MD05G1214700.v1.1

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0000207
Physical Location & Seq
Reverse (-)
34570863 .. 34573993
3131 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1214700.v1.1.491

Sequence Viewer

Length: 2472 bp
ATGCAAGCCTTGACAAGTGTTTTTATGTGCTTGTTCTTCTTATTCTCCCTCCAAAGAATCTCAGCTGCAACTGCACTGGTCACGATCACTCCGAGTGGATATATAAGAGATGGCGAAACTCTGGTTTCAGTAGGTGGAAGCTATGAACTGGGATTCTTCAGCCCTGGTAAATCGAAAAGCCGGTACTTGGGAATATGGTACACTGCTTCTGCTGATGCAGTTGTGTGGGTGGCCAACAGAGAAACACCACTTGGTGATTCTTCAGGACTTTTGAAGCTCACGGAGCAAGGAGTTCTAGTCCTCCTGAATAGCTCAAACAGCATTGTGTGGTCATCGAATTCATCAAGAATTGCGGGGAATCCAGTATCGCAACTATTGGATTCTGGAAATCTTGTGGTGCAAGATGGGAAAGAAACTAACCCGGATAACTTCTTGTGGCAGAGTTTTGATTATCCTTGTGACACATACCTACCGGAAATGAAGCTTGGTTGGGACTTGGTTACCGGTTTAGAGAGGTATCTCTCGTCTTGGAAGAGCACAGAAGATCCTTCTCCGGGAGCGTTTTCGGGACGAGTGGATCTGCGTGGCTTACCACAATTTTTAATTATGAAAGGAGATAAGATACAAGCCAGACGAGGGTCATGGAATGGCCTTTATTTGACAGGATACGGATATGATGTCACACATCCAGATCCAATATCTGGGACTGAATTTGTGATGAACAAGGACGAGGTCTATTATGAGTACAGGCTCCGGAACATGTCAGTGTTCTCGAGATATGTATTGAACCCATCAGGAGTCTCAGAGAAGTTCACATGGGTGTCCCAAACACAGAGTTGGGAACTTGCCTTTTCATTCCTAGCAGATCGGTGTGAAAAGTATGCCTTGTGCGGTCCATTTTCTAGTTGCAGTATCAATAGTAACTCTGCAATATGTGCATGCATGAAGGGATTTGTACCAAAATCTTTGAAAGATTGGAATTCGGGATATTGGTCTGATGGATGTGTTCGAAGGACTCCGTTGGCCTGCAGCGATGGAGATGGCTTCGTAAAGTATAGTGGGATTAAATTGCCAGACACATCCTCCTCATGGTTTGATAAAAGCATGAGCCTTAGTGAATGCAAGGGATTATGTTGGGGAAACTGCTCATGCACGGCGTGTGCAAATCTAGACATCAGGGAGGGAGGAAGTGGCTGCGTGCTTTGGTTTGGTAACCTCATCGACATAAAAGAATTCACTCCCGGCGGTGGTCAAGACCTCTATATACGGGTGGCAGCTTCAGAACTAGATCATGTTAAGAAAAAGAGCAATTTCAACAAGAGACAGCTACTAGCAATTCTCCTCAGCTCTGCAGTTTTTCTCGTGGGATTGCTTGTTGGAATGATATTGTACATACGGATGAAGAAACTCAGATATGAAGGACACAGAAGAAAGGATTGCAGAGACGAATACCTTGGAGAAGACGGGGAAGAGATGGAGTTACCACTGTTTGACTTGACCACCATAGCTAAAGCCACTGACAACTTTTCAAGCTGTAACAAGCTGGGAGAAGGTGGCTTTGGACCTGTGTACAAGGGTACCCTGATAGGAGGAAAAGAAATTGCAGTAAAGAGGCTTTCAAAGAATTCCGGACAAGGAATGAAAGAGTTCAAAACTGAAGTTATACTCATAGCCAGACTTCAGCATCGCAATCTTGTTAAGCTTCTCGGTTGTTGCATTCAAAAAGATGAAAAGATTCTAATATATGAATTCATGCCCAACAAAAGCTTGGACTTCTATATTTTTGATCAGGAGGGACAAAAATTGCTCGATTGGCCTAAGTGCTTCCACATTATCAGTGGAGTTGCTCGAGGGCTTCTTTATCTTCACCAAGACTCTAGATTAAGGATTATACATCGAGATTTGAAATGTAGTAATATTTTGCTAGATAATAATATGAACCCGAAGATTTCAGACTTCGGCCTGGCTAAAACATTCGGCGGTGACCAAAGTCAGGCCAATACAAACAGAGTGGTTGGAACCTATGGTTATATGTCTCCGGAATATACAGCAGATGGAATTTTCTCGATGAAATCTGATGTGTTTAGCTTTGGAGTCATATTGCTCGAGATGTTGAGTAGGAAGAAGAACAGGGGATTTCGTCATCCAGATCACAACCACAACCTTCTTGGACATGCATGGATACTATGGATTCAGGATAAACCACTAGAACTGATCGATAAGAAGTTTTGTGATTTGTGCAACATATATGAAGTGGTAAGGTGTCTTCACGTGGGGCTATTATGTGTGCAAAGAGTACCTGAGGATAGACCAAACATGTCATCTGTGGTTCTAATGTTGAGCAGTGATGTTGCCTTGCCGCCTCCAAAGCAGCCCGGTTTTTACACTGAACAAAGCATCCCTGAATCACCATCAAGGATGCTTCTTTGTTCAGAGAATACTTTCAGCACTGCGTTGATAGATCCTCGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

824

Amino Acids

92.09

Weight (kDa)

7.65

Isoelectric Point (pI)

41.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 74 - 178 4.1e-38 D-mannose binding lectin
S_locus_glycop PF00954 211 - 322 1.4e-23 S-locus glycoprotein domain
PAN_2 PF08276 343 - 409 6.6e-19 PAN-like domain
Pkinase PF00069 511 - 712 2.4e-44 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 513 - 711 4.6e-45 Protein tyrosine and serine/threonine kinase
DUF3403 PF11883 781 - 823 1.4e-06 Domain of unknown function (DUF3403)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000207)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G27300
fragaria_vesca FvH4_3g15930 FvH4_3g15980 FvH4_3g15980 FvH4_3g15980 FvH4_3g15980 FvH4_3g21350 FvH4_3g21400 FvH4_3g21400 FvH4_6g12332
malus_domestica MD02G1167100.v1.1 MD02G1167200.v1.1 MD03G1185600.v1.1 MD05G1213900.v1.1 MD05G1214100.v1.1 MD05G1214200.v1.1 MD05G1214700.v1.1 MD05G1216300.v1.1 MD05G1216800.v1.1 MD05G1217300.v1.1 MD05G1218000.v1.1 MD11G1231100.v1.1 MD11G1231200.v1.1 MD11G1231400.v1.1 MD11G1231500.v1.1 MD11G1232400.v1.1 MD11G1232500.v1.1
prunus_persica Prupe.4G142300_v2.0.a1 Prupe.4G142400_v2.0.a1 Prupe.4G142500_v2.0.a1 Prupe.4G142600_v2.0.a1 Prupe.4G142800_v2.0.a1 Prupe.4G195000_v2.0.a1 Prupe.4G195000_v2.0.a1 Prupe.4G195000_v2.0.a1 Prupe.4G195100_v2.0.a1 Prupe.4G195300_v2.0.a1 Prupe.4G195300_v2.0.a1 Prupe.4G195300_v2.0.a1 Prupe.4G195400_v2.0.a1 Prupe.4G195400_v2.0.a1 Prupe.4G195500_v2.0.a1 Prupe.4G195700_v2.0.a1 Prupe.4G195900_v2.0.a1
pyrus_communis pycom03g16540 pycom05g19920 pycom11g20460 pycom11g20480 pycom11g20490 pycom11g20500 pycom11g20530
rosa_chinensis RchiOBHm_Chr2g0139311 RchiOBHm_Chr2g0139351 RchiOBHm_Chr5g0026801 RchiOBHm_Chr5g0026851 RchiOBHm_Chr5g0026891 RchiOBHm_Chr5g0026911 RchiOBHm_Chr5g0036571 RchiOBHm_Chr5g0036591 RchiOBHm_Chr5g0036721 RchiOBHm_Chr5g0036741 RchiOBHm_Chr7g0222981
rosa_laevigata RLG00000002006 RLG00000019807 RLG00000032964 RLG00000032968 RLG00000032969 RLG00000033706 RLG00000033708 RLG00000033712 RLG00000033716
