RLG00000032968

divergent subfamily of APPLE domains

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0000207
Physical Location & Seq
Reverse (-)
21520555 .. 21524177
3623 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000032968

Sequence Viewer

Length: 2202 bp
ATGAAAACCCTTTTTGTGTGCTTGTTCTTATTTTCCTTCCTAAGAATCTCAAATACGCTGGAGTCTATCACTCCAAGTCAATTTATTAAAGATGGTCAAACTCTGGTTTCAGCAGGTGGAAGCTATGAACTGGGATTCTTTAGTCCCGGTAAAGTGAAAGGCAGATACTTGGGAATATGGTACACCTTTTCTACTGAGACAGTTGTATGGGTAGCTAACAGAGAAATACCACTTAATGATTCTTTAGGAGTTTTAAAGGTCACTGATCAGGGAATTTTAGTCCTTCTCAATAGCTCAAATGGCACTGTATGGTCATCCAATTCATCAAGAACTGCAGGGAATCCAGTCTCGCAACTCTTGGATTCGGGGAATCTTGTTGTGAAAGATGGAAATGAAACCAATCCTGATAACTTCATGTGGCAGAGTTTTGATTATCCTTGTGATACACGCCTGCCAGAAATGAAGCTTGGTTGGAATTTAATTTTTATTATGAAGGGAGCTAAGATACTGACTAGAGCTGGGACATGGAACGGCATTGAATTAACTGGATATCAAGGAAAGCCAAATCCAGTAGCGGTTTTTGAGTTTGTGTTGACTAAGGATGAAGTCTATTATGAGTACACACTCCTCAACCGGTCAATGTTTGCAAGATATGTATTAAACCCATATGGCATTGCACAGTGGCTTACTTGGACAGACCACTCGCGTAGTTGGGAACCTTTCATCTCAACTCAAGCAGATCAGTGTGAAAACTATGCCTATTGTGGTGCTAATGCTAGATGTAATGTCAGTAACGCTCCTGTATGTACATGCTTGAAAGGGTTTGTACCAAAATCTCCGAAAGATTGGAACTCGACAAATTGGTCTGGGGGATGTGTTCGGGAGACTCCATTAGCTTGCAGCTCTACAGATGGCTTCTCAAAGTACAGTAACTTTAAATTGCCAGACACATCTTCTTCCTGGTATGACAAAAGCAGGACCCTCAAGGAGTGCCAGGGATTATGTTTGGGAAACTGTTCGTGTACTGCATATGCAAATTTAGATGTCAGGGAAGGTGGAAGTGGCTGCTTGCTTTGGTTTGGGAACCTCAATGACATTAGAGAATTCACCTCTGATTCTCAAGACCTCTTTATACGGCTGGCTGCCTCAGACCTAGATCCTATTGTGAAAAAAAGCAAGTCCAGCAAGAAAAAGCTAGCAGGAATTCTGATCGGCTCATCTGTATTTCTTGTGGGAATGCTAATAGTTGGGTTCCTTGTGTATAAACGGAAGAGGAAACTCAGAAAGCAAGGTGAGGGAACATTGATTGGAGGAGAGGAAATTGCAGTAAAGAGGCTCTCAAAAAATTCTGGACAGGGATTGAGAGAGTTCAAAAACGAAGTTCTACTGATAGCCAAACTTCAGCATAGGAATCTTGTAAAGCTTCTTGGTTGTTGCATTCAAGAAGAAGAAGAAATTTTAGTATATGAATTCATGCCTAACAGAAGCTTGGACTTCTTTATATTTGATCAAGAAAGAGAGAAGTTGCTCGACTGGCCCACATGCTTCCATATTATTGAGGGAATTGCTCGAGGGCTTCTTTATCTCCATCAAGACTCTAGATTAAGAATTATCCATAGAGATATGAAGGCTAGCAATATCTTGCTGGACAATGATATGAACCCAAAGATTTCAGACTTCGGCCTGGCTAAAACATTTGGGGGTGATCAAAGTGAAGACAGTACAAATAGAGTGGTTGGAACATATGGTTATATGGCTCCTGAATATGCAGTAGATGGAACTTTCTCAATGAAATCTGATGTATTTAGCTTTGGAGTTGTACTGCTAGAGATATTGAGCAGGAAGAAGAACAGGGGATTTTGTCATCCAGATCACCATCTTAACCTTCTTGGACATGCATGGACACTATGGACTCAAAATAATCCTCTGGAACTGGTTGATGTGACGTTATGTGATTCTTGCAACACATCCGGAGTGTTGAGATGTCTTCATGTGGGCCTGTTATGTGCGCAACAAGCACCTGAAGATAGACCAAGCATGTCATCTGTGGTTCTAATGTTGGGAAATGAAATTAAGTTGCCTACGCCAAAAGAGCCTGGTTTTTACACTGGACGGACCGTTCCAGTTCCTGAATCAACATCAACGACAAGTGACTTGTGCTCAGCAAATAGTTTCAGCACAATGTTCTTAGAATCTCGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

734

Amino Acids

81.95

Weight (kDa)

7.07

Isoelectric Point (pI)

38.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 67 - 160 2.7e-33 D-mannose binding lectin
S_locus_glycop PF00954 172 - 279 8.2e-24 S-locus glycoprotein domain
PAN_2 PF08276 300 - 366 1.7e-21 PAN-like domain
Pkinase PF00069 438 - 616 1.1e-37 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 439 - 684 4.7e-41 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000207)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G27300
fragaria_vesca FvH4_3g15930 FvH4_3g15980 FvH4_3g15980 FvH4_3g15980 FvH4_3g15980 FvH4_3g21350 FvH4_3g21400 FvH4_3g21400 FvH4_6g12332
malus_domestica MD02G1167100.v1.1 MD02G1167200.v1.1 MD03G1185600.v1.1 MD05G1213900.v1.1 MD05G1214100.v1.1 MD05G1214200.v1.1 MD05G1214700.v1.1 MD05G1216300.v1.1 MD05G1216800.v1.1 MD05G1217300.v1.1 MD05G1218000.v1.1 MD11G1231100.v1.1 MD11G1231200.v1.1 MD11G1231400.v1.1 MD11G1231500.v1.1 MD11G1232400.v1.1 MD11G1232500.v1.1
prunus_persica Prupe.4G142300_v2.0.a1 Prupe.4G142400_v2.0.a1 Prupe.4G142500_v2.0.a1 Prupe.4G142600_v2.0.a1 Prupe.4G142800_v2.0.a1 Prupe.4G195000_v2.0.a1 Prupe.4G195000_v2.0.a1 Prupe.4G195000_v2.0.a1 Prupe.4G195100_v2.0.a1 Prupe.4G195300_v2.0.a1 Prupe.4G195300_v2.0.a1 Prupe.4G195300_v2.0.a1 Prupe.4G195400_v2.0.a1 Prupe.4G195400_v2.0.a1 Prupe.4G195500_v2.0.a1 Prupe.4G195700_v2.0.a1 Prupe.4G195900_v2.0.a1
pyrus_communis pycom03g16540 pycom05g19920 pycom11g20460 pycom11g20480 pycom11g20490 pycom11g20500 pycom11g20530
rosa_chinensis RchiOBHm_Chr2g0139311 RchiOBHm_Chr2g0139351 RchiOBHm_Chr5g0026801 RchiOBHm_Chr5g0026851 RchiOBHm_Chr5g0026891 RchiOBHm_Chr5g0026911 RchiOBHm_Chr5g0036571 RchiOBHm_Chr5g0036591 RchiOBHm_Chr5g0036721 RchiOBHm_Chr5g0036741 RchiOBHm_Chr7g0222981
