651.1_g000001 Rmu_co8279377.1_g000001 Rmu_co8429919.1_g000001 Rmu_co8490103.1_g000001 Rmu_sc0000774.1_g000002 Rmu_sc0002525.1_g000012 Rmu_sc0004223.1_g000008 Rmu_sc0004390.1_g000002 Rmu_sc0006059.1_g000014 Rmu_sc0006059.1_g000024 Rmu_sc0006514.1_g000020 Rmu_sc0014333.1_g000002 Rmu_ssc0000172.1_g000018 Rmu_ssc0000172.1_g000029
rosa_roxburghii Rroxscaffold_1G00044230 Rroxscaffold_1G00044260 Rroxscaffold_1G00044320 Rroxscaffold_1G00044340 Rroxscaffold_1G00052560 Rroxscaffold_1G00052570 Rroxscaffold_1G00052660 Rroxscaffold_1G00052720 Rroxscaffold_2G00105450 Rroxscaffold_2G00105460
rosa_rugosa Rorug02G0356900 Rorug02G0357000 Rorug02G0357100 Rorug02G0357200 Rorug02G0357300 Rorug02G0357400 Rorug02G0357500 Rorug02G0357600 Rorug05G0095400 Rorug05G0095500 Rorug05G0095500 Rorug05G0095600 Rorug05G0095700 Rorug05G0095800 Rorug05G0157700 Rorug05G0157800
rosa_samantha Rh2AG406800 Rh2AG406900 Rh2BG417300 Rh2CG392900 Rh2DG426200 Rh5AG189300 Rh5AG249400 Rh5AG250100 Rh5BG186300 Rh5BG186900 Rh5BG187000 Rh5BG249500 Rh5BG250600 Rh5BG250900 Rh5BG251500 Rh5BG251800 Rh5CG205500 Rh5CG206300 Rh5CG206700 Rh5CG283000 Rh5CG283300 Rh5CG283500 Rh5DG188500 Rh5DG188700 Rh5DG258700 Rh5DG258900 Rh5DG259600 Rh5DG259900 Rh7CG375800 Rh7DG352500
rosa_wichuraiana Rw2G032960 Rw2G032970 Rw2G033220 Rw2G033230 Rw5G017130 Rw5G017230 Rw5G022940 Rw5G022960 Rw5G022980 Rw7G030420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 119
Acc36I ACCTGC 1 cut(s) 119
AccI GTMKAC 2 cut(s) 822, 2392
AciI CCGC 2 cut(s) 1681, 1974
AclWI GGATC 3 cut(s) 536, 582, 1983
AcsI RAATTY 7 cut(s) 594, 976, 1045, 1226, 1618, 1742, 2052
AcuI CTGAAG 5 cut(s) 243, 1257, 1658, 2316, 2412
AfaI GTAC 4 cut(s) 567, 954, 1147, 2094
AfiI CCNNNNNNNGG 3 cut(s) 51, 551, 1241
AflIII ACRYGT 1 cut(s) 2310
AgeI ACCGGT 1 cut(s) 500
AhdI GACNNNNNGTC 1 cut(s) 90
AjnI CCWGG 5 cut(s) 160, 550, 1234, 1956, 2368
AjuI GAANNNNNNNTTGG 4 cut(s) 465, 497, 1536, 1568
AloI GAACNNNNNNTCC 2 cut(s) 46, 78
Alw21I GWGCWC 2 cut(s) 32, 536
Alw26I GTCTC 2 cut(s) 523, 2296
AlwI GGATC 3 cut(s) 536, 582, 1983
Ama87I CYCGRG 2 cut(s) 772, 1842
AoxI GGCC 3 cut(s) 1808, 1954, 2028
ApeKI GCWGC 5 cut(s) 1188, 1268, 1341, 1527, 2365
ApoI RAATTY 7 cut(s) 594, 976, 1045, 1226, 1618, 1742, 2052
ArsI GACNNNNNNTTYG 2 cut(s) 1638, 1670
