Serine threonine-protein kinase
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 1370 |
| AarI | CACCTGC | 1 cut(s) | 119 |
| Acc36I | ACCTGC | 1 cut(s) | 119 |
| Acc65I | GGTACC | 1 cut(s) | 513 |
| AccB1I | GGYRCC | 1 cut(s) | 513 |
| AccII | CGCG | 1 cut(s) | 71 |
| AccIII | TCCGGA | 1 cut(s) | 1610 |
| AciI | CCGC | 2 cut(s) | 69, 1669 |
| AclWI | GGATC | 3 cut(s) | 536, 582, 1915 |
| AcsI | RAATTY | 6 cut(s) | 334, 976, 1162, 1606, 1730, 2040 |
| AcuI | CTGAAG | 4 cut(s) | 243, 1260, 1646, 2253 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 551, 700, 1593 |
| AflIII | ACRYGT | 3 cut(s) | 759, 2013, 2298 |
| AhdI | GACNNNNNGTC | 2 cut(s) | 23, 293 |
| AhlI | ACTAGT | 1 cut(s) | 902 |
| AjiI | CACGTC | 1 cut(s) | 762 |
| AjnI | CCWGG | 5 cut(s) | 160, 550, 631, 1851, 2356 |
| AjuI | GAANNNNNNNTTGG | 6 cut(s) | 231, 263, 465, 497, 1524, 1556 |
| AleI | CACNNNNGTG | 2 cut(s) | 818, 2435 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 2364, 2396 |
| Alw21I | GWGCWC | 2 cut(s) | 536, 1307 |
| Alw26I | GTCTC | 4 cut(s) | 99, 206, 925, 2022 |
| AlwI | GGATC | 3 cut(s) | 536, 582, 1915 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 800 |
| Ama87I | CYCGRG | 1 cut(s) | 772 |
| Aor13HI | TCCGGA | 1 cut(s) | 1610 |
| AoxI | GGCC | 3 cut(s) | 646, 1796, 1942 |
| ApeKI | GCWGC | 4 cut(s) | 1026, 1271, 1515, 2353 |
| ApoI | RAATTY | 6 cut(s) | 334, 976, 1162, 1606, 1730, 2040 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 2423 |
| Asp718I | GGTACC | 1 cut(s) | 513 |
| AspS9I | GGNCC | 1 cut(s) | 1544 |
| AsuHPI | GGTGA | 4 cut(s) | 122, 1841, 2126, 2382 |
| AsuII | TTCGAA | 1 cut(s) | 1601 |
| AsuNHI | GCTAGC | 1 cut(s) | 1892 |
| AvaI | CYCGRG | 1 cut(s) | 772 |
| AvaII | GGWCC | 1 cut(s) | 1544 |
| BanI | GGYRCC | 1 cut(s) | 513 |
| BbsI | GAAGAC | 4 cut(s) | 22, 1449, 1458, 2240 |
| Bbv12I | GWGCWC | 2 cut(s) | 536, 1307 |
| BbvI | GCAGC | 4 cut(s) | 1038, 1283, 1527, 2365 |
| BccI | CCATC | 9 cut(s) | 395, 565, 799, 989, 1031, 1261, 1280, 2030, 2401 |
| BceAI | ACGGC | 3 cut(s) | 661, 1167, 1571 |
| BciT130I | CCWGG | 5 cut(s) | 162, 552, 633, 1853, 2358 |
| BclI | TGATCA | 2 cut(s) | 1768, 1966 |
| BcoDI | GTCTC | 4 cut(s) | 99, 206, 925, 2022 |
| BcuI | ACTAGT | 1 cut(s) | 902 |
| BfaI | CTAG | 9 cut(s) | 293, 627, 903, 1487, 1830, 1860, 1893, 1946, 2087 |
| BfmI | CTRYAG | 5 cut(s) | 348, 852, 1347, 1623, 2433 |
| BfuAI | ACCTGC | 1 cut(s) | 119 |
| BisI | GCNGC | 4 cut(s) | 1027, 1272, 1516, 2354 |
| BlpI | GCTNAGC | 1 cut(s) | 2321 |
| BlsI | GCNGC | 4 cut(s) | 1028, 1273, 1517, 2355 |