rosa_laevigata RLG00000002006 RLG00000019807 RLG00000032964 RLG00000032968 RLG00000032969 RLG00000033706 RLG00000033708 RLG00000033712 RLG00000033716
rosa_multiflora Rmu_co8256651.1_g000001 Rmu_co8279377.1_g000001 Rmu_co8429919.1_g000001 Rmu_co8490103.1_g000001 Rmu_sc0000774.1_g000002 Rmu_sc0002525.1_g000012 Rmu_sc0004223.1_g000008 Rmu_sc0004390.1_g000002 Rmu_sc0006059.1_g000014 Rmu_sc0006059.1_g000024 Rmu_sc0006514.1_g000020 Rmu_sc0014333.1_g000002 Rmu_ssc0000172.1_g000018 Rmu_ssc0000172.1_g000029
rosa_roxburghii Rroxscaffold_1G00044230 Rroxscaffold_1G00044260 Rroxscaffold_1G00044320 Rroxscaffold_1G00044340 Rroxscaffold_1G00052560 Rroxscaffold_1G00052570 Rroxscaffold_1G00052660 Rroxscaffold_1G00052720 Rroxscaffold_2G00105450 Rroxscaffold_2G00105460
rosa_rugosa Rorug02G0356900 Rorug02G0357000 Rorug02G0357100 Rorug02G0357200 Rorug02G0357300 Rorug02G0357400 Rorug02G0357500 Rorug02G0357600 Rorug05G0095400 Rorug05G0095500 Rorug05G0095500 Rorug05G0095600 Rorug05G0095700 Rorug05G0095800 Rorug05G0157700 Rorug05G0157800
rosa_samantha Rh2AG406800 Rh2AG406900 Rh2BG417300 Rh2CG392900 Rh2DG426200 Rh5AG189300 Rh5AG249400 Rh5AG250100 Rh5BG186300 Rh5BG186900 Rh5BG187000 Rh5BG249500 Rh5BG250600 Rh5BG250900 Rh5BG251500 Rh5BG251800 Rh5CG205500 Rh5CG206300 Rh5CG206700 Rh5CG283000 Rh5CG283300 Rh5CG283500 Rh5DG188500 Rh5DG188700 Rh5DG258700 Rh5DG258900 Rh5DG259600 Rh5DG259900 Rh7CG375800 Rh7DG352500
rosa_wichuraiana Rw2G032960 Rw2G032970 Rw2G033220 Rw2G033230 Rw5G017130 Rw5G017230 Rw5G022940 Rw5G022960 Rw5G022980 Rw7G030420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 104
AasI GACNNNNNNGTC 1 cut(s) 862
Acc16I TGCGCA 1 cut(s) 2010
Acc36I ACCTGC 1 cut(s) 104
AccII CGCG 1 cut(s) 706
AccIII TCCGGA 1 cut(s) 1970
AciI CCGC 1 cut(s) 575
AclWI GGATC 1 cut(s) 1151
AcsI RAATTY 8 cut(s) 273, 475, 1036, 1103, 1203, 1345, 1455, 1469
AcuI CTGAAG 2 cut(s) 1385, 2043
AfaI GTAC 8 cut(s) 182, 620, 808, 828, 926, 1024, 1723, 1821
AgeI ACCGGT 1 cut(s) 633
AgsI TTSAA 4 cut(s) 539, 817, 1372, 1442
AjnI CCWGG 4 cut(s) 959, 993, 1683, 2095
AjuI GAANNNNNNNTTGG 2 cut(s) 450, 482
