Rh5DG188500

divergent subfamily of APPLE domains

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0000207
Physical Location & Seq
Reverse (-)
20918849 .. 20920331
1483 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG188500.1

Sequence Viewer

Length: 972 bp
ATGAAGCTTGGTTGGAATTTAGTTACTGGTTTAGATAGGTATGTCTCGTCTTGGAGGAGCACAGAAGATCCTGCTCAAGGAGAGTTTTTGCTACAGATGGATACTCATGGTTTCCCACAGATTTTTATTATGAAGGGAGCTAAGATACTGACTAGAGCAGGGACATGGAACGGCGTTGAATTAACTGGATATCAAGGAAAGCCAAATCCAGTAGCGGTTTTTGAGTTTGTGTTGACTAAGGATGAAGTCTATTATGAGTACACACTCCTCAACCGGTCAATGTTTGCAAGATATGTATTAAACCCATATGGCATTGCACAGTGGCTTACTTGGACAGACCACTCACGTAGTTGGGAACCTTTCATCTCAACTCAGGCAGATCAGTGTGAAAATTATGCCTATTGTGGTGCTAATGCTAGATGTAATGTCAGTAACACTCCTGTATGTACATGCTTGAAAGGGTTTGTACCAAAATCTCTGAAAGATTGGAACTCAACAAATTGGTCTGGGGGATGTGTTCGGGAGACTCCATTAGCTTGCAGCTCTACAGATGGCTTCTCAAAGTACAGTAACTTTAAATTGCCAGACACATCTTCTTCCTGGTATGACAAGAGCATGACCCTCAAGGAGTGCCAAGGATTATGTTTGGGAAACTGTTCATGTACTGCATATGCGAATTTAGATTTCAGGGAAGGTGGAAGTGGCTGCTTGCTTTGGTTTGGGAACCTCAATGACATTAGAGAATTCACCTCTGATTCTCAAGACCTCTTTATACGGCTGGCTGCTTCAGACCTAGATCCTATTGTGAAAAAGAGCAAGTCCAGCAAGAAAAAGCTAGCAGGAATTCTGATCGGCTCATCTGTATTTCTTGTGGGAATGCTAATAGTTGGGTTCATTTTGTATAAACGGAAGAGGAAACTCAGAAAGCAAGGTGAGTTTCTCACACATTGGCTCCGGTTTAATTTATTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

323

Amino Acids

36.53

Weight (kDa)

8.83

Isoelectric Point (pI)