| Bme1390I | CCNGG | 5 cut(s) | 162, 552, 633, 1853, 2358 |
| Bme18I | GGWCC | 1 cut(s) | 1544 |
| BmeRI | GACNNNNNGTC | 2 cut(s) | 23, 293 |
| BmeT110I | CYCGRG | 1 cut(s) | 772 |
| BmgBI | CACGTC | 1 cut(s) | 762 |
| BmgT120I | GGNCC | 1 cut(s) | 1544 |
| BmiI | GGNNCC | 1 cut(s) | 515 |
| BmrFI | CCNGG | 5 cut(s) | 162, 552, 633, 1853, 2358 |
| BmrI | ACTGGG | 2 cut(s) | 155, 1065 |
| BmtI | GCTAGC | 1 cut(s) | 1896 |
| BmuI | ACTGGG | 2 cut(s) | 155, 1065 |
| BoxI | GACNNNNGTC | 1 cut(s) | 634 |
| BpiI | GAAGAC | 4 cut(s) | 22, 1449, 1458, 2240 |
| BpmI | CTGGAG | 1 cut(s) | 534 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1030 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 2321 |
| Bpu14I | TTCGAA | 1 cut(s) | 1601 |
| BpuEI | CTTGAG | 2 cut(s) | 31, 1094 |
| BsaAI | YACGTR | 1 cut(s) | 1803 |
| BsaJI | CCNNGG | 3 cut(s) | 160, 632, 1435 |
| BsaWI | WCCGGW | 2 cut(s) | 1238, 1610 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 70, 100, 2326, 2356 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 551, 700, 1593 |
| Bse1I | ACTGG | 7 cut(s) | 150, 359, 505, 1060, 1192, 1799, 2193 |
| Bse3DI | GCAATG | 2 cut(s) | 1126, 1903 |
| BseAI | TCCGGA | 1 cut(s) | 1610 |
| BseBI | CCWGG | 5 cut(s) | 162, 552, 633, 1853, 2358 |
| BseDI | CCNNGG | 3 cut(s) | 160, 632, 1435 |
| BseGI | GGATG | 4 cut(s) | 576, 733, 1272, 2125 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 551, 700, 1593 |
| BseMI | GCAATG | 2 cut(s) | 1126, 1903 |
| BseMII | CTCAG | 5 cut(s) | 201, 270, 1044, 1420, 2312 |
| BseNI | ACTGG | 7 cut(s) | 150, 359, 505, 1060, 1192, 1799, 2193 |
| BseRI | GAGGAG | 1 cut(s) | 544 |
| BseXI | GCAGC | 4 cut(s) | 1038, 1283, 1527, 2365 |
| BseYI | CCCAGC | 1 cut(s) | 1525 |
| BsgI | GTGCAG | 1 cut(s) | 786 |
| Bsh1236I | CGCG | 1 cut(s) | 71 |
| BshFI | GGCC | 3 cut(s) | 648, 1798, 1944 |
| BshNI | GGYRCC | 1 cut(s) | 513 |
| BsiHKAI | GWGCWC | 2 cut(s) | 536, 1307 |
| BsiHKCI | CYCGRG | 1 cut(s) | 772 |
| BsiSI | CCGG | 2 cut(s) | 1239, 1611 |
| BslFI | GGGAC | 3 cut(s) | 503, 1791, 2366 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 551, 700, 1593 |
| BsmAI | GTCTC | 4 cut(s) | 99, 206, 925, 2022 |
| BsmBI | CGTCTC | 1 cut(s) | 99 |
| BsmFI | GGGAC | 3 cut(s) | 503, 1791, 2366 |
| BsmI | GAATGC | 1 cut(s) | 1121 |
| BsnI | GGCC | 3 cut(s) | 648, 1798, 1944 |
| BsoBI | CYCGRG | 1 cut(s) | 772 |
| Bsp119I | TTCGAA | 1 cut(s) | 1601 |
| Bsp1286I | GDGCHC | 2 cut(s) | 536, 1307 |
| Bsp13I | TCCGGA | 1 cut(s) | 1610 |
| Bsp1407I | TGTACA | 1 cut(s) | 1551 |