rosa_multiflora Rmu_co8256651.1_g000001 Rmu_co8279377.1_g000001 Rmu_co8429919.1_g000001 Rmu_co8490103.1_g000001 Rmu_sc0000774.1_g000002 Rmu_sc0002525.1_g000012 Rmu_sc0004223.1_g000008 Rmu_sc0004390.1_g000002 Rmu_sc0006059.1_g000014 Rmu_sc0006059.1_g000024 Rmu_sc0006514.1_g000020 Rmu_sc0014333.1_g000002 Rmu_ssc0000172.1_g000018 Rmu_ssc0000172.1_g000029
rosa_roxburghii Rroxscaffold_1G00044230 Rroxscaffold_1G00044260 Rroxscaffold_1G00044320 Rroxscaffold_1G00044340 Rroxscaffold_1G00052560 Rroxscaffold_1G00052570 Rroxscaffold_1G00052660 Rroxscaffold_1G00052720 Rroxscaffold_2G00105450 Rroxscaffold_2G00105460
rosa_rugosa Rorug02G0356900 Rorug02G0357000 Rorug02G0357100 Rorug02G0357200 Rorug02G0357300 Rorug02G0357400 Rorug02G0357500 Rorug02G0357600 Rorug05G0095400 Rorug05G0095500 Rorug05G0095500 Rorug05G0095600 Rorug05G0095700 Rorug05G0095800 Rorug05G0157700 Rorug05G0157800
rosa_samantha Rh2AG406800 Rh2AG406900 Rh2BG417300 Rh2CG392900 Rh2DG426200 Rh5AG189300 Rh5AG249400 Rh5AG250100 Rh5BG186300 Rh5BG186900 Rh5BG187000 Rh5BG249500 Rh5BG250600 Rh5BG250900 Rh5BG251500 Rh5BG251800 Rh5CG205500 Rh5CG206300 Rh5CG206700 Rh5CG283000 Rh5CG283300 Rh5CG283500 Rh5DG188500 Rh5DG188700 Rh5DG258700 Rh5DG258900 Rh5DG259600 Rh5DG259900 Rh7CG375800 Rh7DG352500
rosa_wichuraiana Rw2G032960 Rw2G032970 Rw2G033220 Rw2G033230 Rw5G017130 Rw5G017230 Rw5G022940 Rw5G022960 Rw5G022980 Rw7G030420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1577
AccB1I GGYRCC 1 cut(s) 1577
AccB7I CCANNNNNTGG 1 cut(s) 701
AccIII TCCGGA 3 cut(s) 753, 1628, 2038
AciI CCGC 5 cut(s) 353, 891, 1245, 1980, 2360
AclWI GGATC 4 cut(s) 539, 585, 686, 2456
AcoI YGGCCR 1 cut(s) 231
AcsI RAATTY 7 cut(s) 337, 710, 979, 1232, 1624, 1748, 2058
AcuI CTGAAG 5 cut(s) 142, 246, 1263, 1664, 1677
AcvI CACGTG 1 cut(s) 2272
AdeI CACNNNGTG 2 cut(s) 254, 1158
AfaI GTAC 8 cut(s) 185, 200, 746, 957, 1391, 1571, 1579, 2298
AfiI CCNNNNNNNGG 7 cut(s) 187, 554, 636, 701, 1089, 1247, 1993
AflIII ACRYGT 2 cut(s) 759, 2316
AgeI ACCGGT 1 cut(s) 503
AgsI TTSAA 9 cut(s) 274, 787, 970, 1315, 1530, 1620, 1651, 1721, 1906
AjnI CCWGG 2 cut(s) 163, 1962
AjuI GAANNNNNNNTTGG 6 cut(s) 234, 266, 468, 500, 1542, 1574
AleI CACNNNNGTG 1 cut(s) 818
AloI GAACNNNNNNTCC 2 cut(s) 2382, 2414
Alw21I GWGCWC 1 cut(s) 539
Alw26I GTCTC 4 cut(s) 805, 1315, 1437, 2040
AlwI GGATC 4 cut(s) 539, 585, 686, 2456