30.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
S_locus_glycop PF00954 52 - 159 1.2e-24 S-locus glycoprotein domain
PAN_2 PF08276 180 - 246 3.4e-22 PAN-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000207)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G27300
fragaria_vesca FvH4_3g15930 FvH4_3g15980 FvH4_3g15980 FvH4_3g15980 FvH4_3g15980 FvH4_3g21350 FvH4_3g21400 FvH4_3g21400 FvH4_6g12332
malus_domestica MD02G1167100.v1.1 MD02G1167200.v1.1 MD03G1185600.v1.1 MD05G1213900.v1.1 MD05G1214100.v1.1 MD05G1214200.v1.1 MD05G1214700.v1.1 MD05G1216300.v1.1 MD05G1216800.v1.1 MD05G1217300.v1.1 MD05G1218000.v1.1 MD11G1231100.v1.1 MD11G1231200.v1.1 MD11G1231400.v1.1 MD11G1231500.v1.1 MD11G1232400.v1.1 MD11G1232500.v1.1
prunus_persica Prupe.4G142300_v2.0.a1 Prupe.4G142400_v2.0.a1 Prupe.4G142500_v2.0.a1 Prupe.4G142600_v2.0.a1 Prupe.4G142800_v2.0.a1 Prupe.4G195000_v2.0.a1 Prupe.4G195000_v2.0.a1 Prupe.4G195000_v2.0.a1 Prupe.4G195100_v2.0.a1 Prupe.4G195300_v2.0.a1 Prupe.4G195300_v2.0.a1 Prupe.4G195300_v2.0.a1 Prupe.4G195400_v2.0.a1 Prupe.4G195400_v2.0.a1 Prupe.4G195500_v2.0.a1 Prupe.4G195700_v2.0.a1 Prupe.4G195900_v2.0.a1
pyrus_communis pycom03g16540 pycom05g19920 pycom11g20460 pycom11g20480 pycom11g20490 pycom11g20500 pycom11g20530
rosa_chinensis RchiOBHm_Chr2g0139311 RchiOBHm_Chr2g0139351 RchiOBHm_Chr5g0026801 RchiOBHm_Chr5g0026851 RchiOBHm_Chr5g0026891 RchiOBHm_Chr5g0026911 RchiOBHm_Chr5g0036571 RchiOBHm_Chr5g0036591 RchiOBHm_Chr5g0036721 RchiOBHm_Chr5g0036741 RchiOBHm_Chr7g0222981
rosa_laevigata RLG00000002006 RLG00000019807 RLG00000032964 RLG00000032968 RLG00000032969 RLG00000033706 RLG00000033708 RLG00000033712 RLG00000033716
rosa_multiflora Rmu_co8256651.1_g000001 Rmu_co8279377.1_g000001 Rmu_co8429919.1_g000001 Rmu_co8490103.1_g000001 Rmu_sc0000774.1_g000002 Rmu_sc0002525.1_g000012 Rmu_sc0004223.1_g000008 Rmu_sc0004390.1_g000002 Rmu_sc0006059.1_g000014 Rmu_sc0006059.1_g000024 Rmu_sc0006514.1_g000020 Rmu_sc0014333.1_g000002 Rmu_ssc0000172.1_g000018 Rmu_ssc0000172.1_g000029
rosa_roxburghii Rroxscaffold_1G00044230 Rroxscaffold_1G00044260 Rroxscaffold_1G00044320 Rroxscaffold_1G00044340 Rroxscaffold_1G00052560 Rroxscaffold_1G00052570 Rroxscaffold_1G00052660 Rroxscaffold_1G00052720 Rroxscaffold_2G00105450 Rroxscaffold_2G00105460
rosa_rugosa Rorug02G0356900 Rorug02G0357000 Rorug02G0357100 Rorug02G0357200 Rorug02G0357300 Rorug02G0357400 Rorug02G0357500 Rorug02G0357600 Rorug05G0095400 Rorug05G0095500 Rorug05G0095500 Rorug05G0095600 Rorug05G0095700 Rorug05G0095800 Rorug05G0157700 Rorug05G0157800