AloI GAACNNNNNNTCC 2 cut(s) 2103, 2135
Alw21I GWGCWC 1 cut(s) 2162
Alw26I GTCTC 3 cut(s) 191, 352, 878
AlwI GGATC 1 cut(s) 1151
AlwNI CAGNNNCTG 1 cut(s) 2129
Ama87I CYCGRG 1 cut(s) 1569
Aor13HI TCCGGA 1 cut(s) 1970
AoxI GGCC 3 cut(s) 1535, 1681, 1996
ApeKI GCWGC 3 cut(s) 900, 1065, 1142
ApoI RAATTY 8 cut(s) 273, 475, 1036, 1103, 1203, 1345, 1455, 1469
AsiGI ACCGGT 1 cut(s) 633
Asp700I GAANNNNTTC 1 cut(s) 1015
AspLEI GCGC 1 cut(s) 2011
AspS9I GGNCC 4 cut(s) 978, 1536, 1996, 2115
AsuC2I CCSGG 1 cut(s) 147
AsuHPI GGTGA 4 cut(s) 1099, 1304, 1715, 1865
AsuNHI GCTAGC 2 cut(s) 1195, 1631
AvaI CYCGRG 1 cut(s) 1569
AvaII GGWCC 2 cut(s) 978, 2115
BbsI GAAGAC 2 cut(s) 1722, 1979
Bbv12I GWGCWC 1 cut(s) 2162
BbvI GCAGC 3 cut(s) 912, 1052, 1129
BccI CCATC 6 cut(s) 86, 380, 905, 1596, 1769, 1884
BceAI ACGGC 2 cut(s) 547, 1151
BciT130I CCWGG 4 cut(s) 961, 995, 1685, 2097
BclI TGATCA 3 cut(s) 265, 1507, 1705
BcnI CCSGG 1 cut(s) 147
BcoDI GTCTC 3 cut(s) 191, 352, 878
BfaI CTAG 8 cut(s) 513, 777, 1154, 1196, 1599, 1632, 1826, 2200
BfmI CTRYAG 2 cut(s) 333, 906
BfuAI ACCTGC 1 cut(s) 104
BisI GCNGC 3 cut(s) 901, 1066, 1143
BlpI GCTNAGC 1 cut(s) 2161
BlsI GCNGC 3 cut(s) 902, 1067, 1144
Bme1390I CCNGG 5 cut(s) 147, 961, 995, 1685, 2097
Bme18I GGWCC 2 cut(s) 978, 2115
BmeT110I CYCGRG 1 cut(s) 1569
BmgT120I GGNCC 4 cut(s) 978, 1536, 1996, 2115
BmiI GGNNCC 5 cut(s) 717, 980, 1085, 1253, 1758
BmrFI CCNGG 5 cut(s) 147, 961, 995, 1685, 2097
BmrI ACTGGG 1 cut(s) 140
BmtI GCTAGC 2 cut(s) 1199, 1635
BmuI ACTGGG 1 cut(s) 140
BpiI GAAGAC 2 cut(s) 1722, 1979
BpmI CTGGAG 1 cut(s) 80
Bpu1102I GCTNAGC 1 cut(s) 2161
BpuEI CTTGAG 3 cut(s) 717, 968, 1104
BpuMI CCSGG 1 cut(s) 147
BsaBI GATNNNNATC 1 cut(s) 1875
BsaJI CCNNGG 1 cut(s) 994
BsaWI WCCGGW 2 cut(s) 633, 1970
Bse118I RCCGGY 1 cut(s) 633
Bse1I ACTGG 8 cut(s) 135, 344, 550, 569, 1538, 1938, 2113, 2123
Bse3DI GCAATG 1 cut(s) 672
Bse8I GATNNNNATC 1 cut(s) 1875
BseAI TCCGGA 1 cut(s) 1970
BseBI CCWGG 4 cut(s) 961, 995, 1685, 2097
BseDI CCNNGG 1 cut(s) 994
BseGI GGATG 5 cut(s) 314, 607, 878, 1864, 1967
BseJI GATNNNNATC 1 cut(s) 1875
BseMI GCAATG 1 cut(s) 672
BseMII CTCAG 4 cut(s) 186, 1161, 1294, 2175
