RchiOBHm_Chr5g0026851

divergent subfamily of APPLE domains

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000207
Physical Location & Seq
Reverse (-)
20845042 .. 20848424
3383 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ30643

Sequence Viewer

Length: 2508 bp
ATGGATTACTTTCTTTCAGTAAGAAAATTACATTCTGATCAGAGATCAATGCAAGCCTTTAGAACCCTTTTTGTGTGCTACTTCTTTTTCTCCTCCGTAAAAACCCCATTTACAACTGCACTAGATGCTATCACTCCAAGTCAATATATTAGAGATGGTGAAACTCTAGTTTCTGCTGATGGAAACTTTGAACTGGGATTCTTTGGTAAGTCGAAAGGCCAATACTTGGGAATATGGTACACTTTCTCTACTGACATAGTTGTGTGGGTAGCCAACAGAGAAACACCGGTTAATGATTCTTCAGGAGTCTTAAAGCTCACTGATCAGGGAGTTTTAGTCCTTCTCAATAGCTCAAATGGCACTGTATGGTCAACCAATTCATCAAGAACTGCAGGAAATCCAGTCTTGCAACTCTTGGATTCGGGAAATCTTGTTGTGAAAGATGGAAATGATACTAGCCCTGATTACTTTCTTTGGCAGAGTTTTGATTATCCTTGTGACACACAACTACCAGGAATGAAACTTGGCCGGAACTTAGTTAATGGTTTAGACAGGTATCTCTCGTCCTGGAGGAGCACAGATGATCCTGCTCAAGGAGAGTTTTCACTACGGATGGATCCTCGTGGTTTACCTCAATTTTTTGAAGTTCAGGGAGCTAAGATACTGACTAGAGCAGGATCATGGAACGGCCTTCAATTGACTGGATATCAAAGGAGGCCAAATCCAATATCTGAGTTTTTATTTGTGTTGAATGAGACAGAAATCTATTATGAGTACACACTCCTCAACCGGTCTACGTTCTCTAGATATGTAATGAATCCATATGGCACTACACAGTGGTTAACATGGATAGACTACACAAATAGTTGGGAGCCTTTCTTTGCAAGCCAAGCAGATCAGTGTGAAATTTACGCTTTTTGTGGGGCTAATGCTAATTGTAATGTCAATGAAGCCCCCATGTGTGCATGCTTGAAAGGATTTGTACCTAAATCTCCACAAAAGTGGAACTCTTCACATTGGTCTGATGGATGTGTTCGAAGGACTCCATTAGTTTGCAGTGATAGAGATGACTTCTTAATATATAGTAGATTTAAATTGCCAGACACATCTTCTTCCTGGTATGACAAGAGCATGAGCCTCAAGGAATGCAAGGAATTGTGTTTGAGAAACTGCTCGTGTACGGCTTATGCAAATTTAGATGTCAGGGAAGGAGGAAGTGGCTGCTTGCTTTGGTTTGGAAACCTCACTGACATAAGAGAATTTACCTCTGATTCTCAAGACCTCTATATACGAATAGCTTCTTCAGACACAGATGCTATTGGGAAGAAGAGCAAGTCCAACAAGAGTAGGCAAGCTGGAATTGTAATCAGCTCTGCTCTTCTTGTAGTGGGAATGCTAATACTTGGATTCTTCTTGTATAAACGGAACAAGAAACTCAGAAATCAAGATGTTAGAAGATTGCTGGATTGCCAAATGGATGACTGTGAAGAAGGAAGGGAAGACATGGAGTTACCGCTATTTGACTTTACCACTGTAGCTGATGCCACTGAAAACTTTTCAAGCAACAACAAACTGGGACAAGGCGGTTTTGGACCTGTGTACAAGGGTACATTGATAGGAGGGAAAGAAATAGCTGTAAAGAGGCGATCCAAGGAATCTGGTCAAGGAATGAGGGAGTTCAAAAATGAAGTTCTACTGATAGCCAAACTTCAGCACCGTAATCTTGTAAAGCTTCTGGGCTGTTGCATTCAAAATGATGAAAAAATGTTAATATATGAATACATGTCCAACAGGAGCTTGGACTTCTTTATATTTGATCAGGAAAGACAGAAATCGCTCGACTGGCCTTCCTGCTACAACATTATTGACGGAACTGCACGAGGGCTTCTCTATCTTCACCAAGACTCGAGATTAAGAATTATTCACAGAGATCTGAAACCTAGTAATATTTTGCTAGATGAAAACATGAACCCAAGGATTTCAGATTTTGGCCTGGCTAAAACATTTAGCTTTGATCAAAGTCAAGCCAATACGAATAAAGTGGCTGGAACATATGGTTATATGGCGCCTGAATATGCAGTAGATGGAATTTTCTCGACGAAATCAGATGTATTTAGCTTTGGAGTTGTGCTGATTGAGTTATTGAGTAGGGAGAAGAACAGGGGATTTTGTCATCCAGATCACGATTTTAATCTTCTTGGACATGCATGGACACTATGGACTCAAAATGTACCACTGGAACTCATCGAGAAGACATTATGTGATCCGGATACCATATCCGAAGTTTTAAGGTGTCTTCATGTGGGTCTGTTATGTGTGCAGCAAGTACCTGAAGATAGACCAAGCATGTCATCTGTGGTACTAATGTTGGGCAGTCATGATGTTGTGCTGCCTTTGCCAAAGCAGCCTGGTTTTTACACTGAACGGACTCTTCCCGAATCATCATCAAGGTCACGTGACCTGTGTTCACCCAGTAACTTCAGCACTACATTGTTAGAGGCTCGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

835

Amino Acids

94.6

Weight (kDa)

5.77

Isoelectric Point (pI)

44.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 87 - 191 1.7e-37 D-mannose binding lectin
S_locus_glycop PF00954 224 - 331 5e-23 S-locus glycoprotein domain
PAN_2 PF08276 352 - 418 1.7e-21 PAN-like domain
Pkinase PF00069 520 - 786 4.8e-45 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 522 - 787 2.1e-45 Protein tyrosine and serine/threonine kinase
DUF3403 PF11883 793 - 835 4.2e-07 Domain of unknown function (DUF3403)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000207)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G27300
fragaria_vesca FvH4_3g15930 FvH4_3g15980 FvH4_3g15980 FvH4_3g15980 FvH4_3g15980 FvH4_3g21350 FvH4_3g21400 FvH4_3g21400 FvH4_6g12332
malus_domestica MD02G1167100.v1.1 MD02G1167200.v1.1 MD03G1185600.v1.1 MD05G1213900.v1.1 MD05G1214100.v1.1 MD05G1214200.v1.1 MD05G1214700.v1.1 MD05G1216300.v1.1 MD05G1216800.v1.1 MD05G1217300.v1.1 MD05G1218000.v1.1 MD11G1231100.v1.1 MD11G1231200.v1.1 MD11G1231400.v1.1 MD11G1231500.v1.1 MD11G1232400.v1.1 MD11G1232500.v1.1
prunus_persica Prupe.4G142300_v2.0.a1 Prupe.4G142400_v2.0.a1 Prupe.4G142500_v2.0.a1 Prupe.4G142600_v2.0.a1 Prupe.4G142800_v2.0.a1 Prupe.4G195000_v2.0.a1 Prupe.4G195000_v2.0.a1 Prupe.4G195000_v2.0.a1 Prupe.4G195100_v2.0.a1 Prupe.4G195300_v2.0.a1 Prupe.4G195300_v2.0.a1 Prupe.4G195300_v2.0.a1 Prupe.4G195400_v2.0.a1 Prupe.4G195400_v2.0.a1 Prupe.4G195500_v2.0.a1 Prupe.4G195700_v2.0.a1 Prupe.4G195900_v2.0.a1
pyrus_communis pycom03g16540 pycom05g19920 pycom11g20460 pycom11g20480 pycom11g20490 pycom11g20500 pycom11g20530