Ama87I CYCGRG 3 cut(s) 772, 1848, 2105
Aor13HI TCCGGA 3 cut(s) 753, 1628, 2038
AoxI GGCC 6 cut(s) 231, 649, 1023, 1814, 1960, 1995
ApeKI GCWGC 5 cut(s) 65, 1029, 1194, 1274, 2371
ApoI RAATTY 7 cut(s) 337, 710, 979, 1232, 1624, 1748, 2058
AsiGI ACCGGT 1 cut(s) 503
Asp700I GAANNNNTTC 3 cut(s) 1646, 1734, 2441
Asp718I GGTACC 1 cut(s) 1577
AspS9I GGNCC 2 cut(s) 893, 1562
AsuC2I CCSGG 4 cut(s) 422, 555, 1242, 2376
AsuHPI GGTGA 4 cut(s) 266, 1859, 1994, 2400
AsuII TTCGAA 1 cut(s) 1009
AvaI CYCGRG 3 cut(s) 772, 1848, 2105
AvaII GGWCC 2 cut(s) 893, 1562
AxyI CCTNAGG 1 cut(s) 2301
BaeI ACNNNNGTAYC 2 cut(s) 1561, 1594
BalI TGGCCA 1 cut(s) 233
BanI GGYRCC 1 cut(s) 1577
BarI GAAGNNNNNNTAC 2 cut(s) 2250, 2282
BauI CACGAG 1 cut(s) 1361
BbrPI CACGTG 1 cut(s) 2272
BbsI GAAGAC 2 cut(s) 1467, 2258
Bbv12I GWGCWC 1 cut(s) 539
BbvCI CCTCAGC 1 cut(s) 1343
BbvI GCAGC 5 cut(s) 52, 1041, 1181, 1286, 2383
BccI CCATC 9 cut(s) 104, 398, 799, 992, 1028, 1034, 1468, 2048, 2419
BceAI ACGGC 1 cut(s) 1170
BciT130I CCWGG 2 cut(s) 165, 1964
BciVI GTATCC 2 cut(s) 659, 2175
BclI TGATCA 1 cut(s) 1786
BcnI CCSGG 4 cut(s) 422, 555, 1242, 2376
BcoDI GTCTC 4 cut(s) 805, 1315, 1437, 2040
BfaI CTAG 9 cut(s) 296, 860, 903, 1169, 1286, 1331, 1878, 1925, 2207
BfmI CTRYAG 2 cut(s) 1027, 1350
BfuI GTATCC 2 cut(s) 659, 2175
BisI GCNGC 6 cut(s) 66, 1030, 1195, 1275, 2360, 2372
BlsI GCNGC 6 cut(s) 67, 1031, 1196, 1276, 2361, 2373
Bme1390I CCNGG 6 cut(s) 165, 422, 555, 1242, 1964, 2376
Bme18I GGWCC 2 cut(s) 893, 1562
BmeT110I CYCGRG 3 cut(s) 772, 1848, 2105
BmgT120I GGNCC 2 cut(s) 893, 1562
BmiI GGNNCC 3 cut(s) 752, 1579, 2020
BmrFI CCNGG 6 cut(s) 165, 422, 555, 1242, 1964, 2376
BmrI ACTGGG 1 cut(s) 158
BmsI GCATC 4 cut(s) 205, 1693, 2406, 2409
BmuI ACTGGG 1 cut(s) 158
BoxI GACNNNNGTC 2 cut(s) 637, 1989
BpiI GAAGAC 2 cut(s) 1467, 2258
Bpu10I CCTNAGC 1 cut(s) 1343
Bpu14I TTCGAA 1 cut(s) 1009
BpuMI CCSGG 4 cut(s) 422, 555, 1242, 2376
Bsa29I ATCGAT 1 cut(s) 2217
BsaAI YACGTR 1 cut(s) 2272
BsaJI CCNNGG 3 cut(s) 163, 1453, 2465
BsaWI WCCGGW 5 cut(s) 472, 503, 753, 1628, 2038
BsaXI ACNNNNNCTCC 6 cut(s) 73, 103, 282, 312, 1067, 1097
Bsc4I CCNNNNNNNGG 7 cut(s) 187, 554, 636, 701, 1089, 1247, 1993
Bse118I RCCGGY 2 cut(s) 180, 503
Bse1I ACTGG 3 cut(s) 81, 153, 362
Bse21I CCTNAGG 1 cut(s) 2301
BseAI TCCGGA 3 cut(s) 753, 1628, 2038
BseBI CCWGG 2 cut(s) 165, 1964
BseCI ATCGAT 1 cut(s) 2217