AseI ATTAAT 3 cut(s) 1367, 1577, 2207
AsiGI ACCGGT 1 cut(s) 500
AspA2I CCTAGG 1 cut(s) 49
AspLEI GCGC 1 cut(s) 896
AspS9I GGNCC 4 cut(s) 54, 1556, 1809, 2029
AsuHPI GGTGA 8 cut(s) 122, 176, 687, 1853, 1988, 2138, 2394, 2434
AsuNHI GCTAGC 2 cut(s) 1321, 1904
AvaI CYCGRG 2 cut(s) 772, 1842
AvaII GGWCC 2 cut(s) 54, 1556
AvrII CCTAGG 1 cut(s) 49
AxyI CCTNAGG 1 cut(s) 793
BaeGI GKGCMC 1 cut(s) 1574
BanII GRGCYC 1 cut(s) 1851
BauI CACGAG 1 cut(s) 1141
BbsI GAAGAC 4 cut(s) 1470, 2252, 2296, 2418
Bbv12I GWGCWC 2 cut(s) 32, 536
BbvI GCAGC 5 cut(s) 1175, 1255, 1328, 1539, 2377
BceAI ACGGC 2 cut(s) 661, 1164
BciT130I CCWGG 5 cut(s) 162, 552, 1236, 1958, 2370
BclI TGATCA 1 cut(s) 280
BcoDI GTCTC 2 cut(s) 523, 2296
BfmI CTRYAG 2 cut(s) 348, 852
BfuAI ACCTGC 1 cut(s) 119
BglII AGATCT 1 cut(s) 289
BisI GCNGC 5 cut(s) 1189, 1269, 1342, 1528, 2366
BlnI CCTAGG 1 cut(s) 49
BlsI GCNGC 5 cut(s) 1190, 1270, 1343, 1529, 2367
Bme1390I CCNGG 5 cut(s) 162, 552, 1236, 1958, 2370
Bme18I GGWCC 2 cut(s) 54, 1556
BmeRI GACNNNNNGTC 1 cut(s) 90
BmeT110I CYCGRG 2 cut(s) 772, 1842
BmgT120I GGNCC 4 cut(s) 54, 1556, 1809, 2029
BmiI GGNNCC 3 cut(s) 55, 1850, 2031
BmrFI CCNGG 5 cut(s) 162, 552, 1236, 1958, 2370
BmrI ACTGGG 1 cut(s) 155
BmsI GCATC 2 cut(s) 2175, 2403
BmtI GCTAGC 2 cut(s) 1325, 1908
BmuI ACTGGG 1 cut(s) 155
BpiI GAAGAC 4 cut(s) 1470, 2252, 2296, 2418
BpmI CTGGAG 2 cut(s) 534, 1444
BpuEI CTTGAG 3 cut(s) 43, 1127, 1855
Bsa29I ATCGAT 1 cut(s) 2408
BsaAI YACGTR 1 cut(s) 2225
BsaBI GATNNNNATC 1 cut(s) 2148
BsaI GGTCTC 1 cut(s) 2296
BsaJI CCNNGG 3 cut(s) 49, 160, 1447
BsaWI WCCGGW 1 cut(s) 500
BsaXI ACNNNNNCTCC 7 cut(s) 70, 100, 279, 309, 2338, 2368, 2450
Bsc4I CCNNNNNNNGG 3 cut(s) 51, 551, 1241
Bse118I RCCGGY 1 cut(s) 500
Bse1I ACTGG 3 cut(s) 150, 1559, 2205
Bse21I CCTNAGG 1 cut(s) 793
Bse3DI GCAATG 1 cut(s) 2344
Bse8I GATNNNNATC 1 cut(s) 2148
BseBI CCWGG 5 cut(s) 162, 552, 1236, 1958, 2370
BseCI ATCGAT 1 cut(s) 2408
BseDI CCNNGG 3 cut(s) 49, 160, 1447
BseGI GGATG 6 cut(s) 329, 576, 733, 1180, 1269, 2190
BseJI GATNNNNATC 1 cut(s) 2148
BseLI CCNNNNNNNGG 3 cut(s) 51, 551, 1241
BseMI GCAATG 1 cut(s) 2344
BseMII CTCAG 6 cut(s) 1281, 1351, 1420, 1605, 1704, 2394
BseNI ACTGG 3 cut(s) 150, 1559, 2205
BseRI GAGGAG 2 cut(s) 645, 1124