rosa_chinensis RchiOBHm_Chr2g0139311 RchiOBHm_Chr2g0139351 RchiOBHm_Chr5g0026801 RchiOBHm_Chr5g0026851 RchiOBHm_Chr5g0026891 RchiOBHm_Chr5g0026911 RchiOBHm_Chr5g0036571 RchiOBHm_Chr5g0036591 RchiOBHm_Chr5g0036721 RchiOBHm_Chr5g0036741 RchiOBHm_Chr7g0222981
rosa_laevigata RLG00000002006 RLG00000019807 RLG00000032964 RLG00000032968 RLG00000032969 RLG00000033706 RLG00000033708 RLG00000033712 RLG00000033716
rosa_multiflora Rmu_co8256651.1_g000001 Rmu_co8279377.1_g000001 Rmu_co8429919.1_g000001 Rmu_co8490103.1_g000001 Rmu_sc0000774.1_g000002 Rmu_sc0002525.1_g000012 Rmu_sc0004223.1_g000008 Rmu_sc0004390.1_g000002 Rmu_sc0006059.1_g000014 Rmu_sc0006059.1_g000024 Rmu_sc0006514.1_g000020 Rmu_sc0014333.1_g000002 Rmu_ssc0000172.1_g000018 Rmu_ssc0000172.1_g000029
rosa_roxburghii Rroxscaffold_1G00044230 Rroxscaffold_1G00044260 Rroxscaffold_1G00044320 Rroxscaffold_1G00044340 Rroxscaffold_1G00052560 Rroxscaffold_1G00052570 Rroxscaffold_1G00052660 Rroxscaffold_1G00052720 Rroxscaffold_2G00105450 Rroxscaffold_2G00105460
rosa_rugosa Rorug02G0356900 Rorug02G0357000 Rorug02G0357100 Rorug02G0357200 Rorug02G0357300 Rorug02G0357400 Rorug02G0357500 Rorug02G0357600 Rorug05G0095400 Rorug05G0095500 Rorug05G0095500 Rorug05G0095600 Rorug05G0095700 Rorug05G0095800 Rorug05G0157700 Rorug05G0157800
rosa_samantha Rh2AG406800 Rh2AG406900 Rh2BG417300 Rh2CG392900 Rh2DG426200 Rh5AG189300 Rh5AG249400 Rh5AG250100 Rh5BG186300 Rh5BG186900 Rh5BG187000 Rh5BG249500 Rh5BG250600 Rh5BG250900 Rh5BG251500 Rh5BG251800 Rh5CG205500 Rh5CG206300 Rh5CG206700 Rh5CG283000 Rh5CG283300 Rh5CG283500 Rh5DG188500 Rh5DG188700 Rh5DG258700 Rh5DG258900 Rh5DG259600 Rh5DG259900 Rh7CG375800 Rh7DG352500
rosa_wichuraiana Rw2G032960 Rw2G032970 Rw2G033220 Rw2G033230 Rw5G017130 Rw5G017230 Rw5G022940 Rw5G022960 Rw5G022980 Rw7G030420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 2065
AccB7I CCANNNNNTGG 1 cut(s) 226
AccI GTMKAC 1 cut(s) 794
AccIII TCCGGA 1 cut(s) 2266
AciI CCGC 2 cut(s) 1514, 1584
AclWI GGATC 6 cut(s) 578, 611, 624, 685, 1641, 2258
AcoI YGGCCR 1 cut(s) 526
AcsI RAATTY 4 cut(s) 906, 1192, 1259, 2088
AcuI CTGAAG 5 cut(s) 285, 1287, 1694, 2352, 2464
AcvI CACGTG 1 cut(s) 2456
AcyI GRCGYC 1 cut(s) 2066
AfaI GTAC 9 cut(s) 239, 776, 984, 1180, 1601, 1609, 2232, 2328, 2361
AfiI CCNNNNNNNGG 2 cut(s) 226, 593
AflIII ACRYGT 1 cut(s) 1782
AgeI ACCGGT 2 cut(s) 286, 789