BseDI CCNNGG 3 cut(s) 163, 1453, 2465
BseGI GGATG 7 cut(s) 685, 1007, 1079, 1404, 2143, 2397, 2424
BseLI CCNNNNNNNGG 7 cut(s) 187, 554, 636, 701, 1089, 1247, 1993
BseMII CTCAG 5 cut(s) 75, 816, 1357, 1423, 2292
BseNI ACTGG 3 cut(s) 81, 153, 362
BseRI GAGGAG 2 cut(s) 1075, 1331
BseXI GCAGC 5 cut(s) 52, 1041, 1181, 1286, 2383
BseYI CCCAGC 1 cut(s) 1543
BsgI GTGCAG 1 cut(s) 57
BshFI GGCC 6 cut(s) 233, 651, 1025, 1816, 1962, 1997
BshNI GGYRCC 1 cut(s) 1577
BshTI ACCGGT 1 cut(s) 503
BshVI ATCGAT 1 cut(s) 2217
BsiHKAI GWGCWC 1 cut(s) 539
BsiHKCI CYCGRG 3 cut(s) 772, 1848, 2105
BslFI GGGAC 5 cut(s) 506, 582, 718, 808, 1809
BslI CCNNNNNNNGG 7 cut(s) 187, 554, 636, 701, 1089, 1247, 1993
BsmAI GTCTC 4 cut(s) 805, 1315, 1437, 2040
BsmBI CGTCTC 1 cut(s) 1437
BsmFI GGGAC 5 cut(s) 506, 582, 718, 808, 1809
BsmI GAATGC 2 cut(s) 1124, 1716
BsnI GGCC 6 cut(s) 233, 651, 1025, 1816, 1962, 1997
BsoBI CYCGRG 3 cut(s) 772, 1848, 2105
Bsp119I TTCGAA 1 cut(s) 1009
Bsp1286I GDGCHC 1 cut(s) 539
Bsp13I TCCGGA 3 cut(s) 753, 1628, 2038
Bsp1407I TGTACA 2 cut(s) 1389, 1569
BspACI CCGC 5 cut(s) 353, 891, 1245, 1980, 2360
BspANI GGCC 6 cut(s) 233, 651, 1025, 1816, 1962, 1997
BspCNI CTCAG 5 cut(s) 74, 815, 1356, 1422, 2293
BspDI ATCGAT 1 cut(s) 2217
BspEI TCCGGA 3 cut(s) 753, 1628, 2038
BspLI GGNNCC 3 cut(s) 752, 1579, 2020
BspMAI CTGCAG 2 cut(s) 1031, 1354
BspPI GGATC 4 cut(s) 539, 585, 686, 2456
BspQI GCTCTTC 1 cut(s) 527
BspT104I TTCGAA 1 cut(s) 1009
BspT107I GGYRCC 1 cut(s) 1577
BsrFI RCCGGY 2 cut(s) 180, 503
BsrGI TGTACA 2 cut(s) 1389, 1569
BsrI ACTGG 3 cut(s) 81, 153, 362
BssAI RCCGGY 2 cut(s) 180, 503
BssECI CCNNGG 3 cut(s) 163, 1453, 2465
BssSI CACGAG 1 cut(s) 1361
BssT1I CCWWGG 1 cut(s) 1453
Bst2BI CACGAG 1 cut(s) 1361
Bst2UI CCWGG 2 cut(s) 165, 1964
Bst4CI ACNGT 1 cut(s) 1488
Bst6I CTCTTC 2 cut(s) 527, 1464
BstAPI GCANNNNNTGC 1 cut(s) 935
BstAUI TGTACA 2 cut(s) 1389, 1569
BstBAI YACGTR 1 cut(s) 2272
BstBI TTCGAA 1 cut(s) 1009
BstC8I GCNNGC 4 cut(s) 6, 940, 1027, 1199
BstDEI CTNAG 7 cut(s) 61, 802, 1112, 1343, 1409, 1818, 2301
BstEII GGTNACC 3 cut(s) 499, 1211, 1982
BstF5I GGATG 7 cut(s) 685, 1007, 1079, 1404, 2143, 2397, 2424
BstMAI GTCTC 4 cut(s) 805, 1315, 1437, 2040
BstMWI GCNNNNNNNGC 6 cut(s) 71, 283, 318, 935, 1813, 2368
BstNI CCWGG 2 cut(s) 165, 1964
BstNSI RCATGY 4 cut(s) 763, 942, 2177, 2320
BstPAI GACNNNNGTC 2 cut(s) 637, 1989
BstPI GGTNACC 3 cut(s) 499, 1211, 1982