rosa_samantha Rh2AG406800 Rh2AG406900 Rh2BG417300 Rh2CG392900 Rh2DG426200 Rh5AG189300 Rh5AG249400 Rh5AG250100 Rh5BG186300 Rh5BG186900 Rh5BG187000 Rh5BG249500 Rh5BG250600 Rh5BG250900 Rh5BG251500 Rh5BG251800 Rh5CG205500 Rh5CG206300 Rh5CG206700 Rh5CG283000 Rh5CG283300 Rh5CG283500 Rh5DG188500 Rh5DG188700 Rh5DG258700 Rh5DG258900 Rh5DG259600 Rh5DG259900 Rh7CG375800 Rh7DG352500
rosa_wichuraiana Rw2G032960 Rw2G032970 Rw2G033220 Rw2G033230 Rw5G017130 Rw5G017230 Rw5G022940 Rw5G022960 Rw5G022980 Rw7G030420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 215
AclWI GGATC 2 cut(s) 62, 791
AcsI RAATTY 4 cut(s) 16, 676, 743, 843
AcuI CTGAAG 1 cut(s) 771
AfaI GTAC 5 cut(s) 260, 448, 468, 566, 664
AfiI CCNNNNNNNGG 1 cut(s) 77
AgeI ACCGGT 1 cut(s) 273
AgsI TTSAA 2 cut(s) 179, 457
AjnI CCWGG 1 cut(s) 599
AluBI AGCT 5 cut(s) 7, 140, 536, 543, 835
AluI AGCT 5 cut(s) 7, 140, 536, 543, 835
Alw21I GWGCWC 1 cut(s) 62
Alw26I GTCTC 2 cut(s) 49, 518
AlwI GGATC 2 cut(s) 62, 791
ApeKI GCWGC 3 cut(s) 540, 705, 782
ApoI RAATTY 4 cut(s) 16, 676, 743, 843
AsiGI ACCGGT 1 cut(s) 273
Asp700I GAANNNNTTC 1 cut(s) 655
AsuHPI GGTGA 2 cut(s) 739, 944
AsuNHI GCTAGC 1 cut(s) 835
Bbv12I GWGCWC 1 cut(s) 62
BbvI GCAGC 3 cut(s) 552, 692, 769
BccI CCATC 2 cut(s) 91, 545
BceAI ACGGC 2 cut(s) 187, 791
BciT130I CCWGG 1 cut(s) 601
BciVI GTATCC 1 cut(s) 94
BcoDI GTCTC 2 cut(s) 49, 518
BfaI CTAG 4 cut(s) 153, 417, 794, 836
BfmI CTRYAG 2 cut(s) 92, 546
BfuI GTATCC 1 cut(s) 94
BisI GCNGC 3 cut(s) 541, 706, 783
BlsI GCNGC 3 cut(s) 542, 707, 784
Bme1390I CCNGG 1 cut(s) 601
BmiI GGNNCC 3 cut(s) 357, 725, 953
BmrFI CCNGG 1 cut(s) 601
BmtI GCTAGC 1 cut(s) 839
BpuEI CTTGAG 3 cut(s) 60, 608, 744
BsaAI YACGTR 1 cut(s) 347
BsaJI CCNNGG 1 cut(s) 634
BsaWI WCCGGW 2 cut(s) 273, 954
BsaXI ACNNNNNCTCC 2 cut(s) 936, 966
Bsc4I CCNNNNNNNGG 1 cut(s) 77
Bse118I RCCGGY 1 cut(s) 273
Bse1I ACTGG 3 cut(s) 31, 190, 209
Bse3DI GCAATG 1 cut(s) 312
BseBI CCWGG 1 cut(s) 601
BseDI CCNNGG 1 cut(s) 634
BseGI GGATG 2 cut(s) 247, 518
BseLI CCNNNNNNNGG 1 cut(s) 77
BseMI GCAATG 1 cut(s) 312
BseMII CTCAG 2 cut(s) 386, 934
BseNI ACTGG 3 cut(s) 31, 190, 209
BseRI GAGGAG 2 cut(s) 70, 257
BseXI GCAGC 3 cut(s) 552, 692, 769
BshTI ACCGGT 1 cut(s) 273
BsiHKAI GWGCWC 1 cut(s) 62
BsiSI CCGG 2 cut(s) 274, 955
BslFI GGGAC 1 cut(s) 175
BslI CCNNNNNNNGG 1 cut(s) 77
BsmAI GTCTC 2 cut(s) 49, 518
BsmFI GGGAC 1 cut(s) 175
BsmI GAATGC 1 cut(s) 882
Bsp1286I GDGCHC 1 cut(s) 62
Bsp1407I TGTACA 1 cut(s) 446
Bsp143I GATC 4 cut(s) 67, 379, 796, 849
BspACI CCGC 1 cut(s) 215
BspCNI CTCAG 2 cut(s) 385, 933
BspLI GGNNCC 3 cut(s) 357, 725, 953