| Bsp143I | GATC | 9 cut(s) | 95, 541, 574, 865, 1768, 1920, 1966, 2131, 2439 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 2321 |
| BspACI | CCGC | 2 cut(s) | 69, 1669 |
| BspANI | GGCC | 3 cut(s) | 648, 1798, 1944 |
| BspCNI | CTCAG | 5 cut(s) | 202, 271, 1043, 1419, 2313 |
| BspEI | TCCGGA | 1 cut(s) | 1610 |
| BspFNI | CGCG | 1 cut(s) | 71 |
| BspLI | GGNNCC | 1 cut(s) | 515 |
| BspMAI | CTGCAG | 3 cut(s) | 352, 856, 1351 |
| BspMI | ACCTGC | 1 cut(s) | 119 |
| BspOI | GCTAGC | 1 cut(s) | 1896 |
| BspPI | GGATC | 3 cut(s) | 536, 582, 1915 |
| BspT104I | TTCGAA | 1 cut(s) | 1601 |
| BspT107I | GGYRCC | 1 cut(s) | 513 |
| BsrDI | GCAATG | 2 cut(s) | 1126, 1903 |
| BsrGI | TGTACA | 1 cut(s) | 1551 |
| BsrI | ACTGG | 7 cut(s) | 150, 359, 505, 1060, 1192, 1799, 2193 |
| BssECI | CCNNGG | 3 cut(s) | 160, 632, 1435 |
| BssMI | GATC | 9 cut(s) | 95, 541, 574, 865, 1768, 1920, 1966, 2131, 2439 |
| BssT1I | CCWWGG | 1 cut(s) | 1435 |
| Bst2UI | CCWGG | 5 cut(s) | 162, 552, 633, 1853, 2358 |
| Bst4CI | ACNGT | 3 cut(s) | 110, 217, 2437 |
| Bst6I | CTCTTC | 1 cut(s) | 1701 |
| BstAUI | TGTACA | 1 cut(s) | 1551 |
| BstBAI | YACGTR | 1 cut(s) | 1803 |
| BstBI | TTCGAA | 1 cut(s) | 1601 |
| BstC8I | GCNNGC | 5 cut(s) | 6, 691, 937, 1024, 1894 |
| BstDEI | CTNAG | 8 cut(s) | 184, 210, 279, 615, 1030, 1406, 1926, 2321 |
| BstEII | GGTNACC | 1 cut(s) | 1208 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 549 |
| BstF5I | GGATG | 4 cut(s) | 576, 733, 1272, 2125 |
| BstFNI | CGCG | 1 cut(s) | 71 |
| BstKTI | GATC | 9 cut(s) | 98, 544, 577, 868, 1771, 1923, 1969, 2134, 2442 |
| BstMAI | GTCTC | 4 cut(s) | 99, 206, 925, 2022 |
| BstMBI | GATC | 9 cut(s) | 95, 541, 574, 865, 1768, 1920, 1966, 2131, 2439 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 860, 1795 |
| BstNI | CCWGG | 5 cut(s) | 162, 552, 633, 1853, 2358 |
| BstNSI | RCATGY | 4 cut(s) | 939, 2017, 2159, 2302 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 634 |
| BstPI | GGTNACC | 1 cut(s) | 1208 |
| BstSCI | CCNGG | 5 cut(s) | 160, 550, 631, 1851, 2356 |
| BstSFI | CTRYAG | 5 cut(s) | 348, 852, 1347, 1623, 2433 |
| BstUI | CGCG | 1 cut(s) | 71 |
| BstV1I | GCAGC | 4 cut(s) | 1038, 1283, 1527, 2365 |
| BstV2I | GAAGAC | 4 cut(s) | 22, 1449, 1458, 2240 |
| BstX2I | RGATCY | 1 cut(s) | 541 |
| BstXI | CCANNNNNNTGG | 3 cut(s) | 639, 972, 1817 |
| BstYI | RGATCY | 1 cut(s) | 541 |
| BsuRI | GGCC | 3 cut(s) | 648, 1798, 1944 |
| BtrI | CACGTC | 1 cut(s) | 762 |
| BtsCI | GGATG | 4 cut(s) | 576, 733, 1272, 2125 |