BseSI GKGCMC 1 cut(s) 1574
BseXI GCAGC 5 cut(s) 1175, 1255, 1328, 1539, 2377
BseYI CCCAGC 2 cut(s) 804, 1537
BsgI GTGCAG 1 cut(s) 1327
BshFI GGCC 3 cut(s) 1810, 1956, 2030
BshTI ACCGGT 1 cut(s) 500
BshVI ATCGAT 1 cut(s) 2408
BsiHKAI GWGCWC 2 cut(s) 32, 536
BsiHKCI CYCGRG 2 cut(s) 772, 1842
BsiSI CCGG 1 cut(s) 501
BslFI GGGAC 4 cut(s) 67, 774, 1374, 2283
BslI CCNNNNNNNGG 3 cut(s) 51, 551, 1241
BsmAI GTCTC 2 cut(s) 523, 2296
BsmFI GGGAC 4 cut(s) 67, 774, 1374, 2283
BsnI GGCC 3 cut(s) 1810, 1956, 2030
Bso31I GGTCTC 1 cut(s) 2296
BsoBI CYCGRG 2 cut(s) 772, 1842
Bsp1286I GDGCHC 4 cut(s) 32, 536, 1574, 1851
Bsp143I GATC 9 cut(s) 280, 289, 541, 574, 761, 865, 1411, 1988, 2143
BspACI CCGC 2 cut(s) 1681, 1974
BspANI GGCC 3 cut(s) 1810, 1956, 2030
BspCNI CTCAG 6 cut(s) 1282, 1350, 1419, 1606, 1705, 2393
BspDI ATCGAT 1 cut(s) 2408
BspHI TCATGA 1 cut(s) 250
BspLI GGNNCC 3 cut(s) 55, 1850, 2031
BspMAI CTGCAG 1 cut(s) 352
BspMI ACCTGC 1 cut(s) 119
BspOI GCTAGC 2 cut(s) 1325, 1908
BspPI GGATC 3 cut(s) 536, 582, 1983
BspQI GCTCTTC 1 cut(s) 524
BspTNI GGTCTC 1 cut(s) 2296
BsrDI GCAATG 1 cut(s) 2344
BsrFI RCCGGY 1 cut(s) 500
BsrI ACTGG 3 cut(s) 150, 1559, 2205
BssAI RCCGGY 1 cut(s) 500
BssECI CCNNGG 3 cut(s) 49, 160, 1447
BssMI GATC 9 cut(s) 280, 289, 541, 574, 761, 865, 1411, 1988, 2143
BssSI CACGAG 1 cut(s) 1141
BssT1I CCWWGG 2 cut(s) 49, 1447
Bst2BI CACGAG 1 cut(s) 1141
Bst2UI CCWGG 5 cut(s) 162, 552, 1236, 1958, 2370
Bst4CI ACNGT 4 cut(s) 317, 856, 1348, 2181
Bst6I CTCTTC 3 cut(s) 252, 524, 2292
BstBAI YACGTR 1 cut(s) 2225
BstC8I GCNNGC 5 cut(s) 937, 941, 1193, 1323, 1906
BstDEI CTNAG 9 cut(s) 493, 615, 793, 1290, 1337, 1406, 1614, 1713, 2380
BstEII GGTNACC 2 cut(s) 1442, 2344
BstF5I GGATG 6 cut(s) 329, 576, 733, 1180, 1269, 2190
BstHHI GCGC 1 cut(s) 896
BstKTI GATC 9 cut(s) 283, 292, 544, 577, 764, 868, 1414, 1991, 2146
BstMAI GTCTC 2 cut(s) 523, 2296
BstMBI GATC 9 cut(s) 280, 289, 541, 574, 761, 865, 1411, 1988, 2143
BstMWI GCNNNNNNNGC 1 cut(s) 1807
BstNI CCWGG 5 cut(s) 162, 552, 1236, 1958, 2370
BstNSI RCATGY 3 cut(s) 939, 2171, 2314
BstPI GGTNACC 2 cut(s) 1442, 2344
BstSCI CCNGG 5 cut(s) 160, 550, 1234, 1956, 2368
BstSFI CTRYAG 2 cut(s) 348, 852
BstSLI GKGCMC 1 cut(s) 1574
BstV1I GCAGC 5 cut(s) 1175, 1255, 1328, 1539, 2377