BspOI GCTAGC 1 cut(s) 839
BspPI GGATC 2 cut(s) 62, 791
BsrDI GCAATG 1 cut(s) 312
BsrFI RCCGGY 1 cut(s) 273
BsrGI TGTACA 1 cut(s) 446
BsrI ACTGG 3 cut(s) 31, 190, 209
BssAI RCCGGY 1 cut(s) 273
BssECI CCNNGG 1 cut(s) 634
BssMI GATC 4 cut(s) 67, 379, 796, 849
BssT1I CCWWGG 1 cut(s) 634
Bst2UI CCWGG 1 cut(s) 601
Bst4CI ACNGT 3 cut(s) 321, 569, 656
Bst6I CTCTTC 1 cut(s) 905
BstAUI TGTACA 1 cut(s) 446
BstBAI YACGTR 1 cut(s) 347
BstC8I GCNNGC 4 cut(s) 538, 710, 780, 837
BstDEI CTNAG 4 cut(s) 141, 237, 372, 920
BstENI CCTNNNNNAGG 1 cut(s) 75
BstF5I GGATG 2 cut(s) 247, 518
BstKTI GATC 4 cut(s) 70, 382, 799, 852
BstMAI GTCTC 2 cut(s) 49, 518
BstMBI GATC 4 cut(s) 67, 379, 796, 849
BstMWI GCNNNNNNNGC 1 cut(s) 822
BstNI CCWGG 1 cut(s) 601
BstNSI RCATGY 1 cut(s) 453
BstSCI CCNGG 1 cut(s) 599
BstSFI CTRYAG 2 cut(s) 92, 546
BstV1I GCAGC 3 cut(s) 552, 692, 769
BstX2I RGATCY 2 cut(s) 67, 796
BstYI RGATCY 2 cut(s) 67, 796
BsuI GTATCC 1 cut(s) 94
BtsCI GGATG 2 cut(s) 247, 518
BtsIMutI CAGTG 2 cut(s) 326, 389
Cac8I GCNNGC 4 cut(s) 538, 710, 780, 837
Cfr10I RCCGGY 1 cut(s) 273
Csp6I GTAC 5 cut(s) 259, 447, 467, 565, 663
CspAI ACCGGT 1 cut(s) 273
CviAII CATG 5 cut(s) 107, 165, 450, 616, 660
CviQI GTAC 5 cut(s) 259, 447, 467, 565, 663
DdeI CTNAG 4 cut(s) 141, 237, 372, 920
DpnI GATC 4 cut(s) 69, 381, 798, 851
DpnII GATC 4 cut(s) 67, 379, 796, 849
DraI TTTAAA 1 cut(s) 577
Eam1104I CTCTTC 1 cut(s) 905
EarI CTCTTC 1 cut(s) 905
Eco130I CCWWGG 1 cut(s) 634
Eco32I GATATC 1 cut(s) 191
Eco57I CTGAAG 1 cut(s) 771
EcoNI CCTNNNNNAGG 1 cut(s) 75
EcoRI GAATTC 2 cut(s) 743, 843
EcoRII CCWGG 1 cut(s) 599
EcoRV GATATC 1 cut(s) 191
EcoT14I CCWWGG 1 cut(s) 634
ErhI CCWWGG 1 cut(s) 634
FaeI CATG 5 cut(s) 110, 168, 453, 619, 663
FaqI GGGAC 1 cut(s) 175
FatI CATG 5 cut(s) 106, 164, 449, 615, 659
FauNDI CATATG 2 cut(s) 307, 670
Fnu4HI GCNGC 3 cut(s) 541, 706, 783
FokI GGATG 2 cut(s) 254, 525
Fsp4HI GCNGC 3 cut(s) 541, 706, 783
FspBI CTAG 4 cut(s) 153, 417, 794, 836
GluI GCNGC 3 cut(s) 541, 706, 783
HapII CCGG 2 cut(s) 274, 955
Hin1II CATG 5 cut(s) 110, 168, 453, 619, 663
HincII GTYRAC 1 cut(s) 234
HindII GTYRAC 1 cut(s) 234
HindIII AAGCTT 1 cut(s) 5
HinfI GANTC 2 cut(s) 526, 755
HpaII CCGG 2 cut(s) 274, 955
HphI GGTGA 2 cut(s) 739, 944
Hpy166II GTNNAC 2 cut(s) 234, 261
Hpy188I TCNGA 5 cut(s) 480, 754, 790, 849, 923
Hpy188III TCNNGA 2 cut(s) 521, 761
Hpy8I GTNNAC 2 cut(s) 234, 261
HpyAV CCTTC 2 cut(s) 127, 686
HpyCH4III ACNGT 3 cut(s) 321, 569, 656
HpyCH4IV ACGT 1 cut(s) 346
HpyCH4V TGCA 4 cut(s) 287, 317, 540, 668
HpyF10VI GCNNNNNNNGC 1 cut(s) 822