AgsI TTSAA 8 cut(s) 191, 644, 695, 751, 973, 1560, 1681, 1751
AjnI CCWGG 5 cut(s) 511, 566, 1115, 1992, 2407
AleI CACNNNNGTG 1 cut(s) 1000
Alw21I GWGCWC 1 cut(s) 578
Alw26I GTCTC 1 cut(s) 749
AlwI GGATC 6 cut(s) 578, 611, 624, 685, 1641, 2258
Ama87I CYCGRG 1 cut(s) 1906
Aor13HI TCCGGA 1 cut(s) 2266
AoxI GGCC 6 cut(s) 217, 526, 688, 716, 1844, 1990
ApeKI GCWGC 4 cut(s) 1221, 2320, 2389, 2404
ApoI RAATTY 4 cut(s) 906, 1192, 1259, 2088
AsiGI ACCGGT 2 cut(s) 286, 789
Asp700I GAANNNNTTC 1 cut(s) 1297
AspLEI GCGC 1 cut(s) 2068
AspS9I GGNCC 1 cut(s) 1592
AsuHPI GGTGA 3 cut(s) 170, 1889, 2460
AsuII TTCGAA 1 cut(s) 1036
AvaI CYCGRG 1 cut(s) 1906
AvaII GGWCC 1 cut(s) 1592
BaeI ACNNNNGTAYC 2 cut(s) 2351, 2384
BamHI GGATCC 1 cut(s) 616
BanI GGYRCC 1 cut(s) 2065
BauI CACGAG 3 cut(s) 621, 1174, 1878
BbrPI CACGTG 1 cut(s) 2456
BbsI GAAGAC 3 cut(s) 1506, 2258, 2288
Bbv12I GWGCWC 1 cut(s) 578
BbvI GCAGC 4 cut(s) 1208, 2332, 2376, 2416
BccI CCATC 6 cut(s) 149, 173, 437, 607, 1019, 2078
BceAI ACGGC 2 cut(s) 703, 1197
BciT130I CCWGG 5 cut(s) 513, 568, 1117, 1994, 2409
BciVI GTATCC 1 cut(s) 2263
BclI TGATCA 4 cut(s) 37, 322, 1816, 2014
BcoDI GTCTC 1 cut(s) 749
BfaI CTAG 7 cut(s) 122, 167, 456, 669, 804, 1941, 1955
BfmI CTRYAG 2 cut(s) 390, 1533
BfoI RGCGCY 1 cut(s) 2069
BfuI GTATCC 1 cut(s) 2263
BglII AGATCT 1 cut(s) 1930
BisI GCNGC 4 cut(s) 1222, 2321, 2390, 2405
BlsI GCNGC 4 cut(s) 1223, 2322, 2391, 2406
Bme1390I CCNGG 5 cut(s) 513, 568, 1117, 1994, 2409
Bme18I GGWCC 1 cut(s) 1592
BmeT110I CYCGRG 1 cut(s) 1906
BmgT120I GGNCC 1 cut(s) 1592
BmiI GGNNCC 3 cut(s) 618, 873, 2067
BmrFI CCNGG 5 cut(s) 513, 568, 1117, 1994, 2409
BmrI ACTGGG 3 cut(s) 203, 1583, 2466
BmsI GCATC 3 cut(s) 115, 1303, 1531
BmuI ACTGGG 3 cut(s) 203, 1583, 2466
BpiI GAAGAC 3 cut(s) 1506, 2258, 2288
BplI GAGNNNNNCTC 2 cut(s) 1872, 1904
BpmI CTGGAG 1 cut(s) 589
Bpu14I TTCGAA 1 cut(s) 1036
BpuEI CTTGAG 3 cut(s) 576, 1124, 1260
BsaAI YACGTR 1 cut(s) 2456
BsaBI GATNNNNATC 1 cut(s) 2190
BsaHI GRCGYC 1 cut(s) 2066
BsaJI CCNNGG 2 cut(s) 1650, 1973
BsaWI WCCGGW 3 cut(s) 286, 789, 2266
BsaXI ACNNNNNCTCC 2 cut(s) 321, 351
Bsc4I CCNNNNNNNGG 2 cut(s) 226, 593
Bse118I RCCGGY 2 cut(s) 286, 789
Bse1I ACTGG 7 cut(s) 198, 401, 706, 1578, 1847, 2241, 2472
Bse8I GATNNNNATC 1 cut(s) 2190
BseAI TCCGGA 1 cut(s) 2266