BseNI ACTGG 8 cut(s) 135, 344, 550, 569, 1538, 1938, 2113, 2123
BseRI GAGGAG 2 cut(s) 617, 1326
BseXI GCAGC 3 cut(s) 912, 1052, 1129
BseYI CCCAGC 1 cut(s) 518
Bsh1236I CGCG 1 cut(s) 706
BshFI GGCC 3 cut(s) 1537, 1683, 1998
BshTI ACCGGT 1 cut(s) 633
BsiHKAI GWGCWC 1 cut(s) 2162
BsiHKCI CYCGRG 1 cut(s) 1569
BsiSI CCGG 3 cut(s) 147, 634, 1971
BslFI GGGAC 2 cut(s) 129, 535
BsmAI GTCTC 3 cut(s) 191, 352, 878
BsmFI GGGAC 2 cut(s) 129, 535
BsmI GAATGC 2 cut(s) 1242, 1437
BsnI GGCC 3 cut(s) 1537, 1683, 1998
BsoBI CYCGRG 1 cut(s) 1569
Bsp1286I GDGCHC 1 cut(s) 2162
Bsp13I TCCGGA 1 cut(s) 1970
Bsp1407I TGTACA 1 cut(s) 806
Bsp143I GATC 7 cut(s) 265, 739, 1156, 1209, 1507, 1705, 1870
Bsp1720I GCTNAGC 1 cut(s) 2161
BspACI CCGC 1 cut(s) 575
BspANI GGCC 3 cut(s) 1537, 1683, 1998
BspCNI CTCAG 4 cut(s) 187, 1160, 1293, 2174
BspEI TCCGGA 1 cut(s) 1970
BspFNI CGCG 1 cut(s) 706
BspLI GGNNCC 5 cut(s) 717, 980, 1085, 1253, 1758
BspMAI CTGCAG 1 cut(s) 337
BspMI ACCTGC 1 cut(s) 104
BspOI GCTAGC 2 cut(s) 1199, 1635
BspPI GGATC 1 cut(s) 1151
BsrDI GCAATG 1 cut(s) 672
BsrFI RCCGGY 1 cut(s) 633
BsrGI TGTACA 1 cut(s) 806
BsrI ACTGG 8 cut(s) 135, 344, 550, 569, 1538, 1938, 2113, 2123
BssAI RCCGGY 1 cut(s) 633
BssECI CCNNGG 1 cut(s) 994
BssMI GATC 7 cut(s) 265, 739, 1156, 1209, 1507, 1705, 1870
Bst2UI CCWGG 4 cut(s) 961, 995, 1685, 2097
Bst4CI ACNGT 7 cut(s) 202, 307, 681, 929, 1016, 1721, 2119
Bst6I CTCTTC 1 cut(s) 1265
BstAUI TGTACA 1 cut(s) 806
BstC8I GCNNGC 6 cut(s) 452, 898, 1070, 1140, 1197, 1633
BstDEI CTNAG 8 cut(s) 41, 195, 501, 597, 1147, 1280, 2161, 2188
BstF5I GGATG 5 cut(s) 314, 607, 878, 1864, 1967
BstFNI CGCG 1 cut(s) 706
BstHHI GCGC 1 cut(s) 2011
BstKTI GATC 7 cut(s) 268, 742, 1159, 1212, 1510, 1708, 1873
BstMAI GTCTC 3 cut(s) 191, 352, 878
BstMBI GATC 7 cut(s) 265, 739, 1156, 1209, 1507, 1705, 1870
BstMWI GCNNNNNNNGC 5 cut(s) 300, 1182, 1534, 2015, 2092
BstNI CCWGG 4 cut(s) 961, 995, 1685, 2097
BstNSI RCATGY 4 cut(s) 813, 1545, 1898, 2041
BstSCI CCNGG 5 cut(s) 145, 959, 993, 1683, 2095
BstSFI CTRYAG 2 cut(s) 333, 906
BstUI CGCG 1 cut(s) 706
BstV1I GCAGC 3 cut(s) 912, 1052, 1129