| BtsI | GCAGTG | 3 cut(s) | 798, 916, 2332 |
| BveI | ACCTGC | 1 cut(s) | 119 |
| Cac8I | GCNNGC | 5 cut(s) | 6, 691, 937, 1024, 1894 |
| CaiI | CAGNNNCTG | 1 cut(s) | 800 |
| Cfr13I | GGNCC | 1 cut(s) | 1544 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 1974, 2009 |
| DdeI | CTNAG | 8 cut(s) | 184, 210, 279, 615, 1030, 1406, 1926, 2321 |
| DpnI | GATC | 9 cut(s) | 97, 543, 576, 867, 1770, 1922, 1968, 2133, 2441 |
| DpnII | GATC | 9 cut(s) | 95, 541, 574, 865, 1768, 1920, 1966, 2131, 2439 |
| DriI | GACNNNNNGTC | 2 cut(s) | 23, 293 |
| Eam1104I | CTCTTC | 1 cut(s) | 1701 |
| Eam1105I | GACNNNNNGTC | 2 cut(s) | 23, 293 |
| EarI | CTCTTC | 1 cut(s) | 1701 |
| Eco130I | CCWWGG | 1 cut(s) | 1435 |
| Eco32I | GATATC | 2 cut(s) | 665, 1171 |
| Eco47I | GGWCC | 1 cut(s) | 1544 |
| Eco57I | CTGAAG | 4 cut(s) | 243, 1260, 1646, 2253 |
| Eco88I | CYCGRG | 1 cut(s) | 772 |
| Eco91I | GGTNACC | 1 cut(s) | 1208 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 549 |
| EcoO65I | GGTNACC | 1 cut(s) | 1208 |
| EcoRI | GAATTC | 3 cut(s) | 976, 1606, 1730 |
| EcoRII | CCWGG | 5 cut(s) | 160, 550, 631, 1851, 2356 |
| EcoRV | GATATC | 2 cut(s) | 665, 1171 |
| EcoT14I | CCWWGG | 1 cut(s) | 1435 |
| EcoT22I | ATGCAT | 1 cut(s) | 2161 |
| ErhI | CCWWGG | 1 cut(s) | 1435 |
| Esp3I | CGTCTC | 1 cut(s) | 99 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 1419, 1451 |
| FaqI | GGGAC | 3 cut(s) | 503, 1791, 2366 |
| FauNDI | CATATG | 3 cut(s) | 929, 1156, 2305 |
| FbaI | TGATCA | 2 cut(s) | 1768, 1966 |
| Fnu4HI | GCNGC | 4 cut(s) | 1027, 1272, 1516, 2354 |
| FokI | GGATG | 4 cut(s) | 583, 740, 1279, 2112 |
| Fsp4HI | GCNGC | 4 cut(s) | 1027, 1272, 1516, 2354 |
| FspBI | CTAG | 9 cut(s) | 293, 627, 903, 1487, 1830, 1860, 1893, 1946, 2087 |
| GluI | GCNGC | 4 cut(s) | 1027, 1272, 1516, 2354 |
| GsaI | CCCAGC | 1 cut(s) | 1529 |
| GsuI | CTGGAG | 1 cut(s) | 534 |
| HaeIII | GGCC | 3 cut(s) | 648, 1798, 1944 |
| HapII | CCGG | 2 cut(s) | 1239, 1611 |
| HincII | GTYRAC | 1 cut(s) | 424 |
| HindII | GTYRAC | 1 cut(s) | 424 |
| HindIII | AAGCTT | 2 cut(s) | 479, 1747 |
| HpaI | GTTAAC | 1 cut(s) | 424 |
| HpaII | CCGG | 2 cut(s) | 1239, 1611 |
| HphI | GGTGA | 4 cut(s) | 122, 1841, 2126, 2382 |
| Hpy166II | GTNNAC | 4 cut(s) | 424, 823, 1482, 1552 |
| Hpy188I | TCNGA | 6 cut(s) | 994, 1279, 1409, 1936, 2059, 2233 |
| Hpy8I | GTNNAC | 4 cut(s) | 424, 823, 1482, 1552 |
| Hpy99I | CGWCG | 2 cut(s) | 582, 2209 |
| HpyAV | CCTTC | 7 cut(s) | 55, 265, 308, 659, 937, 1526, 2156 |
| HpyCH4III | ACNGT | 3 cut(s) | 110, 217, 2437 |