BseBI CCWGG 5 cut(s) 513, 568, 1117, 1994, 2409
BseDI CCNNGG 2 cut(s) 1650, 1973
BseGI GGATG 4 cut(s) 618, 1034, 1483, 2173
BseJI GATNNNNATC 1 cut(s) 2190
BseLI CCNNNNNNNGG 2 cut(s) 226, 593
BseMII CTCAG 2 cut(s) 723, 1450
BseNI ACTGG 7 cut(s) 198, 401, 706, 1578, 1847, 2241, 2472
BseRI GAGGAG 3 cut(s) 82, 586, 773
BseXI GCAGC 4 cut(s) 1208, 2332, 2376, 2416
BsgI GTGCAG 3 cut(s) 102, 1860, 2339
BshFI GGCC 6 cut(s) 219, 528, 690, 718, 1846, 1992
BshNI GGYRCC 1 cut(s) 2065
BshTI ACCGGT 2 cut(s) 286, 789
BsiHKAI GWGCWC 1 cut(s) 578
BsiHKCI CYCGRG 1 cut(s) 1906
BsiSI CCGG 4 cut(s) 287, 529, 790, 2267
BslFI GGGAC 1 cut(s) 1590
BslI CCNNNNNNNGG 2 cut(s) 226, 593
BsmAI GTCTC 1 cut(s) 749
BsmFI GGGAC 1 cut(s) 1590
BsmI GAATGC 3 cut(s) 1151, 1398, 1746
BsnI GGCC 6 cut(s) 219, 528, 690, 718, 1846, 1992
BsoBI CYCGRG 1 cut(s) 1906
Bsp119I TTCGAA 1 cut(s) 1036
Bsp1286I GDGCHC 1 cut(s) 578
Bsp13I TCCGGA 1 cut(s) 2266
Bsp1407I TGTACA 1 cut(s) 1599
BspACI CCGC 2 cut(s) 1514, 1584
BspANI GGCC 6 cut(s) 219, 528, 690, 718, 1846, 1992
BspCNI CTCAG 2 cut(s) 724, 1449
BspEI TCCGGA 1 cut(s) 2266
BspHI TCATGA 1 cut(s) 2377
BspLI GGNNCC 3 cut(s) 618, 873, 2067
BspMAI CTGCAG 1 cut(s) 394
BspPI GGATC 6 cut(s) 578, 611, 624, 685, 1641, 2258
BspQI GCTCTTC 2 cut(s) 1322, 1383
BspT104I TTCGAA 1 cut(s) 1036
BspT107I GGYRCC 1 cut(s) 2065
BsrFI RCCGGY 2 cut(s) 286, 789
BsrGI TGTACA 1 cut(s) 1599
BsrI ACTGG 7 cut(s) 198, 401, 706, 1578, 1847, 2241, 2472
BssAI RCCGGY 2 cut(s) 286, 789
BssECI CCNNGG 2 cut(s) 1650, 1973
BssNI GRCGYC 1 cut(s) 2066
BssSI CACGAG 3 cut(s) 621, 1174, 1878
BssT1I CCWWGG 2 cut(s) 1650, 1973
Bst2BI CACGAG 3 cut(s) 621, 1174, 1878
Bst2UI CCWGG 5 cut(s) 513, 568, 1117, 1994, 2409
Bst4CI ACNGT 5 cut(s) 364, 837, 1484, 1534, 1718
Bst6I CTCTTC 4 cut(s) 1015, 1322, 1383, 2436
BstACI GRCGYC 1 cut(s) 2066
BstAPI GCANNNNNTGC 1 cut(s) 125
BstAUI TGTACA 1 cut(s) 1599
BstBAI YACGTR 1 cut(s) 2456
BstBI TTCGAA 1 cut(s) 1036
BstC8I GCNNGC 5 cut(s) 54, 886, 967, 1226, 1353
BstDEI CTNAG 4 cut(s) 535, 657, 732, 1436
BstENI CCTNNNNNAGG 1 cut(s) 591
BstF5I GGATG 4 cut(s) 618, 1034, 1483, 2173
BstH2I RGCGCY 1 cut(s) 2069
BstHHI GCGC 1 cut(s) 2068
BstMAI GTCTC 1 cut(s) 749
BstMWI GCNNNNNNNGC 6 cut(s) 125, 357, 890, 1843, 2395, 2404