BstV2I GAAGAC 2 cut(s) 1722, 1979
BstX2I RGATCY 1 cut(s) 1156
BstYI RGATCY 1 cut(s) 1156
BsuRI GGCC 3 cut(s) 1537, 1683, 1998
BtsCI GGATG 5 cut(s) 314, 607, 878, 1864, 1967
BtsIMutI CAGTG 5 cut(s) 261, 303, 686, 749, 2106
BveI ACCTGC 1 cut(s) 104
Cac8I GCNNGC 6 cut(s) 452, 898, 1070, 1140, 1197, 1633
CaiI CAGNNNCTG 1 cut(s) 2129
CfoI GCGC 1 cut(s) 2011
Cfr10I RCCGGY 1 cut(s) 633
Cfr13I GGNCC 4 cut(s) 978, 1536, 1996, 2115
CpoI CGGWCCG 1 cut(s) 2115
Csp6I GTAC 8 cut(s) 181, 619, 807, 827, 925, 1023, 1722, 1820
CspAI ACCGGT 1 cut(s) 633
CspCI CAANNNNNGTGG 2 cut(s) 1713, 1748
CspI CGGWCCG 1 cut(s) 2115
CviAII CATG 9 cut(s) 415, 525, 810, 1474, 1542, 1895, 1899, 1991, 2038
CviQI GTAC 8 cut(s) 181, 619, 807, 827, 925, 1023, 1722, 1820
DdeI CTNAG 8 cut(s) 41, 195, 501, 597, 1147, 1280, 2161, 2188
DpnI GATC 7 cut(s) 267, 741, 1158, 1211, 1509, 1707, 1872
DpnII GATC 7 cut(s) 265, 739, 1156, 1209, 1507, 1705, 1870
DraI TTTAAA 2 cut(s) 255, 937
DrdI GACNNNNNNGTC 1 cut(s) 862
DseDI GACNNNNNNGTC 1 cut(s) 862
Eam1104I CTCTTC 1 cut(s) 1265
EarI CTCTTC 1 cut(s) 1265
Eco32I GATATC 1 cut(s) 551
Eco47I GGWCC 2 cut(s) 978, 2115
Eco57I CTGAAG 2 cut(s) 1385, 2043
Eco88I CYCGRG 1 cut(s) 1569
EcoO109I RGGNCCY 1 cut(s) 978
EcoRI GAATTC 3 cut(s) 1103, 1203, 1469
EcoRII CCWGG 4 cut(s) 959, 993, 1683, 2095
EcoRV GATATC 1 cut(s) 551
EcoT22I ATGCAT 1 cut(s) 1900
FaeI CATG 9 cut(s) 418, 528, 813, 1477, 1545, 1898, 1902, 1994, 2041
FaqI GGGAC 2 cut(s) 129, 535
FatI CATG 9 cut(s) 414, 524, 809, 1473, 1541, 1894, 1898, 1990, 2037
FauNDI CATATG 3 cut(s) 667, 1030, 1744
FbaI TGATCA 3 cut(s) 265, 1507, 1705
Fnu4HI GCNGC 3 cut(s) 901, 1066, 1143
FokI GGATG 5 cut(s) 301, 614, 885, 1851, 1954
Fsp4HI GCNGC 3 cut(s) 901, 1066, 1143
FspBI CTAG 8 cut(s) 513, 777, 1154, 1196, 1599, 1632, 1826, 2200
FspI TGCGCA 1 cut(s) 2010
GlaI GCGC 1 cut(s) 2010
GluI GCNGC 3 cut(s) 901, 1066, 1143
GsaI CCCAGC 1 cut(s) 522
GsuI CTGGAG 1 cut(s) 80
HaeIII GGCC 3 cut(s) 1537, 1683, 1998
HapII CCGG 3 cut(s) 147, 634, 1971
HhaI GCGC 1 cut(s) 2011
Hin1II CATG 9 cut(s) 418, 528, 813, 1477, 1545, 1898, 1902, 1994, 2041
Hin6I GCGC 1 cut(s) 2009