| HpyCH4IV | ACGT | 4 cut(s) | 761, 1802, 2003, 2207 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 860, 1795 |
| HpyF3I | CTNAG | 8 cut(s) | 184, 210, 279, 615, 1030, 1406, 1926, 2321 |
| HpySE526I | ACGT | 4 cut(s) | 761, 1802, 2003, 2207 |
| Kpn2I | TCCGGA | 1 cut(s) | 1610 |
| KpnI | GGTACC | 1 cut(s) | 517 |
| Ksp22I | TGATCA | 2 cut(s) | 1768, 1966 |
| KspAI | GTTAAC | 1 cut(s) | 424 |
| Kzo9I | GATC | 9 cut(s) | 95, 541, 574, 865, 1768, 1920, 1966, 2131, 2439 |
| LmnI | GCTCC | 4 cut(s) | 35, 531, 553, 611 |
| Lsp1109I | GCAGC | 4 cut(s) | 1038, 1283, 1527, 2365 |
| MaeI | CTAG | 9 cut(s) | 293, 627, 903, 1487, 1830, 1860, 1893, 1946, 2087 |
| MaeII | ACGT | 4 cut(s) | 761, 1802, 2003, 2207 |
| MaeIII | GTNAC | 8 cut(s) | 274, 455, 496, 598, 1208, 1420, 1461, 2009 |
| MalI | GATC | 9 cut(s) | 97, 543, 576, 867, 1770, 1922, 1968, 2133, 2441 |
| MboI | GATC | 9 cut(s) | 95, 541, 574, 865, 1768, 1920, 1966, 2131, 2439 |
| MflI | RGATCY | 1 cut(s) | 541 |
| MhlI | GDGCHC | 2 cut(s) | 536, 1307 |
| MlyI | GAGTC | 2 cut(s) | 1428, 1850 |
| MmeI | TCCRAC | 1 cut(s) | 1978 |
| Mph1103I | ATGCAT | 1 cut(s) | 2161 |
| MroI | TCCGGA | 1 cut(s) | 1610 |
| MroXI | GAANNNNTTC | 1 cut(s) | 2423 |
| MseI | TTAA | 6 cut(s) | 38, 423, 1062, 1865, 2142, 2240 |
| MslI | CAYNNNNRTG | 4 cut(s) | 764, 818, 1220, 2435 |
| MspI | CCGG | 2 cut(s) | 1239, 1611 |
| MspR9I | CCNGG | 5 cut(s) | 162, 552, 633, 1853, 2358 |
| Mva1269I | GAATGC | 1 cut(s) | 1121 |
| MvaI | CCWGG | 5 cut(s) | 162, 552, 633, 1853, 2358 |
| MvnI | CGCG | 1 cut(s) | 71 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 860, 1795 |
| NdeI | CATATG | 3 cut(s) | 929, 1156, 2305 |
| NdeII | GATC | 9 cut(s) | 95, 541, 574, 865, 1768, 1920, 1966, 2131, 2439 |
| NheI | GCTAGC | 1 cut(s) | 1892 |
| NlaIV | GGNNCC | 1 cut(s) | 515 |
| NmuCI | GTSAC | 3 cut(s) | 274, 455, 1420 |
| NsiI | ATGCAT | 1 cut(s) | 2161 |
| NspI | RCATGY | 4 cut(s) | 939, 2017, 2159, 2302 |
| NspV | TTCGAA | 1 cut(s) | 1601 |
| OliI | CACNNNNGTG | 2 cut(s) | 818, 2435 |
| PaeI | GCATGC | 1 cut(s) | 939 |
| PaeR7I | CTCGAG | 1 cut(s) | 772 |
| PaqCI | CACCTGC | 1 cut(s) | 119 |
| PciI | ACATGT | 2 cut(s) | 2013, 2298 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 574 |
| PctI | GAATGC | 1 cut(s) | 1121 |
| PdmI | GAANNNNTTC | 1 cut(s) | 2423 |
| PfoI | TCCNGGA | 1 cut(s) | 550 |
| PkrI | GCNGC | 4 cut(s) | 1028, 1273, 1517, 2355 |
| PleI | GAGTC | 2 cut(s) | 1427, 1850 |
| PpsI | GAGTC | 2 cut(s) | 1427, 1850 |
| Ppu21I | YACGTR | 1 cut(s) | 1803 |