BstV2I GAAGAC 4 cut(s) 1470, 2252, 2296, 2418
BstX2I RGATCY 3 cut(s) 289, 541, 574
BstXI CCANNNNNNTGG 1 cut(s) 972
BstYI RGATCY 3 cut(s) 289, 541, 574
Bsu15I ATCGAT 1 cut(s) 2408
Bsu36I CCTNAGG 1 cut(s) 793
BsuRI GGCC 3 cut(s) 1810, 1956, 2030
BsuTUI ATCGAT 1 cut(s) 2408
BtsCI GGATG 6 cut(s) 329, 576, 733, 1180, 1269, 2190
BtsI GCAGTG 1 cut(s) 1033
BtsIMutI CAGTG 5 cut(s) 875, 1033, 1566, 1710, 2177
BveI ACCTGC 1 cut(s) 119
Cac8I GCNNGC 5 cut(s) 937, 941, 1193, 1323, 1906
CciI TCATGA 1 cut(s) 250
CfoI GCGC 1 cut(s) 896
Cfr10I RCCGGY 1 cut(s) 500
Cfr13I GGNCC 4 cut(s) 54, 1556, 1809, 2029
ClaI ATCGAT 1 cut(s) 2408
Csp6I GTAC 4 cut(s) 566, 953, 1146, 2093
CspAI ACCGGT 1 cut(s) 500
CspCI CAANNNNNGTGG 2 cut(s) 1986, 2021
CviQI GTAC 4 cut(s) 566, 953, 1146, 2093
DdeI CTNAG 9 cut(s) 493, 615, 793, 1290, 1337, 1406, 1614, 1713, 2380
DpnI GATC 9 cut(s) 282, 291, 543, 576, 763, 867, 1413, 1990, 2145
DpnII GATC 9 cut(s) 280, 289, 541, 574, 761, 865, 1411, 1988, 2143
DraI TTTAAA 1 cut(s) 270
DriI GACNNNNNGTC 1 cut(s) 90
Eam1104I CTCTTC 3 cut(s) 252, 524, 2292
Eam1105I GACNNNNNGTC 1 cut(s) 90
EarI CTCTTC 3 cut(s) 252, 524, 2292
Eco130I CCWWGG 2 cut(s) 49, 1447
Eco24I GRGCYC 1 cut(s) 1851
Eco31I GGTCTC 1 cut(s) 2296
Eco32I GATATC 2 cut(s) 671, 1168
Eco47I GGWCC 2 cut(s) 54, 1556
Eco57I CTGAAG 5 cut(s) 243, 1257, 1658, 2316, 2412
Eco81I CCTNAGG 1 cut(s) 793
Eco88I CYCGRG 2 cut(s) 772, 1842
Eco91I GGTNACC 2 cut(s) 1442, 2344
EcoO109I RGGNCCY 1 cut(s) 54
EcoO65I GGTNACC 2 cut(s) 1442, 2344
EcoRI GAATTC 4 cut(s) 976, 1226, 1618, 1742
EcoRII CCWGG 5 cut(s) 160, 550, 1234, 1956, 2368
EcoRV GATATC 2 cut(s) 671, 1168
EcoT14I CCWWGG 2 cut(s) 49, 1447
EcoT22I ATGCAT 2 cut(s) 2173, 2418
EcoT38I GRGCYC 1 cut(s) 1851
ErhI CCWWGG 2 cut(s) 49, 1447
FalI AAGNNNNNCTT 4 cut(s) 276, 308, 1431, 1463
FaqI GGGAC 4 cut(s) 67, 774, 1374, 2283
FauNDI CATATG 2 cut(s) 1153, 2017
FbaI TGATCA 1 cut(s) 280
FblI GTMKAC 2 cut(s) 822, 2392
Fnu4HI GCNGC 5 cut(s) 1189, 1269, 1342, 1528, 2366
FokI GGATG 6 cut(s) 316, 583, 740, 1187, 1276, 2197
FriOI GRGCYC 1 cut(s) 1851
Fsp4HI GCNGC 5 cut(s) 1189, 1269, 1342, 1528, 2366
GlaI GCGC 1 cut(s) 895
GluI GCNGC 5 cut(s) 1189, 1269, 1342, 1528, 2366
GsaI CCCAGC 2 cut(s) 808, 1541