HinP1I GCGC 1 cut(s) 2009
HincII GTYRAC 1 cut(s) 594
HindII GTYRAC 1 cut(s) 594
HindIII AAGCTT 3 cut(s) 464, 1421, 1486
HpaII CCGG 3 cut(s) 147, 634, 1971
HphI GGTGA 4 cut(s) 1099, 1304, 1715, 1865
Hpy166II GTNNAC 4 cut(s) 183, 594, 621, 1023
Hpy188I TCNGA 7 cut(s) 840, 1114, 1150, 1209, 1283, 1675, 1798
Hpy8I GTNNAC 4 cut(s) 183, 594, 621, 1023
HpyAV CCTTC 6 cut(s) 46, 293, 487, 1046, 1621, 1895
HpyCH4III ACNGT 7 cut(s) 202, 307, 681, 929, 1016, 1721, 2119
HpyCH4IV ACGT 1 cut(s) 1946
HpyF10VI GCNNNNNNNGC 5 cut(s) 300, 1182, 1534, 2015, 2092
HpyF3I CTNAG 8 cut(s) 41, 195, 501, 597, 1147, 1280, 2161, 2188
HpySE526I ACGT 1 cut(s) 1946
Hsp92II CATG 9 cut(s) 418, 528, 813, 1477, 1545, 1898, 1902, 1994, 2041
HspAI GCGC 1 cut(s) 2009
Kpn2I TCCGGA 1 cut(s) 1970
Ksp22I TGATCA 3 cut(s) 265, 1507, 1705
Kzo9I GATC 7 cut(s) 265, 739, 1156, 1209, 1507, 1705, 1870
LmnI GCTCC 3 cut(s) 497, 802, 1762
Lsp1109I GCAGC 3 cut(s) 912, 1052, 1129
MaeI CTAG 8 cut(s) 513, 777, 1154, 1196, 1599, 1632, 1826, 2200
MaeII ACGT 1 cut(s) 1946
MaeIII GTNAC 5 cut(s) 259, 791, 929, 1942, 2150
MalI GATC 7 cut(s) 267, 741, 1158, 1211, 1509, 1707, 1872
MboI GATC 7 cut(s) 265, 739, 1156, 1209, 1507, 1705, 1870
MflI RGATCY 1 cut(s) 1156
MhlI GDGCHC 1 cut(s) 2162
MlyI GAGTC 4 cut(s) 71, 880, 1589, 1906
MmeI TCCRAC 2 cut(s) 452, 1717
Mph1103I ATGCAT 1 cut(s) 1900
MroI TCCGGA 1 cut(s) 1970
MroXI GAANNNNTTC 1 cut(s) 1015
MspI CCGG 3 cut(s) 147, 634, 1971
MspR9I CCNGG 5 cut(s) 147, 961, 995, 1685, 2097
Mva1269I GAATGC 2 cut(s) 1242, 1437
MvaI CCWGG 4 cut(s) 961, 995, 1685, 2097
MvnI CGCG 1 cut(s) 706
MwoI GCNNNNNNNGC 5 cut(s) 300, 1182, 1534, 2015, 2092
NciI CCSGG 1 cut(s) 147
NdeI CATATG 3 cut(s) 667, 1030, 1744
NdeII GATC 7 cut(s) 265, 739, 1156, 1209, 1507, 1705, 1870
NheI GCTAGC 2 cut(s) 1195, 1631
NlaIII CATG 9 cut(s) 418, 528, 813, 1477, 1545, 1898, 1902, 1994, 2041
NlaIV GGNNCC 5 cut(s) 717, 980, 1085, 1253, 1758
NmuCI GTSAC 3 cut(s) 259, 1942, 2150
NsbI TGCGCA 1 cut(s) 2010
NsiI ATGCAT 1 cut(s) 1900
NspI RCATGY 4 cut(s) 813, 1545, 1898, 2041
PaeR7I CTCGAG 1 cut(s) 1569
PaqCI CACCTGC 1 cut(s) 104
PctI GAATGC 2 cut(s) 1242, 1437