BstSCI CCNGG 6 cut(s) 163, 420, 553, 1240, 1962, 2374
BstSFI CTRYAG 2 cut(s) 1027, 1350
BstV1I GCAGC 5 cut(s) 52, 1041, 1181, 1286, 2383
BstV2I GAAGAC 2 cut(s) 1467, 2258
BstX2I RGATCY 4 cut(s) 544, 577, 691, 2461
BstYI RGATCY 4 cut(s) 544, 577, 691, 2461
Bsu15I ATCGAT 1 cut(s) 2217
Bsu36I CCTNAGG 1 cut(s) 2301
BsuI GTATCC 2 cut(s) 659, 2175
BsuRI GGCC 6 cut(s) 233, 651, 1025, 1816, 1962, 1997
BsuTUI ATCGAT 1 cut(s) 2217
BtgZI GCGATG 2 cut(s) 1047, 1670
BtsCI GGATG 7 cut(s) 685, 1007, 1079, 1404, 2143, 2397, 2424
BtsI GCAGTG 3 cut(s) 201, 2350, 2448
BtsIMutI CAGTG 9 cut(s) 74, 201, 771, 1484, 1515, 1843, 2350, 2385, 2448
Cac8I GCNNGC 4 cut(s) 6, 940, 1027, 1199
Cfr10I RCCGGY 2 cut(s) 180, 503
Cfr13I GGNCC 2 cut(s) 893, 1562
ClaI ATCGAT 1 cut(s) 2217
Csp6I GTAC 8 cut(s) 184, 199, 745, 956, 1390, 1570, 1578, 2297
CspAI ACCGGT 1 cut(s) 503
CspCI CAANNNNNGTGG 2 cut(s) 1992, 2027
CviQI GTAC 8 cut(s) 184, 199, 745, 956, 1390, 1570, 1578, 2297
DdeI CTNAG 7 cut(s) 61, 802, 1112, 1343, 1409, 1818, 2301
DraIII CACNNNGTG 2 cut(s) 254, 1158
EaeI YGGCCR 1 cut(s) 231
Eam1104I CTCTTC 2 cut(s) 527, 1464
EarI CTCTTC 2 cut(s) 527, 1464
Eco130I CCWWGG 1 cut(s) 1453
Eco47I GGWCC 2 cut(s) 893, 1562
Eco57I CTGAAG 5 cut(s) 142, 246, 1263, 1664, 1677
Eco72I CACGTG 1 cut(s) 2272
Eco81I CCTNAGG 1 cut(s) 2301
Eco88I CYCGRG 3 cut(s) 772, 1848, 2105
Eco91I GGTNACC 3 cut(s) 499, 1211, 1982
EcoO65I GGTNACC 3 cut(s) 499, 1211, 1982
EcoRI GAATTC 5 cut(s) 337, 979, 1232, 1624, 1748
EcoRII CCWGG 2 cut(s) 163, 1962
EcoT14I CCWWGG 1 cut(s) 1453
EcoT22I ATGCAT 2 cut(s) 944, 2179
ErhI CCWWGG 1 cut(s) 1453
Esp3I CGTCTC 1 cut(s) 1437
FalI AAGNNNNNCTT 2 cut(s) 869, 901
FaqI GGGAC 5 cut(s) 506, 582, 718, 808, 1809
FauI CCCGC 1 cut(s) 346
FbaI TGATCA 1 cut(s) 1786
Fnu4HI GCNGC 6 cut(s) 66, 1030, 1195, 1275, 2360, 2372
FokI GGATG 7 cut(s) 672, 1014, 1066, 1411, 2130, 2384, 2431
Fsp4HI GCNGC 6 cut(s) 66, 1030, 1195, 1275, 2360, 2372
FspBI CTAG 9 cut(s) 296, 860, 903, 1169, 1286, 1331, 1878, 1925, 2207
GluI GCNGC 6 cut(s) 66, 1030, 1195, 1275, 2360, 2372
GsaI CCCAGC 1 cut(s) 1547
HaeIII GGCC 6 cut(s) 233, 651, 1025, 1816, 1962, 1997
HindIII AAGCTT 3 cut(s) 482, 1700, 1765
HphI GGTGA 4 cut(s) 266, 1859, 1994, 2400
Hpy166II GTNNAC 3 cut(s) 201, 813, 1570
Hpy188I TCNGA 8 cut(s) 93, 805, 997, 1282, 1412, 1954, 2077, 2434
Hpy8I GTNNAC 3 cut(s) 201, 813, 1570