BstNI CCWGG 5 cut(s) 513, 568, 1117, 1994, 2409
BstNSI RCATGY 4 cut(s) 969, 1786, 2207, 2350
BstSCI CCNGG 5 cut(s) 511, 566, 1115, 1992, 2407
BstSFI CTRYAG 2 cut(s) 390, 1533
BstV1I GCAGC 4 cut(s) 1208, 2332, 2376, 2416
BstV2I GAAGAC 3 cut(s) 1506, 2258, 2288
BstX2I RGATCY 2 cut(s) 616, 1930
BstXI CCANNNNNNTGG 1 cut(s) 1002
BstYI RGATCY 2 cut(s) 616, 1930
BsuI GTATCC 1 cut(s) 2263
BsuRI GGCC 6 cut(s) 219, 528, 690, 718, 1846, 1992
BtsCI GGATG 4 cut(s) 618, 1034, 1483, 2173
BtsI GCAGTG 1 cut(s) 1063
Cac8I GCNNGC 5 cut(s) 54, 886, 967, 1226, 1353
CciI TCATGA 1 cut(s) 2377
CfoI GCGC 1 cut(s) 2068
Cfr10I RCCGGY 2 cut(s) 286, 789
Cfr13I GGNCC 1 cut(s) 1592
Csp6I GTAC 9 cut(s) 238, 775, 983, 1179, 1600, 1608, 2231, 2327, 2360
CspAI ACCGGT 2 cut(s) 286, 789
CviQI GTAC 9 cut(s) 238, 775, 983, 1179, 1600, 1608, 2231, 2327, 2360
DdeI CTNAG 4 cut(s) 535, 657, 732, 1436
DinI GGCGCC 1 cut(s) 2067
DraI TTTAAA 1 cut(s) 1093
EaeI YGGCCR 1 cut(s) 526
Eam1104I CTCTTC 4 cut(s) 1015, 1322, 1383, 2436
EarI CTCTTC 4 cut(s) 1015, 1322, 1383, 2436
Eco130I CCWWGG 2 cut(s) 1650, 1973
Eco32I GATATC 1 cut(s) 707
Eco47I GGWCC 1 cut(s) 1592
Eco57I CTGAAG 5 cut(s) 285, 1287, 1694, 2352, 2464
Eco72I CACGTG 1 cut(s) 2456
Eco88I CYCGRG 1 cut(s) 1906
EcoNI CCTNNNNNAGG 1 cut(s) 591
EcoRII CCWGG 5 cut(s) 511, 566, 1115, 1992, 2407
EcoRV GATATC 1 cut(s) 707
EcoT14I CCWWGG 2 cut(s) 1650, 1973
EcoT22I ATGCAT 1 cut(s) 2209
EgeI GGCGCC 1 cut(s) 2067
EheI GGCGCC 1 cut(s) 2067
ErhI CCWWGG 2 cut(s) 1650, 1973
FaqI GGGAC 1 cut(s) 1590
FauNDI CATATG 2 cut(s) 823, 2053
FbaI TGATCA 4 cut(s) 37, 322, 1816, 2014
FblI GTMKAC 1 cut(s) 794
Fnu4HI GCNGC 4 cut(s) 1222, 2321, 2390, 2405
FokI GGATG 4 cut(s) 625, 1041, 1490, 2160
Fsp4HI GCNGC 4 cut(s) 1222, 2321, 2390, 2405
FspBI CTAG 7 cut(s) 122, 167, 456, 669, 804, 1941, 1955
GlaI GCGC 1 cut(s) 2067
GluI GCNGC 4 cut(s) 1222, 2321, 2390, 2405
GsuI CTGGAG 1 cut(s) 589
HaeII RGCGCY 1 cut(s) 2069
HaeIII GGCC 6 cut(s) 219, 528, 690, 718, 1846, 1992
HapII CCGG 4 cut(s) 287, 529, 790, 2267
HhaI GCGC 1 cut(s) 2068
Hin1I GRCGYC 1 cut(s) 2066
Hin6I GCGC 1 cut(s) 2066
HinP1I GCGC 1 cut(s) 2066
HincII GTYRAC 2 cut(s) 372, 843
HindII GTYRAC 2 cut(s) 372, 843
HindIII AAGCTT 1 cut(s) 1730
HpaI GTTAAC 1 cut(s) 843
HpaII CCGG 4 cut(s) 287, 529, 790, 2267