GsuI CTGGAG 2 cut(s) 534, 1444
HaeIII GGCC 3 cut(s) 1810, 1956, 2030
HapII CCGG 1 cut(s) 501
HhaI GCGC 1 cut(s) 896
Hin6I GCGC 1 cut(s) 894
HinP1I GCGC 1 cut(s) 894
HincII GTYRAC 1 cut(s) 823
HindII GTYRAC 1 cut(s) 823
HindIII AAGCTT 3 cut(s) 479, 1694, 1759
HpaII CCGG 1 cut(s) 501
HphI GGTGA 8 cut(s) 122, 176, 687, 1853, 1988, 2138, 2394, 2434
Hpy166II GTNNAC 4 cut(s) 680, 823, 1146, 2393
Hpy8I GTNNAC 4 cut(s) 680, 823, 1146, 2393
HpyAV CCTTC 6 cut(s) 55, 308, 462, 601, 1538, 2168
HpyCH4III ACNGT 4 cut(s) 317, 856, 1348, 2181
HpyCH4IV ACGT 2 cut(s) 2219, 2224
HpyF10VI GCNNNNNNNGC 1 cut(s) 1807
HpyF3I CTNAG 9 cut(s) 493, 615, 793, 1290, 1337, 1406, 1614, 1713, 2380
HpySE526I ACGT 2 cut(s) 2219, 2224
HspAI GCGC 1 cut(s) 894
Ksp22I TGATCA 1 cut(s) 280
Kzo9I GATC 9 cut(s) 280, 289, 541, 574, 761, 865, 1411, 1988, 2143
LguI GCTCTTC 1 cut(s) 524
LmnI GCTCC 4 cut(s) 135, 553, 611, 1854
Lsp1109I GCAGC 5 cut(s) 1175, 1255, 1328, 1539, 2377
LweI GCATC 2 cut(s) 2175, 2403
MaeII ACGT 2 cut(s) 2219, 2224
MaeIII GTNAC 7 cut(s) 211, 496, 655, 1442, 1502, 2220, 2344
MalI GATC 9 cut(s) 282, 291, 543, 576, 763, 867, 1413, 1990, 2145
MboI GATC 9 cut(s) 280, 289, 541, 574, 761, 865, 1411, 1988, 2143
MflI RGATCY 3 cut(s) 289, 541, 574
MhlI GDGCHC 4 cut(s) 32, 536, 1574, 1851
MlyI GAGTC 6 cut(s) 79, 796, 1006, 1862, 2398, 2468
MmeI TCCRAC 2 cut(s) 467, 2436
Mph1103I ATGCAT 2 cut(s) 2173, 2418
MspA1I CMGCKG 1 cut(s) 1341
MspI CCGG 1 cut(s) 501
MspR9I CCNGG 5 cut(s) 162, 552, 1236, 1958, 2370
MvaI CCWGG 5 cut(s) 162, 552, 1236, 1958, 2370
MwoI GCNNNNNNNGC 1 cut(s) 1807
NdeI CATATG 2 cut(s) 1153, 2017
NdeII GATC 9 cut(s) 280, 289, 541, 574, 761, 865, 1411, 1988, 2143
NheI GCTAGC 2 cut(s) 1321, 1904
NlaIV GGNNCC 3 cut(s) 55, 1850, 2031
NmuCI GTSAC 1 cut(s) 211
NsiI ATGCAT 2 cut(s) 2173, 2418
NspI RCATGY 3 cut(s) 939, 2171, 2314
PaeI GCATGC 1 cut(s) 939
PaeR7I CTCGAG 2 cut(s) 772, 1842
PagI TCATGA 1 cut(s) 250
PaqCI CACCTGC 1 cut(s) 119
PciI ACATGT 1 cut(s) 2310
PciSI GCTCTTC 1 cut(s) 524
PfeI GAWTC 7 cut(s) 150, 254, 355, 377, 2130, 2228, 2399
PfoI TCCNGGA 1 cut(s) 550
PinAI ACCGGT 1 cut(s) 500
PkrI GCNGC 5 cut(s) 1190, 1270, 1343, 1529, 2367
PleI GAGTC 6 cut(s) 79, 795, 1006, 1862, 2397, 2467
PpsI GAGTC 6 cut(s) 79, 795, 1006, 1862, 2397, 2467