HpyF3I CTNAG 4 cut(s) 141, 237, 372, 920
HpySE526I ACGT 1 cut(s) 346
Hsp92II CATG 5 cut(s) 110, 168, 453, 619, 663
Kzo9I GATC 4 cut(s) 67, 379, 796, 849
LmnI GCTCC 3 cut(s) 57, 137, 957
Lsp1109I GCAGC 3 cut(s) 552, 692, 769
MaeI CTAG 4 cut(s) 153, 417, 794, 836
MaeII ACGT 1 cut(s) 346
MaeIII GTNAC 3 cut(s) 22, 431, 569
MalI GATC 4 cut(s) 69, 381, 798, 851
MboI GATC 4 cut(s) 67, 379, 796, 849
MboII GAAGA 4 cut(s) 77, 585, 588, 922
MflI RGATCY 2 cut(s) 67, 796
MhlI GDGCHC 1 cut(s) 62
MluCI AATT 9 cut(s) 16, 179, 391, 499, 578, 676, 743, 843, 961
MlyI GAGTC 1 cut(s) 520
MnlI CCTC 7 cut(s) 48, 278, 632, 737, 760, 776, 906
MroXI GAANNNNTTC 1 cut(s) 655
MseI TTAA 4 cut(s) 182, 299, 576, 960
MspI CCGG 2 cut(s) 274, 955
MspR9I CCNGG 1 cut(s) 601
Mva1269I GAATGC 1 cut(s) 882
MvaI CCWGG 1 cut(s) 601
MwoI GCNNNNNNNGC 1 cut(s) 822
NdeI CATATG 2 cut(s) 307, 670
NdeII GATC 4 cut(s) 67, 379, 796, 849
NheI GCTAGC 1 cut(s) 835
NlaIII CATG 5 cut(s) 110, 168, 453, 619, 663
NlaIV GGNNCC 3 cut(s) 357, 725, 953
NspI RCATGY 1 cut(s) 453
PctI GAATGC 1 cut(s) 882
PdmI GAANNNNTTC 1 cut(s) 655
PfeI GAWTC 1 cut(s) 755
PinAI ACCGGT 1 cut(s) 273
PkrI GCNGC 3 cut(s) 542, 707, 784
PleI GAGTC 1 cut(s) 520
PpsI GAGTC 1 cut(s) 520
Ppu21I YACGTR 1 cut(s) 347
Psp6I CCWGG 1 cut(s) 599
PspGI CCWGG 1 cut(s) 599
PspN4I GGNNCC 3 cut(s) 357, 725, 953
PsuI RGATCY 2 cut(s) 67, 796
RsaI GTAC 5 cut(s) 260, 448, 468, 566, 664
RsaNI GTAC 5 cut(s) 259, 447, 467, 565, 663
SaqAI TTAA 4 cut(s) 182, 299, 576, 960
SatI GCNGC 3 cut(s) 541, 706, 783
Sau3AI GATC 4 cut(s) 67, 379, 796, 849
SchI GAGTC 1 cut(s) 520
ScrFI CCNGG 1 cut(s) 601
SduI GDGCHC 1 cut(s) 62
SfcI CTRYAG 2 cut(s) 92, 546
SmlI CTYRAG 3 cut(s) 75, 623, 759
SmoI CTYRAG 3 cut(s) 75, 623, 759
Sse9I AATT 9 cut(s) 16, 179, 391, 499, 578, 676, 743, 843, 961
SsiI CCGC 1 cut(s) 215
SspMI CTAG 4 cut(s) 153, 417, 794, 836
StyD4I CCNGG 1 cut(s) 599
StyI CCWWGG 1 cut(s) 634
TaaI ACNGT 3 cut(s) 321, 569, 656
TaiI ACGT 1 cut(s) 349
TasI AATT 9 cut(s) 16, 179, 391, 499, 578, 676, 743, 843, 961
TatI WGTACW 4 cut(s) 258, 446, 564, 662
TfiI GAWTC 1 cut(s) 755
Tru1I TTAA 4 cut(s) 182, 299, 576, 960
Tru9I TTAA 4 cut(s) 182, 299, 576, 960
TscAI CASTG 2 cut(s) 326, 389
TseI GCWGC 3 cut(s) 540, 705, 782
TspDTI ATGAA 6 cut(s) 17, 146, 258, 352, 648, 883
TspGWI ACGGA 1 cut(s) 922
TspRI CASTG 2 cut(s) 326, 389
XagI CCTNNNNNAGG 1 cut(s) 75
XapI RAATTY 4 cut(s) 16, 676, 743, 843
XceI RCATGY 1 cut(s) 453
XmnI GAANNNNTTC 1 cut(s) 655
XspI CTAG 4 cut(s) 153, 417, 794, 836
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.