HphI GGTGA 3 cut(s) 170, 1889, 2460
Hpy166II GTNNAC 9 cut(s) 240, 372, 629, 777, 795, 843, 1179, 1600, 2468
Hpy8I GTNNAC 9 cut(s) 240, 372, 629, 777, 795, 843, 1179, 1600, 2468
Hpy99I CGWCG 1 cut(s) 2101
HpyAV CCTTC 7 cut(s) 350, 701, 1032, 1202, 1484, 1488, 1857
HpyCH4III ACNGT 5 cut(s) 364, 837, 1484, 1534, 1718
HpyCH4IV ACGT 2 cut(s) 797, 2455
HpyF10VI GCNNNNNNNGC 6 cut(s) 125, 357, 890, 1843, 2395, 2404
HpyF3I CTNAG 4 cut(s) 535, 657, 732, 1436
HpySE526I ACGT 2 cut(s) 797, 2455
Hsp92I GRCGYC 1 cut(s) 2066
HspAI GCGC 1 cut(s) 2066
KasI GGCGCC 1 cut(s) 2065
Kpn2I TCCGGA 1 cut(s) 2266
Ksp22I TGATCA 4 cut(s) 37, 322, 1816, 2014
KspAI GTTAAC 1 cut(s) 843
LguI GCTCTTC 2 cut(s) 1322, 1383
LmnI GCTCC 4 cut(s) 573, 653, 871, 1794
Lsp1109I GCAGC 4 cut(s) 1208, 2332, 2376, 2416
LweI GCATC 3 cut(s) 115, 1303, 1531
MaeI CTAG 7 cut(s) 122, 167, 456, 669, 804, 1941, 1955
MaeII ACGT 2 cut(s) 797, 2455
MaeIII GTNAC 5 cut(s) 497, 1509, 2451, 2456, 2474
MfeI CAATTG 1 cut(s) 695
MflI RGATCY 2 cut(s) 616, 1930
MhlI GDGCHC 1 cut(s) 578
Mly113I GGCGCC 1 cut(s) 2066
MlyI GAGTC 5 cut(s) 315, 1036, 1898, 2215, 2422
MmeI TCCRAC 2 cut(s) 1362, 1812
Mph1103I ATGCAT 1 cut(s) 2209
MroI TCCGGA 1 cut(s) 2266
MroXI GAANNNNTTC 1 cut(s) 1297
MslI CAYNNNNRTG 2 cut(s) 260, 1000
MspI CCGG 4 cut(s) 287, 529, 790, 2267
MspR9I CCNGG 5 cut(s) 513, 568, 1117, 1994, 2409
MunI CAATTG 1 cut(s) 695
Mva1269I GAATGC 3 cut(s) 1151, 1398, 1746
MvaI CCWGG 5 cut(s) 513, 568, 1117, 1994, 2409
MwoI GCNNNNNNNGC 6 cut(s) 125, 357, 890, 1843, 2395, 2404
NarI GGCGCC 1 cut(s) 2066
NdeI CATATG 2 cut(s) 823, 2053
NlaIV GGNNCC 3 cut(s) 618, 873, 2067
NmuCI GTSAC 3 cut(s) 497, 2451, 2456
NsiI ATGCAT 1 cut(s) 2209
NspI RCATGY 4 cut(s) 969, 1786, 2207, 2350
NspV TTCGAA 1 cut(s) 1036
OliI CACNNNNGTG 1 cut(s) 1000
PaeI GCATGC 1 cut(s) 969
PaeR7I CTCGAG 1 cut(s) 1906
PagI TCATGA 1 cut(s) 2377
PciI ACATGT 1 cut(s) 1782
PciSI GCTCTTC 2 cut(s) 1322, 1383
PctI GAATGC 3 cut(s) 1151, 1398, 1746
PdmI GAANNNNTTC 1 cut(s) 1297
PfeI GAWTC 8 cut(s) 198, 296, 419, 817, 1271, 1407, 1655, 2438
PflMI CCANNNNNTGG 1 cut(s) 226
PfoI TCCNGGA 1 cut(s) 566
PinAI ACCGGT 2 cut(s) 286, 789
PkrI GCNGC 4 cut(s) 1223, 2322, 2391, 2406
PleI GAGTC 5 cut(s) 314, 1036, 1898, 2215, 2422
PluTI GGCGCC 1 cut(s) 2069