Ppu21I YACGTR 1 cut(s) 2225
PpuMI RGGWCCY 1 cut(s) 54
PscI ACATGT 1 cut(s) 2310
PshBI ATTAAT 3 cut(s) 1367, 1577, 2207
Psp5II RGGWCCY 1 cut(s) 54
Psp6I CCWGG 5 cut(s) 160, 550, 1234, 1956, 2368
PspEI GGTNACC 2 cut(s) 1442, 2344
PspFI CCCAGC 2 cut(s) 804, 1537
PspGI CCWGG 5 cut(s) 160, 550, 1234, 1956, 2368
PspN4I GGNNCC 3 cut(s) 55, 1850, 2031
PspPI GGNCC 4 cut(s) 54, 1556, 1809, 2029
PspPPI RGGWCCY 1 cut(s) 54
PspXI VCTCGAGB 1 cut(s) 1842
PsrI GAACNNNNNNTAC 2 cut(s) 2376, 2408
PstI CTGCAG 1 cut(s) 352
PsuI RGATCY 3 cut(s) 289, 541, 574
PvuII CAGCTG 1 cut(s) 1341
RsaI GTAC 4 cut(s) 567, 954, 1147, 2094
RsaNI GTAC 4 cut(s) 566, 953, 1146, 2093
SalI GTCGAC 1 cut(s) 821
SapI GCTCTTC 1 cut(s) 524
SatI GCNGC 5 cut(s) 1189, 1269, 1342, 1528, 2366
Sau3AI GATC 9 cut(s) 280, 289, 541, 574, 761, 865, 1411, 1988, 2143
Sau96I GGNCC 4 cut(s) 54, 1556, 1809, 2029
SchI GAGTC 6 cut(s) 79, 796, 1006, 1862, 2398, 2468
ScrFI CCNGG 5 cut(s) 162, 552, 1236, 1958, 2370
SduI GDGCHC 4 cut(s) 32, 536, 1574, 1851
SfaNI GCATC 2 cut(s) 2175, 2403
SfcI CTRYAG 2 cut(s) 348, 852
Sfr274I CTCGAG 2 cut(s) 772, 1842
SinI GGWCC 2 cut(s) 54, 1556
SlaI CTCGAG 2 cut(s) 772, 1842
SmlI CTYRAG 5 cut(s) 58, 772, 1106, 1842, 1870
SmoI CTYRAG 5 cut(s) 58, 772, 1106, 1842, 1870
SphI GCATGC 1 cut(s) 939
SsiI CCGC 2 cut(s) 1681, 1974
SspI AATATT 1 cut(s) 1912
StyD4I CCNGG 5 cut(s) 160, 550, 1234, 1956, 2368
StyI CCWWGG 2 cut(s) 49, 1447
TaaI ACNGT 4 cut(s) 317, 856, 1348, 2181
TaiI ACGT 2 cut(s) 2222, 2227
TaqI TCGA 6 cut(s) 773, 822, 1803, 1843, 1920, 2408
TatI WGTACW 1 cut(s) 2092
TfiI GAWTC 7 cut(s) 150, 254, 355, 377, 2130, 2228, 2399
TscAI CASTG 5 cut(s) 875, 1033, 1566, 1717, 2184
TseFI GTSAC 1 cut(s) 211
TseI GCWGC 5 cut(s) 1188, 1268, 1341, 1527, 2365
Tsp45I GTSAC 1 cut(s) 211
TspGWI ACGGA 3 cut(s) 583, 1276, 2401
TspRI CASTG 5 cut(s) 875, 1033, 1566, 1717, 2184
VpaK11BI GGWCC 2 cut(s) 54, 1556
VspI ATTAAT 3 cut(s) 1367, 1577, 2207
XapI RAATTY 7 cut(s) 594, 976, 1045, 1226, 1618, 1742, 2052
XceI RCATGY 3 cut(s) 939, 2171, 2314
XcmI CCANNNNNNNNNTGG 2 cut(s) 1759, 2452
XhoI CTCGAG 2 cut(s) 772, 1842
XmaJI CCTAGG 1 cut(s) 49
XmiI GTMKAC 2 cut(s) 822, 2392
Zsp2I ATGCAT 2 cut(s) 2173, 2418
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.