HpyAV CCTTC 6 cut(s) 558, 940, 1005, 1412, 1544, 2174
HpyCH4III ACNGT 1 cut(s) 1488
HpyCH4IV ACGT 1 cut(s) 2271
HpyF10VI GCNNNNNNNGC 6 cut(s) 71, 283, 318, 935, 1813, 2368
HpyF3I CTNAG 7 cut(s) 61, 802, 1112, 1343, 1409, 1818, 2301
HpySE526I ACGT 1 cut(s) 2271
Kpn2I TCCGGA 3 cut(s) 753, 1628, 2038
KpnI GGTACC 1 cut(s) 1581
Ksp22I TGATCA 1 cut(s) 1786
LguI GCTCTTC 1 cut(s) 527
LmnI GCTCC 3 cut(s) 283, 557, 756
Lsp1109I GCAGC 5 cut(s) 52, 1041, 1181, 1286, 2383
LweI GCATC 4 cut(s) 205, 1693, 2406, 2409
MaeI CTAG 9 cut(s) 296, 860, 903, 1169, 1286, 1331, 1878, 1925, 2207
MaeII ACGT 1 cut(s) 2271
MaeIII GTNAC 9 cut(s) 79, 458, 499, 679, 920, 1211, 1479, 1535, 1982
MflI RGATCY 4 cut(s) 544, 577, 691, 2461
MhlI GDGCHC 1 cut(s) 539
MlsI TGGCCA 1 cut(s) 233
MluNI TGGCCA 1 cut(s) 233
MlyI GAGTC 4 cut(s) 807, 1009, 1868, 2103
MmeI TCCRAC 2 cut(s) 1357, 1996
Mox20I TGGCCA 1 cut(s) 233
Mph1103I ATGCAT 2 cut(s) 944, 2179
MroI TCCGGA 3 cut(s) 753, 1628, 2038
MroXI GAANNNNTTC 3 cut(s) 1646, 1734, 2441
MscI TGGCCA 1 cut(s) 233
MseI TTAA 5 cut(s) 602, 1065, 1296, 1698, 1883
MslI CAYNNNNRTG 3 cut(s) 764, 818, 1397
Msp20I TGGCCA 1 cut(s) 233
MspA1I CMGCKG 1 cut(s) 65
MspR9I CCNGG 6 cut(s) 165, 422, 555, 1242, 1964, 2376
Mva1269I GAATGC 2 cut(s) 1124, 1716
MvaI CCWGG 2 cut(s) 165, 1964
MwoI GCNNNNNNNGC 6 cut(s) 71, 283, 318, 935, 1813, 2368
NciI CCSGG 4 cut(s) 422, 555, 1242, 2376
NlaIV GGNNCC 3 cut(s) 752, 1579, 2020
NmuCI GTSAC 4 cut(s) 79, 458, 679, 1982
NsiI ATGCAT 2 cut(s) 944, 2179
NspI RCATGY 4 cut(s) 763, 942, 2177, 2320
NspV TTCGAA 1 cut(s) 1009
OliI CACNNNNGTG 1 cut(s) 818
PaeI GCATGC 1 cut(s) 942
PaeR7I CTCGAG 3 cut(s) 772, 1848, 2105
PciI ACATGT 2 cut(s) 759, 2316
PciSI GCTCTTC 1 cut(s) 527
PcsI WCGNNNNNNNCGW 1 cut(s) 89
PctI GAATGC 2 cut(s) 1124, 1716
PdmI GAANNNNTTC 3 cut(s) 1646, 1734, 2441
PfeI GAWTC 8 cut(s) 57, 153, 257, 358, 380, 1735, 2191, 2405
PflFI GACNNNGTC 1 cut(s) 731
PflMI CCANNNNNTGG 1 cut(s) 701
PfoI TCCNGGA 1 cut(s) 553
PinAI ACCGGT 1 cut(s) 503
PkrI GCNGC 6 cut(s) 67, 1031, 1196, 1276, 2361, 2373
PleI GAGTC 4 cut(s) 806, 1009, 1868, 2102
PmaCI CACGTG 1 cut(s) 2272
PmlI CACGTG 1 cut(s) 2272
PpsI GAGTC 4 cut(s) 806, 1009, 1868, 2102
Ppu21I YACGTR 1 cut(s) 2272
PscI ACATGT 2 cut(s) 759, 2316
PshAI GACNNNNGTC 2 cut(s) 637, 1989
Psp6I CCWGG 2 cut(s) 163, 1962
PspCI CACGTG 1 cut(s) 2272
PspEI GGTNACC 3 cut(s) 499, 1211, 1982
PspFI CCCAGC 1 cut(s) 1543