| PscI | ACATGT | 2 cut(s) | 2013, 2298 |
| PshAI | GACNNNNGTC | 1 cut(s) | 634 |
| PsiI | TTATAA | 1 cut(s) | 1370 |
| Psp6I | CCWGG | 5 cut(s) | 160, 550, 631, 1851, 2356 |
| PspEI | GGTNACC | 1 cut(s) | 1208 |
| PspFI | CCCAGC | 1 cut(s) | 1525 |
| PspGI | CCWGG | 5 cut(s) | 160, 550, 631, 1851, 2356 |
| PspN4I | GGNNCC | 1 cut(s) | 515 |
| PspPI | GGNCC | 1 cut(s) | 1544 |
| PstI | CTGCAG | 3 cut(s) | 352, 856, 1351 |
| PstNI | CAGNNNCTG | 1 cut(s) | 800 |
| PsuI | RGATCY | 1 cut(s) | 541 |
| RseI | CAYNNNNRTG | 4 cut(s) | 764, 818, 1220, 2435 |
| SaqAI | TTAA | 6 cut(s) | 38, 423, 1062, 1865, 2142, 2240 |
| SatI | GCNGC | 4 cut(s) | 1027, 1272, 1516, 2354 |
| Sau3AI | GATC | 9 cut(s) | 95, 541, 574, 865, 1768, 1920, 1966, 2131, 2439 |
| Sau96I | GGNCC | 1 cut(s) | 1544 |
| SchI | GAGTC | 2 cut(s) | 1428, 1850 |
| ScrFI | CCNGG | 5 cut(s) | 162, 552, 633, 1853, 2358 |
| SduI | GDGCHC | 2 cut(s) | 536, 1307 |
| SfcI | CTRYAG | 5 cut(s) | 348, 852, 1347, 1623, 2433 |
| Sfr274I | CTCGAG | 1 cut(s) | 772 |
| SfuI | TTCGAA | 1 cut(s) | 1601 |
| SinI | GGWCC | 1 cut(s) | 1544 |
| SlaI | CTCGAG | 1 cut(s) | 772 |
| SmiMI | CAYNNNNRTG | 4 cut(s) | 764, 818, 1220, 2435 |
| SmlI | CTYRAG | 3 cut(s) | 10, 772, 1109 |
| SmoI | CTYRAG | 3 cut(s) | 10, 772, 1109 |
| SpeI | ACTAGT | 1 cut(s) | 902 |
| SphI | GCATGC | 1 cut(s) | 939 |
| SsiI | CCGC | 2 cut(s) | 69, 1669 |
| SspMI | CTAG | 9 cut(s) | 293, 627, 903, 1487, 1830, 1860, 1893, 1946, 2087 |
| StyD4I | CCNGG | 5 cut(s) | 160, 550, 631, 1851, 2356 |
| StyI | CCWWGG | 1 cut(s) | 1435 |
| TaaI | ACNGT | 3 cut(s) | 110, 217, 2437 |
| TaiI | ACGT | 4 cut(s) | 764, 1805, 2006, 2210 |
| TaqI | TCGA | 5 cut(s) | 94, 178, 773, 1601, 2442 |
| TatI | WGTACW | 2 cut(s) | 744, 1551 |
| Tru1I | TTAA | 6 cut(s) | 38, 423, 1062, 1865, 2142, 2240 |
| Tru9I | TTAA | 6 cut(s) | 38, 423, 1062, 1865, 2142, 2240 |
| TseFI | GTSAC | 3 cut(s) | 274, 455, 1420 |
| TseI | GCWGC | 4 cut(s) | 1026, 1271, 1515, 2353 |
| Tsp45I | GTSAC | 3 cut(s) | 274, 455, 1420 |
| TspGWI | ACGGA | 2 cut(s) | 583, 1279 |
| VpaK11BI | GGWCC | 1 cut(s) | 1544 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 549 |
| XapI | RAATTY | 6 cut(s) | 334, 976, 1162, 1606, 1730, 2040 |
| XbaI | TCTAGA | 2 cut(s) | 626, 1859 |
| XceI | RCATGY | 4 cut(s) | 939, 2017, 2159, 2302 |
| XcmI | CCANNNNNNNNNTGG | 2 cut(s) | 1747, 1817 |
| XhoI | CTCGAG | 1 cut(s) | 772 |
| XmnI | GAANNNNTTC | 1 cut(s) | 2423 |
| XspI | CTAG | 9 cut(s) | 293, 627, 903, 1487, 1830, 1860, 1893, 1946, 2087 |
| Zsp2I | ATGCAT | 1 cut(s) | 2161 |