PdmI GAANNNNTTC 1 cut(s) 1015
PinAI ACCGGT 1 cut(s) 633
PkrI GCNGC 3 cut(s) 902, 1067, 1144
PleI GAGTC 4 cut(s) 70, 880, 1589, 1906
PpsI GAGTC 4 cut(s) 70, 880, 1589, 1906
PpuMI RGGWCCY 1 cut(s) 978
Psp5II RGGWCCY 1 cut(s) 978
Psp6I CCWGG 4 cut(s) 959, 993, 1683, 2095
PspFI CCCAGC 1 cut(s) 518
PspGI CCWGG 4 cut(s) 959, 993, 1683, 2095
PspN4I GGNNCC 5 cut(s) 717, 980, 1085, 1253, 1758
PspPI GGNCC 4 cut(s) 978, 1536, 1996, 2115
PspPPI RGGWCCY 1 cut(s) 978
PspXI VCTCGAGB 1 cut(s) 1569
PstI CTGCAG 1 cut(s) 337
PstNI CAGNNNCTG 1 cut(s) 2129
PsuI RGATCY 1 cut(s) 1156
RsaI GTAC 8 cut(s) 182, 620, 808, 828, 926, 1024, 1723, 1821
RsaNI GTAC 8 cut(s) 181, 619, 807, 827, 925, 1023, 1722, 1820
Rsr2I CGGWCCG 1 cut(s) 2115
RsrII CGGWCCG 1 cut(s) 2115
SatI GCNGC 3 cut(s) 901, 1066, 1143
Sau3AI GATC 7 cut(s) 265, 739, 1156, 1209, 1507, 1705, 1870
Sau96I GGNCC 4 cut(s) 978, 1536, 1996, 2115
SchI GAGTC 4 cut(s) 71, 880, 1589, 1906
ScrFI CCNGG 5 cut(s) 147, 961, 995, 1685, 2097
SduI GDGCHC 1 cut(s) 2162
SfcI CTRYAG 2 cut(s) 333, 906
Sfr274I CTCGAG 1 cut(s) 1569
SinI GGWCC 2 cut(s) 978, 2115
SlaI CTCGAG 1 cut(s) 1569
SmlI CTYRAG 4 cut(s) 732, 983, 1119, 1569
SmoI CTYRAG 4 cut(s) 732, 983, 1119, 1569
SsiI CCGC 1 cut(s) 575
SspMI CTAG 8 cut(s) 513, 777, 1154, 1196, 1599, 1632, 1826, 2200
StyD4I CCNGG 5 cut(s) 145, 959, 993, 1683, 2095
TaaI ACNGT 7 cut(s) 202, 307, 681, 929, 1016, 1721, 2119
TaiI ACGT 1 cut(s) 1949
TaqI TCGA 3 cut(s) 854, 1530, 1570
TatI WGTACW 6 cut(s) 618, 806, 924, 1022, 1721, 1819
TscAI CASTG 5 cut(s) 268, 310, 686, 749, 2113
TseFI GTSAC 3 cut(s) 259, 1942, 2150
TseI GCWGC 3 cut(s) 900, 1065, 1142
Tsp45I GTSAC 3 cut(s) 259, 1942, 2150
TspGWI ACGGA 2 cut(s) 1282, 2128
TspRI CASTG 5 cut(s) 268, 310, 686, 749, 2113
VpaK11BI GGWCC 2 cut(s) 978, 2115
XapI RAATTY 8 cut(s) 273, 475, 1036, 1103, 1203, 1345, 1455, 1469
XbaI TCTAGA 1 cut(s) 1598
XceI RCATGY 4 cut(s) 813, 1545, 1898, 2041
XhoI CTCGAG 1 cut(s) 1569
XmnI GAANNNNTTC 1 cut(s) 1015
XspI CTAG 8 cut(s) 513, 777, 1154, 1196, 1599, 1632, 1826, 2200
Zsp2I ATGCAT 1 cut(s) 1900
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.