PmaCI CACGTG 1 cut(s) 2456
PmlI CACGTG 1 cut(s) 2456
PpsI GAGTC 5 cut(s) 314, 1036, 1898, 2215, 2422
Ppu21I YACGTR 1 cut(s) 2456
PscI ACATGT 1 cut(s) 1782
Psp6I CCWGG 5 cut(s) 511, 566, 1115, 1992, 2407
PspCI CACGTG 1 cut(s) 2456
PspGI CCWGG 5 cut(s) 511, 566, 1115, 1992, 2407
PspN4I GGNNCC 3 cut(s) 618, 873, 2067
PspPI GGNCC 1 cut(s) 1592
PstI CTGCAG 1 cut(s) 394
PsuI RGATCY 2 cut(s) 616, 1930
RsaI GTAC 9 cut(s) 239, 776, 984, 1180, 1601, 1609, 2232, 2328, 2361
RsaNI GTAC 9 cut(s) 238, 775, 983, 1179, 1600, 1608, 2231, 2327, 2360
RseI CAYNNNNRTG 2 cut(s) 260, 1000
SapI GCTCTTC 2 cut(s) 1322, 1383
SatI GCNGC 4 cut(s) 1222, 2321, 2390, 2405
Sau96I GGNCC 1 cut(s) 1592
SchI GAGTC 5 cut(s) 315, 1036, 1898, 2215, 2422
ScrFI CCNGG 5 cut(s) 513, 568, 1117, 1994, 2409
SduI GDGCHC 1 cut(s) 578
SfaNI GCATC 3 cut(s) 115, 1303, 1531
SfcI CTRYAG 2 cut(s) 390, 1533
SfoI GGCGCC 1 cut(s) 2067
Sfr274I CTCGAG 1 cut(s) 1906
SfuI TTCGAA 1 cut(s) 1036
SinI GGWCC 1 cut(s) 1592
SlaI CTCGAG 1 cut(s) 1906
SmiI ATTTAAAT 1 cut(s) 1093
SmiMI CAYNNNNRTG 2 cut(s) 260, 1000
SmlI CTYRAG 4 cut(s) 591, 1139, 1275, 1906
SmoI CTYRAG 4 cut(s) 591, 1139, 1275, 1906
SphI GCATGC 1 cut(s) 969
SsiI CCGC 2 cut(s) 1514, 1584
SspDI GGCGCC 1 cut(s) 2065
SspI AATATT 1 cut(s) 1948
SspMI CTAG 7 cut(s) 122, 167, 456, 669, 804, 1941, 1955
StyD4I CCNGG 5 cut(s) 511, 566, 1115, 1992, 2407
StyI CCWWGG 2 cut(s) 1650, 1973
SwaI ATTTAAAT 1 cut(s) 1093
TaaI ACNGT 5 cut(s) 364, 837, 1484, 1534, 1718
TaiI ACGT 2 cut(s) 800, 2458
TaqI TCGA 6 cut(s) 212, 1036, 1839, 1907, 2096, 2247
TatI WGTACW 2 cut(s) 774, 1599
TfiI GAWTC 8 cut(s) 198, 296, 419, 817, 1271, 1407, 1655, 2438
TseFI GTSAC 3 cut(s) 497, 2451, 2456
TseI GCWGC 4 cut(s) 1221, 2320, 2389, 2404
Tsp45I GTSAC 3 cut(s) 497, 2451, 2456
TspGWI ACGGA 5 cut(s) 85, 625, 1438, 1884, 2440
Van91I CCANNNNNTGG 1 cut(s) 226
VpaK11BI GGWCC 1 cut(s) 1592
XagI CCTNNNNNAGG 1 cut(s) 591
XapI RAATTY 4 cut(s) 906, 1192, 1259, 2088
XbaI TCTAGA 1 cut(s) 803
XceI RCATGY 4 cut(s) 969, 1786, 2207, 2350
XcmI CCANNNNNNNNNTGG 1 cut(s) 1795
XhoI CTCGAG 1 cut(s) 1906
XmiI GTMKAC 1 cut(s) 794
XmnI GAANNNNTTC 1 cut(s) 1297
XspI CTAG 7 cut(s) 122, 167, 456, 669, 804, 1941, 1955
Zsp2I ATGCAT 1 cut(s) 2209
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.