PspGI CCWGG 2 cut(s) 163, 1962
PspN4I GGNNCC 3 cut(s) 752, 1579, 2020
PspPI GGNCC 2 cut(s) 893, 1562
PspXI VCTCGAGB 1 cut(s) 1848
PstI CTGCAG 2 cut(s) 1031, 1354
PsuI RGATCY 4 cut(s) 544, 577, 691, 2461
PsyI GACNNNGTC 1 cut(s) 731
PvuII CAGCTG 1 cut(s) 65
RsaI GTAC 8 cut(s) 185, 200, 746, 957, 1391, 1571, 1579, 2298
RsaNI GTAC 8 cut(s) 184, 199, 745, 956, 1390, 1570, 1578, 2297
RseI CAYNNNNRTG 3 cut(s) 764, 818, 1397
SapI GCTCTTC 1 cut(s) 527
SaqAI TTAA 5 cut(s) 602, 1065, 1296, 1698, 1883
SatI GCNGC 6 cut(s) 66, 1030, 1195, 1275, 2360, 2372
Sau96I GGNCC 2 cut(s) 893, 1562
SchI GAGTC 4 cut(s) 807, 1009, 1868, 2103
ScrFI CCNGG 6 cut(s) 165, 422, 555, 1242, 1964, 2376
SduI GDGCHC 1 cut(s) 539
SfaNI GCATC 4 cut(s) 205, 1693, 2406, 2409
SfcI CTRYAG 2 cut(s) 1027, 1350
Sfr274I CTCGAG 3 cut(s) 772, 1848, 2105
SfuI TTCGAA 1 cut(s) 1009
SinI GGWCC 2 cut(s) 893, 1562
SlaI CTCGAG 3 cut(s) 772, 1848, 2105
SmiMI CAYNNNNRTG 3 cut(s) 764, 818, 1397
SmlI CTYRAG 3 cut(s) 772, 1848, 2105
SmoI CTYRAG 3 cut(s) 772, 1848, 2105
SphI GCATGC 1 cut(s) 942
SsiI CCGC 5 cut(s) 353, 891, 1245, 1980, 2360
SspI AATATT 1 cut(s) 1918
SspMI CTAG 9 cut(s) 296, 860, 903, 1169, 1286, 1331, 1878, 1925, 2207
StyD4I CCNGG 6 cut(s) 163, 420, 553, 1240, 1962, 2374
StyI CCWWGG 1 cut(s) 1453
TaaI ACNGT 1 cut(s) 1488
TaiI ACGT 1 cut(s) 2274
TatI WGTACW 3 cut(s) 744, 1389, 1569
TauI GCSGC 1 cut(s) 2362
TfiI GAWTC 8 cut(s) 57, 153, 257, 358, 380, 1735, 2191, 2405
Tru1I TTAA 5 cut(s) 602, 1065, 1296, 1698, 1883
Tru9I TTAA 5 cut(s) 602, 1065, 1296, 1698, 1883
TscAI CASTG 9 cut(s) 81, 208, 771, 1491, 1522, 1843, 2350, 2392, 2455
TseFI GTSAC 4 cut(s) 79, 458, 679, 1982
TseI GCWGC 5 cut(s) 65, 1029, 1194, 1274, 2371
Tsp45I GTSAC 4 cut(s) 79, 458, 679, 1982
TspGWI ACGGA 4 cut(s) 296, 684, 1008, 1411
TspRI CASTG 9 cut(s) 81, 208, 771, 1491, 1522, 1843, 2350, 2392, 2455
Tth111I GACNNNGTC 1 cut(s) 731
Van91I CCANNNNNTGG 1 cut(s) 701
VpaK11BI GGWCC 2 cut(s) 893, 1562
XapI RAATTY 7 cut(s) 337, 710, 979, 1232, 1624, 1748, 2058
XbaI TCTAGA 2 cut(s) 1168, 1877
XceI RCATGY 4 cut(s) 763, 942, 2177, 2320
XcmI CCANNNNNNNNNTGG 3 cut(s) 1765, 1835, 2165
XhoI CTCGAG 3 cut(s) 772, 1848, 2105
XmnI GAANNNNTTC 3 cut(s) 1646, 1734, 2441
XspI CTAG 9 cut(s) 296, 860, 903, 1169, 1286, 1331, 1878, 1925, 2207
Zsp2I ATGCAT 2 cut(s) 944, 2179
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.